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Subgingival microbiota in a population with and without cognitive dysfunction J. Oral Microbiol. (IF 3.939) Pub Date : 2021-01-15 Jacob Holmer; Velma Aho; Maria Eriksdotter; Lars Paulin; Milla Pietiäinen; Petri Auvinen; Marianne Schultzberg; Pirkko J. Pussinen; Kåre Buhlin
ABSTRACT Aim: The aim of this study was to compare the subgingival microbiota of people with Alzheimer´s disease (AD), mild cognitive impairment (MCI), subjective cognitive decline (SCD) and cognitively healthy individuals. Materials and methods: The study population was recruited from 2013 to 2017 and comprised 132 cases recently diagnosed with AD (n = 46), MCI (n = 40) or SCD (n = 46), and 63 cognitively
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Enterococcus faecalis shifts macrophage polarization toward M1-like phenotype with an altered cytokine profile J. Oral Microbiol. (IF 3.939) Pub Date : 2021-01-04 Mohamed Mohamed Elashiry; Fucong Tian; Mahmoud Elashiry; Rana Zeitoun; Ranya Elsayed; Matthew L. Andrews; Brian E. Bergeon; Christopher Cutler; Franklin Tay
ABSTRACT Background: The macrophage is an innate immune defense cell involved in pathogen recognition and clearance. Aim: In view of the diversity of the macrophage phenotype and function, the present study investigated how Enterococcus faecalis infection affects the differentiation, phenotype and cytokine profile of macrophages. Methods: Murine bone marrow-derived stem cells were co-cultured with
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Oral microbiome in down syndrome and its implications on oral health J. Oral Microbiol. (IF 3.939) Pub Date : 2020-12-30 Jesse R. Willis; Susana Iraola-Guzmán; Ester Saus; Ewa Ksiezopolska; Luca Cozzuto; Luis A. Bejarano; Nuria Andreu-Somavilla; Miriam Alloza-Trabado; Anna Puig-Sola; Andrea Blanco; Elisabetta Broglio; Carlo Carolis; Jochen Hecht; Julia Ponomarenko; Toni Gabaldón
ABSTRACT Introduction: The oral cavity harbors an abundant and diverse microbial community (i.e. the microbiome), whose composition and roles in health and disease have been the focus of intense research. Down syndrome (DS) is associated with particular characteristics in the oral cavity, and with a lower incidence of caries and higher incidence of periodontitis and gingivitis compared to control populations
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Recombinant bacteriophage T4 Rnl1 impacts Streptococcus mutans biofilm formation J. Oral Microbiol. (IF 3.939) Pub Date : 2020-12-24 Juxiu Chen; Zhanyi Chen; Keyong Yuan; Zhengwei Huang; Mengying Mao
ABSTRACT Bacteriophage T4 RNA ligase 1 (T4 Rnl1) can be stably expressed in many bacteria and has been reported to affect the bioactivity of the host bacteria. Recently, we constructed bacteriophage T4 Rnl1 expressing system in Streptococcus mutans, a crucial biofilm-forming and dental caries-causing oral pathogen. Here, we characterized the function of recombinant bacteriophage T4 Rnl1 in biofilm
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Neutrophils exhibit an individual response to different oral bacterial biofilms J. Oral Microbiol. (IF 3.939) Pub Date : 2020-12-09 Carina Mikolai; Katja Branitzki-Heinemann; Alexandra Ingendoh-Tsakmakidis; Meike Stiesch; Maren von Köckritz-Blickwede; Andreas Winkel
ABSTRACT Oral innate immunity is led by neutrophils. It is still unclear how their main antimicrobial mechanisms against different biofilms may contribute to balance or dysregulation in the oral cavity. We investigated the capacity of commensal (Streptococcus oralis) and pathogenic (Porphyromonas gingivalis or Aggregatibacter actinomycetemcomitans) monospecies biofilms to induce or to inhibit selected
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Salivary bacterial shifts in oral leukoplakia resemble the dysbiotic oral cancer bacteriome J. Oral Microbiol. (IF 3.939) Pub Date : 2020-12-09 Divya Gopinath; Rohit Kunnath Menon; Chong Chun Wie; Moinak Banerjee; Swagatika Panda; Deviprasad Mandal; Paresh Kumar Behera; Susanta Roychoudhury; Supriya Kheur; Michael George Botelho; Newell W. Johnson
ABSTRACT Objective: While some oral carcinomas appear to arise de novo, others develop within long-standing conditions of the oral cavity that have malignant potential, now known as oral potentially malignant disorders (OPMDs). The oral bacteriome associated with OPMD has been studied to a lesser extent than that associated with oral cancer. To characterize the association in detail we compared the
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Porphyromonas gingivalis laboratory strains and clinical isolates exhibit different distribution of cell surface and secreted gingipains J. Oral Microbiol. (IF 3.939) Pub Date : 2020-12-09 Christine A. Seers; A. Sayeed M. Mahmud; N. Laila Huq; Keith J. Cross; Eric C. Reynolds
ABSTRACT Background: The cell-surface cysteine proteinases RgpA, RgpB (Arg-gingipain), and Kgp (Lys-gingipain) are major virulence factors of P. gingivalis, a keystone pathogen in the development of destructive periodontal disease. The gingipains function as proteinases and transpeptidases utilising small peptides such as glycylglycine as acceptor molecules. However, the characteristics of the gingipains
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Performance of at-home self-collected saliva and nasal-oropharyngeal swabs in the surveillance of COVID-19 J. Oral Microbiol. (IF 3.939) Pub Date : 2020-12-09 Paulo H. Braz-Silva; Ana C. Mamana; Camila M. Romano; Alvina C. Felix; Anderson V. de Paula; Noeli E Fereira; Lewis F. Buss; Tania R. Tozetto-Mendoza; Rafael A. V. Caixeta; Fabio E. Leal; Regina M. Z. Grespan; João C. S. Bizário; Andrea B. C. Ferraz; Dipak Sapkota; Simone Giannecchini; Kelvin K. To; Alain Doglio; Maria C. Mendes-Correa
ABSTRACT Background: SARS-CoV-2 quickly spreads in the worldwide population, imposing social restrictions to control the infection, being the massive testing another essential strategy to break the chain of transmission. Aim: To compare the performance of at-home self-collected samples – saliva and combined nasal-oropharyngeal swabs (NOP) – for SARS-CoV-2 detection in a telemedicine platform for COVID-19
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Fusobacteria modulate oral carcinogenesis and promote cancer progression J. Oral Microbiol. (IF 3.939) Pub Date : 2020-11-30 Amani M. Harrandah; Sasanka S. Chukkapalli; Indraneel Bhattacharyya; Ann Progulske-Fox; Edward K. L. Chan
ABSTRACT Background: Evidence suggest periodontal bacterial infection can contribute to oral cancer initiation and progression. Aim: To investigate the effects of periodontal bacteria on oral cancer cell behavior using a cell-based system and a mouse carcinogenesis model. Methods: Oral cancer cell lines were polyinfected with four periodontal bacteria. Cytokine levels and relative changes in oncogene
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Potential implications of SARS-CoV-2 oral infection in the host microbiota J. Oral Microbiol. (IF 3.939) Pub Date : 2020-11-29 Zhenting Xiang; Hyun Koo; Qianming Chen; Xuedong Zhou; Yuan Liu; Aurea Simon-Soro
ABSTRACT The oral cavity, as the entry point to the body, may play a critical role in the pathogenesis of SARS-CoV-2 infection that has caused a global outbreak of the coronavirus disease 2019 (COVID-19). Available data indicate that the oral cavity may be an active site of infection and an important reservoir of SARS-CoV-2. Considering that the oral surfaces are colonized by a diverse microbial community
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Periodontal tissues are targets for Sars-Cov-2: a post-mortem study J. Oral Microbiol. (IF 3.939) Pub Date : 2020-11-26 Bruno Fernandes Matuck; Marisa Dolhnikoff; Gilvan V. A. Maia; Daniel Isaac Sendyk; Amanda Zarpellon; Sara Costa Gomes; Amaro Nunes Duarte-Neto; João Renato Rebello Pinho; Michele Soares Gomes-Gouvêa; Suzana C.O. M. Sousa; Thais Mauad; Paulo Hilário do Nascimento Saldiva; Paulo H. Braz-Silva; Luiz Fernando Ferraz da Silva
ABSTRACT Background: The ability of coronavirus SARS-CoV-2 to spread is one of the determinants of the COVID-19 pandemic status. Until June 2020, global COVID-19 cases surpassed 10 million. Asymptomatic patients, with no respiratory impairment, are believed to be responsible for more than 80% of the transmission. Other viruses have been consistently detected in periodontal tissues. Objective: The aim
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Tetracycline and multidrug resistance in the oral microbiota: differences between healthy subjects and patients with periodontitis in Spain J. Oral Microbiol. (IF 3.939) Pub Date : 2020-11-23 Alexandre Arredondo; Vanessa Blanc; Carolina Mor; José Nart; Rubén León
ABSTRACT Introduction: Antibiotic resistance is widely found even among bacterial populations not having been exposed to selective pressure by antibiotics, such as tetracycline. In this study we analyzed the tetracycline-resistant subgingival microbiota of healthy subjects and of patients with periodontitis, comparing the prevalence of tet genes and their multidrug resistance profiles. Methods: Samples
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Porphyromonas, Treponema, and Mogibacterium promote IL8/IFNγ/TNFα-based pro-inflammation in patients with medication-related osteonecrosis of the jaw J. Oral Microbiol. (IF 3.939) Pub Date : 2020-11-23 Qingxiang Li; Yinfei Pu; Han Lu; Ning Zhao; Yifei Wang; Yuxing Guo; Chuanbin Guo
ABSTRACT Objective: Refractory infection is an important factor affecting the progression of medication-related osteonecrosis of the jaw (MRONJ) from clinical stage I to stage II/III. The aim of this study was to explore the distribution of bacteria and their association with the inflammatory pathway of stage II/III MRONJ. Materials and Methods: Nine specimens of fresh inflammation tissue, located
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Susceptibility to biofilm formation on 3D-printed titanium fixation plates used in the mandible: a preliminary study J. Oral Microbiol. (IF 3.939) Pub Date : 2020-10-29 Lukasz Palka; Justyna Mazurek-Popczyk; Katarzyna Arkusz; Katarzyna Baldy-Chudzik
ABSTRACT Background: In the oral and maxillofacial surgery, fixation plates are commonly used for the stabilization of bone fragments. Additive manufacturing has enabled us to design and create personalized fixation devices that would ideally fit any given fracture. Aim: The aim of the present preliminary study was to assess the susceptibility of 3D-printed titanium fixation plates to biofilm formation
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Lactobacillus rhamnosus strains of oral and vaginal origin show strong antifungal activity in vitro J. Oral Microbiol. (IF 3.939) Pub Date : 2020-10-18 Mette Rose Jørgensen; Pernille Thestrup Rikvold; Mads Lichtenberg; Peter Østrup Jensen; Camilla Kragelund; Svante Twetman
ABSTRACT Background: Intake of probiotic bacteria may prevent oral Candida infection. Objective: To screen the antifungal activity of 14 Lactobacillus candidate strains of human origin, against six opportunistic C. albicans and non-albicans species. A second aim was to study the acid production of the four strains showing the strongest antifungal activity. Methods: We used an agar overlay growth inhibition
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Response of Salivary Microbiota to Caries Preventive Treatment in Aboriginal and Torres Strait Islander Children J. Oral Microbiol. (IF 3.939) Pub Date : 2020-10-11 Emily Skelly; Newell W. Johnson; Kostas Kapellas; Jeroen Kroon; Ratilal Lalloo; Laura Weyrich
ABSTRACT A once-annual caries preventive (Intervention) treatment was offered to Aboriginal and Torres Strait Islander schoolchildren—a population with disproportionately poorer oral health than non-Indigenous Australian children—in the Northern Peninsula Area (NPA) of Far North Queensland (FNQ), which significantly improved their oral health. Here, we examine the salivary microbiota of these children
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Production and characteristics of a novel chicken egg yolk antibody (IgY) against periodontitis-associated pathogens J. Oral Microbiol. (IF 3.939) Pub Date : 2020-10-12 Xiangguang Li; Pan He; Linjin Yu; Qiyi He; Chenggang Jia; Huiyi Yang; Minglei Lu; Xiuting Wei; Suqing Zhao
ABSTRACT Periodontitis is a bacterial biofilm-induced oral disease, mostly caused by Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) and Porphyromonas gingivalis (P. gingivalis). Oral administration of chicken egg yolk antibody (IgY) is a promising nutritional strategy to control pathogen infections. The objective of this study was to produce an A. actinomycetemcomitans- and P. gingivalis-specific
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Oral dysbiosis induced by Porphyromonas gingivalis is strain-dependent in mice J. Oral Microbiol. (IF 3.939) Pub Date : 2020-10-12 Emile Boyer; Patricia Leroyer; Ludivine Malherbe; Shao Bing Fong; Olivier Loréal; Martine Bonnaure Mallet; Vincent Meuric
ABSTRACT Background: Porphyromonas gingivalis strain W83, one of the most widely investigated, is considered virulent in the context of periodontitis. The recently isolated P. gingivalis TDC60 has been reported to be highly pathogenic, although it has not yet been investigated in a mouse periodontitis model by oral gavage. Aim: Our aim was to compare the virulence of both strains by evaluating their
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Investigation of the potential regulator proteins associated with the expression of major surface protein and dentilisin in Treponema denticola J. Oral Microbiol. (IF 3.939) Pub Date : 2020-10-11 Kazuyuki Ishihara; Yuki Arai; Yuichiro Kikuchi; Kazuko Okamoto-Shibayama; Eitoyo Kokubu; Seikou Shintani
ABSTRACT Objective Treponema denticola is involved in ‘chronic’ periodontitis pathogenesis. The mechanism underlying the regulation of the expression of its virulence factors, such as major surface protein (Msp) and prolyl-phenylalanine specific protease (dentilisin) is yet to be clarified. We determined the gene expression profiles of Msp- and dentilisin-deficient mutants of T. denticola to identify
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Activity of five antimicrobial peptides against periodontal as well as non-periodontal pathogenic strains J. Oral Microbiol. (IF 3.939) Pub Date : 2020-10-07 Katharina Enigk; Holger Jentsch; Arne C. Rodloff; Klaus Eschrich; Catalina-Suzana Stingu
ABSTRACT Background: Due to the increasing emergence of multi-resistant bacteria the search for alternative antimicrobial substances is of high interest. Promising agents are antimicrobial peptides which are host defense molecules of the innate immune system in a wide range of different species. Objectives: The aim of this study was to assess the activity of nisin, melittin, lactoferrin, parasin-1
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Interaction between genetic factors, Porphyromonas gingivalis and microglia to promote Alzheimer’s disease J. Oral Microbiol. (IF 3.939) Pub Date : 2020-09-16 Ingar Olsen; Sim K. Singhrao
In late-onset Alzheimer disease (AD) pathogenesis, genes, infections and immunity could be significant factors. We have reviewed if the keystone periodontal pathogen Porphyromonas gingivalis may affect genes and microglia (primary immune cells in the brain) to promote AD development. Genes for apolipoprotein, clusterin, CD33, triggering receptor expressed on myeloid cells-2 (TREM-2), tyrosine kinase
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Oral Candida albicans colonization in healthy individuals: prevalence, genotypic diversity, stability along time and transmissibility J. Oral Microbiol. (IF 3.939) Pub Date : 2020-09-14 Ângela Gerós-Mesquita; Joana Carvalho-Pereira; Ricardo Franco-Duarte; Armandino Alves; Hernâni Gerós; Célia Pais; Paula Sampaio
In this study, 181 healthy individuals, including 29 couples, were analysed regarding oral yeast colonization using a culture-based approach. Results showed that 39% of the individuals were yeast carriers, 89% being colonized with Candida albicans, 5% with C. guilliermondi, 3% with C. lusitaniae and 3% with C. parapsilosis. Sixty-two percent of the couples had at least one member colonized. Colonization
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Grade C molar-incisor pattern periodontitis subgingival microbial profile before and after treatment J. Oral Microbiol. (IF 3.939) Pub Date : 2020-09-13 Irina M. Velsko; Peter Harrison; Natalia Chalmers; Jennifer Barb; Hong Huang; Ikramuddin Aukhil; Luciana Shaddox
Aim: This study evaluated the influence of periodontal therapy on the microbiological profile of individuals with Grade C Molar-Incisor Pattern Periodontitis (C/MIP). Methods: Fifty-three African-American participants between the ages of 5–25, diagnosed with C/MIP were included. Patients underwent full mouth mechanical debridement with systemic antibiotics (metronidazole 250 mg + amoxicillin 500 mg
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Isolation and cultivation of candidate phyla radiation Saccharibacteria (TM7) bacteria in coculture with bacterial hosts J. Oral Microbiol. (IF 3.939) Pub Date : 2020-09-06 Pallavi P. Murugkar; Andrew J. Collins; Tsute Chen; Floyd E. Dewhirst
Background The vast majority of bacteria on earth have not yet been cultivated. There are many bacterial phyla with no cultivated examples including most members of the Candidate Phylum Radiation with the exception of human oral isolates from the phylum Saccharibacteria. Aims The aims of this research were to develop reproducible methods and validate approaches for the cultivation of human oral Saccharibacteria
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Correction J. Oral Microbiol. (IF 3.939) Pub Date : 2020-09-02
(2020). Correction. Journal of Oral Microbiology. Ahead of Print.
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Metabolic cooperativity between Porphyromonas gingivalis and Treponema denticola. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-08-24 Lin Xin Kin,Catherine A Butler,Nada Slakeski,Brigitte Hoffmann,Stuart G Dashper,Eric C Reynolds
Background Porphyromonas gingivalis and Treponema denticola are proteolytic periodontopathogens that co-localize in polymicrobial subgingival plaque biofilms, display in vitro growth symbiosis and synergistic virulence in animal models of disease. These symbioses are underpinned by a range of metabolic interactions including cooperative hydrolysis of glycine-containing peptides to produce free glycine
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Modulation of pathogenic oral biofilms towards health with nisin probiotic. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-08-24 Allan Radaic,Changchang Ye,Brett Parks,Li Gao,Ryutaro Kuraji,Erin Malone,Pachiyappan Kamarajan,Ling Zhan,Yvonne L Kapila
Background Oral dysbiosis is an imbalance in the oral microbiome and is associated with a variety of oral and systemic diseases, including periodontal disease, caries, and head and neck/oral cancer. Although antibiotics can be used to control this dysbiosis, they can lead to adverse side effects and superinfections. Thus, novel strategies have been proposed to address these shortcomings. One strategy
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Probiotics alter biofilm formation and the transcription of Porphyromonas gingivalis virulence-associated genes. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-08-20 Karin Hitomi Ishikawa,Daniela Mita,Dione Kawamoto,Jacques Robert Nicoli,Emmanuel Albuquerque-Souza,Maria Regina Lorenzetti Simionato,Marcia Pinto Alves Mayer
ABSTRACT Background and Objective The potential of probiotics on the prevention and control of periodontitis and other chronic inflammatory conditions has been suggested. Lactobacillus and Bifidobacterium species influence P. gingivalis interaction with gingival epithelial cells (GECs) but may not act in a unique way. In order to select the most appropriate probiotic against P. gingivalis, we aimed
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Inhibitory effects of 2-methacryloyloxyethyl phosphorylcholine polymer on the adherence of bacteria causing upper respiratory tract infection J. Oral Microbiol. (IF 3.939) Pub Date : 2020-08-20 Hiroyuki Iuchi; Junichiro Ohori; Takayuki Kyutoku; Kotoko Ito; Masaki Kawabata
ABSTRACT Objective We aimed to investigate the inhibitory effect of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer on the adherence of Streptococcus pneumoniae (Spn) and nontypeable Haemophilus influenzae (NTHi) in vitro and in vivo. Materials and Methods Phosphorylcholine (PC) expression of 21 strains each of Spn and NTHi was evaluated using fluorescence-activated cell sorting; the adherence
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Oral mycobiome identification in atopic dermatitis, leukemia, and HIV patients – a systematic review J. Oral Microbiol. (IF 3.939) Pub Date : 2020-08-17 Camila Stofella Sodré; Paulo Matheus Guerra Rodrigues; Mayra Stambovsky Vieira; Alexandre Marques Paes da Silva; Lucio Souza Gonçalves; Marcia Gonçalves Ribeiro; Dennis de Carvalho Ferreira
ABSTRACT Introduction Oral mycobiome profiling is important to understand host–pathogen interactions that occur in various diseases. Invasive fungal infections are particularly relevant for patients who have received chemotherapy and for those who have HIV infection. In addition, changes in fungal microbiota are associated with the worsening of chronic conditions like atopic dermatitis (AD). This work
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Oral mycobiome identification in atopic dermatitis, leukemia, and HIV patients - a systematic review. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-08-17 Camila Stofella Sodré,Paulo Matheus Guerra Rodrigues,Mayra Stambovsky Vieira,Alexandre Marques Paes da Silva,Lucio Souza Gonçalves,Marcia Gonçalves Ribeiro,Dennis de Carvalho Ferreira
Introduction Oral mycobiome profiling is important to understand host-pathogen interactions that occur in various diseases. Invasive fungal infections are particularly relevant for patients who have received chemotherapy and for those who have HIV infection. In addition, changes in fungal microbiota are associated with the worsening of chronic conditions like atopic dermatitis (AD). This work aims
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Identification and functional analysis of glutamine transporter in Streptococcus mutans. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-08-04 Yuko Morikawa,Setsuyo Morimoto,Eri Yoshida,Shuhei Naka,Hiroaki Inaba,Michiyo Matsumoto-Nakano
ABSTRACT Background Streptococcus mutans, a biofilm-forming bacterium, possesses several transporters that function as import/export molecules. Among them, the PII protein family is composed of members that regulate glutamine synthesis in bacterial species. Objective In this study, we characterized the function of the glutamine transporter in S. mutans MT8148. Methods The SMU.732 gene, corresponding
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A Dual Zinc plus Arginine formulation attenuates the pathogenic properties of Porphyromonas gingivalis and protects gingival keratinocyte barrier function in an in vitro model. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-08-04 Amel Ben Lagha,Ying Yang,Harsh M Trivedi,James G Masters,Daniel Grenier
ABSTRACT Background and objectives Porphyromonas gingivalis, a late colonizer of the periodontal biofilm, has been strongly associated with the chronic form of periodontitis. The aim of this study was to investigate the effects of a Dual Zinc plus Arginine formulation (aqueous solution and dentifrice) on the pathogenic properties of P. gingivalis and the barrier function of an in vitro gingival epithelium
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Porphyromonas gingivalis adopts intricate and unique molecular mechanisms to survive and persist within the host: a critical update. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-08-03 Aditi Chopra,Subraya G Bhat,Karthik Sivaraman
ABSTRACT Porphyromonas. gingivalis (P. gingivalis) is an obligate, asaccharolytic, gram-negative bacteria commonly associated with increased periodontal and systemic inflammation. P. gingivalis is known to survive and persist within the host tissues as it modulates the entire ecosystem by either engineering its environment or modifying the host’s immune response. It interacts with various host receptors
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Antiviral mouthwashes: possible benefit for COVID-19 with evidence-based approach. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-07-17 Mahdieh-Sadat Moosavi,Pouyan Aminishakib,Maryam Ansari
ABSTRACT Background The outbreak, and pandemic of COVID-19 causing widespread concerns in all health systems of countries. Virus-carrying aerosols can penetrate the healthy human body and lungs, resulting in rapid transmission. For the first time, in this evidence-based article, the effects of different types of mouthwashes to reduce the viral load were investigated. Also, another aim of this essay
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Mucus is more than just a physical barrier for trapping oral microorganisms. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-07-03 Ingar Olsen
ABSTRACT Mucus is thought to serve as a protective coating on wet epithelial surfaces. Recent research has shown that glycans, which are branched sugar molecules found in mucin, a part of mucus, can prevent bacteria from communicating with each other and forming biofilms. This could hinder microbes from causing infections. The present editorial, focusing on a paper by Wheeler et al. [1], published
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Longitudinal study on oral shedding of human betaherpesviruses 6 and 7 in renal transplant recipients reveals active replication. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-06-30 Jéssica Vasques Raposo,Dmitry José De Santana Sarmento,Rafaela Barbosa Da Silva Pinto,Amanda Oliveira Lopes,Marina Gallottini,Tânia Regina Tozetto-Mendoza,Paulo Henrique Braz-Silva,Vanessa Salete de Paula
ABSTRACT Backgroung Roseolovirus latency and persistence in salivary glands that are frequently reactivated after renal transplantation to cause infection have been reported. However, limited information is available on the persistence and excretion of HHV-6 and HHV-7 during and after transplant. Methods 32 renal transplant recipients were followed up before (T1) and after transplant (T2 and T3) and
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Carbon source utilization patterns in dental plaque and microbial responses to sucrose, lactose, and phenylalanine consumption in severe early childhood caries. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-06-23 Weihua Shi,Jing Tian,He Xu,Guiyan Wang,Qiong Zhou,Man Qin
ABSTRACT Background Severe early childhood caries (S-ECC) is mainly caused by the interaction of microbiota and environmental factors. However, the metabolic profiles of S-ECC microbial communities and the community-level microbial responses to carbohydrates and amino acids are poorly understood. Methods We collected supragingival plaques from 15 caries-free (CF) and 14 S-ECC children. Cultivation
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Phylogenetic diversity in fim and mfa gene clusters between Porphyromonas gingivalis and Porphyromonas gulae, as a potential cause of host specificity. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-06-19 Kaori Fujiwara-Takahashi,Takayasu Watanabe,Masahiro Shimogishi,Masaki Shibasaki,Makoto Umeda,Yuichi Izumi,Ichiro Nakagawa
ABSTRACT Background Periodontopathic bacteria Porphyromonas gingivalis in humans and Porphyromonas gulae in animals are phylogenetically close and commonly have FimA and Mfa1 fimbriae. However, little is known about how fimA and mfa1 are phylogenetically different between P. gingivalis and P. gulae. Here, we examined phylogenetic diversity in their fim and mfa gene clusters. Methods Twenty P. gulae
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A nanocarrier system that potentiates the effect of miconazole within different interkingdom biofilms. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-06-07 Laís Salomão Arias,Jason L Brown,Mark C Butcher,Christopher Delaney,Douglas Roberto Monteiro,Gordon Ramage
ABSTRACT Background Novel and new therapeutic strategies capable of enhancing the efficacy of existing antimicrobials is an attractive proposition to meet the needs of society. Objective This study aimed to evaluate the potentiating effect of a miconazole (MCZ) nanocarrier system, incorporated with iron oxide nanoparticles (IONPs) and chitosan (CS) (IONPs-CS-MCZ). This was tested on three representative
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A case study of salivary microbiome in smokers and non-smokers in Hungary: analysis by shotgun metagenome sequencing. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-06-07 Roland Wirth,Gergely Maróti,Róbert Mihók,Donát Simon-Fiala,Márk Antal,Bernadett Pap,Anett Demcsák,Janos Minarovits,Kornél L Kovács
ABSTRACT Objective To investigate the role of cigarette smoking in disease-development through altering the composition of the oral microbial community. Periodontitis and oral cancer are highly prevalent in Hungary; therefore, the salivary microbiome of smoker and non-smoker Hungarian adults was characterized. Methods Shotgun metagenome sequencing of salivary DNA samples from 22 individuals (11 non-smokers
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A review of co-culture models to study the oral microenvironment and disease. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-06-04 Sophie E Mountcastle,Sophie C Cox,Rachel L Sammons,Sara Jabbari,Richard M Shelton,Sarah A Kuehne
ABSTRACT Co-cultures allow for the study of cell–cell interactions between different eukaryotic species or with bacteria. Such an approach has enabled researchers to more closely mimic complex tissue structures. This review is focused on co-culture systems modelling the oral cavity, which have been used to evaluate this unique cellular environment and understand disease progression. Over time, these
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The use of bacterial DNA from saliva for the detection of GAS pharyngitis J. Oral Microbiol. (IF 3.939) Pub Date : 2020-05-27 Saar Hashavya; Naama Pines; Ayelet Gayego; Avi Schechter; Itai Gross; Alon Moses
ABSTRACT Background Acute tonsillitis is a very common medical condition. Despite different methods of detection, all tests are based on GAS sampling using a throat swab. However, obtaining the swab can elicit vomiting and is often accompanied by fear and apprehension in children. The aim of this study was to find a non-invasive method for the detection of GAS pharyngitis. Methods A classic throat
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Antifungal effects of Lactobacillus acidophilus and Lactobacillus plantarum against different oral Candida species isolated from HIV/ AIDS patients: an in vitro study. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-05-25 Samira Salari,Pooya Ghasemi Nejad Almani
ABSTRACT Oropharyngeal Candidiasis (OPC) is an opportunistic fungal infection occurring in immunocompromised patients such as HIV/AIDS. The purpose of this study was to evaluate the antifungal properties of Lactobacillus acidophilus and Lactobacillus plantarum on different Candida species isolated from oral cavity of HIV/AIDS patients compared to Fluconazole (FLC). In this study, the antifungal effects
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Incidence of infective endocarditis caused by viridans group streptococci in Sweden – effect of cessation of antibiotic prophylaxis in dentistry for risk individuals J. Oral Microbiol. (IF 3.939) Pub Date : 2020-05-23 Niko Vähäsarja; Bodil Lund; Anders Ternhag; Bengt Götrick; Lars Olaison; Margareta Hultin; Carina Krüger Weiner; Aron Naimi-Akbar
ABSTRACT Introduction In October 2012, the Swedish Medical Products Agency published new recommendations for the cessation of prophylactic antibiotics in dentistry for the prevention of infective endocarditis (IE). Previously, 2 g of amoxicillin per os would be administered 1 h before invasive dental procedures to patients with valve prosthesis, complicated heart valve disease, and to those with previous
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Microbiology of molar-incisor hypomineralization lesions. A pilot study. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-05-20 Miguel Hernández,Paloma Planells,Eva Martínez,Alex Mira,Miguel Carda-Diéguez
Objective: An insufficient mineralization (hypomineralization) in the teeth during the maturation stage of amelogenesis cause defects in 3-44% of children. Here, we describe for the first time the microbiota associated with these defects and compared it to healthy teeth within the same subjects. Methods: Supragingival dental plaque was sampled from healthy and affected teeth from 25 children with molar-incisor
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Microbiology of molar–incisor hypomineralization lesions. A pilot study J. Oral Microbiol. (IF 3.939) Pub Date : 2020-05-20 Miguel Hernández; Paloma Planells; Eva Martínez; Alex Mira; Miguel Carda-Diéguez
ABSTRACT Objective: An insufficient mineralization (hypomineralization) in the teeth during the maturation stage of amelogenesis cause defects in 3–44% of children. Here, we describe for the first time the microbiota associated with these defects and compared it to healthy teeth within the same subjects. Methods: Supragingival dental plaque was sampled from healthy and affected teeth from 25 children
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Dental aerosols: microbial composition and spatial distribution. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-05-13 C Zemouri,C M C Volgenant,M J Buijs,W Crielaard,N A M Rosema,B W Brandt,A M G A Laheij,J J De Soet
Background: High-speed dental instruments produce aerosols, which can contribute to the transmission of pathogenic microorganisms. The aim of this study is to describe the microbial load and - composition and spatial distribution of aerosols in dental clinics. Methods: In four dental clinics active and passive sampling methods were used before, during and after treatment and at different locations
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Lasting Gammaproteobacteria profile changes characterized hematological cancer patients who developed oral mucositis following conditioning therapy. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-05-13 Jean-Luc C Mougeot,Micaela F Beckman,Craig B Stevens,Kathryn G Almon,Darla S Morton,Inger Von Bültzingslöwen,Michael T Brennan,Farah Bahrani Mougeot
ABSTRACT Background: Oral mucositis (OM) is a common side effect of conditioning therapy implemented before hematopoietic stem cell transplantation (HSCT). The role of oral microbiome in OM is not fully elucidated. Objective: To determine oral microbiome profile changes post-conditioning in HSCT patients who developed moderate OM, or mild to no OM. Design: Patient groups were: Muc0-1 with OM-score = 0–1
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Dental aerosols: microbial composition and spatial distribution J. Oral Microbiol. (IF 3.939) Pub Date : 2020-05-13 C. Zemouri; C.M.C. Volgenant; M.J. Buijs; W. Crielaard; N.A.M. Rosema; B.W. Brandt; A.M.G.A. Laheij; J.J. De Soet
ABSTRACT Background: High-speed dental instruments produce aerosols, which can contribute to the transmission of pathogenic microorganisms. The aim of this study is to describe the microbial load and – composition and spatial distribution of aerosols in dental clinics. Methods: In four dental clinics active and passive sampling methods were used before, during and after treatment and at different locations
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Outer membrane vesicle-mediated serum protection in Aggregatibacter actinomycetemcomitans. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-04-08 Mark Lindholm,Marjut Metsäniitty,Elisabeth Granström,Jan Oscarsson
Aggregatibacter actinomycetemcomitans belongs to the HACEK group of fastidious Gram-negative organisms, a recognized cause of infective endocarditis. A. actinomycetemcomitans is also implicated in periodontitis, with rapid progress in adolescents. We recently demonstrated that the major outer membrane protein, OmpA1 was critical for serum survival of the A. actinomycetemcomitans serotype a model strain
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Co-occurrence of yeast, streptococci, dental decay, and gingivitis in the post-partum period: results of a longitudinal study. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-04-15 Kirtana Ramadugu,Freida Blostein,Deesha Bhaumik,Wenwen Jiang,Elyse Davis,Elizabeth Salzman,Usha Srinivasan,Carl F Marrs,Katherine Neiswanger,Daniel W McNeil,Mary L Marazita,Betsy Foxman
Objective: The interactions between yeast and streptococci species that lead to dental decay and gingivitis are poorly understood. Our study describes these associations among a cohort of 101 post-partum women enrolled in the Center for Oral Health Research in Appalachia, 2012-2013. Methods: All eligible women without dental caries were included (n = 21) and the remainder were randomly sampled to represent
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Acetaldehyde production by Rothia mucilaginosa isolates from patients with oral leukoplakia. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-03-21 Abdrazak Amer,Aine Whelan,Nezar N Al-Hebshi,Claire M Healy,Gary P Moran
Rothia mucilaginosa has been found at high abundance on oral leukoplakia (OLK). The ability of clinical isolates to produce acetaldehyde (ACH) from ethanol has not been investigated. The objective of the current study was to determine the capacity of R. mucilaginosa isolates recovered from OLK to generate ACH. Analysis of R. mucilaginosa genomes (n = 70) shows that this species does not normally encode
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Porphyromonas gingivalis W83 traffics via ICAM1 in microvascular endothelial cells and alters capillary organization in vivo. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-03-26 L Reyes,H Getachew,W A Dunn,A Progulske-Fox
Objective: Microvascular dysfunction is a feature of periodontal disease. P. gingivalis, one of the most common oral bacteria present in gingival tissue biofilms, has also been identified in the gingival capillaries of patients with chronic periodontitis. We sought to determine the effect of P. gingivalis W83 infection on microvascular endothelium in vivo and in vitro. Methods and Results: Interdental
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The relationship between cigarette smoking and the tongue microbiome in an East Asian population. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-03-25 Noriaki Sato,Masanori Kakuta,Eiichiro Uchino,Takanori Hasegawa,Ryosuke Kojima,Wataru Kobayashi,Kaori Sawada,Yoshihiro Tamura,Itoyo Tokuda,Seiya Imoto,Shigeyuki Nakaji,Koichi Murashita,Motoko Yanagita,Yasushi Okuno
Background: The oral microbiome, which consists of various habitats, has been shown to be influenced by smoking. However, differences in the tongue microbiomes of current and former smokers, as well as their resultant functional consequences, have rarely been investigated in East Asian populations. Methods: We used 16S rRNA amplicon sequencing of tongue-coating samples obtained from East Asian subjects
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Innovative application of nested PCR for detection of Porphyromonas gingivalis in human highly calcified atherothrombotic plaques. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-04-08 A Brun,H Rangé,B Prouvost,M Mazighi,Y Kapila,P Bouchard,J B Michel
Atherothrombosis, leading to stroke and myocardial infarction, is responsible for most of the deaths in the world. An increased risk of atherothrombotic vascular events has been reported in patients with periodontitis. Periodontitis is a chronic multifactorial inflammatory disease, which involves a dysbiotic microbiota, and leads to a progressive destruction of the tooth-supporting apparatus. Transcient
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The microbial abundance dynamics of the paediatric oral cavity before and after sleep. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-03-30 Jessica A P Carlson-Jones,Anna Kontos,Declan Kennedy,James Martin,Kurt Lushington,Jody McKerral,James S Paterson,Renee J Smith,Lisa M Dann,Peter Speck,James G Mitchell
Objective: Microhabitats in the oral cavity differ in microbial taxonomy. However, abundance variations of bacterial and viral communities within these microhabitats are not fully understood. Aims and Hypothesis: To assess the spatial distribution and dynamics of the microbial abundances within 6 microhabitats of the oral cavity before and after sleep. We hypothesise that the abundance distributions
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Supragingival mycobiome and inter-kingdom interactions in dental caries J. Oral Microbiol. (IF 3.939) Pub Date : 2020-02-19 Divyashri Baraniya; Tsute Chen; Anubhav Nahar; Fadhl Alakwaa; Jennifer Hill; Marisol Tellez; Amid Ismail; Sumant Puri; Nezar Noor Al-Hebshi
ABSTRACT Background: Recent studies have reveled the presence of a complex fungal community (mycobiome) in the oral cavity. However, the role of oral mycobiome in dental caries and its interaction with caries-associated bacteria is not yet clear. Methods: Whole-mouth supragingival plaque samples from 30 children (6–10 years old) with no caries, early caries, or advanced caries were sequenced for internal
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Candida biome of severe early childhood caries (S-ECC) and its cariogenic virulence traits. J. Oral Microbiol. (IF 3.939) Pub Date : 2020-02-05 Kausar Sadia Fakhruddin,Lakshman Perera Samaranayake,Hiroshi Egusa,Hien Chi Ngo,Chamila Panduwawala,Thenmozhi Venkatachalam,Allagappan Kumarappan,Siripen Pesee
The protected niche of deep-caries lesions is a distinctive ecosystem. We assessed the Candida biome and its cariogenic traits from dentin samples of 50 children with severe-early childhood caries (S-ECC). Asymptomatic, primary molars belonging to International Caries Detection and Assessment-ICDAS caries-code 5 and 6 were analyzed, and C. albicans (10-isolates), C. tropicalis (10), C. krusei (10)