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Colacogloea armeniacae sp. nov., a novel pucciniomycetous yeast species isolated from apricots Mycoscience (IF 1.172) Pub Date : 2020-08-31 Gui-Shuang Wang; Yong Sun; Wangmu; Qi-Ming Wang
A survey of yeasts associated with apricots was carried out in May 2019. Two strains isolated from two fresh apricots were identified as a novel species of the genus Colacogloea based on a multi locus phylogenetic analysis, and physiological and biochemical tests. The two strains differed from any hitherto known Colacogloea species by at least 18 nucleotide substitutions (3%) in the D1/D2 domains of
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Hyphoderma fissuratum and H. mopanshanense spp. nov. (Polyporales) from southern China Mycoscience (IF 1.172) Pub Date : 2020-08-20 Xiang Ma; Ruo-Xia Huang; Ying Zhang; Chang-Lin Zhao
Two new species, Hyphoderma fissuratum and H. mopanshanense spp. nov., are proposed based on morphological and molecular evidences. Hyphoderma fissuratum is characterized by resupinate basidiomata with cracking hymenial surface, a monomitic hyphal system with generative hyphae bearing clamp connections, IKI–, CB–, and cylindrical, colorless, thin-walled, smooth basidiospores measuring (8.5–10.3 × 3–4 μm)
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Two new deer truffle species, Elaphomyces marmoratus and Elaphomyces fuscus spp. nov., from a secondary forest in Japan Mycoscience (IF 1.172) Pub Date : 2020-08-13 Makoto Shirakawa; Megumi Tanaka
We describe two new species of deer truffle, Elaphomyces marmoratus and E. fuscus spp. nov., collected from a secondary forest dominated by Quercus serrata, in Tokyo, Japan. Both species have morphological similarities to E. muricatus and E. granulatus; however, the former has a gleba without a purplish color and the latter has reticulated ascospores more closely resembling E. asperulus. Molecular
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Abundance and phenology of macrofungal fruiting bodies in central and northern Japan Mycoscience (IF 1.172) Pub Date : 2020-08-10 Nobuko Tuno; Daisuke Akaishi; Masahito T. Kimura
To understand how ectomycorrhizal (ECM), wood-decomposing (WDC) and litter-decomposing (LDC) fungi differ in abundance and fruiting season, fruiting-body production was monitored by counting their number and/or measuring their biomass in deciduous broad-leaved and coniferous forests in Ishikawa (central Japan) and Hokkaido (northern Japan). ECM fungi were dominant in forests of both types in Ishikawa
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Athelia termitophila sp. nov. is the teleomorph of the termite ball fungus Fibularhizoctonia sp. Mycoscience (IF 1.172) Pub Date : 2020-08-07 Nitaro Maekawa; Hiroaki Yokoi; Kozue Sotome; Kenji Matsuura; Chihiro Tanaka; Naoki Endo; Akira Nakagiri; Shuji Ushijima
A new species of Athelia, A. termitophila, from Japan is described and illustrated on the basis of morphological and phylogenetic analyses. Basidiomes of this species are characterized by having hyphae sometimes with clamp connections at the septa, basidia without clamp connections at the basal septa, and ellipsoid to ovoid basidiospores measuring 4.5–6 × 3–4.5 μm. In culture, mycelia produce pale
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Taxonomic and nomenclatural changes in euantennariaceous sooty moulds: Ten new combinations in Euantennaria for species of Antennatula Mycoscience (IF 1.172) Pub Date : 2020-07-23 Junta Sugiyama; Tsuyoshi Hosoya
The correct type species of Antennatula Fr. ex F. Strauss 1850 was quite recently considered to be Antennularia ericophila (Link) Höhn., not Antennatula pinophila (Nees) F. Strauss, and currently the illegitimate name Antennatula is regarded as a synonym of Metacapnodium Speg. 1918 (Metacapnodiaceae). Among Euantennaria Speg. 1918 and competing synonymous generic names Aithalomyces Woron. 1926, Phaeocapnis
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Mallocybe velutina (Agaricales, Inocybaceae), a new species from Pakistan Mycoscience (IF 1.172) Pub Date : 2020-07-13 Malka Saba; Abdul Nasir Khalid
A new species of Mallocybe is described and illustrated based on material collected near the vicinity of Pinus wallichiana in mixed conifer forests in district Abbottabad, Khyber Pakhtoonkhaw, Pakistan. Mallocybe velutina is recognized by the presence of moderate yellow to light yellow pileal margin with deep yellow or fulvous pileus center; strikingly velvety pileus surface; subdistant moderate yellow
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A new species and two new Chinese records of Ochroconis from sugarcane and banana rhizosphere in Guangxi, China Mycoscience (IF 1.172) Pub Date : 2020-07-09 Yanlu Chen; Ling Xie; Yanyan Long; Yan Zhang; Bixia Qin; Shitong Liao; Qin Su; Bin Liu
Ochroconis guangxiensis isolated from sugarcane and banana rhizosphere, was described as a new species based on morphological characteristics and phylogenetic analysis using sequence data of the nuclear small subunit rRNA gene (SSU), internal transcribed spacer (ITS) region and large subunit (LSU) rRNA gene. Taxonomic and phylogenetic remarks are also provided for O. minima and O. ramosa. The latter
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Tricholomopsis rubroaurantiaca, a new species of Tricholomataceae from southern China Mycoscience (IF 1.172) Pub Date : 2020-06-27 Md. Iqbal Hosen; Jun-Yan Xu; Ting Li; Genevieve Gates; Tai-Hui Li
Species of Tricholomopsis are known to be lignicolous and to cause white rot. Several collections of Tricholomopsis that grow near bamboo were sampled from southern China and morphologically could not be assigned to any known species of Tricholomopsis. Furthermore, phylogenetic analyses inferred from nuclear ribosomal internal transcribed spacer region (ITS) and the D1-D2 domains of the 28S rDNA large
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Aspergillus jilinensis sp. nov. And its thermostable alkaline enzymes evaluation Mycoscience (IF 1.172) Pub Date : 2020-06-11 Panpan Huang, Xianzhi Jiang, Bing Wu, Jingzu Sun
Aspergillus comprises a diverse group of species, which have a ubiquitous distribution and occur on decaying vegetation, soil, and dust. During an investigation of the diversity of alkali-tolerant or alkalophilic fungi in China, two isolates producing columnar conidial heads with globose conidia belonging to Aspergillus were isolated from the soda soil and named as Aspergillus jilinensis. Its phylogenetic
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Characterization of Japanese Lactarius section deliciosi ectomycorrhizae: Toward the use of mycorrhizae for taxonomy and expansion of available cultures Mycoscience (IF 1.172) Pub Date : 2020-06-09 Naoki Endo; Tatsuki Yamamoto; Ryo Sugawara; Kozue Sotome; Nitaro Maekawa; Akira Nakagiri
We described the ectomycorrhizae (ECM) of the Japanese Lactarius section Deliciosi species L. akahatsu, L. hatsudake, L. laeticolor, and L. subindigo with the aim of promoting the use of mycorrhizae for taxonomy and expansion of available cultures. Lactarius tottoriensis (sect. Lactarius) was also included as a comparator. The ECM of the four species of sect. Deliciosi showed orange laticifers and
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A new lichen-forming fungus, Orientophila corticola, from South Korea, with a key to the genus Mycoscience (IF 1.172) Pub Date : 2020-06-08 Beeyoung Gun Lee, Jae-Seoun Hur
Orientophila corticola is described as a new lichen-forming fungus from South Korea. The new species is distinguishable from other Orientophila species by the substrate preference to tree barks and the habitat specificity to inland areas. Molecular analyses applying internal transcribed spacer (ITS) and mitochondrial small subunit (mtSSU) sequences clearly classify O. corticola as a distinct species
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Metapochonia hahajimaensis (Clavicipitaceae, Hypocreales), a new species from soil in Hahajima Island, Tokyo, Japan Mycoscience (IF 1.172) Pub Date : 2020-06-04 Naozumi Kondo; Toshiyuki Tokiwa; Kotaro Sato; Satoshi Ōmura; Kenichi Nonaka
A new species, Metapochonia hahajimaensis is proposed. Three isolates were obtained from soil samples collected from Hahajima, Ogasawara Islands, Tokyo, Japan. Using a combination of morphological characteristics and multigene (ITS, SSU, LSU, TEF) phylogenics, the isolates were identified as a Metapochonia species which were previously undescribed. The new species is distinguished from the closely-related
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Physisporinus is an important mycorrhizal partner for mycoheterotrophic plants: Identification of mycorrhizal fungi of three Yoania species Mycoscience (IF 1.172) Pub Date : 2020-05-26 Yumi Yamashita, Akihiko Kinoshita, Takahiro Yagame, Yuki Ogura-Tsujita, Jun Yokoyama, Tomohisa Yukawa
Improved understanding of mycorrhizal diversity in mycoheterotrophic (MH) plants is a key element of studies that investigate their evolution. MH plants are completely dependent on their mycorrhizal fungi for carbon. Mycorrhizal fungi of the MH genus Yoania (Orchidaceae), which is distributed in East Asia, have yet to be identified. We identified the mycobionts of three Japanese Yoania species, Y. amagiensis
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Grosmannia tibetensis, a new ophiostomatoid fungus associated with Orthotomicus sp. (Coleoptera) in Tibetan subalpine forests Mycoscience (IF 1.172) Pub Date : 2020-05-24 Zheng Wang; Ya Liu; Tiantian Wang; Cony Decock; Biao Chu; Qinglian Zheng; Quan Lu; Xingyao Zhang
Few ophiostomatoid fungi have been reported from the margin of the Tibetan Plateau and none have been found in the central portion of the region. In a survey of ophiostomatoid fungi associated with spruce bark beetles in Tibetan subalpine forests, numerous strains of Leptographium s. l. (Ophiostomataceae) were isolated from Orthotomicus sp. (Coleoptera: Scolytinae) and its galleries infesting Picea
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Systematics, ecology, and application of Helotiales: Recent progress and future perspectives for research with special emphasis activities within Japan Mycoscience (IF 1.172) Pub Date : 2020-05-18 Tsuyoshi Hosoya
Helotiales is one of the most diverse groups of apothecial ascomycetes, including 3000–4000 taxa. Recent progress in the systematics, ecology, and their applications through research is herein reviewed based on the experiences of the author with a special emphasis on activities in Japan. In the past 30 y, more than 50 helotialean taxa have been added to the mycobiota of Japan, including new taxa. With
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Transcription analysis of Ganoderma lucidum reveals candidate genes and pathways in response to excess exogenous indoleacetic acid (IAA) Mycoscience (IF 1.172) Pub Date : 2020-05-12 Weiwei Zhang, Jin Lan, Xiangdong Chen
Known as auxin which regulate the plant growth, indoleacetic acid (IAA) was also found to be synthesized in basidiomycetes Ganoderma lucidum in our previously work. In the present study, the effect of excess exogenous IAA on mycelium of G. lucidum was determined. Result shows that the mycelium growth was significantly inhibited under 10 μM IAA treatment. The transcripts of both the IAA treated group
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First report of heteroecism in Stereostratum corticioides, the causal agent of bamboo culm rust Mycoscience (IF 1.172) Pub Date : 2020-05-06 Izumi Okane, Yuho Ando, Yuichi Yamaoka, Mitsuteru Akiba, Shinya Kubo
An aecidium-type rust fungus, which causes rust disease in Choerospondias axillaris (Anacardiaceae), was found on Kyusyu Is., Japan. A sequence analysis of genomic ribosomal DNA showed that this rust fungus genetically matches Stereostratum corticioides, a causal agent of culm rust disease of bamboos and broadleaf bamboos. Inoculation experiments and further nucleotide sequence analyses that included
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A new species of Leptobacillim, L. symbioticum, isolated from mites and sori of soybean rust Mycoscience (IF 1.172) Pub Date : 2020-04-18 Izumi Okane, Kenichi Nonaka, Yuko Kurihara, Junichi P. Abe, Yuichi Yamaoka
A verticillium-like fungus forming a whitish colony and mainly solitary phialides that produced fusiform to cylindrical conidia in chains was isolated from uredinia of Phakopsora pachyrhizi, a causal agent of soybean rust. In addition, two similar looking isolates were obtained from Prostigmata mites. Our taxonomic study based on morphology and phylogenetic analysis using ITS rDNA and LSU rDNA D1/D2
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Effects of imidazole-4-carboxamide and 2-azahypoxanthine on the growth and ectomycorrhizal colonization of Pinus densiflora seedlings inoculated with Tricholoma matsutake Mycoscience (IF 1.172) Pub Date : 2020-04-14 Shota Nakano, Takashi Yamanaka, Hirokazu Kawagishi
Imidazole-4-carboxamide (ICA) and 2-azahypoxanthine (AHX) obtained from Lepista sordida inhibit and promote the growth of herbaceous plants, respectively. In this study, we examined the effects of these compounds on the growth and ectomycorrhizal (EM) colonization of Pinus densiflora seedlings inoculated with Tricholoma matsutake that forms EM associations with pines. The EM colonization by T. matsutake
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Non-destructive analysis of the moisture content in shiitake mushrooms (Lentinula edodes) using near-infrared imaging at 1450 nm Mycoscience (IF 1.172) Pub Date : 2020-04-14 Kazuma Shinoda, Naotake Konno, Tomohiro Suzuki
Moisture content (MC) is an important factor in the freshness of shiitake mushrooms (Lentinula edodes). In this study, we developed a simple prediction method for MC of shiitake mushrooms using near-infrared (NIR) imaging at 1450 nm, as the band at 1450 nm is strongly correlated with OH groups. First, a NIR image was captured by an InGaAs sensor with a bandpass filter of 1450 nm; the captured image
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The proportions of ectomycorrhizal roots are varied depending on the different host plant compositions in Scottish arctic/alpine coastal relict vegetation Mycoscience (IF 1.172) Pub Date : 2020-04-07 Hironari Izumi
The relative abundance of ectomycorrhizal (ECM) roots in pure and mixed stand compositions of Dryas octopetala, Salix repens and Arctostaphylos uva-ursi grown in Scottish coastal arctic/alpine relict habitat was described through ECM morphotyping and comparisons of fungal internal transcribed spacer sequences. Neither specific ECM morphotypes nor the morphotype communities were found in association
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Cystomyces antheropori, a new rust fungus on Antheroporum glaucum from Thailand Mycoscience (IF 1.172) Pub Date : 2020-04-05 Chanjira Ayawong, Jintana Unartngam, Pornpawee Thiwatwaranikul, Izumi Okane, Yoshitaka Ono
A rust fungus was found on leaves of Antheroporum glaucum (Fabaceae, Faboideae) in Provinces of Petchaburi and Ratchaburi, western Thailand. No rust fungus was previously reported on this legume tree species. The new rust fungus was apparently microcyclic, producing telia surrounding dense aggregates of spermogonia, on the abaxial leaf surface. Teliospores were produced by sympodial proliferation from
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Morphological and molecular identification of two new species of Tubulicrinis (Hymenochaetaceae, Hymenochaetales) from southern China Mycoscience (IF 1.172) Pub Date : 2020-04-03 Xiao He, Zheng-Jun Shi, Chang-Lin Zhao
Two new wood-inhabiting fungal species, Tubulicrinis xantha and T. yunnanensis spp. nov., are described based on morphological and molecular characters. Tubulicrinis xantha is characterized by resupinate, furfuraceous basidiomata with buff to yellowish hymenial surface, amyloid lyocystidia and cylindrical to allantoid, hyaline, thin-walled, smooth basidiospores (5.3–6.3 × 1.2–1.6 μm). Tubulicrinis
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Hirticrusta gen. nov. segregated from Neofomitella in Polyporaceae (Polyporales) Mycoscience (IF 1.172) Pub Date : 2020-03-31 Toshinori Matozaki, Tsutomu Hattori, Nitaro Maekawa, Akira Nakagiri, Noemia Kazue Ishikawa, Kozue Sotome
Taxonomic studies including morphological observations and phylogenetic analyses were conducted on Japanese “uragin-take”, an unidentified species from Amazonia, Brazil and their allies. Phylogenetic analyses using ITS, nrLSU and RPB2 regions revealed that “uragin-take”, Neofomitella polyzonata and the unidentified species formed a monophyletic clade separate from the clade including the other four
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Ravenelia species (Pucciniales) newly found in Thailand with their additional host and life cycle stage Mycoscience (IF 1.172) Pub Date : 2020-03-20 Chanjira Ayawong, Jintana Unartngam, Wen-Hsin Chung, Yoshitaka Ono
Three poorly known species of Ravenelia with limited geographic distribution and one widespread species parasitizing leguminous trees (Fabaceae) were newly found in Thailand. Ravenelia odoratissimae occurred on Albizia odoratissima; R. ornata on Abrus pulchellus, Ab. precatorius, and Abrus sp.; R. parasnathii on Acacia comosa and an unidentified Acacia species; and R. tandonii on Acacia catechu. Acacia
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Phylogenetic overview of Erysiphaceae based on nrDNA and MCM7 sequences Mycoscience (IF 1.172) Pub Date : 2020-03-20 Takashi Shirouzu, Susumu Takamatsu, Akira Hashimoto, Jamjan Meeboon, Moriya Ohkuma
The classification system and evolutionary history of Erysiphaceae have been studied based on the results of molecular phylogenetic analyses. However, the sequence data used for these phylogenetic estimations have been limited to the nrDNA of ca., 50 taxa, and the relationships among higher taxonomic groups are not well understood. To provide a phylogenetic overview of Erysiphaceae, we performed phylogenetic
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Two new Microascus species with spinous conidia isolated from pig farm soils in China Mycoscience (IF 1.172) Pub Date : 2020-03-19 Bingda Sun, Yuguang Zhou, Amanda J. Chen
Two scopulariopsis-like fungi were isolated when surveying fungi in pig farm soils in China. Sexual structures of these fungi were not observed and their conidia had spinous walls. Phylogenetic analysis based on nucleotide sequences of the internal transcribed spacer 1 and 2 and intervening 5.8S ribosomal RNA gene (ITS), large subunit ribosomal RNA gene (LSU), beta-tubulin (tub2) and translation elongation
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Multiclavula petricola sp. nov. (Cantharellales, Basidiomycota), a new clavarioid and lichenized fungus growing on rocks Mycoscience (IF 1.172) Pub Date : 2020-03-15 Hiroshi Masumoto, Yousuke Degawa
Multiclavula petricola is described as a new species based on morphological characters, its unique habitat on rocks, and the result of molecular phylogenetic analyses of the sequence of internal transcribed spacer of the nuclear ribosomal DNA. The fruiting bodies of M. petricola were dispersed on wet rock surface with numerous lichenized globules. The globular thallus was organized by several green
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Isaria macroscyticola, a rare entomopathogenic species on Cydnidae (Hemiptera), is a synnematous form and synonym of Purpureocillium lilacinum (Ophiocordycipitaceae) Mycoscience (IF 1.172) Pub Date : 2020-03-06 Kohei Yamamoto, Mamoru Yasuda, Muneyuki Ohmae, Hiroki Sato, Takamichi Orihara
Isaria macroscyticola is a hypocrealean pathogen of Cydnidae first described by Dr. Yosio Kobayasi in 1941. Since then, records of this species have been limited, and therefore no cultures have been established and its phylogenetic position is unknown. Recently, synnemata on Macroscytus japonensis were collected from Kanagawa Prefecture, Japan. These were identified as I. macroscyticola based on the
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Natural roles of nonribosomal peptide metabolites in fungi Mycoscience (IF 1.172) Pub Date : 2020-03-05 Shinichi Oide, B. Gillian Turgeon
Over 90 years have passed since Alexander Fleming's discovery of penicillin, the first recognized, naturally occurring antibiotic. Penicillin is a representative of a group of metabolites produced by large multienzyme complexes [nonribosomal peptide synthetases (NRPSs)] in a ribosome-independent fashion. Nonribosomal peptides (NRPs) are structurally diverse metabolites produced almost exclusively by
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Phylogeny and taxonomic revision of the genus Candelabrum, aero-aquatic fungi Mycoscience (IF 1.172) Pub Date : 2020-02-28 Kaoru Yamaguchi; Charuwan Chuaseeharonnachai; Seppo Huhtinen; Yasuhisa Tsurumi; Veera Sri-Indrasutdhi; Nattawut Boonyuen; Izumi Okane; Tsuyoshi Hosoya; Akira Nakagiri
The genus Candelabrum, an aero-aquatic fungus, produces chandelier-shaped conidia that can float on the water. Seven species have been described in the genus. Our phylogenetic analysis based on the D1/D2 regions of the large subunit rDNA (LSU) revealed that the genus Candelabrum contains two separate lineages, C. spinulosum group (C. clathrosphaeroides, C. desmidiaceum, C. japonense, and C. spinulosum
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Syncephalastrum contaminatum, a new species in the Mucorales from Australia Mycoscience (IF 1.172) Pub Date : 2020-02-22 Andrew S. Urquhart, Alexander Idnurm
A new species is described in the Mucorales family Syncephalastraceae: Syncephalastrum contaminatum, isolated as an in vitro culture from a laboratory contaminant. The species has variable copies of the internal transcribed spacer (ITS) regions, requiring cloning of these regions prior to Sanger sequencing before subsequent use in phylogenetic comparisons with other fungi. The genome of the strain
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Simplicillium spumae (Cordycipitaceae, Hypocreales), a new hyphomycetes from aquarium foam in Japan Mycoscience (IF 1.172) Pub Date : 2020-02-16 Naozumi Kondo, Hiromu Iwasaki, Toshiyuki Tokiwa, Satoshi Ōmura, Kenichi Nonaka
Five Simplicillium isolates were obtained from foam generated in an aquarium in Minato-ku, Tokyo, Japan. Using a combination of micro-morphological characteristics and multigene (ITS, SSU, LSU, TEF) phylogenics, the isolates were identified as S. subtropicum and a new species which were previously undescribed. Simplicillium spumae, which is a new species, differs from known Simplicillium species in
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Spore formation and karyological characterization of basidiosporogenesis, meiosis, post-meiotic and nuclear migration mitosis in Coprinus comatus Mycoscience (IF 1.172) Pub Date : 2020-02-06 Fu-Chia Chen, Norihiro Shimomura, Takeshi Yamaguchi, Tadanori Aimi
In Coprinus comatus, we sampled and observed different maturity stages of basidium to find the possible causes of binucleate basidiospores by microscopy using the HCI-Giemsa and DAPI staining methods. In basidium, following karyogamy, a single nucleolus was observed. We found evidence suggesting post-meiotic mitosis following nuclear migration in the spores. Post-meiotic mitosis occurred in the basidiospores
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Purification and characterization of low molecular weight alkali stable xylanase from Neosartorya spinosa UZ-2-11 Mycoscience (IF 1.172) Pub Date : 2020-01-31 Chollada Somboon, Santhaya Boonrung, Somporn Katekaew, Jindarat Ekprasert, Tadanori Aimi, Sophon Boonlue
Alkaliphilic xylanase from Neosartorya spinosa UZ-2-11 was purified using a three-step of purification scheme of ammonium sulphate precipitation followed by Sephadex G-100 gel filtration and DEAE-cellulose ion-exchange chromatography, and compared its properties with N. tatenoi KKU-CLB-3-2-4-1 of our previous report. The purified xylanase from N. spinosa UZ-2-11 exhibited maximum activity at pH 9.0
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Assessment of waste frying oil transesterification capacities of local isolated Aspergilli species and mutants Mycoscience (IF 1.172) Pub Date : 2020-01-28 Nadeem I. Elhussiny, Abd El-Nasser A. Khattab, Heba A. El-Refai, Sayeda S. Mohamed, Yousseria M. Shetaia, Hala A. Amin
Biodiesel (fatty acids methyl esters, FAME) has attracted considerable attention as an environmentally and eco-friendly alternative for diesel engines. This research manipulates the use of Aspergillus whole-cell lipase as a biocatalyst for biodiesel production from waste frying oil (WFO). A total of 17 isolates of Aspergillus species screened for lipase and esterase production abilities. Qualitatively
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Advances in the cultivation of the highly-prized ectomycorrhizal mushroom Tricholoma matsutake Mycoscience (IF 1.172) Pub Date : 2020-01-22 Takashi Yamanaka, Akiyoshi Yamada, Hitoshi Furukawa
Matsutake, the fruiting body of Tricholoma matsutake, is among the most economically important edible ectomycorrhizal (EM) mushrooms worldwide. This EM fungus develops “shiros”, which are mycelial aggregations that develop in association with the roots of EM coniferous trees and soil particles in well-drained and nutrient-poor forest soil. The fruiting bodies occur on the periphery of the outward-growing
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A new species of Pithoascus and first report of this genus as endophyte associated with Ferula ovina Mycoscience (IF 1.172) Pub Date : 2020-01-20 Zahra Tazik, Kamran Rahnama, Mehrdad Iranshahi, James Francis White, Hassan Soltanloo
A newly described species of Pithoascus from roots of Ferula ovina differs from other Pithoascus species by producing larger ascomata than all described species except P. exsertus. The shape of its ascospores is similar to that of P. lunatus, but differs from it by having an asexual state. This new species differs from P. ater by having a sexual state. Phylogenetic analyses based on concatenated ITS
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Influence of pH on in vitro mycelial growth in three Japanese truffle species: Tuber japonicum, T. himalayense, and T. longispinosum Mycoscience (IF 1.172) Pub Date : 2019-12-19 Shota Nakano, Akihiko Kinoshita, Keisuke Obase, Noritaka Nakamura, Hitomi Furusawa, Kyotaro Noguchi, Takashi Yamanaka
Truffle species indigenous to Japan include a white-colored truffle Tuber japonicum and two black-colored truffles, T. himalayense and T. longispinosum. As the fruiting bodies of these Tuber species are promising edible, studies on the artificial cultivation for these truffles are currently underway in Japan. In the present study, we investigated the influence of pH on in vitro mycelial growth in these
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Ochropsora staphyleae, a new rust pathogen of Japanese bladdernut, found in central Japan Mycoscience (IF 1.172) Pub Date : 2019-11-29 Yoshitaka Ono, Sinchai Chatasiri, Eiji Tanaka
Japanese bladdernut shrub, Staphylea bumalda (Staphyleaceae), was found infected by a fungus in Prefectures of Ibaraki and Tochigi, central Japan. The fungus produced telia beneath the host epidermis and basidiospores on metabasidia emerging from the sori on the abaxial leaf surface. Probasidia were single-celled, sessile, short-cylindrical, thin-walled, and laterally free. A four-celled metabasidium
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Determination of volatile organic compounds in the stinkhorn fungus Pseudocolus fusiformis in different stages of fruiting body formation Mycoscience (IF 1.172) Pub Date : 2019-11-07 Pattana Kakumyan, Nakarin Suwannarach, Jaturong Kumla, Natsaran Saichana, Saisamorn Lumyong, Kenji Matsui
A stinkhorn fungus was collected from the mountainous area of Yoshida campus, Yamaguchi University, Japan. Morphological characterization and similarity of large subunit ribosomal DNA sequences identified the fungus as Pseudocolus fusiformis. MonoTrap™ was combined with gas chromatography-mass spectrometry (GC-MS) to identify volatile organic compounds (VOCs) emitted from the fungus harvested at different
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Aspergillus yunnanensis, a new and rare species in the Aspergillus section Jani Mycoscience (IF 1.172) Pub Date : 2019-11-01 Wenjiao Cai, Panpan Huang, Ying Yan, Bingda Sun, Xianzhi Jiang, Amanda J. Chen
Aspergillus is a monophyletic genus comprising the subgenera Aspergillus, Circumdati, Cremei, Fumigati, Nidulantes and Polypaecilum. The subgenus Circumdati contains many economically important species and mycotoxin producers. Section Jani was recently introduced with morphological and molecular support. In the present study, two strains isolated from farmland soil were assigned in section Jani based
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Study of the intracellular xylanolytic activity of the phytopathogenic fungus Sporisorium reilianum Mycoscience (IF 1.172) Pub Date : 2019-10-26 Joany Pérez-Rodríguez, Alejandro Téllez-Jurado, Jorge Álvarez-Cervantes, J. Antonio Ibarra, Blanca Estela Jaramillo-Loranca, Miguel Angel Anducho-Reyes, Yuridia Mercado-Flores
The present study demonstrates that Sporisorium reilianum, a phytopathogenic fungus of corn, produces intracellular xylanolytic activity during submerged fermentation. Production reached its highest levels in a medium containing glucose, corn hemicellulose and yeast extract. An intracellular xylanase was purified by a process that included precipitation with ammonium sulfate, ion exchange chromatography
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An adaptor protein BmSte50 interacts with BmSte11 MAPKKK and is involved in host infection, conidiation, melanization, and sexual development in Bipolaris maydis Mycoscience (IF 1.172) Pub Date : 2019-10-23 Takuya Sumita, Kosuke Izumitsu, Sae Shigeyoshi, Shunsuke Gotoh, Hiroki Yoshida, Kenya Tsuji, Hiroshi Yoshida, Yuki Kitade, Chihiro Tanaka
A mitogen-activated protein kinase (MAPK) signaling pathway regulates specialized cellular responses to external stimuli. In Bipolaris maydis, a Chk1 MAPK orthologous to Fus3/Kss1 MAPKs of Saccharomyces cerevisiae is known to regulate various developmental processes, including the formation of appressoria. However, upstream factors that regulate the Chk1 cascade have not been well clarified. In this
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Cloning and functional characterization of gpd and α-tubulin promoters from Annulohypoxylon stygium, a companion fungus of Tremella fuciformis Mycoscience (IF 1.172) Pub Date : 2019-10-22 Dongmei Liu, Hanyu Zhu, Dwi Pujiana, Liesheng Zheng, Liguo Chen, Aimin Ma
Annulohypoxylon stygium is an ascomycete that helps Tremella fuciformis produce the fruiting body in wild state or artificial cultivation. Although the interaction between these two fungi is well known, the underlying molecular mechanism is limited. In this study, the 981 bp and 886 bp promoter sequences of glyceraldehyde-3-phosphate dehydrogenase (gpd) gene and α-tubulin gene have been cloned, respectively
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Effects of bacterial strains isolated from the ectomycorrhizal roots of Laccaria parva on sporocarp production by the fungus in vitro Mycoscience (IF 1.172) Pub Date : 2019-10-19 Keisuke Obase
The effects of bacteria isolated from ectomycorrhizas on sporocarp production of Laccaria parva were examined in vitro. Three bacterial strains of Bradyrhizobium were selected on the basis of their affinity for L. parva LL02 in previous confrontation tests: a positive or a negative strain that significantly increased or decreased hyphal extension areas, and a neutral strain that did not have significant
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First report of secondary metabolites, Violaceol I and Violaceol II produced by endophytic fungus, Trichoderma polyalthiae and their antimicrobial activity Mycoscience (IF 1.172) Pub Date : 2019-10-13 Katesaraporn Nuankeaw, Boonyanoot Chaiyosang, Thanapat Suebrasri, Somdej Kanokmedhakul, Saisaamorn Lumyong, Sophon Boonlue
Bioactive compounds of endophytic fungus Trichoderma polyalthiae were extracted from culture broth media. The crude extracts showed strong antimicrobial activity against human pathogens. Biologically active compounds were isolated and purified by chromatographic methods. The structures of the pure compounds were elucidated by spectroscopic methods. They were identified as Violaceol I and Violaceol
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Association between myxomycetes and the decay stage of coarse woody debris in an evergreen broadleaf forest in warm temperate Japan Mycoscience (IF 1.172) Pub Date : 2019-09-25 Yuichi Harakon, Kazunari Takahashi
Myxomycetes inhabit coarse woody debris in varying stages of decay; however, their ecology in the dead wood of evergreen broadleaf trees is not well known. In this study, we examined the relationships between myxomycete species and the decay stage of wood from fallen trees in an evergreen broadleaf forest in Japan. Myxomycete species richness and abundance were calculated for eight stages of decay
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Metarhizium brachyspermum sp. nov. (Clavicipitaceae), a new species parasitic on Elateridae from Japan Mycoscience (IF 1.172) Pub Date : 2019-09-07 Kohei Yamamoto, Muneyuki Ohmae, Takamichi Orihara
Ascomata of a Metarhizium species were collected from Tochigi Prefecture, Japan. The ascomata were similar to M. kalasinense in its host preference for elaterids, olive-green clavate stromata, and obliquely immersed perithecia, but the asci and ascospores were significantly shorter in length than those of the latter species. Furthermore, phylogenetic analyses based on the internal transcribed spacer
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Ectomycorrhiza synthesis and basidiome formation of an orchid symbiont of the genus Thelephora on Quercus serrata Mycoscience (IF 1.172) Pub Date : 2019-08-17 Takahiro Yagame, Nitaro Maekawa
We induced basidioma formation of a fungus belonging to the Thelephoraceae that was isolated from the orchid Cephalanthera falcata. The mycobiont was isolated from root, cultured on modified Melin-Norkrans medium, and then inoculated onto fine roots of a Quercus serrata (Fagaceae) seedling. After observation of ectomycorrhiza formation, the Q. serrata seedling was grown in a pot. Thirty-six mo after
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The effect of surface sterilization and the type of sterilizer on the genus composition of lichen-inhabiting fungi with notes on some frequently isolated genera Mycoscience (IF 1.172) Pub Date : 2019-07-30 Hiroshi Masumoto, Yousuke Degawa
Surface sterilization is generally used for isolating lichen-inhabiting fungi as well as endophytic fungi, and ethanol and sodium hypochlorite are commonly used as the sterilizer. However, there are few studies on whether the type of chemicals used for surface sterilization affects the isolation results of lichen-inhabiting fungi. In this study, the genus composition of the lichen-inhabiting fungi
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Aspergillus takadae, a novel heterothallic species of Aspergillus section Fumigati isolated from soil in China Mycoscience (IF 1.172) Pub Date : 2019-07-26 Tetsuhiro Matsuzawa, Paride Abliz, Takashi Yaguchi, Tohru Gonoi, Yoshikazu Horie
Aspergillus takadae is characterized by its heterothallic reproduction, pale yellow cleistothecia, broadly lenticular ascospores with two short equatorial crests and smooth convex surfaces, and broadly ellipsoidal to ovate conidia with a smooth wall. The validation of these novel species is supported further by the analyses of the β-tubulin, calmodulin, actin, and RPB2 sequences. In addition, the phylogenetic
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Taxonomic reconsideration of Tricholoma foliicola (Agaricales, Basidiomycota) based on basidiomata morphology, living culture characteristics, and phylogenetic analyses Mycoscience (IF 1.172) Pub Date : 2019-07-24 Naoki Endo, Shuji Ushijima, Eiji Nagasawa, Ryo Sugawara, Yudai Okuda, Kozue Sotome, Akira Nakagiri, Nitaro Maekawa
Tricholoma foliicola was taxonomically reevaluated based on analyses of the holotype and newly collected materials. Basidiospores of T. foliicola were irregularly ellipsoid, showing a cyanophilic reaction with or without tubercles. Phylogenetic analyses of the internal transcribed spacer (ITS) and large subunit 28S regions of the fungal nuclear ribosomal RNA gene demonstrated that T. foliicola is a
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Deconica cokeriana (Agaricales, Strophariaceae), a new combination Mycoscience (IF 1.172) Pub Date : 2019-07-10 Virginia Ramírez-Cruz, Alonso Cortés-Pérez, Jan Borovička, Alma Rosa Villalobos-Arámbula, Patrick B. Matheny, Laura Guzmán-Dávalos
Deconica cokeriana is proposed as a new combination based on morphological and molecular data. This species is fully described, discussed, and illustrated. Until now, D. cokeriana is only known from the eastern USA, specifically from Connecticut, Georgia, Ohio, and Tennessee.
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Differential expression of two gpd genes in the cultivated mushroom Pleurotus eryngii using RNA sequencing analysis Mycoscience (IF 1.172) Pub Date : 2019-06-25 Junjun Shang, Ruiheng Yang, Lihua Tang, Yán Li, Yan Li, Wenjun Mao, Ming Gong, Ying Wang, Yoichi Honda, Dapeng Bao
Transcriptional analysis showed that Pegpd1 was constitutively and highly expressed in various developmental stages of P. eryngii. The expression level of Pegpd2 was much lower than Pegpd1. The promoter of Pegpd1 contained the conserved cis-regulatory elements and could be used to drive heterologous gene expression in genetic transformation of P. eryngii.
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Mortierella oedorhiza, a new species forming a dichotomously branched rhizoid at the sporangiophore base Mycoscience (IF 1.172) Pub Date : 2019-06-24 Yusuke Takashima, Yousuke Degawa, Kazuhiko Narisawa
A new species, Mortierella oedorhiza, is described as a fungus forming a dichotomously branched rhizoid with a bulbil-like mass of hyphae at the base of the sporangiophore. The species is morphologically and phylogenetically similar to M. rostafinskii, which has rarely been reported. A trace of a columella on dehiscence of the sporangium and a dichotomously branched rhizoid are distinguishing characteristics
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Formvar membrane laid on artificial medium induces haustorium-like structure formation in powdery mildew fungi Mycoscience (IF 1.172) Pub Date : 2019-06-21 Naoto Yamaoka, Eiji Tanaka, Tsubasa Ogasahara, Honoka Tani, Kappei Kobayashi, Takashi Yaeno
We aimed to develop an artificial membrane system to observe the infection process of the obligate biotrophic powdery mildew fungi without the use of living plant cells. The conidia of Blumeria graminis and Erysiphe pisi conidia were inoculated on a formvar membrane laid on an artificial medium. Germinated conidia frequently formed appressoria and then penetrated the membrane to form haustorium-like
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Metarhizium aciculare sp. nov. for euvesperins A and B producing Metarhizium strains Mycoscience (IF 1.172) Pub Date : 2019-06-16 Hiromu Iwasaki, Toshiyuki Tokiwa, Mayu Shiina, Yukihiro Asami, Kazuro Shiomi, Satoshi Ōmura, Kenichi Nonaka
We have already found some Metarhizium isolates produce euvesperins A and B, which were circumventors of arbekacin resistance in MRSA. These isolates were obtained from soil samples collected from the Izu islands and Kannami town, Sizuoka, Japan. Using a combination of micro-morphological characteristics and multigene (SSU, LSU, TEF) phylogenics, the isolates were identified as a previously underscribed
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Assessment of genetic relatedness among commercial and wild strains of Agaricus bisporus using repetitive extragenic palindromic sequences and polymerase chain reaction Mycoscience (IF 1.172) Pub Date : 2019-06-12 Nader Rokni, Seyed Ali Akbar Sadati, Naser Safaie, Mohammad Ali Ebrahimi, Mohammad Samimifar
The genetic relatedness among 18 strains of Agaricus bisporus was assessed based on the fragment pattern analysis obtained by the amplification of genomic DNA by BOX, ERIC (ERICIR-I/ERIC2) and REP (REP1RI/REP2I) gene sequences. Based on the banding patterns of PCR-amplified products, eight putative groups among the 18 commercial and wild strains were recognized. REP-PCR generated multiple distinct