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Comparative analysis of rabies pathogenic and vaccine strains detection by RIG-I-like receptors Microbes Infect. (IF 5.8) Pub Date : 2024-03-09 Wahiba Aouadi, Valérie Najburg, Rachel Legendre, Hugo Varet, Lauriane Kergoat, Frédéric Tangy, Florence Larrous, Anastassia V. Komarova, Hervé Bourhy
Rabies virus (RABV) is a lethal neurotropic virus that causes 60,000 human deaths every year globally. RABV infection is characterized by the suppression of the interferon (IFN)-mediated antiviral response. However, molecular mechanisms leading to RABV sensing by RIG-I-like receptors (RLR) that initiates IFN signaling currently remain elusive. Here, we showed that RABV RNAs are primarily recognized
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Efficacy of 233 nm LED far UV-C-radiation against clinically relevant bacterial strains in the phase 2/ step 2 in vitro test on basis of EN 14561 and on an epidermis cell model Microbes Infect. (IF 5.8) Pub Date : 2024-03-09 Claudia Sicher, Nevin Opitz, Pia Elen Becker, Neysha Lobo Ploch, Johannes Schleusener, Michael Kneissl, Axel Kramer, Paula Zwicker
Healthcare-acquired infections and overuse of antibiotics are a common problem. Rising emergence of antibiotic and antiseptic resistances requires new methods of microbial decontamination or decolonization as the use of far-UV-C radiation. The microbicidal efficacy of UV-C radiation (222 nm, 233 nm, 254 nm) was determined in a quantitative carrier test and on 3D-epidermis models against and . To mimic
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Systems vaccinology studies – achievements and future potential Microbes Infect. (IF 5.8) Pub Date : 2024-03-07 Jamie A. Sugrue, Darragh Duffy
Human responses to vaccination are variable both within and between populations. Systems vaccinology, which is the application of multi-omics technologies to vaccine studies, seeks to understand such variation and predict responses to optimise vaccine strategies. Here we outline new approaches to systems vaccinology, focusing on the incorporation of additional cohorts, endpoints and technologies.
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Paired qualitative and quantitative analysis of bacterial microcolonies in the tonsils of patients with tonsillar hyperplasia Microbes Infect. (IF 5.8) Pub Date : 2024-03-05 Jackson Teh, Kristi Biswas, Sharon Waldvogel-Thurlow, David Broderick, Sita Tarini Clark, James Johnston, Brett Wagner Mackenzie, Richard Douglas
The discovery of bacterial microcolonies in tonsillar tissue of patients with tonsillar hyperplasia has raised the question of their role in provoking the local immune response. Tonsils collected from patients undergoing tonsillectomy were stained for three clinically relevant bacterial taxa and lymphocytes. The bacterial composition and abundance of microcolonies was investigated using a combination
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Organoids and organ-on-chip technology for investigating host-microorganism interactions Microbes Infect. (IF 5.8) Pub Date : 2024-03-04 Remigiusz Walocha, MinHee Kim, Jérôme Wong-Ng, Samy Gobaa, Nathalie Sauvonnet
Recent advances in organoid and organ-on-chip (OoC) technologies offer an unprecedented level of tissue mimicry. These models can recapitulate the diversity of cellular composition, 3D organization, and mechanical stimulation. These approaches are intensively used to understand complex diseases. This review focuses on the latest advances in this field to study host-microorganism interactions.
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Comprehensive analysis of a novel subtype of immune microenvironment-derived HPV-infected colorectal cancer Microbes Infect. (IF 5.8) Pub Date : 2024-02-27 Jianlong Zhou, Yongfeng Liu, Yucheng Zhang, Fa Ling, Jiabin Zheng, Xueqing Yao, Zejian Lyu, Huolun Feng, Yong Li
The current study proposed a novel subtype, Human papillomavirus (HPV)-infected colorectal cancer (CRC), to understand the impact of HPV on CRC. We assessed the prevalence and clinical implications of HPV in CRC by integrating a single cohort in Guangdong Provincial People's Hospital and public datasets. Differential gene, pathway enrichment, and immune infiltration analysis were conducted to explore
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Prevotella, a dominant bacterium in young people with stage Ⅲ periodontitis, related to the arachidonic acid metabolism pathway Microbes Infect. (IF 5.8) Pub Date : 2024-02-27 Qin Ye, Yaqiong Zhao, Jie Zhao, Zeyue Ouyang, Yao Feng, Jing Hu, Xiaolin Su, Ningxin Chen, Yun Chen, Li Tan, Yunzhi Feng, Yue Guo
As periodontitis progresses, the oral microbiome changes dynamically. The aim of this study is to evaluate the dominant bacteria of adults with stage III periodontitis and investigate potential pathways related to the dominant bacteria. 16S rRNA sequencing was carried out to detect the differences in the oral microbiome between adult with stage Ⅰ and stage Ⅲ periodontitis and find the dominant bacteria
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Unraveling the intricacies of host-pathogen interaction through single-cell genomics Microbes Infect. (IF 5.8) Pub Date : 2024-02-16 Emanuele Gioacchino, Koen Vandelannoote, Anthony A. Ruberto, Jean Popovici, Tineke Cantaert
Single-cell genomics provide researchers with tools to assess host-pathogen interactions at a resolution previously inaccessible. Transcriptome analysis, epigenome analysis, and immune profiling techniques allow for a better comprehension of the heterogeneity underlying both the host response and infectious agents. Here, we highlight technological advancements and data analysis workflows that increase
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SUBCELLULAR PARTICLES for CHARACTERIZATION of HOST-PARASITE INTERACTIONS Microbes Infect. (IF 5.8) Pub Date : 2024-02-16 Ewa Kozela, Paula Meneghetti, Neta Regev-Rudzki, Ana Claudia Torrecilhas, Ziv Porat
Parasitic diseases remain a major global health problem for humans. Parasites employ a variety of strategies to invade and survive within their hosts and to manipulate host defense mechanisms, always in the pathogen's favor. Extracellular vesicles (EVs), membrane-bound nanospheres carrying a variety of bioactive compounds, were shown to be released by the parasites during all stages of the infection
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Kinetic and proteomic studies in milk show distinct patterns among major Listeria monocytogenes clones Microbes Infect. (IF 5.8) Pub Date : 2024-02-11 Alba Espí-Malillos, Carla Palacios-Gorba, Inmaculada López-Almela, Pilar Ruiz-García, María Carmen López-Mendoza, Francisco García-Del Portillo, M Graciela Pucciarelli, Juan J. Quereda
, a contaminant of raw milk, includes hypervirulent clonal complexes (CC) like CC1, CC4, and CC6, highly overrepresented in dairy products when compared to other food types. Whether their higher prevalence in dairy products is the consequence of a growth advantage in this food remains unknown. We examined growth kinetics of five isolates (CC1, CC4, CC6, CC9, and CC121) at 37 and 4 °C in ultra-high
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Can Lacticaseibacillus rhamnosus CRL1505 postbiotic improve emergency myelopoiesis in immunocompromised mice? Microbes Infect. (IF 5.8) Pub Date : 2024-02-09 Andrés Gramajo Lopez, Brenda Vasile, Yanina Kolling, Maximiliano Ivir, Florencia Gutiérrez, Susana Alvarez, Susana Salva
We evaluated whether viable and non-viable CRL1505 (Lr05V or Lr05NV, respectively) was able to improve emergency myelopoiesis induced by (Sp) infection.
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Various mutations in icaR, the repressor of the icaADBC locus, occur in mucoid Staphylococcus aureus isolates recovered from the airways of people with cystic fibrosis Microbes Infect. (IF 5.8) Pub Date : 2024-02-04 Bianca Schwartbeck, Christine H. Rumpf, Robert Jonathan Hait, Timo Janssen, Susanne Deiwick, Vera Schwierzeck, Alexander Mellmann, Barbara C. Kahl
is one of the major pathogens isolated from the airways of people with cystic fibrosis (pwCF). Recently, we described a mucoid phenotype from respiratory specimens of pwCF, which constitutively overproduced biofilm that consisted of polysaccharide intercellular adhesin (PIA) due to a 5bp-deletion (5bp-del) in the intergenic region of the intercellular adhesin () locus.
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Gut microb-aging and its relevance to frailty aging Microbes Infect. (IF 5.8) Pub Date : 2024-02-04 Guillaume Le Cosquer, Nathalie Vergnolle, Jean-Paul Motta
This review explores ‘microb-aging’ in the gut and its potential link to frailty aging. We explore this connection through alterations in microbiota's taxonomy and metabolism, as well as with concepts of ecological resilience, pathobionts emergence, and biogeography. We examine microb-aging in interconnected body organs, emphasizing the bidirectional relationship with ‘inflammaging’. Finally, we discuss
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Extracellular vesicles in parasitic diseases – from pathogenesis to future diagnostic tools Microbes Infect. (IF 5.8) Pub Date : 2024-02-04 Saulo Almeida Menezes, Tiana Tasca
Parasitic diseases are still a major public health problem especially among individuals of low socioeconomic status in underdeveloped countries. In recent years it has been demonstrated that parasites can release extracellular vesicles that participate in the host-parasite communication, immune evasion, and in governing processes associated with host infection. Extracellular vesicles are membrane-bound
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Glycerol metabolic repressor GlpR contributes to Streptococcus suis oxidative stress resistance and virulence Microbes Infect. (IF 5.8) Pub Date : 2024-02-02 Zijing Liang, Jiaxuan Lu, Yinli Bao, Xiang Chen, Huochun Yao, Zongfu Wu
Bacterial DeoR family transcription regulators regulate multiple physiological processes. Little is known about the function of DeoR family regulators in streptococci. Here, we identified a novel DeoR family regulator, GlpR, from , a pathogen causing severe diseases in pigs and humans. GlpR was involved in glycerol utilization and exhibited specific signature residues at positions 30-31 (KV) which
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Role of vaginal microbiota in idiopathic infertility: a prospective study Microbes Infect. (IF 5.8) Pub Date : 2024-02-02 Chitrakshi Chopra, Vinay Kumar, Manoj Kumar, Indu Bhushan
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Candida albicans increases the aerobic glycolysis and activates MAPK–dependent inflammatory response of liver sinusoidal endothelial cells Microbes Infect. (IF 5.8) Pub Date : 2024-01-29 Leire Aparicio-Fernandez, Aitziber Antoran, Maialen Areitio, Oier Rodriguez-Erenaga, Leire Martin-Souto, Idoia Buldain, Joana Márquez, Aitor Benedicto, Beatriz Arteta, Aize Pellon, David L. Moyes, Aitor Rementeria, Andoni Ramirez-Garcia
The liver, and more specifically, the liver sinusoidal endothelial cells, constitute the beginning of one of the most important responses for the elimination of hematogenously disseminated Candida albicans. Therefore, we aimed to study the mechanisms involved in the interaction between these cells and C. albicans. Transcriptomics-based analysis showed an increase in the expression of genes related
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Lineage classification and selective site identification of Orthoebolavirus zairense Microbes Infect. (IF 5.8) Pub Date : 2024-01-24 Jie Fang, Zhijian Zhou, Shuofeng Yuan, Ye Qiu, Xing-Yi Ge
As the high pathogenic species of Filoviridae virus family, Orthoebolavirus zairense (EBOV) shows frequent outbreaks in human in recently years since its first emerging in 1976 in Democratic Republic of the Congo (COD), bringing ongoing risks and burden on public health safety. Here, the phylogenetic relationship among major outbreaks was analyzed. The results showed that EBOV isolates could be divided
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Tick-borne encephalitis virus modulates sphingolipid and phospholipid metabolism in infected human neuronal cells Microbes Infect. (IF 5.8) Pub Date : 2024-01-24 Pavlína Šimečková, Josef Slavík, Andrea Fořtová, Ivana Huvarová, Lucie Králiková, Michal Stefanik, Pavel Svoboda, Daniel Ruzek, Miroslav Machala
The life cycle of enveloped viruses is closely linked to host-cell lipids. However, changes in lipid metabolism during infections with the tick-borne encephalitis virus (TBEV) have not been described. TBEV is a medically important orthoflavivirus, which is endemic to many parts of Europe and Asia. In the present study, we performed targeted lipidomics with HPLC-MS/MS to evaluate changes in phospholipid
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Elevated serum level of progranulin is associated with increased mortality in critically ill patients with candidemia Microbes Infect. (IF 5.8) Pub Date : 2024-01-20 Jiayu Liu, Yue Li, Yuhan Liu, Renlin Yu, Yibing Yin, Xiaofei Lai, Banglao Xu, Ju Cao
Candidemia is a severe disease with high mortality in both intensive care unit (ICU) and non-ICU settings. Considering that progranulin (PGRN) is a potential therapeutic target for the candidemia caused by C. albicans, we determined the serum level of PGRN after candidemia and evaluated its association with mortality. A retrospective discovery cohort (62 patients) and a validation cohort (70 patients)
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Cell-free supernatants from Lactobacillus strains exert antibacterial, antibiofilm, and antivirulence activity against Pseudomonas aeruginosa from cystic fibrosis patients. Microbes Infect. (IF 5.8) Pub Date : 2024-01-17 Arianna Pompilio, Esingül Kaya, Veronica Lupetti, Elisa Catelli, Marta Bianchi, Giuseppantonio Maisetta, Semih Esin, Giovanni Di Bonaventura, Giovanna Batoni
Chronic lung infections caused by Pseudomonas aeruginosa play a significant role in the mortality and morbidity of cystic fibrosis (CF) patients. The widespread bacterial resistance to conventional antimicrobials demands identifying new strategies to complement or replace current antibiotic therapies. In this study, we evaluated the antibacterial, antibiofilm, and antivirulence properties of cell-free
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Association of CC-Chemokine Ligand-2 gene Polymorphisms with Leprosy Reactions Microbes Infect. (IF 5.8) Pub Date : 2024-01-18 Sanjay Kumar, Keshar Kunja Mohanty, Vandana Singh, Mohan Natarajan, Mamta Arora, Joy Kumar Chakma, Srikanth Prasad Tripathy
Background C-C motif chemokine ligand 2, a gene that codes for a protein involved in inflammation. Certain SNPs in the CCL2 gene have been studied for their potential associations with susceptibility to various diseases. These SNPs may affect the production and function of the CCL2 protein, which is involved in the recruitment of immune cells to sites of inflammation. Variations in CCL2 may influence
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Blocking Pathogenic Leptospira Invasion with Aptamer Molecules Targeting Outer Membrane LipL32 Protein Microbes Infect. (IF 5.8) Pub Date : 2024-01-14 Shen-Hsing Hsu, Huang-Yu Yang, Chia-Chen Chang, Shou-Kuan Tsai, Chien Li, Ming-Yang Chang, Yi-Ching Ko, Li-Fang Chou, Chung-Ying Tsai, Ya-Chung Tian, Chih-Wei Yang
This study aimed to develop aptamers targeting LipL32, a most abundant lipoprotein in pathogenic Leptospira, to hinder bacterial invasion. The objectives were to identify high-affinity aptamers through SELEX and evaluate their specificity and inhibitory effects. SELEX was employed to generate LipL32 aptamers (L32APs) over 15 rounds of selection. L32APs' binding affinity and specificity for pathogenic
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MicroRNAs correlate with bacillary index and genes associated to cell death processes in leprosy Microbes Infect. (IF 5.8) Pub Date : 2024-01-14 Nadja de Lima Santana, Lucas Neves de Farias, Tainã Lago, Thyago Leal-Calvo, Paulo Roberto Lima Machado, Léa Cristina Castellucci
Mycobacterium leprae infects skin and peripheral nerves causing a broad of clinical forms. MicroRNAs (miRNAs) control immune mechanisms such as apoptosis, autophagy as well as to target genes leading to abnormal proliferation, metastasis, and invasion of cells. Herein we evaluated miRNAs expression for leprosy phenotypes in biopsies obtained from patients with and without reactions. We also correlated
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NMR spectroscopy can help accelerate antiviral drug discovery programs Microbes Infect. (IF 5.8) Pub Date : 2024-01-09 Steven R. LaPlante, Pascale Coric, Serge Bouaziz, Tanos C.C. França
Small molecule drugs have an important role to play in combating viral infections, and biophysics support has been central for contributing to the discovery and design of direct acting antivirals. Perhaps one of the most successful biophysical tools for this purpose is NMR spectroscopy when utilized strategically and pragmatically within team workflows and timelines. This report describes some clear
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Present and future perspectives on mass spectrometry for clinical microbiology Microbes Infect. (IF 5.8) Pub Date : 2024-01-09 Megan S. Gant, Julia Chamot-Rooke
In the last decade, MALDI-TOF Mass Spectrometry (MALDI-TOF MS) has been introduced and broadly accepted by clinical laboratory laboratories throughout the world as a powerful and efficient tool for rapid microbial identification. During the MALDI-TOF MS process, microbes are identified using either intact cells or cell extracts. The process is rapid, sensitive, and economical in terms of both labor
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Prevalence of co-infection with human papillomavirus and Chlamydia trachomatis and risk factors associated with cervical cancer in Congolese women Microbes Infect. (IF 5.8) Pub Date : 2023-12-30 Dorine Florence Luthera Ngombe Mouabata, Anicet Luc Magloire Boumba, Norvi Rigobert Bienvenu Massengo, Freddy Saturnin Pouki, Donatien Moukassa, Moulay Mustapha Ennaji
The human papillomavirus (HPV) is one of the most frequently diagnosed viruses in developing countries. (CT) is an important cofactor in HPV-induced cervical cancer.
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Elemental sulfur enhances the anti-fungal effect of Lacticaseibacillus rhamnosus Lcr35 Microbes Infect. (IF 5.8) Pub Date : 2023-12-30 Manjyot Kaur, Sylvie Miquel, Laurence Ollivier-Nakusi, Claudia Thoral, Marjolaine Vareille-Delarbre, Clara Bekirian, Christophe d’Enfert, Thierry Fontaine, Karine Roget, Christiane Forestier
Lcr35 is a well-known bacterial strain whose efficiency in preventing recurrent vulvovaginal candidiasis has been largely demonstrated in clinical trials. The presence of sodium thiosulfate (STS) has been shown to enhance its ability to inhibit the growth of strains. In this study, we confirmed that Lcr35 has a fungicidal effect not only on the planktonic form of but also on other life forms such as
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Genomic features and pathophysiological impact of a multidrug-resistant Staphylococcus warneri variant in murine mastitis Microbes Infect. (IF 5.8) Pub Date : 2023-12-26 M. Nazmul Hoque, Golam Mahbub Faisal, Ziban Chandra Das, Tahsin Islam Sakif, Mamun Al Mahtab, M. Anwar Hossain, Tofazzal Islam
Non-aureus staphylococci (NAS) represent a major etiological agent in dairy animal mastitis, yet their role and impact remain insufficiently studied. This study aimed to elucidate the genomic characteristics of a newly identified multidrug-resistant NAS strain, specifically G1M1F, isolated from murine feces in an experimental mastitis model. Surprisingly, NAS species accounted for 54.35 % of murine
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Unravelling the potential of Triflusal as an anti-TB repurposed drug by targeting replication protein DciA Microbes Infect. (IF 5.8) Pub Date : 2023-12-23 Waseem Ali, Salma Jamal, Rishabh Gangwar, Faraz Ahmed, Isha Pahuja, Rahul Sharma, Ved Prakash Dwivedi, Meetu Agarwal, Sonam Grover
The increasing prevalence of drug-resistant Tuberculosis (TB) is imposing extreme difficulties in controlling the TB infection rate globally, making treatment critically challenging. To combat the prevailing situation, it is crucial to explore new anti-TB drugs with a novel mechanism of action and high efficacy. The ()DciA is an essential protein involved in bacterial replication and regulates its
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Transcriptional regulation of suppressors of cytokine signaling during infection with Mycobacterium tuberculosis in human THP-1-derived macrophages and in mice Microbes Infect. (IF 5.8) Pub Date : 2023-12-21 Trisha Roy, Anuradha Seth, Hasham Shafi, D.V. Siva Reddy, Sunil Kumar Raman, J.V.U.S. Chakradhar, Sonia Verma, Reena Bharti, Lubna Azmi, Lipika Ray, Amit Misra
(Mtb) infection leads to upregulation of Suppressors of Cytokine signaling (SOCS) expression in host macrophages (Mϕ). SOCS proteins inhibit cytokine signaling by negatively regulating JAK/STAT. We investigated this host-pathogen dialectic at the level of transcription. We used phorbol-differentiated THP-1 Mϕ infected with Mtb to investigate preferential upregulation of some SOCS isoforms that are
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Mycobacterium leprae is able to infect adipocytes, inducing lipolysis and modulating the immune response Microbes Infect. (IF 5.8) Pub Date : 2023-12-21 Sabrina Alves dos Reis, Jessica Dias Gonçalves, Karoline dos Anjos Lima, Thaina Magalhaes Demaria, Emylle Costa-Bartuli, Tiago Araujo Gomes, Meydson Benjamim Carvalho Corrêa, Georgia Correa Atella, Mauro Sola-Penna, Patricia Sammarco Rosa, Maria Cristina Vidal Pessolani, Jyothi Nagajyothi, Flavio Alves Lara
Leprosy is a chronic infectious disease caused by the intracellular bacillus (), which is known to infect skin macrophages and Schwann cells. Although adipose tissue is a recognized site of infection, its role in the histopathology of leprosy was, until now, unknown. We analyzed the capacity to infect and persist inside adipocytes, characterizing the induction of a lipolytic phenotype in adipocytes
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SP-R210 isoforms of Myosin18A modulate endosomal sorting and recognition of influenza A virus infection in macrophages Microbes Infect. (IF 5.8) Pub Date : 2023-12-21 Eric Yau, Linlin Yang, Yan Chen, Todd M. Umstead, Anne E. Stanley, E. Scott Halstead, Chintan K. Gandhi, Jonathan W. Yewdell, Zissis C. Chroneos
Influenza A virus (IAV) infection causes acute and often lethal inflammation in the lung. The role of macrophages in this adverse inflammation is partially understood. The surfactant protein A receptor 210 (SP-R210) consists of two isoforms, a long (L) SP-R210 and a short (S) SP-R210 isoform encoded by alternative splicing of the myosin 18A gene. We reported that disruption of SP-R210 enhances cytosolic
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Human gut microbiota from hepatitis B virus-infected individuals is associated with reduced triglyceride level in mice: faecal transplantation study Microbes Infect. (IF 5.8) Pub Date : 2023-12-20 Han-Na Kim, Hae Suk Cheong, Bomi Kim, Won Sohn, Yong Kyun Cho, Min-Jung Kwon, Juhee Kim, Youngmi Song, Eun-Jeong Joo
Chronic hepatitis B virus (HBV) infection is associated with a reduced risk of dyslipidaemia. Using a human faecal microbiota transplantation (FMT), we compared changes in gut microbiota and lipid profiles in mice transplanted with human faeces from HBV-infected and non-infected individuals. A total of 19 mice received human FMT from four HBV-infected individuals and were categorised into the HBV-positive
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Bioactives from medicinal herb against bedaquiline resistant tuberculosis: removing the dark clouds from the horizon Microbes Infect. (IF 5.8) Pub Date : 2023-12-19 Rahul Singh, Souvik Manna, Hemraj Nandanwar, Rituraj Purohit
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Rab27a via Its effector JFC1 Localizes to Anaplasma Inclusions and Promotes Anaplasma Proliferation in Leukocytes Microbes Infect. (IF 5.8) Pub Date : 2023-12-16 Weiyan Huang, Mingqun Lin, Yasuko Rikihisa
Anaplasma phagocytophilum is an obligatory intracellular bacterium that causes tick-borne zoonosis called human granulocytic anaplasmosis. Mechanisms by which Anaplasma replicates inside of the membrane-bound compartment called “inclusion” in neutrophils is incompletely understood. A small GTPase Rab27a is found in the secretory granules and multivesicular endosomes. In this study we found Rab27a-containing
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Myeloid derived suppressor cells potentiate virus-specific memory CD8+ T cell response Microbes Infect. (IF 5.8) Pub Date : 2023-12-14 Roman Sarkar, Mohammad Shaaz, Sharvan Sehrawat
How therapeutically administered myeloid derived suppressor cells (MDSCs) modulate differentiation of virus-specific CD8 T cell was investigated. generated MDSCs from bone marrow precursors inhibited the expansion of stimulated CD8 T cells but the effector cells in the recipients of MDSCs showed preferential memory transition during Influenza A virus (IAV) or an α− (Herpes Simplex Virus) as well as
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The functions of EF-hand proteins from host and zoonotic pathogens Microbes Infect. (IF 5.8) Pub Date : 2023-12-09 Wei Zuo, Mingxing Tian, Jingjing Qi, Guangdong Zhang, Jiangang Hu, Shaohui Wang, Yanqing Bao
EF-hand proteins not only regulate biological processes, but also influence immunity and infection. In this review, we summarize EF-hand proteins’ functions in host and zoonotic pathogens, with details in structures, Ca2+ affinity, downstream targets and functional mechanisms. Studies entitled as EF-hand-related but with less solid features were also discussed. We believe it could raise cautions and
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Comparative genome analysis of Streptococcus suis serotype 5 strains from humans and pigs revealed pathogenic potential of virulent, antimicrobial resistance, and genetic relationship Microbes Infect. (IF 5.8) Pub Date : 2023-12-07 Anusak Kerdsin, Rujirat Hatrongjit, Thidathip Wongsurawat, Piroon Jenjaroenpun, Han Zheng, Peechanika Chopjitt, Parichart Boueroy, Nahuel Fittipaldi, Mariela Segura, Marcelo Gottschalk
Streptococcus suis is a causative agent of swine and human infections. Genomic analysis indicated that eight S. suis serotype 5 strains recovered from human patients and pigs carried many virulence-associated genes and markers defining pathogenic pathotypes. The strains were sequence types diverse and clustered within either minimum core genome group 3 (MCG-3) or MCG-7-3. Almost all the serotype 5
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Leptospiral lipopolysaccharide dampens inflammation through upregulation of autophagy adaptor p62 and NRF2 signaling in macrophages Microbes Infect. (IF 5.8) Pub Date : 2023-12-09 Delphine Bonhomme, Ignacio Santecchia, Pedro Escoll, Stylianos Papadopoulos, Frédérique Vernel-Pauillac, Ivo G. Boneca, Catherine Werts
are pathogenic bacteria responsible for leptospirosis, a worldwide zoonosis. All vertebrates can be infected, and some species like humans are susceptible to the disease whereas rodents such as mice are resistant and become asymptomatic renal carriers. Leptospires are stealth bacteria that are known to escape several immune recognition pathways and resist killing mechanisms. We recently published that
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Butyrate induces oxidative burst mediated apoptosis via Glucose-6-Phosphate Dehydrogenase (G6PDH) in macrophages during mycobacterial infection Microbes Infect. (IF 5.8) Pub Date : 2023-11-29 Ankush Kumar Rana, Shakuntala Surendra Kumar Saraswati, Vandana Anang, Aayushi Singh, Aarti Singh, Chaitenya Verma, Krishnamurthy Natarajan
Microorganisms present in the gut modulate host defence responses against infections in order to maintain immune homeostasis. This host-microbe crosstalk is regulated by gut metabolites. Butyrate is one such small chain fatty acid produced by gut microbes upon fermentation that has the potential to influence immune responses. Here we investigated the role of butyrate in macrophages during mycobacterial
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Dissecting viral infections, one cell at a time, by single-cell technologies Microbes Infect. (IF 5.8) Pub Date : 2023-11-24 Pierre Bost, Nir Drayman
The meteoric rise of single-cell genomic technologies, especially of single-cell RNA-sequencing (scRNA-seq), has revolutionized several fields of cellular biology, especially immunology, oncology, neuroscience and developmental biology. While the field of virology has been relatively slow to adopt these technological advances, many works have shed new light on the fascinating interactions of viruses
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Real-time, label-free detection and identification of bacteria through non-invasive optical imaging Microbes Infect. (IF 5.8) Pub Date : 2023-11-25 Farah Qazi, Rajni Verma, Connagh E. Redmond, Asma Khalid, Neil M. O'Brien-Simpson, Snjezana Tomljenovic-Hanic
Currently, traditional and newer molecular and mass spectrometry techniques of identifying bacteria from biological samples requires lengthy sample preparation, growth and labelling/staining assays. Thus, there is a pressing clinical need for an adjunct method that accurately identifies bacteria in real time. Here we report on the evaluation of confocal microscopy for the identification of clinically
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Host-pathogen interactions from a metabolic perspective: methods of investigation Microbes Infect. (IF 5.8) Pub Date : 2023-11-23 Caroline Demangel, Laura Surace
Metabolism shapes immune homeostasis in health and disease. This review presents the range of methods that are currently available to investigate the dialog between metabolism and immunity at the systemic, tissue and cellular levels, particularly during infection.
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The influenza A virus promotes fungal growth of Aspergillus fumigatus via direct interaction in vitro Microbes Infect. (IF 5.8) Pub Date : 2023-11-24 Sarah König, Josefine Schroeder, Sandor Nietzsche, Thorsten Heinekamp, Axel A. Brakhage, Roland Zell, Bettina Löffler, Christina Ehrhardt
Seasonal influenza A virus (IAV) infections still pose a major burden for public health worldwide. Severe disease progression or even death is often related to superinfections of the virus and a secondary bacterial pathogen. However, fungi, especially , are also frequently diagnosed during IAV infection. Although, clinical studies have reported the severity of influenza-associated pulmonary aspergillosis
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Chick heterophils release DNA extracellular traps (DETs) in vitro and in vivo upon Aspergillus fumigatus conidia exposure Microbes Infect. (IF 5.8) Pub Date : 2023-11-19 Phillipe de Souza Lima-Gomes, Michelle Tanny Cunha do Nascimento, Natalia Rocha Nadaes, Sérgio Gaspar de Campos, Rosa Maria Tavares Haido, Maria das Graças Danelli, Lucia Helena Pinto-da-Silva, Elvira M. Saraiva
Aspergillosis is a common fungal disease in avian species, causing high mortality in young chicks in agricultural farms and yards. It is caused by fungi belonging to the genus Aspergillosis occurs by inhalation of fungal conidia, and in chickens, effective infection control relies on a rapid and large influx of heterophils to the lungs. Heterophils, upon different stimuli, release to the extracellular
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Use of 2D minilungs from human embryonic stem cells to study the interaction of Cryptococcus neoformans with the respiratory tract Microbes Infect. (IF 5.8) Pub Date : 2023-11-18 Suélen Andreia Rossi, Irene García-Barbazán, Irene Chamorro-Herrero, Carlos Pelleschi Taborda, Óscar Zaragoza, Alberto Zambrano
Organoids can meet the needs between the use of cell culture and work, bringing together aspects of multicellular tissues, providing a more similar system for the study of various components, including host-interactions with pathogens and drug response. Organoids are structures that resemble organs , originating from pluripotent stem cells (PSCs) or adult stem cells (ASCs). There is great interest
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Middle East respiratory coronavirus (MERS-CoV) internalized by llama alveolar macrophages does not result in virus replication or induction of pro-inflammatory cytokines Microbes Infect. (IF 5.8) Pub Date : 2023-11-18 Jordi Rodon, Martin Sachse, Nigeer Te, Joaquim Segalés, Albert Bensaid, Cristina Risco, Júlia Vergara-Alert
Severe Middle East respiratory syndrome (MERS) is characterized by massive infiltration of immune cells in lungs. MERS-coronavirus (MERS-CoV) replicates in human macrophages, inducing high pro-inflammatory responses. In contrast, camelids, the main reservoir for MERS-CoV, are asymptomatic carriers. Although limited infiltration of leukocytes has been observed in the lower respiratory tract of camelids
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Morphogenetic transitions in the adaptation of Candida albicans to the mammalian gut Microbes Infect. (IF 5.8) Pub Date : 2023-11-15 Rebeca Alonso-Monge, Isabel Cortés-Prieto, Elvira Román, Jesús Pla
is a pathobiont in humans that forms part of the mycobiota in healthy individuals and can cause different pathologies upon alterations of the host defenses. The mammalian gut is clinically relevant as this niche is the most common pool for bloodstream-derived infections. The ability of to switch from yeast to hypha has been related to the commensal-to-pathogen transition and is, therefore, considered
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Porphyromonas gingivalis as a promotor in the development of the alcoholic liver disease via ferroptosis Microbes Infect. (IF 5.8) Pub Date : 2023-11-13 Chao Yao, Liyan Lu, Dongmei Lan, Xueqin Zhu, Xue Li, Yaohui Gao, Yingqun Zhou, Yan Wang, Yuanzhi Xu, Shengcai Qi
Alcoholic liver disease (ALD) is a liver disease caused by heavy drinking. (), a major cause of periodontitis, whose antibodies are elevated in severe ALD patients in the plasma. The purpose of this study is to further study the role and the molecular mechanism of in the progress of ALD. In this study, saliva of patients with ALD was collected. Then, an animal model of ALD with oral administration
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A new Salmonella enterica serovar that was isolated from a wild sparrow presents a distinct genetic, metabolic and virulence profile Microbes Infect. (IF 5.8) Pub Date : 2023-11-11 Emiliano Cohen, Shalevet Azriel, Oren Auster, Adiv Gal, Svetlana Mikhlin, Sam Crauwels, Galia Rahav, Ohad Gal-Mor
is a ubiquitous and clinically-important bacterial pathogen, able to infect and cause different diseases in a wide range of hosts. Here, we report the isolation and characterization of a new serovar (13,23:i:-; . Tirat-Zvi), belonging to the Havana supper-lineage that was isolated from a wild house sparrow () in Israel. Whole genome sequencing and complete assembly of its genome indicated a plasmid-free
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SNP-based high-resolution typing of Chlamydia psittaci from humans and wild birds in Sweden: circulation of the Mat116 genotype reveals the transmission mode to humans Microbes Infect. (IF 5.8) Pub Date : 2023-11-11 Björn Herrmann, Rachid Aaziz, Rene Kaden, Hilde M. Riedel, Ellinor Spörndly-Nees, Lisa Labbé Sandelin, Karine Laroucau
The incidence of respiratory tract infections in humans has increased in Sweden in recent years. This study aimed to identify the transmission route by genotyping from infected humans and birds.
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Associations between host microbiome and inflammation suggest role for host microbiome in driving COVID-19 disease severity Microbes Infect. (IF 5.8) Pub Date : 2023-11-08 Rachel MacCann, Tarini Shankar Ghosh, Alejandro Abner Garcia Leon, Junhui Li, Riya Negi, Colette Gaillard, Gurvin Saini, Willard Tinago, Eoin R. Feeney, Obada Yousif, Aoife G. Cotter, Eoghan de Barra, Corinna Sadlier, Peter Doran, Alan Landay, Paul W. O'Toole, Patrick W. Mallon, All Ireland Infectious Diseases cohort study
Systemic inflammation and innate immune activation are associated with COVID-19 disease severity. Knowledge gaps remain in the relationships between microbiome, inflammation and COVID-19 disease severity. To better characterise these associations, we performed 16SrDNA analysis of stool samples in COVID-19 subjects to explore diversity and taxanomic composition. We correlated these to host inflammatory
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Delineating the functional role of the PPE50 (Rv3135) - PPE51 (Rv3136) gene cluster in the pathophysiology of Mycobacterium tuberculosis Microbes Infect. (IF 5.8) Pub Date : 2023-11-04 Ravi Prasad Mukku, Kokavalla Poornima, Sangya Yadav, Tirumalai R. Raghunand
The extraordinary success of () has been attributed to its ability to modulate host immune responses, and its genome encodes multiple immunomodulatory factors, including several proteins of the multigenic PE_PPE family. To understand its role in pathophysiology we have characterised the - gene cluster, one of nine clusters in the genome. We demonstrate here that this cluster is operonic, and that PPE50
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The role of CEACAMs versus integrins in Helicobacter pylori CagA translocation: a systematic review Microbes Infect. (IF 5.8) Pub Date : 2023-11-04 Samaneh Saberi, Nazanin Shans, Saba Ghaffari, Maryam Esmaeili, Marjan Mohammadi
The delivery of Helicobacter pylori CagA into host cells was long believed to occur through the integrin cell surface receptors. However, the role of CEACAM receptors has recently been highlighted, instead. Here, we have categorized the existing experimental evidence according to whether deletion, upregulation, downregulation, or inhibition of the target ligands (T4SS or HopQ) or receptors (integrins
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Insight into the structural hierarchy of the protease cascade that regulates the mosquito melanization response Microbes Infect. (IF 5.8) Pub Date : 2023-11-01 Sally A. Saab, Xiufeng Zhang, Suheir Zeineddine, Bianca Morejon, Kristin Michel, Mike A. Osta
Serine protease cascades regulate important insect immune responses, including melanization and Toll pathway activation. In the context of melanization, central components of these cascades are clip domain serine proteases (CLIPs) including the catalytic, clip domain serine proteases (cSPs) and their non-catalytic homologs (cSPHs). Here, we define partially the structural hierarchy of An. gambiae cSPs
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Corydalis saxicola Bunting total alkaloid eliminates Porphyromonas gingivalis strain 33277 internalized into macrophages by inhibition of TLR2 Microbes Infect. (IF 5.8) Pub Date : 2023-10-31 Lan Yang, Guo-Cheng Mei, Jia-Xuan Wu, Qiao-Zhi Jiang, Zhi-Heng Su, Hai-Qing Liao, Zhen-Min Liu, Ren-Chuan Tao, Xiang-Zhi Yong
Objective This study aimed to investigate the impact of Corydalis Saxicola Bunting Total Alkaloid (CSBTA) on Porphyromonas gingivalis internalization within macrophages and explore the potential role of Toll-Like Receptor 2 (TLR2) in this process. Methods We established a P. gingivalis internalization model in macrophages by treating P. gingivalis-infected macrophages (MOI=100:1) with 200 μg/mL metronidazole
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Human cytokeratin 1 plays a role in the interaction of Pteropine orthoreovirus with Hek293 cells but not HeLa cells Microbes Infect. (IF 5.8) Pub Date : 2023-10-30 Zhen Yun Siew, Yeh Fong Tan, Reuben Parama Iswara, Shew Fung Wong, Siew Tung Wong, Boon Keat Tan, Pooi Pooi Leong, Chee Wah Tan, Lin-Fa Wang, Chee Onn Leong, Kenny Voon
Pteropine orthoreovirus (PRV) causes respiratory tract infections in humans. Despite its emergence as a zoonotic and respiratory virus, little is known about its cell tropism, which hampers progress in fully understanding its pathogenesis in humans. Hek293 cells are most susceptible to PRV infection, while HeLa cells are the least. Human cytokeratin 1 (CK1) was identified as the protein that interacts
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Borrelia burgdorferi colonizes the mammary glands of lactating C3H mice: does not cause congenital Lyme disease Microbes Infect. (IF 5.8) Pub Date : 2023-10-30 Clara Vásquez Velásquez, Mohamed A.M. Moustafa, Sandra C. Rocha, Nikhat Parveen
Transplacental transmission of syphilis causing spirochete, Treponema pallidum subspecies pallidum, from mother to child results in congenital syphilis, an ever-expanding devastating disease worldwide. Although adverse effects of untreated gestational Lyme disease, caused by a related spirochete, Borrelia burgdorferi on fetus viability and development have been observed, cases of congenital Lyme disease