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Zinc metalloprotease ProA of Legionella pneumophila increases alveolar septal thickness in human lung tissue explants by collagen IV degradation Cell. Microbiol. (IF 3.43) Pub Date : 2021-01-24 Lina Scheithauer; Stefanie Thiem; Stefan Schmelz; Ansgar Dellmann; Konrad Büssow; René M.H.J. Brouwer; Can M. Ünal; Wulf Blankenfeldt; Michael Steinert
ProA is a secreted zinc metalloprotease of Legionella pneumophila causing lung damage in animal models of Legionnaires' disease. Here we demonstrate that ProA promotes infection of human lung tissue explants (HLTEs) and dissect the contribution to cell type specific replication and extracellular virulence mechanisms. For the first time, we reveal that co‐incubation of HLTEs with purified ProA causes
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Mycobacterium tuberculosis Glyceraldehyde‐3‐phosphate dehydrogenase plays a dual role‐as an adhesin and as a receptor for Plasmin(ogen) Cell. Microbiol. (IF 3.43) Pub Date : 2021-01-24 Zahid Gani; Vishant Mahendra Boradia; Ajay Kumar; Anil Patidar; Sharmila Talukdar; Eira Choudhary; Ranvir Singh; Nisheeth Agarwal; Manoj Raje; Chaaya Iyengar Raje
The spread of infection is directly determined by the ability of a pathogen to invade and infect host tissues. The process involves adherence due to host‐pathogen interactions and traversal into deeper tissues. Mycobacterium tuberculosis (Mtb) primarily infects the lung but is unique in its ability to infect almost any other organ of the human host including immune privileged sites such as the central
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Roles of Porphyromonas gulae proteases in bacterial and host cell biology Cell. Microbiol. (IF 3.43) Pub Date : 2021-01-24 Alam Saki Urmi; Hiroaki Inaba; Ryota Nomura; Sho Yoshida; Naoya Ohara; Fumitoshi Asai; Kazuhiko Nakano; Michiyo Matsumoto‐Nakano
Porphyromonas gulae, an animal‐derived periodontal pathogen, expresses several virulence factors, including fimbria, lipopolysaccharide (LPS), and proteases. We previously reported that its invasive efficiency was dependent on fimbriae types. In addition, P. gulae LPS increased inflammatory responses via toll‐like receptors. The present study was conducted to investigate the involvement of P. gulae
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Activation mechanisms of inflammasomes by bacterial toxins Cell. Microbiol. (IF 3.43) Pub Date : 2021-01-11 Weidong Jing; Jordan Lo Pilato; Callum Kay; Si Ming Man
Inflammasomes are cytosolic innate immune complexes, which assemble in mammalian cells in response to microbial components and endogenous danger signals. A major family of inflammasome activators is bacterial toxins. Inflammasome sensor proteins, such as the nucleotide‐binding oligomerisation domain‐like receptor (NLR) family members NLRP1b and NLRP3, and the tripartite motif family member Pyrin+ efflux
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Cover Image: A novel tick protein supports integrity of gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens (Cellular Microbiology 02/2021) Cell. Microbiol. (IF 3.43) Pub Date : 2021-01-19 Xiuli Yang; Juraj Koči; Alexis A. Smith; Xuran Zhuang; Kavita Sharma; Shraboni Dutta; Vipin S. Rana; Chrysoula Kitsou; Ozlem B. Yas; Emmanuel F. Mongodin; Utpal Pal
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Issue Information Cell. Microbiol. (IF 3.43) Pub Date : 2021-01-19
No abstract is available for this article.
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PfGBP2 is a novel G‐quadruplex binding protein in Plasmodium falciparum Cell. Microbiol. (IF 3.43) Pub Date : 2020-12-19 Pratima Gurung; Ana Rita Gomes; Rafael M. Martins; Stefan A. Juranek; Patrizia Alberti; Diane‐Ethna Mbang‐Benet; Serge Urbach; Elodie Gazanion; Vincent Guitard; Katrin Paeschke; Jose‐Juan Lopez‐Rubio
Guanine‐quadruplexes (G4s) are non‐canonical DNA structures that can regulate key biological processes such as transcription, replication and telomere maintenance in several organisms including eukaryotes, prokaryotes and viruses. Recent reports have identified the presence of G4s within the AT‐rich genome of Plasmodium falciparum, the protozoan parasite causing malaria. In Plasmodium, potential G4‐forming
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Very long O‐antigen chains of Salmonella Paratyphi A inhibit inflammasome activation and pyroptotic cell death Cell. Microbiol. (IF 3.43) Pub Date : 2020-12-23 Elli Mylona; Julia Sanchez‐Garrido; Trang Nguyen Hoang Thu; Sabina Dongol; Abhilasha Karkey; Stephen Baker; Avinash R. Shenoy; Gad Frankel
Salmonella Paratyphi A (SPtA) remains one of the leading causes of enteric (typhoid) fever. Yet, despite the recent increased rate of isolation from patients in Asia, our understanding of its pathogenesis is incomplete. Here we investigated inflammasome activation in human macrophages infected with SPtA. We found that SPtA induces GSDMD‐mediated pyroptosis via activation of caspase‐1, caspase‐4 and
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Valosin‐containing protein ATPase activity regulates the morphogenesis of Zika virus replication organelles and virus‐induced cell death Cell. Microbiol. (IF 3.43) Pub Date : 2021-01-11 Anaïs Anton; Clément Mazeaud; Wesley Freppel; Claudia Gilbert; Nicolas Tremblay; Aïssatou Aïcha Sow; Marie Roy; Ian Gaël Rodrigue‐Gervais; Laurent Chatel‐Chaix
With no available therapies, infections with Zika virus (ZIKV) constitute a major public health concern as they can lead to congenital microcephaly. In order to generate an intracellular environment favourable to viral replication, ZIKV induces endomembrane remodelling and the morphogenesis of replication factories via enigmatic mechanisms. In this study, we identified the AAA+ type ATPase valosin‐containing
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The protein kinase Ire1 impacts pathogenicity of Candida albicans by regulating homeostatic adaptation to endoplasmic reticulum stress Cell. Microbiol. (IF 3.43) Pub Date : 2021-01-05 Shabnam Sircaik; Elvira Román; Priyanka Bapat; Keunsook K. Lee; David R. Andes; Neil A.R. Gow; Clarissa J. Nobile; Jesús Pla; Sneh Lata Panwar
The unfolded protein response (UPR), crucial for the maintenance of ER homeostasis, is tied to the regulation of multiple cellular processes in pathogenic fungi. Here we show that C. albicans relies on an ER‐resident protein, inositol‐requiring enzyme 1 (Ire1) for sensing ER stress and activating the UPR. Compromised Ire1 function impacts cellular processes that are dependent on functional secretory
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The infectious propagules of Aspergillus fumigatus are coated with antimicrobial peptides Cell. Microbiol. (IF 3.43) Pub Date : 2020-12-17 Michaela Dümig; Jasmin Binder; Anastasia Gaculenko; Franziska Daul; Lex Winandy; Mike Hasenberg; Matthias Gunzer; Reinhard Fischer; Markus Künzler; Sven Krappmann
Fungal spores are unique cells that mediate dispersal and survival in the environment. For pathogenic fungi encountering a susceptible host, these specialised structures may serve as infectious particles. The main causative agent of the opportunistic disease aspergillosis, Aspergillus fumigatus, produces asexual spores, the conidia, that become dissipated by air flows or water currents but also serve
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Corrigendum Cell. Microbiol. (IF 3.43) Pub Date : 2021-01-01
In the published article of Rai et al. (2020), the authors found a mistake in the caption of Figure 4, panel E and would like to change the caption of Figure 4, panel E from “Hours post infection” to “Days of post infection”.
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Pathogen evasion strategies Cell. Microbiol. (IF 3.43) Pub Date : 2020-12-27 Benjamin B. A. Raymond
Pathogens are faced with a number of physical barriers upon entry in their animal host, whether it be the mucocilliary escalator of the respiratory tract or the acidic environment of the stomach. Following this, they must also overcome innate immune cells expressing pattern recognition receptors that are specialised in identifying and eliminating invading pathogens. During the course of co‐evolution
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The Coxiella burnetii effector protein CaeB modulates ER stress signaling and is required for efficient replication in Galleria mellonella Cell. Microbiol. (IF 3.43) Pub Date : 2020-12-23 Anja Friedrich; Paul A. Beare; Jan Schulze‐Luehrmann; Arne Cordsmeier; Tobias Pazen; Sophia Sonnewald; Anja Lührmann
The obligate intracellular pathogen C. burnetii is the causative agent of the zoonosis Q fever. C. burnetii infection can have severe outcomes due to the development of chronic infection. To establish and maintain an infection, C. burnetii depends on a functional type IVB secretion system (T4BSS) and, thus, on the translocation of effector proteins into the host cell. Here, we showed that the C. burnetii
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Cryptosporidium parvum infection induces autophagy in intestinal epithelial cells Cell. Microbiol. (IF 3.43) Pub Date : 2020-11-25 Shubha Priyamvada; Dulari Jayawardena; Jeet Bhalala; Anoop Kumar; Arivarasu N. Anbazhagan; Waddah A. Alrefai; Alip Borthakur; Pradeep K. Dudeja
Autophagy, a process of degradation and recycling of macromolecules and organelles to maintain cellular homeostasis, has also been shown to help eliminate invading pathogens. Conversely, various pathogens including parasites have been shown to modulate/exploit host autophagy facilitating their intracellular infectious cycle. In this regard, Cryptosporidium parvum (CP), a protozoan parasite of small
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Fluorescent protein‐based reporters reveal stress response of intracellular Salmonella enterica at level of single bacterial cells Cell. Microbiol. (IF 3.43) Pub Date : 2020-11-22 Marc Schulte; Katharina Olschewski; Michael Hensel
Intracellular bacteria such as Salmonella enterica are confronted with a broad array of defence mechanisms of their mammalian host cells. The ability to sense host cell‐imposed damages, and to mount efficient stress responses are crucial for survival and proliferation of intracellular pathogens. The various combinations of host defence mechanisms acting on intracellular bacteria and their individual
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Importance of two PDZ domains for the proteolytic and chaperone activities of Helicobacter pylori serine protease HtrA Cell. Microbiol. (IF 3.43) Pub Date : 2020-12-05 Urszula Zarzecka; Dorota Matkowska; Steffen Backert; Joanna Skorko‐Glonek
The Helicobacter pylori HtrA protein (HtrAHp) is an important virulence factor involved in the infection process by proteolysis of components of the tight (claudin‐8 and occludin) and adherens junctions (E‐cadherin) between epithelial cells. As a protease and chaperone, HtrAHp is involved in protein quality control, which is particularly important under stress conditions. HtrAHp contains a protease
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Als3‐mediated attachment of enolase on the surface of Candida albicans cells regulates their interactions with host proteins Cell. Microbiol. (IF 3.43) Pub Date : 2020-11-25 Justyna Karkowska‐Kuleta; Ewelina Wronowska; Dorota Satala; Marcin Zawrotniak; Grazyna Bras; Andrzej Kozik; Angela H. Nobbs; Maria Rapala‐Kozik
The multifunctional protein enolase has repeatedly been identified on the surface of numerous cell types, including a variety of pathogenic microorganisms. In Candida albicans—one of the most common fungal pathogens in humans—a surface‐exposed enolase form has been previously demonstrated to play an important role in candidal pathogenicity. In our current study, the presence of enolase at the fungal
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A conserved malaria parasite antigen Pb22 plays a critical role in male gametogenesis in Plasmodium berghei Cell. Microbiol. (IF 3.43) Pub Date : 2020-11-22 Fei Liu; Fan Yang; Yaru Wang; Minsheng Hong; Wenqi Zheng; Hui Min; Danni Li; Ying Jin; Takafumi Tsuboi; Liwang Cui; Yaming Cao
Gametogenesis, the formation of gametes from gametocytes, an essential step for malaria parasite transmission, is targeted by transmission‐blocking drugs and vaccines. We identified a conserved protein (PBANKA_0305900) in Plasmodium berghei, which encodes a protein of 22 kDa (thus named Pb22) and is expressed in both asexual stages and gametocytes. Its homologues are present in all Plasmodium species
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Cyclophilin 19 secreted in the host cell cytosol by Trypanosoma cruzi promotes ROS production required for parasite growth Cell. Microbiol. (IF 3.43) Pub Date : 2020-11-22 Gregory Pedroso dos Santos; Fernanda Midori Abukawa; Normanda Souza‐Melo; Laura Maria Alcântara; Paula Bittencourt‐Cunha; Carolina Borsoi Moraes; Bijay Kumar Jha; Bradford S. McGwire; Nilmar Silvio Moretti; Sergio Schenkman
Infection by Trypanosoma cruzi, the protozoan parasite that causes Chagas disease, depends on reactive oxygen species (ROS), which has been described to induce parasite proliferation in mammalian host cells. It is unknown how the parasite manages to increase host ROS levels. Here, we found that intracellular T. cruzi forms release in the host cytosol its major cyclophilin of 19 kDa (TcCyp19). Parasites
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Internal daughter formation of Toxoplasma gondii tachyzoites is coordinated by transcription factor TgAP2IX‐5 Cell. Microbiol. (IF 3.43) Pub Date : 2020-11-20 Chaoyue Wang; Dandan Hu; Xinming Tang; Xingju Song; Si Wang; Sixin Zhang; Chunhui Duan; Pei Sun; Jingxia Suo; Huiming Ma; Xun Suo; Xianyong Liu
Toxoplasma gondii rapidly propagates through endodyogeny of tachyzoites, a process in which daughter parasites divide within the cell of the mother parasite. Recent studies have revealed that transcription factors with AP2‐domain participate in the process of cell division in T. gondii. However, the concise regulation of the division cycles by AP2 proteins is poorly understood. In this study, we evaluated
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Cover Image: Plasmodium berghei sporozoites in nonreplicative vacuole are eliminated by a PI3P‐mediated autophagy‐independent pathway (Cellular Microbiology 1/2021) Cell. Microbiol. (IF 3.43) Pub Date : 2020-12-07 Annina Bindschedler; Rahel Wacker; Jessica Egli; Nina Eickel; Jacqueline Schmuckli‐Maurer; Blandine M. Franke‐Fayard; Chris J. Janse; Volker T. Heussler
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Issue Information Cell. Microbiol. (IF 3.43) Pub Date : 2020-12-07
No abstract is available for this article.
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Corrigendum Cell. Microbiol. (IF 3.43) Pub Date : 2020-11-09
In “Borrelia burgdorferi outer surface protein C (OspC) binds complement component C4b and confers bloodstream survival”, by Caine et al. (2017)which was published in volume 19, issue 12, September 2017, the authors wish to notify readers that we have not been able to reliably reproduce the data in Figure 5. The corresponding text “To determine if OspC is able to directly inhibit the complement cascade
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Molecular approaches to Malaria 2020 Cell. Microbiol. (IF 3.43) Pub Date : 2020-11-16 Tania F. de Koning‐Ward; Justin A. Boddey; Freya J.I. Fowkes
Twenty years ago the Molecular Approaches to Malaria conference was conceived as a forum to present the very latest advances in malaria research and to consolidate and forge new collaborative links between international researchers. The 6th MAM conference, held in February 2020 in Australia, provided 5 days of stimulating scientific exchange and highlighted the incredible malaria research conducted
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PE_PGRS: Vital proteins in promoting mycobacterial survival and modulating host immunity and metabolism Cell. Microbiol. (IF 3.43) Pub Date : 2020-11-20 Yan Xie; Yidan Zhou; Sheng Liu; Xiao‐Lian Zhang
Tuberculosis (TB), caused by Mycobacterium tuberculosis (M. tb), is the leading infectious cause of mortality worldwide. One of the key reasons for M. tb pathogenesis is the capability of M. tb to evade immune elimination and survive in macrophage, eventually causing chronic infection. However the pathogenicity mechanism of M. tb is not unclear yet, and thus diagnosis and therapy for TB remains a challenge
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Depletion of the mini‐chromosome maintenance complex binding protein allows the progression of cytokinesis despite abnormal karyokinesis during the asexual development of Plasmodium falciparum Cell. Microbiol. (IF 3.43) Pub Date : 2020-10-30 Sabrina Absalon; Jeffrey D. Dvorin
The eukaryotic cell cycle is typically divided into distinct phases with cytokinesis immediately following mitosis. To ensure proper cell division, each phase is tightly coordinated via feedback controls named checkpoints. During its asexual replication cycle, the malaria parasite Plasmodium falciparum undergoes multiple asynchronous rounds of mitosis with segregation of uncondensed chromosomes followed
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Cell microbiology interview Cell. Microbiol. (IF 3.43) Pub Date : 2020-11-04 Priscille Brodin
1 INTRODUCTION Priscille did her PhD at the Institut Gustave Roussy on the pharmacology of HIV integrase and then continued her Post‐Doctoral research at the Institut Pasteur, Paris, France with Professor Stewart T. Cole. After becoming a fellow from INSERM, Priscille moved to the Institut Pasteur Korea, Seoul in 2006 and started her team with the support of the Avenir Program. In 2011, Priscille was
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Toxoplasma gondii excreted/secreted proteases disrupt intercellular junction proteins in epithelial cell monolayers to facilitate tachyzoites paracellular migration Cell. Microbiol. (IF 3.43) Pub Date : 2020-10-27 Carlos J. Ramírez‐Flores; Rosalba Cruz‐Mirón; Noé Lagunas‐Cortés; Mónica Mondragón‐Castelán; Ricardo Mondragon‐Gonzalez; Sirenia González‐Pozos; Ricardo Mondragón‐Flores
Toxoplasma gondii shows high dissemination and migration properties across biological barriers infecting immunologically privileged organs. Toxoplasma uses different routes for dissemination; however, the mechanisms are not fully understood. Herein, we studied the effects of proteases present in excretion/secretion products (ESPs) of Toxoplasma on MDCK cell monolayers. Ultrastructural analysis showed
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Cover Image: Novel tumour suppressor roles for GZMA and RASGRP1 in Theileria annulata‐transformed macrophages and human B lymphoma cells (Cellular Microbiology 12/2020) Cell. Microbiol. (IF 3.43) Pub Date : 2020-11-05 Zineb Rchiad; Malak Haidar; Hifzur Rahman Ansari; Shahin Tajeri; Sara Mfarrej; Fathia Ben Rached; Abhinav Kaushik; Gordon Langsley; Arnab Pain
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Issue Information Cell. Microbiol. (IF 3.43) Pub Date : 2020-11-05
No abstract is available for this article.
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The roles of tetraspanins in bacterial infections. Cell. Microbiol. (IF 3.43) Pub Date : 2020-09-09 Jona Karam,Stéphane Méresse,Laurent Kremer,Wassim Daher
Tetraspanins, a wide family composed of 33 transmembrane proteins, are associated with different types of proteins through which they arbitrate important cellular processes such as fusion, adhesion, invasion, tissue differentiation and immunological responses. Tetraspanins share a comparable structural design, which consists of four hydrophobic transmembrane domains with cytoplasmic and extracellular
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Trained immunity and host-pathogen interactions. Cell. Microbiol. (IF 3.43) Pub Date : 2020-09-09 Adeline Peignier,Dane Parker
Infectious diseases are a leading cause of death worldwide with over 8 million fatalities accounted for in 2016. Solicitation of host immune defenses by vaccination is the treatment of choice to prevent these infections. It has long been thought that vaccine immunity was solely mediated by the adaptive immune system. However, over the past decade, numerous studies have shown that innate immune cells
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Macrobrachium rosenbergii nodavirus virus-like particles attach to fucosylated glycans in the gills of the giant freshwater prawn. Cell. Microbiol. (IF 3.43) Pub Date : 2020-08-30 Monsicha Somrit,Shin-Yi Yu,Jacques Le Pendu,Adrien Breiman,Yann Guérardel,Wattana Weerachatyanukul,Atthaboon Watthammawut
The Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white‐tail disease (WTD) in many species of shrimp and prawn, has been shown to infect hemocytes and tissues such as the gills and muscles. However, little is known about the host surface molecules to which MrNV attach to initiate infection. Therefore, the present study investigated the role of glycans as binding molecules for virus
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Corrigendum Cell. Microbiol. (IF 3.43) Pub Date : 2020-10-23
In “Fungal biotin homeostasis is essential for immune evasion after macrophage phagocytosis and virulence”, which was published in Volume 22, Issue 7 July 2020 e13197 (Sprenger et al., 2020), the authors would like to note that Figure 6a was incorrectly labelled. The amended version of Figure 6A can be found below.
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Corrigendum Cell. Microbiol. (IF 3.43) Pub Date : 2020-10-19
The authors of the article by Hu et al. (2013) would like to report a mistake in Figure 6A. In the published version of Figure 6A, the panels of the “p53‐pho(S15)” and “p53‐pho(S46)” groups in THP‐1 cells were inadvertently shown with incorrect images. The amended version of Figure 6A can be found below. The error does not affect the results or conclusions of the article. The authors would like to
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Using genetically encoded heme sensors to probe the mechanisms of heme uptake and homeostasis in Candida albicans Cell. Microbiol. (IF 3.43) Pub Date : 2020-10-26 Ziva Weissman; Mariel Pinsky; Rebecca K. Donegan; Amit R. Reddi; Daniel Kornitzer
Candida albicans is a major fungal pathogen that can utilise hemin and haemoglobin as iron sources in the iron‐scarce host environment. While C. albicans is a heme prototroph, we show here that it can also efficiently utilise external heme as a cellular heme source. Using genetically encoded ratiometric fluorescent heme sensors, we show that heme extracted from haemoglobin and free hemin enter the
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MicroRNA‐206 inhibits influenza A virus replication by targeting tankyrase 2 Cell. Microbiol. (IF 3.43) Pub Date : 2020-10-25 Gayan Bamunuarachchi; Xiaoyun Yang; Chaoqun Huang; Yurong Liang; Yujie Guo; Lin Liu
Due to the frequent mutations, influenza A virus (IAV) becomes resistant to anti‐viral drugs targeting influenza viral proteins. There are increasing interests in anti‐viral agents that target host cellular proteins required for virus replication. Tankyrase (TNKS) has poly (ADP‐ribose) polymerase activity and is a negative regulator of many host proteins. The objectives of this study are to study the
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Improved automatic detection of herpesvirus secondary envelopment stages in electron microscopy by augmenting training data with synthetic labelled images generated by a generative adversarial network Cell. Microbiol. (IF 3.43) Pub Date : 2020-10-18 Kavitha Shaga Devan; Paul Walther; Jens von Einem; Timo Ropinski; Hans A. Kestler; Clarissa Read
Detailed analysis of secondary envelopment of the herpesvirus human cytomegalovirus (HCMV) by transmission electron microscopy (TEM) is crucial for understanding the formation of infectious virions. Here, we present a convolutional neural network (CNN) that automatically recognises cytoplasmic capsids and distinguishes between three HCMV capsid envelopment stages in TEM images. 315 TEM images containing
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Plasmodium falciparum replication factor C subunit 1 is involved in genotoxic stress response Cell. Microbiol. (IF 3.43) Pub Date : 2020-10-11 Omar Sheriff; Aniweh Yaw; Soak Kuan Lai; hooi linn loo; Siu Kwan Sze; Peter Rainer Preiser
About half the world's population is at risk of malaria, with Plasmodium falciparum malaria being responsible for the most malaria related deaths globally. Antimalarial drugs such as chloroquine and artemisinin are directed towards the proliferating intra‐erythrocytic stages of the parasite, which is responsible for all the clinical symptoms of the disease. These antimalarial drugs have been reported
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Toxoplasma gondii GRA60 is an effector protein that modulates host cell autonomous immunity and contributes to virulence Cell. Microbiol. (IF 3.43) Pub Date : 2020-10-11 Mary Akinyi Nyonda; Pierre‐Mehdi Hammoudi; Shu Ye; Jessica Maire; Jean‐Baptiste Marq; Masahiro Yamamoto; Dominique Soldati‐Favre
Toxoplasma gondii infects virtually any nucleated cell and resides inside a non‐phagocytic vacuole surrounded by a parasitophorous vacuolar membrane (PVM). Pivotal to the restriction of T. gondii dissemination upon infection in murine cells is the recruitment of immunity regulated GTPases (IRGs) and guanylate binding proteins (GBPs) to the PVM that leads to pathogen elimination. The virulent T. gondii
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Co‐ and polymicrobial infections in the gut mucosa: The host–microbiota–pathogen perspective Cell. Microbiol. (IF 3.43) Pub Date : 2020-10-11 Teresa Frisan
Infections in humans occur in the context of complex niches where the pathogen interacts with both the host microenvironment and immune response, and the symbiotic microbial community. The polymicrobial nature of many human infections adds a further layer of complexity. The effect of co‐ or polymicrobial infections can result in enhanced severity due to pathogens cooperative interaction or reduced
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Viral entry and the ubiquitin‐proteasome system Cell. Microbiol. (IF 3.43) Pub Date : 2020-10-10 Seth M. Schneider; Becky H. Lee; Anthony V. Nicola
Viruses confiscate cellular components of the ubiquitin‐proteasome system (UPS) to facilitate many aspects of the infectious cycle. The 26S proteasome is an ATP‐dependent, multisubunit proteolytic machine present in all eukaryotic cells. The proteasome executes the controlled degradation of functional proteins, as well as the hydrolysis of aberrantly folded polypeptides. There is growing evidence for
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Aspergillus fumigatus Hsp90 interacts with the main components of the cell wall integrity pathway and cooperates in heat shock and cell wall stress adaptation Cell. Microbiol. (IF 3.43) Pub Date : 2020-10-03 Marina Campos Rocha; Karine Minari; João Henrique Tadini Marilhano Fabri; Joshua D. Kerkaert; Lisandra Marques Gava; Anderson Ferreira da Cunha; Robert A. Cramer; Júlio César Borges; Iran Malavazi
The initiation of Aspergillus fumigatus infection occurs via dormant conidia deposition into the airways. Therefore, conidial germination and subsequent hyphal extension and growth occur in a sustained heat shock (HS) environment promoted by the host. The cell wall integrity pathway (CWIP) and the essential eukaryotic chaperone Hsp90 are critical for fungi to survive HS. Although A. fumigatus is a
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Issue Information Cell. Microbiol. (IF 3.43) Pub Date : 2020-10-01
No abstract is available for this article.
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The ins and outs of host-microsporidia interactions during invasion, proliferation and exit. Cell. Microbiol. (IF 3.43) Pub Date : 2020-08-03 Hala Tamim El Jarkass,Aaron W Reinke
Microsporidia are a large group of fungal‐related obligate intracellular parasites. They are responsible for infections in humans as well as in agriculturally and environmentally important animals. Although microsporidia are abundant in nature, many of the molecular mechanisms employed during infection have remained enigmatic. In this review, we highlight recent work showing how microsporidia invade
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Extracellular vesicles released by anaerobic protozoan parasites: Current situation. Cell. Microbiol. (IF 3.43) Pub Date : 2020-08-28 Yesica Romina Nievas,Ayelen Lizarraga,Nehuen Salas,Verónica Mabel Cóceres,Natalia de Miguel
Extracellular vesicles (EVs) have emerged as a ubiquitous mechanism for transferring information between cells and organisms across all three kingdoms of life. Parasitic unicellular eukaryotes use EVs as vehicles for intercellular communication and host manipulation. Pathogenic protozoans are able to modulate the immune system of the host and establish infection by transferring a wide range of molecules
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Exosomes released from Shiga toxin 2a-treated human macrophages modulate inflammatory responses and induce cell death in toxin receptor expressing human cells. Cell. Microbiol. (IF 3.43) Pub Date : 2020-08-09 Kyung-Soo Lee,Jieun Lee,Pureum Lee,Chang-Ung Kim,Doo-Jin Kim,Yu-Jin Jeong,Young-Jun Park,Vernon L Tesh,Moo-Seung Lee
Shiga toxins (Stxs) produced by Stx‐producing Escherichia coli are the primarily virulence factors of hemolytic uremic syndrome and central nervous system (CNS) impairment. Although the precise mechanisms of toxin dissemination remain unclear, Stxs bind to extracellular vesicles (EVs). Exosomes, a subset of EVs, may play a key role in Stx‐mediated renal injury. To test this hypothesis, we isolated
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Inflammasome activation by Pseudomonas aeruginosa's ExlA pore-forming toxin is detrimental for the host. Cell. Microbiol. (IF 3.43) Pub Date : 2020-08-11 Stéphanie Bouillot,Stéphane Pont,Benoit Gallet,Christine Moriscot,Vincent Deruelle,Ina Attrée,Philippe Huber
During acute Pseudomonas aeruginosa infection, the inflammatory response is essential for bacterial clearance. Neutrophil recruitment can be initiated following the assembly of an inflammasome within sentinel macrophages, leading to activation of caspase‐1, which in turn triggers macrophage pyroptosis and IL‐1β/IL‐18 maturation. Inflammasome formation can be induced by a number of bacterial determinants
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The great host-pathogen war: U.K. Cellular microbiology meeting 2020. Cell. Microbiol. (IF 3.43) Pub Date : 2020-08-04 Jason S King,Daniel Humphreys,Jennifer L Rohn,Meera Unnikrishnan,Maximiliano G Gutierrez,Serge Mostowy
In 2019 we started a new annual meeting, aimed at bringing together researchers from across the United Kingdom studying cellular microbiology and the cell biology of host‐pathogen interactions. In contrast to large glamourous meetings, featuring the great and the good from across the world, we wanted to create a forum for early career researchers to present their work and enjoy lively discussion. In
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Expression of Concern Cell. Microbiol. (IF 3.43) Pub Date : 2020-09-22
“MiRNA‐218 regulates osteoclast differentiation and inflammation response in periodontitis rats through MMP9”, Cell. Microbiol. 2019;21:e12979, by Jie Guo, Xuemin Zeng, Jie Miao, Chunpeng Liu, Fulan Wei, Dongxu Liu, Zhong Zheng, Kang Ting, Chunling Wang, and Yi Liu.
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The CCCTC‐binding factor CTCF represses hepatitis B virus enhancer I and regulates viral transcription Cell. Microbiol. (IF 3.43) Pub Date : 2020-10-01 Valentina D'Arienzo; Jack Ferguson; Guillaume Giraud; Fleur Chapus; James M. Harris; Peter A. C. Wing; Adam Claydon; Sophia Begum; Xiaodong Zhuang; Peter Balfe; Barbara Testoni; Jane A. McKeating; Joanna L. Parish
Hepatitis B virus (HBV) infection is of global importance with over 2 billion people exposed to the virus during their lifetime and at risk of progressive liver disease, cirrhosis and hepatocellular carcinoma. HBV is a member of the Hepadnaviridae family that replicates via episomal copies of a covalently closed circular DNA (cccDNA) genome. The chromatinization of this small viral genome, with overlapping
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A novel tick protein supports integrity of gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens Cell. Microbiol. (IF 3.43) Pub Date : 2020-10-01 Xiuli Yang; Juraj Koči; Alexis A. Smith; Xuran Zhuang; Kavita Sharma; Shraboni Dutta; Vipin S. Rana; Chrysoula Kitsou; Ozlem B. Yas; Emmanuel F. Mongodin; Utpal Pal
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Surface area‐to‐volume ratio, not cellular viscoelasticity, is the major determinant of red blood cell traversal through small channels Cell. Microbiol. (IF 3.43) Pub Date : 2020-09-27 Arman Namvar; Adam J. Blanch; Matthew W. Dixon; Olivia M. S. Carmo; Boyin Liu; Snigdha Tiash; Oliver Looker; Dean Andrew; Li‐Jin Chan; Wai‐Hong Tham; Peter V. S. Lee; Vijay Rajagopal; Leann Tilley
The remarkable deformability of red blood cells (RBCs) depends on the viscoelasticity of the plasma membrane and cell contents and the surface area to volume (SA:V) ratio; however, it remains unclear which of these factors is the key determinant for passage through small capillaries. We used a microfluidic device to examine the traversal of normal, stiffened, swollen, parasitised and immature RBCs
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Plasmodium berghei sporozoites in nonreplicative vacuoles are eliminated by a PI3P-mediated autophagy-independent pathway. Cell. Microbiol. (IF 3.43) Pub Date : 2020-09-26 Annina Bindschedler,Rahel Wacker,Jessica Egli,Nina Eickel,Jacqueline Schmuckli-Maurer,Blandine M Franke-Fayard,Chris J Janse,Volker T Heussler
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Fungal factors involved in host immune evasion, modulation and exploitation during infection Cell. Microbiol. (IF 3.43) Pub Date : 2020-09-26 Annika König; Rita Müller; Selene Mogavero; Bernhard Hube
Human and plant pathogenic fungi have a major impact on public health and agriculture. Although these fungi infect very diverse hosts and are often highly adapted to specific host niches, they share surprisingly similar mechanisms that mediate immune evasion, modulation of distinct host targets and exploitation of host nutrients, highlighting that successful strategies have evolved independently among
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An apicoplast-resident folate transporter is essential for sporogony of malaria parasites. Cell. Microbiol. (IF 3.43) Pub Date : 2020-09-25 Francois Korbmacher,Benjamin Drepper,Theo Sanderson,Peer Martin,Thomas Stach,Alexander G Maier,Kai Matuschewski,Joachim M Matz
Malaria parasites are fast replicating unicellular organisms and require substantial amounts of folate for DNA synthesis. Despite the central role of this critical co‐factor for parasite survival, only little is known about intraparasitic folate trafficking in Plasmodium. Here, we report on the expression, subcellular localisation and function of the parasite's folate transporter 2 (FT2) during life
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Rab7D small GTPase is involved in phago‐, trogocytosis and cytoskeletal reorganization in the enteric protozoan Entamoeba histolytica Cell. Microbiol. (IF 3.43) Pub Date : 2020-09-25 Yumiko Saito‐Nakano; Ratna Wahyuni; Kumiko Nakada‐Tsukui; Kentaro Tomii; Tomoyoshi Nozaki
Rab small GTPases regulate membrane traffic between distinct cellular compartments of all eukaryotes in a tempo‐spatially specific fashion. Rab small GTPases are also involved in the regulation of cytoskeleton and signalling. Membrane traffic and cytoskeletal regulation play pivotal role in the pathogenesis of Entamoeba histolytica, which is a protozoan parasite responsible for human amebiasis. E.
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An alloy of zinc and innate immunity: Galvanising host defence against infection Cell. Microbiol. (IF 3.43) Pub Date : 2020-09-25 Jessica B. von Pein; Claudia J. Stocks; Mark A. Schembri; Ronan Kapetanovic; Matthew J. Sweet
Innate immune cells such as macrophages and neutrophils initiate protective inflammatory responses and engage antimicrobial responses to provide frontline defence against invading pathogens. These cells can both restrict the availability of certain transition metals that are essential for microbial growth and direct toxic concentrations of metals towards pathogens as antimicrobial responses. Zinc is
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Intestinal inflammation alters mucosal carbohydrate foraging and monosaccharide incorporation into microbial glycans Cell. Microbiol. (IF 3.43) Pub Date : 2020-09-25 Gisela Adrienne Weiss; Thomas Grabinger; Jesus Glaus Garzon; Tobias Hasler; Anna Greppi; Christophe Lacroix; Nikolay Khanzhin; Thierry Hennet
Endogenous carbohydrates released from the intestinal mucus represent a constant source of nutrients to the intestinal microbiota. Mucus‐derived carbohydrates can also be used as building blocks in the biosynthesis of bacterial cell wall components, thereby influencing host mucosal immunity. To assess the uptake of endogenous carbohydrates by gut microbes in healthy mice and during intestinal inflammation
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