样式: 排序: IF: - GO 导出 标记为已读
-
Identification of the murine osteoblastic cell MC3T3-E1 as a permissive cell line in response to lumpy skin disease virus J. Virol. Methods (IF 3.1) Pub Date : 2024-03-12 Ting You, Meng Wang, Hongqiang Zhang, Xiangwei Wang, Xiaolong Gao, Xiangping Yin, Yuefeng Sun, Guirong Wang, Hao-tai Chen, Shanhui Ren
Lumpy skin disease virus (LSDV) is a rapidly emerging pathogen in China. Screening suitable cells for LSDV replication is vital for future research on pathogenic mechanisms and vaccine development. Previous comparative studies have identified that the rodent-derived BHK21 is a highly susceptible cell model to LSDV infection. Using western blot, indirect immune-fluorescence assay, flow cytometry, and
-
Approaching the complexity of Crimean-Congo hemorrhagic fever virus serology: A study in swine J. Virol. Methods (IF 3.1) Pub Date : 2024-03-12 Caroline Bost, Sabrina Castro-Scholten, Balal Sadeghi, David Cano-Terriza, Mario Frías, Saúl Jiménez-Ruiz, Martin H. Groschup, Ignacio García-Bocanegra, Kerstin Fischer
Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne zoonotic orthonairovirus of public health concern and widespread geographic distribution. Several animal species are known to seroconvert after infection with CCHFV without showing clinical symptoms. The commercial availability of a multi-species ELISA has led to an increase in recent serosurveillance studies as well as in the range of species
-
In-process quality control in foot-and-mouth disease vaccine production by detection of viral non-structural proteins using chemiluminescence dot blot assay J. Virol. Methods (IF 3.1) Pub Date : 2024-03-12 Uzma Jabeen, Kailash Singh Bisht, Huildore Bommanna Ranjitha, Madhusudan Hosamani, Beeragere Parameshwaraiah Sreenivasa, Pratik M. Kulkarni, Dombesara Chandrashekar Nidhi, Rajegowdanadoddi Lakshmana Amulya, Veerakyathappa Bhanuprakash, Hosur Joyappa Dechamma, Aniket Sanyal, Suresh H. Basagoudanavar
Foot-and-mouth disease (FMD) is a contagious viral disease of cloven-footed animals. Immunization with inactivated virus vaccine is effective to control the disease. Six-monthly vaccination regimen in endemic regions has proven to be effective. To enable the differentiation of infected animals from those vaccinated, non-structural proteins (NSPs) are excluded during vaccine production. While the antibodies
-
Validation of improved automated nucleic acid extraction methods for direct detection of polioviruses for global polio eradication J. Virol. Methods (IF 3.1) Pub Date : 2024-03-06 Stacey Jeffries Miles, Chelsea Harrington, Hong Sun, Ashley Deas, M. Steven Oberste, W. Allan Nix, Everardo Vega, Nancy Gerloff
Polioviruses (PV), the main causative agent of acute flaccid paralysis (AFP), are positive-sense single-stranded RNA viruses of the family . As we approach polio eradication, accurate and timely detection of poliovirus in stool from AFP cases becomes vital to success for the eradication efforts. Direct detection of PV from clinical diagnostic samples using nucleic acid (NA) extraction and real-time
-
Development and validation of the first HBV qRT-PCR assay in the Mediterranean area targeting the X region J. Virol. Methods (IF 3.1) Pub Date : 2024-03-06 Salma MADIHI, Chaimaa LAASSILI, Samia BOUKAIRA, Warda BAHA, Meriem KHYATTI, Abdelmajid ZYAD, Sanae BEN MKADDEM, Abdelouaheb BENANI
Hepatitis B virus (HBV) infection is a global public health burden and affects approximatively 300 million people around the world. Since, HBV population is represented with genetic diversity, having different viral effects. Development of a new prognosis method play a key role on the efficiency of the different treatment. The HBx protein of HBV has a potential role in Hepatocellular Carcinoma (HCC)
-
Culturing of SARS-CoV-2 from patient samples: protocol for optimal virus recovery and assessment of infectious viral load J. Virol. Methods (IF 3.1) Pub Date : 2024-03-05 Line L. Bang, Ditte R. Tornby, Stephanie T.D. Pham, Kristian Assing, Sören Möller, Yaseelan Palarasah, Lone W. Madsen, Karina G. Thomsen, Isik S. Johansen, Rune M. Pedersen, Thomas E. Andersen
Optimal sampling, preservation, and culturing of SARS-CoV-2 from COVID-19 patients are critical for successful recovery of virus isolates and to accurately estimate contagiousness of the patient. In this study, we investigated the influence of the type of sampling media, storage time, freezing conditions, sterile filtration, and combinations of these to assess the optimal pre-analytic conditions for
-
Specific molecular peak analysis by ion mobility spectrometry of volatile organic compounds in urine of COVID-19 patients: A novel diagnostic approach J. Virol. Methods (IF 3.1) Pub Date : 2024-03-05 T. Boeselt, P. Terhorst, J. Kroenig, C. Nell, M. Spielmanns, U. Boas, M. Veith, C. Vogelmeier, T. Greulich, AR Koczulla, B. Beutel, J. Huber, H. Heers
SARS-CoV-2 is usually diagnosed from naso-/oropharyngeal swabs which are uncomfortable and prone to false results. This study investigated a novel diagnostic approach to Covid-19 measuring volatile organic compounds (VOC) from patients’ urine. Between June 2020 and February 2021, 84 patients with positive RT-PCR for SARS-CoV-2 were recruited as well as 54 symptomatic individuals with negative RT-PCR
-
Diagnostic accuracy of direct reverse transcription-polymerase chain reaction using guanidine-based and guanidine-free inactivators for SARS-CoV-2 detection in saliva samples J. Virol. Methods (IF 3.1) Pub Date : 2024-03-05 Takashi Katsuno, Moto Kimura, Junko Terada-Hirashima, Yukumasa Kazuyama, Masato Ikeda, Ataru Moriya, Masami Kurokawa, Ayano Motohashi, Erina Isaka, Momoko Morishita, Kazuki Kawajiri, Kazuo Hakkaku, Susumu Saito, Yuriko Terayama, Yuriko Sugiura, Yoh Yamaguchi, Hiroshi Takumida, Hiromu Watanabe, Chie Morita, Akinari Tsukada, Yusaku Kusaba, Yoshie Tsujimoto, Akane Ishida, Keita Sakamoto, Masao Hashimoto
This study aimed to evaluate diagnostic accuracy of SARS-CoV-2 RNA detection in saliva samples treated with a guanidine-based or guanidine-free inactivator, using nasopharyngeal swab samples (NPS) as referents. Based on the NPS reverse transcription-polymerase chain reaction (RT-PCR) results, participants were classified as with or without COVID-19. Fifty sets of samples comprising NPS, self-collected
-
Optimization of duplex digital PCR for the measurement of SARS-CoV-2 RNA J. Virol. Methods (IF 3.1) Pub Date : 2024-03-04 Sang-Soo Lee, Ah Leum Kim, Jae-Hyung Park, Da-Hye Lee, Young-Kyung Bae
Quantitative PCR (qPCR) is the gold standard for detecting nucleic acid sequences specific to the target pathogen. For COVID-19 diagnosis, several molecular assays have been developed. In this study, we present an optimization strategy for the measurement of SARS-CoV-2 RNA via multiplex qPCR and digital PCR (dPCR). Compared to qPCR, both droplet and chip-based dPCR, which are known to be more sensitive
-
An optimised protocol for the expression and purification of adenovirus core protein VII J. Virol. Methods (IF 3.1) Pub Date : 2024-03-02 Ajani Athukorala, Karla J. Helbig, Brian P. McSharry, Jade K. Forwood, Subir Sarker
Adenovirus protein VII (pVII) is a highly basic core protein, bearing resemblance to mammalian histones. Despite its diverse functions, a comprehensive understanding of its structural intricacies and the mechanisms underlying its functions remain elusive, primarily due to the complexity of producing a good amount of soluble pVII. This study aimed to optimise the expression and purification of recombinant
-
Evaluation of the EasyNAT SARS-CoV-2 assay PCR test for the diagnosis of SARS-CoV-2 infection J. Virol. Methods (IF 3.1) Pub Date : 2024-02-27 Fernando Fernández-Sánchez, Elena Martín-Bautista, Francisco Rivas-Ruiz, Winnie Wu, Marilina García-Aranda, on behalf of the European RAPID-COVID group
Reverse transcription polymerase chain reaction (RT-PCR) tests are commonly utilized in commercial settings but pose challenges due to labor-intensive procedures and extended response times during peak demand. In contrast, real-time fluorescence and isothermal amplification assays using Crossing Priming Amplification (CPA) offer faster genetic material analysis, eliminate subjectivity, and require
-
Effective plant virus enrichment using carbon nanotubes and microfluidics J. Virol. Methods (IF 3.1) Pub Date : 2024-02-22 Nestor Perea Lopez, Juan Francisco Iturralde Martinez, Chad Vosburg, Edwin G. Rajotte, Cristina Rosa, Mauricio Terrones
Plant virus detection and identification in crops is a pillar for disease management, import of crop material, production of clean stock plants and basic plant virology studies. In this report, we present a platform for the enrichment and isolation of known or unknown viruses. This platform is based on carbon nanotube arrays inside a microfluidic device that can be a solution for the identification
-
-
Evaluation of the diagnostic assays detecting red sea bream iridovirus infection at different severity levels J. Virol. Methods (IF 3.1) Pub Date : 2024-02-16 Kyung-Ho Kim, Gyoungsik Kang, Won-Sik Woo, Min-Young Sohn, Ha-Jeong Son, Ju-Won Kim, Hee Jeong Kong, Young-Ok Kim, Chan-Il Park
Red sea bream iridovirus (RSIV) is a highly contagious viral infection that affects various fish species and poses a significant threat to the global aquaculture industry. Thus, accurate and timely diagnosis is paramount for sustainable management of fish health. This study rigorously evaluated the diagnostic efficacy of various polymerase chain reaction (PCR) assays, focusing on those recommended
-
Automated molecular detection of West Nile Virus in mosquito pools using the Panther Fusion system J. Virol. Methods (IF 3.1) Pub Date : 2024-02-13 Kajal M. Patel, Pushker Raj
West Nile Virus (WNV) is an arthropod-borne virus that is spread through mosquito vectors. WNV emerged in the US in 1999 and has since become endemic in the US, causing the most domestically acquired arboviral disease in the country. Mosquito surveillance for WNV is useful to monitor arboviral disease burden over time and across different locations. RT-qPCR is the preferred method for WNV surveillance
-
The development of a rapid, high-throughput neutralization assay using a SARS-CoV-2 reporter J. Virol. Methods (IF 3.1) Pub Date : 2024-02-13 Rigel Suzuki, Akifumi Kamiyama, Hayato Ito, Keita Kawashiro, Takahiro Tomiyama, Tomokazu Tamura, Saori Suzuki, Tomoharu Yoshizumi, Kiyohiko Hotta, Takasuke Fukuhara
Many methods have been developed to measure the neutralizing capacity of antibodies to SARS-CoV-2. However, these methods are low throughput and can be difficult to quickly modify in response to emerging variants. Therefore, an experimental system for rapid and easy measurement of the neutralizing capacity of antibodies against various variants is needed. In this study, we developed an experimental
-
The development of RT-RPA and CRISPR-Cas12a based assay for sensitive detection of infectious hematopoietic necrosis virus (IHNV) J. Virol. Methods (IF 3.1) Pub Date : 2024-02-07 Feixiang Rong, Hongsheng Wang, Xiaoqian Tang, Jing Xing, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Infectious hematopoietic necrosis virus (IHNV) is an economically important virus causing significant mortalities among wild and cultured salmonid fish worldwide. Rapid and sensitive diagnostic methods of IHNV are crucial for timely controlling infections. For better detection of IHNV, we have established a detection technology based on the reverse transcription and recombinase polymerase amplification
-
Development of an indirect ELISA against Orf virus using two recombinant antigens, partial B2L and F1L J. Virol. Methods (IF 3.1) Pub Date : 2024-02-07 Weihao Zheng, You Zhang, Qinglin Gu, Qian Liang, Youci Long, Qin Wu, Simei Xian
Orf is a highly contagious viral disease affecting goats and sheep. It is caused by Orf virus (ORFV) and has caused severe economic losses to the global goat industry, including in China. In this study, an indirect ELISA method for recombinant proteins based on truncated dominant antigenic epitopes of B2L and F1L genes of ORFV was established. A series of conditions and its performance were comprehensively
-
Diagnostic performance of cross-priming amplification-based lateral flow assay (CPA-LFA) and real-time PCR for koi herpesvirus (KHV) detection J. Virol. Methods (IF 3.1) Pub Date : 2024-02-02 Guk Hyun Kim, Ye Jin Jeong, Yu Gyeong Jeon, Yun Jung Yang, Joon Gyu Min, Do-Hyung Kim, Kwang Il Kim
Epizootics of Koi herpesvirus (KHV) cause mass mortality in koi carp () and common carp () worldwide. Rapid and accurate virus detection technology is crucial for preventing pathogen spread and minimizing damage. Although several diagnostic assays have been developed for KHV, the analytical and diagnostic performance of the detection methods has not been evaluated. In this study, we developed and validated
-
Visual, rapid, and cost-effective BK virus detection system for renal transplanted patients using gold nanoparticle coupled loop-mediated isothermal amplification (nanoLAMP) J. Virol. Methods (IF 3.1) Pub Date : 2024-01-28 Sunil Kumar, Srishty Raman, Kishore Sesham, Abhishek Gupta, Raj Kanwar Yadav, Asit Ranjan Mridha, Subhash Chandra Yadav
-
Genome sequencing of the mpox virus 2022 outbreak with amplicon-based Oxford Nanopore MinION sequencing J. Virol. Methods (IF 3.1) Pub Date : 2024-01-20 Annika Brinkmann, Katharina Pape, Steven Uddin, Niklas Woelk, Sophie Förster, Heiko Jessen, Janine Michel, Claudia Kohl, Lars Schaade, Andreas Nitsche
We present an amplicon-based assay for MinION Nanopore sequencing of mpox virus (MPXV) genomes from clinical specimens, obtaining high-quality results with an average genome coverage of 99% for Ct values of up to 25, and a genome coverage of 97.1% for Ct values from 25 to 30 which are challenging to sequence. This assay is easy to implement in PCR-based workflows and provides accurate genomic data
-
Specific antibody production using recombinant proteins to elucidate seed transmission and nuclear localization of Coguvirus citrulli and Coguvirus henanense in radicles of watermelon crop J. Virol. Methods (IF 3.1) Pub Date : 2024-01-19 Caterynne M. Kauffmann, Marina Vendramini, Amanda M.V. Batista, Helena B.S. Mota, Ikaro A. Andrade, Stephanny B.S. Cárdenas, Paloma S. Queiroz, Bruno A. Silva, José R. Correa, Tatsuya Nagata
Watermelon crinkle leaf-associated virus 1 (WCLaV-1) and WCLaV-2, both belonging to the genus Coguvirus (family Phenuiviridae), have been identified in watermelon plants in Brazil. To study tissue tropism and the potential for seed transmission of these viruses, we initially planned to produce specific antibodies. However, difficulties in isolating and propagating the virus in host plants hindered
-
Establishment and application of an indirect ELISA for Getah virus E2 antibody detection J. Virol. Methods (IF 3.1) Pub Date : 2024-01-14 Dong You, Yu-Ling Wang, Liang-Peng Ge, Yuan-Cheng Zhou, Jing Sun, Li-Qiao Lang, Si-Yuan Lai, Yan-Ru Ai, Ling Zhu, Zhi-Wen Xu
Getah virus (GETV) is a mosquito-transmitted disease that affects animals, causing fever, aseptic meningitis, and abortion. Its prevalence in China poses risks to both animal health and public well-being. Currently, there is a scarcity of seroepidemiological data on GETV due to the absence of commercial antibody detection kits for pigs. The aim of this study is to develop a rapid, accurate, and sensitive
-
Evaluation of long-term preservation methods for viral RNA in mosquitoes at room temperature J. Virol. Methods (IF 3.1) Pub Date : 2024-01-17 Izumi Kai, Daisuke Kobayashi, Kentaro Itokawa, Chizu Sanjoba, Kyo Itoyama, Haruhiko Isawa
Mosquitoes are important vectors of various pathogenic viruses. Almost all viruses transmitted by mosquitoes are RNA viruses. Therefore, to detect viral genes, mosquito samples must be kept at low temperatures to prevent RNA degradation. However, prolonged transport from the field to laboratory can pose challenges for temperature control. The aim of this study was to evaluate methods for preserving
-
Optimised protocols to generate high titre lentiviral vectors using a novel transfection agent enabling extended HEK293T culture following transient transfection and suspension culture J. Virol. Methods (IF 3.1) Pub Date : 2024-01-11 Saqlain Suleman, Serena Fawaz, Terry Roberts, Stuart Ellison, Brian Bigger, Michael Themis
HIV-1 based lentiviral viruses are considered powerful and versatile gene therapy vectors to deliver therapeutic genes to patients with hereditary or acquired diseases. These vectors can efficiently transduce a variety of cell types when dividing or non-dividing to provide permanent delivery and long-term gene expression. Demand for scalable manufacturing protocols able to generate enough high titre
-
-
A method for screening CDV microneutralization activity in microvolume samples J. Virol. Methods (IF 3.1) Pub Date : 2024-01-07 Xiaoyu Deng, Jiazi Su, Bo Hu, Xue Bai
Objective This study aims to establish a screening method for canine distemper virus (CDV) microneutralizing activity suitable for microvolume samples. Methods This method is based on the Indirect immunofluorescence assay (IFA) established on Vero-slam cells. First, by comparing the sensitivities of CDV neutralizing monoclonal antibody (1C42H11) and NP protein monoclonal antibody (CDV-NP) in IFA experiments
-
Quantification of low-level human cytomegalovirus and Epstein-Barr virus DNAemia by digital PCR J. Virol. Methods (IF 3.1) Pub Date : 2024-01-04 DeVon N. Hunter-Schlichting, Rachel I. Vogel, Melissa A. Geller, Heather H. Nelson
Background Digital PCR (dPCR) can quantify cell-free viral DNA (DNAemia), a biomarker of active viral infection. To accelerate epidemiologic investigation into low-level viral reactivation in chronic disease, we have evaluated the performance of dPCR to detect cytomegalovirus (CMV) and Epstein-Barr virus (EBV) DNAemia across platforms and blood matrices. Methods The droplet-based (BioRad) dPCR platform
-
Identification of a potent and specific retinoic acid-inducible gene 1 pathway activator as a Hepatitis B Virus antiviral through a novel cell-based reporter assay J. Virol. Methods (IF 3.1) Pub Date : 2024-01-02 Liping Shi, Guangyang Guo, Jinying Zhou, Zhanling Cheng, Ren Zhu, George Kukolj, Chris Li
Chronic Hepatitis B Virus (HBV) infection remains a global burden. To identify small molecule RIG-I agonists as antivirals against HBV, we developed an HBV-pgRNA-based interferon-β (IFN-β) luciferase reporter assay with high level of assay sensitivity, specificity and robustness. Through HTS screening, lead compound (JJ#1) was identified to activate RIG-I signaling pathway by inducing TBK1 phosphorylation
-
Development of a double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of VZV J. Virol. Methods (IF 3.1) Pub Date : 2023-12-27 Aiping Wang, Na Liu, Jianguo Zhao, Yan Niu, Yumei Chen, Jingming Zhou, Enping Liu, Gaiping Zhang
Background Varicella zoster virus (VZV) is the pathogen of varicella and herpes zoster, it is necessary to develop a rapid, sensitive and specific detection method for the prevention and control of related diseases. Methods We inserted the gB protein extracellular region gene (gB-ex, 1–2208 bp) of VZV into lentivirus vector, and then obtained the recombinant gB protein through mammalian expression
-
A novel VP1-based enzyme-linked immunosorbent assay revealed widespread Enterovirus G infections in Guangxi, China J. Virol. Methods (IF 3.1) Pub Date : 2023-12-23 Dalin Hong, Jinni Bian, Lingyou Zeng, Shiting Huang, Yifeng Qin, Ying Chen, Zuzhang Wei, Weijian Huang, Kang Ouyang
Enterovirus G (EV-G) has recently been shown to affect weight gain and cause neurological symptoms in piglets. However, the serological investigation of EV-G is limited. In this study, we developed a novel serological detection method based on the structural protein, VP1 of EV-G. The intra-assay and inter-assay coefficient variations were 3.2–8.9% and 2.6–8.0%, respectively. There was no cross-reaction
-
m-PIMA™ HIV1/2 VL: A suitable tool for HIV-1 and HIV-2 viral load quantification in West Africa J. Virol. Methods (IF 3.1) Pub Date : 2023-12-19 Halimatou Diop-Ndiaye, Pauline Yacine Sène, Khadidiatou Coulibaly, Marième Diallo, Sada Diallo, Karim Diop, Aissatou Sow-Ndoye, Mengue Fall, Anna Julienne Selbe Ndiaye, Evans Mathebula, Adjratou Aissatou Ba, Charlotte Lejeune, Ndeye Marie Pascaline Manga, Makhtar Camara, Cheikh Tidiane Ndour, Coumba Toure Kane
Point-of-Care for HIV viral RNA quantification seems to be a complementary strategy to the existing conventional systems. This study evaluated the performance of the m-PIMA™ HIV1/2 Viral Load for the quantification of both HIV-1 and HIV-2 RNA viral load. A total of 555 HIV-1 and 90 HIV-2 samples previously tested by Abbott RealTime HIV-1 (Abbott, Chicago, USA) and Generic HIV-2® Charge virale (Biocentric
-
Comparison of RT-LAMP and RT-qPCR assays for detecting SARS-CoV-2 in the extracted RNA and direct swab samples J. Virol. Methods (IF 3.1) Pub Date : 2023-12-15 Ramin Pourakbari, Mohammad Gholami, Ali Shakerimoghaddam, Farhad Motavalli Khiavi, Mojgan Mohammadimehr, Mehdi Shakouri Khomartash
Rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in infected patients is critical for infection control. Loop-mediated isothermal amplification (LAMP) has been demonstrated to be a rapid, simple, reliable, cost-effective and sensitive method to detect SARS-CoV-2 in a variety of samples in considerably less time than Real-Time PCR. In this study, we developed and optimized
-
Development of an accurate and rapid method for whole genome characterization of canine parvovirus J. Virol. Methods (IF 3.1) Pub Date : 2023-12-10 Emma Condon, Sofía Grecco, Ana Marandino, Jaime Aldaz, Javier Enciso, Luis Alfaro, Danilo Bucafusco, Ruben Pérez, Yanina Panzera
Canine parvovirus is a highly contagious pathogen affecting domestic dogs and other carnivores globally. Monitoring CPV through continuous genomic surveillance is crucial for mapping variability and developing effective control measures. Here, we developed a method using multiplex-PCR-next-generation sequencing to obtain full-length CPV genomes directly from clinical samples. This approach utilizes
-
Assessing the efficacy of different bead-based assays in capturing hepatitis E virus J. Virol. Methods (IF 3.1) Pub Date : 2023-12-05 Jeremy Tan, Jennifer Harlow, Jonathon Cecillon, Neda Nasheri
Hepatitis E virus (HEV) generally causes acute liver infection in humans and its transmission could be waterborne, foodborne, bloodborne, or zoonotic. To date, there is no standard method for the detection of HEV from food and environmental samples. Herein, we explored the possibility of using magnetic beads for the capture and detection of HEV. For this purpose, we employed Dynabeads M-270 Epoxy magnetic
-
Comparison of quantitative PCR and digital PCR assays for quantitative detection of infectious bronchitis virus (IBV) genome J. Virol. Methods (IF 3.1) Pub Date : 2023-12-05 Ishara M. Isham, Shahnas M. Najimudeen, Susan C. Cork, Ashish Gupta, Mohamed Faizal Abdul-Careem
The quantitative polymerase chain reaction (qPCR) technique is an extensively used molecular tool for the detection and quantification of viral genome load. However, since the qPCR assay is a relative quantification method that relies on an external calibration curve it has a lower assay precision and sensitivity. The digital PCR (dPCR) technique is a good alternative to the qPCR assay as it offers
-
Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks J. Virol. Methods (IF 3.1) Pub Date : 2023-11-28 Shizhong Zhang, Hui Dong, Fengqiang Lin, Xiaoxia Cheng, Xiaoli Zhu, Dandan Jiang, Shifeng Xiao, Shaoying Chen, Shilong Chen, Shao Wang
A multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493 bp from the viral protein 3 (VP3) gene of GPV, 300 bp
-
Detection of HIV-1 matrix protein p17 in sera of viremic and aviremic patients J. Virol. Methods (IF 3.1) Pub Date : 2023-11-28 Alberto Zani, Serena Messali, Matteo Uggeri, Carlo Bonfanti, Arnaldo Caruso, Francesca Caccuri
People living with human immunodeficiency virus type 1 (HIV-1), even if successfully treated with a combined antiretroviral therapy, display a persistent inflammation and chronic immune activation, and an increasing risk of developing cardiovascular and thrombotic events, cancers, and neurologic disorders. Accumulating evidence reveals that biologically active HIV-1 proteins may play a role in the
-
Screening and identification of B cell epitope within the major capsid protein L1 of HPV 52, using monoclonal antibodies J. Virol. Methods (IF 3.1) Pub Date : 2023-11-25 Yumei Chen, Shan Zhang, Gaiping Zhang, Jingming Zhou, Hongliang Liu, Chao Liang, Enping Liu, Xifang Zhu, Aiping Wang
The L1 protein of Human papillomavirus (HPV), the main capsid protein, induces the formation of neutralizing antibodies. In this study, HPV52 L1 protein was induced to be expressed. Monoclonal antibody (mAb) 6A7 against L1 protein were screened by cell fusion techniques. Western Blot and immunofluorescence assay (IFA) demonstrated the specificity of the mAb. The L1 protein was truncated for prokaryotic
-
Performance characteristics of Allele-Specific PCR (ASPCR) in detecting drug resistance mutations among non-B HIV-1 Variants J. Virol. Methods (IF 3.1) Pub Date : 2023-11-22 Chatté Adawaye, Joseph Fokam, Erick Ntambwe Kamangu, Derrick Tambe Ayuk Ngwese, Fabrice Susin, Ali Mahamat Moussa, BertinTchombou Hig-Zounet, Joseph Mad-Toingué, Abdelsalam Tidjani, Dolores Vaira, Michel Moutschen
Allele-Specific Polymerase Chain Reaction (ASPCR) is an affordable point-mutation assay whose validation could improve the detection of HIV-1 drug resistance mutations (DRMs) in resource-limited settings (RLS). We assessed the performance of ASPCR onforty-four non-B HIV-1 plasma samples from patients who were ARV treated in failure in N’Djamena-Chad. Viral RNA was reverse-transcribed and amplified
-
A sensitive luciferase reporter assay for the detection of infectious African swine fever virus J. Virol. Methods (IF 3.1) Pub Date : 2023-11-19 Kemal Mehinagic, Matthias Liniger, Maksym Samoilenko, Nick Soltermann, Markus Gerber, Nicolas Ruggli
African swine fever virus (ASFV) is a complex DNA virus causing severe hemorrhagic disease in domestic pigs and wild boar. The disease has spread worldwide, with important socio-economic consequences. Early virus detection and control measures are crucial as there are no effective vaccines nor antivirals on the market. While the diagnosis of ASFV is fast and based primarily on qPCR, the detection of
-
Comparison of Polymerase Chain Reaction (PCR) assay performance in detecting Decapod penstylhamaparvovirus 1 in penaeid shrimp J. Virol. Methods (IF 3.1) Pub Date : 2023-11-19 Arun K. Dhar, Roberto Cruz-Flores, Hung N. Mai, Janet Warg
Decapod Penstylhamaparvovirus 1, commonly known as infectious hypodermal and hematopoietic necrosis virus (IHHNV), remains an economically important viral pathogen for penaeid shrimp aquaculture due to its effects on growth performance. The World Organization for Animal Health (WOAH, Paris, France) recommended methods for the detection of IHHNV include both conventional and real-time PCR. However,
-
Novel high-coverage primers for detection of canine morbillivirus by end-point and real-time RT-PCR assays J. Virol. Methods (IF 3.1) Pub Date : 2023-11-17 Alice Silveira Becker, Thaísa Regina Rocha Lopes, Natália Hettwer Pedroso, José Valter Joaquim Silva Júnior, Rudi Weiblen, Eduardo Furtado Flores
Canine distemper virus (CDV) is a major threat to domestic dogs and wildlife worldwide. Molecular assays are the most sensitive and specific tests to diagnose the disease, however, the high CDV genetic variability may compromise laboratory diagnosis. Herein, we designed a high-coverage primer set for end-point (RT-PCR) and real-time (RT-qPCR) for CDV detection. Initially, we collected 194 complete/near-complete
-
Purification of hepatitis C virus core protein in non-denaturing condition J. Virol. Methods (IF 3.1) Pub Date : 2023-11-16 Kyo Izumida, Yumiko Hara, Yukio Furukawa, Kotaro Ishida, Keisuke Tabata, Eiji Morita
Hepatitis C virus (HCV) is the major cause of chronic hepatitis and hepatocellular carcinoma. Among its structural proteins, the HCV core protein has been implicated in liver disease. Understanding the role of HCV core proteins in viral diseases is crucial to elucidating disease mechanisms and identifying potential drug targets. However, purification challenges hinder the comprehensive elucidation
-
The value of point-of-care tests for the detection of SARS-CoV-2 RNA or antigen in bronchoalveolar lavage fluid J. Virol. Methods (IF 3.1) Pub Date : 2023-11-07 Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Background Transmission of SARS-CoV-2 from donor to recipient is a clinically relevant risk for developing severe COVID-19 after lung transplantation (LTx). This risk of iatrogenic transmission can be reduced by timely detection of viral RNA or antigen in samples of bronchoalveolar lavage (BAL) fluid obtained at the time of lung procurement. We aimed to retrospectively evaluate the detection of SARS-CoV-2
-
Development and evaluation of a monoclonal antibody-based blocking ELISA to detect antibodies against the E2 protein of bovine viral diarrhea virus-1 J. Virol. Methods (IF 3.1) Pub Date : 2023-11-11 Xinhuan Liu, Zilong Cheng, Wenwen Zhang, Li Mao, Zihao Pan, Leilei Yang, Maojun Liu, Yunfeng Long, Juan Bai, Wenliang Li
With the rapid development of cattle industry, bovine viral diarrhea virus (BVDV) is becoming widespread in China, which causes serious economic losses to the industry. Effective vaccination and viral surveillance are critical for the prevent and control of BVDV infection. In the present study, the immunogenic domain of E2 protein of BVDV-1 was expressed by prokaryotic pET-28a vector. Monoclonal antibodies
-
Development and application of monoclonal antibody-based dot-ELISA and colloidal gold immunochromatographic strip for rapid, specific, and sensitive detection of tomato brown rugose fruit virus J. Virol. Methods (IF 3.1) Pub Date : 2023-11-07 Xinru Zhao, Jiayu Wu, Ziyue Ma, Yujie Shi, Zhu Fang, Jianxiang Wu, Xiuling Yang, Xueping Zhou
Tomato brown rugose fruit virus (ToBRFV) is an emerging tobamovirus that has become a great concern to tomato production industry. Due to the lack of resistant cultivars, precise detection of ToBRFV is essential to prevent the spread of ToBRFV. In this study, we produced highly sensitive and specific monoclonal antibodies against ToBRFV and established dot-enzyme-linked immunosorbent assay (dot-ELISA)
-
Visualizing intracellular sialidase activity of influenza A virus neuraminidase using a fluorescence imaging probe J. Virol. Methods (IF 3.1) Pub Date : 2023-10-31 Koki Amano, Yuuki Kurebayashi, Tadanobu Takahashi, Yutaka Narimichi, Tadamune Otsubo, Kiyoshi Ikeda, Akira Minami, Hideyuki Takeuchi
In influenza A virus-infected cells, newly synthesized viral neuraminidases (NAs) transiently localize at the host cell Golgi due to glycosylation, before their expression on the cell surface. It remains unproven whether Golgi-localized intracellular NAs exhibit sialidase activity. We have developed a sialidase imaging probe, [2-(benzothiazol-2-yl)−5-(non-1-yn-1-yl) phenyl]-α-D-N-acetylneuraminic acid
-
Approaches to produce and characterize recombinant protein VP1-2A of HAV for serological rapid test application J. Virol. Methods (IF 3.1) Pub Date : 2023-11-02 Michel V F Sucupira, Ana P C Argondizzo, Mariana Miguez, Anna E V de Araujo, Leila B R Silva, Marcelle B Mello, Christiane F S Marques, Danielle R A Brito e Cunha, Renata C Bastos, Vanessa S de Paula, Luciane A Amado Leon
Studies reporting the expression of hepatitis A virus (HAV) structural proteins, specifically recombinant VP1-2A containing an immunogenic activity, use the Escherichia coli system. Recombinant HAV proteins may represent a source of less expensive antigens for application in different diagnostic platforms. However, the formation of insoluble aggregates is an obstacle to obtaining large amounts of HAV
-
Metagenomics in the fight against zoonotic viral infections: A focus on SARS-CoV-2 analogues J. Virol. Methods (IF 3.1) Pub Date : 2023-10-31 Atif Khurshid Wani, Chirag Chopra, Daljeet Singh Dhanjal, Nahid Akhtar, Himanshu Singh, Poorvi Bhau, Anjuvan Singh, Varun Sharma, Rafael Silvio Bonilha Pinheiro, Juliana Heloisa Pinê Américo-Pinheiro, Reena Singh
-
Development and validation of a highly specific in-house chemiluminescent-based serological assay for the detection of antibodies directed against the human monkeypox virus J. Virol. Methods (IF 3.1) Pub Date : 2023-10-27 Christian Therrien, Jérémie Prévost, Antoine Cloutier Blais, Sonia Turcotte, Gabrielle Gendron-Lepage, Andrés Finzi, Judith Fafard
Abstract not available
-
Recovery of complete genome sequences of Crimean-Congo haemorrhagic fever virus (CCHFV) directly from clinical samples: A comparative study between targeted enrichment and metagenomic approaches J. Virol. Methods (IF 3.1) Pub Date : 2023-10-23 Jake D’Addiego, Nadina Wand, Babak Afrough, Tom Fletcher, Yohei Kurosaki, Hakan Leblebicioğlu, Roger Hewson
Crimean-Congo haemorrhagic fever (CCHF) is the most prevalent human tick-borne viral disease, endemic to the Balkans, Africa, Middle East and Asia. There are currently no licensed vaccines or effective antivirals against CCHF. CCHF virus (CCHFV) has a negative sense segmented tripartite RNA genome consisting of the small (S), medium (M) and large (L) segments. Depending on the segment utilised for
-
Label-free imaging of nuclear membrane for analysis of nuclear import of viral complexes J. Virol. Methods (IF 3.1) Pub Date : 2023-10-22 Andrew Ten Eyck, Yen-Cheng Chen, Levi Gifford, Dariana Torres-Rivera, Eva L. Dyer, Gregory B. Melikyan
HIV-1 enters the nucleus of non-dividing cells through the nuclear pore complex where it integrates into the host genome. The mechanism of HIV-1 nuclear import remains poorly understood. A powerful means to investigate the docking of HIV-1 at the nuclear pore and nuclear import of viral complexes is through single virus tracking in live cells. This approach necessitates fluorescence labeling of HIV-1
-
Mammalian cells-based platforms for the generation of SARS-CoV-2 virus-like particles J. Virol. Methods (IF 3.1) Pub Date : 2023-10-21 Ghada Elfayres, Ricky Raj Paswan, Laura Sika, Marie-Pierre Girard, Soumia Khalfi, Claire Letanneur, Kéziah Milette, Amita Singh, Gary Kobinger, Lionel Berthoux
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of COVID-19. Though many COVID-19 vaccines have been developed, most of them are delivered via intramuscular injection and thus confer relatively weak mucosal immunity against the natural infection. Virus-Like Particles (VLPs) are self-assembled nanostructures composed of key viral structural proteins, that mimic the
-
Fast preparation of high-quality viral dsRNA from fungal tissue by commercial nucleic acid extraction kits J. Virol. Methods (IF 3.1) Pub Date : 2023-10-17 Tobias Lutz, Mareike Jaeckel, Birgit Hadeler, Cornelia Heinze
The genomes of most known mycoviruses consist of double stranded RNA (dsRNA) or single stranded RNA (ssRNA). Therefore, for all aspects of mycovirology, the research is highly dependent on the quality and quantity of RNA either by the extraction of genomic dsRNA or dsRNA as a replicating intermediate. A common procedure to extract dsRNA is its binding on a cellulose matrix after a phenol/chloroform
-
Vaccination against tick-borne encephalitis elicits a detectable NS1 IgG antibody response J. Virol. Methods (IF 3.1) Pub Date : 2023-10-13 Rahel Ackermann-Gäumann, Arthur Brêchet, Jan Smetana, Jiři Salát, Reto Lienhard, Antony Croxatto, Petra Polcarová, Roman Chlíbek, Daniel Růžek
Vaccine-induced protection against tick-borne encephalitis virus (TBEV) is mediated by antibodies to the viral particle/envelope protein. The detection of non-structural protein 1 (NS1) specific antibodies has been suggested as a marker indicative of natural infections. However, recent work has shown that TBEV vaccines contain traces of NS1, and immunization of mice induced low amounts of NS1-specific
-
Utility of glutaraldehyde-fixed turkey red blood cells for influenza virus detection after 18 months of storage J. Virol. Methods (IF 3.1) Pub Date : 2023-10-01 Shailesh D. Pawar, Deeksha S. Tare, Sadhana S. Kode, Sachin S. Keng
Turkey red blood cells (tRBCs) are an essential reagent used in the laboratory diagnosis of influenza viruses. Fresh tRBCs when stored at 4 °C have a shelf life of less than a week. Previous studies have shown the utility of glutaraldehyde-fixed tRBCs, with an increased shelf life, for use in hemagglutination (HA) assays. In the present study, we report their functionality after storage for 18 months
-
A reverse transcription-multiplex PCR strategy devised for concomitant detection and differentiation of foot and mouth disease virus serotypes O, A and Asia 1 in India J. Virol. Methods (IF 3.1) Pub Date : 2023-10-01 Jajati Keshari Mohapatra, Manoranjan Rout, Saravanan Subramaniam, Priyabrata Giri, Shyam Singh Dahiya, Sagar Sangam Rautaray, Jitendra Kumar Biswal, Nihar Ranjan Sahoo, Rabindra Prasad Singh
Serotype identification occupies the central part of foot and mouth disease (FMD) diagnosis workflow and vaccination decision tree. In this study, a reverse transcription-multiplex PCR (RT-mPCR) strategy wherein three assays with unique combinations of serotype specific primers targeting the VP1 region was developed to differentiate FMD virus serotypes O, A and Asia 1 based on differential size of
-
Development of a high-throughput flow cytometric neutralization assay to screen for human enterovirus A71 (EVA71) neutralizing antibodies J. Virol. Methods (IF 3.1) Pub Date : 2023-09-30 Artit Wongsa, Thongkoon Priengprom, Jantip Saelee, Chintana Phawong, Boonrat Tassaneetrithep
The conventional method for screening neutralizing antibodies to human enterovirus A71 (EVA71) (microneutralization assay) is time consuming and requires an expert to perform manual evaluation. An automated neutralization assay could shorten the testing time, improve reproducibility, and provide automatic analysis. This study aimed to develop a high-throughput flow cytometric neutralization assay to
-
Improved virus concentration methods for wash waters from decontamination of permeable and non-permeable surfaces J. Virol. Methods (IF 3.1) Pub Date : 2023-09-29 Brittany N. Hurst, Asja Korajkic, Adin Pemberton, Brian R. McMinn
Surface decontamination is a method of using wash water to decontaminated surfaces preventing transmission of biological contaminants that can pose potential health risks to responders and the public. However, the risks associated with handling used wash water are largely unknown due to the lack of effective methodology to screen for pathogenic microorganisms present in these samples, especially viral