-
Bovine parainfluenza virus type 3 infections induce ER stress-mediated autophagy to facilitate virus replication Vet. Microbiol. (IF 3.3) Pub Date : 2024-03-12 Yu Han, Chongyang Wang, Chongsheng Bai, Enying Diao, Binxuan Yuan, Kejia Lu, Xiaoyu Dong, Riteng Zhang, Bin Han, Haijin Liu, Juan Wang, Xinglong Wang, Sa Xiao, Zengqi Yang
Bovine Parainfluenza Virus Type 3 (BPIV3) serves as a crucial pathogen in cattle, adept at triggering severe respiratory symptoms. This investigation explores the intricate interplay of endoplasmic reticulum stress (ER stress), unfolded protein response (UPR), and autophagy upon BPIV3 infection. In this study, we initially confirm a substantial increase in glucose regulatory protein 78 (GRP78) expression
-
Novel variant infectious bursal disease virus diminishes FAdV-4 vaccination and enhances pathogenicity of FAdV-4 Vet. Microbiol. (IF 3.3) Pub Date : 2024-03-11 Zhishan Liang, Mei Leng, Jiamin Lian, Yazheng Chen, Qi Wu, Feng Chen, Zhanxin Wang, Wencheng Lin
Infectious bursal disease virus (IBDV) caused an acute and highly contagious infectious disease characterized by severe immunosuppression, causing considerable economic losses to the poultry industry globally. Although this disease was well-controlled under the widely use of commercial vaccines in the past decades, the novel variant IBDV strains emerged recently because of the highly immunized-selection
-
Analysis of H5N8 influenza virus infection in chicken with mApple reporter genes in vivo and in vitro Vet. Microbiol. (IF 3.3) Pub Date : 2024-03-11 Wei Song, Li Zhao, Sairu Liu, Yusheng Jia, Lulu Ma, Ming Liao, Manman Dai
H5N8 highly pathogenic avian influenza virus (HPAIV) has caused huge losses to the global poultry industry and critically threatens public health. Chickens are the important host for the transmission. However, the distribution of H5N8 avian influenza virus (AIV) in chicken and the infected cell types are limitedly studied. Therefore, in this study, we detected viral replication and infection by generating
-
IFITM1 and IFITM2 inhibit the replication of senecavirus A by positive feedback with RIG-I signaling pathway Vet. Microbiol. (IF 3.3) Pub Date : 2024-03-11 Huizi Li, Ming Chen, Tingting Zheng, Xiaoling Lei, Cunhao Lin, Shuo Li, Jiacong Mo, Zhangyong Ning
The role of host factors in the replication of emerging senecavirus A (SVA) which induced porcine idiopathic vesicular disease (PIVD) distributed worldwide remains obscure. Here, interferon-induced transmembrane (IFITM) protein 1 and 2 inhibit SVA replication by positive feedback with RIG-I signaling pathway was reported. The expression levels of IFITM1 and IFITM2 increased significantly in SVA infected
-
Identification of ISVlu1-derived translocatable units containing optrA and/or fexA genes generated by homologous or illegitimate recombination in Lactococcus garvieae of porcine origin Vet. Microbiol. (IF 3.3) Pub Date : 2024-03-09 Qin Yang, Xiumei Wang, Stefan Schwarz, Yao Zhu, Wanjiang Zhang
The gene encodes an ABC-F protein which confers cross-resistance to oxazolidinones and phenicols. Insertion sequence IS, a novel IS-family member, was recently reported to be involved in the transmission of in . However, the role of IS in mobilizing resistance genes has not yet fully explored. In this study, two complete and three truncated copies of IS were found on plasmid pBN62-optrA from . Analysis
-
Comparative analysis of antimicrobial resistance phenotype and genotype of Riemerella anatipestifer Vet. Microbiol. (IF 3.3) Pub Date : 2024-03-08 Hongyan Dong, Shanyuan Zhu, Fan Sun, Qi Feng, Changming Guo, Zhi Wu, Shuang Wu, Anping Wang, Shengqing Yu
is one of the important bacterial pathogens that threaten the waterfowl farming industry. In this study, 157 suspected strains were isolated from diseased ducks and geese from seven regions of China during 2019–2020, and identified using multiple polymerase chain reaction (PCR). Antimicrobial susceptibility tests and whole-genome sequence (WGS) analysis were then performed for comparative analysis
-
Discovery of the tigecycline resistance gene cluster tmexCD3-toprJ1 in Pasteurella multocida strains isolated from pigs in China Vet. Microbiol. (IF 3.3) Pub Date : 2024-03-08 Congcong Shi, Zhanwei Zhu, Yuyao Shang, Wenbo Song, Jie Yang, Haixin Bi, Zihao Wang, Rui Xie, Mengfei Zhao, Lin Hua, Huanchun Chen, Bin Wu, Zhong Peng
is a leading cause of respiratory disorders in pigs. However, the genotypes and antimicrobial resistance characteristics of from pigs in China have not been reported frequently. In this study, we investigated 381 porcine strains of collected in China between 2013 and 2022. These strains were assigned to capsular genotypes A (69.55%, n = 265), D (27.82%, n =106), and F (2.62%, n = 10); or lipopolysaccharide
-
Characterization of in vitro viral neutralization targets of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) in alveolar macrophage and evaluation of protection potential against HP-PRRSV challenged based on combination of HP-PRRSV-structure proteins in vitro Vet. Microbiol. (IF 3.3) Pub Date : 2024-03-05 Bing Liu, Xu Zheng, Xiangyu Sun, Bingjie Wan, Jianhui Dong, Zhaobin Zhou, Yuchen Nan, Chunyan Wu
Porcine reproductive and respiratory syndrome virus (PRRSV) poses a significant threat to the global pork industry, resulting in substantial economic losses. Current control measures rely on modified live virus (MLV) vaccines with safety concerns. However, the lack of consensus on protective PRRSV antigens is impeding the development of effective and safety subunit vaccines. In this study, we conducted
-
Use of a local anaesthetic and antiseptic wound formulation for the treatment of lambs naturally infected with Orf virus Vet. Microbiol. (IF 3.3) Pub Date : 2024-03-03 Álex Gómez, Delia Lacasta, María Teresa Tejedor, Marta Ruiz de Arcaute, Juan José Ramos, Héctor Ruiz, Aurora Ortín, Sergio Villanueva-Saz, Ramsés Reina, Pablo Quílez, Teresa Navarro, Maite Verde, Marta Borobia, Peter Andrew Windsor
Contagious ecthyma (CE) is a worldwide highly contagious zoonotic viral skin disease of sheep and goats. Treatment for Orf virus (ORFV) infection usually involves topical and oral antibiotics. An anaesthetic and antiseptic topical gel (Multisolfen® or Tri-Solfen®; MS®, Medical Ethics, Australia) has been documented as an efficacious therapy for lesions from mucosal and epithelial viral infections in
-
Rotavirus infection inhibits SLA-I expression on the cell surface by degrading β2 M via ERAD-proteasome pathway Vet. Microbiol. (IF 3.3) Pub Date : 2024-03-02 Zhaoyang Jing, Ling Wu, Yudi Pan, Liaoyuan Zhang, Xin Zhang, Da Shi, Hongyan Shi, Jianfei Chen, Zhaoyang Ji, Jiyu Zhang, Tingshuai Feng, Jin Tian, Li Feng
Group A Rotavirus (RVA) is a major cause of diarrhea in infants and piglets. β2-microglobulin (β2 M), encoded by the gene, serves as a crucial subunit of the major histocompatibility complex class I (MHC-I) molecules. β2 M is indispensable for the transport of MHC-I to the cell membrane. MHC-I, also known as swine leukocyte antigen class I (SLA-I) in pigs, presents viral antigens to the cell surface
-
H5N1 high pathogenicity avian influenza virus in migratory birds exhibiting low pathogenicity in mallards increases its risk of transmission and spread in poultry Vet. Microbiol. (IF 3.3) Pub Date : 2024-03-02 Qiuyan Mao, Zhixin Li, Yuecheng Li, Yaxin Zhang, Shuo Liu, Xin Yin, Cheng Peng, Rui Ma, Jinping Li, Guangyu Hou, Wenming Jiang, Hualei Liu
In 2020, an H5N1 avian influenza virus of clade 2.3.4.4b was detected in Europe for the first time and was spread throughout the world by wild migratory birds, resulting in the culling of an unprecedented number of wild birds and poultry due to the epidemic. In February 2023, we isolated and identified a strain of H5N1 high pathogenicity avian influenza virus from a swab sample from a grey crane in
-
PEDV nucleocapsid antagonizes zinc-finger antiviral protein by disrupting the interaction with its obligate co-factor, TRIM25 Vet. Microbiol. (IF 3.3) Pub Date : 2024-02-29 Jantakarn Chuenchat, Supasek Kardkarnklai, Jaraspim Narkpuk, Benjamas Liwnaree, Anan Jongkaewwattana, Peera Jaru-Ampornpan, Suttipun Sungsuwan
The genomes of many pathogens contain high-CpG content, which is less common in most vertebrate host genomes. Such a distinct di-nucleotide composition in a non-self invader constitutes a special feature recognized by its host’s immune system. The zinc-finger antiviral protein (ZAP) is part of the pattern recognition receptors (PRRs) that recognize CpG-rich viral RNA and subsequently initiate RNA degradation
-
PI3K/AKT mediated De novo fatty acid synthesis regulates RIG-1/MDA-5-dependent type I IFN responses in BVDV-infected CD8+T cells Vet. Microbiol. (IF 3.3) Pub Date : 2024-02-28 Shan-shan Liu, Tong-tong Bai, Tao-lin Que, An Luo, Yu-xin Liang, Yu-xin Song, Tian-yi Liu, Jin-wei Chen, Jing Li, Nan Li, Ze-chen Zhang, Nan-nan Chen, Yu Liu, Ze-cai Zhang, Yu-long Zhou, Xue Wang, Zhan-bo Zhu
Bovine viral diarrhea virus (BVDV) has caused massive economic losses in the cattle business worldwide. Fatty acid synthase (FASN), a key enzyme of the fatty acid synthesis (FAS) pathway, has been shown to support virus replication. To investigate the role of fatty acids (FAs) in BVDV infection, we infected CD8T lymphocytes obtained from healthy cattle with BVDV in vitro. During early cytopathic (CP)
-
Bacillus subtilis-597 induces changes in lung pathology and inflammation during influenza A virus infection in pigs Vet. Microbiol. (IF 3.3) Pub Date : 2024-02-24 Katrine Winther, Charlotte Kristensen, Betina Lyngfeldt Henriksen, Lea Hübertz Birch Hansen, Pia Ryt-Hansen, Gisle Vestergaard, Kerstin Skovgaard, Dorthe Sandvang, Erik Juncker Boll, Andrew R. Williams, Lars E. Larsen
In recent years, it has become apparent that imbalances in the gastrointestinal system can impact organs beyond the intestine such as the lungs. Given the established ability of probiotics to modulate the immune system by interacting with gastrointestinal cells, our research aimed to investigate whether administering the probiotic strain -597 could mitigate the outcome of influenza virus infection
-
Tegument protein UL3 of bovine herpesvirus 1 suppresses antiviral IFN-I signaling by targeting STING for autophagic degradation Vet. Microbiol. (IF 3.3) Pub Date : 2024-02-22 Fachao Sun, Wenqing Ma, Hongmei Wang, Hongbin He
Bovine herpesvirus 1 (BoHV-1) is a highly contagious pathogen which causes infectious bovine rhinotracheitis in cattle worldwide. Although it has the ability to evade the host's antiviral innate immune response and establish persistent latent infections, the mechanisms are not fully understood, especially the function of the tegument protein to escape innate immunity and participate in viral replication
-
Characterization of Actinobacillus pleuropneumoniae biovar 2 isolates reportedly reacted with the serovar 4 antiserum, and development of a multiplex PCR for O-antigen typing Vet. Microbiol. (IF 3.3) Pub Date : 2024-02-22 Ho To, Jaime Maldonado, Nobuyuki Tsutsumi, Marcelo Gottschalk, Joachim Frey, Shinya Nagai
We have analyzed the capsule (CPS) and the lipopolysaccharide O-Antigen (O-Ag) biosynthesis loci of twelve Spanish field isolates of biovar 2, eleven of them previously typed serologically as serovar 4 and one non-typable (NT) (Maldonado et al., 2009, 2011). These isolates have the common core genes of the type I CPS locus, sharing >98% identity with those of serovar 2. However, the former possesses
-
-
Detection of a novel genotype of Chlamydia buteonis in falcons from the Emirates Vet. Microbiol. (IF 3.3) Pub Date : 2024-02-16 F. Vorimore, R. Aaziz, L. Al Qaysi, U. Wernery, N. Borel, K. Sachse, K. Laroucau
are a family of obligate intracellular bacterial pathogens that affect both humans and animals. Recently, a new species named was isolated from hawks. In this study, we aimed to investigate the prevalence of in 60 falcons that underwent a routine health check at a specialized clinic in Dubai, United Arab Emirates. Using real-time PCR, we analyzed cloacal and tracheal swabs from these birds and found
-
Fatal leptospirosis in endangered Barbary macaques (Macaca sylvanus) kept in captivity: Assessing the role of sympatric rodents Vet. Microbiol. (IF 3.3) Pub Date : 2024-02-14 Adrián Beato-Benítez, David Cano-Terriza, Moisés Gonzálvez, Remigio Martínez, Iratxe Pérez-Cobo, María José Ruano, Rafael Guerra, Elena Mozos-Mora, Ignacio García-Bocanegra
Between December 2020 and January 2021, an outbreak of acute mortality in endangered Barbary macaques () kept in captivity was detected in a zoo in Spain. The main findings observed in the two fatally affected animals at evaluation were jaundice, renal tubular necrosis and interstitial nephritis. spp. infection was confirmed by real time PCR (qPCR) in different tissues in both individuals. Analyses
-
Transcriptomic analysis of the effects of tylosin on the protective immunity provided by the Mycoplasma gallisepticum vaccine Vaxsafe MG ts-304 Vet. Microbiol. (IF 3.3) Pub Date : 2024-02-13 Kanishka I. Kamathewatta, Sathya N. Kulappu Arachchige, Neil D. Young, Anna Kanci Condello, Nadeeka K. Wawegama, Glenn F. Browning
The antimicrobial tylosin is commonly used to control mycoplasma infections, sometimes in combination with vaccination. However, the efficacy of a live mycoplasma vaccine, when combined with subsequent antimicrobial treatment, against the effects of subsequent infection with a virulent strain is unknown. This study employed differential gene expression analysis to evaluate the effects of tylosin on
-
Nucleocytoplasmic shuttling of BEFV M protein-modulated by lamin A/C and chromosome maintenance region 1 through a transcription-, carrier- and energy-dependent pathway Vet. Microbiol. (IF 3.3) Pub Date : 2024-02-10 Yu-Kang Chang, Yi-Jyum Lin, Ching-Yuan Cheng, Pei-Chien Tsai, Chi-Young Wang, Brent L. Nielsen, Hung-Jen Liu
This study demonstrates for the first time that the matrix (M) protein of BEFV is a nuclear targeting protein that shuttles between the nucleus and the cytoplasm in a transcription-, carrier-, and energy-dependent manner. Experiments performed in both intact cells and digitonin-permeabilized cells revealed that M protein targets the nucleolus and requires carrier, cytosolic factors or energy input
-
Genetic comparison of the Mycoplasma gallisepticum 6/85 vaccine strain and 6/85-like field isolates Vet. Microbiol. (IF 3.3) Pub Date : 2024-02-09 Spencer A. Leigh, Jeff D. Evans
infection in poultry leads to disease and pathology that can reduce producer profits. Live attenuated vaccines are available that can limit or completely prevent the effects of infection. Field isolates that are genetically related to the attenuated vaccine strains have been isolated, raising the question of whether the attenuation of the vaccine strains is limited and can lead the strains to revert
-
Potassium diformate alleviated inflammation of IPEC-J2 cells infected with EHEC Vet. Microbiol. (IF 3.3) Pub Date : 2024-02-09 Ying Lin, Jun-Lei Zhai, Ya-Ting Wang, Ping-Ting Guo, Jing Zhang, Chang-Kang Wang, Ling Jin, Yu-Yun Gao
Potassium diformate (KDF) is a kind of formate, which possesses the advantages of antimicrobial activity, growth promotion and preventing diarrhea in weaned piglets. However, the researches of KDF in animal production mostly focused on apparent indexes such as growth performance and the mechanisms of KDF on intestinal health have not been reported. Thus, porcine small intestinal epithelial cells (IPEC-J2)
-
Antimicrobial susceptibility of mastitis pathogens isolated from North American dairy cattle, 2011-2022 Vet. Microbiol. (IF 3.3) Pub Date : 2024-02-08 Michael T. Sweeney, Lacie Gunnett, Dipu Mohan Kumar, Bryce L. Lunt, Véronique Moulin, Michele Barrett, Abhijit Gurjar, Elizabeth Doré, Juan R. Pedraza, Don Bade, Chandra Machin
A total of 10,890 bacterial isolates of and isolated as etiological agents from dairy cows with mastitis by 29 veterinary laboratories across North America between 2011 and 2022 were tested for antimicrobial susceptibility by broth microdilution to ampicillin, cefoperazone, ceftiofur, cephalothin, erythromycin, oxacillin, penicillin-novobiocin and pirlimycin according to CLSI standards. Using available
-
UBE2J1 promotes ALV-A proviral DNA synthesis through the STAT3/IRF1 signaling pathway Vet. Microbiol. (IF 3.3) Pub Date : 2024-02-07 Xingming Wang, Shiling Zheng, Chun Fang, Xiongyan Liang, Yuying Yang
The ubiquitin-binding enzyme E2J1 is located on the endoplasmic reticulum membrane. It plays a role in transport throughout the process of ubiquitination. In mammals, UBE2J1 can promote RNA virus replication. However, the biological function of chicken UBE2J1 is unclear. In this study, chicken UBE2J1 was cloned for the first time, and UBE2J1 overexpression and shRNA knockdown plasmids were constructed
-
Adaptation of African swine fever virus to MA-104 cells: Implications of unique genetic variations Vet. Microbiol. (IF 3.3) Pub Date : 2024-02-07 Theeradej Thaweerattanasinp, Challika Kaewborisuth, Ratchanont Viriyakitkosol, Janya Saenboonrueng, Asawin Wanitchang, Nathiphat Tanwattana, Chutima Sonthirod, Duangjai Sangsrakru, Wirulda Pootakham, Sithichoke Tangphatsornruang, Anan Jongkaewwattana
African swine fever virus (ASFV) is a large, double-stranded DNA virus that causes a fatal, contagious disease specifically in pigs. However, prevention and control of ASFV outbreaks have been hampered by the lack of an effective vaccine or antiviral treatment for ASFV. Although ASFV has been reported to adapt to a variety of continuous cell lines, the phenotypic and genetic changes associated with
-
Molecular characterization of the integrative and conjugative elements harbouring multidrug resistance genes in Glaesserella parasuis Vet. Microbiol. (IF 3.3) Pub Date : 2024-02-04 Yongliang Che, Renjie Wu, Hongjie Li, Longbai Wang, Xuemin Wu, Qiuyong Chen, Rujing Chen, Lunjiang Zhou
It is widely known that integrative and conjugative elements (ICEs) play an important role in the transmission of resistance genes and other exogenous genes. The present study aimed to characterize the three novel ICEs including ICE, ICE and ICE, from . The ICEs from strains d76, Z44, and XP11 were predicted and identified by whole-genome sequencing (WGS) analysis, ICEfinder, and PCR. Characterization
-
Blockade of BTLA alone or in combination with PD-1 restores the activation and proliferation of CD8+ T cells during in vitro infection with NCP BVDV Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-24 Yu Liu, Zhibo Zhao, Siyu Su, Yang Li, Nannan Chen, Linru He, Meiqi Dong, Bin Xu, Zecai Zhang, Yulong Zhou, Zhanbo Zhu
Bovine viral diarrhea virus (BVDV) infection can result in typical peripheral blood lymphopenia and immune dysfunction. However, the molecular mechanism underlying the onset of lymphopenia remains unclear. B and T lymphocyte attenuator (BTLA) is a novel immune checkpoint molecule that primarily inhibits activation and proliferation of T cells. Blockade of BTLA with antibodies can boost the proliferation
-
Dynamics of antibody response and bacterial shedding of Mycoplasma hyorhinis and M. hyosynoviae in oral fluids from experimentally inoculated pigs Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-24 Precy D. Magtoto, Bailey L. Arruda, Ronaldo L. Magtoto, Juan Carlos Mora-Díaz, Rina B. Opulencia, David H. Baum, Jeff J. Zimmerman, Luis G. Giménez-Lirola
Mycoplasma hyorhinis (Mhr) and M. hyosynoviae (Mhs) are commensal organisms of the upper respiratory tract and tonsils but may also cause arthritis in pigs. In this study, 8-week-old cesarean-derived colostrum-deprived (CDCD) pigs (n = 30; 3 groups, 10 pigs per group, 2 pigs per pen) were inoculated with Mhr, Mhs, or mock-inoculated with culture medium and then pen-based oral fluids were collected
-
Live attenuated Mycobacterium bovis strains combined with the encapsulated H65 antigen as a vaccine strategy against bovine tuberculosis in a mouse model Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-26 Renée Onnainty, María Rocío Marini, María José Gravisaco, Elizabeth Andrea García, Clauss Aagaard, Ana Canal, Gladys Granero, Fabiana Bigi, Federico Carlos Blanco
s is an etiological agent of bovine tuberculosis (bTB) that also infects other mammals, including humans. The lack of an effective vaccine for the control of bTB highlights the need for developing new vaccines. In this study, we developed and evaluated an strain deleted in the virulence genes , and as a vaccine candidate against bTB in BALBc mice The evaluated strains were the new live vaccine and
-
GWAS and comparative genomics reveal candidate antibiotic resistance genes in the avian pathogen Gallibacterium anatis for six widespread antibiotics Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-26 Nicola Palmieri, Claudia Hess, Michael Hess
Gallibacterium anatis is a Gram-negative bacterium found in the respiratory and genital tracts of various animals, primarily poultry. Its association with septicemia and high mortality in poultry, along with the rise in multidrug-resistant strains, has amplified concerns. Recent research uncovered significant variability in antibiotic resistance profiles among G. anatis isolates from different Austrian
-
S2 subunit plays a critical role in pathogenesis of TW-like avian coronavirus infectious bronchitis virus Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-24 Gang Xu, Yuping Deng, Yang Li, Zonghui Zuo, Daowen Li, Shuhui Ma
To investigate the critical role of the S gene in determining pathogenesis of TW-like avian infectious bronchitis virus (IBV), we generated two recombinant IBVs (rGDaGD-S1 and rGDaGD-S2) by replacing either the S1 or S2 region of GD strain with the corresponding regions from an attenuated vaccine candidate aGD strain. The virulence and pathogenicity of these recombinant viruses was assessed both in
-
Mycoplasma mycoides subspecies capri, an uncommon mastitis and respiratory pathogen isolated in a German flock of goats Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-24 Henrik Wagner, Martin Heller, Ahmad Fawzy, Christiane Schnee, Anne Nesseler, Ute Kaim, Christa Ewers, Torsten Semmler, Joachim Spergser, Tilman Schultze, Tobias Eisenberg
Mycoplasma mycoides ssp. capri (Mmc) is one of the etiological microorganisms of contagious agalactia, which is among the diseases causing the highest economical losses in small ruminants. We report a disease outbreak in a German flock that led to significant suffering of goats characterized by mastitis, arthritis, pleuropneumonia and sudden deaths. Mmc was persistently isolated from many animals both
-
The construction and immunogenicity analyses of a recombinant pseudorabies virus with Senecavirus A VP3 protein co-expression Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-24 Qian Tao, Lei Xu, Yang Zhang, Yanting Yang, Zheyan Liu, Tong Xu, Siyuan Lai, Yanru Ai, Ling Zhu, Zhiwen Xu
Senecavirus A (SVA)-associated porcine idiopathic vesicular disease (PIVD) and Pseudorabies (PR) are highly contagious swine disease that pose a significant threat to the global pig industry. In the absence of an effective commercial vaccine, outbreaks caused by SVA have occurred in many parts of the world. In this study, the PRV variant strain PRV-XJ was used as the parental strain to construct a
-
Enhancing humoral and mucosal immune response of PED vaccine candidate by fusing S1 protein to nanoparticle multimerization Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-19 Minghui Li, Xueke Sun, Yilan Chen, Siqiao Wang, Qin Li, Yanan Wang, Yue Wang, Ruiqi Li, Peiyang Ding, Gaiping Zhang
Porcine epidemic diarrhea virus (PEDV) is a highly infectious pathogen with a high mortality rate, which poses a serious threat to newborn piglets. A rapid, safe and effective vaccine is necessary for protecting pigs from PED infection. Nanoparticles have become molecular scaffolds for displaying soluble antigens due to their unique physical and chemical properties. Here, a vaccine candidate was based
-
Experimental infection of goats with Mycobacterium microti induces subclinical pulmonary tuberculosis and mild responses to tuberculin skin tests Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-22 Cristian Melgarejo, Alex Cobos, Mariano Domingo, Guillermo Cantero, Xavier Moll, Iker A. Sevilla, Joseba M. Garrido, Lorraine Michelet, Maria Laura Boschiroli, Enric Vidal, Bernat Pérez de Val
Mycobacterium microti is a member of the Mycobacterium tuberculosis complex that seldom causes disease in livestock and humans. This study evaluated the effects on immunodiagnosis and the pathological findings in goats after experimental exposure by different routes and doses to M. microti. In a first experiment goats were challenged orally (PO, n = 7) or intranasally (IN, n = 7) with 104 CFU. In a
-
Rethinking the control of Streptococcus suis infection: Biofilm formation Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-21 Haikun Wang, Qingying Fan, Yuxin Wang, Li Yi, Yang Wang
Streptococcus suis is an emerging zoonotic pathogen that is widespread in swine populations. The control of S. suis infection and its associated diseases is a daunting challenge worldwide. Biofilm formation appears to be the main reason for the persistence of S. suis. In this review we gather existing knowledge on S. suis biofilm, describing the role of biofilm formation in S. suis virulence and drug
-
African swine fever virus maintains de novo global cellular protein synthesis and inhibits stress granules formation via dephosphorylating eIF2α Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-17 Han Gao, Xiaopeng Gao, Xing Liu, Yizhuo Luo, Jianhao Zhong, Jing Liu, Luling Yan, Heng Wang, Lang Gong, Guihong Zhang, Zezhong Zheng, Yankuo Sun
African swine fever virus (ASFV) has caused enormous economic losses since its first reported detection, and there is still no effective vaccines or drug treatment. During infection, viruses may employ various strategies, such as regulating the host endoplasmic reticulum stress/unfolded protein response or the formation of stress granules (SGs), to form an optimal environment for virus replication
-
Pathogenicity and horizontal transmission evaluation of a novel isolated African swine fever virus strain with a three-large-fragment-gene deletion Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-20 Han Gao, Dongdong Di, Qianwen Wu, Jie Li, Xing Liu, Zhiying Xu, Sijia Xu, Chengyu Wu, Lang Gong, Yankuo Sun, Guihong Zhang, Hongjun Chen, Heng Wang
African swine fever has caused substantial economic losses to China`s pig industry in recent years. Currently, the highly pathogenic African swine fever virus strain of genotype II is predominantly circulating in China, accompanied by a series of emerging isolates displaying unique genetic variations. The pathogenicity of these emerging strains is still unclear. Recently, a novel ASFV strain with a
-
The cAMP receptor protein gene contributes to growth, stress resistance, and colonization of Actinobacillus pleuropneumoniae Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-20 Qiyun He, Yaxuan Zheng, Kang Yan, Jia Tang, Fengming Yang, Yanhong Tian, Lijun Yang, Beibei Dou, Yunpeng Chen, Jun Gu, Huanchun Chen, Fangyan Yuan, Weicheng Bei
Porcine infectious pleuropneumonia (PCP) is a severe disease of porcine caused by Actinobacillus pleuropneumoniae (APP). The spread of PCP remains a threat to the porcine farms and has been known to cause severe economic losses. The cAMP receptor protein (CRP) serves as a pivotal player in helping bacteria adapt to shifts in their environment, particularly when facing the challenges posed by bacterial
-
Inhibition of pseudorabies virus replication via upregulated interferon response by targeting 7-dehydrocholesterol reductase Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-19 Zicheng Ma, Lei Guo, Mengjiao Pan, Chenlong Jiang, Depeng Liu, Yanni Gao, Juan Bai, Ping Jiang, Xing Liu
Pseudorabies virus (PRV) is an alpha-herpesvirus capable of infecting a range of animal species, particularly its natural host, pigs, resulting in substantial economic losses for the swine industry. Recent research has shed light on the significant role of cholesterol metabolism in the replication of various viruses. However, the specific role of cholesterol metabolism in PRV infection remains unknown
-
Multilocus sequence typing schemes for the emerging swine pathogen Mycoplasma hyosynoviae Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-15 Moritz Bünger, Michael Blümlinger, Igor Loncaric, Adriana Cabal Rosel, Werner Ruppitsch, Klaus Teich, Anna Kübber-Heiss, Isabel Hennig-Pauka, Andrea Ladinig, Joachim Spergser
Mycoplasma (M.) hyosynoviae is a commensal of the upper respiratory tract in swine, which has the potential to spread systemically, usually resulting in arthritis in fattening pigs and gilts. To date, very little is known about the epidemiology of M. hyosynoviae, mainly due to a lack of suitable typing methods. Therefore, this study aimed to develop both a conventional multi locus sequence typing (MLST)
-
Comment on “Prevalence and antimicrobial susceptibility of Mycoplasma bovis from the upper and lower respiratory tracts of healthy feedlot cattle and those diagnosed with bovine respiratory disease” Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-18 Peter W. Bergholz, Robin Temmerman, Daniel W. Scruggs, Michael T. Sweeney, Jeffrey L. Watts
Abstract not available
-
Exposure to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the endangered Iberian lynx (Lynx pardinus) Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-17 Javier Caballero Gómez, David Cano-Terriza, Joaquim Segalés, Júlia Vergara-Alert, Irene Zorrilla, Teresa del Rey, Jorge Paniagua, Moisés Gonzálvez, Leira Fernández-Bastit, Fernando Nájera, Juan I. Montoya-Oliver, Javier Salcedo, Ignacio García-Bocanegra
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an emerging zoonotic virus of public and animal health concern, of which felids have been suggested as potential reservoirs. Although SARS-CoV-2 exposure has been detected in domestic and wild captive animals belonging to Felidae family, surveillance has not been carried out in free-ranging wild felids so far. The aim of the present
-
Pasteurella multocida ST20 is widespread in Australian poultry farms and may infect wild waterbirds Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-12 Joanne L. Allen, Rhys N. Bushell, Amir H. Noormohammadi, Andrew W. Stent, Pam Whiteley, Glenn F. Browning, Marc S. Marenda
The bacterial agent that causes fowl cholera, Pasteurella multocida, was isolated from two deceased wild waterbirds in Victoria, Australia, in 2013. Whole genome sequence analysis placed the isolates into ST20, a subtype described in farmed chickens from Queensland, Australia and more recently in feedlot cattle and in pigs across a broader area of the continent. This study also found ST20 between 2009
-
Natural compound Sanggenon C inhibits porcine reproductive and respiratory syndrome virus replication in piglets Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-14 Xiao Liu, Yinan Meng, Jianyu He, Xuelian Jiang, Shuangquan Zhang, Dan Wang, Yanan Zhu, Zifang Zheng, Yunpeng Fan, Yupeng Yin, Shuqi Xiao
Porcine reproductive and respiratory syndrome virus is one of the main pathogens threatening the global pig industry, and there is still a lack of effective therapeutic drugs. Sanggenon C is a flavanone Diels-Alder adduct compound extracted from the root bark of the mulberry genus, which has blood pressure-reducing, anti-atherosclerotic, anti-oxidative, and anti-inflammatory effects. In our previous
-
Comparison of bovine viral diarrhea virus detection methods: Results of an international proficiency trial Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-11 Kerstin Wernike, Martin Beer
Bovine viral diarrhea virus (BVDV), one of the most important infectious cattle diseases globally, is being combated in multiple countries. The main source for virus transmission within herds and especially to unaffected cattle farms are life-long persistently infected (PI), immunotolerant animals. Therefore, the early identification of PI calves is a major pillar of disease control programs. In addition
-
Molecular characterization of antimicrobial resistance in Brachyspira species isolated from UK chickens: Identification of novel variants of pleuromutilin and beta-lactam resistance genes Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-15 Emma Stubberfield, Manal AbuOun, Roderick M. Card, David Welchman, Muna F. Anjum
Brachyspira species are Gram negative, anaerobic bacteria that colonise the gut of many animals, including poultry. In poultry, Brachyspira species can be commensal (B. innocens, B. murdochii, ‘B. pulli’) or pathogenic (B. pilosicoli, B. intermedia, B. alvinipulli or rarely B. hyodysenteriae), the latter causing avian intestinal spirochaetosis (AIS). Antimicrobial therapy options for treatment is limited
-
Prevalence and genetic characteristics of Salmonella enterica serovar Meleagridis from animals and humans Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-15 Kai Zhang, Zhenyu Wang, Pengyu Wang, Haiyan Xu, Xinan Jiao, Qiuchun Li
Salmonella enterica serovar Meleagridis (S. Meleagridis) is a non-typhoidal Salmonella serotype commonly found in food and humans. In this study, we investigated 61 Chinese S. Meleagridis isolates from various sources, predominantly from pigs and pig products. Additionally, the serotype was also identified in samples from human infections. Whole-genome sequencing analysis of these isolates, combined
-
Estimation of epidemiological cut-off values for eight antibiotics used for treatment of bovine mastitis caused by Streptococcus uberis and Streptococcus dysgalactiae subsp. dysgalactiae Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-15 Vibeke Frøkjær Jensen, Peter Damborg, Madelaine Norström, Bettina Nonnemann, Jannice Schau Slettemeås, Marit Smistad, Liv Sølverød, John Turnidge, Anne Margrete Urdahl, Kees Veldman, Alieda van Essen-Zandbergen, Lærke Boye Astrup
Interpretive criteria for antimicrobial susceptibility testing are lacking for most antimicrobials used for bovine streptococcal mastitis. The objectives of this study were to determine (tentative) epidemiological cut-off ((T)ECOFF) values for clinically relevant antibiotics used for treatment of bovine mastitis, and to estimate the proportion of acquired resistance (non-wild-types) in Streptococcus
-
ADP-ribosylation factor 6 promotes infectious bursal disease virus replication by affecting the internalization process via clathrin Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-14 Xinxin Zhang, Pei Gao, Li Wang, Liu Liu, Qiuxia Wang, Zhiyong Xu, Yanhong Zhang, Yan Yu, Jinyou Ma
ADP-ribosylation factor 6 (ARF6) is a small G protein with extensive functions, including regulation of cellular membrane transport and viral infection. Infectious bursal disease (IBD) is caused by infectious bursal disease virus (IBDV), which mainly invades the bursa of Fabricius and causes low immunity in poultry. Our study demonstrated that IBDV infection could promote the expression of ARF6; however
-
MicroRNA-194-5p/Heparin-binding EGF-like growth factor signaling mediates dexamethasone-induced activation of pseudorabies virus in rat pheochromocytoma cells Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-11 Chen Zhang, Yuxuan Liu, Fan Yang, Yifan Liu, Naixiu Wang, Yuhang Li, Yanqing Liu, Zhiyun Qiu, Lin Zhang, Xiaoyan You, Ling Gan
Pseudorabies virus (PRV) is a neurotropic virus, which infects a wide range of mammals. The activity of PRV is gradually suppressed in hosts that have tolerated the primary infection. Increased glucocorticoid levels resulting from stressful stimuli overcome repression of PRV activity. However, the host cell mechanism involved in the activation processes under stressful conditions remains unclear. In
-
2A2 protein of DHAV-1 induces duck embryo fibroblasts gasdermin E-mediated pyroptosis Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-10 Jingyu Wang, Hui Yan, Lei Bei, Shijin Jiang, Ruihua Zhang
The duck hepatitis A virus type 1 (DHAV-1) causes rapid death in ducklings by triggering a severe cytokine storm. Pyroptosis is an inflammatory form of programmed cell death that is directly related to an increase in pro-inflammatory cytokine levels. Only a few studies have explored the mechanisms underlying pyroptosis in virus-infected avian cells. In this study, we established an avian infection
-
Mitochondrial protein CHCHD10 inhibits NDV replication and reduces pathological changes Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-10 Xibing Yu, Hexiang Jiang, Jindou Li, Jiaxin Ding, Tong Wu, Kainan Chen, Zhuang Ding, Xiaohong Xu
Newcastle disease (ND) is a disease that threatens the world's poultry industry, which is caused by virulent Newcastle disease virus (NDV). As its pathogenic mechanism remains not fully clear, the proteomics of NDV-infected cells were analyzed. The results revealed that coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10) protein displayed a significant decrease at the late stage of NDV
-
-
Bovine parainfluenza type 3 virus induces incomplete autophagy to promote viral replication by activated beclin1 in vitro Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-03 Yu Han, Chongyang Wang, Kejia Lu, Xiaoyu Dong, Zhengwu Chang, Riteng Zhang, Qili Hou, Xinglong Wang, Sa Xiao, Haijin Liu, Zengqi Yang
Bovine Parainfluenza virus Type 3 (BPIV3) is one of the most important pathogens in cattle, capable of causing severe respiratory symptoms. Numerous studies have shown that autophagy plays a diverse role in the infection process of various pathogens. The influence of autophagy machinery on BPIV3 infection has not yet been confirmed. In the present study, we initially demonstrated that the expression
-
Cellular nuclear-localized U2AF2 protein is hijacked by the flavivirus 3’UTR for viral replication complex formation and RNA synthesis Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-03 Honggen Yuan, Jia hui Zou, Yun Luo, Jinhua Zhang, Hong Pan, Shengbo Cao, Huanchun Chen, Yunfeng Song
-
Construction and immunogenicity of Senecavirus A Virus-Like Particle Vaccine with adjuvant Vet. Microbiol. (IF 3.3) Pub Date : 2023-12-30 Jinyong Zhang, Peng Wang, Zhuoxin Li, Yubiao Xie, Ningyi Jin, Jicheng Han, He Zhang, Huijun Lu
Senecavirus A (SVA) is constantly associated with vesicular disease in pigs, and the clinical symptoms of pig infection with SVA are indistinguishable from other porcine vesicular diseases. Vaccine is one of the best methods to eliminate and control the spread of SVA. Virus-like particles (VLPs) can play important roles in prevention for infectious diseases. Here, the SVA VLPs was assembled by the
-
A potential dual protection vaccine: Recombinant feline herpesvirus-1 expressing feline parvovirus VP2 antigen Vet. Microbiol. (IF 3.3) Pub Date : 2024-01-02 Mengfang Yang, Yuzhou Jiao, Lisha Li, Yuanyuan Yan, Zhen Fu, Zirui Liu, Xiaoshuai Hu, Mengxia Li, Yuejun Shi, Junwei He, Zhou Shen, Guiqing Peng
Recently, herpesvirus viral vectors that stimulate strong humoral and cellular immunity have been demonstrated to be the most promising platforms for the development of multivalent vaccines, because they contain various nonessential genes and exhibit long-life latency characteristics. Previously, we showed that the feline herpesvirus-1 (FHV-1) mutant WH2020-ΔTK/gI/gE, which was safe for felines and
-
Isolation and identification of a duck-derived Japanese encephalitis virus and evaluation of its virulence in mice Vet. Microbiol. (IF 3.3) Pub Date : 2023-12-30 Chenxi Li, Linjie Zhang, Chenyang Tang, Yanbing Zhang, Wen Zhao
Birds including domestic and wild birds, as the amplifying or reservoir hosts of JEV, were sensitive to JEV infection and could develop a sufficiently high viremia to infect mosquitoes. However, most of JEV positive reports in birds were based on molecular detection, with few viruses isolated from clinical cases. In this study, one JEV strain, designated duck/2022-SD-1, was first isolated and identified