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Pseudocnuella soli gen. nov., sp. nov., a bacterium from soil belonging to the family Chitinophagaceae Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2021-01-15 Soohyun Maeng, Yuna Park, Ju Hyun Han, Sang Eun Lee, Myung Kyum Kim, Gayathri Subramani
A Gram-stain negative, aerobic, rod-shaped, yellow-coloured bacterium, designated strain 17J28-1T, was isolated from soil of Jeju Island, Korea. Optimal growth was found to occur at 30 °C, at pH 6.0 and in the absence of NaCl on R2A at 30 °C. Strain 17J28-1T showed resistance to UV-radiation. The draft genome sequence of strain 17J28-1T is 4804,510 bp long with a 49.4% G + C content. Phylogenetic analyses
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Genomic insight into a novel actinobacterium, Actinomadura rubrisoli sp. nov., reveals high potential for bioactive metabolites Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2021-01-15 Hilal Ay
Isolation of novel actinobacteria from unexplored habitats as potential sources of novel drug leads has utmost importance. During the course of screening arid soil samples for novel actinobacteria, strain H3C3T was isolated from Malatya, Turkey and its taxonomic position was revealed by a genome-based polyphasic approach. Pairwise sequence comparison of the 16S rRNA gene showed that the strain is closely
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Spirosoma taeanense sp. nov., a radiation resistant bacterium isolated from a coastal sand dune Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2021-01-15 Ji Hee Lee, Jong-Hyun Jung, Min-Kyu Kim, Han Na Choe, Chi Nam Seong, Sangyong Lim
An aerobic, Gram-negative, non-motile, non-spore-forming, rod-shaped, and pale yellow-colored bacterial strain, designated TS118T, was isolated from a sand sample obtained from a coastal sand dune after exposure to 3 kGy of gamma radiation. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was a member of the genus Spirosoma and most closely related to Spirosoma metallicum
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Geosmithia associated with hardwood-infesting bark and ambrosia beetles, with the description of three new species from Poland Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2021-01-08 Beata Strzałka, Miroslav Kolařík, Robert Jankowiak
Geosmithia species (Hypocreales, Ascomycota) are associates of bark beetles and other arthropods. One species, Geosmithia morbida, is a virulent tree pathogen of Juglans nigra. To date, 10 Geosmithia spp. from conifer-infesting, and at least 23 species from hardwood associated bark beetles have been reported from Europe. The aim of this study was to survey Geosmithia spp. associated with 18 bark and
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Propylenella binzhouense gen. nov., sp. nov. isolated from activated sludge, and proposal of Propylenellaceae fam. nov. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2021-01-05 Yu-Ling Liu, Dong Meng, Fang Wang, Xiao-Fan Gong, Peng-Fei Gu, Xiang-Yu Fan, Zong-Jun Du, Ji-Dong Zou, Qiang Li
A Gram-stain-negative, non-spore-forming, non-motile, short-rod-shaped, and aerobic bacterial strain (designated L72T) was isolated from propylene oxide saponification wastewater activated sludge obtained from a wastewater treatment facility in Binzhou (Shandong Province, PR China). Strain L72T grew between 25 and 40 °C (optimum growth at 30 °C). The pH range for growth was between 6.0 and 8.0 (optimum
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Correction to: Glaciimonas soli sp. nov., a soil bacterium isolated from the forest of a high elevation mountain Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2021-01-04 Wei-Sheng Huang, Li-Ting Wang, Jun-Ning Sun, Jwo-Sheng Chen, Ssu-Po Huang, Shih-Ting Lin, Lina Huang, Wung Yang Shieh
In the original publication of the article, in the abstract, the JCM number of the strain Glaciimonas soli was incorrectly published as: GS1T (= JCM 33275T = BCRC 81091T).
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Possible role played by the SINE2 element in gene regulation, as demonstrated by differential processing and polyadenylation in avirulent strains of E. histolytica Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2021-01-04 Felipe Padilla-Vaca, Naurú Idalia Vargas-Maya, Fátima Berenice Ramírez-Montiel, Cindy Jazmín Delgado-Galván, Ángeles Rangel-Serrano, Itzel Paramo-Pérez, Fernando Anaya-Velázquez, Bernardo Franco
Entamoeba histolytica represents a useful model in parasitic organisms due to its complex genomic organization and survival mechanisms. To counteract pathogenic organisms, it is necessary to characterize their molecular biology to design new strategies to combat them. In this report, we investigated a less-known genetic element, short interspersed nuclear element 2 (SINE2), that is present in this
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Xylanimonas oleitrophica sp. nov., a novel petroleum hydrocarbon degrading bacterium isolated from an Indian oil reservoir Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2021-01-04 Pradnya D. Nagkirti, Anupama S. Engineer, Prashant K. Dhakephalkar
A novel Gram-stain-positive, aerobic, non-spore-forming, and rod-shaped bacterium designated as PW21was isolated from produced water sampled from an oil reservoir in Limbodara, Gujarat, India. Growth occurred at 30–45 °C (optimum 37 °C), at pH 6.5–9.5 (optimum pH 8.5) and in the presence of 0.5–5.0% (w/v) NaCl [optimum, 3% (w/v)]. Phylogenetic analysis based on the 16S rRNA gene sequences showed that
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Description of Cohnella zeiphila sp. nov., a bacterium isolated from maize callus cultures Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2021-01-03 Eric T. Johnson, Patrick F. Dowd, Christopher D. Skory, Christopher A. Dunlap
A Gram-stain positive, aerobic, motile, rod-shaped bacterium designated as strain CBP-2801T was isolated as a contaminant from a culture containing maize callus in Peoria, Illinois, United States. The strain is unique relative to other Cohnella species due to its slow growth and reduced number of sole carbon sources. Phylogenetic analysis using 16S rRNA indicated that strain CBP-2801T is a Cohnella
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Zongyanglinia huanghaiensis gen. nov., sp. nov., a novel denitrifying bacterium isolated from the yellow sea, and transfer of Pelagicola marinus to Zongyanglinia gen. nov. as Zongyanglinia marinus comb. nov Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2021-01-03 Lili Xu, Ang Liu, Yan-Jiao Zhang
Two Gram-stain-negative, oxidase- and catalase-positive, aerobic strains (CY05T and H18S-6) were isolated from sediment samples of the Yellow Sea, China. The strains were positive for denitrification. Optimum growth was observed at 20 °C, pH 7.5–8.0 and with 2.0%–3.0% NaCl. The predominant cellular fatty acids (> 10%) were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), major respiratory quinone was
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Halalkalirubrum salinum gen. nov., sp. nov., a halophilic archaeon isolated from a saline lake Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2021-01-03 Zhenqiang Zuo, Dahe Zhao, Jian Zhou, Jing Han, Hua Xiang
A novel extremely halophilic archaeon, strain N1521T, was isolated from a saline lake in Tibet, China. Cells of the strain were pleomorphic and Gram-stain-negative. It produced red pigments. Growth was observed at 4–42 °C (optimum, 37 °C), pH 7.0–10.5 (optimum, 8.0–9.5), NaCl 11%–25% (optimum, 15%) and in the presence of 0–0.1 M MgCl2 (optimum, 0.05 M) in aerobic conditions. The minimum NaCl concentration
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Description of Salinimonas profundi sp. nov., a deep-sea bacterium harboring a transposon Tn6333 Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2021-01-03 Dao-Feng Zhang, Xi-Wen Cui, Wen-Jun Li, Xiao-Mei Zhang, Hua-Peng Xue, Jian-Ke Huang, Ai-Hua Zhang
A Gram-staining-negative bacterium, strain HHU 13199T, was isolated from a marine sediment sample collected from South China Sea (119°19.896’E, 19°41.569’N) at a depth of 2918 m. The 16S rRNA gene sequence analysis indicated that strain HHU 13199T represents a member of the genus Salinimonas with the highest sequence similarity (99.8%) to the type strain S. iocasae KX18D6T. However, the average nucleotide
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Description and genomic characterization of Streptococcus symci sp. nov., isolated from a child’s oropharynx Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2021-01-02 He Qi, Defeng Liu, Yang Zou, Nan Wang, Han Tian, Chunling Xiao
Using the culturomics approach, we isolated a new Streptococcus species, strain C17T, from the oropharynx mucosa sample of a healthy 5-year-old child living in Shenyang, China. We studied the phenotypic, phylogenetic, and genomic characteristics of strain C17T, which was identified as a Gram-positive, coccus-shaped, non-motile, aerobic, catalase-negative bacteria. Its growth temperatures ranged from
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Archaeal communities of low and high microbial abundance sponges inhabiting the remote western Indian Ocean island of Mayotte Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-12-28 Ana Rita Moura Polónia, Daniel Francis Richard Cleary, Anne Gauvin‐Bialecki, Nicole Joy de Voogd
Marine sponges are abundant and ecologically important components of coral reefs and have been shown to harbour exceptionally high microbial densities, which can differ substantially among sponge species. However, this dichotomy between high and low microbial abundance (HMA, LMA) sponges is still not fully understood, particularly as concerns the archaeal community. This study aims to fill this gap
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Pestalotiopsis trachicarpicola , a novel pathogen causes twig blight of Pinus bungeana (Pinaceae: Pinoideae) in China Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-11-27 Mei Qi, Chen-Xiao Xie, Qian-Wen Chen, Zhong-Dong Yu
Pinus bungeana is one of indigenous trees in China and widely distributed in poor and arid regions for vegetation and industrial woody use. However, since a high-incidence disease threatens the growth of mature P. bungeana tree in the garden and in the plantation every year, the overwintering shoots were infected and died in the next spring with a ratio over 70%, but the cause was beyond understood
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Additions to the genus Gimesia : description of Gimesia alba sp. nov., Gimesia algae sp. nov., Gimesia aquarii sp. nov., Gimesia aquatilis sp. nov., Gimesia fumaroli sp. nov. and Gimesia panareensis sp. nov., isolated from aquatic habitats of the Northern Hemisphere Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-11-24 Sandra Wiegand, Mareike Jogler, Christian Boedeker, Anja Heuer, Patrick Rast, Stijn H. Peeters, Mike S. M. Jetten, Anne-Kristin Kaster, Manfred Rohde, Nicolai Kallscheuer, Christian Jogler
Thirteen novel planctomycetal strains were isolated from five different aquatic sampling locations. These comprise the hydrothermal vent system close to Panarea Island (Italy), a biofilm on the surface of kelp at Monterey Bay (CA, USA), sediment and algae on Mallorca Island (Spain) and Helgoland Island (Germany), as well as a seawater aquarium in Braunschweig, Germany. All strains were shown to belong
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Paenibacillus polymyxa NMA1017 as a potential biocontrol agent of Phytophthora tropicalis, causal agent of cacao black pod rot in Chiapas, Mexico Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-11-23 Belén Chávez-Ramírez, Nadia Denisse Rodríguez-Velázquez, Cecilia Melisa Mondragón-Talonia, Carlos Hugo Avendaño-Arrazate, Misael Martínez-Bolaños, María Soledad Vásquez-Murrieta, Paulina Estrada de los Santos
Cacao represents an important source of income for farmers in the south of Mexico. However, phytosanitary problems have disrupted the production over the years. The use of antagonistic microorganisms as biocontrol agents might improve the production of cacao. In this study, Paenibacillus polymyxa NMA1017, isolated from the rhizosphere of Opuntia ficus-indica L., was used as a biocontrol agent for black
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Pseudonocardia cytotoxica sp. nov., a novel actinomycete isolated from an Arctic fjord with potential to produce cytotoxic compound Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-11-23 Mohandas Dhaneesha, Md. Umar, Titus Susan Merlin, Kottekkatu Padinchati Krishnan, Vrinda Sukumaran, Rupesh Kumar Sinha, Abdulaziz Anas, Peng Fu, John Bryan MacMillan, Thavarool Puthiyedathu Sajeevan
Herein we report the isolation of a novel actinomycete, strain MCCB 268T, from the sediment sample collected from a high Arctic fjord Kongsfjorden. MCCB 268T showed greater than 97% 16S rRNA gene sequence similarity with those of Pseudonocardia konjuensis LM 157T (98.06%), Pseudonocardia soli NW8-21 (97.22%) Pseudonocardia endophytica YIM 56035 (97.08%) and Pseudonocardia nantongensis KLBMP 1282 (97
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Isolation, characterisation and genome analysis of a novel ginsenosides hydrolysing bacterium Ginsengibacter hankyongi gen. nov., sp. nov. isolated from soil Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-11-23 Muhammad Zubair Siddiqi, Md. Amdadul Huq, Wan-Taek Im
A novel yellow-pigmented bacterial strain (designated BR5-29T), was isolated and its taxonomy was studied. Phylogenetic study based on the 16S rRNA and draft genome sequence placed the strain BR5-29T in a distinct lineage within the family Chitinophagaceae, sharing ≤ 93.4% sequence similarity with members of the closely related genera Ferruginibacter, Flavisolibacter, Flavitalea and Niastella. The
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Interplay between differential competition and actions of spore-vectors explain host exclusivity of saprobic fungi in Protea flowers Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-11-22 Vuledzani O. Mukwevho, Léanne L. Dreyer, Francois Roets
Protea flowers host saprobic Knoxdaviesia and Sporothrix fungi that are dispersed by pollinating insects and birds. Different Protea species contain sympatric populations of different fungal species. For example, P. repens host S. splendens and K. proteae, while P. neriifolia host K. capensis and S. phasma. Even though all fungi can grow vigorously on alternative hosts and they share the same spore
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Metabolic activity and bioweathering properties of yeasts isolated from different supraglacial environments of Antarctica and Himalaya Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-11-20 Aritri Sanyal, Runa Antony, Preethika Ganesan, Meloth Thamban
Yeasts have been frequently isolated from cold habitats, but their contribution to essential ecological processes such as the mineralization of organic matter in these environments is less known. Here, the diversity, metabolic capability, and extracellular enzyme profiles of yeasts from snow, blue ice and cryoconite hole environments from East Antarctica and cryoconite holes from a glacier in Western
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Proposal of Thermoactinomyces mirandus sp. nov., a filamentous, anaerobic bacterium isolated from a biogas plant Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-11-19 Mira Mutschlechner, Nina Lackner, Rudolf Markt, Willi Salvenmoser, Christopher A. Dunlap, Andreas O. Wagner
We isolated a filamentous, thermophilic, and first anaerobic representative of the genus Thermoactinomyces, designated strain AMNI-1T, from a biogas plant in Tyrol, Austria and report the results of a phenotypic, genetic, and phylogenetic investigation. Strain AMNI-1T was observed to form a white branching mycelium that aggregates into pellets when grown in liquid medium. Cells could primarily utilize
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Streptomyces buecherae sp. nov., an actinomycete isolated from multiple bat species Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-11-16 Paris S. Hamm, Christopher A. Dunlap, Michael W. Mullowney, Nicole A. Caimi, Neil L. Kelleher, Regan J. Thomson, Andrea Porras-Alfaro, Diana E. Northup
A putative novel clade within the genus Streptomyces was discovered following antifungal screening against Pseudogymnoascus destructans, the causative agent of white-nose syndrome, and described using multi-locus sequencing analysis. Swabs from both the cave myotis bat (Myotis velifer) and the Brazilian free-tailed bat (Tadarida brasiliensis) in southern New Mexico bore isolates AC536, AC541T and AC563
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The assessment of leading traits in the taxonomy of the Bacillus cereus group Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-11-12 Mariano A. Torres Manno, Guillermo D. Repizo, Christian Magni, Christopher A. Dunlap, Martín Espariz
Bacillus cereus sensu lato strains (B. cereus group) are widely distributed in nature and have received interest for decades due to their importance in insect pest management, food production and their positive and negative repercussions in human health. Consideration of practical uses such as virulence, physiology, morphology, or ill-defined features have been applied to describe and classify species
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Biocontrol and plant growth-promoting potentiality of bacteria isolated from compost extract Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-11-06 Imen Zouari, Fatma Masmoudi, Khaled Medhioub, Slim Tounsi, Mohamed Trigui
The use of compost extracts is steadily increasing, offering an attractive way for plant growth enhancement and disease management replacing chemical pesticides. In this study, potential mechanisms involved in plant growth promotion and suppressive activity against fungal diseases, of a compost extract produced from poultry manure/olive husk compost, were investigated. Results of physico-chemical and
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Chronic wasting associated with Chlamydia pneumoniae in three ex situ breeding facilities for tropical frogs Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-11-05 Tobias Eisenberg, Ahmad Fawzy, Ute Kaim, Anne Nesseler, Karin Riße, Iris Völker, Silke Hechinger, Nicole Schauerte, Christina Geiger, Tobias Knauf-Witzens, Ingo Schwabe, Christiane Schnee, Elisabeth Liebler-Tenorio, Can Imirzalioglu, Reinhard Sting
A number of different Chlamydia spp. have been detected in the class Amphibia with C. pneumoniae being the predominant species involved. Chlamydiae have been linked to mass mortality events, thereby representing significant pathogens that deserve attention with respect to worldwide amphibian decline. We here present six cases of chlamydiosis and asymptomatic chlamydial infections in different frog
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Updates to the recently introduced family Lacipirellulaceae in the phylum Planctomycetes : isolation of strains belonging to the novel genera Aeoliella , Botrimarina , Pirellulimonas and Pseudobythopirellula and the novel species Bythopirellula polymerisocia and Posidoniimonas corsicana Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-11-05 Sandra Wiegand, Mareike Jogler, Christian Boedeker, Anja Heuer, Stijn H. Peeters, Nicolai Kallscheuer, Mike S. M. Jetten, Anne-Kristin Kaster, Manfred Rohde, Christian Jogler
Eight novel strains of the phylum Planctomycetes were isolated from different aquatic habitats. Among these habitats were the hydrothermal vent system close to Panarea Island, a public beach at Mallorca Island, the shore of Costa Brava (Spain), and three sites with brackish water in the Baltic Sea. The genome sizes of the novel strains range from 4.33 to 6.29 Mb with DNA G+C contents between 52.8 and
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Vallicoccus soli gen. nov., sp. nov., a novel actinobacterium isolated from soil, and description of Vallicoccaceae fam. nov., Motilibacterales ord. nov. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-11-05 Yu-Zhou Feng, Wei-Xun Chunyu, Rui Liang, Richard L. Hahnke, Peter Schumann, Yu-Rong Zhao, Sheng Gao, Min Yin, Yang Ji, Le Sun, Xiao-Yang Zhi, Shu-Kun Tang
A novel Gram-stain-positive, aerobic, cocci-shaped actinobacterium, designated YIM 75000T, was isolated from a soil sample collected from a dry-hot river valley in Yunnan Province, P.R. China. Growth was observed at 10–45 °C (optimal 37 °C), 0–8% (w/v) NaCl (optimal at 0–3% NaCl) and pH 6.0–8.0 (optimal at pH 7.3). The peptidoglycan contained LL-diaminopimelic acid, glycine, glutamic acid as well as
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Methylomonas rhizoryzae sp. nov., a type I methanotroph isolated from the rhizosphere soil of rice Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-11-04 Pingping Zhu, Minggen Cheng, Dongmei Pei, Yongchuang Liu, Xin Yan
A gammaproteobacterial methanotroph, strain GJ1T, was isolated from a rhizosphere soil sample of rice in Nanjing, China. The cells were Gram-negative, motile rods with a single polar flagellum, and they contained type I intracytoplasmic membranes. The cells formed pink colonies. The strain possessed both the particulate methane monooxygenase enzyme (pMMO) and the soluble methane monooxygenase enzyme
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Spirosoma aureum sp. nov., and Hymenobacter russus sp. nov., radiation-resistant bacteria in Cytophagales order isolated from soil Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-11-04 Soohyun Maeng, Yuna Park, Joo Hyun Han, Sang Eun Lee, Jing Zhang, Min-Kyu Kim, In-Tae Cha, Ki-eun Lee, Byoung-Hee Lee, Myung Kyum Kim
A Gram-stain-negative, aerobic, nonmotile, yellow-colored strain BT328T and Gram-stain-negative, aerobic, non-motile, red-colored strain BT18T were isolated from the soil collected in Korea. Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain BT328T formed a distinct lineage within the family Spirosomaceae (order Cytophagales, class Cytophagia) and was most closely related to
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Rhodocytophaga rosea sp. nov. and Nibribacter ruber sp. nov., two radiation-resistant bacteria isolated from soil Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-11-02 Yuna Park, Soohyun Maeng, Joo Hyun Han, Sang Eun Lee, Jing Zhang, Min-Kyu Kim, In-Tae Cha, Ki-eun Lee, Byoung-Hee Lee, Myung Kyum Kim
Two bacterial strains, 172606-1T and BT10T, were isolated from soil, Korea. Both strains were Gram-stain-negative and rod-shaped bacteria. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain 172606-1T formed a distinct lineage within the family Cytophagaceae (order Cytophagales, class Cytophagia, phylum Bacteroidetes). Strain 172606-1T was most closely related to a member of the
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Human gut microbiota/microbiome in health and diseases: a review Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-11-02 Eman Zakaria Gomaa
The human gut microbiota has received considerable interest in the recent years and our knowledge of the inhabitant species and their potential applications is increased particularly after the development of metagenomic studies. Gut microbiota is highly diverse and harboring trillions of microorganisms in human digestive system. The shaping and multiplication of gut microbiome starts at birth, while
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Diversity of culturable bacteria isolated from ancestral crops of Arica and Parinacota Region, Atacama Desert Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-11-02 Patricio A. Muñoz, Mabel J. Arismendi, Steffany F. Cárdenas, Denise E. Cifuentes Bachmann, Francisca A. Venegas, Germán F. Sepúlveda-Chavera
Arica and Parinacota Region is located at the extreme north of the Atacama Desert, where the high levels of salts and boron, lack of rain, high UV radiation, among other conditions, make this zone an extreme environment. Despite these characteristics, in the transversal valleys, different types of crops are cultivated in this region, which are associated to beneficial microorganisms with specific traits
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Characterization and pathogenicity of Aeromonas veronii associated with mortality in cage farmed grass carp, Ctenopharyngodon idella (Valenciennes, 1844) from the Central Himalayan region of India Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-10-30 Sumanta Kumar Mallik, Nupur Joshi, Neetu Shahi, Krishna Kala, Shivam Singh, Abhay Kumar Giri, Kushagra Pant, Suresh Chandra
In the study, Aeromonas strains (n = 12) were isolated from moribund grass carp fry reared in the cage culture unit from the Central Himalayan region of India. They were identified as Aeromonas veronii, by biochemically and 16S rRNA analysis. The experimental bath infection of grass carp fry was performed using A. veronii GCAFBLC 228, one of the 12 isolates at cell concentrations 106 and 108 CFU mL−1
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Genomic and phenotypic characterization of Bacillus velezensis AMB-y1; a potential probiotic to control pathogens in aquaculture Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-10-23 Arafah M. Emam, Christopher A. Dunlap
The aim of this investigation was to isolate and identify Bacillus species isolated from the internal microbiota of Red sea stingrays as potential probiotics. An initial assay on the ability of the isolates to control stingray pathogens of Vibrio species led to the selection of one highly antagonistic isolate. The most potent isolate was identified based on whole genome phylogeny as Bacillus velezensis
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Exploration of yeast communities in fresh coconut, palmyra, and nipa palm saps and ethanol-fermenting ability of isolated yeasts Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-10-20 Savitree Limtong, Somjit Am-In, Rungluk Kaewwichian, Chutima Kaewkrajay, Sasitorn Jindamorakot
This study aimed to explore communities and the ethanol-fermenting ability of yeasts in fresh coconut, palmyra, and nipa palm saps. From the 90 samples of coconut, palmyra, and nipa palm saps, 204 yeast isolates were isolated and identified as 15 species in the phylum Ascomycota and a species (one strain) in Basidiomycota. Saccharomyces cerevisiae, Hanseniaspora guilliermondii, and Lachancea thermotolerans
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Delineating yeast species with genome average nucleotide identity: a calibration of ANI with haplontic, heterothallic Metschnikowia species Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-10-13 Marc-André Lachance, Dong Kyung Lee, Tom Hsiang
We determined pairwise average nucleotide identity (ANI) values for the genomes of 71 strains assigned to 36 Metschnikowia species, 28 of which were represented by multiple isolates selected to represent the range of genetic diversity of the species, and most of which were defined on the basis of reproductive isolation. Similar to what has been proposed for prokaryote species delineation, an ANI value
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Microbispora cellulosiformans sp. nov., a novel actinomycete with cellulase activity isolated from soil in the cold region Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-10-12 Xiujie Gong, Wensheng Xiang, Xu Cao, Yang Yu, Yubo Hao, Liang Li, Qiuju Wang, Hongtao Zou, Chunrong Qian
A novel cellulase-producing actinomycete strain Gxj-6T, isolated from soil in the cold region (Heihe city, Heilongjiang province, the northernmost part of China), subjected to a taxonomic study using a polyphasic approach. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain Gxj-6T fell within the clade comprising the type strains of species of the genus Microbispora
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Resistance of a Halobacterium salinarum isolate from a solar saltern to cadmium, lead, nickel, zinc, and copper Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-09-24 Houda Baati, Mariem Siala, Chafai Azri, Emna Ammar, Christopher Dunlap, Mohamed Trigui
The current study focuses on the tolerance of a strain of Halobacterium salinarum isolated from Sfax solar saltern (Tunisia) towards cadmium (Cd), lead (Pb), nickel (Ni), zinc (Zn), and copper (Cu) by using agar dilution methods in complex and minimal media. The results showed the least inhibitory metals based on Minimum Inhibitory Concentrations (MICs) were lead (MIC = 4.5 mM), cadmium (MIC = 4 mM)
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Limnobacter alexandrii sp. nov., a thiosulfate-oxidizing, heterotrophic and EPS-bearing Burkholderiaceae isolated from cultivable phycosphere microbiota of toxic Alexandrium catenella LZT09. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-09-21 Yuhan Duan,Zhiwei Jiang,Zheng Wu,Zhi Sheng,Xi Yang,Jingya Sun,Xiaoling Zhang,Qiao Yang,Xinwei Yu,Jianbo Yan
A novel Gram-negative, aerobic, motile and short rod-shaped bacterium with exopolysaccharides production, designated as LZ-4T, was isolated from cultivable phycosphere microbiota of harmful algal blooms-causing marine dinoflagellate Alexandrium catenella LZT09 which produces paralytic shellfish poisoning toxins. Strain LZ-4T was able to use thiosulfate (optimum concentration 10 mM) as energy source
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Isolation and genomic characterization of a pathogenic Providencia rettgeri strain G0519 in turtle Trachemys scripta. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-09-20 Minghao Ye,Xiucai Hu,Aijun Lü,Jingfeng Sun,Chengxun Chen
Providencia rettgeri infection has occurred occasionally in aquaculture, but is rare in turtles. Here, a pathogenic P. rettgeri strain G0519 was isolated from a diseased slider turtle (Trachemys scripta) in China, and qPCR assay was established for the RTX toxin (rtxD) gene. Histopathological examination showed that many inflammatory cells were infiltrated into heart, liver and intestine, as well as
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Sphingomonas palmae sp. nov. and Sphingomonas gellani sp. nov., endophytically associated phyllosphere bacteria isolated from economically important crop plants. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-09-19 Munusamy Madhaiyan,Venkatakrishnan Sivaraj Saravanan,Joseph S Wirth,Tan Hian Hwee Alex,Soo-Jin Kim,Hang-Yeon Weon,Soon-Wo Kwon,William B Whitman,Lianghui Ji
In this study, two endophytic bacterial strains designated JS21-1T and S6-262T isolated from leaves of Elaeis guineensis and stem tissues of Jatropha curcas respectively, were subjected for polyphasic taxonomic approach. On R2A medium, colonies of strains JS21-1T and S6-262T are orange and yellow, respectively. Phylogenetic analyses using 16S rRNA gene sequencing and whole-genome sequences placed the
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Paludisphaera soli sp. nov., a new member of the family Isosphaeraceae isolated from high altitude soil in the Western Himalaya. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-09-16 Rishabh Kaushik,Meesha Sharma,Kumar Gaurav,U Jagadeeshwari,A Shabbir,Ch Sasikala,Ch V Ramana,Maharaj K Pandit
A novel strain of Planctomycetes, designated JC670T, was isolated from a high altitude (~ 2900 m above sea level) soil sample collected from Garhwal region in the Western Himalaya. Colonies of this strain were observed to be light pink coloured with spherical to oval shaped cells having crateriform structures distributed all over the cell surface. The cells divide by budding. Strain JC670T was found
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Bacillus rugosus sp. nov. producer of a diketopiperazine antimicrobial, isolated from marine sponge Spongia officinalis L. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-09-16 Dhruba Bhattacharya,Sergio de Los Santos Villalobos,Valeria Valenzuela Ruiz,Joseph Selvin,Joydeep Mukherjee
A novel Gram-positive and endospore-forming bacterium assigned as strain SPB7T which is also a new source of a cyclic diketopiperazine (3S,6S)-3,6-diisobutylpiperazine-2,5-dione is described. A polyphasic (biochemical, phenotypic and genotypic) approach was used to clarify the taxonomic affiliation of this strain. The partial and complete 16S rRNA gene sequences revealed that strain SPB7T is a member
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Lactobacillus gastricus BTM 7 prevents intestinal colonization by biofilm forming Cronobacter sakazakii in Caenorhabditis elegans model host. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-09-12 Sharma Kavita,Murugesan Pooranachithra,Niharika Singh,Mani Iyer Prasanth,Krishnaswamy Balamurugan,Gunjan Goel
The study reports protective role of potential probiotic cultures against infection by biofilm forming Cronobacter sakazakii in Caenorhabditis elegans model system. Among the fifteen indigenous potential probiotics, the cell free supernatant of Lactobacillus gastricus BTM7 possessed highest antimicrobial action and biofilm inhibition against C. sakazakii. The competitive exclusion assays revealed that
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Effect of kitasamycin and nitrofurantoin at subinhibitory concentrations on quorum sensing regulated traits of Chromobacterium violaceum. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-09-05 Yasser Musa Ibrahim,Ahmed Megahed Abouwarda,Farghaly Abdelhamid Omar
Quorum sensing (QS) is a mechanism of intercellular communication in bacteria that received substantial attention as alternate strategy for combating bacterial resistance and the development of new anti-infective agents. The present investigation reports on the assessment of using subinhibitory concentrations of antibiotics for the inhibition of QS-regulated phenotypes in Chromobacterium violaceum
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Correction to: Duganella rivus sp. nov., Duganella fentianensis sp. nov., Duganella qianjiadongensis sp. nov. and Massilia guangdongensis sp. nov., isolated from subtropical streams in China and reclassification of all species within genus Pseudoduganella. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-08-27 Hui-Bin Lu,Zhi-Peng Cai,Yong-Gang Yang,Mei-Ying Xu
In the published version of the article, the title should have read ‘Duganella rivi sp. nov., Duganella fentianensis sp. nov., Duganella qianjiadongensis sp. nov. and Massilia guangdongensis sp. nov., isolated from subtropical streams in China and reclassification of all species within genus Pseudoduganella’.
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Entomogenous fungi isolated from Cryptotympana atrata with antibacterial and antifungal activity. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-08-27 Xiaoqiu Cui,Huiyun Wang,Hao Yang,Xiao Li,Xiuzhen Li
Although many entomogenous fungi have been discovered over the years, few studies on the crude extracts of fungi isolated from Cryptotympana atrata with antibacterial and antifungal activity were reported. In this study, total twenty entomogenous fungi were isolated for the first time. And among of them, two pure cultures were identified as Purpureocillium lilacinum and Aspergillus fumigatus with apparent
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Three Planctomycetes isolated from biotic surfaces in the Mediterranean Sea and the Pacific Ocean constitute the novel species Symmachiella dynata gen. nov., sp. nov. and Symmachiella macrocystis sp. nov. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-08-24 Markus Salbreiter,Muhammad Waqqas,Mareike Jogler,Nicolai Kallscheuer,Sandra Wiegand,Stijn H Peeters,Anja Heuer,Mike S M Jetten,Christian Boedeker,Patrick Rast,Manfred Rohde,Christian Jogler
Planctomycetes is a phylum of environmentally important bacteria, which also receive significant attention due to their fascinating cell biology. Access to axenic Planctomycete cultures is crucial to study cell biological features within this phylum in further detail. In this study, we characterise three novel strains, Mal52T, Pan258 and CA54T, which were isolated close to the coasts of the islands
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Pan-genome analysis of Paenibacillus polymyxa strains reveals the mechanism of plant growth promotion and biocontrol. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-08-20 Liangliang Zhou,Ting Zhang,Shan Tang,Xueqin Fu,Shuijing Yu
Rapid development of gene sequencing technologies has led to an exponential increase in microbial sequencing data. Genome research of a single organism does not capture the changes in the characteristics of genetic information within a species. Pan-genome analysis gives us a broader perspective to study the complete genetic information of a species. Paenibacillus polymyxa is a Gram-positive bacterium
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Control of the rubber anthracnose fungus Colletotrichum gloeosporioides using culture filtrate extract from Streptomyces deccanensis QY-3. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-08-19 Liushuang Gu,Kai Zhang,Nan Zhang,Xiaoyu Li,Zhiqiang Liu
Colletotrichum gloeosporioides is a main cause of rubber anthracnose, which results in a huge loss for the natural rubber industry. In this study, an actinomycete strain QY-3 was isolated and had good antagonistic activity against C. gloeosporioides with an inhibition rate of 86.6%. Strain QY-3 was identified as Streptomyces deccanensis preliminarily. Millet medium was selected as the optimal fermentation
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Metagenomic profiling of the community structure, diversity, and nutrient pathways of bacterial endophytes in maize plant. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-08-14 Ayomide Emmanuel Fadiji,Ayansina Segun Ayangbenro,Olubukola Oluranti Babalola
This study investigated the diversity, structure and nutrient pathways of the root-associated bacterial endophytes of maize plant cultivated using different fertilizers to verify the claim that inorganic fertilizers have some toxic effects on plant microbiome and not are ecofriendly. Whole DNA was extracted from the roots of maize plants cultivated with organic fertilizer, inorganic fertilizer and
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Stieleria varia sp. nov., isolated from wood particles in the Baltic Sea, constitutes a novel species in the family Pirellulaceae within the phylum Planctomycetes. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-08-14 Frank Surup,Sandra Wiegand,Christian Boedeker,Anja Heuer,Stijn H Peeters,Mareike Jogler,Mike S M Jetten,Manfred Rohde,Christian Jogler,Nicolai Kallscheuer
Species belonging to the bacterial phylum Planctomycetes are ubiquitous members of the microbial communities in aquatic environments and are frequently isolated from various biotic and abiotic surfaces in marine and limnic water bodies. Planctomycetes have large genomes of up to 12.4 Mb, follow complex lifestyles and display an uncommon cell biology; features which motivate the investigation of members
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Effect of different stimuli on twitching behavior of endophytic bacteria isolated from Loranthus sp. Jacq. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-08-13 Rajsekhar Adhikary,Smriti Kundu,Pulak Kumar Maiti,Prashanta Kumar Mitra,Sukhendu Mandal,Vivekananda Mandal
Bacteria need to adopt to different behavioral tuning depending on the dynamic eco-physiological conditions they are exposed to. One of these adaptive strategies is the use of motility. Here we report the twitching motility response of four endophytic isolates of Bacillus sp. when exposed to different eco-physiological stimuli like different nutrient sources, and mechanical and chemical antagonists
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Sphingomonas hominis sp. nov., isolated from hair of a 21-year-old girl. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-08-11 Dao-Feng Zhang,Xi-Wen Cui,Zhe Zhao,Ai-Hua Zhang,Jian-Ke Huang,Wen-Jun Li
A Gram-stain-negative, aerobic, motile strain, HHU CXWT, was isolated from hair of a healthy 21-year-old female student of Hohai University, Nanjing, China. The 16S rRNA gene sequence analysis indicated that HHU CXWT represents a member of the genus Sphingomonas with the highest sequence similarity (97.6%) to the type strain S. aquatilis JSS7T. HHU CXWT grew at 4–35 °C and pH 6–8, with optimum growth
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Administration of indigenous probiotics modulate high-fat diet-induced metabolic syndrome in Sprague Dawley rats. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-07-06 Sakshi Khanna,Sanisha Walia,Kanthi Kiran Kondepudi,Geeta Shukla
Modulation of the gut microbiota by probiotics, is emerging as a promising approach for the management of metabolic diseases but due to their species and strain specific response, isolation of new probiotic strains is gaining importance. The present study was designed to assess the effect of isolated and well characterised indigenous probiotics, Lactobacillus pentosus GSSK2, Lactobacillus fermentum
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Isolation and characterization of plant growth-promoting rhizobacteria and their effects on the growth of Medicago sativa L. under salinity conditions. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-06-20 Zhiyu Zhu,Huanhuan Zhang,Jing Leng,Huanqing Niu,Xiaochun Chen,Dong Liu,Yong Chen,Nan Gao,Hanjie Ying
Plant growth-promoting rhizobacteria are a group of free-living bacteria that colonize plant rhizosphere and benefit plant root growth, thereby increasing host plant to cope with salinity induced stress. The aim of this study was to (1) isolate and characterize auxin-producing bacteria showing a high plant growth-promoting (PGP) potential, and (2) evaluate the PGP effects on the growth of Medicago
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Molecular characterisation of hydrogen sulfide negative Salmonella enterica serovar Havana. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-06-30 İnci Başak Müştak,Hamit Kaan Müştak,Seyyide Sarıçam
Hydrogen sulfide (H2S) detection is a screening method for distinguishing and identifying Salmonella strains from other bacteria in the intestine. Incidences of H2S-negative Salmonella have recently been reported in different countries. Although a high resistance rate against antimicrobial agents has been reported for H2S-positive Salmonella in many regions of the world, there is increasing evidence
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Mesorhizobium terrae sp. nov., a novel species isolated from soil in Jangsu, Korea. Antonie van Leeuwenhoek (IF 2.674) Pub Date : 2020-06-20