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Echinococcus multilocularis in Red Foxes in Turkey: Increasing risk in urban Acta Trop. (IF 2.555) Pub Date : 2021-01-07 Hamza Avcioglu; Esin Guven; Ibrahim Balkaya; Ridvan Kirman; Muzaffer Akyuz; Mohammed Mebarek Bia; Hatice Gulbeyen; Sali Yaya
This study was conducted to determine the occurrence of E. multilocularis in foxes and environmental fecal contamination by E. multilocularis in Erzurum, the most highly endemic region for AE in Turkey. The study materials consisted of 50 red fox carcasses collected from 20 counties of Erzurum, Turkey, between October 2015 and February 2016. After the application of the sedimentation and counting technique
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Rabies in Tunisia: A spatio-temporal analysis in the region of CapBon-Nabeul Acta Trop. (IF 2.555) Pub Date : 2021-01-07 T. Ben Hassine; M. Ben Ali; I. Ghodhbane; Z. Ben Said; S. Hammami
Human rabies is a significant public health concern in Tunisia. However, the spatiotemporal spread pattern of rabies in dogs, the major reservoir and vector, and its determinants are poorly understood. We collected geographic locations and timeline of reported animal rabies cases in the region of CapBon (study area), for the years 2015–2019 and integrated them in Geographical Information System (GIS)
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Improved Aedes/dengue field surveillance using Gravid Oviposition Sticky trap and dengue NS1 tests: epidemiological, entomological outcomes and community acceptance Acta Trop. (IF 2.555) Pub Date : 2021-01-16 Jonathan Wee Kent Liew; Sivaneswari Selvarajoo; Wei Kit Phang; Mukhainizam Mah Hassan; Mohd Sabri Redzuan; Shurendar Selva Kumar; Jeremy Ryan de Silva; Yee Ling Lau; Indra Vythilingam
The aim of this study is to investigate the feasibility and outcomes of using Gravid Oviposition Sticky (GOS) trap and dengue NS1 antigen tests for indoor and outdoor dengue/Aedes surveillance in the field. A one-year community-based study was carried out at Sungai Buloh Hospital Quarters, Selangor, Malaysia. GOS traps were first placed outdoors in three apartment blocks (Anggerik, Bunga Raya and Mawar)
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Derivatisation of metronidazole enhances cytotoxic effect against Acanthamoeba genotype T4 isolates and leads to cytomorphological changes in trophozoites Acta Trop. (IF 2.555) Pub Date : 2021-01-16 Aneta Dušeková; Mária Garajová; Miloš Lukáč; Martin Mrva
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Species diversity and blood meal sources of phlebotomine sand flies (Diptera: Psychodidae) from Los Tuxtlas, Veracruz, Mexico Acta Trop. (IF 2.555) Pub Date : 2021-01-16 Yokomi N Lozano-Sardaneta; Eduardo I. Jiménez-Girón; Jorge J. Rodríguez-Rojas; Sokani Sánchez-Montes; Lucía Álvarez-Castillo; Víctor Sánchez-Cordero; Ingeborg Becker
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Current methods for the detection of antimalarial drug resistance in Plasmodium parasites infecting humans Acta Trop. (IF 2.555) Pub Date : 2021-01-16 Lucinda Slater; Martha Betson; Shoaib Ashraf; Neil Sargison; Umer Chaudhry
Malaria is the world's deadliest parasitic disease. Great progress has been made in the fight against malaria over the past two decades, but this has recently begun to plateau, in part due to the global development of antimalarial drug resistance. The ability to track drug resistance is necessary to achieve progress in treatment, disease surveillance and epidemiology, which has prompted the development
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Urban ecology of hosts and vectors of Rickettsia in a rickettsiosis-endemic city of the Yucatan peninsula, Mexico. Acta Trop. (IF 2.555) Pub Date : 2021-01-16 Karla R. Dzul-Rosado; Enrique Reyes-Novelo; Cesar Lugo-Caballero; Alan D. Cuxim-Koyoc; Francisco Collí-Padrón; Raul Tello-Martín; Karina B. López-Ávila; Adolfo Palma-Chan; Gaspar Peniche-Lara; Hugo A. Ruiz-Piña
Rickettsioses are vector-borne zoonotic diseases that occur in urban environments. Currently, they are associated with the presence of domestic and synanthropic animals, the ectoparasites that they harbor, and their local habitat. The implementation of prevention actions relies on the understanding of the local ecology of interactions between hosts, vector species, and the etiologic agents. In this
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Investigation of Phlebotominae (Diptera: Psychodidae) Fauna, Seasonal Dynamics, and Natural Leishmania spp. Infection in Muğla, Southwest of Turkey Acta Trop. (IF 2.555) Pub Date : 2021-01-09 Metin Pekağırbaş; Mehmet Karakuş; Ozge Erisoz Kasap; Samiye Demir; Muhammed Nalçacı; Seray Töz; Hasan Eren; Yusuf Özbel
Due to its geographical location, Muğla province is one of the most frequently used places by refugees. Although leishmaniasis have been previously reported in this region, there is a lack of information on the etiological agent and possible vectors. The main objectives of this study were; i) to investigate the sand fly fauna, ii) to reveal the natural Leishmania spp. infection in wild caught sand
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Proteomic analysis of hydrolytic proteases in excretory/secretory proteins from Trichinella spiralis intestinal infective larvae using zymography combined with shotgun LC-MS/MS approach Acta Trop. (IF 2.555) Pub Date : 2021-01-07 Hua Nan Ren; Tong Xu Zhuo; Sheng Jie Bai; Ying Bai; Xiang Yuan Sun; Ruo Dan Liu; Shao Rong Long; Jing Cui; Zhong Quan Wang
The critical step of Trichinella spiralis infection is that the muscle larvae (ML) are activated to intestinal infective larvae (IIL) which invade the intestinal columnar epithelium to further develop. The IIL excretory/secretory (ES) proteins play an important role in host-parasite interaction. Proteolytic enzymes are able to mediate the tissue invasion, thereby increasing the susceptibility of parasites
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Performance of pirimiphos-methyl based Indoor Residual Spraying on entomological parameters of malaria transmission in the pyrethroid resistance region of Koulikoro, Mali Acta Trop. (IF 2.555) Pub Date : 2021-01-02 Moussa Keïta; Nafomon Sogoba; Boïssé Traoré; Fousseyni Kané; Boubacar Coulibaly; Sekou Fantamady Traoré; Seydou Doumbia
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Phylogenetic analysis suggests single and multiple origins of dihydrofolate reductase mutations in Plasmodium vivax Acta Trop. (IF 2.555) Pub Date : 2021-01-03 Ayaz Shaukat; Qasim Ali; Lucy Raud; Abdul Wahab; Taj Ali Khan; Imran Rashid; Muhammad Rashid; Mubashir Hussain; Mushtaq A. Saleem; Neil D. Sargison; Umer Chaudhry
Pyrimethamine was first introduced for the treatment of malaria in Asia and Africa during the early 1980s, replacing chloroquine, and has become the first line of drugs in many countries. In recent years, development of pyrimethamine resistance in Plasmodium vivax has become a barrier to effective malaria control strategies. Here, we describe the use of meta-barcoded deep amplicon sequencing technology
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Host snail species exhibit differential Angiostrongylus cantonensis prevalence and infection intensity across an environmental gradient Acta Trop. (IF 2.555) Pub Date : 2021-01-07 Randi L. Rollins; Robert H. Cowie; Ma Vida Echaluse; Matthew C.I. Medeiros
Diverse snail species serve as intermediate hosts of the parasitic nematode Angiostrongylus cantonensis, the etiological agent of human neuroangiostrongyliasis. However, levels of A. cantonensis infection prevalence and intensity vary dramatically among these host species. Factors contributing to this variation are largely unknown. Environmental factors, such as precipitation and temperature, have
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Role of cytokines produced by T helper immune-modulators in dengue pathogenesis: a systematic review and meta-analysis Acta Trop. (IF 2.555) Pub Date : 2021-01-07 Linh Tran; Ibrahim Radwan; Le Huu Nhat Minh; Soon Khai Low; Mohammad Rashidul Hashan; Mohammad Diaa Gomaa; Mohamed Abdelmongy; Abdullah Abdel Aziz; Alaa Mohamed; Gehad Mohamed Tawfik; Shusaku Mizukami; Kenji Hirayama; Nguyen Tien Huy
Background and objectives : Modulation of the immune reaction is essential in the development of various diseases, including dengue's “Cytokine Tsunami”, an increase in vascular permeability with concomitant severe vascular leakage. We aim to identify the role of T-helper (Th) cells, Th2 and Th7, with their related cytokines in dengue pathogenesis. Material and methods : Nine electronic databases and
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Applications of artificial membrane feeding for ixodid ticks Acta Trop. (IF 2.555) Pub Date : 2021-01-03 Julia González; Mathew Bickerton; Alvaro Toledo
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Use of Immunomagnetic Separation tool in Leishmania promastigotes capture Acta Trop. (IF 2.555) Pub Date : 2020-12-29 Imen Tayachi; Yousr Galai; Meriem Ben-abid; Nasreddine Saidi; Ines Ben-sghaier; Karim Aoun; Aïda Bouratbine
Immunomagnetic Separation (IMS) assay has been used for isolation of viable whole organisms. The objective of our work is to produce anti-Leishmania magnetic beads and to assess the efficiency of the IMS technique on Leishmania promastigote capture in culture media. Polyclonal anti-Leishmania antibodies were produced by intravenous injection of viable metacyclic promastigotes of Leishmania (L.) major
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Orientia tsutsugamushi DNA load and genotypes in blood as a marker of severity Acta Trop. (IF 2.555) Pub Date : 2020-12-09 Seok Won Kim; Choon-Mee Kim; Dong-Min Kim; Na Ra Yun; Ganesh P. Neupane; Sang-Hyun Pyun; Byeong Jeon Yu
Scrub typhus is an acute febrile illness caused by Orientia tsutsugamushi and is endemic to many parts of the Asia-Pacific region. We investigated whether the genotype of O. tsutsugamushi or the DNA load would be a useful marker of disease severity in scrub typhus patients. We evaluated the clinical features, genotypes and bacterial DNA load in the blood of 118 patients, including 114 surviving and
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First molecular identification of Leishmania major in Phlebotomus papatasi in an outbreak cutaneous leishmaniasis area in Iraq Acta Trop. (IF 2.555) Pub Date : 2020-12-29 Mariwan M.M. Al-Bajalan; Sherko S. Niranji; Sirwan M. Al-Jaf; Hirotomo Kato
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Simultaneous circulation of Zika, Dengue, and Chikungunya viruses and their vertical co-transmission among Aedes aegypti Acta Trop. (IF 2.555) Pub Date : 2021-01-03 Adriano Fernandes Teixeira; Breno Bittencourt de Brito; Thiago Macêdo Lopes Correia; Airton Idalécio Sousa Viana; Juliana Cabral Carvalho; Filipe Antônio França da Silva; Maria Luísa Cordeiro Santos; Eliezer Almeida da Silveira; Henry Paul Granger Neto; Natalia Maria Pereira da Silva; Cínthya Viana Souza Rocha; Fillipe Dantas Pinheiro; Bárbara Aparecida Chaves; Wuelton Marcelo Monteiro; Marcus Vinícius
The outbreaks caused by the Aedes aegypti-transmitted dengue virus (DENV), zika virus (ZIKV), and chikungunya virus (CHIKV) result in a significant impact to the health systems of tropical countries. Furthermore, the occurrence of patients coinfected by at least two of these arboviruses is an aggravating factor in that scenario. On this basis, surveillance tools such as the Rapid Index Survey for Aedes
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Modeling dengue vector population with earth observation data and a generalized linear model Acta Trop. (IF 2.555) Pub Date : 2020-12-29 Oladimeji Mudele; Alejandro C. Frery; Lucas F.R. Zanandrez; Alvaro E. Eiras; Paolo Gamba
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The risk of oral transmission in an area of a Chagas disease outbreak in the Brazilian northeast evaluated through entomological, socioeconomic and schooling indicators Acta Trop. (IF 2.555) Pub Date : 2020-12-26 Jackeline Monsalve-Lara; Maurício Lilioso; Carolina Valença-Barbosa; Patricia J Thyssen; Danilo C Miguel; Cleanne Limeira; Fernanda R Gadelha; Fernanda V H M Fontes; Dayane Pires-Silva; L Lynnette Dornak; Marli M Lima; Maria R Donalisio; Carlos E Almeida
Chagas disease is a neglected tropical disease strongly associated with low socioeconomic status, affecting nearly 8 million people – mainly Latin Americans. The current infection risk is based on acute case reports, most of which are typically associated with oral transmissions. In the semi-arid region of Northeastern Brazil, serious outbreaks of this transmission type have surged in the last years
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INFLUENCE OF GAMMA RADIATION ON AMPHOTERICIN B INCORPORATED IN PVP HYDROGEL AS AN ALTERNATIVE TREATMENT FOR CUTANEOUS LEISHMANIOSIS Acta Trop. (IF 2.555) Pub Date : 2020-12-30 Maria José Alves de Oliveira; Gethzemani Mayeli Estrada Villegas; Flávia Daniela Motta; Omar Fabela-Sánchez; Arián Espinosa-Roa; Wesley Luzetti Fotoran; Janaína Capelli Peixoto; Fabia Tomie Tano; Ademar Benévolo Lugão; Pablo Antonio Salvador Vásquez
Amphotericin B (Amph-B) is an antifungal drug used intravenously for the treatment of leishmaniasis. Side-effects from Amph-B treatment can arise such as cardiac arrhythmia and renal dysfunctions, which will lead to discontinuation of treatment. Unfortunately, patients in endemic countries do not have access to alternative therapies. The objective of this study was to analyze the effects of Cobalt-60
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Use of Schistosoma mansoni soluble egg antigen (SEA) for antibody detection and diagnosis of schistosomiasis: The need for improved accuracy evaluations of diagnostic tools Acta Trop. (IF 2.555) Pub Date : 2020-12-23 Carlos Graeff-Teixeira; Vivian Favero; Renata Perotto de Souza; Vanessa Fey Pascoal; Hélio Radke Bittencourt; Mizuho Fukushige; Stefan Michael Geiger; Deborah Negrão-Corrêa
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Molecular species delimitation reveals high diversity in the mosquito Anopheles tessellatus Theobald, 1901 (Diptera, Culicidae) across its range Acta Trop. (IF 2.555) Pub Date : 2020-12-23 Brian P. Bourke; Richard C. Wilkerson; Yvonne-Marie Linton
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Susceptibility to paromomycin in clinical isolates and reference strains of Leishmania species responsible for tegumentary leishmaniasis in Brazil Acta Trop. (IF 2.555) Pub Date : 2020-12-29 Elizabeth M. Coser; Bianca A. Ferreira; Edite H. Yamashiro-Kanashiro; José Angelo L. Lindoso; Adriano C. Coelho
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Rabies control in Liberia: Joint efforts towards Zero by 30 Acta Trop. (IF 2.555) Pub Date : 2020-12-29 Garmie Voupawoe; Roland Varkpeh; Varney Kamara; Sonpon Sieh; Abdallah Traoré; Cristian De Battisti; Angélique Angot; Luis Filipe L de J Loureiro; Baba Soumaré; Gwenaëlle Dauphin; Wolde Abebe; André Coetzer; Terence Scott; Louis Nel; Jesse Blanton; Laurent Dacheux; Simon Bonas; Hervé Bourhy; Stephanie Mauti
Despite declaration as a national priority disease, dog rabies remains endemic in Liberia, with surveillance systems and disease control activities still developing. The objective of these initial efforts was to establish animal rabies diagnostics, foster collaboration between all rabies control stakeholders, and develop a short-term action plan with estimated costs for rabies control and elimination
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Increasing rabies data availability: The example of a One Health research project in Chad, Côte d'Ivoire and Mali Acta Trop. (IF 2.555) Pub Date : 2020-12-29 Monique Léchenne; Abdallah Traore; Jan Hattendorf; Vessaly Kallo; Assandi Oussiguere; Mathilde Tetchi; Ronelngar Moyengar; Abdel Kader Traore; Daugla Doumagoum Moto; Richard Ngandolo; Bassirou Bonfoh; Jakob Zinsstag
Rabies is a fatal but preventable disease that remains notoriously underreported. Weak data availability hampers advocacy, constitutes a barrier to resource allocation and inhibits effective prevention and control. To gain better insight into the global rabies burden and human vaccine demand several studies were funded through the Vaccine Alliance (GAVI) learning agenda. With the help of this funding
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3,5-Dimethyl-4-isoxazoyl selenocyanate as promising agent for the treatment of Leishmania infantum-infected mice Acta Trop. (IF 2.555) Pub Date : 2020-12-23 Verónica Alcolea; Esther Moreno; Mikel Etxebeste-Mitxeltorena; Iñigo Navarro-Blasco; Elena González-Peñas; Antonio Jiménez-Ruiz; Juan Manuel Irache; Carmen Sanmartín; Socorro Espuelas
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Tick-borne rickettsiae in Midwestern region of Republic of Korea Acta Trop. (IF 2.555) Pub Date : 2020-12-10 Hye-Jin Park; Jeoungyeon Kim; Yeon-Joo Choi; Heung-Chul Kim; Terry A Klein; Sung-Tae Chong; Ju Jiang; Allen L. Richards; Won-Jong Jang
To identify spotted fever group (SFG) rickettsiae among ticks collected by dragging at eight sites in three provinces of the midwestern region of the Republic of Korea (ROK), genus- and species-specific quantitative real-time PCR (qPCR) assays and sequencing were performed. DNA was extracted from a total of 2,312 ticks that were assayed individually (n=140) or in pools (n=444), resulting in a total
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Epidemiology of malaria among HIV/AIDS patients in sub-Saharan Africa: A systematic review and meta-analysis of observational studies Acta Trop. (IF 2.555) Pub Date : 2020-12-17 Oluwasola Olaiya Obebe; Olufarati Oludunsin Falohun
Malaria related HIV morbidity and death is a concern in sub-Saharan Africa. Understanding the epidemiology of malaria among people living with HIV is vital for adequate intervention. We conducted a systematic review and meta-analysis to estimate the prevalence of malaria in HIV patients in sub-Saharan Africa. We searched PubMed, AJOL, Web of Science and Google Scholar databases. The overall pooled
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A Bayesian Functional Methodology for Dengue Risk Mapping in Latin America and the Caribbean Acta Trop. (IF 2.555) Pub Date : 2020-12-15 A Torres-Signes; J.A. Dip
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The pests of a pest: A systematic review of ectoparasitic fauna among synanthropic rodents in the 21st century with meta-analysis Acta Trop. (IF 2.555) Pub Date : 2020-12-23 Jeffery Ho; Tanasak Changbunjong; Thekhawet Weluwanarak; Sabir Hussain; Olivier Sparagano
Synanthropic rodents are important urban pests that frequently carry hematophagous ectoparasites. These blood-sucking pests are capable of transmitting epizootic and zoonotic pathogens by landing on one host after feeding on an infected animal. This study aimed to estimate the prevalence of ectoparasites carried by synanthropic rodents and discuss the pathogens that are associated with these external
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Spring temperature shapes West Nile virus transmission in Europe Acta Trop. (IF 2.555) Pub Date : 2020-12-10 Giovanni Marini; Mattia Manica; Luca Delucchi; Andrea Pugliese; Roberto Rosà
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Misdiagnosis of leprosy in Brazil in the period 2003 - 2017: spatial pattern and associated factors Acta Trop. (IF 2.555) Pub Date : 2020-12-11 Karine Vila Real Nunes Neves; Maurício Lisboa Nobre; Lúbia Maieles Gomes Machado; Peter Steinmann; Eliane Ignotti
Background Leprosy causes a range of symptoms, and most diagnoses are established based on the clinical picture. Therefore, false negative and positive diagnoses are relatively common. We analyzed the spatial pattern of leprosy misdiagnosis and associated factors in Brazil. Method Exploratory analyses of Kernel density of the new case detection rate (NCDR) and proportion of misdiagnosis in Brazil,
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Green-synthesized metal nanoparticles for mosquito control: A systematic review about their toxicity on non-target organisms Acta Trop. (IF 2.555) Pub Date : 2020-12-11 Loick P. Kojom Foko; Francois Eya'ane Meva; Carole E. Eboumbou Moukoko; Agnes A. Ntoumba; Wolfgang E. Ekoko; Philippe Ebanda Kedi Belle; Gildas P. Ndjouondo; Godlove W. Bunda; Leopold G. Lehman
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An Ixodes scapularis glutathione S-transferase plays a role in cell survival and viability during Langat virus infection of a tick cell line Acta Trop. (IF 2.555) Pub Date : 2020-11-24 Emmanuel Pacia Hernandez; Melbourne Rio Talactac; Rodel Jonathan Santos Vitor; Kentaro Yoshii; Tetsuya Tanaka
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Childhood visceral leishmaniasis in Tunisia: A cross-sectional study in local spatial analysis Acta Trop. (IF 2.555) Pub Date : 2020-12-10 Kais Ben-Ahmed
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Ngari virus (Orthobunyavirus, Peribunyaviridae) in ixodid ticks collected from cattle in Guinea Acta Trop. (IF 2.555) Pub Date : 2020-12-09 Marat T. Makenov; Aboubacar H. Toure; Roman B. Bayandin; Anastasia V. Gladysheva; Andrey V. Shipovalov; Sanaba Boumbaly; Noumouny Sacko; Mikhail G. Korneev; Sergey A. Yakovlev; Olga B. Zhurenkova; Yana E. Grigoreva; Marina V. Fyodorova; Ekaterina V. Radyuk; Evgeny S. Morozkin; Mamadou Y. Boiro; Alina Matsvay; Kamil Khafizov; Lyudmila S. Karan
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The prevalence of cercarial infection and development of a duplex PCR for detection of the cercarial stage of Haplorchis taichui and H. pumilio in first intermediate hosts from Chai Nat province, Thailand Acta Trop. (IF 2.555) Pub Date : 2020-12-10 Chadaporn Dunghungzin; Thapana Chontananarth
This study aimed to investigate the prevalence of cercarial infections in freshwater snails collected from the Chai Nat province of Central Thailand. Moreover, we aimed to develop a duplex PCR technique, using the ITS2 and candidate MT-ND1 gene, to determine the dissemination of H. taichui and H. pumilio, respectively. Six types of cercariae were discovered with an overall prevalence of 4.71% (59/1
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Effects of a solid formulation containing lectin-rich fraction of Moringa oleifera seeds on egg hatching and development of Aedes aegypti larvae Acta Trop. (IF 2.555) Pub Date : 2020-12-09 Livia Lais de Santana Silva; Stella Cristina Cabral Silva; Ana Patrícia Silva de Oliveira; Jéssica da Silva Nascimento; Emerson de Oliveira Silva; Luana Cassandra Breitenbach Barroso Coelho; Pedro José Rolim Neto; Daniela Maria do Amaral Ferraz Navarro; Thiago Henrique Napoleão; Patrícia Maria Guedes Paiva
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Vector competence of Culex tritaeniorhynchus and Culex quinquefasciatus (Diptera: Culicidae) for duck Tembusu virus transmission Acta Trop. (IF 2.555) Pub Date : 2020-12-09 Jitra Sanisuriwong; Nichapat Yurayart; Aunyaratana Thontiravong; Sonthaya Tiawsirisup
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Systematic review and meta-analysis of diagnostic tests for diagnosis of melioidosis Acta Trop. (IF 2.555) Pub Date : 2020-12-06 Lachlan Fairley; Samuel Smith; Shalisa Maisrikrod; Lars Henning
Objectives Melioidosis is an emerging tropical disease caused by B. pseudomallei that can rapidly prove fatal and require prompt and appropriate antibiotic treatment. Diagnosis currently relies on culture; however, this delays appropriate antibiotics and contributes to mortality as results can take up to one week or more. Several non-culture based diagnostic tests are available; however, their role
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One year update on the COVID-19 pandemic: Where are we now? Acta Trop. (IF 2.555) Pub Date : 2020-11-28 Sanjay Kumar Mishra; Timir Tripathi
We are living through an unprecedented crisis with the rapid spread of the new coronavirus disease (COVID-19) worldwide within a short time. The timely availability of thousands of SARS-CoV-2 genomes has enabled the scientific community to study the origin, structures, and pathogenesis of the virus. The pandemic has spurred research publication and resulted in an unprecedented number of therapeutic
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Artesunate alleviates schistosomiasis-induced liver fibrosis by downregulation of mitochondrial complex Ⅰ subunit NDUFB8 and complex Ⅲ subunit UQCRC2 in hepatic stellate cells Acta Trop. (IF 2.555) Pub Date : 2020-11-29 Shuang Shen; Juntao Luo; Jianping Ye
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Clinical and biochemical parameters of COVID-19 patients with prior or active dengue fever Acta Trop. (IF 2.555) Pub Date : 2020-11-28 Isabella Márcia Soares Nogueira Teotônio; Juliana Lott de Carvalho; Luiz Cláudio Castro; Nadjar Nitz; Luciana Hagström; Geraldo Gonçalves Rios; Maria de Fátima Rodrigues de Oliveira; Bruno Stéfano Lima Dallago; Mariana Hecht
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Molecular prevalence, risk factors and genotypes of Toxoplasma gondii DNA in wild marine snails collected from offshore waters in eastern China Acta Trop. (IF 2.555) Pub Date : 2020-11-28 Wei Cong; Hany M. Elsheikha; Man-Yao Li; Jun-Yang Ma; Yang Zou; Zhao-Yang Jiang
Increasing evidence exisits for the role that shellfish play in the epidemiology of Toxoplasma gondii in marine environment. However, limited information is available on the level of T. gondii infection in wild marine snails, which can play a role in the transmission of T. gondii to other marine organisms and humans. In this study, the prevalence of T. gondii DNA in wild marine snails collected from
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Tackling zoonoses in a crowded world: Lessons to be learned from the COVID-19 pandemic Acta Trop. (IF 2.555) Pub Date : 2020-11-28 Alex Córdoba-Aguilar; Carlos N. Ibarra-Cerdeña; Iván Castro-Arellano; Gerardo Suzan
The COVID-19 zoonosis is bringing about a number of lessons to humanity. One is that of transforming our links with nature and, particularly, wildlife given the likely COVID-19 origin from illegal wildlife trading. Similar to vector borne diseases (VBD, diseases transmitted by vectors), the COVID-19 pandemic follows related patterns (e.g. no effective or available vaccines, difficult to diagnose, highly
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Description of the ovarian microbiota of Aedes aegypti (L) Rockefeller strain Acta Trop. (IF 2.555) Pub Date : 2020-11-24 Wilber A. Alvarado; Susana Ochoa Agudelo; Iván Darío Velez; Rafael José. Vivero
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Ex vivo infection of canine and ovine placental explants with Trypanosoma cruzi and Toxoplasma gondii: differential activation of NF kappa B signaling pathways Acta Trop. (IF 2.555) Pub Date : 2020-11-24 Ana Liempi; Christian Castillo; Lisvaneth Medina; Maura Rojas-Pirela; Sebastian Araneda; Juan Diego Maya; Victor H. Parraguez; Ulrike Kemmerling
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Bioenergetics impairment of Trypanosoma cruzi by the antihypertensive manidipine: A drug repurposing strategy Acta Trop. (IF 2.555) Pub Date : 2020-11-25 Isabela Teresa Santos Correa; Thais Alves da Costa-Silva; Andre Gustavo Tempone
Considering the lack of effective and safe therapy for the treatment of Chagas disease, the antihypertensive drug manidipine (MDP) was in vitro evaluated against Trypanosoma cruzi. The bioenergetics of trypomastigotes was studied in the presence of the drug using fluorimetric and luminescent assays. Manidipine showed a potent antiparasitic activity, with IC50 values of 0.1 μM (intracellular amastigotes)
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Intestinal parasites in segregated minority communities of Slovakia: results from a cross-sectional survey in children Acta Trop. (IF 2.555) Pub Date : 2020-11-28 Ingrid Papajovà; Julia Bystrianska; Michal Giboda; Sören L. Becker; Jürg Utzinger; Hanspeter Marti
In Slovakia, the Roma population forms the second-largest ethnic minority. A large part of the Roma is semi-nomadic or lives in segregated settlements with poor living standards and limited access to health facilities. More than 40 years ago, a cross-sectional survey revealed a high prevalence of parasitic infections. There is a paucity of recent data, and hence, we designed a study in order to investigate
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Modified DMEM xenic culture medium for propagation, isolation and maintenance of Balantioides coli Acta Trop. (IF 2.555) Pub Date : 2020-11-21 Wenchao Yan; Tianqi Wang; Lizhuo Zhao; Chenyang Sun
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Recombinant Expression And Indirect ELISA For COWP And HSP70 Proteins From Cryptosporidium andersoni Acta Trop. (IF 2.555) Pub Date : 2020-11-24 Hao Peng; Linsheng Tang; Cuilan Wu; Jun Li; Li Tao; Changting Li; Zhongwei Chen; Yongping Xie
Cryptosporidium spp. infect cattle at a high rates, and reduce milk production. Cryptosporidiosis has caused economic losses for the dairy industry. Studies in Western countries have shown that Cryptosporidium can also infect humans. Therefore, the development of methods for the early detection of Cryptosporidium is an important public health objective. Total RNA isolated from C. andersoni was used
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Modeling host-feeding preference and molecular systematics of mosquitoes in different ecological niches in Canada Acta Trop. (IF 2.555) Pub Date : 2020-11-04 Nariman Shahhosseini; Christina Frederick; Trina Racine; Gary P. Kobinger; Gary Wong
Several mosquito-borne viruses (mobovirus) cause infections in Canada. Ecological data on mosquito species and host range in Canada remains elusive. The main aim of the current study is to determine the host range and molecular systematics of mosquito species in Canada. Mosquitoes were collected using BG-Sentinel traps and aspirators at 10 trapping sites in Canada during 2018 and 2019. Mosquitoes collected
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Serological, molecular and bacteriological approaches for detecting Leptospira sp. carrier rams maintained in semiarid conditions Acta Trop. (IF 2.555) Pub Date : 2020-11-13 Rafael Rodrigues Soares; Nathanel Natércio da Costa Barnabé; Denise Batista Nogueira; Laís Samara Cavalcante da Silva; João Pessoa Araújo Júnior; Camila Dantas Malossi; Leila Sabrina Ullmann; Diego Figueiredo da Costa; Maria Luana Cristiny Rodrigues Silva; Severino Silvano dos Santos Higino; Sergio Santos de Azevedo; Clebert José Alves
Even in the adverse environmental conditions of the semiarid region, leptospires can survive and spread by alternative routes of transmission, such as sexual in ewes, however, there is no data on rams. Thus, the present study aimed to evaluate the use of serological, molecular and microbial tools applied to diagnosis of Leptospira sp. Infection in rams maintained in semiarid conditions. Biological
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Modelling the present and future distribution of Biomphalaria species along the watershed of the Middle Paranapanema region, São Paulo, Brazil Acta Trop. (IF 2.555) Pub Date : 2020-11-21 Raquel Gardini Sanches Palasio; Thiago Salomão de Azevedo; Roseli Tuan; Francisco Chiaravalloti-Neto
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Methods for detecting insecticide resistance in sand flies: A systematic review Acta Trop. (IF 2.555) Pub Date : 2020-11-11 Douglas de Almeida Rocha; Lidiane Medeiros da Costa; Grasielle D'Ávila Caldas Pessoa; Marcos Takashi Obara
The classification of insecticide resistance in sand flies populations is based on concepts and methodologies used to characterize the susceptibility profile in mosquitoes. This can generate erroneous and subjective interpretations since they are biologically different organisms. In this context, the goal of this review is to analyze the works and/or articles that aimed at characterizing the susceptibility
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Identification of mosquitoes (Diptera: Culicidae) from Mexico State, Mexico using morphology and COI DNA barcoding Acta Trop. (IF 2.555) Pub Date : 2020-10-21 Adebiyi A. Adeniran; Luis M. Hernández-Triana; Aldo I. Ortega-Morales; Javier A. Garza-Hernández; Josué de la Cruz-Ramos; Rahuel J. Chan-Chable; Rafael Vázquez-Marroquín; Herón Huerta-Jiménez; Nadya I. Nikolova; Anthony R. Fooks; Mario A. Rodríguez-Pérez
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A cross-sectional survey on parasitic infections in schoolchildren in a rural Tanzanian community Acta Trop. (IF 2.555) Pub Date : 2020-11-04 Marta S. Palmeirim; Emmanuel C. Mrimi; Elihaika G. Minja; Aziza J. Samson; Jennifer Keiser
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Congenital chagas disease: Development and assessment of a specific IgM capture-based assay for diagnosis of transmission Acta Trop. (IF 2.555) Pub Date : 2020-11-04 Luz María Peverengo; Luz María Rodeles; Camila Maldonado; Griselda Ballering; Nazarena Pujato; Indira D’Amico; Miguel Hernán Vicco; Luciana Garcia; Laura Jurado; Jaime Altcheh; Iván Marcipar
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Comparative analysis of different extracellular vesicles secreted by Echinococcus granulosus protoscoleces Acta Trop. (IF 2.555) Pub Date : 2020-11-13 Jin'en Wu; Mengting Cai; Jing Yang; Yating Li; Juntao Ding; Omnia M. Kandil; Ivan Kutyrev; Mazhar Ayaz; Yadong Zheng
Extracellular vesicles (EVs) are heterogeneous populations of different membrane-wrapped vesicles in size and encapsulated cargo and have recently emerged as a crucial carrier with the functions in intercellular communication, being involved in host-parasite interactions. However, Echinococcus granulosus EVs are not fully described. To separate EVs with a different size, the culture supernatant of