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Antifungal activity of MAF-1A peptide against Candida albicans Int. Microbiol. (IF 1.833) Pub Date : 2021-01-16 Rong Cheng, Qiang Xu, Fangfang Hu, Hongling Li, Bin Yang, Zonggang Duan, Kai Zhang, Jianwei Wu, Wei Li, Zhenhua Luo
Invasive candidiasis is a major threat to human health, and Candida albicans is the most common pathogenic species responsible for this condition. The incidence of drug-resistant strains of C. albicans is rising, necessitating the development of new antifungal drugs. Antimicrobial peptides (AMPs) have recently attracted attention due to their unique ability to evade the drug resistance of microorganisms
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Differential response of photosynthetic apparatus towards alkaline pH treatment in NIES-39 and PCC 7345 strains of Arthrospira platensis Int. Microbiol. (IF 1.833) Pub Date : 2021-01-12 Monika Mahesh Jangir, Shibasish Chowdhury, Vani Bhagavatula
Alkaline stress is one of the severe abiotic stresses, which is not well studied so far, especially among cyanobacteria. To affirm the characteristics of alkaline stress and the subsequent adaptive responses in Arthrospira platensis NIES-39 and Arthrospira platensis PCC 7345, photosynthetic pigments, spectral properties of thylakoids, PSII and PSI activities, and pigment-protein profiles of thylakoids
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Diversity of rhizobial and non-rhizobial bacteria nodulating wild ancestors of grain legume crop plants Int. Microbiol. (IF 1.833) Pub Date : 2021-01-10 Sevil Basbuga, Selcuk Basbuga, Fatih Yayla, Ahmed M. Mahmoud, Canan Can
Chickpeas, lentils, and peas are the oldest grain legume species that spread to other regions after their first domestication in Fertile Crescent, and they could reveal the rhizobial evolution in relation to the microsymbionts of wild species in this region. This study investigated the phenotypic and genotypic diversity of the nodule-forming rhizobial bacteria recovered from Pisum sativum subsp., Cicer
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Bacterial community characterization of Batura Glacier in the Karakoram Range of Pakistan Int. Microbiol. (IF 1.833) Pub Date : 2021-01-06 Pervaiz Ali, Feng Chen, Fariha Hassan, Ana Sosa, Samiullah Khan, Malik Badshah, Aamer Ali Shah
High-altitude cold habitats of the Karakoram are rarely explored for their bacterial community characterization and metabolite productions. In the present study, bacterial communities in ice, water, and sediments of Batura Glacier were investigated using culture-dependent and culture-independent methods. Twenty-seven cold-adapted bacterial strains (mostly psychrotrophic) were isolated using R2A, Tryptic
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Aptamer-based approaches for the detection of waterborne pathogens Int. Microbiol. (IF 1.833) Pub Date : 2021-01-06 Archana Vishwakarma, Roshni Lal, Mohandass Ramya
Waterborne ailments pose a serious threat to public health and are a huge economic burden. Lack of hygiene in drinking and recreational water is the chief source of microbial pathogens in developing countries. Poor water quality and sanitation account for more than 3.4 million deaths a year worldwide. This has urged authorities and researchers to explore different avenues of pathogen detection. There
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Transcriptional analysis of the lichenase-like gene cel12A of the filamentous fungus Stachybotrys atra BP-A and its relevance for lignocellulose depolymerization Int. Microbiol. (IF 1.833) Pub Date : 2021-01-06 Pere Picart, F. I. Javier Pastor, Margarita Orejas
To rationally optimize the production of industrial enzymes by molecular means requires previous knowledge of the regulatory circuits controlling the expression of the corresponding genes. The genus Stachybotrys is an outstanding producer of cellulose-degrading enzymes. Previous studies isolated and characterized the lichenase-like/non-typical cellulase Cel12A of S. atra (AKA S. chartarum) belonging
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Purification, characterization, and anticancer and antioxidant activities of l -glutaminase from Aspergillus versicolor Faesay4 Int. Microbiol. (IF 1.833) Pub Date : 2021-01-02 Mohamed F. Awad, Fareed Shawky El-Shenawy, Mervat Morsy Abbas Ahmed El-Gendy, Esraa Ahmed Mohamed El-Bondkly
l-Glutaminase is an amidohydrolase which can act as a vital chemotherapeutic agent against various malignancies. In the present work, l-glutaminase productivity from Aspergillus versicolor Faesay4 was significantly increased by 7.72-fold (from 12.33 ± 0.47 to 95.15 ± 0.89 U/mL) by optimizing submerged fermentation parameters in Czapek’s Dox (CZD) medium including an incubation period from 3 (12.33 ± 0
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SARS-CoV-2: a new dimension to our understanding of coronaviruses Int. Microbiol. (IF 1.833) Pub Date : 2020-11-24 Yusha Araf, Nairita Ahsan Faruqui, Saeed Anwar, Mohammad Jakir Hosen
Coronaviruses have marked their significant emergence since the twenty-first century with the outbreaks of three out of the seven existing human coronaviruses, including the severe acute respiratory syndrome coronavirus (SARS-CoV) in 2003, Middle East respiratory syndrome coronavirus (MERS-CoV) in 2012, and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 2019. These viruses have not
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Distribution of Burkholderia cepacia complex species isolated from industrial processes and contaminated products in Argentina Int. Microbiol. (IF 1.833) Pub Date : 2020-11-12 A. López De Volder, S. Teves, A. Isasmendi, J. L. Pinheiro, L. Ibarra, N. Breglia, T. Herrera, M. Vazquez, C. Hernandez, José Degrossi
Burkholderia cepacia complex (Bcc) members have clinical relevance as opportunistic pathogens in patients with cystic fibrosis and are responsible of numerous nosocomial infections. These closely related bacteria are also reported as frequent contaminants of industrial products. In this retrospective study, we use PCR and recA gene sequence analysis to identify at species level Bcc isolates recovered
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Development of a SCAR marker linked to fungal pathogenicity of rice blast fungus Magnaporthe Oryzae Int. Microbiol. (IF 1.833) Pub Date : 2020-11-07 Nguyen Bao Quoc, Ho Thi Thu Trang, Nguyen Doan Nguyen Phuong, Nguyen Ngoc Bao Chau, Chatchawan Jantasuriyarat
PCR-based molecular approaches including RAPD (random amplified polymorphic DNA), ISSR (inter-simple sequence repeat), and SRAP (sequence-related amplified polymorphism) are commonly used to analyze genetic diversity. The aims of this study are to analyze genetic diversity of M. oryzae isolates using PCR-based molecular approaches such as RAPD, ISSR, and SRAP and to develop SCAR marker linked to the
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Detection of Trypanosoma cruzi DNA in false negative samples of collected triatomines, xenodiagnosis material, and biopsies of experimentally infected animals Int. Microbiol. (IF 1.833) Pub Date : 2020-11-06 Leidi Herrera, Cruz Manuel Aguilar, Antonio Morocoima, Mercedes Viettri, María Lares, Elizabeth Ferrer
Direct test over the gut material from triatomine vectors and xenodiagnosis over mammalian hosts are classical techniques for Trypanosoma cruzi parasitological diagnosis. Nevertheless, negative results can be a source of uncertainty. Experimental models have allowed evaluating the tissue invasion of different strains of T. cruzi, but conventional techniques for tissue biopsies involve time-consuming
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Immunodiagnostic of Vibrio cholerae O1 using localized surface plasmon resonance (LSPR) biosensor Int. Microbiol. (IF 1.833) Pub Date : 2020-11-04 Ghazale Faridfar, Mehdi Zeinoddini, Saeid Akbarzedehkolahi, Shahin Faridfar, Afshin Samimi Nemati
V. cholerae O1 is a gram-negative bacilli that causes an acute gastrointestinal disease called cholera. V. cholerae can enter into the biofilm phase in a period of life; hence, it is challenging to recognize these bacteria. Accordingly, using localized surface plasmon resonance (LSPR) features of the nanoparticles, an accurate detection method based on the antigen-antibody reaction was used. Ordinarily
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A newly isolated Bacillus amyloliquefaciens SRB04 for the synthesis of selenium nanoparticles with potential antibacterial properties Int. Microbiol. (IF 1.833) Pub Date : 2020-10-29 Morahem Ashengroph, Seyedeh-Roya Hosseini
The aim of this study was to isolate and characterize marine bacterial strains capable of converting selenite to elemental selenium with the formation of Se nanoparticles (SeNPs). For the first time, a novel marine strain belonging to Bacillus amyloliquefaciens (GenBank accession no. MK392020) was isolated from the coast of the Caspian Sea and characterized based on its ability for transformation of
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Azole derivatives with naphthalene showing potent antifungal effects against planktonic and biofilm forms of Candida spp.: an in vitro and in silico study. Int. Microbiol. (IF 1.833) Pub Date : 2020-09-05 Suat Sari,Ebru Koçak,Didem Kart,Zeynep Özdemir,M Fahir Acar,Burcu Sayoğlu,Arzu Karakurt,Sevim Dalkara
Candida infections pose a serious public health threat due to increasing drug resistance. Azoles are first-line antifungal drugs for fungal infections. In this study, we tested an in-house azole collection incorporating naphthalene ring to find hits against planktonic and biofilm forms of resistant Candida spp. In the collection, potent derivatives were identified against the susceptible strains of
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Comparative genome analysis of 12 Shigella sonnei strains: virulence, resistance, and their interactions. Int. Microbiol. (IF 1.833) Pub Date : 2020-09-03 Zuobin Zhu,Liang Wang,Huimin Qian,Feng Gu,Ying Li,Heng Zhang,Ying Chen,Jiajia Shi,Ping Ma,Changjun Bao,Bing Gu
Shigellosis is a highly infectious disease that is mainly transmitted via fecal-oral contact of the bacteria Shigella. Four species have been identified in Shigella genus, among which Shigella flexneri is used to be the most prevalent species globally and commonly isolated from developing countries. However, it is being replaced by Shigella sonnei that is currently the main causative agent for dysentery
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Detection of Mycobacterium kansasii using a combination of loop-mediated isothermal amplification (LAMP) and lateral flow biosensors. Int. Microbiol. (IF 1.833) Pub Date : 2020-09-02 Chuang Chen,Jia Lu,Bo Long,Zhengyuan Rao,Yuan Gao,Weina Wang,Wenfeng Gao,Jun Yang,Shu Zhang
Mycobacterium kansasii is an opportunistic pathogen that causes both intrapulmonary and extrapulmonary infections. The symptoms of the pulmonary diseases caused by M. kansasii closely resemble Mycobacterium tuberculosis. Rapid and accurate differentiation of M. kansasii from M. tuberculosis, as well as other mycobacteria, is crucial for developing effective therapeutics and disease treatment. In this
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RNA interference as a promising treatment against SARS-CoV-2. Int. Microbiol. (IF 1.833) Pub Date : 2020-09-01 Ahmed Donia,Habib Bokhari
Until now, there is no current vaccine or treatment against SARS-CoV-2. There are previous successful RNAi studies performed on SARS-CoV. Therefore, similar line of investigation against SARS-CoV-2 could be successful.
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A survey of intact low-density polyethylene film biodegradation by terrestrial Actinobacterial species. Int. Microbiol. (IF 1.833) Pub Date : 2020-08-22 Zahra Soleimani,Sara Gharavi,Mohammadreza Soudi,Zahra Moosavi-Nejad
Low-density polyethylene (LDPE) polymer is mainly used in the production of plastic bags and food packaging making up the largest volume of plastic pollutions. These polymers are potential substrates for bacteria in the bioremediation process. In this study, soil samples were collected from different plastic landfills in Iran and subsequently enriched in specific media (polyethylene as carbon source)
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Virulence and in vitro antifungal susceptibility of Candida albicans and Candida catenulata from laying hens. Int. Microbiol. (IF 1.833) Pub Date : 2020-08-09 Wafa Rhimi,Chioma Inyang Aneke,Giada Annoscia,Antonio Camarda,Adriana Mosca,Cinzia Cantacessi,Domenico Otranto,Claudia Cafarchia
In spite of evidence that domestic and wild birds may act as carriers of human pathogenic fungi, data on the role of laying hens as reservoirs of drug resistant and virulent yeasts is lacking. Here, we assess several virulence factors (phospholipase and haemolysin activity) and the antifungal susceptibility profiles of 84 Candida albicans and 17 Candida catenulata strains isolated from cloacae (group
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The endosphere microbial communities, a great promise in agriculture. Int. Microbiol. (IF 1.833) Pub Date : 2020-07-31 Bartholomew Saanu Adeleke,Olubukola Oluranti Babalola
Agricultural food production and sustainability need intensification to address the current global food supply to meet human demand. The continuous human population increase and other anthropogenic activities threaten food security. Agrochemical inputs have long been used in conventional agricultural systems to boost crop productivity, but they are disadvantageous to a safe environment. Towards developing
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Gut bacteria of Varanus salvator possess potential antitumour molecules. Int. Microbiol. (IF 1.833) Pub Date : 2020-07-31 Morhanavallee Soopramanien,Naveed Ahmed Khan,Kuppusamy Sagathevan,Ruqaiyyah Siddiqui
Pollution, unhygienic conditions and organic waste are detrimental to human health. On the contrary, animals living in polluted environments, feeding on organic waste and exposed to noxious agents such as heavy metals must possess remarkable properties against contracting diseases. Species such as cockroaches and water monitor lizards thrive in unhygienic conditions and feed on decaying matter. Here
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The principle of distance during COVID-19 outbreak in Korea. Int. Microbiol. (IF 1.833) Pub Date : 2020-07-27 Kyoo-Man Ha
The majority of confirmed COVID-19 cases in Korea have been related to mass infection. The nation must urgently promote the distance awareness approach during emergency response and discourage the distance unawareness approach. Other nations may also apply the principle of distance to their pandemic management.
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Penicillin G acylase production by Mucor griseocyanus and the partial genetic analysis of its pga gene. Int. Microbiol. (IF 1.833) Pub Date : 2020-07-23 Juan C Cano-Cabrera,Lissethe Palomo-Ligas,Adriana C Flores-Gallegos,José L Martínez-Hernández,Raúl Rodríguez-Herrera
Penicillin acylases (penicillin amidohydrolase, EC 3.5.1.11) are a group of enzymes with many applications within the pharmaceutical industry, and one of them is the production of semi-synthetic beta-lactam antibiotics. This enzyme is mainly produced by bacteria but also by some fungi. In the present study, the filamentous fungus Mucor griseocyanus was used to produce penicillin acylase enzyme (PGA)
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Transcriptome Analysis of Dimorphic Fungus Sporothrix schenckii Exposed to Temperature Stress. Int. Microbiol. (IF 1.833) Pub Date : 2020-07-20 Dan He,Xiaowei Zhang,Song Gao,Hailong You,Yanbing Zhao,Li Wang
Purpose Sporothrix schenckii is a thermally dimorphic fungus. In a saprotrophic environment or culturing at 25 °C, it grows as mycelia, whereas in host tissues or culturing at 37 °C, it undergoes dimorphic transition and division into pathogenic yeast cells. S. schenckii can cause serious disseminated sporotrichosis in immunocompromised hosts and presents an emerging global health problem. The mycelium-to-yeast
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Evaluation of different culture media for detection and quantification of H. pylori in environmental and clinical samples. Int. Microbiol. (IF 1.833) Pub Date : 2020-06-30 Irene Hortelano,Yolanda Moreno,Fidson Juarismi Vesga,María Antonia Ferrús
The objective of the present study was to establish the most suitable culture medium for the isolation of H. pylori from environmental and clinical samples. Ten different culture media were compared and evaluated. Four of them had been previously described and were modified in this study. The rest of the media were designed de novo. Three different matrices, tap water, wastewater, and feces, were inoculated
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Lead and cadmium-resistant bacterial species isolated from heavy metal-contaminated soils show plant growth-promoting traits. Int. Microbiol. (IF 1.833) Pub Date : 2020-06-13 Samaneh Abdollahi,Ahmad Golchin,Fatemeh Shahryari
Application of metal-resistant rhizobacteria is a promising approach for detoxification and bioremediation of contaminated soils. In order to isolate, identify, and characterize lead and cadmium-resistant bacteria, nearly 30 soil samples were collected from heavy metal-contaminated sites, and five resistant bacterial strains were isolated and identified based on their cultural, physiological, biochemical
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Occurrence of ESBL-producing Escherichia coli in soils subjected to livestock grazing in Azores archipelago: an environment-health pollution issue? Int. Microbiol. (IF 1.833) Pub Date : 2020-06-08 Vanessa Silva,Fernando Peixoto,Carolina Parelho,Patrícia Garcia,Armindo Rodrigues,Adriana Silva,Isabel Carvalho,José Eduardo Pereira,Gilberto Igrejas,Patrícia Alexandra Curado Quintas Dinis Poeta
Antibiotics are successful drugs used in human and animal therapy; however, they must be considered as environmental pollutants. This study aims to isolate and characterize the extended-spectrum β-lactamase (ESBL) producing Escherichia coli soil from Azores Archipelago subjected to livestock agricultural practices. Twenty-four soil samples were collected from three different pasture systems with different
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Phenotypic, stress tolerance, and plant growth promoting characteristics of rhizobial isolates of grass pea. Int. Microbiol. (IF 1.833) Pub Date : 2020-06-03 Mussa Adal Mohammed,Million Tadege Chernet,Fassil Assefa Tuji
Grass pea (Lathyrus sativus L.) is widely cultivated for food and feed in some developing countries including Ethiopia. However, due to its overexaggerated neuro-lathyrism alkaloid causing paralysis of limbs, it failed to attract attention of the research community and is one of the most neglected orphan crops in the world. But, the crop is considered an insurance crop by resource-poor farmers due
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Legionella pneumophila Attachment to Biofilms of an Acidovorax Isolate from a Drinking Water-Consortium Requires the Lcl-Adhesin Protein. Int. Microbiol. (IF 1.833) Pub Date : 2020-05-25 Christa H Chatfield,Jenna Zaia,Cassidy Sauer
Human infection by Legionella pneumophila (Lpn) only occurs via contaminated water from man-made sources, and eradication of these bacteria from man-made water systems is complicated by biofilm colonization. Using a continuously fed biofilm reactor model, we grew a biofilm consortium from potable water that was able to prolong recovery of Lpn CFU from biofilms. This effect was recreated using a subset
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Molecular and serological typing of Streptococcus mutans strains isolated from young Galician population: relationship with the oral health status. Int. Microbiol. (IF 1.833) Pub Date : 2020-05-22 Nancy Saltos Rosero,Rafael Seoane Prado,Antonio Aguilera Guirao,Ysabel Santos
The aim of this study was to determine the prevalence of Streptococcus mutans and its serotypes in samples from oral cavity of young Galician population and their relationship with the oral health state. The variables generally associated with dental caries, such as salivary flow rate, buffering capacity, eating habits, and lifestyle, were also analysed. No relationship was found between the variables
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Identification of the role of toxin B in the virulence of Clostridioides difficile based on integrated bioinformatics analyses. Int. Microbiol. (IF 1.833) Pub Date : 2020-05-09 Yan Gao,Weihu Gao,Jingwei Cheng,Liyan Ma,Jianrong Su
PURPOSE Clostridioides difficile toxin B (TcdB) plays a critical role in C. difficile infection (CDI), a common and costly healthcare-associated disease. The aim of the current study was to explore the intracellular and potent systemic effects of TcdB on human colon epithelial cells utilizing Gene Expression Omnibus and bioinformatic methods. METHODS Two datasets (GSE63880 and GSE29008) were collected
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Shifts in the gut microbiota of mice in response to dexamethasone administration. Int. Microbiol. (IF 1.833) Pub Date : 2020-05-01 Haichao Zhao,Xueyuan Jiang,Weihua Chu
Glucocorticoids (GCs) are an important anti-inflammatory drug, used widely, regardless of its side effects. GCs can affect intestinal flora directly or indirectly, though few studies have focused on the changes of gut microbiota composition. In this study, ICR mice were randomly divided into three groups, gavage administration with saline, and different doses of dexamethasone (DEX): 0.1 mg/kg and 1
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Comparative analysis of bacterial community and functional species in oil reservoirs with different in situ temperatures. Int. Microbiol. (IF 1.833) Pub Date : 2020-04-27 Yuexin Tian,Shuwen Xue,Yanling Ma
Temperature is supposed to be one of the primary drivers for the bacterial diversification as well as hydrocarbon formation process of oil reservoirs. However, the bacterial community compositions are not systematically elucidated in oil reservoirs with different temperatures. Herein, the diversity of indigenous bacteria and the functional species in the water samples from oil reservoirs with different
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The role of Pseudomonas aeruginosa DesB in pathogen-host interaction. Int. Microbiol. (IF 1.833) Pub Date : 2020-04-22 Jimyeong Ha,Sejeong Kim,Jeeyeon Lee,Heeyoung Lee,Yukyung Choi,Hyemin Oh,Yohan Yoon,Kyoung-Hee Choi
Pseudomonas aeruginosa, commonly found in environments, can cause chronic lung disease in immunocompromised patients. In previous study, an aerobic desaturase (DesB) in P. aeruginosa exerted considerable effects on virulence factor production. The objective of this study was to analyze the role of DesB on the virulence traits of P. aeruginosa in the host. For the in vitro experiments, cells and supernatants
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Antimicrobial activity of bacteriocins produced by Enterococcus isolates recovered from Egyptian homemade dairy products against some foodborne pathogens. Int. Microbiol. (IF 1.833) Pub Date : 2020-04-18 Fatma I Sonbol,Ahmed A Abdel Aziz,Tarek E El-Banna,Omnia M Al-Fakhrany
The increasing mandate for fresh-like food products and the possible hazards of chemically preserved foods necessitate the search for alternatives. Bacteriocins represent a promising food biopreservative. In the present study, one hundred enterococci isolates recovered from Egyptian raw cow milk and homemade dairy products were screened for bacteriocin production. The overall detection rate was 10%
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Pioneering women of microbiology in Spain. Int. Microbiol. (IF 1.833) Pub Date : 2020-04-15 Alfonso V Carrascosa Santiago
Presented herein are the trajectories of four women who can be considered pioneers of microbiology in Spain. Three of them have been studied before, but never presented as pioneers of microbiology, and their lives are briefly reviewed: Zoe Rosinach Pedrol, a pioneering microbiologist in the health care field; Isabel Torán del Carré, in the agri-food sector; and Luz Zalduegui Gabilondo in the veterinary
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Gut bacteria of animals living in polluted environments exhibit broad-spectrum antibacterial activities. Int. Microbiol. (IF 1.833) Pub Date : 2020-03-03 Noor Akbar,Ruqaiyyah Siddiqui,K Sagathevan,Naveed Ahmed Khan
Infectious diseases, in particular bacterial infections, are the leading cause of morbidity and mortality posing a global threat to human health. The emergence of antibiotic resistance has exacerbated the problem further. Hence, there is a need to search for novel sources of antibacterials. Herein, we explored gut bacteria of a variety of animals living in polluted environments for their antibacterial
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Effects of Trichoderma asperellum and its siderophores on endogenous auxin in Arabidopsis thaliana under iron-deficiency stress. Int. Microbiol. (IF 1.833) Pub Date : 2020-02-20 Lei Zhao,Yue Wang,Shuang Kong
Iron (Fe) deficiency is one of the major limiting factors affecting crop yields. Trichoderma asperellum Q1, a biocontrol and plant growth promoting fungus, can produce the siderophore which has a high affinity to Fe3+ in the absence of iron. In this study, Trichoderma asperellum Q1 was found to be able to promote growth of Arabidopsis thaliana in an iron-deficient or insoluble iron-containing (Fe2O3)
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Implications of bacteriophages on the acquisition and spread of antibiotic resistance in the environment. Int. Microbiol. (IF 1.833) Pub Date : 2020-01-30 José Luis Balcázar
Although bacteriophages (or simply phages) are the most abundant biological entities and have the potential to transfer genetic material between bacterial hosts, their contribution to the acquisition and spread of antibiotic resistance genes in the environment has not been extensively studied. The environment is continually exposed to a wide variety of pollutants from anthropogenic sources, which may
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Cadaverine reverse transporter (CadB protein) contributes to the virulence of Aeromonas veronii TH0426. Int. Microbiol. (IF 1.833) Pub Date : 2020-01-16 LuoTao Tao,YuanHuan Kang,Lei Zhang,Qiumei Shi,Ying Li,Tonglei Wu,Aidong Qian,WuWen Sun,Xiaofeng Shan
Aeromonas veronii is one of the main pathogens causing sepsis and ulcer syndrome in freshwater fish. Analysis of the results of epidemiological investigations in recent years has revealed that the virulence of A. veronii and its tolerance to drugs have been increasing year by year. Currently, most of the research on A. veronii focuses on its isolation, identification, and drug susceptibility, whereas
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Metagenomic analyses uncover the differential effect of azide treatment on bacterial community structure by enriching a specific Cyanobacteria present in a saline-alkaline environmental sample. Int. Microbiol. (IF 1.833) Pub Date : 2020-01-14 José Félix Aguirre-Garrido,Francisco Martínez-Abarca,Daniel Montiel-Lugo,Luis Mario Hernández-Soto,Hugo Ramírez-Saad
Treatment of environmental samples under field conditions may require the application of chemical preservatives, although their use sometimes produces changes in the microbial communities. Sodium azide, a commonly used preservative, is known to differentially affect the growth of bacteria. Application of azide and darkness incubation to Isabel soda lake water samples induced changes in the structure
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Influence of salt stress on the rhizosphere soil bacterial community structure and growth performance of groundnut (Arachis hypogaea L.). Int. Microbiol. (IF 1.833) Pub Date : 2020-01-13 Yang Xu,Guanchu Zhang,Hong Ding,Dunwei Ci,Liangxiang Dai,Zhimeng Zhang
Soil salinity is regarded as severe environmental stress that can change the composition of rhizosphere soil bacterial community and import a plethora of harms to crop plants. However, relatively little is known about the relationship between salt stress and root microbial communities in groundnuts. The goal of this study was to assess the effect of salt stress on groundnut growth performance and rhizosphere
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Improvement of vincamine production of endophytic fungus through inactivated protoplast fusion. Int. Microbiol. (IF 1.833) Pub Date : 2020-01-11 Shijie Xu,Na Ren,Jiajia Liu,Yiqiang Wu,Guangming Yuan
Improvement of the production of vincamine in endophytic fungus VINI-7 was performed by using the inactivated protoplast fusion method. The preparation conditions of protoplasts were optimized by systematic trials with various parameters, and inactivated protoplast fusion was subsequently performed. The mycelium in logarithmic growth phase was treated with 1500 U/mL lywallzyme, 1500 U/mL lysozyme,
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The signaling mechanisms involved in the dimorphic phenomenon of the Basidiomycota fungus Ustilago maydis. Int. Microbiol. (IF 1.833) Pub Date : 2020-01-08 José Ruiz-Herrera,Fernando Pérez-Rodríguez,John Velez-Haro
In the present manuscript, we describe the mechanisms involved in the yeast-to-hypha dimorphic transition of the plant pathogenic Basidiomycota fungus Ustilago maydis. During its life cycle, U. maydis presents two stages: one in the form of haploid saprophytic yeasts that divide by budding and the other that is the product of the mating of sexually compatible yeast cells (sporidia), in the form of
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The phosphoenolpyruvate-pyruvate-oxaloacetate node genes and enzymes in Streptomyces coelicolor M-145. Int. Microbiol. (IF 1.833) Pub Date : 2020-01-03 Reneé Llamas-Ramírez,Toshiko Takahashi-Iñiguez,María Elena Flores
The phosphoenolpyruvate-pyruvate-oxaloacetate node is a major branch within the central carbon metabolism and acts as a connection point between glycolysis, gluconeogenesis, and the TCA cycle. Phosphoenolpyruvate carboxylase, pyruvate carboxylase, phosphoenolpyruvate carboxykinase, malic enzymes, and pyruvate kinase, among others, are enzymes included in this node. We determined the mRNA levels and
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An ancestral genomic locus in Mycobacterium tuberculosis clinical isolates from India hints the genetic link with Mycobacterium canettii. Int. Microbiol. (IF 1.833) Pub Date : 2020-01-02 Suma Sarojini,Sathish Mundayoor
BackgroundTuberculosis remains a worldwide public health emergency. To better understand M. tuberculosis and to identify genomic variations characteristic to the Indian clinical isolates by a low-cost method, a genomic subtractive hybridization between M. tuberculosis H37Rv and a clinical isolate from South India was performed. ResultsThis revealed a novel 0.4-kb subtractive fragment which was used
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Mining the roots of various species of the halophyte Suaeda for halotolerant nitrogen-fixing endophytic bacteria with the potential for promoting plant growth. Int. Microbiol. (IF 1.833) Pub Date : 2020-01-02 Frashad Alishahi,Hossein Ali Alikhani,Nayer Azam Khoshkholgh-Sima,Hassan Etesami
Saline area may tend to be a productive land; however, many of salt-affected soils have nitrogen limitation and depend on plant-associated diazotrophs as their source of ‘new’ nitrogen. Herein, a total of 316 salinity tolerant nitrogen-fixing endophytic bacteria were isolated from roots of the halophyte Suaeda sp. sampled from 22 different areas of Iran to prepare the collection of nitrogen-fixing
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Radio frequency-induced superoxide accumulation affected the growth and viability of Saccharomyces cerevisiae. Int. Microbiol. (IF 1.833) Pub Date : 2020-01-02 Qing Li,Miao Tian,Jie Teng,Peng Gao,Bruce Qing Tang,Hong Wu
With the development of the electric technologies, the biological effects of electromagnetic fields (EMF) were widely studied. However, the results remain controversial and the biophysical mechanisms are still unknown. To our knowledge, little studies pay attention to the radio frequency (RF) of 2.6–5 MHz. In the present study, we investigated the effect of these radio frequencies on the growth and
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Performance and microbial diversity of denitrifying biofilms on polyurethane foam coupled with various solid carbon sources for nitrate-rich water purification. Int. Microbiol. (IF 1.833) Pub Date : 2020-01-02 Shuangyu Pi,Jingya Sun,Lijuan Feng,Jiaheng Zhou
This study investigated the performance and microbial communities of denitrifying biofilms on polyurethane foam coupled with various solid carbon sources of acid- and alkali-pretreated rice straw and rice husk. Results showed that acid and alkali-pretreated rice straw both had higher TOC release rates (0.041–0.685 mg g−1 day−1) than those of rice husk (0.019–0.160 mg g−1 day−1) over a month, while
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Recent progress on elucidating the molecular mechanism of plasmid-mediated colistin resistance and drug design. Int. Microbiol. (IF 1.833) Pub Date : 2019-12-23 Jindan Kai,Sheng Wang
Antibiotic resistance is a growing global challenge to public health. Polymyxin is considered to be the last-resort antibiotic against most gram-negative bacteria. Recently, discoveries of a plasmid-mediated, transferable mobilized polymyxin resistance gene (mcr-1) in many countries have heralded the increased threat of the imminent emergence of pan-drug-resistant super bacteria. MCR-1 is an inner
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The putative transcriptional regulator STM14_3563 facilitates Salmonella Typhimurium pathogenicity by activating virulence-related genes. Int. Microbiol. (IF 1.833) Pub Date : 2019-12-13 Xiaorui Song,Huan Zhang,Xiaoqian Liu,Jian Yuan,Peisheng Wang,Runxia Lv,Bin Yang,Di Huang,Lingyan Jiang
Salmonella enterica serovar Typhimurium (S. Typhimurium) is an important gram-negative intracellular pathogen that infects humans and animals. More than 50 putative regulatory proteins have been identified in the S. Typhimurium genome, but few have been clearly defined. In this study, the physiological function and regulatory role of STM14_3563, which encodes a ParD family putative transcriptional
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Integrated selection and identification of bacteria from polluted sites for biodegradation of lipids. Int. Microbiol. (IF 1.833) Pub Date : 2019-12-11 Pedro D Teixeira,Vitor S Silva,Rogério Tenreiro
Wastewater treatment plants face major social concern towards removal of problematic pollutants such as fat oils and grease (FOG). In this context, the main objective of the present work was to select natural bacterial isolates from different polluted sites and evaluate them comparatively to isolates from commercial products, for improved bioremediation strategies and bioaugmentation. In total, 196
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Screening of Gastrointestinal Lipase Inhibitors Produced by Microorganisms Isolated from Soil and Lake Sediments. Int. Microbiol. (IF 1.833) Pub Date : 2019-12-10 Maria Angeles Camacho-Ruiz,Enrique Ordaz,Manuel R Kirchmayr,Hugo Esquivel-Solís,Ali Asaff-Torres,Juan Carlos Mateos-Díaz,Frédéric Carriѐre,Jorge A Rodríguez
Gastrointestinal lipase inhibitors are molecules of pharmaceutical interest due to their use as anti-obesity drugs. In this study, forty strains isolated from soil and sediments were identified with the ability to produce inhibition of gastrointestinal lipase activity. The biomass extract of these strains showed at least 50% inhibition in the hydrolysis of tributyrin by recombinant human pancreatic
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Treatments with native Coleus forskohlii endophytes improve fitness and secondary metabolite production of some medicinal and aromatic plants Int. Microbiol. (IF 1.833) Pub Date : 2019-12-10 Anthati Mastan; Chikkarasanahalli Shivegowda Vivek Babu; Channayya Hiremath; Kalavagunta Venkata Naga Satya Srinivas; Arigari Niranjan Kumar; Jonnala Kotesh Kumar
Endophytes have been shown to play a crucial role in determining the fitness of host plant during their association, yet the cross-functional effect of endophytes of one plant on another plant remains largely uncharacterized. In this study, we attempt to analyze the effect of native endophytes of Coleus forskohlii (Phialemoniopsis cornearis (SF1), Macrophomina pseudophaseolina (SF2), and Fusarium redolens
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Isolation of xylose-assimilating yeasts and optimization of xylitol production by a new Meyerozyma guilliermondii strain. Int. Microbiol. (IF 1.833) Pub Date : 2019-12-07 Fernando Augusto da Silveira,Tatiana Alves Rigamonte Fernandes,Caio Roberto Soares Bragança,Thércia Rocha Balbino,Raphael Hermano Santos Diniz,Flávia Maria Lopes Passos,Wendel Batista da Silveira
Production of xylitol from lignocellulosic biomass is of interest to modern biorefineries, because this biomass should be processed into a spectrum of chemicals (bio-based products) and not only energy. The isolation of new yeast strains capable of efficiently converting xylose into xylitol and withstanding inhibitors released from biomass hydrolysis can contribute to making its production feasible
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Revealing the bacterial community profiles during the degradation of acetone, propionic and hexanoic acids-components of wastewater from the Fischer-Tropsch process. Int. Microbiol. (IF 1.833) Pub Date : 2019-11-22 Thapelo P Malematja,Grace N Ijoma,Ramganesh Selvarajan,Tonderayi Matambo
The Fischer-Tropsch (F-T) process for production of fuels is entrenched in several countries’ approach to meeting energy demands. However, the clean water deficit associated with the down-stream processes has made it necessary to explore bioremediation methods to ameliorate the consequences of its use. In this study, a consortium of bacteria was utilized for determination of biodegradation and removal
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New trichrome stains identify cysts of Colpodella sp. (Apicomplexa) and Bodo caudatus. Int. Microbiol. (IF 1.833) Pub Date : 2019-11-20 Tobili Y Sam-Yellowe,Kush Addepalli,Raghavendra Yadavalli,John W Peterson
Colpodella species are free-living close relatives of apicomplexans that were recently reported to cause red blood cell infection in an immunocompromised human host and in a tick-borne human infection resulting in neurological symptoms. Unambiguous identification of the life cycle stages of Colpodella sp. using routine stains for light microscopy will aid rapid diagnosis in infections. Similarly, cells
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Microbial use for azo dye degradation-a strategy for dye bioremediation. Int. Microbiol. (IF 1.833) Pub Date : 2019-11-18 Mehvish Ajaz,Sana Shakeel,Abdul Rehman
Azo dyes are aromatic compounds with one to many –N=N– groups as well as the leading class of synthetic dyes utilised in commercial solicitations. Azo dyes, released in the environment through textile effluents, have hazardous effects on the aquatic as well as human life. Their persistence and discharge into the environment are becoming a global concern; thus, the remediation of these contaminants
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Growth conditions, physiological properties, and selection of optimal parameters of biodegradation of anticancer drug daunomycin in industrial effluents by Bjerkandera adusta CCBAS930. Int. Microbiol. (IF 1.833) Pub Date : 2019-11-12 Teresa Korniłłowicz-Kowalska,Kamila Rybczyńska-Tkaczyk
The study characterizes the anamorphic Bjerkandera adusta strain CCBAS 930, including growth conditions, physiological properties, and enzymatic activities related to basic metabolism and specific properties coupled with the fungal secondary metabolism. It was established that the fungus grows in a wide pH range (3.5–7.5), up to 3% of salt concentration and a temperature of 5–30 °C. Media rich in natural
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Fungal Signaling: from Homeostasis to Pathogenesis. Int. Microbiol. (IF 1.833) Pub Date : 2019-08-13 Humberto Martín