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Revealing the salinity adaptation mechanism in halotolerant bacterium Egicoccus halophilus EGI 80432 T by physiological analysis and comparative transcriptomics Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-24 Dai-Di Chen, Bao-Zhu Fang, Ahmad Manzoor, Yong-Hong Liu, Li Li, Osama Abdalla Abdelshafy Mohamad, Wen-Sheng Shu, Wen-Jun Li
Egicoccus halophilus EGI 80432T, a halotolerant bacterium isolated from a saline-alkaline soil, belongs to a member of the class Nitriliruptoria, which exhibits high adaptability to salt environments. At present, the detailed knowledge of the salinity adaptation strategies of Nitriliruptoria was limited except for one research by using comparative genomics analysis. Here, we investigated the salinity
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Micromonospora species from rarely-exploited Egyptian habitats: chemical profile, antimicrobial, and antitumor activities through antioxidant property Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-24 Mohamed S. Nafie, Noha M. Awad, Hend M. Tag, Ibrahim M. Abd El-Salam, Mohamed K. Diab, Sahar A. El-Shatoury
Abstract The development of new anticancer agents with a selective action mechanism has become a significant scientific challenge, especially as cancers remain the world’s leading cause of death. Actinobacteria and its bioactive compounds have recently become a promising perspective alternative to cancer therapy. In this study, some extracted metabolites of Micromonospora exhibited potent antimicrobial
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Modifications of diketopiperazines assembled by cyclodipeptide synthases with cytochrome P 450 enzymes Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-24 Lauritz Harken, Shu-Ming Li
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Gut microbiota alterations associated with antibody-mediated rejection after kidney transplantation Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-24 Junpeng Wang, Xin Li, Xiaoqiang Wu, Zhiwei Wang, Chan Zhang, Guanghui Cao, Shun Liu, Tianzhong Yan
Abstract Antibody-mediated rejection (AMR) has become the major challenge for kidney transplantation, and the efficacy of existing therapies was limited to prevent AMR. Increasing evidences have demonstrated the link between gut microbiota alterations and allograft outcome. However, there has been no comprehensive analysis to profile the gut microbiota associated with AMR after kidney transplantation
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Correction to: Transcriptome analysis of the thermotolerant yeast Kluyveromyces marxianus CCT 7735 under ethanol stress Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-22 Raphael Hermano Santos Diniz, Juan C. Villada, Mariana Caroline Tocantins Alvim, Pedro Marcus Pereira Vidigal, Nívea Moreira Vieira, Mónica Lamas-Maceiras, María Esperanza Cerdán, María-Isabel González-Siso, Petri-Jaan Lahtvee, Wendel Batista da Silveira
A Correction to this paper has been published: https://doi.org/10.1007/s00253-021-11185-2
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Enhanced in vitro anticancer activity of yeast expressed recombinant glucose oxidase versus commercial enzyme Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-22 Evelyn Martínez-Mora, María Del Rosario González-González, Xristo Zarate, Pilar Carranza-Rosales, Mónica A. Ramírez-Cabrera, Isaías Balderas-Rentería, Eder Arredondo-Espinoza
Abstract Cancer treatments continue to have many disadvantages. Reactive oxygen species, such as H2O2, in high concentrations, can cause cytotoxicity to cells, being even greater in cancer cells. One of the H2O2-producing enzymes is glucose oxidase; its application in cancer treatment should be explored. In this work, the extracellular expression of the mutated recombinant enzyme glucose oxidase was
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Degradation of the low-calorie sugar substitute 5-ketofructose by different bacteria Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-22 Jacqueline Schiessl, Konrad Kosciow, Laura S. Garschagen, Juliane J. Hoffmann, Julia Heymuth, Thomas Franke, Uwe Deppenmeier
Abstract There is an increasing public awareness about the danger of dietary sugars with respect to their caloric contribution to the diet and the rise of overweight throughout the world. Therefore, low-calorie sugar substitutes are of high interest to replace sugar in foods and beverages. A promising alternative to natural sugars and artificial sweeteners is the fructose derivative 5-keto-D-fructose
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Response characteristics of the membrane integrity and physiological activities of the mutant strain Y217 under exogenous butanol stress Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-19 Yue Gao, Xiang Zhou, Miao-Miao Zhang, Ya-Jun Liu, Xiao-Peng Guo, Cai-Rong Lei, Wen-Jian Li, Dong Lu
Abstract Butanol inhibits bacterial activity by destroying the cell membrane of Clostridium acetobutylicum strains and altering functionality. Butanol toxicity also results in destruction of the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS), thereby preventing glucose transport and phosphorylation and inhibiting transmembrane transport and assimilation of sugars, amino acids, and
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The storage stability of Bacillus subtilis spore displaying cysteine protease of Clonorchis sinensis and its effect on improving the gut microbiota of mice Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-19 Zeli Tang, Zhanshuai Wu, Hengchang Sun, Lu Zhao, Mei Shang, Mengchen Shi, Hongye Jiang, Zhipeng Lin, Xinyi Zhou, Xuerong Li, Xinbing Yu, Yan Huang
Bacillus subtilis (B. subtilis) spore can serve as an ideal vehicle for expressing heterologous antigens, and elicit specific immune responses by oral administration. In previous studies, we successfully constructed the recombinant B. subtilis spores expressing cysteine protease of Clonorchis sinensis (C. sinensis, B.s-CsCP), and confirmed that oral administration of B.s-CsCP could elicit good protective
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Production of β-glucan exopolysaccharide lasiodiplodan by Lasiodiplodia theobromae CCT 3966 from corn bran acid hydrolysate Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-18 Rafael Rodrigues Philippini, Sabrina Evelin Martiniano, Paulo Ricardo Franco Marcelino, Anuj Kumar Chandel, Júlio César dos Santos, Silvio Silvério Da Silva
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Advances in monitoring and control of refolding kinetics combining PAT and modeling Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-17 Jan Niklas Pauk, Janani Raju Palanisamy, Julian Kager, Krisztina Koczka, Gerald Berghammer, Christoph Herwig, Lukas Veiter
Abstract Overexpression of recombinant proteins in Escherichia coli results in misfolded and non-active protein aggregates in the cytoplasm, so-called inclusion bodies (IB). In recent years, a change in the mindset regarding IBs could be observed: IBs are no longer considered an unwanted waste product, but a valid alternative to produce a product with high yield, purity, and stability in short process
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Biogenesis of silver nanoparticles to treat cancer, diabetes, and microbial infections: a mechanistic overview Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-16 Bilal Javed, Muhammad Ikram, Fatima Farooq, Tahira Sultana, Zia-ur-Rehman Mashwani, Naveed Iqbal Raja
Abstract Green synthesis of silver nanoparticles (SNPs) by harnessing the natural abilities of plant secondary metabolites has advantages over routine physical and chemical synthetic approaches due to their one-step experimental setup to reduce and stabilize the bulk silver into SNPs, biocompatible nature, and therapeutic significance. The unique size, shape, and biochemical functional corona of SNPs
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Major aldehyde dehydrogenase AldFGH of Gluconacetobacter diazotrophicus is independent of pyrroloquinoline quinone but dependent on molybdopterin for acetic acid fermentation Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-16 Roni Miah, Shun Nina, Takeru Murate, Naoya Kataoka, Minenosuke Matsutani, Kazunobu Matsushita, Toshiharu Yakushi
Abstract Acetic acid fermentation involves the oxidation of ethanol to acetic acid via acetaldehyde as the intermediate and is catalyzed by the membrane-bound alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) of acetic acid bacteria. Although ADH depends on pyrroloquinoline quinone (PQQ), the prosthetic group associated with ALDH remains a matter of debate. This study aimed to address the
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A MALDI-TOF MS database for fast identification of Vibrio spp. potentially pathogenic to marine mollusks Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-15 M. Moussa, E. Cauvin, A. Le Piouffle, O. Lucas, A. Bidault, C. Paillard, F. Benoit, B. Thuillier, M. Treilles, M. A. Travers, Céline Garcia
Abstract In mollusk aquaculture, a large number of Vibrio species are considered major pathogens. Conventional methods based on DNA amplification and sequencing used to accurately identify Vibrio species are unsuitable for monitoring programs because they are time-consuming and expensive. The aim of this study was, therefore, to develop the MALDI-TOF MS method in order to establish a rapid identification
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Diverse β-lactam antibiotic-resistant bacteria and microbial community in milk from mastitic cows Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-15 Zhengxin Ma, Shinyoung Lee, Peixin Fan, Yuting Zhai, Jaehyun Lim, Klibs N. Galvão, Corwin Nelson, Kwangcheol Casey Jeong
Abstract Intramammary bacterial infection, the most common cause of mastitis, is the most costly disease in dairy cattle in the US and reason for antibiotic usage. Ceftiofur, a third-generation cephalosporin, is generally used to treat such disease, but it has a high treatment failure rate. Though the reason is not known clearly, it is hypothesized that multiple factors are associated with the treatment
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Selection of entomopathogenic fungus Beauveria bassiana ( Deuteromycotina : Hyphomycetes ) for the biocontrol of Dendroctonus ponderosae ( Coleoptera : Curculionidae , Scolytinae ) in Western Canada Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-15 Albert Remus R. Rosana, Stanley Pokorny, Jennifer G. Klutsch, Cherry Ibarra-Romero, Randy Sanichar, Daniel Engelhardt, Marco J. van Belkum, Nadir Erbilgin, Joerg Bohlmann, Allan L. Carroll, John C. Vederas
Abstract The mountain pine beetle, Dendroctonus ponderosae, has infested over ~16 Mha of pine forests in British Columbia killing >50% of mature lodgepole pine, Pinus contorta, trees in affected stands. At present, it is functionally an invasive species in Alberta, killing and reproducing in evolutionarily naïve populations of lodgepole pine (P. contorta), novel jack pine (P. banksiana), and their
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Diagnosis of histoplasmosis: current status and perspectives Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-15 María Agustina Toscanini, Alejandro David Nusblat, María Luján Cuestas
Abstract Histoplasmosis is a worldwide-distributed systemic mycosis caused by the dimorphic fungus Histoplasma capsulatum. Its clinical manifestations range from subclinical or mild respiratory illness to progressive disseminated histoplasmosis (PDH), a life-threatening disease, whose accurate diagnosis is still challenging and limited in many countries, where this disease is highly endemic. In this
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Antibacterial and safety tests of a flexible cold atmospheric plasma device for the stimulation of wound healing Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-15 Bouke Boekema, Matthea Stoop, Marcel Vlig, Jos van Liempt, Ana Sobota, Magda Ulrich, Esther Middelkoop
Abstract Cold atmospheric plasma (CAP) devices generate an ionized gas with highly reactive species and electric fields at ambient air pressure and temperature. A flexible dielectric barrier discharge (DBD) was developed as an alternative antimicrobial treatment for chronic wounds. Treatment of Staphylococcus aureus in collagen-elastin matrices with CAP for 2 min resulted in a 4 log reduction. CAP
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Assembly of a Rieske non-heme iron oxygenase multicomponent system from Phenylobacterium immobile E DSM 1986 enables pyrazon cis -dihydroxylation in E. coli Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-13 Andreas Hunold, Wendy Escobedo-Hinojosa, Elsa Potoudis, Daniela Resende, Theresa Farr, Per-Olof Syrén, Bernhard Hauer
Abstract Phenylobacterium immobile strain E is a soil bacterium with a striking metabolism relying on xenobiotics, such as the herbicide pyrazon, as sole carbon source instead of more bioavailable molecules. Pyrazon is a heterocyclic aromatic compound of environmental concern and its biodegradation pathway has only been reported in P. immobile. The multicomponent pyrazon oxygenase (PPO), a Rieske non-heme
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Tracking changes in adaptation to suspension growth for MDCK cells: cell growth correlates with levels of metabolites, enzymes and proteins Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-13 Sabine Pech, Markus Rehberg, Robert Janke, Dirk Benndorf, Yvonne Genzel, Thilo Muth, Albert Sickmann, Erdmann Rapp, Udo Reichl
Abstract Adaptations of animal cells to growth in suspension culture concern in particular viral vaccine production, where very specific aspects of virus-host cell interaction need to be taken into account to achieve high cell specific yields and overall process productivity. So far, the complexity of alterations on the metabolism, enzyme, and proteome level required for adaptation is only poorly understood
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Toxin-antitoxin systems and their medical applications: current status and future perspective Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-13 Akriti Srivastava, Soumya Pati, Himani Kaushik, Shailja Singh, Lalit C. Garg
Abstract Almost all bacteria synthesize two types of toxins—one for its survival by regulating different cellular processes and another as a strategy to interact with host cells for pathogenesis. Usually, “bacterial toxins” are contemplated as virulence factors that harm the host organism. However, toxins produced by bacteria, as a survival strategy against the host, also hamper its cellular processes
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Lactonic sophorolipid–induced apoptosis in human HepG2 cells through the Caspase-3 pathway Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-13 Xiao Wang, Na Xu, Qinglin Li, Shengqi Chen, Hui Cheng, Mo Yang, Ting Jiang, Jun Chu, Xiaojing Ma, Dengke Yin
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Inducing red pigment and inhibiting citrinin production by adding lanthanum(III) ion in Monascus purpureus fermentation Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-12 Hai-qing Liu, Zhen-feng Huang, Shan-zhong Yang, Xiao-fei Tian, Zhen-qiang Wu
Abstract Monascus pigments (MPs) are widely used natural colorants in Asian countries. The problems of low extracellular red pigment (ERP) and high citrinin remain to be solved in Monascus pigment production. The effect of lanthanum(III) ion (LaCl3) on Monascus purpureus fermentation was investigated in this study. The yields of ERP and biomass respectively reached maxima of 124.10 U/mL and 33.10 g/L
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Helicobacter pylori infection worsens impaired glucose regulation in high-fat diet mice in association with an altered gut microbiome and metabolome Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-12 Chao Peng, Xinbo Xu, Zichuan He, Nianshuang Li, Yaobin Ouyang, Yin Zhu, Nonghua Lu, Cong He
Abstract Emerging evidence suggests that Helicobacter pylori infection is associated with metabolic disorders, although the underlying mechanisms are poorly defined. This study aimed to investigate the interaction among H. pylori, a high-fat diet (HFD), and the gut microbiota with glucose regulation and alterations in microbial metabolites. Mice were randomly allocated to H. pylori-infected and noninfected
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Identification of new proteins related with cisplatin resistance in Saccharomyces cerevisiae Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-12 Antonio M. Burgos-Molina, Silvia Mercado-Sáenz, Casimiro Cárdenas, Beatriz López-Díaz, Francisco Sendra-Portero, Miguel J. Ruiz-Gómez
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The role of transport proteins in the production of microbial glycolipid biosurfactants Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-12 Silke Claus, Liam Jenkins Sánchez, Inge Noëlle Adrienne Van Bogaert
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Detection and identification of Xanthomonas campestris pv. campestris and pv. raphani by multiplex polymerase chain reaction using specific primers Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-12 Yasuhiro Inoue, Takashi Fujikawa, Yuichi Takikawa
Abstract Black rot and bacterial spots threaten the cultivation of cruciferous vegetables worldwide, and the development of a method that can easily detect, identify, and distinguish their respective pathogens Xanthomonas campestris pv. campestris (Xcc) and X. campestris pv. raphani (Xcr) is required. Multiple whole-genome sequences of Xcc and Xcr were aligned to identify specific regions and subsequently
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Host defence peptides identified in human apolipoprotein B as promising antifungal agents Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-12 Eliana Dell’Olmo, Rosa Gaglione, Angela Cesaro, Valeria Cafaro, Wieke R. Teertstra, Hans de Cock, Eugenio Notomista, Henk P. Haagsman, Edwin J. A. Veldhuizen, Angela Arciello
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Effect of pluronic block polymers and N-acetylcysteine culture media additives on growth rate and fatty acid composition of six marine microalgae species Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-12 Justine Sauvage, Gary H. Wikfors, Xiaoxu Li, Mark Gluis, Nancy Nevejan, Koen Sabbe, Alyssa Joyce
Abstract The efficiency of microalgal biomass production is a determining factor for the economic competitiveness of microalgae-based industries. N-acetylcysteine (NAC) and pluronic block polymers are two compounds of interest as novel culture media constituents because of their respective protective properties against oxidative stress and shear-stress-induced cell damage. Here we quantify the effect
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Engineering of primary metabolic pathways for titer improvement of milbemycins in Streptomyces bingchenggensis Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-10 Yuqing Liu, Haiyan Wang, Shanshan Li, Yanyan Zhang, Xu Cheng, Wensheng Xiang, Xiangjing Wang
Abstract Milbemycins are used commercially as insect repellents and acaricides; however, their high cost remains a significant challenge to commercial production. Hence, improving the titer of milbemycins for commercial application is an urgent priority. The present study aimed to effectively increase the titer of milbemycins using a combination of genome re-sequencing and metabolic engineering. First
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Substrate properties as controlling parameters in attached algal cultivation Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-10 Zahra Karimi, H. Dail Laughinghouse, Virginia A. Davis, David M. Blersch
Abstract There is growing interest in attached algae cultivation systems because they could provide a more cost- and energy-efficient alternative to planktonic (suspended algae) cultivation systems for many applications. However, attached growth systems have been far less studied than planktonic systems and have largely emphasized algae strains of most interest for biofuels. New algal biorefinery pathways
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Structure-based substrate specificity analysis of GH11 xylanase from Streptomyces olivaceoviridis E-86 Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-10 Zui Fujimoto, Naomi Kishine, Koji Teramoto, Sosyu Tsutsui, Satoshi Kaneko
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An efficient system for stable markerless integration of large biosynthetic gene clusters into Streptomyces chromosomes Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-10 Dominika Csolleiova, Renata Knirschova, Bronislava Rezuchova, Dagmar Homerova, Beatrica Sevcikova, Maria Matulova, Luz Elena Núñez, Renata Novakova, Lubomira Feckova, Rachel Javorova, Jesús Cortés, Jan Kormanec
Abstract The bacteria of the genus Streptomyces are among the most important producers of biologically active secondary metabolites. Moreover, recent genomic sequence data have shown their enormous genetic potential for new natural products, although many new biosynthetic gene clusters (BGCs) are silent. Therefore, efficient and stable genome modification techniques are needed to activate their production
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Research progress and the biotechnological applications of multienzyme complex Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-10 Yi Jiang, Xinyi Zhang, Haibo Yuan, Di Huang, Ruiming Wang, Hongling Liu, Tengfei Wang
Abstract The multienzyme complex system has become a research focus in synthetic biology due to its highly efficient overall catalytic ability and has been applied to various fields. Multienzyme complexes are formed by cascading complexes, which are multiple functionally related enzymes that continuously and efficiently catalyze the production of substrates. Compared with current mainstream microbial
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Calcium-crosslinked alginate-encapsulated bacteria for remediating of cadmium-polluted water and production of CdS nanoparticles Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-09 Shaozu Xu, Xuesong Luo, Qiaoyun Huang, Wenli Chen
Abstract Pollution with the heavy metal cadmium (Cd2+) is a global problem. Cadmium adversely affects living organisms, highlighting the need to develop new methods for removal of this pollutant from the environment. In this study, we used a novel biomaterial based on calcium-crosslinked alginate-encapsulated bacteria to precipitate Cd2+ in polluted water. Our results show that calcium-crosslinked
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Comparison of the fermentation and bacterial community in the colon of Hu sheep fed a low-grain, non-pelleted, or pelleted high-grain diet Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-09 Limei Lin, Ehab Bo Trabi, Fei Xie, Shengyong Mao
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Effect of cross-linked enzyme aggregate strategy on characterization of sn-1,3 extracellular lipase from Aspergillus niger GZUF36 Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-09 Ruonan Zhu, Cuiqin Li, Cuicui Chen, Shuqi Xing, Yangyang Cai, Xuefeng Zeng, Laping He
Abstract The sn-1,3 extracellular lipase from Aspergillus niger GZUF36 (EXANL1) has important potential applications. The cross-linked enzyme aggregate (CLEA) of purified EXANL1 (CLEA-EXANL1) achieved optimum activity recovery (148.5 ± 0.9%), immobilization yield (100 ± 0%), and recovered activity (99.7 ± 0.6%) with 80% tert-butanol as the precipitant, glutaraldehyde (GA) concentration of 30 mM, GA
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Seed coating as a delivery system for the endophyte Trichoderma koningiopsis Th003 in rice ( Oryza sativa ) Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-09 Diego Cortés-Rojas, Camilo Beltrán-Acosta, Yimmy Zapata-Narvaez, Martha Chaparro, Martha Gómez, Mauricio Cruz-Barrera
Seed coating is a technique to cover seeds with external agents to upgrade their performance, handling, and plant establishment. Plant beneficial microbes (PBMs), such as plant growth-promoting bacteria, mycorrhizal fungi, and other fungi (e.g., Trichoderma spp.), decrease agrochemical inputs, enhance tolerance to biotic-abiotic stresses, and increase essential plant nutrition. The demand for pre-treated
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The formation of fungus - serpentine aggregation and its immobilization of lead(II) under acidic conditions Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-08 Chengfeng Yu, Luting Zhang, Shameer Syed, Ying Li, Min Xu, Bin Lian
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Panorama of intron dynamics and gene rearrangements in the phylum Basidiomycota as revealed by the complete mitochondrial genome of Turbinellus floccosus Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-08 Jie Cheng, Qing Luo, Yuanhang Ren, Zhou Luo, Wenlong Liao, Xu Wang, Qiang Li
Abstract In the present study, the complete mitogenome of Turbinellus floccosus was sequenced, assembled, and compared with other basidiomycete mitogenomes. The mitogenome of T. floccosus consists of a circular DNA molecule, with a size of 62,846 bp. Gene arrangement analysis indicated that large-scale gene rearrangements occurred in the levels of family and genus of basidiomycete species, and the
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Metagenomic insights into the effect of sulfate on enhanced biological phosphorus removal Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-08 Norihisa Matsuura, Yalkhin Masakke, Smruthi Karthikeyan, Sui Kanazawa, Ryo Honda, Ryoko Yamamoto-Ikemoto, Konstantinos T. Konstantinidis
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Contribution of pathogenic fungi to N 2 O emissions increases temporally in intensively managed strawberry cropping soil Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-08 Ying Huang, Jinquan Jing, Meiling Yan, Christina Hazard, Yuehong Chen, Chengbao Guo, Xu Xiao, Jiujun Lin
Abstract Intensively managed agriculture land is a significant contributor to nitrous oxide (N2O) emissions, which adds to global warming and the depletion of the ozone layer. Recent studies have suggested that fungal dominant N2O production may be promoted by pathogenic fungi under high nitrogen fertilization and continuous cropping. Here, we measured the contribution of fungal communities to N2O
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Thiamine-producing lactic acid bacteria and their potential use in the prevention of neurodegenerative diseases Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-06 María del Milagro Teran, Alejandra de Moreno de LeBlanc, Graciela Savoy de Giori, Jean Guy LeBlanc
Abstract Thiamine or vitamin B1, an essential micronutrient mainly involved in energy production, has a beneficial impact on the nervous system, and its deficiency can be associated with the development and progression of neurodegenerative diseases. The aim of this work was to select thiamine-producing lactic acid bacteria (LAB) and study their physiological effects using neuron cell cultures. In this
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Characterization and function of membrane vesicles in Gram-positive bacteria Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-06 Yina Cao, Huancai Lin
Abstract In recent years, extracellular vesicles have gained more attention. However, studies on membrane vesicles in Gram-positive bacteria were carried out relatively late because of the thick bacterial wall and the low production of membrane vesicles. Thanks to the research in recent years, the cognition of the composition and function of the membrane vesicles of Gram-positive bacteria has made
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Spatial and seasonal variations of sediment bacterial communities in a river-bay system in South China Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-05 Zhongyi Lu, Zongbao Liu, Cuijing Zhang, Qiaoyan Wei, Siyu Zhang, Meng Li
Abstract River-bay systems are transitional areas that hold important roles in biogeochemical processes between continents and oceans. However, composition and structure of microbial communities shaped by such environments have not been clear yet. In this study, we used high-throughput sequencing of 16S rRNA genes to analyze the diversity and composition of sediment bacterial communities from the Shenzhen
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An ATP-free in vitro synthetic enzymatic biosystem facilitating one-pot stoichiometric conversion of starch to mannitol Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-05 Xinlei Wei, Qiangzi Li, Congcong Hu, Chun You
Abstract D-Mannitol (hereinafter as mannitol) is a six-carbon sugar alcohol with diverse applications in food and pharmaceutical industries. To overcome the drawbacks of the chemical hydrogenation method commonly used for mannitol production at present, there is a need to search for novel prospective mannitol production strategies that are of high yield and low cost. In this study, we present a novel
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Comparative proteomic analyses reveal the metabolic aspects and biotechnological potential of nitrate assimilation in the yeast Dekkera bruxellensis Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-04 Irina Charlot Peña-Moreno, Denise Castro Parente, Karolini Miranda da Silva, Elton Pedro Nunes Pena, Fabiana Aparecida Cavalcante Silva, Tercilio Calsa Junior, Will de Barros Pita, Marcos Antonio de Morais Jr
Abstract The yeast Dekkera bruxellensis is well-known for its adaptation to industrial ethanol fermentation processes, which can be further improved if nitrate is present in the substrate. To date, the assimilation of nitrate has been considered inefficient because of the apparent energy cost imposed on cell metabolism. Recent research, however, has shown that nitrate promotes growth rate and ethanol
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A recombinant fungal defensin-like peptide-P2 combats Streptococcus dysgalactiae and biofilms Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-03 Qingjuan Zhang, Na Yang, Ruoyu Mao, Ya Hao, Xuanxuan Ma, Da Teng, Huan Fan, Jianhua Wang
Abstract Streptococcus dysgalactiae, considered one of the main pathogens that causes bovine mastitis, is a serious threat to humans and animals. However, the excessive use of antibiotics and the characteristic of S. dysgalactiae forming biofilms in mastitic teat canal have serious clinical implications. In this study, in vivo and in vitro multiple mechanisms of action of P2, a mutant of fungal defensin
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A single nucleotide mutation drastically increases the expression of tumor-homing NGR-TNFα in the E. coli M15-pQE30 system by improving gene transcription Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-02 Jie Chen, Hao Yang, Yanru Feng, Qiuxiao Shi, Zhao Li, Ze Tao, Jie Fan, Youmei Jin, Shengfu Li, Jingqiu Cheng, Xiaofeng Lu
Abstract Due to their potent immune stimulation, tumor necrosis factor alpha (TNFα) variants with tumor-homing activity are attractive as novel antitumor drugs. The promising antitumor effect of NGR-TNFα in clinical trials triggered extensive interest in developing novel tumor-homing TNFα variants in recent years. Owing to its promising antitumor effect, NGR-TNFα is usually used as a control for newly
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Simultaneous quantification of the most common and proteolytic Pseudomonas species in raw milk by multiplex qPCR Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-01 Christopher Maier, Katharina Hofmann, Christopher Huptas, Siegfried Scherer, Mareike Wenning, Genia Lücking
Abstract The heat-stable peptidase AprX, secreted by psychrotolerant Pseudomonas species in raw milk, is a major cause of destabilization and premature spoilage of ultra-high temperature (UHT) milk and milk products. To enable rapid detection and quantification of seven frequent and proteolytic Pseudomonas species (P. proteolytica, P. gessardii, P. lactis, P. fluorescens, P. protegens, P. lundensis
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Harmful effects of metal(loid) oxide nanoparticles Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-01 Eduardo V. Soares, Helena M. V. M. Soares
Abstract The incorporation of nanomaterials (NMs), including metal(loid) oxide (MOx) nanoparticles (NPs), in the most diversified consumer products, has grown enormously in recent decades. Consequently, the contact between humans and these materials increased, as well as their presence in the environment. This fact has raised concerns and uncertainties about the possible risks of NMs to human health
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A bottom-up approach towards a bacterial consortium for the biotechnological conversion of chitin to l -lysine Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-01 Marina Vortmann, Anna K. Stumpf, Elvira Sgobba, Mareike E. Dirks-Hofmeister, Martin Krehenbrink, Volker F. Wendisch, Bodo Philipp, Bruno M. Moerschbacher
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Strategic aromatic residues in the catalytic cleft of the xyloglucanase MtXgh74 modifying thermostability, mode of enzyme action, and viscosity reduction ability Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-02-01 Oksana V. Berezina, Sergey V. Rykov, Angelina K. Polyakova, Marine E. Bozdaganyan, Anna V. Sidochenko, Melanie Baudrexl, Wolfgang H. Schwarz, Vladimir V. Zverlov, Sergey V. Yarotsky
Abstract The thermostable endo-processive xyloglucanase MtXgh74 from Myceliophthora thermophila was used to study the influence of aromatic amino acids in the catalytic cleft on the mode of action and the ability of enzyme to reduce xyloglucan viscosity. The enzyme derivative Mut I with mutations W64A/W67A in the “negative” subsites of the catalytic cleft resulted in a 5.5-fold increase of the Km value
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Targeting effect of berberine on type I fimbriae of Salmonella Typhimurium and its effective inhibition of biofilm Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-26 Chenran Xu, Feiying Wang, Fangfang Huang, Min Yang, Dinggeng He, Le Deng
Abstract As a primary cause of food contamination and human diseases, Salmonella Typhimurium can easily form a biofilm that is difficult to remove from food surfaces, and often causes significant invisible threats to food safety. Although berberine has been widely used as an anti-infective drug in traditional medicine, some basic principles underlying its mechanism, especially the interaction between
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Modulation of the immune response and metabolism in germ-free rats colonized by the probiotic Lactobacillus salivarius LI01 Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-19 Jiafeng Xia, Shiman Jiang, Longxian Lv, Wenrui Wu, Qiangqiang Wang, Qiaomai Xu, Jianzhong Ye, Daiqiong Fang, Yating Li, Jingjing Wu, Xiaoyuan Bian, Liya Yang, Huiyong Jiang, Kaicen Wang, Ren Yan, Lanjuan Li
Abstract The gut microbiota plays an important role in multifaceted physiological functions in the host. Previous studies have assessed the probiotic effects of Lactobacillus salivarius LI01. In this study, we aimed to investigate the potential effects and putative mechanism of L. salivarius LI01 in immune modulation and metabolic regulation through the monocolonization of germ-free (GF) Sprague-Dawley
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Cyclophilin A: a key player for etiological agent infection Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-25 Yating Liao, Dan Luo, Kailan Peng, Yanhua Zeng
Abstract Cyclophilin A (CypA), a key member of the immunophilin family, is the most abundantly expressed isozyme of the 18 known human cyclophilins. Besides acting as an intracellular receptor for cyclosporine A, CypA plays a vital role in microorganismal infections, cardiovascular diseases, liver diseases, kidney diseases, neurodegeneration, cancer, rheumatoid arthritis, periodontitis, sepsis, asthma
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Cancer trigger or remedy: two faces of the human microbiome Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-25 Anna Grenda, Paweł Krawczyk
Currently, increasing attention cancer treatment has focused on molecularly targeted therapies and more recently on immunotherapies targeting immune checkpoints. However, even such advanced treatment may be ineffective. The reasons for this are sought, inter alia, in the human microbiome. In our intestines, there are bacteria that are beneficial to us, but pathogenic microorganisms may also be present
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Bio-based production of poly(3-hydroxybutyrate- co -3-hydroxyvalerate) with modulated monomeric fraction in Escherichia coli Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-23 Dragan Miscevic, Ju-Yi Mao, Bradley Mozell, Kajan Srirangan, Daryoush Abedi, Murray Moo-Young, C. Perry Chou
Abstract In this study, we applied metabolic engineering and bioprocessing strategies to enhance heterologous production of an important biodegradable copolymer, i.e., poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), with a modulated 3-hydroxyvalerate (3-HV) monomeric fraction from structurally unrelated carbon of glycerol in engineered Escherichia coli under different oxygenic conditions. We used
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Surface display of HFBI and DewA hydrophobins on Saccharomyces cerevisiae modifies tolerance to several adverse conditions and biocatalytic performance Appl. Microbiol. Biotechnol. (IF 3.53) Pub Date : 2021-01-23 Cecilia Andreu, Javier Gómez-Peinado, Lex Winandy, Reinhard Fischer, Marcel li del Olmo