
显示样式: 排序: IF: - GO 导出
-
COVID-19 IgG/IgM antibody testing in Los Angeles County, California Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-11-25 Khadije Ahmad, Vahid Rezvanizadeh, Suraj Dahal, April Kinninger, Ahmed K. Ghanem, Suvasini Lakshmanan, Sajad Hamal, Ferdinand Flores, Christopher Dailing, Sion K. Roy, Matthew J. Budoff
Evidence suggests that asymptomatic and mild SARS-CoV-2 infections comprise > 95% of all cases. Developing a test that indicates past infection and possible immunity against the virus is important. We administered 244 antibody tests to three groups of high-risk population. The test consisted of an IgG component and an IgM component. The overall IgM/IgG positivity for patients with none, mild, moderate
-
Practical challenges to the clinical implementation of saliva for SARS-CoV-2 detection Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-11-25 Nancy Matic, Aleksandra Stefanovic, Victor Leung, Tanya Lawson, Gordon Ritchie, Lynne Li, Sylvie Champagne, Marc G. Romney, Christopher F. Lowe
Due to global shortages of flocked nasopharyngeal swabs and appropriate viral transport media during the COVID-19 pandemic, alternate diagnostic specimens for SARS-CoV-2 detection are sought. The accuracy and feasibility of saliva samples collected and transported without specialized collection devices or media were evaluated. Saliva demonstrated good concordance with paired nasopharyngeal swabs for
-
Characteristics of the vaginal microbiomes in prepubertal girls with and without vulvovaginitis Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-16 Wu Xiaoming, Liu Jing, Pan Yuchen, Liu Huili, Zhang Miao, Shu Jing
The present study focused on the characteristics of the vaginal microbiomes in prepubertal girls with and without vulvovaginitis. We collected 24 vaginal samples and 16 fecal samples from 10 girls aged 3–9 years with vulvovaginitis and 16 healthy girls of the same age. The samples were divided into three groups: fecal swabs from healthy controls (HF), vaginal swabs from healthy controls (HVS), and
-
Clinical characteristics of re-hospitalized COVID-19 patients with recurrent positive SARS-CoV-2 RNA: a retrospective study Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-15 Lei Pan, Runsheng Wang, Na Yu, Chao Hu, Junhong Yan, Xiaomin Zhang, Tao Wang, Jungui Hao, Fuquan Gao, Tonggang Liu, Jing Wang, Xiaojing Liu, Mi Mu, Wei Huang, Yongzhong Guo, Changjun Lv, Xiaozhi Wang, Lei Tu, Kun Wan, Guogang Xu
At present, it has been noticed that some patients recovered from COVID-19 present a recurrent positive RNA test of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) after being discharged from hospitals. The purpose of the current study was to characterize the clinical features of re-hospitalized patients with recurrent SARS-CoV-2 positive results. From January 12 to April 1 of 2020, our
-
Retrospective study of group A Streptococcus oropharyngeal infection diagnosis using a rapid antigenic detection test in a paediatric population from the central region of Portugal Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-15 Nuno Mendes, Carmo Miguéis, Jorge Lindo, Teresa Gonçalves, António Miguéis
Group A Streptococcus (GAS) is one of the most important agents of oropharyngeal infection. To avoid unnecessary antibiotic prescription, it is recommended the confirmation of GAS infection in pharyngeal swabs using culture or rapid antigen detection test (RADT). This study aimed to retrospectively analyse the incidence of GAS oropharyngeal infection, detected by RADT, in a paediatric population in
-
C-reactive protein and the neonatal early-onset sepsis calculator for the diagnosis of neonatal sepsis Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-14 Nati Friedman, Sivan Yochpaz, Semyon Zirkin, Jacky Herzlich, Ronella Marom
Our aim was to evaluate the utility of the neonatal early-onset sepsis risk calculator (NEOSC) to the utility of C-reactive protein (CRP) for diagnosing neonatal EOS. This retrospective study reviewed the records of neonates who underwent sepsis workups due to equivocal symptoms and compared their CRP values to the calculator’s recommendations and their cultures. A total of 382 newborns who underwent
-
Applicability and performance of EUCAST’s rapid antimicrobial susceptibility testing (RAST) on primarily sterile body fluids in blood culture bottles in laboratory routine with total lab automation Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-12 Jasmin Kaur Jasuja, Stefan Zimmermann, Irene Burckhardt
Optimisation of microbiological diagnostics in primarily sterile body fluids is required. Our objective was to apply EUCAST’s RAST on primarily sterile body fluids in blood culture bottles with total lab automation (TLA) and to compare results to our reference method Vitek2 in order to report susceptibility results earlier. Positive blood culture bottles (BACTEC™ Aerobic/Anaerobic/PEDS) inoculated
-
A case of infective endocarditis caused by Arthrobacter woluwensis Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-11 Claire Durand, Yanis Kouchit, Laurence Prots, Nicolas Degand, Pierre Dellamonica, Elisa Demonchy, David Chirio
Corynebacteria are rare causative agents of infective endocarditis. This is a reported case of a destructive aorto-mitral infective endocarditis caused by Arthrobacter woluwensis. Microbial identification was achieved by 16S rRNA polymerase chain reaction on valve tissue samples. Outcome was favorable after surgical valve replacement and 4-week antibiotic treatment.
-
Serious adverse events with novel beta-lactam/beta-lactamase inhibitor combinations: a large-scale pharmacovigilance analysis Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-07 Milo Gatti, Emanuel Raschi, Fabrizio De Ponti
The purpose of this study is to characterize adverse events (AEs) of clinical interest reported with ceftolozane-tazobactam and ceftazidime-avibactam, as an aid in monitoring patients affected by severe multidrug-resistant Gram-negative infections. We queried the worldwide FDA Adverse Event Reporting System (FAERS) and performed disproportionality analysis, selecting only designated medical events
-
Brucellosis screening and follow-up of seropositive asymptomatic subjects among household members of shepherds in China Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-07 Jing Hu, Xiangyi Zhang, Huixin Yang, Siwen Zhang, Taijun Wang, Shuqi An, Mujinyan Li, Fande Li, Jingjing Luo, Fangfang Hu, Dali Wang, Shaonan Ni, Jian Sheng, Cui Zhang, Qing Zhen
To measure the seroprevalence of high-exposure populations in brucellosis endemic areas and report the outcome and duration of seropositive asymptomatic subjects, we screened 595 family members of shepherds in Jilin Province, China and then followed up 15 seropositive asymptomatic subjects for 18 months. We found that the seropositive rate of 15.5%. Nearly half of seropositive asymptomatic subjects
-
Diagnosis of SARS-CoV-2 in children: accuracy of nasopharyngeal swab compared to nasopharyngeal aspirate Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-07 Giada Maria Di Pietro, Ester Capecchi, Ester Luconi, Giovanna Lunghi, Samantha Bosis, Giuseppe Bertolozzi, Barbara Cantoni, Giuseppe Marano, Patrizia Boracchi, Elia Biganzoli, Silvana Castaldi, Paola Marchisio
The tests currently used for the identification of SARS-CoV-2 include specimens taken from the upper and lower respiratory tract. Although recommendations from the World Health Organization prioritise the usage of a nasopharyngeal swab (NS), nasopharyngeal aspirates (NPA) are thought to be superior in identifying SARS-CoV-2 in children. To our knowledge, however, no paediatric study has been published
-
Acquisition of multidrug-resistant bacteria and encoding genes among French pilgrims during the 2017 and 2018 Hajj Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-07 Van-Thuan Hoang, Thi-Loi Dao, Tran Duc Anh Ly, Frédérique Gouriet, Linda Hadjadj, Khadidja Belhouchat, Kamel Larbi Chaht, Saber Yezli, Badriah Alotaibi, Didier Raoult, Philippe Parola, Sophie Alexandra Baron, Vincent Pommier de Santi, Jean-Marc Rolain, Philippe Gautret
The objective of this study is to determine the acquisition of multidrug-resistant (MDR) bacteria and antibiotic resistance-encoding genes by French Hajj pilgrims and associated risk factors. Pilgrims traveling during the 2017 and 2018 Hajj were recruited. All pilgrims underwent two successive systematic nasopharyngeal and rectal swabs, pre- and post-Hajj. Specific culture media were used to screen
-
Impact of viral load at admission on the development of respiratory failure in hospitalized patients with SARS-CoV-2 infection Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-07 Cristina de la Calle, Antonio Lalueza, Mikel Mancheño-Losa, Guillermo Maestro-de la Calle, Jaime Lora-Tamayo, Estibaliz Arrieta, Ana García-Reyne, Irene Losada, Borja de Miguel, Raquel Díaz-Simón, Francisco López-Medrano, Mario Fernández-Ruiz, Octavio Carretero, Rafael San Juan, José María Aguado, Carlos Lumbreras
The aim of our study was to elucidate if SARS-CoV-2 viral load on admission, measured by real-time reverse transcriptase–polymerase chain reaction (rRT-PCR) cycle threshold (Ct) value on nasopharyngeal samples, was a marker of disease severity. All hospitalized adult patients with a diagnosis of SARS-CoV-2 infection by rRT-PCR performed on a nasopharingeal sample from March 1 to March 18 in our institution
-
Epidemiological, molecular, and clinical features of rotavirus infections among pediatrics in Qatar Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-07 Shilu Mathew, Khalid Al Ansari, Asmaa A. Al Thani, Hassan Zaraket, Hadi M. Yassine
Acute gastroenteritis (AGE) remains a major cause of diarrhea in developing and developed countries. Rotavirus (RV) is a leading cause of severe pediatric diarrhea worldwide. Here we report on the prevalence of circulating genotypes in association with demographics and clinical manifestations outcomes in Qatar. A total of 231 RV-positive fecal samples were collected from children suffering from AGE
-
Characteristics and outcome according to underlying disease in non-AIDS patients with acute respiratory failure due to Pneumocystis pneumonia Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-07 Gaston Burghi, Lucie Biard, Antoine Roux, Sandrine Valade, Florence Robert-Gangneux, Samia Hamane, Daniéle Maubon, Anne Debourgogne, Soléne Le Gal, Fréderic Dalle, Marion Leterrier, Dominique Toubas, Christelle Pomares, Anne Pauline Bellanger, Julie Bonhomme, Antoine Berry, Xavier Iriart, Isabelle Durand-Joly, Denis Magne, Denis Pons, Christophe Hennequin, Eric Maury, Elie Azoulay, Virginie Lemiale
In the non-AIDS group, several underlying conditions and immune defects could lead to different PCP presentations. This study compared PCP presentation and outcome according to the underlying disease. A secondary analysis of a previously published prospective observational study including 544 PCP patients was done. Only non-AIDS patients were included. Underlying disease was defined as chronic lymphocytic
-
Performance of VITEK 2, E-test, Kirby–Bauer disk diffusion, and modified Kirby–Bauer disk diffusion compared to reference broth microdilution for testing tigecycline susceptibility of carbapenem-resistant K. pneumoniae and A. baumannii in a multicenter study in China Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-07 Dandan Yin, Yan Guo, Min Li, Wenjuan Wu, Jin Tang, Ying Liu, Feng Chen, Yuxing Ni, Jingyong Sun, Hong Zhang, Hu Zhao, Fupin Hu
Tigecycline is an alternative antibiotic for managing carbapenem-resistant Gram-negative bacterial infections. However, disk diffusion and automated testing often show false-intermediate or false-resistant results in tigecycline susceptibility, misleading clinical antimicrobial therapy. Broth microdilution (BMD) is the reference method for testing tigecycline susceptibility, but it is labor intensive
-
Serodiagnosis of Lyme borreliosis—is IgM in serum more harmful than helpful? Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-07 Henrik Hillerdal, Anna J. Henningsson
Interpretation of serological findings in suspected Lyme borreliosis (LB) may be challenging and IgM reactivities in serum are often unspecific (false positive). There is a risk for overdiagnosis of LB, inadequate use of antibiotics, and potential delay of proper diagnosis. In this study, we evaluated the diagnostic value of IgM analysis in serum and IgM antibody index (AI) in LB diagnosis. This was
-
Impact of surgical timing on survival in patients with infective endocarditis: a time-dependent analysis Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-07 Marco Ripa, Stefania Chiappetta, Barbara Castiglioni, Eustachio Agricola, Elena Busnardo, Silvia Carletti, Alessandro Castiglioni, Michele De Bonis, Giovanni La Canna, Chiara Oltolini, Ursola Pajoro, Renée Pasciuta, Chiara Tassan Din, Paolo Scarpellini
The purpose of this study was to evaluate the impact of surgical timing on survival in patients with left-sided infective endocarditis (IE). This was a retrospective study including 313 patients with left-sided IE between 2009 and 2017. Surgery was defined as urgent (US) or early (ES) if performed within 7 or 28 days, respectively. A multivariable Cox regression analysis including US and ES as time-dependent
-
Inference of SARS-CoV-2 spike-binding neutralizing antibody titers in sera from hospitalized COVID-19 patients by using commercial enzyme and chemiluminescent immunoassays Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-06 Arantxa Valdivia, Ignacio Torres, Víctor Latorre, Clara Francés-Gómez, Eliseo Albert, Roberto Gozalbo-Rovira, María Jesús Alcaraz, Javier Buesa, Jesús Rodríguez-Díaz, Ron Geller, David Navarro
Whether antibody levels measured by commercially available enzyme or chemiluminescent immunoassays targeting the SARS-CoV-2 spike (S) protein can act as a proxy for serum neutralizing activity remains to be established for many of these assays. We evaluated the degree of correlation between neutralizing antibodies (NtAb) binding the SARS-CoV-2 spike (S) protein and SARS-CoV-2-S-IgG levels measured
-
Secular trends in the epidemiology and clinical characteristics of Enterococcus faecalis infective endocarditis at a referral center (2007–2018) Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-06 Laura Escolà-Vergé, Nuria Fernández-Hidalgo, María Nieves Larrosa, Ruben Fernandez-Galera, Benito Almirante
The aim of the study was to analyze the epidemiological and clinical changes in EFIE. All definite IE episodes treated at a referral center between 2007 and 2018 were registered prospectively, and a trend test was used to study etiologies over time. EFIE cases were divided into three periods, and clinical differences between them were analyzed. All episodes of E. faecalis monomicrobial bacteremia (EFMB)
-
Antibiotic susceptibility and molecular analysis of invasive Neisseria meningitidis recovered in the Republic of Ireland, 1996 to 2016 Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-05 Désirée E. Bennett, K. L. Meyler, M. T. Cafferkey, R. J. Cunney
This study examined the antimicrobial susceptibility of invasive meningococcal disease (IMD)–associated Neisseria meningitidis recovered in the Republic of Ireland between 1996 and 2016. In total, 1359 isolates representing over one-third of all laboratory-confirmed cases of IMD diagnosed each epidemiological year (EY; July 1–June 30) were analysed. All isolates were susceptible to ciprofloxacin, rifampicin
-
Tigecycline antibacterial activity, clinical effectiveness, and mechanisms and epidemiology of resistance: narrative review Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-05 Sajad Yaghoubi, Angelina Olegovna Zekiy, Marcela Krutova, Mehrdad Gholami, Ebrahim Kouhsari, Mohammad Sholeh, Zahra Ghafouri, Farajolah Maleki
Tigecycline is unique glycylcycline class of semisynthetic antimicrobial agents developed for the treatment of polymicrobial infections caused by multidrug-resistant Gram-positive and Gram-negative pathogens. Tigecycline evades the main tetracycline resistance genetic mechanisms, such as tetracycline-specific efflux pump acquisition and ribosomal protection, via the addition of a glycyclamide moiety
-
Deep learning model for prediction of extended-spectrum beta-lactamase (ESBL) production in community-onset Enterobacteriaceae bacteraemia from a high ESBL prevalence multi-centre cohort Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-05 Alfred Lok Hang Lee, Curtis Chun Kit To, Angus Lang Sun Lee, Ronald Cheong Kin Chan, Janus Siu Him Wong, Chun Wai Wong, Viola Chi Ying Chow, Raymond Wai Man Lai
Adequate empirical antimicrobial coverage is instrumental in clinical management of community-onset Enterobacteriaceae bacteraemia in areas with high ESBL prevalence, while balancing the risk of carbapenem overuse and emergence of carbapenem-resistant organisms. It is unknown whether machine learning offers additional advantages to conventional statistical methods in prediction of ESBL production.
-
Quantitative real-time PCR in Borrelia persica tick-borne relapsing fever demonstrates correlation with the Jarisch-Herxheimer reaction Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-04 Adin Breuer, Orli Megged, Livnat Kashat, Marc Victor Assous
The purpose of this study is to explore whether a correlation exists between the bacterial load of Borrelia persica in tick-borne relapsing fever (TBRF), established by quantitative real-time PCR, and the development of Jarisch-Herxheimer reaction (JHR) after the initiation of antibiotic treatment. Forty-two blood samples were included in our study. The mean bacterial load, as established by real-time
-
Correction to: Factors affecting the discharge of patients from hospital with seasonal influenza—the role of the Rapid Influenza testing in hospital discharges Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-11-25 Ruchir Chavada, Liam Clifford, Owen Weisback
A Correction to this paper has been published: https://doi.org/10.1007/s10096-020-04105-1
-
Stewardship program on carbapenem prescriptions in a tertiary hospital for adults and children in France: a cohort study Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-03 Julie Poline, Martine Postaire, Perrine Parize, Benoit Pilmis, Emmanuelle Bille, Jean Ralph Zahar, Pierre Frange, Jérémie F. Cohen, Olivier Lortholary, Julie Toubiana
Antimicrobial stewardship programs aim at reducing the overuse of broad-spectrum antibiotics such as carbapenems, but their impact remains unclear. We compared the use of carbapenems between paediatric and adult subjects admitted to a French tertiary hospital and described the intervention of an antibiotic stewardship team (AST). As part of AST routine activity, all adult and paediatric patients receiving
-
Forgoing transesophageal echocardiogram in selected patients with complicated Staphylococcus aureus bacteremia Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-03 John C. Lam, Daniel B. Gregson, Ranjani Somayaji, Stephen Robinson, John M. Conly, Lisa Welikovitch, Michael D. Parkins
Infective endocarditis (IE) has been increasingly recognized as an important complication of Staphylococcus aureus bacteremia (SAB), leading to a low threshold for echocardiography and extended treatment with anti-staphylococcal agents. However, outside of IE, many indications for prolonged anti-staphylococcal therapy courses are present. We sought to determine the frequency in which findings from
-
Clinical performance of the H. PYLORI QUIK CHEK™ and H. PYLORI CHEK™ assays, novel stool antigen tests for diagnosis of Helicobacter pylori Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-03 Magnus Halland, Rashidul Haque, Jost Langhorst, James H. Boone, William A. Petri
Infection with Helicobacter pylori is a global health issue, and rapid and accurate testing is a key to diagnosis. We aimed to assess the performance of two novel enzyme immunoassays (EIA), the H. PYLORI QUIK CHEK™ and the H. PYLORI CHEK™ assays, for the detection of H. pylori antigen in stool. Patients from five geographically diverse sites across the USA, Germany, and in Bangladesh were tested for
-
Antibiotic resistance profile and molecular characterization of Staphylococcus aureus strains isolated in hospitals in Kabul, Afghanistan Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-03 Haji Mohammad Naimi, Camille André, Michèle Bes, Anne Tristan, Claude-Alexandre Gustave, François Vandenesch, Qand Agha Nazari, Frédéric Laurent, Céline Dupieux
The aim of this study was to investigate the molecular features and the antibiotic resistance profile of 98 clinical Staphylococcus aureus isolates collected during 6 months in two hospitals of Kabul, Afghanistan. For all isolates, antimicrobial resistance patterns were determined by the disc diffusion method (including methicillin resistance which was detected using cefoxitin). The presence of the
-
Nosocomial infections associated to COVID-19 in the intensive care unit: clinical characteristics and outcome Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-03 Tommaso Bardi, Vicente Pintado, Maria Gomez-Rojo, Rosa Escudero-Sanchez, Amal Azzam Lopez, Yolanda Diez-Remesal, Nilda Martinez Castro, Patricia Ruiz-Garbajosa, David Pestaña
Bacterial and fungal co-infection has been reported in patients with COVID-19, but there is limited experience on these infections in critically ill patients. The objective of this study was to assess the characteristics and ouctome of ICU-acquired infections in COVID-19 patients. We conducted a retrospective single-centre, case-control study including 140 patients with severe COVID-19 admitted to
-
Streptococcus dysgalactiae subsp. equisimilis from invasive and non-invasive infections in Spain: combining epidemiology, molecular characterization, and genetic diversity Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-03 Beatriz Rojo-Bezares, Laura Toca, José Manuel Azcona-Gutiérrez, Nerea Ortega-Unanue, Paula Toledano, Yolanda Sáenz
The purpose of this study was to characterize the antibiotic resistance, virulence, and genetic diversity among invasive and non-invasive Streptococcus dysgalactiae subsp. equisimilis (SDSE) isolates. SDSE were isolated from clinical samples of outpatients and inpatients cares in La Rioja region (Spain) during 2012–2015. The analyses performed were susceptibility testing by disc diffusion, resistance
-
COVID-19: angiotensin-converting enzyme 2 (ACE2) expression and tissue susceptibility to SARS-CoV-2 infection Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-03 Stephany Beyerstedt, Expedito Barbosa Casaro, Érika Bevilaqua Rangel
COVID-19 pandemic is caused by the novel coronavirus SARS-CoV-2. Angiotensin-converting enzyme 2 (ACE2) is not only an enzyme but also a functional receptor on cell surfaces through which SARS-CoV-2 enters the host cells and is highly expressed in the heart, kidneys, and lungs and shed into the plasma. ACE2 is a key regulator of the renin–angiotensin–aldosterone system (RAAS). SARS-CoV-2 causes ACE/ACE2
-
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-02 Nathalie Wurtz, Gwilherm Penant, Priscilla Jardot, Nathalie Duclos, Bernard La Scola
The emergence of COVID-19 disease due to SARS-CoV-2 at the end of 2019 was rapidly associated with the isolation of the strain from co-culture onto VERO cells. These isolations quickly made it possible to carry out the first tests for antiviral agents’ susceptibility and drug repurposing. However, it seems important to make an inventory of all the cells that can support the growth of this virus and
-
Follow-up blood cultures in E. coli and Klebsiella spp. bacteremia—opportunities for diagnostic and antimicrobial stewardship Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-02 Jeannie D. Chan, Ashley Ta, John B. Lynch, Chloe Bryson-Cahn
Uncomplicated Enterobacteriaceae bacteremia is usually transient and may not require follow-up blood cultures (FUBC). This is a retrospective observational study conducted at a university-affiliated urban teaching hospital in Seattle, WA. All patients ≥ 18 years hospitalized between July 2014 and August 2019 with ≥ 1 positive blood culture for either Escherichia coli or Klebsiella species were included
-
Comparison of blood cultures versus T2 Candida Panel in management of candidemia at a large community hospital Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-02 Taylor D. Steuber, Lauren Butler, Adam Sawyer, Rachel Chappell, Jonathan Edwards
The T2 Candida Panel (T2CP) has high sensitivity and specificity to detect candidemia. Its role in the diagnosis and management of candidemia compared to blood cultures (BC) remains unclear. The purpose of this study was to evaluate the T2CP versus BC in detecting and treating candidemia. A retrospective, observational cohort study was conducted to compare clinical outcomes in patients with candidemia
-
Lyme neuroborreliosis in Swedish children—PCR as a complementary diagnostic method for detection of Borrelia burgdorferi sensu lato in cerebrospinal fluid Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2021-01-02 Barbro H. Skogman, Peter Wilhelmsson, Stephanie Atallah, Ann-Cathrine Petersson, Katarina Ornstein, Per-Eric Lindgren
The aim of this study was to evaluate polymerase chain reaction (PCR) as a diagnostic method for the detection of Borrelia burgdorferi s.l. in CSF of Swedish children with LNB. This study was performed retrospectively on CSF and serum samples collected from children evaluated for LNB (n = 233) and controls with other specific neurological disorders (n = 59) in a Swedish Lyme endemic area. For anti-Borrelia
-
Endocarditis due to Staphylococcus lugdunensis —a retrospective national registry–based study Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-12-30 Malin Hagstrand Aldman, Magnus Rasmussen, Lars Olaison, Lisa I. Påhlman
We present characteristics of infective endocarditis (IE) caused by Staphylococcus lugdunensis and compare with IE caused by Staphylococcus aureus and other CoNS, in the National Swedish Registry of IE (2008–2018). Thirty episodes of S. lugdunensis IE were registered, of which 21 cases affected native valves, and 7 patients were subjected to surgery. The mortality rate at 30 days was significantly
-
Unexpected Mycoplasma hominis infection in two renal transplant recipients traced back to the same donor by whole-genome sequencing Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-12-23 V. Hinić, H. M. B. Seth-Smith, S. Damm, P. Amico, N. Khanna, A. Egli, V. Bättig
Mycoplasma hominis is a common colonizer of the lower genitourinary tract. Although its clinical relevance for causing urogenital infections in immunocompetent individuals is controversial, this bacterium has been involved in severe invasive infections in allograft recipients. In this report, we describe two cases of M. hominis infection in two young renal transplant recipients within the first month
-
Differentiating influenza from COVID-19 in patients presenting with suspected sepsis Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-12-03 Valentino D’Onofrio, Eveline Van Steenkiste, Agnes Meersman, Luc Waumans, Reinoud Cartuyvels, Karlijn Van Halem, Peter Messiaen, Inge C. Gyssens
There is a need for a quick assessment of severely ill patients presenting to the hospital. The objectives of this study were to identify clinical, laboratory and imaging parameters that could differentiate between influenza and COVID-19 and to assess the frequency and impact of early bacterial co-infection. A prospective observational cohort study was performed between February 2019 and April 2020
-
Correction to: Serotype distribution of Streptococcus pneumoniae among healthy carriers and clinical patients: a systematic review from Iran Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-10-29 Maryam Alizadeh Chamkhaleh, Abdoulreza Esteghamati, Shirin Sayyahfar, Alireza Gandomi-Mohammadabadi, Javad Balasi, Hossein Abdiaei, Yousef Moradi, Maziar Moradi-Lakeh
A correction to this paper has been published: https://doi.org/10.1007/s10096-020-04084-3.
-
Whole genome sequencing analysis of multidrug-resistant tuberculosis in Singapore, 2006–2018 Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-11-15 Cynthia B. E. Chee, Leo K. Y. Lim, Rick T. H. Ong, Li Hwei Sng, Li Yang Hsu, Vernon J. M. Lee, Yee Tang Wang
There were 290 multidrug-resistant (MDR)-TB cases diagnosed in Singapore from 2006 to 2018. Eighty-one percent were foreign-born. Spoligotyping and MIRU-VNTR methods identified 108 patients in 24 clusters. The Beijing spoligotype accounted for 22 clusters. Whole genome sequencing (WGS) analysis reduced the number of clustered patients and clusters to 43 and nine respectively. One MIRU cluster was redefined
-
Epidemiological characteristics of severe fever with thrombocytopenia syndrome in Hefei of Anhui Province: a population-based surveillance study from 2011 to 2018 Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-11-13 Enqing You, Ling Wang, Lei Zhang, Jinju Wu, Kefu Zhao, Fen Huang
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne disease caused by the SFTS virus (SFTSV) of family Phenuiviridae. Since the virus was first identified in 2009, it has been rapidly spread in many Asian countries including Myanmar, Korea, Japan, and central China. In this study, we investigated the epidemiological characteristics of SFTS patients in Hefei from 2011 to 2018
-
Characteristics of gram-negative bacteremia during febrile neutropenia among allogeneic hematopoietic stem cell transplant recipients on levofloxacin prophylaxis Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-11-13 Sho Ogura, Muneyoshi Kimura, Shinsuke Takagi, Takashi Mitsuki, Mitsuhiro Yuasa, Kosei Kageyama, Daisuke Kaji, Aya Nishida, Yuki Taya, Kazuya Ishiwata, Hisashi Yamamoto, Yuki Asano-Mori, Go Yamamoto, Naoyuki Uchida, Atsushi Wake, Shuichi Taniguchi, Hideki Araoka
The aim of this study is to clarify the characteristics of gram-negative bacteremia (GNB), including extended-spectrum β-lactamase (ESBL)-producing pathogens, among allogeneic hematopoietic stem cell transplant (allo-HSCT) recipients on levofloxacin (LVFX) prophylaxis. A retrospective analysis on GNB at the first episode of febrile neutropenia (FN) was conducted among allo-HSCT recipients (age ≥ 20 years)
-
Whole genome sequencing to decipher the virulence phenotype of hypervirulent Klebsiella pneumoniae responsible for liver abscess, Marseille, France Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-11-12 Sophie Alexandra Baron, Léa-Marie Pascale, Matthieu Million, Antoine Briantais, Jean-Marc Durand, Linda Hadjadj, Jean-Marc Rolain
We described three clinical cases of pyogenic liver abscess caused by hypervirulent Klebsiella pneumoniae (hvKp) successfully treated by prolonged antibiotherapy, in which one case was complicated by endophthalmitis. Whole genome sequencing helped to confirm the diagnosis of these hvKp strains, which belong to clonal complexes CC86 and CC23 and carried hvKp-associated genes (magA and/or rmpA). This
-
Development and evaluation of a serological test for diagnosis of COVID-19 with selected recombinant spike proteins Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-11-12 Pingping Zhang, Qi Gao, Tang Wang, Yuehua Ke, Fei Mo, Ruizhong Jia, Wanbing Liu, Lei Liu, Shangen Zheng, Yuzhen Liu, Luping Li, Yao Wang, Lei Xu, Kun Hao, Wei Min, Xiaoli Liu, Ruifu Yang, Shiyue Li, Changqing Lin, Yong Zhao
Serological test is a valuable diagnostic tool for coronavirus disease 2019 (COVID-19). However, considerable improvements to these tests are needed, especially in the detection sensitivity. In this study, six recombinant nucleocapsid and spike proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were prepared and evaluated, including three prokaryotic expression nucleocapsid proteins
-
Evaluating the serological status of COVID-19 patients using an indirect immunofluorescent assay, France Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-11-11 S. Edouard, P. Colson, C. Melenotte, F. Di Pinto, L. Thomas, B. La Scola, M. Million, H. Tissot-Dupont, P. Gautret, A. Stein, P. Brouqui, P. Parola, J.-C. Lagier, D. Raoult, Michel Drancourt
An indirect in-house immunofluorescent assay was developed in order to assess the serological status of COVID-19 patients in Marseille, France. Performance of IFA was compared to a commercial ELISA IgG kit. We tested 888 RT-qPCR-confirmed COVID-19 patients (1302 serum samples) and 350 controls including 200 sera collected before the pandemic, 64 sera known to be associated with nonspecific serological
-
Concomitant emphysema might increase the false-negative rate of urinary antigen tests in patients with pneumococcal pneumonia: results from a retrospective study Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-11-04 Erika Kobayashi, Kakuhiro Yamaguchi, Rie Nagaoka, Shinjiro Sakamoto, Yasushi Horimasu, Takeshi Masuda, Shintaro Miyamoto, Taku Nakashima, Hiroshi Iwamoto, Kazunori Fujitaka, Michiya Yokozaki, Hiroki Ohge, Hironobu Hamada, Noboru Hattori
The urinary antigen test (UAT) is a rapid diagnostic method for pneumococcal pneumonia, but the high false-negative rate of 30% may affect its reliability. To maximize the utility of UAT, it is necessary to investigate the patient factors affecting UAT results. However, there is no report elucidating the association between its utility and pre-existing lung abnormalities. We retrospectively reviewed
-
Rates of bacterial co-infections and antimicrobial use in COVID-19 patients: a retrospective cohort study in light of antibiotic stewardship Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-11-02 Kathrin Rothe, Susanne Feihl, Jochen Schneider, Fabian Wallnöfer, Milena Wurst, Marina Lukas, Matthias Treiber, Tobias Lahmer, Markus Heim, Michael Dommasch, Birgit Waschulzik, Alexander Zink, Christiane Querbach, Dirk H. Busch, Roland M. Schmid, Gerhard Schneider, Christoph D. Spinner
The coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread worldwide. Bacterial co-infections are associated with unfavourable outcomes in respiratory viral infections; however, microbiological and antibiotic data related to COVID-19 are sparse. Adequate use of antibiotics in line with antibiotic stewardship (ABS) principles is warranted
-
Laboratory evaluation of the Sigma Transwab® transport system for the recovery of Candida species using the Clinical and Laboratory Standards Institute (CLSI) document M40-A2 Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-10-13 E. Elcocks, E.C. Adukwu
Rising healthcare complications due to fungal infections increase the importance of efficient specimen collection and maintenance systems for correct identification and diagnosis. The CLSI M40-A2 protocol provides guidelines for laboratories assessing quality of medical transport devices, including swab transport systems (STS). This study assessed the efficiency of the Sigma Transwab® foam and flock
-
The clinical presentation, treatment and outcome of serologically confirmed paediatric Lyme disease in the Republic of Ireland over a 5-year period: a retrospective cohort study Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-10-11 Karina M. Forde, Joanne O’Gorman, Patrick J. Gavin, Matthew S. Dryden, Deirbhile Keady, Belinda Hanahoe, Colm McDonnell, Lorraine Power, Bartley Cryan, James Sweeney, Karl F. Conyard, Michael J. O’Grady
Lyme disease (LD) is the most common tick-borne illness in Europe. Population-based studies in European children are few. This study aimed to assess the incidence, clinical presentation, treatment and outcome of serologically confirmed paediatric LD in the Republic of Ireland over a 5-year period. A retrospective review of records from accredited laboratories performing Borrelia burgdorferi serological
-
Is recurrence possible in coronavirus disease 2019 (COVID-19)? Case series and systematic review of literature Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-10-10 Anna Gidari, Marco Nofri, Luca Saccarelli, Sabrina Bastianelli, Samuele Sabbatini, Silvia Bozza, Barbara Camilloni, Igino Fusco-Moffa, Claudia Monari, Edoardo De Robertis, Antonella Mencacci, Daniela Francisci
Can a patient diagnosed with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) be infected again? This question is still unsolved. We tried to analyze local and literature cases with a positive respiratory swab after recovery. We collected data from symptomatic patients diagnosed with SARS-CoV-2 infection in the Italian Umbria Region that, after recovery, were again positive for SARS-CoV-2
-
Effect of delayed entry on performance of the BACT/ALERT FAN PLUS bottles in the BACT/ALERT VIRTUO blood culture system Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-10-09 Mary Adamik, Anne Hutchins, Jasmin Mangilit, Betsy Katzin, Heather Totty, Parampal Deol
Delayed entry of patient blood culture samples into a microbial detection system is unavoidable at times, due to off-shift staffing or transporting samples to centralized laboratories. Pre-incubation time and temperature of blood culture bottles are the most critical factors impacting recovery and detection of microorganisms. A total of 1377 BACT/ALERT® (BTA) Fastidious Antimicrobial Neutralization
-
Drug screening and development from the affinity of S protein of new coronavirus with ACE2 Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-10-09 Yue-Peng Jiang, Xiao-Xuan Zhao, Hui-Qing Lv, Cheng-Ping Wen
Recently, various studies have shown that angiotensin-converting enzyme 2 (ACE2) acts as the “doorknob” that can be bound by the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which conduces to its entrance to the host cells, and plays an important role in corona virus disease 2019 (COVID-19). This paper aims to collect and sorts out the existing drugs, which exert the
-
Study on risk factors, bacterial species, and drug resistance of acute pyelonephritis associated with ureteral stent after percutaneous nephrolithotomy Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-10-09 Guo Jiang, Jiang Li, He Long, Chen Qiulin, Ren Jin, Yang Yaodong, Dong Xingyou, Zhao Jiang, Zhang Zhenyang
The purpose of this study is to explore the risk factors, bacterial species, and drug resistance of acute pyelonephritis (AP) associated with ureteral stent after percutaneous nephrolithotomy (PCNL) and to provide reference for clinical intervention. The clinical data of 415 patients with indwelling ureteral stent after PCNL from December 2016 to May 2019 were analyzed retrospectively. The patients
-
Prevalence of hyposmia and hypogeusia in 390 COVID-19 hospitalized patients and outpatients: a cross-sectional study Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-10-08 Agathe Nouchi, Julie Chastang, Makoto Miyara, Julie Lejeune, André Soares, Gladys Ibanez, David Saadoun, Capucine Morélot-Panzini, Thomas Similowski, Zahir Amoura, Jacques Boddaert, Eric Caumes, Alexandre Bleibtreu, Alain Lorenzo, Florence Tubach, Valérie Pourcher
Anecdotal evidence rapidly accumulated during March 2020 from sites around the world that sudden hyposmia and hypogeusia are significant symptoms associated with the SARS-CoV-2 pandemic. Our objective was to describe the prevalence of hyposmia and hypogeusia and compare it in hospitalized and non-hospitalized COVID-19 patients to evaluate an association of these symptoms with disease severity. We performed
-
Comparison of conventional molecular and whole-genome sequencing methods for subtyping Salmonella enterica serovar Enteritidis strains from Tunisia Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-10-08 Boutheina Ksibi, Sonia Ktari, Houcemeddine Othman, Kais Ghedira, Sonda Maalej, Basma Mnif, Mohamed salah Abbassi, Laetitia Fabre, Faouzia Rhimi, Simon Le Hello, Adnene Hammami
We sought to determine the relative value of conventional molecular methods and whole-genome sequencing (WGS) for subtyping Salmonella enterica serovar Enteritidis recovered from 2000 to 2015 in Tunisia and to investigate the genetic diversity of this serotype. A total of 175 Salmonella Enteritidis isolates were recovered from human, animal, and foodborne outbreak samples. Pulsed-field gel electrophoresis
-
Chronic hepatitis B virus infection in Italy during the twenty-first century: an updated survey in 2019 Eur. J. Clin. Microbiol. Infect. Dis. (IF 2.837) Pub Date : 2020-10-08 Tommaso Stroffolini, Alessia Ciancio, Caterina Furlan, Maria Vinci, Grazia Anna Niro, Maurizio Russello, Guido Colloredo, Filomena Morisco, Nicola Coppola, Sergio Babudieri, Luigina Ferrigno, Caterina Sagnelli, Evangelista Sagnelli
The aim of this study is to provide updates on the characteristics of chronic HBsAg carriers in Italy before the advent of new drugs eliminating or functionally inactivating the genome HBV reservoirs. HBV endemicity has greatly decreased in Italy over the past decades. A not negligible number of chronic HBsAg carriers are still alive in the country. Chronic HBsAg carriers consecutively referring to