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Unlocking bacterial defense: Exploring the potent inhibition of NorA efflux pump by coumarin derivatives in Staphylococcus aureus Microb. Pathog. (IF 3.8) Pub Date : 2024-03-17 Ana Luíza A.R. Martin, Raimundo Luiz Silva Pereira, Janaína Esmeraldo Rocha, Pablo A.M. Farias, Thiago S. Freitas, Francisco Rodrigo de Lemos Caldas, Fernando G. Figueredo, Nadghia Figueiredo Leite Sampaio, Cícera Datiane de Morais Oliveira-Tintino, Saulo Relison Tintino, Gabriel Costa A. da Hora, Maria Carolina Pacheco Lima, Irwin Rose de A. Menezes, Diogo T. Carvalho, Henrique D.M. Coutinho, Marta
The occurrence of bacterial resistance has been increasing, compromising the treatment of various infections. The high virulence of allows for the maintenance of the infectious process, causing many deaths and hospitalizations. The MepA and NorA efflux pumps are transporter proteins responsible for expelling antimicrobial agents such as fluoroquinolones from the bacterial cell. Coumarins are phenolic
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Application of cuminaldehyde and ciprofloxacin for the effective control of biofilm assembly of Pseudomonas aeruginosa: A combinatorial study Microb. Pathog. (IF 3.8) Pub Date : 2024-03-16 Moumita Malik, Sharmistha Das, Poulomi Chakraborty, Payel Paul, Ritwik Roy, Anirban Das Gupta, Sarita Sarkar, Sudipta Chatterjee, Alakesh Maity, Monikankana Dasgupta, Ranojit Kumar Sarker, Prosun Tribedi
is widely associated with biofilm-mediated antibiotic resistant chronic and acute infections which constitute a persistent healthcare challenges. Addressing this threat requires exploration of novel therapeutic strategies involving the combination of natural compounds and conventional antibiotics. Hence, our study has focused on two compounds; cuminaldehyde and ciprofloxacin, which were strategically
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Good girl goes bad: Understanding how gut commensals cause disease Microb. Pathog. (IF 3.8) Pub Date : 2024-03-16 Priyankar Dey
This review examines the complex connection between commensal microbiota and the development of opportunistic infections. Several underlying conditions, such as metabolic diseases and weakened immune systems, increase the vulnerability of patients to opportunistic infections. The increasing antibiotic resistance adds significant complexity to the management of infectious diseases. Although commensals
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Antagonistic activity and mechanism of Bacillus subtilis CG-6 suppression of root rot and growth promotion in Alfalfa Microb. Pathog. (IF 3.8) Pub Date : 2024-03-15 Jiaxin Chen, Run Cai, Lu Tang, Dan Wang, Ruiwei Lv, Changhong Guo
Root rot is a common disease, that severely affects the yield and quality of alfalfa. Biocontrol is widely used to control plant diseases caused by pathogenic fungi, however, biocontrol strains for alfalfa root rot are very limited. In this study, a CG-6 strain with a significant biocontrol effect on alfalfa root rot was isolated. CG-6 secretes antibacterial enzymes and siderophore, phosphate solubilization
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Acanthopanax senticosus cultures fermented by Lactobacillus rhamnosus enhanced immune response through improvement of antioxidant activity and inflammation in crucian carp (Carassius auratus) Microb. Pathog. (IF 3.8) Pub Date : 2024-03-15 Yi-Han Ma, Yu-Di Sheng, Di Zhang, Jun-Tong Liu, Ye Tian, Hui Li, Xiao-Fei Li, Na Li, Peng Sun, Shahrood Ahmed Siddiqui, Wu-wen Sun, Lei Zhang, Xiao-Feng Shan, Chun-Feng Wang, Ai-Dong Qian, Dong-Xing Zhang
Lactic acid bacteria (LAB) have been recognized as safe microorganism that improve micro-flora disturbances and enhance immune response. A well-know traditional herbal medicine, (As) was extensively utilized in aquaculture to improve growth performance and disease resistance. Particularly, the septicemia, skin wound and gastroenteritis caused by threaten the health of aquatic animals and human. However
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Enhanced control efficacy of Bacillus subtilis NM4 via integration of chlorothalonil on potato early blight caused by Alternaria solani Microb. Pathog. (IF 3.8) Pub Date : 2024-03-14 Jun Su Noh, Seo Hyun Hwang, Chaw Ei Htwe Maung, Jeong-Yong Cho, Kil Yong Kim
Early blight caused by is a common foliar disease of potato around the world, and serious infections result in reduced yields and marketability due to infected tubers. The major aim of this study is to figure out the synergistic effect between microorganism and fungicides and to evaluate the effectiveness of NM4 in the control of early blight in potato. Based on its colonial morphology and a 16S rRNA
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Macrophage immunophenotypes in Jorge Lobo’s disease and lepromatous leprosy- A comparative study Microb. Pathog. (IF 3.8) Pub Date : 2024-03-13 Bruna Morassi Sasso, Andrelou Fralete Ayres Vallarelli, Patricia Sammarco Rosa, Andrea de Faria Fernandes Belone, Paulo Eduardo Neves Ferreira Velho, Maria Leticia Cintra
Jorge Lobo's disease (JLD) and lepromatous leprosy (LL) share several clinical, histological and immunological features, especially a deficiency in the cellular immune response. Macrophages participate in innate and adaptive inflammatory immune responses, as well as in tissue regeneration and repair. Macrophage function deficiency results in maintenance of diseases. M1 macrophages produce pro-inflammatory
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Biological activity of silver nanoparticles synthesized using viticultural waste Microb. Pathog. (IF 3.8) Pub Date : 2024-03-12 Anna Miškovská, Jana Michailidu, Irena Jarošová Kolouchová, Ludovica Barone, Rosalba Gornati, Aurora Montali, Gianluca Tettamanti, Francesca Berini, Flavia Marinelli, Jan Masák, Alena Čejková, Olga Maťátková
This research paper presents a novel approach to the green synthesis of silver nanoparticles (AgNPs) using viticultural waste, allowing to obtain NP dispersions with distinct properties and morphologies (monodisperse and polydisperse AgNPs, referred to as mAgNPs and pAgNPs) and to compare their biological activities. Our synthesis method utilized the ethanolic extract of pruning residues, resulting
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Isolation and characterization of a G9P[23] porcine rotavirus strain AHFY2022 in China Microb. Pathog. (IF 3.8) Pub Date : 2024-03-11 Jianxin Wang, Jinzhu Zhou, Xuejiao Zhu, Xianyu Bian, Nan Han, Baochao Fan, Laqiang Gu, Xi Cheng, Sufen Li, Ran Tao, Jizong Li, Xuehan Zhang, Bin Li
Rotavirus group A (RVA) is a main pathogen causing diarrheal diseases in humans and animals. Various genotypes are prevalent in the Chinese pig herd. The genetic diversity of RVA lead to distinctly characteristics. In the present study, a porcine RVA strain, named AHFY2022, was successfully isolated from the small intestine tissue of piglets with severe diarrhea. The AHFY2022 strain was identified
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Identification, characterization and complete genome analysis of a Vibrio anguillarum isolated from Sebastes schlegelii Microb. Pathog. (IF 3.8) Pub Date : 2024-03-11 Zeyu Li, Yungui Sun, Ruiming Tan, Yingli Gao
is an important fish pathogen in mariculture, which can infect fish with great economic losses. In this study, a isolated from was named VA1 and was identified and characterized from aspects of morphology, physiological and biochemical characteristics, 16SRNA, virulence genes, drug sensitivity, and extracellular enzyme activity. At the same time, The VA1 was investigated at the genomic level. The results
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Molecular insights into the variability and pathogenicity of Fusarium odoratissimum, the causal agent of Panama wilt disease in banana Microb. Pathog. (IF 3.8) Pub Date : 2024-03-06 Padma Tamang, Pradeep Kumar, Arpit Chauhan, Satyam Rastogi, Suchi Srivastava, Satya Narayan Jena
Fusarium wilt or Panama disease of banana caused by the hemibiotroph fungus, , also known as f.sp. Tropical Race 4 is a serious threat to banana production worldwide. Being the world's largest grower and the origins of bananas in its northeast region, India is particularly vulnerable to this deadly fungus. In the present study, a total of 163 isolates from infected banana were characterized for their
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Attenuation of Las/Rhl quorum sensing regulated virulence and biofilm formation in Pseudomonas aeruginosa PAO1 by Artocarpesin Microb. Pathog. (IF 3.8) Pub Date : 2024-03-05 Mahima S. Mohan, Simi Asma Salim, Sampathkumar Ranganathan, Paramanantham Parasuraman, V.T. Anju, Dinakara Rao Ampasala, Madhu Dyavaiah, Jung-Kul Lee, Siddhardha Busi
The emergence of multidrug resistance and increased pathogenicity in microorganisms is conferred by the presence of highly synchronized cell density dependent signalling pathway known as quorum sensing (QS). The QS hierarchy is accountable for the secretion of virulence phenotypes, biofilm formation and drug resistance. Hence, targeting the QS phenomenon could be a promising strategy to counteract
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Extracellular vesicles from Candida albicans modulate immune cells function and play a protective role in fungal keratitis Microb. Pathog. (IF 3.8) Pub Date : 2024-03-02 Huijin Duan, Fanyue Meng, Xing Liu, Pingli Qi, Xudong Peng, Cui Li, Qian Wang, Guiqiu Zhao, Jing Lin
Fungal keratitis (FK) is a highly blinding infectious corneal disease caused by pathogenic fungi. () is one of the main pathogens of fungal keratitis. Extracellular vesicles (EVs), lipid bilayer compartments released by almost all living cells, including fungi, have garnered attention for their role in pathogenic microbial infection and host immune responses in recent years. Studies have reported that
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Mechanistic insights on the antibacterial action of the kyotorphin peptide derivatives revealed by in vitro studies and Galleria mellonella proteomic analysis Microb. Pathog. (IF 3.8) Pub Date : 2024-03-02 Vitor M. de Andrade, Vitor D.M. de Oliveira, Uilla Barcick, Vasanthakumar G. Ramu, Montserrat Heras, Eduard R. Bardají, Miguel A.R.B. Castanho, André Zelanis, Aline Capella, Juliana C. Junqueira, Katia Conceição
The selected kyotorphin derivatives were tested to improve their antimicrobial and antibiofilm activity. The antimicrobial screening of the KTP derivatives were ascertained in the representative strains of bacteria, including , , and . Kyotorphin derivatives, KTP-NH, KTP–NH–DL, IbKTP, IbKTP-NH, MetKTP-DL, MetKTP-LD, were designed and synthesized to improve lipophilicity and resistance to enzymatic
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Dual-RPA assay for rapid detection and differentiation of E.granulosus and E.multilocularis Microb. Pathog. (IF 3.8) Pub Date : 2024-02-29 Shu Cai, Changjiang Wang, Fengrong Tian, Mengxiao Liu, Hongbin Yan, Zaiyan Xu, Guanggang Qu
(Eg) and (Em) are the two most widely prevalent types of echinococcosis. Several diagnostic methods have been developed for detecting Eg and Em. However, some limitations, such as being time-consuming, needing expensive instruments, or exhibiting low sensitivity, make these methods unsuitable for on-site detection. In this study, a dual-RPA assay was established to detect and differentiate Eg and Em
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Platycodon grandiflorus polysaccharide inhibits the inflammatory response of 3D4/21 cells infected with PCV2 Microb. Pathog. (IF 3.8) Pub Date : 2024-02-28 Xiaocheng Guo, Ximan Zhao, Linjue Li, Menglin Jiang, Aiqin Zhou, Yifan Gao, Pimiao Zheng, Jianzhu Liu, Xiaona Zhao
Porcine circovirus type 2 (PCV2) infection cause multi-systemic inflammation in pigs. polysaccharide (PGPS) has been reported to have the effects of immune regulation and disease resistance. Nevertheless, the role and mechanism of PGPS in the inflammatory response of 3D4/21 cells induced by PCV2 infection remain unclear. The present study aims to investigate effects of PGPS on inflammatory response
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Antibacterial activity and mechanism of cell-free supernatants of Lacticaseibacillus paracasei against Propionibacterium acnes Microb. Pathog. (IF 3.8) Pub Date : 2024-02-28 Jiahui Xu, Xiaoyong Chen, Jiajia Song, Chen Wang, Weiping Xu, Han Tan, Huayi Suo
() is an anaerobic and gram-positive bacterium involved in the pathogenesis and inflammation of acne vulgaris. This study particularly focuses on the antimicrobial effect of LPH01 against . , a bacterium that causes acne vulgaris. Fifty-seven strains were tested for their ability to inhibit growth employing the Oxford Cup and double dilution methods. The cell-free supernatant (CFS) of LPH01 demonstrated
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Impacts of intake of trichothecenes (Fusarium sporotrichioides) for dairy calves: Effects on animal growth, oxidative and inflammatory response Microb. Pathog. (IF 3.8) Pub Date : 2024-02-28 Marcela Machado, Luisa Nora, Tayse Burger Neto Zanin, Bianca F. Bissacotti, Vera M. Morsch, Marcelo Vedovatto, Giovana Pelisser, Ricardo E. Mendes, Alessandro C. Galvão, Caroline Sefrin Speroni, Eduardo M. Gloria, Murilo F. Rodrigues, Roger Wagner, Lenita M. Stefani, Aleksandro S. da Silva
The objective of the present study was to evaluate the impacts of trichothecenes () for dairy calves on animal growth, oxidative and inflammatory responses in the presence or absence of essential oils. Twelve calves weaned at 70 days of age were divided into 2 groups: T-C (control) and T-EO (essential oils - oregano, thyme, basil and rosemary) in the period of 40 days consuming ration contaminated
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Pepper defense against Ralstonia solanacearum and High-temperature stress is positively regulated by CaMYB59 Microb. Pathog. (IF 3.8) Pub Date : 2024-02-28 Ali Noman, Khairiah Mubarak Alwutayd, Muhammad Aqeel, Ansar Hussain, Muhammad Qasim, Rahmah N. Al-Qthanin, Mohammed O. Alshaharni, Fahad Mohammed Alzuaibr, Maryam M. Alomran
We have functionally evaluated a transcription factor for its role in pepper immune responses to attack and high temperature-high humidity (HTHH). Exposure to . inoculation (RSI) and HTHH resulted in up-regulation of this nucleus-localized TF. Function of this TF was confirmed by performing loss of function assay of CaMYB59 by VIGS (virus-induced gene silencing). Plants with silenced CaMYB59 displayed
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Enhancement of Streptomyces thinghirensis WAE1 for production of bioactive metabolites under different optimization strategies Microb. Pathog. (IF 3.8) Pub Date : 2024-02-28 Mohamed E. Osman, Amany A. Abo Elnasr, Eslam T. Mohamed, Ahmed H.I. Faraag
Isolation of novel bioactive metabolites from strains is a promising source for drug discovery. However, conventional screening approaches have limitations in identifying new leads due to redundant discoveries. Optimization of culture conditions is important but traditionally optimized one factor at a time, failing to consider interactions. This study addressed these gaps by enhancing metabolite production
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Piperine, a phytochemical prevents the biofilm city of methicillin-resistant Staphylococcus aureus: A biochemical approach to understand the underlying mechanism Microb. Pathog. (IF 3.8) Pub Date : 2024-02-27 Sharmistha Das, Moumita Malik, Debabrata Ghosh Dastidar, Ritwik Roy, Payel Paul, Sarita Sarkar, Poulomi Chakraborty, Alakesh Maity, Monikankana Dasgupta, Anirban Das Gupta, Sudipta Chatterjee, Ranojit Kumar Sarker, Debasish Maiti, Prosun Tribedi
Methicillin-resistant (MRSA), a drug-resistant human pathogen causes several nosocomial as well as community-acquired infections involving biofilm machinery. Hence, it has gained a wide interest within the scientific community to impede biofilm-induced MRSA-associated health complications. The current study focuses on the utilization of a natural bioactive compound called piperine to control the biofilm
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Br - nanoconjugate enhances the antibacterial efficacy of nimboloide against Flavobacterium columnare infection in Labeo rohita: A nanoinformatics approach Microb. Pathog. (IF 3.8) Pub Date : 2024-02-27 Sanjana Mishra, Prekshi Garg, Shilpi Srivastava, Prachi Srivastava
The bacterial pathogen, causes columnaris disease in globally. Major effects of this bacterial infection include skin rashes and gill necrosis. Nimbolide, the key ingredient of the leaf extract of possesses anti-bacterial properties effective against many microorganisms. Nano-informatics plays a promising role in drug development and its delivery against infections caused by multi-drug-resistant bacteria
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Antimicrobial activity of probiotic bacteria-mediated cadmium oxide nanoparticles against fish pathogens Microb. Pathog. (IF 3.8) Pub Date : 2024-02-24 Shanmugam Rajeshkumar, Santhoshkumar Jayakodi, M. Tharani, Naiyf S. Alharbi, Muthu Thiruvengadam
The current research was designed to investigate the antibacterial activity of probiotic bacteria mediated cadmium oxide nanoparticles (CdO NPs) on common fish pathogenic bacteria like , , , and . . CdO NPs were synthesized using probiotic bacteria as follows: species with different precursor of cadmium sulfate concentrations (5, 10, and 20 mM). The average crystalline sizes of the CdO NPs were determined
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Δfur mutant as a potential live attenuated vaccine (LAV) candidate protects American eels (Anguilla rostrata) from Vibrio harveyi infection Microb. Pathog. (IF 3.8) Pub Date : 2024-02-23 Qijuan Wan, Shaowei Zhai, Minxia Chen, Ming Xu, Songlin Guo
The eel farming industry is highly susceptible to . Although various types of vaccines against have been investigated, there is limited research on decreasing the virulence of s through gene knockout and utilizing it as live attenuated vaccines (LAV). In this study, we aim to develop a LAV candidate against infection in American eels () using a ferric uptake regulator () gene mutant strain of (Δ mutant)
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Gut microbiome in diabetic retinopathy: A systematic review and meta-analysis Microb. Pathog. (IF 3.8) Pub Date : 2024-02-23 Shuang Zhao, Qi Yan, Wanjing Xu, Juanmei Zhang
Changes in the gut microbiome are linked with Type 2diabetes mellitus (T2DM) development, but alterations in patients with diabetic retinopathy (DR) are still being debated. To investigate the differences in biodiversity and relative abundance of gut microbiome between patients with DR and T2DM. A comprehensive search was performed in five electronic databases (PubMed, EMBASE, Cochrane Central Register
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Evaluation of long-term immune response in cattle to botulism using a recombinant E. coli bacterin formulated with Montanide™ ISA 50 and aluminum hydroxide adjuvants Microb. Pathog. (IF 3.8) Pub Date : 2024-02-21 Clovis Moreira Jr., Rafael R. Rodrigues, Carlos E.P. da Cunha, Rafael A. Donassolo, Marcos R.A. Ferreira, Paula F. Finger, Hanna G.S. Oliveira, Karoline P. da Cruz, Ângela N. Moreira, Felipe M. Salvarani, Fabricio R. Conceição
Botulism is a severe disease caused by potent botulinum neurotoxins (BoNTs) produced by . This disease is associated with high-lethality outbreaks in cattle, which have been linked to the ingestion of preformed BoNT serotypes C and D, emphasizing the need for effective vaccines. The potency of current commercial toxoids (formaldehyde-inactivated BoNTs) is assured through tests in guinea pigs according
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Deletion of speA and aroC genes impacts the pathogenicity of Vibrio anguillarum in spotted sea bass Microb. Pathog. (IF 3.8) Pub Date : 2024-02-21 Haisheng Xie, Yajuan Huang, Shengming Wang, Jingyuan Che, Tuyan Luo, Lekang Li, Baolong Bao
is one of the major pathogens responsible for bacterial infections in marine environments, causing significant impacts on the aquaculture industry. The misuse of antibiotics leads to bacteria developing multiple drug resistances, which is detrimental to the development of the fisheries industry. In contrast, live attenuated vaccines are gradually gaining acceptance and widespread recognition. In this
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Combined analysis of the microbiome, metabolome and transcriptome of silkie chickens in response to avian pathogenic E. coli (APEC) Microb. Pathog. (IF 3.8) Pub Date : 2024-02-20 Wenqing Li, Wanli Li, Qinghan Zhao, Pinhui Wu, Xinmeng Huang, Wei Jin, Bingxun Wang, Shengli Li, Wei Liu, Guozhi Zhang, Xiangtao Kang
Avian colibacillosis is a bacterial disease caused by avian pathogenic (APEC) that results in great losses in the poultry industry every year. Individual Silkie chickens of the same breed that are given the same feed in the same feeding conditions have different levels of resistance or susceptibility to APEC. Differences in gut microbes, gut metabolites, and gene expression in the spleen of APEC-resistant
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Comparative transcriptomics reveals common and strain-specific responses of human macrophages to infection with Mycobacterium tuberculosis and Mycobacterium bovis BCG Microb. Pathog. (IF 3.8) Pub Date : 2024-02-20 Pei Li, Yang Li, Cun Chuan Wang, Li Gang Xia
(MTB) and () are closely related pathogenic mycobacteria known to cause chronic pulmonary infections in both humans and animals. Despite sharing nearly identical genomes and virulence factors, these two bacteria display variations in host tropism, epidemiology, and clinical presentations. Bacillus Calmette–Guérin (BCG) is an attenuated strain of commonly utilized as a vaccine for tuberculosis (TB)
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Synthesis and characterization of marine seagrass (Cymodocea serrulata) mediated titanium dioxide nanoparticles for antibacterial, antibiofilm and antioxidant properties Microb. Pathog. (IF 3.8) Pub Date : 2024-02-20 Mohankumar Narayanan, Suganthi Srinivasan, Chackaravarthi Gnanasekaran, Govindan Ramachandran, Chenthis Kanisha Chelliah, Govindan Rajivgandhi, Muthuchamy Maruthupandy, Franck Quero, Wen-Jun Li, Gasim Hayder, Jamal M. Khaled, Arulraj Arunachalam, Natesan Manoharan
mediated titanium dioxide nanoparticles (TiO NPs) were successfully synthesized. The XRD pattern and FTIR spectra demonstrated the crystalline structure of TiO NPs and the presence of phenols, flavonoids and alkaloids in the extract. Further SEM revealed that TiO NPs has uniform structure and spherical in shape with their size ranged from 58 to 117 nm. Antibacterial activity of TiO NPs against methicillin-resistant
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Corrigendum to “Kosakonia cowanii, a new bacterial pathogen affecting foxtail millet (Setaria italica[L.]P. Beauv.) in China” [Microb Pathog 181 (2023) 106201] Microb. Pathog. (IF 3.8) Pub Date : 2024-02-20 Yanqing Han, Xi Gao, Guoli Huang, Yindong Chang, Haili Han, Jiao Zhu, Baojun Zhang
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A novel perspective on eugenol as a natural anti-quorum sensing molecule against Serratia sp. Microb. Pathog. (IF 3.8) Pub Date : 2024-02-20 Fakher Frikha, Marwa Jardak, Sami Aifa, Sami Mnif
is commonly noted to be an opportunistic pathogen and is often associated with nosocomial infections. In addition to its high antibiotic resistance, it exhibits a wide range of virulence factors that confer pathogenicity. Targeting quorum sensing (QS) presents a potential therapeutic strategy for treating bacterial infections caused by , as it regulates the expression of various virulence factors.
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Pre-clinical safety and toxicity assessment of Limosilactobacillus fermentum NCDC 400 in murine model Microb. Pathog. (IF 3.8) Pub Date : 2024-02-19 Basavaprabhu Haranahalli Nataraj, K. Jeevan, Ajay Kumar Dang, Ravinder Nagpal, Syed Azmal Ali, Pradip V. Behare
Comprehensive safety assessment of potential probiotic strains is crucial in the selection of risk-free strains for clinical translation. This study aimed to evaluate the biosafety of NCDC 400, a potential probiotic strain, using oral toxicity tests in a Swiss albino mouse model. Mice were orally gavaged with low (10 CFU/mouse/day) and high (10 CFU/mouse/day) doses of NCDC 400 for 14 (acute), 28 (subacute)
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Blood transcriptome analysis uncovered COVID-19–myocarditis crosstalk Microb. Pathog. (IF 3.8) Pub Date : 2024-02-17 Shuang Liang, Ying-Ying Zheng, Ying Pan
The condition of COVID-19-related myocarditis has emerged as a prominent contributor to COVID-19 mortality. As the epidemic persists, its incidence continues to rise. Despite ongoing efforts, the elucidation of COVID-19-related myocarditis underlying molecular mechanisms still requires further investigation. Hub genes for COVID-19-related myocarditis were screened by integrating gene expression profile
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Screening for immune biomarkers associated with infection or protection against Ehrlichia ruminantium by RNA-sequencing analysis Microb. Pathog. (IF 3.8) Pub Date : 2024-02-16 A. Pretorius, T. Nefefe, N. Thema, J. Liebenberg, H. Steyn, M. van Kleef
Heartwater is one of the most economically important tick-borne fatal diseases of livestock. The disease is caused by the bacteria transmitted by ticks. Although there is evidence that interferon-gamma controls growth and that cellular immune responses are protective, an effective recombinant vaccine for this disease is lacking. Analyses of markers associated with infection as well as protection will
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Macrophage migration inhibitory factor favors Neospora caninum infection in mice Microb. Pathog. (IF 3.8) Pub Date : 2024-02-16 Vanessa Resende Souza Silva, Caroline Martins Mota, Lydiane Parreira Maia, Flávia Batista Ferreira, Vanessa dos Santos Miranda, Neide Maria Silva, Eloísa A. Vieira Ferro, José Roberto Mineo, Tiago Wilson Patriarca Mineo
is a protozoan parasite with worldwide incidence, acting as a major cause of reproductive failures in ruminants and neuromuscular symptoms in dogs. Macrophage Migration Inhibitory Factor (MIF) is produced by several cell types and exhibits a central role in immune responses against intracellular pathogens. The present study aimed to comprehend the role of MIF in the relationship between and its host
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Evaluation of reference genes for gene expression analysis by real-time quantitative PCR (qPCR) in different tissues from mice infected by Ascaris suum Microb. Pathog. (IF 3.8) Pub Date : 2024-02-14 Jorge Lucas Nascimento Souza, Camila de Almeida Lopes, Thais Leal-Silva, Flaviane Vieira-Santos, Chiara Cássia Oliveira Amorim, Luiza de Lima Silva Padrão, Ana Rafaela Antunes Porto, Ricardo Toshio Fujiwara, Remo Castro Russo, Lilian Lacerda Bueno
Human ascariasis is the most prevalent helminth infection, affecting 445 million people worldwide. To better understand the impact of the immune system on the pathophysiology of individuals infected with , mice have been used as experimental models. The RT-qPCR technique is a critical auxiliary tool of investigation used to quantify mRNA levels. However, proper normalization using reference genes is
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Treatment of dental biofilm-forming bacterium Streptococcus mutans using tannic acid-mediated gold nanoparticles Microb. Pathog. (IF 3.8) Pub Date : 2024-02-13 Kaviya Selvaraj, Lekha Sree Venkatesan, Dhanraj Ganapathy, Palanivel Sathishkumar
Biosynthesized gold nanoparticles (AuNPs) are highly attracted as a biocompatible nanodrug to treat various diseased conditions in humans. In this study, phytochemical tannic acid-mediated AuNPs (TA-AuNPs) are successfully synthesized and tested for antibacterial and antibiofilm activity against dental biofilm-forming biofilm. The synthesized TA-AuNPs are appeared as spherical in shape with an average
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Phage therapy: A renewed approach against oral diseases caused by Enterococcus faecalis infections Microb. Pathog. (IF 3.8) Pub Date : 2024-02-13 Dan yang, Yingying Xiang, Fei Song, Haiyan Li, Xiuling Ji
Antibiotics play an important role in the treatment of infectious diseases. Long-term overuse or misuse of antibiotics, however, has triggered the global crisis of antibiotic resistance, bringing challenges to treating clinical infection. Bacteriophages (phages) are the viruses infecting bacterial cells. Due to high host specificity, high bactericidal activity, and good biosafety, phages have been
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The antimicrobial property of JY-1, a complex mixture of Traditional Chinese Medicine, is linked to it abilities to suppress biofilm formation and disrupt membrane permeability Microb. Pathog. (IF 3.8) Pub Date : 2024-02-13 Ying Ji, Ji Han, Munika Moses, Di Wang, Lei Wu, Wenwen Xue, Lu Sun, Bo Xu, Changbin Chen, Yanwei Xiang, Xinhua Huang
The substantial increase of infections, caused by novel, sudden, and drug-resistant pathogens, poses a significant threat to human health. While numerous studies have demonstrated the antibacterial and antiviral effects of Traditional Chinese Medicine, the potential of a complex mixture of traditional Chinese Medicine with a broad-spectrum antimicrobial property remains underexplored. This study aimed
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Structural vaccinology, molecular simulation and immune simulation approaches to design multi-epitopes vaccine against John Cunningham virus Microb. Pathog. (IF 3.8) Pub Date : 2024-02-12 Muhammad Suleman, Tariq Aziz Khan, Hadiqa Ejaz, Sabahat Maroof, Abdulrahman Alshammari, Norah A. Albekairi, Haji Khan, Yasir Waheed, Abbas Khan, Dong-Qing Wei, Sergio Crovella
The JCV (John Cunningham Virus) is known to cause progressive multifocal leukoencephalopathy, a condition that results in the formation of tumors. Symptoms of this condition such as sensory defects, cognitive dysfunction, muscle weakness, homonosapobia, difficulties with coordination, and aphasia. To date, there is no specific and effective treatment to completely cure or prevent John Cunningham polyomavirus
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Multifunctional integration of tungsten oxide (WO3) coating: A versatile approach for enhanced performance of antibiotics against single mixed bacterial infections Microb. Pathog. (IF 3.8) Pub Date : 2024-02-08 Arslan Bashir, Shanza Rauf Khan, Amjad Islam Aqib, Laiba Shafique, Farid S. Ataya
Nanomaterials containing tungsten (TNMs), characterized by diverse nanostructures had been extensively used in biomedical sector. Despite numerous reports focusing on TNM applications in specific biomedical areas, there is a noticeable absence of comprehensive studies that focused on detailed characterization of nanomaterials along with their biological applications. The present work described the
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A high concentrate diet inhibits forkhead box protein A2 expression, and induces oxidative stress, mitochondrial dysfunction and mitochondrial unfolded protein response in the liver of dairy cows Microb. Pathog. (IF 3.8) Pub Date : 2024-02-08 Ran Kun Zuo, Can Wang, Zhi Yuan Yu, Hui Min Shi, Xiao Kun Song, Shen Dong Zhou, Na Na Ma, Guang Jun Chang, Xiang Zhen Shen
High-concentrate diet induce subacute ruminal acidosis (SARA) and cause liver damage in ruminants. It has been reported that forkhead box protein A2 (FOXA2) can enhance mitochondrial membrane potential but its function in mitochondrial dysfunction induced by high concentrate diets is still unknown. Therefore, the aim of this study was to elucidate the effect of high-concentrate (HC) diet on hepatic
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Associations between rheumatoid arthritis and intestinal flora, with special emphasis on RA pathologic mechanisms to treatment strategies Microb. Pathog. (IF 3.8) Pub Date : 2024-02-06 Yanhui Peng, Yurong Huang, Hui Li, Chen Li, Yajie Wu, Xiaomei Wang, Qiang Wang, Juan He, Chenggui Miao
Rheumatoid arthritis (RA) is an autoimmune inflammatory disease that primarily affects the joints. Individuals at risk for RA and people with RA develop intestinal dysbiosis. The changes in intestinal flora composition in preclinical and confirmed RA patients suggest that intestinal flora imbalance may play an important role in the induction and persistence of RA. Based on the current research on the
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Microbiome analysis reveals the differences in gut fungal community between Dutch Warmblood and Mongolian horses Microb. Pathog. (IF 3.8) Pub Date : 2024-02-02 Yanfang Lan, Yunyun Hu, Yingfang Guo, Farah Ali, Nouman Amjad, Quanli Ouyang, Mikhlid H. Almutairi, Dongjing Wang
Similar to gut bacterial community, gut fungal community are also an important part of the gut microbiota and play crucial roles in host immune regulation and metabolism. However, most studies have focused on the gut bacterial community, and research on the gut fungal community has been limited. Dutch Warmblood (DWH) and Mongolian horses (MGH) are important equine breeds, but little research has been
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Study on the relationship between tea polyphenols alleviating osteoporosis and the changes of microorganism-metabolite-intestinal barrier Microb. Pathog. (IF 3.8) Pub Date : 2024-02-02 Xin Wen, Panyang Wu, Feng Li, Guofu Pi
Tea polyphenols are known to alleviate osteoporosis; however, the role of intestinal flora in this process has not been studied. This research employed 16s rRNA sequencing and non-targeted metabonomics to investigate the potential link between osteoporosis mitigation and changes in intestinal flora. MicroCT and tissue staining results demonstrated that tea polyphenols improved bone microstructure,
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Loss of expression of the glutamate dehydrogenase (gdh) of Streptococcus suis serotype 2 compromises growth and pathogenicity Microb. Pathog. (IF 3.8) Pub Date : 2024-02-02 Lauren Chittick, Ogi Okwumabua
serotype 2 is a zoonotic agent that causes substantial economic losses to the swine industry and threatens human public health. Factors that contribute to its ability to cause disease are not yet fully understood. Glutamate dehydrogenase (GDH) is an enzyme found in living cells and plays vital roles in cellular metabolism. It has also been shown to affect pathogenic potential of certain bacteria. In
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Pseudomonas aeruginosa outer membrane vesicle-packed sRNAs can enter host cells and regulate innate immune responses Microb. Pathog. (IF 3.8) Pub Date : 2024-02-01 Zhen Xie, Xiao Wang, Yangyang Huang, Shukun Chen, Mohua Liu, Fuhua Zhang, Mengyuan Li, Xiao Wang, Yanchao Gu, Yadong Yang, Xihui Shen, Yao Wang, Yang Xu, Lei Xu
Bacterial outer membrane vesicles (OMVs) can package and deliver virulence factors into host cells, which is an important mechanism mediating host-pathogen interactions. It has been reported that small RNAs (sRNAs) can be packed into OMVs with varying relative abundance, which might affect the function and/or stability of host mRNAs. In this study, we used OptiPrep density gradient ultra-high-speed
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Description of nasopharyngeal bacterial pathogens associated with different SARS-CoV-2 variants Microb. Pathog. (IF 3.8) Pub Date : 2024-02-01 Ornella La Fortune Tchoupou Saha, Grégory Dubourg, Abdourahamane Yacouba, Raphael Tola, Didier Raoult, Jean-Christophe Lagier
The emergence of the coronavirus pandemic facilitated the acquisition of mutations in the SARS-CoV-2 genome, resulting in the appearance of new variants over the past three years. We previously identified several taxa associated with the clinical outcome of COVID-19 disease in a retrospective study involving 120 patients (infected patients and negative subjects). However, little is known about whether
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Comparative evaluation of anti-biofilm and anti- adherence potential of plant extracts against Streptococcus mutans: A therapeutic approach for oral health Microb. Pathog. (IF 3.8) Pub Date : 2024-01-29 Preeti Pallavi, Pragnya Paramita Sahoo, Sudip Kumar Sen, Sangeeta Raut
Dental caries predominantly attributed to the cariogenic nature of Streptococcus mutans, continue to pose a substantial global challenge to oral health. In response to this challenge, this study aimed to evaluate the effectiveness of leaf extracts (LEs) and essential oils (EOs) derived from different medicinal plants in inhibiting the growth of Streptococcus mutans biofilm. In vitro and in silico approaches
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Microbiological study of the auditory canal in dairy calves with otitis media Microb. Pathog. (IF 3.8) Pub Date : 2024-01-29 Samad Lotfollahzadeh, Taghi Zahraei Salehi, Mohammad Zare Esmatabadi, Mohammad Ramezankhani, Iraj Ashrafi Tamai, Arman Shokri, Mostafa Abdollahi
Otitis media (OM) in calves, is caused by different bacteria. OM treatment requires identification of etiological agents and antibiotic sensitivity testing. The gold standard method of bacteriological study of OM is tympanocentesis, but using this technique in farm condition would be difficult. As a hypothesis, it is possible that bacteriologic examining the auditory canal can help to accelerate the
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The modulatory effect of Lactobacillus gasseri ATCC 33323 on autophagy induced by extracellular vesicles of Helicobacter pylori in gastric epithelial cells in vitro Microb. Pathog. (IF 3.8) Pub Date : 2024-01-23 Zahra Sadeghloo, Parvaneh Saffarian, Mojdeh Hakemi-Vala, Amir Sadeghi, Abbas Yadegar
Helicobacter pylori has been recognized as a true pathogen, which is associated with various gastroduodenal diseases, and gastric adenocarcinoma. The crosstalk between H. pylori virulence factors and host autophagy remains challenging. H. pylori can produce extracellular vesicles (EVs) that contribute to gastric inflammation and malignancy. Some probiotic strains have been documented to modulate cell
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Host protein EPCAM interacting with EtMIC8-EGF is essential for attachment and invasion of Eimeria tenella in chickens Microb. Pathog. (IF 3.8) Pub Date : 2024-01-26 Lingyu Sun, Chao Li, Ningning Zhao, Bingxiang Wang, Hongmei Li, Hairong Wang, Xiao Zhang, Xiaomin Zhao
The five epidermal growth factor-like domains (EGF) of Eimeria tenella microneme protein 8 (EtMIC8) (EtMIC8-EGF) plays a vital role in host cell attachment and invasion. These processes require interactions between parasite proteins and receptors on the surface of host cells. In this study, five chicken membrane proteins potentially interacting with EtMIC8-EGF were identified using the GST pull-down
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The cyclic AMP (cAMP) phosphodiesterase CpdA required for growth, biofilm formation, motility and pathogenicity of Edwardsiella piscicida Microb. Pathog. (IF 3.8) Pub Date : 2024-01-19 Yidong Cai, Jinggang Dong, Jianqiang Huang, Jiaojiao He, Yonghua Hu, Zhihai Sui, Ping Tang
Edwardsiella piscicida is a severe fish pathogen with wide host range, causing the huge economic losses in the aquaculture industry. Cyclic adenosine monophosphate (cAMP) as an important second messenger regulates the physiological and behavioral responses to environmental cues in eukaryotic and prokaryotic. The intracellular level of cAMP for effective activity is tightly controlled by the synthesis
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Antimicrobial, antibiofilm, and antivirulence properties of Eisenia bicyclis-extracts and Eisenia bicyclis-gold nanoparticles towards microbial pathogens Microb. Pathog. (IF 3.8) Pub Date : 2024-01-24 DoKyung Oh, Fazlurrahman Khan, Seul-Ki Park, Du-Min Jo, Nam-Gyun Kim, Won-Kyo Jung, Young-Mog Kim
Nanomaterials derived from seaweed have developed as an alternative option for fighting infections caused by biofilm-forming microbial pathogens. This research aimed to discover potential seaweed-derived nanomaterials with antimicrobial and antibiofilm action against bacterial and fungal pathogens. Among seven algal species, the extract from Eisenia bicyclis inhibited biofilms of Klebsiella pneumoniae
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Soluble Endoglin and Syndecan-1 levels predicts the clinical outcome in COVID-19 patients Microb. Pathog. (IF 3.8) Pub Date : 2024-01-23 Remya Rajan, Mohamed Hanifah, Vignesh Mariappan, Monica Anand, Agieshkumar Balakrishna Pillai
Endothelial instability is reported to be involved in the pathogenesis of COVID-19. The mechanism that regulates the endothelial dysfunction and disease virulence is not known. Studies on proteins that are released into circulation by activated endothelial cells may provide some means to understand the disease manifestation. The study investigated the circulating levels of two molecules Endoglin (Eng)
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The role of the Notch signaling pathway in bacterial infectious diseases Microb. Pathog. (IF 3.8) Pub Date : 2024-01-23 Zhengwei Yin, Yuejie Zhu, Juan Shi, Yueyue he, Fengbo Zhang
The Notch signaling pathway is the most crucial link in the normal operation and maintenance of physiological functions of mammalian life processes. Notch receptors interact with ligands and this leads to three cleavages and goes on to enter the nucleus to initiate the transcription of target genes. The Notch signaling pathway deeply participates in the differentiation and function of various cells
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The proteolytic activity in inflammatory bowel disease: insight from gut microbiota Microb. Pathog. (IF 3.8) Pub Date : 2024-01-23 Jun-Jie Hou, Liang Ding, Tao Yang, Yan-Fei Yang, Yue-Ping Jin, Xiao-Ping Zhang, A-Huo Ma, Yue-Hua Qin
Inflammatory bowel disease (IBD) is a chronic, recurrent inflammatory disease caused by the destruction of the intestinal mucosal epithelium that affects a growing number of people worldwide. Although the etiology of IBD is complex and still elucidated, the role of dysbiosis and dysregulated proteolysis is well recognized. Various studies observed altered composition and diversity of gut microbiota