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Regulation mechanism of EBV-encoded EBER1 and LMP2A on YAP1 and the impact of YAP1 on the EBV infection status in EBV-associated gastric carcinoma Virus Res. (IF 5.0) Pub Date : 2024-03-11 Yujie Sun, Duo Shi, Jiting Sun, Yan Zhang, Wen Liu, Bing Luo
This study aims to explore the role and regulatory mechanism of Yes-associated protein 1 (YAP1) in the development of Epstein-Barr virus-associated gastric cancer (EBVaGC). Here we showed that EBV can upregulate the expression and activity of YAP1 protein through its encoded latent products EBV-encoded small RNA 1 (EBER1) and latent membrane protein 2A (LMP2A), enhancing the malignant characteristics
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Molecular and biological characterization of a novel partitivirus from Talaromyces pinophilus Virus Res. (IF 5.0) Pub Date : 2024-03-11 Sidra Hassan, Urayama Syun-ichi, Saba Shabeer, Tahseen Ali Kiran, Chien-Fu Wu, Hiromitsu Moriyama, Robert H.A. Coutts, Ioly Kotta Loizou, Atif Jamal
spp. have a worldwide distribution, are ecologically diverse and have been isolated from numerous different substrates. spp. are considered biotechnologically important due to their ability to produce a range of enzymes and pigments. , belonging to genus and family , is known for producing several important bioactive metabolites. Here we report the isolation and characterisation of a partitivirus from
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Do bed bugs transmit human viruses, or do humans spread bed bugs and their viruses? A worldwide survey of the bed bug RNA virosphere Virus Res. (IF 5.0) Pub Date : 2024-03-07 Hunter K. Walt, Jonas G. King, Johnathan M. Sheele, Florencia Meyer, Jose E. Pietri, Federico G. Hoffmann
(Hemiptera: Cimicidae) are a globally distributed hematophagous pest that routinely feed on humans. Unlike many blood-sucking arthropods, they have never been linked to pathogen transmission in a natural setting, and despite increasing interest in their role as disease vectors, little is known about the viruses that bed bugs naturally harbor. Here, we present a global-scale survey of the bed bug RNA
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Interleukin-1β promotes human metapneumovirus replication via activating the cGAS-STING pathway Virus Res. (IF 5.0) Pub Date : 2024-03-04 Guojin Wu, Yueyan Zhang, Linlin Niu, Yuan Hu, Yuting Yang, Yao Zhao
Human metapneumovirus(hMPV) is one of the most common viruses that cause acute lower respiratory tract infections. Interleukin-1β (IL-1β) has been reported to play an important role in multiple virus replication. Patients with hMPV infection have increased levels of IL-1β which reminds IL-1β is associated with hMPV infection. However, the mechanism by which IL-1β affects hMPV replication remains unclear
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Role of long non-coding RNA DLY6E in regulating TMUV infection Virus Res. (IF 5.0) Pub Date : 2024-03-04 Siming Zhu, Xin Chen, Dalin He, Meijuan Zhang, Xinhong Man, Yi Tang, Youxiang Diao
Long non-coding RNA (lncRNA) is a type of RNA with a length greater than 200 nt and lacking coding ability. In recent years, a considerable number of lncRNAs have been found to have important functions. The lncRNA plays an important role in growth and development, body metabolism, immune function, and regulation of viral replication. A lncRNA, MSTRG8505.2, was screened and named lncRNA DLY6E, which
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Deletion of the B125R gene in the African swine fever virus SY18 strain leads to an A104R frameshift mutation slightly attenuating virulence in domestic pigs Virus Res. (IF 5.0) Pub Date : 2024-03-04 Rongnian Zhu, Ying Wang, Han Zhang, Jinjin Yang, Jiaqi Fan, Yanyan Zhang, Yu Wang, Qixuan Li, Xintao Zhou, Huixian Yue, Yu Qi, Shuchao Wang, Teng Chen, Shoufeng Zhang, Rongliang Hu
African swine fever (ASF), caused by the ASF virus (ASFV), is a hemorrhagic and fatal viral disease that affects Eurasian wild boars and domestic pigs, posing a substantial threat to the global pig breeding industry. ASFV, a double-stranded DNA virus, possesses a large genome containing up to 160 open reading frames, most of which exhibit unknown functions. The B125R gene of ASFV, located at the 105595–105972
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African swine fever virus early protein pI73R suppresses the type-I IFN promoter activities Virus Res. (IF 5.0) Pub Date : 2024-03-01 Danh Cong Lai, Jayeshbhai Chaudhari, Hiep L.X. Vu
African swine fever virus is known to suppress type-I interferon (IFN) responses. The main objective of this study was to screen early-expressed viral genes for their ability to suppress IFN production. Out of 16 early genes examined, I73R exhibited robust suppression of cGAS-STING-induced IFN-β promoter activities, impeding the function of both IRF3 and NF-κB transcription factors. As a result, I73R
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Exploring COVID-19 causal genes through disease-specific Cis-eQTLs Virus Res. (IF 5.0) Pub Date : 2024-02-25 Sainan Zhang, Ping Wang, Lei Shi, Chao Wang, Zijun Zhu, Changlu Qi, Yubin Xie, Shuofeng Yuan, Liang Cheng, Xin Yin, Xue Zhang
Genome-wide association study (GWAS) analysis has exposed that genetic factors play important roles in COVID-19. Whereas a deeper understanding of the underlying mechanism of COVID-19 was hindered by the lack of expression of quantitative trait loci (eQTL) data specific for disease. To this end, we identified COVID-19-specific cis-eQTLs by integrating nucleotide sequence variations and RNA-Seq data
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Aichivirus A1 replicates in human intestinal epithelium and bronchial tissue: Lung–gut axis? Virus Res. (IF 5.0) Pub Date : 2024-02-23 Martin Jungbauer-Groznica, Konstantin Wiese, Irmgard Fischer, Jan Markus, Tsung-Hsien Chang, Irene Gösler, Heinrich Kowalski, Dieter Blaas, Antonio Real-Hohn
The role of aichivirus A1 (AiV-A1) in acute gastroenteritis remains controversial and in vitro data illustrating its pathogenesis in suitable human models are scarce. Here, we demonstrate that AiV-A1 isolate A846/88 replicates in ApoA1- (absorptive) and Ki-67-positive (proliferative) enterocytes in stem cell-derived human small intestinal epithelium (HIE) as well as in patient biopsy samples, but not
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Type I interferon pathway genetic variants in severe COVID-19 Virus Res. (IF 5.0) Pub Date : 2024-02-22 A.F.L. Montenegro, M.A.F. Clementino, J.N.U. Yaochite
Coronavirus Disease 2019 (COVID-19) is an infectious disease caused by SARS-CoV-2. According to the World Health Organization (WHO), there have been over 760 million reported cases and over 6 million deaths caused by this disease worldwide. The severity of COVID-19 is based on symptoms presented by the patient and is divided as asymptomatic, mild, moderate, severe, and critical. The manifestations
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Systems genetics of influenza A virus-infected mice identifies TRIM21 as a critical regulator of pulmonary innate immune response Virus Res. (IF 5.0) Pub Date : 2024-02-17 Zhuoyuan Alex Li, Akhilesh Kumar Bajpai, Ruixue Wang, Yaxin Liu, Richard J. Webby, Esther Wilk, Weikuan Gu, Klaus Schughart, Kui Li, Lu Lu
Tripartite motif 21 (TRIM21) is a cytosolic Fc receptor that targets antibody-bound, internalized pathogens for destruction. Apart from this intrinsic defense role, TRIM21 is implicated in autoimmune diseases, inflammation, and autophagy. Whether TRIM21 participates in host interactions with influenza A virus (IAV), however, is unknown. By computational modeling of body weight and lung transcriptome
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Transcriptomics of Marburg virus-infected primary proximal tubular cells reveals negative correlation of immune response and energy metabolism Virus Res. (IF 5.0) Pub Date : 2024-02-13 Benjamin Koch, Maximilian Filzmayer, Sammy Patyna, Nils Wetzstein, Sebastian Lampe, Tobias Schmid, Helmut Geiger, Patrick C. Baer, Olga Dolnik
Marburg virus, a member of the Filoviridae, is the causative agent of Marburg virus disease (MVD), a hemorrhagic fever with a case fatality rate of up to 90 %. Acute kidney injury is common in MVD and is associated with increased mortality, but its pathogenesis in MVD remains poorly understood. Interestingly, autopsies show the presence of viral proteins in different parts of the nephron, particularly
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Inhibition of EV71 replication by an interferon-stimulated gene product L3HYPDH Virus Res. (IF 5.0) Pub Date : 2024-02-13 Jian Liu, Logen Liu, Shinuan Zeng, Xiaobin Meng, Nanfeng Lei, Hai Yang, Runcai Li, Xin Mu, Xuemin Guo
Enterovirus 71 (EV71) is the common causative agent of hand-foot-mouth disease (HFMD). Despite evidence in mice model suggested that the interferon (IFN) signaling pathways play a role in defending against this virus, knowledge on the IFN-mediated antiviral response is still limited. Here we identified an IFN-stimulated gene (ISG) called L3HYPDH, whose expression inhibits EV71 replication. Mapping
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Comprehensive phytohormone metabolomic and transcriptomic analysis of tobacco (Nicotiana tabacum) infected by tomato spotted wilt virus (TSWV) Virus Res. (IF 5.0) Pub Date : 2024-02-13 Yanping Liang, Jinfeng Wan, Xin Zhang, Kunming Li, Jun Su, Min Gui, Yongzhong Li, Yating Liu
Tomato spotted wilt virus (TSWV) is ranked among the top 10 most destructive viruses globally. It results in abnormal leaf growth, stunting, and even death, significantly affecting crop yield and quality. Phytohormones play a crucial role in regulating plant-virus interactions. However, there is still limited research on the effect of TSWV on phytohormone levels, particularly growth hormones and genes
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In vitro broad-spectrum antiviral activity of MIT-001, a mitochondria-targeted reactive oxygen species scavenger, against severe acute respiratory syndrome coronavirus 2 and multiple zoonotic viruses Virus Res. (IF 5.0) Pub Date : 2024-02-05 Taehun Lim, Shivani Rajoriya, Bohyeon Kim, Augustine Natasha, Hyeonjoo Im, Hyun Soo Shim, Junsang Yoo, Jong Woo Kim, Eun-Woo Lee, Hye Jin Shin, Soon Ha Kim, Won-Keun Kim
The COVID-19 pandemic caused by SARS-CoV-2 becomes a serious threat to global health and requires the development of effective antiviral therapies. Current therapies that target viral proteins have limited efficacy with side effects. In this study, we investigated the antiviral activity of MIT-001, a small molecule reactive oxygen species (ROS) scavenger targeting mitochondria, against SARS-CoV-2 and
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Molecular insights on the coronavirus MERS-CoV interaction with the CD26 receptor Virus Res. (IF 5.0) Pub Date : 2024-02-03 Hila Failayev, Assaf Ganoth, Yossi Tsfadia
The Middle East respiratory syndrome (MERS) is a severe respiratory disease with high fatality rates, caused by the Middle East respiratory syndrome coronavirus (MERS-CoV). The virus initiates infection by binding to the CD26 receptor (also known as dipeptidyl peptidase 4 or DPP4) via its spike protein. Although the receptor-binding domain (RBD) of the viral spike protein and the complex between RBD
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PreS1BP mediates inhibition of Hepatitis B virus replication by promoting HBx protein degradation Virus Res. (IF 5.0) Pub Date : 2024-01-30 Jun Wang, Xiaoxue Yuan, Yun Wang, Yu Zhang, Ming Han, Hongping Lu, Shunai Liu, Yang Zhang, Feilin Ge, Yan Liu, Jun Cheng
Background PreS1-binding protein (PreS1BP), recognized as a nucleolar protein and tumor suppressor, influences the replication of various viruses, including vesicular stomatitis virus (VSV) and herpes simplex virus type 1 (HSV-1). Its role in hepatitis B virus (HBV) replication and the underlying mechanisms, however, remain elusive. Methods We investigated PreS1BP expression levels in an HBV-replicating
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Aged AG129 mice support the generation of highly virulent novel mouse-adapted DENV (1-4) viruses exhibiting neuropathogenesis and high lethality Virus Res. (IF 5.0) Pub Date : 2024-01-31 Gazala Siddqui, Preeti Vishwakarma, Shikha Saxena, Varun Kumar, Sneh Bajpai, Amit Kumar, Satish Kumar, Ritika Khatri, Jaskaran Kaur, Sankar Bhattacharya, Shubbir Ahmed, Gulam Hussain Syed, Yashwant Kumar, Sweety Samal
Dengue virus infection in humans ranges from asymptomatic infection to severe infection, with ∼2.5 % overall disease fatality rate. Evidence of neurological manifestations is seen in the severe form of the disease, which might be due to the direct invasion of the viruses into the CNS system but is poorly understood. In this study, we demonstrated that the aged AG129 mice are highly susceptible to dengue
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A novel antigenic epitope identified on the accessory protein NS6 of porcine deltacoronavirus Virus Res. (IF 5.0) Pub Date : 2024-01-27 Qiuge Liu, Jianxiao Wu, Na Gao, Xiaorong Zhang, Mingze Gao, Xin Zhang, Longjun Guo, Yang Wu, Da Shi, Hongyan Shi, Jianfei Chen, Li Feng
Porcine deltacoronavirus (PDCoV) is a novel enteric coronavirus that can cause vomiting, watery diarrhea in pigs and the death of piglets. The open reading frame (ORF) 5 is one of the accessory genes in PDCoV genome and encodes an accessory protein NS6. To date, the function of NS6 is still unclear. In this study, the recombinant NS6 was successfully expressed in prokaryotic expression system and purified
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Identification of a novel linear B-cell epitope on the p30 protein of African swine fever virus using monoclonal antibodies Virus Res. (IF 5.0) Pub Date : 2024-01-28 Panpan Tian, Zhuoya Sun, Mengxiang Wang, Jinxing Song, Junru Sun, Lei Zhou, Dawei Jiang, Angke Zhang, Yanan Wu, Gaiping Zhang
The outbreak of African Swine Fever (ASF) has caused huge economic losses to the pig industry. There are no safe and effective vaccines or diagnostics available. The p30 protein serves as a key target for the detection of ASFV antibodies and is an essential antigenic protein for early serological diagnosis. Here, the p30 protein was purified after being expressed in E. coli and its immunogenicity was
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Application of the zebrafish model in human viral research Virus Res. (IF 5.0) Pub Date : 2024-01-24 Jie Liu, Xiaoyi Yuan, Chunxin Fan, Guangyong Ma
Viruses are a leading cause of infectious diseases. Well-developed animal models are valuable for understanding the immune responses to viral infections and the pathogenesis of viral diseases. Zebrafish is a commonly used small vertebrate model organism with strong reproductive ability, a short life cycle, and rapid embryonic development. Moreover, zebrafish and human genomes are highly similar; they
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Increased cAMP-PKA signaling pathway activation is involved in up-regulation of CTLA-4 expression in CD4+ T cells in acute SIVmac239-infected Chinese rhesus macaques Virus Res. (IF 5.0) Pub Date : 2024-01-22 Ren-Rong Tian, Ben-Bo Liu, Ming-Liang Zhao, Yu-Jun Cai, Yong-Tang Zheng
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Rab5c promotes RSV and ADV replication by autophagy in respiratory epithelial cells Virus Res. (IF 5.0) Pub Date : 2024-01-20 Xiuli Wang, Jing Cheng, Linchao Shen, Meixi Chen, Keran Sun, Jian Li, Miao Li, Cuiqing Ma, Lin Wei
Respiratory system diseases caused by respiratory viruses are common and exert tremendous pressure on global healthcare system. In our previous studies, we found that Long non-coding RNA NRAV (Lnc NRAV) and its target molecule Rab5c plays a significant role in respiratory virus infection. However, the mechanism by which Rab5c affects virus replication remains unclear. Rab5c, a protein mainly localized
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Divergent pathogenetic outcomes in BALB/c mice following Omicron subvariant infection Virus Res. (IF 5.0) Pub Date : 2024-01-19 John M. Powers, Sarah R. Leist, Michael L. Mallory, Boyd L. Yount, Kendra L. Gully, Mark R. Zweigart, Alexis B. Bailey, Timothy P. Sheahan, Jack R. Harkema, Ralph S. Baric
Following the emergence of B.1.1.529 Omicron, the SARS-CoV-2 virus evolved into a significant number of sublineage variants that possessed numerous mutations throughout the genome, but particularly within the spike glycoprotein (S) gene. For example, the BQ.1.1 and the XBB.1 and XBB.1.5 subvariants contained 34 and 41 mutations in S, respectively. However, these variants elicited largely replication
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A computational exploration of global and temporal dynamics of selection pressure on HIV-1 Vif polymorphism Virus Res. (IF 5.0) Pub Date : 2024-01-19 Md Sakil Arman, Md Zafrul Hasan
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Characterization and genomic analysis of a broad-spectrum lytic phage PG288: A potential natural therapy candidate for Vibrio infections Virus Res. (IF 5.0) Pub Date : 2024-01-19 Chen Zhang, Xixi Li, Shen'ao Li, Han Yin, Zhe Zhao
Vibrio parahaemolyticus, an important zoonotic pathogen, can cause severe diseases and even death in aquatic animals and humans. As the widespread use of antibiotics gradually diminishes their effectiveness, phages, which can selectively lyse bacteria, are garnering increased attention as a valuable alternative antibacterial strategy. This study characterized PG288, a lytic phage utilizing V. parahaemolyticus
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The dynamic variation position and predominant quasispecies of hepatitis B virus: Novel predictors of early hepatocarcinoma Virus Res. (IF 5.0) Pub Date : 2024-01-19 Chaojun Zhang, Sanchun An, Ruibo Lv, Kezhi Li, Haizhou Liu, Jilin Li, Yanping Tang, Zhengmin Cai, Tianren Huang, Long Long, Wei Deng
To find the predictors of early HCC based on the dynamic changes of HBV quasispecies, this study utilizing the second-generation sequencing (NGS) and high-order multiplex droplet digital PCR (ddPCR) technology to examine the HBV quasispecies in serum of total 247 subjects recruited from high-incidence area of HCC. In the discovery stage, 15 non-synonymous Single Nucleotide Polymorphisms (SNPs) with
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Genomic characterization of Rocahepevirus ratti hepatitis E virus genotype C1 in Yunnan province of China Virus Res. (IF 5.0) Pub Date : 2024-01-18 Han Wu, Bingzhe Li, Bowen Yu, Linjie Hu, Lu Zhou, Jiaxiang Yin, Yihan Lu
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Mechanisms of antiviral action and toxicities of ipecac alkaloids: Emetine and dehydroemetine exhibit anti-coronaviral activities at non-cardiotoxic concentrations Virus Res. (IF 5.0) Pub Date : 2024-01-19 Viktoriya S. Sidorenko, Ira Cohen, Kunchok Dorjee, Conceição A. Minetti, David P. Remeta, Junyuan Gao, Irina Potapova, Hong Zhan Wang, Janet Hearing, Wan-Yi Yen, Hwan Keun Kim, Keiji Hashimoto, Masaaki Moriya, Kathleen G. Dickman, Xingyu Yin, Miguel Garcia-Diaz, Rajesh Chennamshetti, Radha Bonala, Francis Johnson, Amanda L. Waldeck, Thomas A. Rosenquist
The emergence of highly infectious pathogens with their potential for triggering global pandemics necessitate the development of effective treatment strategies, including broad-spectrum antiviral therapies to safeguard human health. This study investigates the antiviral activity of emetine, dehydroemetine (DHE), and congeneric compounds against SARS-CoV-2 and HCoV-OC43, and evaluates their impact on
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Oropouche virus: A neglected global arboviral threat Virus Res. (IF 5.0) Pub Date : 2024-01-16 Yuli Zhang, Xiao Liu, Zhen Wu, Shuo Feng, Ke Lu, Wenbing Zhu, Hengyi Sun, Guoyu Niu
The Oropouche virus is an important arthropod-borne virus in the Peribunyaviridae family that can cause febrile illnesses, and it is widely distributed in tropical regions such as Central and South America. Since the virus was first identified, a large number of related cases are reported every year. No deaths have been reported to date, however, the virus can cause systemic infections, including the
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Sulforaphane effectively inhibits HBV by altering Treg/Th17 immune balance and the MIF-macrophages polarizing axis in vitro and in vivo Virus Res. (IF 5.0) Pub Date : 2024-01-13 Ruqing Xu, Yue Wu, Xia Xiang, Xiaoqin Lv, Miao He, Chang Xu, Guoqi Lai, Tingxiu Xiang
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Harnessing CRISPR technology for viral therapeutics and vaccines: from preclinical studies to clinical applications Virus Res. (IF 5.0) Pub Date : 2024-01-12 Farzaneh Zahedipour, Fatemeh Zahedipour, Parvin Zamani, Mahmoud Reza Jaafari, Amirhossein Sahebkar
The CRISPR/Cas system, identified as a type of bacterial adaptive immune system, have attracted significant attention due to its remarkable ability to precisely detect and eliminate foreign genetic material and nucleic acids. Expanding upon these inherent capabilities, recent investigations have unveiled the potential of reprogrammed CRISPR/Cas 9, 12, and 13 systems for treating viral infections associated
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Mucosal-Associated Invariant T Cells are not susceptible in vitro to SARS-CoV-2 infection but accumulate into the lungs of COVID-19 patients Virus Res. (IF 5.0) Pub Date : 2024-01-13 Xiaobo Huang, Jonas Kantonen, Kirsten Nowlan, Ngoc Anh Nguyen, Suvi T. Jokiranta, Suvi Kuivanen, Nelli Heikkilä, Shamita Mahzabin, Anu Kantele, Olli Vapalahti, Liisa Myllykangas, Santtu Heinonen, Mikko I. Mäyränpää, Tomas Strandin, Eliisa Kekäläinen
Prolonged T cell lymphopenia is common in COVID-19, caused by SARS-CoV-2. While the mechanisms of lymphopenia during COVID-19 remain elusive, it is especially pronounced in a specialized innate-like T cell population called Mucosal Associated Invariant T cells (MAITs). MAITs has been suggested to express Angiotensin-Converting Enzyme 2 (ACE2), which is the well-known cellular receptor for SARS-CoV-2
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A panel of hepatitis C virus glycoproteins for the characterization of antibody responses using antibodies with diverse recognition and neutralization patterns Virus Res. (IF 5.0) Pub Date : 2024-01-11 Ana Chumbe, Marloes Grobben, Joan Capella-Pujol, Sylvie M. Koekkoek, Ian Zon, Stefan Slamanig, Sabrina J. Merat, Tim Beaumont, Kwinten Sliepen, Janke Schinkel, Marit J. van Gils
A vaccine against Hepatitis C virus (HCV) is urgently needed to limit the spread of HCV. The large antigenic diversity of the HCV glycoprotein E1E2 makes it difficult to design a vaccine but also to fully understand the antibody response after infection or vaccination. Here we designed a panel of HCV pseudoparticles (HCVpps) that cover a wide range of genetically and antigenically diverse E1E2s. We
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Molecular epidemiology to aid virtual elimination of HIV transmission in Australia Virus Res. (IF 5.0) Pub Date : 2024-01-11 Billal M. Obeng, Anthony D. Kelleher, Francesca Di Giallonardo
The Global UNAIDS 95/95/95 targets aim to increase the percentage of persons who know their HIV status, receive antiretroviral therapy, and have achieved viral suppression. Achieving these targets requires efforts to improve the public health response to increase access to care for those living with HIV, identify those yet undiagnosed with HIV early, and increase access to prevention for those most
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Temporal cytokine storm dynamics in dengue infection predicts severity Virus Res. (IF 5.0) Pub Date : 2024-01-06 Puneet Bhatt, Muralidhar Varma, Vikas Sood, Anoop Ambikan, Anup Jayaram, Naren Babu, Soham Gupta, Chiranjay Mukhopadhyay, Ujjwal Neogi
The immunopathogenesis of dengue severity is convoluted. The primary objective of the research was to examine the dynamics of cytokine storm and its correlation with disease development in individuals affected by DENV infection. Additionally, the study aimed to discover potential biomarkers that could indicate severe dengue infection and determine the most suitable timeframe for predicting the severity
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The effect of 5′ and 3′ non-translated regions on the expression of a transgene from a Newcastle disease virus vector Virus Res. (IF 5.0) Pub Date : 2024-01-06 Ishita Roy Chowdhury, Ekaterina Viktorova, Siba K. Samal, George A. Belov
Newcastle disease virus (NDV) is an avian virus and a promising vector for the development of vaccines for veterinary and human use. The optimal vaccine vector performance requires a stable high-level expression of a transgene. The foreign genes are usually incorporated in the genome of NDV as individual transcription units, whose transcription and subsequent translation of the mRNA are regulated by
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Propidium monoazide PCR, a method to determine OsHV-1 undamaged capsids and to estimate virus Lethal Dose 50 Virus Res. (IF 5.0) Pub Date : 2024-01-04 Tristan Renault, Nicole Faury, Benjamin Morga
Ostreid herpes virus 1 (OsHV-1) has been classified within the Malacoherpesviridae family from the Herpesvirales order. OsHV-1 is the etiological agent of a contagious viral disease of Pacific oysters, C. gigas, affecting also other bivalve species. Mortality rates reported associated with the viral infection vary considerably between sites and countries and depend on the age of affected stocks. A
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The avian influenza A virus receptor SA-α2,3-Gal is expressed in the porcine nasal mucosa sustaining the pig as a mixing vessel for new influenza viruses Virus Res. (IF 5.0) Pub Date : 2024-01-03 Charlotte Kristensen, Lars E. Larsen, Ramona Trebbien, Henrik E. Jensen
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Determination of the minimum infective dose of viral hemorrhagic septicemia virus (VHSV) in juvenile olive flounder, Paralichthys olivaceus using an immersion challenge model Virus Res. (IF 5.0) Pub Date : 2023-12-29 Su-Young Yoon, Yo-Seb Jang, Soo-Jin Kim, Rahul Krishnan, Myung-Joo Oh
Viral hemorrhagic septicemia virus (VHSV) affects over 80 fish species, leading to viral hemorrhagic septicemia (VHS). Horizontal VHSV transmission is widely studied, with researchers utilizing various doses to establish infection models. Infected hosts shed the virus into the environment, elevating the risk of transmission to naïve fish within the same system. This study aimed to ascertain the minimum
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Inhibition of porcine deltacoronavirus entry and replication by Cepharanthine Virus Res. (IF 5.0) Pub Date : 2023-12-30 Yumei Sun, Zhongzhu Liu, Shiyi Shen, Mengjia Zhang, Lina Liu, Ahmed H Ghonaim, Yongtao Li, Shujun Zhang, Wentao Li
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Suppression of TRIM19 by arterivirus nonstructural protein 1 promotes viral replication Virus Res. (IF 5.0) Pub Date : 2023-12-21 Chia-Ming Su, Yu Fan Hung, Junyu Tang, Mingyuan Han, Roger Everett, Dongwan Yoo
Tripartite motif (TRIM)-containing proteins are a family of regulatory proteins that can participate in the induction of antiviral cytokines and antagonize viral replication. Promyelocytic leukemia (PML) protein is known as TRIM19 and is a major scaffold protein organizing the PML nuclear bodies (NBs). PML NBs are membrane-less organelles in the nucleus and play a diverse role in maintaining cellular
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Pathological and virological findings of type I interferon receptor knockout mice upon experimental infection with Heartland virus Virus Res. (IF 5.0) Pub Date : 2023-12-16 Hikaru Fujii, Shuetsu Fukushi, Tomoki Yoshikawa, Noriyo Nagata, Satoshi Taniguchi, Masayuki Shimojima, Souichi Yamada, Hideki Tani, Akihiko Uda, Takahiro Maeki, Shizuko Harada, Takeshi Kurosu, Chang Kweng Lim, Eri Nakayama, Mutsuyo Takayama-Ito, Shumpei Watanabe, Hideki Ebihara, Shigeru Morikawa, Masayuki Saijo
Heartland virus (HRTV) causes generalized symptoms, severe shock, and multiple organ failure. We previously reported that interferon-α/β receptor knockout (IFNAR-/-) mice infected intraperitoneally with 1 × 107 tissue culture-infective dose (TCID50) of HRTV died, while those subcutaneously infected with the same dose of HRTV did not. The pathophysiology of IFNAR-/- mice infected with HRTV and the mechanism
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Applying targeted gene hybridization capture to viruses with a focus to SARS-CoV-2 Virus Res. (IF 5.0) Pub Date : 2023-12-16 Andres Ceballos-Garzon, Sophie Comtet-Marre, Pierre Peyret
Although next-generation sequencing technologies are advancing rapidly, many research topics often require selective sequencing of genomic regions of interest. In addition, sequencing low-titre viruses is challenging, especially for coronaviruses, which are the largest RNA viruses. Prior to sequencing, enrichment of viral particles can help to significantly increase target sequence information as well
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Angiotensin converting enzyme 2 does not facilitate porcine epidemic diarrhea virus entry into porcine intestinal epithelial cells and inhibits it-induced inflammatory injury by promoting STAT1 phosphorylation Virus Res. (IF 5.0) Pub Date : 2023-12-14 Zhiqiang Li, Xueqing Chen, Chang Ma, Xinyu Du, Yuanshu Zhang
ACE2 has been confirmed to be a functional receptor for SARS-CoV and SARS-CoV-2, but research on animal coronaviruses, especially PEDV, are still unknown. The present study investigated whether ACE2 plays a role in receptor recognition and subsequent infection during PEDV invasion of host cells. IPEC-J2 cells stably expressing porcine ACE2 did not increase the production of PEDV-N but inhibited its
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New anti-SARS-CoV-2 aminoadamantane compounds as antiviral candidates for the treatment of COVID-19 Virus Res. (IF 5.0) Pub Date : 2023-12-14 Daisymara Priscila de Almeida Marques, Luis Adan Flores Andrade, Erik Vinicius Sousa Reis, Felipe Alves Clarindo, Thaís de Fátima Silva Moraes, Karine Lima Lourenço, Wellington Alves De Barros, Nathália Evelyn Morais Costa, Lídia Maria de Andrade, Ágata Lopes-Ribeiro, Mariella Sousa Coêlho Maciel, Laura Cardoso Corrêa-Dias, Isabela Neves de Almeida, Thalita Souza Arantes, Vivian Costa Vasconcelos Litwinski
Here, the antiviral activity of aminoadamantane derivatives were evaluated against SARS-CoV-2. The compounds exhibited low cytotoxicity to Vero, HEK293 and CALU-3 cells up to a concentration of 1,000 µM. The inhibitory concentration (IC50) of aminoadamantane was 39.71 µM in Vero CCL-81 cells and the derivatives showed significantly lower IC50 values, especially for compounds 3F4 (0.32 µM), 3F5 (0.44 µM)
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Peroxide derivatives as SARS-CoV-2 entry inhibitors Virus Res. (IF 5.0) Pub Date : 2023-12-12 Ding-qi Zhang, Qin-hai Ma, Meng-chu Yang, Yulia Yu. Belyakova, Zi-feng Yang, Peter S. Radulov, Rui-hong Chen, Li-jun Yang, Jing-yuan Wei, Yu-tong Peng, Wu-yan Zheng, Ivan A. Yaremenko, Alexander O. Terent'ev, Paolo Coghi, Vincent Kam Wai Wong
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of the ongoing coronavirus disease 2019 (COVID-19) pandemic. Host cell invasion is mediated by the interaction of the viral spike protein (S) with human angiotensin-converting enzyme 2 (ACE2) through the receptor-binding domain (RBD). In this work, bio-layer interferometry (BLI) was used to screen a series of fifty-two peroxides
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The capsid protein of citrus leprosis virus C shows a nuclear distribution and interacts with the nucleolar fibrillarin protein Virus Res. (IF 5.0) Pub Date : 2023-12-12 Mikhail Oliveira Leastro, Vicente Pallás, Jesús Ángel Sánchez-Navarro
Brevipalpus-transmitted viruses (BTVs) have a significant negative economic impact on the citrus industry in Central and South America. Until now, only a few studies have explored the intracellular distribution and interaction of BTVs-encoded proteins with host factors, particularly for cileviruses, the main BTV responsible for the Citrus Leprosis (CL) disease. This study describes the nuclear localization
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LysZX4-NCA, a new endolysin with broad-spectrum antibacterial activity for topical treatment Virus Res. (IF 5.0) Pub Date : 2023-12-08 Ping Li, Mangmang Shen, Wenjie Ma, Xin Zhou, Jiayin Shen
The prevalence of multidrug-resistant highly virulent Klebsiella pneumoniae (MDR-hvKP) requires the development of new therapeutic agents. Herein, a novel lytic phage vB_KpnS_ZX4 against MDR-hvKP was discovered in hospital sewage. Phage vB_KpnS_ZX4 had a short latent period (5 min) and a large burst size (230 PFU/cell). It can rapidly reduce the number of bacteria in vitro and improve survival rates
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Analysis of the molecular determinants for furin cleavage of the spike protein S1/S2 site in defined strains of the prototype coronavirus murine hepatitis virus (MHV) Virus Res. (IF 5.0) Pub Date : 2023-12-09 Annette Choi, Ekaterina D. Kots, Deanndria T. Singleton, Harel Weinstein, Gary R. Whittaker
We analyzed the spike protein S1/S2 cleavage of selected strains of a prototype coronavirus, mouse hepatitis virus (MHV) by the cellular protease furin, in order to understand the structural requirements underlying the sequence selectivity of the scissile segment. The probability of cleavage of selected MHV strains was first evaluated from furin cleavage scores predicted by the ProP computer software
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Rapid increase in salivary IgA and broad recognition of spike protein following SARS-CoV-2 vaccination Virus Res. (IF 5.0) Pub Date : 2023-12-06 Kenji Ota, Hironori Sakai, Daisuke Sasaki, Fujiko Mitsumoto-Kaseida, Kei Sakamoto, Kosuke Kosai, Hiroo Hasegawa, Takahiro Takazono, Koichi Izumikawa, Hiroshi Mukae, Mya Myat Ngwe Tun, Kouichi Morita, Katsunori Yanagihara
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Gambogic acid inhibits HBx-mediated hepatitis B virus replication by targeting the DTX1-Notch signaling pathway Virus Res. (IF 5.0) Pub Date : 2023-12-05 Xu Wen, Dian Li, Peng Chen, Ming Tan, Hui Zhang, Yuting Liu, Jihua Ren, Shengtao Cheng
Background & Aims Current antiviral drugs, including nucleoside analogs and interferon, fail to eliminate the HBV covalently closed circular DNA (cccDNA), which serves as a transcript template in infected hepatocytes. Silencing the HBV X protein, which plays a crucial role in cccDNA transcription, is a promising approach to inhibit HBV replication. Therefore, the identification of novel compounds that
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A nano-luciferase expressing human coronavirus OC43 for countermeasure development Virus Res. (IF 5.0) Pub Date : 2023-12-05 Meghan V. Diefenbacher, Thomas J. Baric, David R. Martinez, Ralph S. Baric, Nicholas J. Catanzaro, Timothy P. Sheahan
The genetic diversity of the coronavirus (CoV) family poses a significant challenge for drug discovery and development. Traditional antiviral drugs often target specific viral proteins from specific viruses which limits their use, especially against novel emerging viruses. Antivirals with broad-spectrum activity overcome this limitation by targeting highly conserved regions or catalytic domains within
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Tracking SARS-CoV-2 variants during the 2023 flu season and beyond in Lebanon Virus Res. (IF 5.0) Pub Date : 2023-12-02 Ibrahim Al Kodsi, Douaa El Rayes, Jad Koweyes, Charbel Al Khoury, Kelven Rahy, Sergio Thoumi, Marc Chamoun, Hoda Haddad, Jacques Mokhbat, Sima Tokajian
Background Early SARS-CoV-2 variant detection relies on testing and genomic surveillance. The Omicron variant (B.1.1.529) has quickly become the dominant type among the previous circulating variants worldwide. Several subvariants have emerged exhibiting greater infectivity and immune evasion. In this study we aimed at studying the prevalence of the Omicron subvariants during the flu season and beyond
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Enterovirus D68 vRNA induces type III IFN production via MDA5 Virus Res. (IF 5.0) Pub Date : 2023-12-03 Chi-Chong Chio, Hio-Wai Chan, Shih-Hsiang Chen, Hsing-I Huang
Enterovirus D68 (EV-D68) primarily spreads through the respiratory tract and causes respiratory symptoms in children and acute flaccid myelitis (AFM). Type III interferons (IFNs) play a critical role in inhibiting viral growth in respiratory epithelial cells. However, the mechanism by which EV-D68 induces type III IFN production is not yet fully understood. In this study, we show that EV-D68 infection
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ASFV epitope mapping by high density peptides microarrays Virus Res. (IF 5.0) Pub Date : 2023-12-02 Cloé Desmet, Bruna Coelho-Cruz, Dora Mehn, Pascal Colpo, Ana Ruiz-Moreno
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The HC-Pro cistron of Triticum mosaic virus is dispensable for systemic infection in wheat but is required for symptom phenotype and efficient genome amplification Virus Res. (IF 5.0) Pub Date : 2023-11-30 Satyanarayana Tatineni, Jeffrey Alexander, Frank Kovacs
Triticum mosaic virus (TriMV), the type species of the genus Poacevirus in the family Potyviridae, is an economically important wheat curl mite-transmitted wheat-infecting virus in the Great Plains region of the USA. In this study, the functional genomics of helper component-proteinase (HC-Pro) encoded by TriMV was examined using a reverse genetics approach. TriMV with complete deletion of HC-Pro cistron
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Drug repurposing screens identify Tubercidin as a potent antiviral agent against porcine nidovirus infections Virus Res. (IF 5.0) Pub Date : 2023-11-30 Tianliang Wang, Guanmin Zheng, Zilu Chen, Yue Wang, Chenxu Zhao, Yaqin Li, Yixin Yuan, Hong Duan, Hongsen Zhu, Xia Yang, Wentao Li, Wenjuan Du, Yongtao Li, Dongliang Li
The emergence of new coronaviruses poses a significant threat to animal husbandry and human health. Porcine epidemic diarrhea virus (PEDV) is considered a re-emerging porcine enteric coronavirus, which causes fatal watery diarrhea in piglets. Currently, there are no effective drugs to combat PEDV. Drug repurposing screens have emerged as an attractive strategy to accelerate antiviral drug discovery