样式: 排序: IF: - GO 导出 标记为已读
-
High rates of intestinal colonization with carbapenemase producing Enterobacteriaceae in hematopoietic stem cell transplant recipients. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2024-02-21 Ikram Ayari, Yosra Chebbi, Anis Raddaoui, Dorra Belloumi, Siwar Frigui, Rim Werhni, Tarek Ben Othmen, Nour Abedejlil, Wafa Achour
Carbapenem resistant Enterobacteriaceae (CRE) are major human pathogens because, these cause high number of difficult-to-treat infections. Allogeneic hematopoietic stem cell transplant (AHSCT) recipients are highly exposed to these type of bacteria. The aim of our study was to investigate prevalence of CRE colonization in AHSCT patients and to determine genes encoding carbapenem resistance. A retrospective
-
Carbapenem-resistant Klebsiella pneumoniae in the Balkans: Clonal distribution and associated resistance determinants. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2024-02-21 Maria Chatzidimitriou, Asimoula Kavvada, Dimitrios Kavvadas, Maria Anna Kyriazidi, Konstantinos Eleftheriadis, Sotirios Varlamis, Vassilis Papaliagkas, Stella Mitka
Carbapenems are considered to be among the last line antibiotics against extended-spectrum β-lactamase producing Enterobacterales. Carbapenem-resistant Klebsiella pneumoniae (CRKP) has been frequently reported and its spread in Europe is indisputable and poses an enormous threat to hospitalized patients which is of growing concern. This review aims to record prevalence of CRKP in the Balkan region
-
Pediatric tuberculosis in Mexico and the COVID-19 phenomenon: Past and present. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2024-02-15 Liliana Rivera-Espinosa, Chaak Castellanos-Carrizal, Sergio Montes
In endemic regions, tuberculosis in children constitutes a bigger fraction of total cases as compared to those in low endemic regions, regardless of the implications, this phenomenon has been historically neglected. Pediatric tuberculosis has an insidious onset and quickly develops into disseminated disease and the young are at a special risk for dissemination. Some studies suggest that measures to
-
Prevalence and antimicrobial resistance of bacterial enteropathogens in Crete, Greece, during 2011-2022. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2024-02-12 Sofia Maraki, Viktoria Eirini Mavromanolaki, Dimitra Stafylaki, Evangelia Iliaki-Giannakoudaki, Anna Kasimati
Diarrheal diseases are of great concern worldwide and are responsible for considerable morbidity and mortality. This study investigated the epidemiology and the antibiotic susceptibility of bacterial enteropathogens among diarrheal patients of all ages in Crete, Greece during 2011-2022. Stool specimens were tested by conventional cultural methods for Salmonella, Shigella, Campylobacter, diarrheagenic
-
Investigations of carbapenem resistant and hypervirulent Klebsiella pneumoniae. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2024-01-30 El Mehdi Belouad, Elmostafa Benaissa, Nadia El Mrimar, Yassine Eddair, Adil Maleb, Mostafa Elouennass
Hypervirulent Klebsiella pneumoniae is an emerging pathogen that has gained attention due to its increased ability to cause infections even in healthy individuals. The aim of this study is to investigate virulence factors in K. pneumoniae strains isolated from clinical specimens and their association with carbapenem resistance. The study was conducted on 260 isolates identified between 2018 and 2023
-
Outbreak caused by pandrug-resistant blaOXA-69/blaOXA-23/blaGES harboring Acinetobacter baumannii ST2 in an intensive care unit. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2024-01-29 Anis Raddaoui, Aymen Mabrouk, Yosra Chebbi, Siwar Frigui, Nazek Al-Gallas, Mohamed Salah Abbassi, Wafa Achour
Acinetobacter baumannii has emerged as a main nosocomial pathogen exhibiting high rates of resistance to clinically relevant antibiotics. Six pandrug-resistant A. baumannii (PDR-A. baumannii) were recovered from three patients in a Tunisian Intensive Care Unit (ICU) between 10th and 16th of May 2018 resulting in one fatal case and raising the possibility of an outbreak. On 18th of May environmental
-
Antibiotic resistance and genomic characterization of Mycobacterium abscessus complex isolates from patients with pulmonary tuberculosis in Iran: A multi-center study (2010-2021). Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2024-01-29 Mohammad Hashemzadeh, Aram Asareh Zadegan Dezfuli, AzarDokht Khosravi, Nazanin Ahmad Khosravi, Morteza Saki
One hundred and eighteen sputum specimens suspected of Mycobacterium abscessus infection were collected. Species level identification of M. abscessus was performed by rpoB sequencing. Clonality analysis was done by multilocus sequence typing (MLST) for M. abscessus. Antibiotic susceptibility testing was performed for clarithromycin, amikacin, ciprofloxacin and moxifloxacin. Altogether 128 isolates
-
First detection of a cefiderocol-resistant and extensively drug-resistant Acinetobacter baumannii clinical isolate in Bulgaria. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2024-01-23 Tanya Strateva, Slavil Peykov
Cefiderocol (CFDC) is a first-in-class siderophore cephalosporin with potent activity against multidrug-resistant Gram-negative bacteria including carbapenem-resistant Acinetobacter baumannii. The present study aimed to explore the CFDC resistance mechanisms of an extensively drug-resistant A. baumannii isolate from Bulgaria. The A. baumannii Aba52 strain (designated Aba52) was obtained in 2018 from
-
Hypervirulent Klebsiella pneumoniae: An update on epidemiology, detection and antibiotic resistance. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-12-04 Béla Kocsis
Klebsiella pneumoniae is a major human pathogen as it is responsible for various infections. In the past years hypervirulent K. pneumoniae (hvKP) emerged and disseminated worldwide. In this review a summary will be given about epidemiology, detection and antibiotic resistance of hypervirulent K. pneumoniae. A common feature of hypervirulent K. pneumoniae is a combined expression of several virulence
-
Accurate identification of Streptococcus pseudopneumoniae and other mitis group Streptococci identified as atypical Streptococcus pneumoniae in Tunisian pediatric population. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-12-01 Samar Mhimdi,Khaoula Meftah,Aida Bouafsoun,Hanen Smaoui
Accurate identification of Mitis group streptococci especially Streptococcus pneumoniae and Streptococcus pseudopneumoniae seems difficult due to the lack of specific and sensitive tests. We performed an approach for the identification of atypical pneumococci in pediatric Tunisian population. In this study, 49 streptococcal isolates that were considered as atypical S. pneumoniae were analyzed by: optochin
-
Role of periodontal pathogens in atherosclerotic plaque development and progression: An overview. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-11-16 Amita Rao, Ballamoole Krishna Kumar
Atherosclerosis is a progressive disease marked by the accumulation of lipids and fibrous components in the large arteries. It is one of the primary causes of heart disease and stroke. Periodontal diseases encompass conditions like gingivitis and periodontitis, which are multifactorial diseases associated with dysbiotic plaque biofilms that trigger an immune-inflammatory host response, eventually resulting
-
Molecular epidemiology and antimicrobial resistance of Haemophilus influenzae isolates from otitis media in Bulgaria. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-11-08 Alexandra Sashova Alexandrova, Ivo Nikolaev Sirakov, Lena Petrova Setchanova, Kalina Ionkova Mihova, Daniela Rosenova Pencheva, Raina Tsvetanova Gergova
Haemophilus influenzae is one of the main bacteria responsible for otitis media (OM) among children worldwide. We aimed to estimate the distribution of encapsulated and non-capsulated variants (NTHi), biotypes, antibiotic susceptibility, and molecular epidemiology of H. influenzae isolates recovered from pediatric OM cases in Bulgaria.Capsule detection was done by PCR for bexB gene, absent in NTHi
-
Composition of gut microbiota and its correlations with neurological, intestinal, cardiovascular and metabolic diseases. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-11-08 Morena Sciuto, Roberto Catanzaro
The intestinal microbiota is a microenvironment that has been the subject of studies for several decades. Over time, it has been reconsidered as a possible cofactor of multiple acute and chronic human diseases. In fact, alterations of the intestinal bacterial flora have been found in various neurological diseases. There are three modes of interaction between the intestinal microbiota and the gut-brain-axis:
-
Antimicrobial veterinary drug sale pattern in correlation with critically important antimicrobials for human use: A five-year study in Montenegro. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-10-30 Snežana Mugoša,Vuk Vračar,Nadežda Tešin,Jana Mitrović,Dragana Tomanić,Zorana Kovačević
Antimicrobial resistance (AMR) poses a significant threat in veterinary medicine due to the excessive and inappropriate use of antimicrobial agents, compromising the effectiveness of these drugs. To combat AMR, the collection of data on the consumption of antibiotics is paramount, as there is a well-established connection between antibiotic use and AMR in both humans and food-producing animals. Hence
-
Genetic diversity and biofilm formation of invasive and noninvasive Streptococcus agalactiae isolates: Emergence of hypervirulent CC19 strains in Tehran, Iran. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-10-25 Mehdi Goudarzi,Mehrdad Haghighi,Yousef Mirzaei,Behzad Pourhossein,Masoud Dadashi,Mohammad Javad Nasiri
The prevalence of Streptococcus agalactiae infections in adult populations is increasing. The current study aimed to characterize the genetic features of S. agalactiae strains responsible for different infections. A cross-sectional study was performed on 65 S. agalactiae strains (30 invasive and 35 noninvasive) isolated from non-pregnant women. All S. agalactiae isolates were confirmed by atr and dltS
-
Characterization of disinfectant susceptibility profiles among clinical isolates of Acinetobacter baumannii in Ardabil, Iran. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-10-12 Malek Namaki Kheljan, Malihe Hassanzadeh, Mehran Srdari Jabedar, Ali Mohammadi Gollou, Parastoo Ashouri, Roghayeh Teimourpour, Mohsen Arzanlou
Antimicrobial disinfectants have been extensively used to control hospital-acquired infections worldwide. Prolonged exposure to bacteria could promote resistance to antimicrobial disinfectants. This study evaluated the antimicrobial activity of four commonly used disinfectants; triclosan, chlorhexidine digluconate, benzalkonium chloride, and formaldehyde against Acinetobacter baumannii clinical isolates
-
High-risk clonal lineages among extended-spectrum β-lactamase producing Escherichia coli and Klebsiella pneumoniae from urban and rural stagnant water samples in Tunisia. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-10-12 Amel Romdhani, Sarah Cheriet, Mohamed Salah Abbassi, Sana Lengliz, Paul Hynds, Ilhem Boutiba-Ben Boubaker, Ramzi Boubaker Landolsi
This study sought to investigate the occurrence and subsequently to characterize extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae from urban and rural stagnant water samples during the wet season (December to February) in several regions of northern Tunisia. From 56 stagnant water samples, 14 ESBL-producing Enterobacteriaceae were recovered, including 9 Escherichia coli, 3 Klebsiella
-
Genotype distribution of hepatitis C virus in the province of Gaziantep, a 10-year evaluation. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-10-11 Osman Sezer Cırıt, Yelda Demir, Mehmet Saıt Yıldırım, Buket Alpaslan, Fatma Avcıoglu, Yusuf Doğan, Pınar Astam
Hepatitis C virus (HCV) can cause both acute and chronic hepatitis infections. Gaziantep is located southeast part of Turkey and has a border with Syria. More than 400,000 Syrian refugees live in Gaziantep. The aim of this study was to evaluate distribution of HCV genotypes among Syrian patients and in people who inject drugs.Serum samples form 1,628 individuals (786 female, 842 male) which were sent
-
Molecular characteristics of carbapenem-resistant Raoultella ornithinolytica. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-10-10 Xicai Sun, Yugang Wang, Xuesong Wang, Honggang Wang
Aim of this study was to explore molecular characteristics and resistance mechanisms of carbapenem-resistant Raoultella ornithinolytica (CR-ROR) isolated from patients in a hospital in China. Three CR-ROR strains were collected and bacterial identification was done by Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) Vitek-MS and by digital DDH analysis. VITEK
-
Genetic diversity of Staphylococcus aureus isolated from ear infections in Iran: Emergence of CC8/ST239-SCCmec III as major genotype. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-09-18 Zahra Rahmani, Sareh Sadat Hosseini, Parmida Bagheri, Mehdi Goudarzi
Increase in antibiotic resistance in Staphylococcus aureus isolated from ear infection is a serious public health problem. The objective of this investigation was to determine the antibacterial resistance profile and genetic variability of the S. aureus isolated from adult patients with otitis externa (OE) and otitis media (OM) infections, Tehran- Iran. The disk diffusion was employed to detect the
-
In vitro activity of imipenem/relebactam and ceftazidime/avibactam against carbapenem-resistant Klebsiella pneumoniae from blood cultures in a University hospital in Serbia. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-08-03 Sanja Zornic,Ivana Petrovic,Bojana Lukovic
The study aimed to investigate prevalence of carbapenem-resistant Klebsiella pneumoniae (CRKP) blood culture isolates and their susceptibility to two new antibiotics, imipenem/relebactam and ceftazidime/avibactam. Out of 765 isolates recovered from blood cultures in a tertiary care hospital in Serbia between 2020 and 2023, 143 non-repetitive K. pneumoniae strains were included in this study. Minimum
-
Role of oral microbiota in irreversible pulpitis - Current strategies and future perspectives. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-07-28 Preethesh Shetty,Shishir Shetty,Praveen Rai,Ballamoole Krishna Kumar,Raksha Bhat
Irreversible pulpitis is an inflammation of the tooth pulp caused by an opportunity-driven invasion of the pulp space by oral microbiota typically prevalent in the oral cavity. Microbial organisms are extensively recognised to be the fundamental cause of endodontic infections and treatment failures. Previously, bacterial species responsible for these infections were largely recognised using conventional
-
Detection of carbapenem resistant Pseudomonas aeruginosa co-harboring blaVIM-2 and blaGES-5 in burn patients. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-07-25 Souhir Hmissi, Anis Raddaoui, Siwar Frigui, Mohamed Salah Abbassi, Wafa Achour, Yosra Chebbi, Lamia Thabet
Pseudomonas aeruginosa is one of the major infectious agents in burn patients. Globally, high rates of antimicrobial resistance in P. aeruginosa have been reported, which is a cause of concern. The objective of this study was to determine the rate of resistance to carbapenems in P. aeruginosa isolates recovered from burn patients in Tunisia, to search genes encoding for carbapenemases and to determine
-
Molecular epidemiology and characterization of endemic, epidemic and imported hepatitis A virus (HAV) strains in Hungary (2003-2022). Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-07-25 Gábor Reuter, Ákos Boros, Péter Pankovics
Hepatitis A virus (HAV) is one of the most important etiological agents of acute viral hepatitis but comprehensive molecular epidemiological study with chrono-phylogeographical data are not available from Hungary.Between 2003 and 2022, a total of 8,307 HAV infections were registered officially in Hungary of which 400 (4.8%) HAV IgM antibody-positive serum samples were collected countrywide. HAV genomic
-
Emergence of colistin resistant Acinetobacter baumannii clonal complex 2 (CC2) among hospitalized patients in Iran. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-07-24 Bahareh Hajihashemi,Ali Abbasi,Dariush Shokri
Acinetobacter baumannii is a major causative agent of serious nosocomial infections. This study was carried out to investigate the molecular characterization of colistin resistant isolates of A. baumannii from hospitalized patients, based on multilocus sequence typing (MLST). A cross-sectional study was conducted to collect A. baumannii from clinical samples in Isfahan from 2021 to 2022. Isolates were
-
Analysis of NDM-1 and IMP-8 carbapenemase producing Raoultella planticola clinical isolates. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-07-21 Qingyu Wang,Xinyi Wang,Chunling Chen,Li Zhao,Jie Ma,Kai Dong
Object of our study was to analyze the carriage of resistance genes in carbapenem-resistant Raoultella planticola (CRRP) by whole genome sequencing (WGS). Three strains of CRRP (named WF0027, WF3597 and WF3648) were collected for clinical analysis and susceptibility of antimicrobial agents was determined. The WGS of three strains was done by Illumina platform and strain identification was performed
-
Clinical and microbiological characteristics of nocardiosis: A 5-year single-center study in Crete, Greece. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-07-17 Sofia Maraki, Viktoria Eirini Mavromanolaki, Anna Kasimati, Dimitra Stafylaki, Effie Scoulica
Nocardiosis is a rare disease affecting both immunocompromised and immunocompetent hosts, presented in various clinical forms ranging from localized to disseminated infection. Aim of the present study was to investigate the clinical and microbiological characteristics of nocardiosis, antimicrobial resistance profiles, treatment, and outcomes of Nocardia infection over the last 5 years at our institution
-
Genotypic and phenotypic insights into virulence factors of nosocomial Stenotrophomonas maltophilia isolates collected in Bulgaria (2011-2022). Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-07-04 Tanya Strateva, Angelina Trifonova, Alexander Stratev, Slavil Peykov
The present study aimed to explore the virulence characteristics in 221 Bulgarian nosocomial Stenotrophomonas maltophilia isolates (2011-2022) via screening for the presence of virulence genes, their mutational variability, and the corresponding enzyme activity. PCR amplification, enzymatic assays, whole-genome sequencing (WGS), and biofilm quantification on a polystyrene plate were performed. The
-
Retraction note to: Treatment of methicillin resistant Staphylococcus aureus wound infection using vancomycin-loaded nanoparticles: An in vitro and in vivo study. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-06-27 Hassan Thoulfikar A Alamir,Mustafa Gheni Taher,Mazin Razooqi Mohammed,Saja Hameed Kareem,Abduladheem Turki Jalil
Background Due to the rise of bacteria that are resistant to multiple drugs, treating infections in burn wounds has become a worldwide problem. It is important to find new ways to treat these infections. Lipid-based drug delivery systems have emerged as a promising type of carrier for antibiotics as well as antibacterial agents, such as antimicrobial peptides and nucleic acids, for wound infections
-
Prevalence and distribution of high-risk Human Papillomavirus in Western Türkiye between 2019 and 2022. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-06-22 Gülfem Terek Ece,Doğukan Pira,Gökçe Lara Bodur
Human Papillomavirus (HPV) is a primarily sexually transmitted human pathogen linked with the formation of anogenital warts and several types of cancer including cervical cancer, oropharyngeal cancer, penile cancer, and anal cancer. HPV genotypes are divided into high-risk and low-risk in accordance with their potential for carcinogenesis. The most notable high-risk genotypes are HPV-16 and HPV-18
-
Imipenem resistance associated with amino acid alterations of the OprD porin in Pseudomonas aeruginosa clinical isolates. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-06-21 Maryam Nazari,Hadi Ahmadi,Shahnaz Hosseinzadeh,Amirhossein Sahebkar,Farzad Khademi
Globally, the spread of carbapenem-resistant strains has limited treatment options for multidrug-resistant (MDR) Pseudomonas aeruginosa infections. This study aimed to determine the role of point mutations as well as the expression level of the oprD gene in the emergence of imipenem-resistant P. aeruginosa strains isolated from patients referred to Ardabil hospitals. A total of 48 imipenem-resistant
-
Exploring the complex and multifaceted interplay of the gut microbiome and cancer prevention and therapy. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-06-12 Sukrit Kashyap, Asmita Das
Recent scientific research has indicated that the gut microbiota constitutes a nuanced, diverse ecosystem of microorganisms that have gained significant attention due to its crucial involvement in shaping human health and diseases. In particular, the gut microbiota plays a pivotal role in cancer prevention, and disturbances in its composition and function, known as dysbiosis, that have been linked
-
Pericarditis related to post-acute COVID infection: A case report and review of the literature. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-06-02 Nhu Ngoc Nguyen, Pierre Dudouet, Catherine Dhiver, Philippe Gautret
Cardiovascular involvement has been described in acute and recovered COVID-19 patients. Here, we present a case of symptomatic pericarditis with persistent symptoms for at least six months after the acute infection and report 66 published cases of pericarditis in discharged COVID patients. Patient mean age ± SD was 49.7 ± 13.3 years, ranging from 15 to 75 years and 57.6% were female. A proportion of
-
Association of carbapenem and multidrug resistance with the expression of efflux pump-encoding genes in Pseudomonas aeruginosa clinical isolates. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-05-25 Shaghayegh Yousefi, Maryam Nazari, Rashid Ramazanzadeh, Amirhossein Sahebkar, Elham Safarzadeh, Farzad Khademi
Efflux pumps play an important role in the emergence of antibiotic-resistant Pseudomonas aeruginosa strains. The present study aimed to assess the expression of the MexAB-OprM, MexCD-OprJ, MexEF-OprN, and MexXY-OprM efflux pumps in carbapenem-resistant and multidrug-resistant (MDR) P. aeruginosa strains isolated from clinical specimens between June 2019 and January 2022 in Ardabil city. The presence
-
Frequency of mutations in erm(39) related to clarithromycin resistance and in rrl related to linezolid resistance in clinical isolates of Mycobacterium fortuitum in Iran. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-05-24 Mohammad Hashemzadeh, Aram Asareh Zadegan Dezfuli, Azar Dokht Khosravi, Nazanin Ahmad Khosravi, Abdorazagh Hashemi Shahraki
Mycobacterium fortuitum is a clinically important species among nontuberculous mycobacteria (NTM). Treatment of diseases caused by NTM is challenging. The aim of this study was identification of drug susceptibility and detection of mutations in erm(39) related to clarithromycin resistance and in rrl related to linezolid resistance in clinical isolates of M. fortuitum in Iran. In the study, 328 clinical
-
Molecular epidemiology of carbapenem-resistant Enterobacteriaceae isolated from patients in COVID-19 wards and ICUs in a Bulgarian University Hospital. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-05-18 Dobromira Savova, Denis Niyazi, Milena Bozhkova, Temenuga Stoeva
Many studies report an increase in antimicrobial resistance of Gram - negative bacteria during the COVID-19 pandemic. Our aim was to evaluate the epidemiological relationship between carbapenem-resistant (CR) Enterobacteriaceae isolates from patients in COVID-19 wards and to investigate the main mechanisms of carbapenem resistance in these isolates during the period April 2020-July 2021. A total of
-
High seroprevalence of hepatitis E virus (HEV) in South Transdanubia, Hungary (2010-2022). Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-05-09 Benigna Balázs, Ákos Boros, Péter Pankovics, Gábor Reuter
Hepatitis E virus (HEV) is an increasingly recognized etiological agent of acute, chronic and extrahepatic human infections with primarily zoonotic origin in Europe. Limited numbers of comprehensive population-based studies are available related to HEV seroepidemiology, especially from Central Europe.The aim of this study was to investigate the seroprevalence and trends of total and IgM antibodies
-
Investigation of in vitro efficacy of meropenem/polymyxin B and meropenem/fosfomycin combinations against carbapenem resistant Klebsiella pneumoniae strains. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-05-02 Rıza Adaleti, Yaşar Nakipoğlu, Şeyma Çalık, Neslihan Arıcı, Nilgün Kansak, Seniha Şenbayrak, Sebahat Aksaray
The incidence of infections caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) is increasing worldwide, and very limited number of effective antibiotics are available for therapy. In our study, the in vitro efficacy of meropenem/polymyxin B and meropenem/fosfomycin combinations against CRKP strains was investigated. The efficiency of meropenem/polymyxin B and meropenem/fosfomycin combinations
-
West Nile virus infections in Hungary: Epidemiological update and phylogenetic analysis of the Hungarian virus strains between 2015 and 2022. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-05-02 Anna Nagy,András Horváth,Eszter Mezei,Judit Henczkó,Nóra Magyar,Orsolya Nagy,Anita Koroknai,Nikolett Csonka,Mária Takács
Following the introduction of the West Nile virus (WNV) into Hungary in 2004, it has shortly become one of the most important human arbovirus infections, with a gradually increasing number of cases. The study aimed to summarize the current epidemiological situation in Hungary and sequence the WNV PCR-positive clinical specimens and virus isolates by next-generation whole genome sequencing (NGS) to
-
Prevalence of carbapenemases in Enterobacterales from urine specimens in an university hospital in Istanbul, Turkey. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-04-14 Münevver Sadunoğlu Güler,Gökhan Aygün,Seher Akkuş,Ahmet Mert Kuşkucu,Ömer Küçükbasmacİ,Nevrİye Gönüllü
We aimed to investigate the prevalence of carbapenemases in Enterobacterales strains isolated from urine specimens between July 2019 and July 2020.CIM and modified CIM tests were applied as well as detection of blaOXA-48, blaNDM, blaVIM, blaKPC and blaIMP genes was performed by multiplex PCR.One hundred fifty of 3,242 Enterobacterales strains were found to be carbapenem resistant and 46 were included
-
Prevalence and genetic characteristics of fusidic acid resistant Staphylococcus aureus clinical isolates: Emergence of t030 strains carrying fusB in Tehran, Iran. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-03-23 Mehdi Goudarzi,Simasadat Seyedjavadi,Parmida Bagheri,Masoud Dadashi,Mohammad Javad Nasiri
The literature on fusidic acid resistant Staphylococcus aureus strains is scarce in Iran, although the emergence of these strains in health care settings is increasing. This descriptive cross-sectional study was conducted on 68 fusidic acid resistant S. aureus strains to learn about the molecular characteristics and antimicrobial resistance of strains isolated from hospitalized patients. In the present
-
Molecular characterization and antibiotic resistance of Staphylococcus aureus isolated from clinical specimens in an urban university hospital in Bangkok, Thailand. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-02-14 Siriphan Boonsilp,Jędrzej Sikora,Kitwadee Rupprom,Sarper Acılıoğlu,Anchalee Homkaew,Daranee Nutalai,Uraporn Phumisantiphong,Thanwa Wongsuk
Little is known about the properties of the current strains of Staphylococcus aureus associated with human infections in Thailand. This study examined the rate of resistance to various antimicrobial agents, prevalence of virulence genes, and biofilm formation ability of 60 clinical S. aureus isolates from a single Thai hospital. Moreover, the Staphylococcus protein A gene (spa) type was determined
-
Establishing a molecular laboratory in COVID-19 pandemic: The experience of a regional laboratory in Spain. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-02-08 Zaira Moure, Elena Cuadros, Daniel Pablo-Marcos, María José Reina, Inés de Benito, Ana Belén Campo
The high demand for COVID-19 diagnosis overwhelmed reference hospitals. Regional laboratories had to incorporate molecular technology to respond to the emergency. This work described the implementation of molecular diagnostic tools and the detection of SARS-CoV-2, in a regional hospital with no previous experience, from October 2020 to March 2022. The laboratory structure was significantly modified
-
Staphylococcal biofilm on wedding rings worn by laboratory workers. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-02-01 Ivana Ćirković, Aleksa Pejović, Miloš Jovićević, Snežana Brkić, Slobodanka Djukić, Dragana D Božić
Hands of healthcare workers play essential role in the spreading of antimicrobial-resistant microorganisms in and out of the healthcare settings. Less is known about the role of laboratory workers (LWs). The aim of our study was to evaluate the presence of biofilm-forming staphylococci on the surface of jewelry rings of LWs and their antimicrobial susceptibility pattern.A total of 79 LWs from eight
-
Prevalence and clonal diversity of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius isolated from dogs and cats with eye discharge. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-02-01 Seyda Cengiz, Sitkican Okur, Cihan Oz, Ferda Turgut, Burak Gumurcinler, Nur Selcen Sevuk, Ayse Ilgin Kekec, Haluk Cepoglu, Umit Sevimli, Mehmet Cemal Adiguzel
Methicillin-resistant Staphylococcus (MRS) has originated, spread extensively, and become a prominent source of bacterial infections in both human and animal.
-
Characteristics of multiresistant Pseudomonas aeruginosa isolates from burn patients in Iran. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-01-23 Arash Abednezhad, Bita Bakhshi, Nastaran Asghari Moghadam, Nima Faraji, Elahe Derakhshan-Nezhad, Hajar Mohammadi Barzelighi
Infections caused by multidrug resistant (MDR) Pseudomonas aeruginosa isolates in burn patients restrict therapeutic strategies. The current study aimed to analyze antibiotic resistance genes and multilocus sequence typing (MLST) of P. aeruginosa strains isolated from burn patients in Shahid Motahari hospital in Tehran, Iran.Altogether 63 P. aeruginosa isolates were characterized in this study. Antibiotic
-
Investigation of NDM-1 and OXA-48 producing carbapenem resistant Klebsiella pneumoniae ST15 in Iran. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-01-17 Hedayat Bozorgi Mohajer, Himen Salimizand, Dahieh Gharanizadeh, Afra Hossainpanahi, Rashid Ramazanzadeh
The aim of this study was to determine the frequency of carbapenem resistant Klebsiella pneumoniae (CRKP) sequence types (STs) in Iran. Samples were collected from three university hospitals in Sanandaj, Iran, from December 2016 to March 2018. Antibiotic susceptibility testing, phenotypic and genotypic detection of carbapenemases were performed. Common K. pneumoniae capsular types were sought for all
-
Detection of multidrug-resistant Acinetobacter baumannii from burn patients and healthcare workers in Iran. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-01-13 Farzaneh Firoozeh, Fatemeh Bakhshi, Masoud Dadashi, Farzad Badmasti, Mohammad Zibaei, Narges Omidinia
Multidrug-resistant (MDR) Acinetobacter baumannii is a serious global health threat. Burn patients are at high risk to acquire A. baumannii infections from endogenous sources. This study evaluated carbapenem resistance and clonal relatedness of A. baumannii isolated from burn patients and healthcare workers (HCWs).The study was performed in 100 non-duplicated A. baumannii isolates from nasal and hand
-
Geographic distribution of antibiotic resistance of Helicobacter pylori: A study in Bulgaria. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-01-13 Lyudmila Boyanova, Galina Gergova, Nayden Kandilarov, Liliya Boyanova, Daniel Yordanov, Raina Gergova, Rumyana Markovska
Prevalence of antibiotic resistant Helicobacter pylori was compared between 50 patients living outside the capital city and 50 matched pairs of capital city residents (CCRs). H. pylori isolates from 2018 to 2022 were included. Resistance rates in CCRs and those living elsewhere were 4.0 and 6.0% to amoxicillin, 48.0 and 42.0% to metronidazole, 30 and 30% to clarithromycin, and 4.0 and 4.0% to tetracycline
-
Analysis of biofilm formation in nosocomial Stenotrophomonas maltophilia isolates collected in Bulgaria: An 11-year study (2011-2022). Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-01-13 Tanya Strateva, Angelina Trifonova, Ivo Sirakov, Dayana Borisova, Mikaela Stancheva, Emma Keuleyan, Lena Setchanova, Slavil Peykov
The present study aimed to explore the genotypic and phenotypic characteristics of biofilm formation in Bulgarian nosocomial Stenotrophomonas maltophilia isolates (n = 221) during the period 2011-2022, by screening for the presence of biofilm-associated genes (BAG) (spgM, rmlA and rpfF), their mutational variability, and assessment of the adherent growth on a polystyrene surface. The methodology included:
-
Comparison of performance of LIAISON SARS-CoV-2 antigen assay with RT-PCR during the Omicron wave. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2023-01-09 Neslihan Arici, Nilgün Kansak, Tuğçe Şentürk, Kürşat Baydili, Sebahat Aksaray
Due to the newly emerging Omicron variant, there is a need to re-evaluate the performance of automated antigen tests. Our study aim was to evaluate the performance of the automated Liaison SARS-CoV-2 antigen assay against reverse transcriptase polymerase chain reaction (RT-PCR) in samples with Omicron variant.A prospective study was performed on 373 combined oro-nasopharyngeal samples (NPS) randomly
-
Dissemination of epidemic ST239/ST241-t037-agrI-SCCmecIII methicillin-resistant Staphylococcus aureus in a Tunisian trauma burn intensive care unit. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2022-12-09 Anis Raddaoui,Yosra Chebbi,Ons Bouchami,Siwar Frigui,Amen Allah Messadi,Wafa Achour,Lamia Thabet
Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen causing health care-infections in the world, especially in burns. The aim of this study was to assess the extent of dissemination of MRSA isolated from burn patients in Burn Intensive Care Unit in Tunisia and to evaluate the frequency of virulence and antibiotics resistance genes. Among the 72 S. aureus isolates analyzed in
-
Distribution of virulence determinants among Escherichia coli ST131 and its H30/H30-Rx subclones in Turkey. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2022-12-02 Doğanhan Kadir Er, Devrim Dündar, Hüseyin Uzuner
Extraintestinal pathogenic Escherichia coli (ExPEC) is the leading pathogen in urinary tract infection. In recent years multidrug-resistant B2-ST131 E. coli clonal group has disseminated worldwide. The ST131 and its subclones H30 and H30-Rx have been identified only in a few studies from Turkey. The aim of this study is to investigate the presence of ST131 and its subclones and to analyze their adhesin
-
Cluster of cases due to Shigella flexneri producing CTX-M-15 in Spain. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2022-12-01 Erika Kocsis, José Luis Díaz de Tuesta, Juan Sánchez, Rosaura Santamaría, Manuel Moragas, Silvia Herrera-León, Ramón Cisterna
The aim of our study was to delineate an outbreak of gastroenteritis caused by Shigella flexneri and affecting sixteen persons between May and June 2014 in Bilbao, Spain. All patients exhibited symptoms after consuming kebab in the same kebab shop.The outbreak is described through the clinical cases, the microbiological and molecular genetic diagnosis, and the epidemiologic investigation. Minimum inhibitory
-
Multiple detection of hypermucoviscous and hypervirulent strains of Klebsiella pneumoniae: An emergent health care threat. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2022-11-22 Anna Ventura,Elena Addis,Anna Bertoncelli,Annarita Mazzariol
This study focused on the characterization of 19 hypermucoviscous Klebsiella pneumoniae strains, that were identified from 26 hypermucosal strains. In order to identify hypermucoviscous strains of K. pneumoniae, the string test was applied. This phenotype is known in the literature as one of the virulence factors of this species together with the production of biofilm and other hypervirulence factor
-
Distribution of emm, superantigen and other virulence genotypes and detection of phylogenetic relationships in group A streptococcal isolates. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2022-11-11 Suna Kızılyıldırım,Fatih Köksal,Cansu Önlen Güneri
Group A streptococci are important pathogens with various virulence factors, such as M protein, superantigens, hemolysins, deoxyribonuclease, and proteases. The aims of this study are to investigate the detection of emm genotypes and other virulence genes, such as SAgs, DNase, protease, antibiotic resistance, and phylogenetic relationships in GAS strains isolated from clinical samples.Test strains
-
Respiratory pathogens among ill pilgrims and the potential benefit of using point-of-care rapid molecular diagnostic tools during the Hajj. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2022-11-11 Van-Thuan Hoang,Thi-Loi Dao,Tran Duc Anh Ly,Tassadit Drali,Saber Yezli,Philippe Parola,Vincent Pommier de Santi,Philippe Gautret
We investigated respiratory pathogens among ill Hajj pilgrims from Marseille. We also discuss the potential role of point-of-care (POC) rapid molecular diagnostic tools for this purpose. Clinical data were collected using a standardised questionnaire. Influenza A and B viruses, human rhinovirus and human coronaviruses, Staphylococcus aureus, Streptococcus pneumoniae, Haemophilus influenzae and Klebsiella
-
COVID-19 in elderly: Correlations of viral load, clinical course, laboratory parameters, among patients vaccinated with CoronaVac. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2022-11-11 Sabri Engin Altintop,Tugce Unalan-Altintop,Mustafa Cihangiroglu,Pelin Onarer,Fikriye Milletli-Sezgin,Melih Gozukara,Bilge Gozukara,Erman Zengin
SARS-CoV-2 virus was initially identified in Wuhan, China, in December 2019 and a global pandemic was declared in March 2020 by World Health Organization. COVID-19 disease is characterized with severe pneumonia and hypoxemia, especially in the elderly population. The elderly population was primarily vaccinated with CoronaVac, which is a whole virion inactivated vaccine (Sinovac Biotech, China) in Turkey
-
Ginsenoside Rg1 modulates intestinal microbiota and supports re-generation of immune cells in dexamethasone-treated mice. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2022-11-11 Sabiha Yousuf,He Liu,Zhang Yingshu,Danish Zahid,Hassan Ghayas,Ming Li,Yan Ding,Wenzhe Li
Ginsenoside Rg1 is one of the major ginsenosides found in roots of Panax ginseng and Panax notoginseng. Ginsenoside Rg1 is known to possess various biological activities including immunity enhancement activity. However, it is not clear whether the regulation of immune function by Rg1 is related to the intestinal microbiota. In the present study, the immuno-modulatory and gut microbiota-reshaping effects
-
Genome analysis of clinical isolate of Campylobacter fetus subspecies fetus MMM01 from India reveals genetic determinants of pathogenesis and adaptation. Acta Microbiol. Immunol. Hung. (IF 1.5) Pub Date : 2022-11-07 Deepak Sebastian Pinto,Kattapuni Suresh Prithvisagar,Anusha Rohit,Iddya Karunasagar,Indrani Karunasagar,Ballamoole Krishna Kumar
In this study we report the whole genome sequencing (WGS) based analysis of blood-borne Campylobacter fetus subsp. fetus MMM01 isolated from a diabetic patient to obtain deeper insights in to the virulence and host adaptability. The sequenced genome of C. fetus subsp. fetus MMM01 along with reference genomes retrieved from NCBI was subjected to various in-silico analysis including JSpecies, MLST server