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Transcriptome Analysis Reveals Involvement of Oxidative Stress Response in a Copper-tolerant Fusarium oxysporum Strain Fungal Biol. (IF 2.789) Pub Date : 2021-01-11 Lorenz Rhuel P. Ragasa; Santiago Emil A. Joson; Windy Lou R. Bagay; Teresita R. Perez; Michael C. Velarde
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Unidirectional mating-type switching confers self-fertility to Thielaviopsis cerberus, the only homothallic species in the genus Fungal Biol. (IF 2.789) Pub Date : 2021-01-07 Daniella Krämer; Frances A. Lane; Emma T. Steenkamp; Brenda D. Wingfield; P. Markus Wilken
Sexual reproduction is ubiquitous in nature, and nowhere is this more so than in the fungi. Heterothallic behaviour is observed when there is a strict requirement of contact between two individuals of opposite mating-type for sexual reproduction to occur. In contrast, a homothallic species can complete the entire sexual cycle in isolation, although several genetic mechanisms underpin this self-fertility
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Distribution of a novel enzyme of sialidase family among native filamentous fungi Fungal Biol. (IF 2.789) Pub Date : 2021-01-05 Radoslav Abrashev; Ekaterina Krumova; Penka Petrova; Rumyana Eneva; Nedelina Kostadinova; Jeni Miteva-Staleva; Stephan Engibarov; Galina Stoyancheva; Yana Gocheva; Vera Kolyovska; Vladislava Dishliyska; Boryana Spassova; Maria Angelova
Sialidases (neuraminidases, EC 3.2.1.18) are widely distributed in biological systems such as viruses, bacteria, protozoa, as well as in the deuterostome lineage of animals. But there are only scarce data on the sialidase production by filamentous fungi, especially isolated from native sources. The aim of this study was to obtain information about sialidase distribution in filamentous fungi from non-clinical
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Analysis of tRNA-derived RNA fragments (tRFs) in Cryptococcus spp.: RNAi-independent generation and possible compensatory effects in a RNAi-deficient genotype Fungal Biol. (IF 2.789) Pub Date : 2021-01-04 Rodrigo Silva Araujo Streit; Patrícia Aline Gröhns Ferrareze; Marilene Henning Vainstein; Charley Christian Staats
Small RNAs (sRNAs) are key factors in the regulation of gene expression. Recently, a new class of regulatory sRNAs derived from tRNAs was described, the tRNA-derived RNA fragments (tRFs). Such RNAs range in length from 14 to 30 nucleotides and are produced from both mature and primary tRNA transcripts, with very specific cleavage sites along the tRNA sequence. Although several mechanisms have been
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Carotenoid biosynthesis and the evolution of carotenogenesis genes in rust fungi Fungal Biol. (IF 2.789) Pub Date : 2020-12-31 Erpei Wang; Chongmei Dong; Peng Zhang; Thomas H. Roberts; Robert F. Park
Diseases caused by rust fungi pose a significant threat to global plant production. Although carotenoid pigments are produced in spores of nearly all rust species, the corresponding biosynthesis pathway(s) have not been investigated. Here, candidate genes for carotenoid biosynthesis in Puccinia graminis f. sp. tritici (Pgt) were identified, cloned and functionally complemented using specifically engineered
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The serine peptidase inhibitor N-ρ-tosyl-L-phenylalanine chloromethyl ketone (TPCK) affects the cell biology of Candida haemulonii species complex Fungal Biol. (IF 2.789) Pub Date : 2020-12-23 Xênia Macedo Souto; Lívia de Souza Ramos; Simone Santiago Carvalho de Oliveira; Marta Helena Branquinha; André Luis Souza dos Santos
Candida haemulonii species complex (Candida haemulonii, Candida haemulonii var. vulnera and Candida duobushaemulonii) is composed by emerging and multidrug-resistant (MDR) yeasts. Candidiasis, the disease caused by these species, is difficult to treat and culminates in clinical failures and patient death. It is well-known that Candida peptidases play important roles in the fungus-host interactions
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Enhancement of exopolysaccharide production from Ganoderma lucidum using a novel submerged volatile co-culture system Fungal Biol. (IF 2.789) Pub Date : 2020-10-09 Fatemeh Asadi; Mohammad Barshan-tashnizi; Ashrafalsadat Hatamian-Zarmi; Fatemeh Davoodi-Dehaghani; Bahman Ebrahimi-Hosseinzadeh
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Metaproteomics reveals the structural and functional diversity of Dermatocarpon miniatum (L.) W. Mann. Microbiota Fungal Biol. (IF 2.789) Pub Date : 2020-10-09 Junaid Ullah; Zainab Khanum; Ishtiaq Ahmad Khan; Abdul Nasir Khalid; Syed Ghulam Musharraf; Arslan Ali
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Enhanced xylanase and endoglucanase production from Beauveria bassiana SAN01, an entomopathogenic fungal endophyte Fungal Biol. (IF 2.789) Pub Date : 2020-10-09 Ayodeji Amobonye; Prashant Bhagwat; Suren Singh; Santhosh Pillai
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Floral fungal-bacterial community structure and co-occurrence patterns in four sympatric island plant species Fungal Biol. (IF 2.789) Pub Date : 2020-10-15 Xin Qian; Xiaojuan Li; Hanzhou Li; Dianxiang Zhang
Flowers’ fungal and bacterial communities can exert great impacts on host plant wellness and reproductive success—both directly and indirectly through species interactions. However, information about community structure and co-occurrence patterns in floral microbiome remains scarce. Here, using culture-independent methods, we investigated fungal and bacterial communities associated with stamens and
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Dynamics of solute/matric stress interactions with climate change abiotic factors on growth, gene expression and ochratoxin A production by Penicillium verrucosum on a wheat-based matrix Fungal Biol. (IF 2.789) Pub Date : 2020-10-16 Shaimaa Abdelmohsen; Carol Verheecke-Vaessen; Esther Garcia-Cela; Angel Medina; Naresh Magan
Penicillium verrucosum contaminates temperate cereals with ochratoxin A (OTA) during harvesting and storage. We examined the effect of temperature (25 vs 30 oC), CO2 (400 vs 1000 ppm) and matric/solute stress (-2.8 vs -7.0 MPa) on (i) growth, (ii) key OTA biosynthetic genes and (iii) OTA production on a milled wheat substrate. Growth was generally faster under matric than solute stress at 25 oC, regardless
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A moderate level of hypovirulence conferred by a hypovirus in the avocado white root rot fungus, Rosellinia necatrix Fungal Biol. (IF 2.789) Pub Date : 2020-10-29 Juan M. Arjona-López; Paul Telengech; Nobuhiro Suzuki; Carlos J. López-Herrera
Two isolates of Rosellinia necatrix (Rn118-8 and Rn480) have previously obtained from diseased avocado trees in commercial orchards of the coastal area in southern Spain. Rn118-8 and Rn480 have weak virulence on avocado plants, and are infected by R. necatrix hypovirus 2 (RnHV2). In this work, the possible biological effects of the hypovirus on R. necatrix were tested. First, RnHV2 was transmitted
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Effect of extracellular polysaccharide production on the analysis of intracellular metabolites from Trichoderma harzianum grown under carbon-limited conditions Fungal Biol. (IF 2.789) Pub Date : 2020-12-11 Lucas Gelain; José Geraldo da Cruz Pradella; Aline Carvalho da Costa; Luuk van der Wielen; Walter M. van Gulik
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On the TPPP-like proteins of flagellated fungi Fungal Biol. (IF 2.789) Pub Date : 2020-12-11 Ferenc Orosz
TPPP-like proteins, exhibiting microtubule stabilizing function, constitute a eukaryotic protein superfamily, characterized by the presence of the p25alpha domain. TPPPs in the strict sense are present in animals except the placozoan Trichoplax adhaerens, which instead contains apicortin where a part of the p25alpha domain is combined with a DCX domain. Apicortin is absent in any other animal and occurs
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Cryphonectria carpinicola sp. nov. associated with hornbeam decline in Europe Fungal Biol. (IF 2.789) Pub Date : 2020-12-08 Carolina Cornejo; Andrea Hauser; Ludwig Beenken; Thomas Cech; Daniel Rigling
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Root fungal endophytes: identity, phylogeny and roles in plant tolerance to metal stress Fungal Biol. (IF 2.789) Pub Date : 2020-12-03 B.A.R.B.E.R.I.S. Louise; M.I.C.H.A.L.E.T. Serge; P.I.O.L.A. Florence; B.I.N.E.T. Philippe
Metal trace elements accumulate in soils mainly because of anthropic activities, leading living organisms to develop strategies to handle metal toxicity. Plants often associate with root endophytic fungi, including nonmycorrhizal fungi, and some of these organisms are associated with metal tolerance. The lack of synthetic analyses of plant-endophyte-metal tripartite systems and the scant consideration
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Requirement of LaeA for sporulation, pigmentation and secondary metabolism in Chaetomium globosum Fungal Biol. (IF 2.789) Pub Date : 2020-12-03 Ming Cheng; Shanshan Zhao; Congyu Lin; Jinzhu Song; Qian Yang
The global regulator LaeA has been confirmed to govern the production of secondary metabolites in fungi. Herein, we examined the role of LaeA in Chaetomium globosum. Similarly as observed in other filamentous, CgLaeA had a significant effect on the secondary metabolism. The ΔCglaeA mutant strain did not exhibit chaetoglobosin A, whereas its production was restored in the CglaeAC strain. In addition
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Aphanomyces astaci mtDNA: insights into the pathogen´s differentiation and its genetic diversity from other closely related oomycetes Fungal Biol. (IF 2.789) Pub Date : 2020-12-02 Gloria Casabella-Herrero; María Martínez-Ríos; Satu Viljamaa-Dirks; Laura Martín-Torrijos; Javier Diéguez-Uribeondo
The causative agent of crayfish plague, Aphanomyces astaci (Saprolegniales, Oomycota), is one of the 100 world’s worst invasive alien species and represents a major threat to freshwater crayfish species worldwide. A better understanding of the biology and epidemiology of A. astaci relies on the application of efficient tools to detect the pathogen and assess its genetic diversity. In this study, we
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Time-scale dynamics of proteome predicts the central carbon metabolism involved in triterpenoid accumulation responsive to nitrogen limitation in Ganoderma lucidum Fungal Biol. (IF 2.789) Pub Date : 2020-12-01 Danhong Lian; Lian Li; Xin Liu; Xin Zhong; Haizhen Wang; Sha Zhou; Li Gu
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Minimal nutrient requirements for induction of germination of Aspergillus niger conidia Fungal Biol. (IF 2.789) Pub Date : 2020-11-27 Maryam Ijadpanahsaravi; Maarten Punt; Han A.B. Wösten; Wieke R. Teertstra
Aspergillus niger reproduces asexually by forming conidia. Here, we studied the minimal nutrient requirements that activate germination of A. niger conidia. To this end, germination of conidia was monitored in time using an oCelloScope imager. Data was used as input in an asymmetric model to describe the process of swelling and germ tube formation. The estimated maximum number of spores (Pmax) that
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Successful induction and recognition of conidiation, conidial germination and chlamydospore formation in pure culture of Morchella Fungal Biol. (IF 2.789) Pub Date : 2020-11-27 Bin-Hong Yuan; Huan Li; Lu Liu; Xi-Hui Du
Morels, fungi from the genus Morchella, are popular edible mushrooms. However, little knowledge of their asexual reproduction and inaccessible pure mitospores hamper illumination of their life cycle. Herein, we successfully induced conidiation, conidial germination and chlamydospore formation in pure culture of Morchella sextelata. Conidiation proceeded via four morphologically distinct stages: development
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Functional analysis of a chaetoglobosin A biosynthetic regulator in Chaetomium globosum Fungal Biol. (IF 2.789) Pub Date : 2020-11-27 Ming Cheng; Shanshan Zhao; He Liu; Yutao Liu; Congyu Lin; Jinzhu Song; Chitti Thawai; Sittichai Charoensettasilp; Qian Yang
Cytochalasins are a group of fungal secondary metabolites with diverse structures and bioactivities, including chaetoglobosin A production. Chaetoglobosin A is produced by Chaetomium globosum and has potential antifungal activity. Bioinformatics analysis of the chaetoglobosin A gene cluster (che) showed it that consists of nine open reading frames, including those encoding polyketide synthases (PKSs)
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Novel Black Yeast-like Species in Chaetothyriales with Ant-associated Life Styles Fungal Biol. (IF 2.789) Pub Date : 2020-11-27 Yu Quan; Sarah A. Ahmed; Nickolas Menezes da Silva; Abdullah M.S. Al-Hatmi; Veronika E. Mayer; Shuwen Deng; Yingqian Kang; G. Sybren de Hoog; Dongmei Shi
Among ancestral fungi in Chaetothyriales, several groups have a life style in association with tropical ants, either in domatia or in carton-nests. In the present study, two strains collected from ant carton in Thailand and Malaysia were found to represent hitherto undescribed species. Morphological, physiological, phylogenetic data and basic genome information are provided for their classification
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Multigene phylogeny and taxonomic revision of Atheliales s.l.: reinstatement of three families and one new family, Lobuliciaceae fam. nov Fungal Biol. (IF 2.789) Pub Date : 2020-11-27 Bobby P. Sulistyo; Karl-Henrik Larsson; Danny Haelewaters; Martin Ryberg
Atheliales (Agaricomycetes, Basidiomycota) is an order mostly composed of corticioid fungi, containing roughly 100 described species in approximately 20 genera. Members exhibit remarkable ecological diversity, including saprotrophs, ectomycorrhizal symbionts, facultative parasites of plants or lichens, and symbionts of termites. Ectomycorrhizal members are well known because they often form a major
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Studies toward the comprehension of fungal-macroalgae interaction in cold marine regions from a biotechnological perspective Fungal Biol. (IF 2.789) Pub Date : 2020-11-19 M.M. Martorell; M. Lannert; C.V. Matula; M.L. Quartino; L.I.C. de Figueroa; WP Mac Cormack; L.A.M. Ruberto
In marine ecosystems, macroalgae are the habitat for several microorganisms, fungi being among them. In the Antarctic benthic coastal ecosystem, macroalgae play a key role in organic matter cycling. In this study, 13 different macroalgae from Potter Cove and surrounding areas were sampled and 48 fungal isolates were obtained from six species, four Rhodophyta Ballia callitricha, Gigartina skottsbergii
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Contrasting genetic structuring in the closely related basidiomycetes Trichaptum abietinum and T. fuscoviolaceum (Hymenochaetales) Fungal Biol. (IF 2.789) Pub Date : 2020-11-19 Kristian Skaven Seierstad; Renate Fossdal; Otto Miettinen; Tor Carlsen; Inger Skrede; Håvard Kauserud
Trichaptum abietinum and Trichaptum fuscoviolaceum (Hymenochaetales, Basidiomycota) are closely related saprotrophic fungi, widely distributed on coniferous wood in temperate regions worldwide. Three intersterility groups have previously been detected in T. abietinum, while no prezygotic barriers have been proven within T. fuscoviolaceum. The aim of this study was to reveal the phylogeography and genetic
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Saprolegnia parasitica zoospore activity and host survival indicates isolate variation in host preference Fungal Biol. (IF 2.789) Pub Date : 2020-11-19 Emily Matthews; Amy Ellison; Joanne Cable
The ubiquitous freshwater pathogen Saprolegnia parasitica has long been considered a true generalist, capable of infecting a wide range of fish species. It remains unclear, however, whether different isolates of this pathogen, obtained from distinct geographic locations and host species, display differences in host preference. To assess this, the current study examined the induced zoospore encystment
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Moniliophthora perniciosa, the mushroom causing witches’ broom disease of cacao: Insights into its taxonomy, ecology and host range in Brazil Fungal Biol. (IF 2.789) Pub Date : 2020-09-08 Daniela O. Lisboa; Harry C. Evans; João P.M. Araújo; Samuel G. Elias; Robert W. Barreto
Witches' broom caused by Moniliophthora perniciosa is the main disease of cacao (Theobroma cacao) in Brazil. The fungus is known to occur on other host families and these populations have been addressed in the literature as biotypes: C (Malvaceae); H (Malpighiaceae); L (Bignoniaceae) and S (Solanaceae). No complete elucidation of the phylogenetic relationships of isolates obtained from this disparate
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Molecular and genetic evidence for a tetrapolar mating system in Sparassis latifolia Fungal Biol. (IF 2.789) Pub Date : 2020-09-22 Yongqi Li; Ying Yang; Xianhua Huang; Jie Huang; Caihong Dong
Sparassis latifolia is a valuable edible fungus cultivated in East Asia that is rich in β-glucans. Understanding the mating system and sexual life cycle is important not only for breeding programs to improve strains but also for studies on speciation and population structures. In the present study, mating experiments using monokaryons derived from two different parental strains were performed. Chi-squared
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Growth induced translocation effectively directs an amino acid analogue to developing zones in Agaricus bisporus Fungal Biol. (IF 2.789) Pub Date : 2020-09-17 Koen C. Herman; Han A.B. Wösten; Mark D. Fricker; Robert-Jan Bleichrodt
The vegetative mycelium of Agaricus bisporus supplies developing white button mushrooms with water and nutrients. However, it is not yet known which part of the mycelium contributes to the feeding of the mushrooms and how this depends on growth conditions. Here we used photon counting scintillation imaging to track translocation of the 14C-radiolabeled metabolically inert amino acid analogue α-aminoisobutyric
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The chaperone Lhs1 contributes to the virulence of the fish-pathogenic oomycete Aphanomyces invadans Fungal Biol. (IF 2.789) Pub Date : 2020-09-17 Nurul Aqilah Iberahim; Neeraj Sood; Pravata Kumar Pradhan; Johannes van den Boom; Pieter van West; Franziska Trusch
Oomycetes are fungal-like eukaryotes and many of them are pathogens that threaten natural ecosystems and cause huge financial losses for the aqua- and agriculture industry. Amongst them, Aphanomyces invadans causes Epizootic Ulcerative Syndrome (EUS) in fish which can be responsible for up to 100% mortality in aquaculture. As other eukaryotic pathogens, in order to establish and promote an infection
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New molecular marker for phylogenetic reconstruction of black yeast-like fungi (Chaetothyriales) with hypothetical EIF2AK2 kinase gene Fungal Biol. (IF 2.789) Pub Date : 2020-09-24 Cláudia Barbosa Assunção; Edgar Lacerda de Aguiar; Abdullah M.S. Al-Hatmi; Vanessa Cristina Silva Vieira; Amanda Sanchez Machado; Cristina Junta; Sybren de Hoog; Rachel Basques Caligiorne
In eukaryotes, phosphorylation of the α-subunit of eIF2 is a mechanism to adjust cellular gene expression profiles in response to specific signals. The eIF2α kinases are a group of serine–threonine kinases that perform important functions in response to infection, proteotoxicity, and nutrient scavenging. The conserved nature of eIF2α kinases among fungi makes them potential evolutionary markers, which
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Differential responses of genes and enzymes associated with ROS protective responses in the sugarcane smut fungus Fungal Biol. (IF 2.789) Pub Date : 2020-10-04 L.P. Peters; N.S. Teixeira-Silva; A.P. Bini; M.M.L. Silva; N. Moraes; G.S. Crestana; S. Creste; R.A. Azevedo; G. Carvalho; C.B. Monteiro-Vitorello
The fungal pathogen Sporisorium scitamineum causes sugarcane smut disease. We have previously shown that resistant sugarcane plants induce ROS, coinciding with a delay in fungal colonization. Here, we investigated whether the fungus modifies the enzymatic antioxidant system in vitro and when colonizing sugarcane tissues in response to ROS. In vitro, the exposure to ROS did not affect cell integrity
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Roles of autophagy-related genes in conidiogenesis and blastospore formation, virulence, and stress response of Beauveria bassiana Fungal Biol. (IF 2.789) Pub Date : 2020-10-08 Jia Hou; Jia-Jia Wang; Hai-Yan Lin; Ming-Guang Feng; Sheng-Hua Ying
Autophagy is a conserved intracellular recycling mechanism, in which autophagy-related genes 12 and 16 (ATG12 and ATG16) function in a complex controlling the ubiquitin-like conjugation system. In the insect-pathogenic fungus Beauveria bassiana, ATG12 and ATG16 were functionally characterized. Disruption of BbATG12 or BbATG16 resulted in the absence of autophagic bodies under starvation stress. ΔBbATG12
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Penicillium rubens germination on desiccated and nutrient-depleted conditions depends on the water activity during sporogenesis Fungal Biol. (IF 2.789) Pub Date : 2020-10-21 Philip Ruijten; Hendrik P. Huinink; Olaf C.G. Adan
Fungal growth often appears in a surrounding where water and nutrients are scarce. The impact of this environment during sporogenesis on subsequent growth is often neglected. This study investigates the effect of water availability during sporogenesis on subsequent early growth. Therefore, a carbon-depleted substrate was constructed. Humidity is then the only parameter of interest. The water conditions
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Map-based cloning identifies velvet A as a critical component of virulence in Fusarium pseudograminearum during infection of wheat heads Fungal Biol. (IF 2.789) Pub Date : 2020-11-11 Donald M. Gardiner; Anca Rusu; Aurélie H. Benfield; Kemal Kazan
Although better known as a pathogen of wheat stem bases, Fusarium pseudograminearum also causes Fusarium head blight. A natural isolate of F. pseudograminearum was identified that showed severely reduced virulence towards wheat heads and a map-based cloning approach was undertaken to identify the genetic basis of this phenotype. Using a population of 95 individuals, a single locus on chromosome 1 was
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3-Hydroxytyrosol regulates biofilm growth in Cunninghamella elegans Fungal Biol. (IF 2.789) Pub Date : 2020-11-04 Mohd Faheem Khan; Cormac D. Murphy
In contrast to yeast biofilms, those of filamentous fungi are relatively poorly understood, in particular with respect to their regulation. Cunninghamella elegans is a filamentous fungus that is of biotechnological interest as it catabolises drugs and other xenobiotics in an analogous manner to animals; furthermore, it can grow as a biofilm enabling repeated batch biotransformations. Precisely how
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Carbamoyl phosphate synthetase subunit Cpa1 interacting with Dut1, controls development, arginine biosynthesis, and pathogenicity of Colletotrichum gloeosporioides Fungal Biol. (IF 2.789) Pub Date : 2020-11-02 Qingqun Tan; Xuanzhu Zhao; Haiyong He; Junxiang Zhang; Tuyong Yi
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Continuous production of enzymes under carbon-limited conditions by Trichoderma harzianum P49P11 Fungal Biol. (IF 2.789) Pub Date : 2020-10-28 Lucas Gelain; Esther Kingma; José Geraldo da Cruz Pradella; Aline Carvalho da Costa; Luuk van der Wielen; Walter M. van Gulik
Carbon-limited chemostat cultures were performed using different carbon sources (glucose, 10 and 20 g/L; sucrose, 10 g/L; fructose/glucose, 5.26/5.26 g/L; carboxymethyl cellulose, 10 g/L; and carboxymethyl cellulose/glucose, 5/5 g/L) to verify the capability of the wild type strain Trichoderma harzianum to produce extracellular enzymes. All chemostat cultures were carried out at a fixed dilution rate
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Secretomes of medically important fungi reflect morphological and phylogenetic diversity Fungal Biol. (IF 2.789) Pub Date : 2020-08-08 Sarai Varona; José L. Lavín; José A. Oguiza
Secretome represents a main target for understanding the mechanisms of fungal adaptation. In the present study, we focus on the secretomes of fungi associated with infections in humans and other mammals in order to explore relationships between the diverse morphological and phylogenetic groups. Almost all the mammalian pathogenic fungi analyzed have secretome sizes smaller than 1000 proteins and, secreted
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Smt3, a homologue of yeast SUMO, contributes to asexual development, environmental adaptation, and host infection of a filamentous entomopathogen Fungal Biol. (IF 2.789) Pub Date : 2020-08-08 Tong Liu; Xiaolin Meng; Jianwen Chen; Qian’an Fang; Jie Wang
Small ubiquitin-like modifiers (SUMOs) act as the modifiers that regulate several important eukaryotic cell events during sumoylation, but little is known about the functions of SUMO or sumoylation in filamentous entomopathogens. Here, we report the important roles of a single SUMO-encoding gene, smt3, in Beauveria bassiana, a filamentous fungal insect pathogen that serves as a main source of wide-spectrum
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Efficient expressions of reporter genes in the industrial filamentous fungus Sclerotium rolfsii mediated by Agrobacterium tumefaciens Fungal Biol. (IF 2.789) Pub Date : 2020-08-10 Meilin Li; Peng Chang; Xiaohong Pan; Tadayuki Imanaka; Yasuo Igarashi; Feng Luo
Sclerotium rolfsii (teleomorph Athelia rolfsii) is one of the plant pathogenic basidiomycetes, which causes severe stem-rot disease in hundreds of plants and produces important metabolites, such as scleroglucan and TF-specific lectin. However, further molecular biological research on this filamentous fungus is severely plateaued out due to the lack of genetic methods. In this study, the A. tumefaciens
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Diversity, prevalence and phylogenetic positioning of Botrytis species in Brazil Fungal Biol. (IF 2.789) Pub Date : 2020-08-24 Daiana M.Q. Azevedo; Sarah D.S. Martins; Débora C. Guterres; Mateus D. Martins; Leonardo Araújo; Lúcio M.S. Guimarães; Acelino C. Alfenas; Gleiber Q. Furtado
Botrytis is a necrotrophic fungal genus of great economic importance worldwide. Together, the Botrytis species are able to infect over one thousand host plant species, including dicotyledons and monocotyledons. As the identification of Botrytis species in Brazil has mostly been based only on morphological characterization and comparisons of the rDNA ITS region, which is not informative in the genus
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Evidence of fungal decay in petrified legume wood from the Neogene of the Bengal Basin, India Fungal Biol. (IF 2.789) Pub Date : 2020-08-22 Anwesha Biswas; Meghma Bera; Mahasin Ali Khan; R.A. Spicer; T.E.V. Spicer; Krishnendu Acharya; Subir Bera
Silicified fossil legume woods of Cynometroxylon Chowdhury & Ghosh collected from the Neogene (late Miocene) sediments of the Bengal Basin, eastern India, exhibit fungal decay seldom found in the fossil record. The wood possesses numerous perforate areas on the surface that seem to be the result of extensive fungal activity. In transverse section, the decayed areas (pockets) appear irregular to ellipsoidal
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The ADP-ribosylation factor-like small GTPase FgArl1 participates in growth, pathogenicity and DON production in Fusarium graminearum Fungal Biol. (IF 2.789) Pub Date : 2020-08-29 Chenyu Wang; Yixiao Wang; Yao Wang; Zuodong Wang; Liyuan Zhang; Yuancun Liang; Lei Chen; Shenshen Zou; Hansong Dong
Fusarium graminearum is the main pathogen of Fusarium head blight (FHB) in wheat and related species, which causes serious production decreases and economic losses and produces toxins such as deoxynivalenol (DON), which endangers the health of humans and livestock. Vesicle transport is a basic physiological process required for cell survival in eukaryotes. Many regulators of vesicle transport are reported
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The fungal community associated with the ambrosia beetle Xylosandrus compactus invading the mediterranean maquis in central Italy reveals high biodiversity and suggests environmental acquisitions Fungal Biol. (IF 2.789) Pub Date : 2020-10-03 Carmen Morales-Rodríguez; Ivano Sferrazza; Maria Pia Aleandri; Matteo Dalla Valle; Stefano Speranza; Mario Contarini; Andrea Vannini
In summer 2016 a severe infestation of the alien ambrosia beetle Xylosandrus compactus was recorded from the Mediterranean maquis in the Circeo National Park in Central Italy. Trees and shrubs were infested and displayed wilting and necrosis of terminal branches caused by the combined impact of the insect and associated pathogenic fungi. A preliminary screening carried out on captured adults resulted
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Penicillium oxalicum S-adenosylmethionine synthetase is essential for the viability of fungal cells and the expression of genes encoding cellulolytic enzymes Fungal Biol. (IF 2.789) Pub Date : 2020-09-21 Yueyan Hu; Kaili Zhao; Yinbo Qu; Xin Song; Jian Zhao; Yuqi Qin
As the universal methyl donor for methylation reactions, S-adenosylmethionine (AdoMet) plays an indispensable role in most cellular metabolic processes. AdoMet is synthesized by AdoMet synthetase. We identified the only one AdoMet synthetase (PoSasA) in filamentous fungus Penicillium oxalicum. PoSasA was widely distributed in mycelium at different growth stages. The absence of PoSasA was lethal for
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Chemo-profiling of bioactive metabolites from Chaetomium globosum for biocontrol of Sclerotinia rot and plant growth promotion Fungal Biol. (IF 2.789) Pub Date : 2020-08-08 Rakesh Kumar; Aditi Kundu; Anirban Dutta; Supradip Saha; Amrita Das; Arpan Bhowmik
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Fossil callimothalloid fungi: Revised taxonomy, modern equivalents and palaeoecology. Fungal Biol. (IF 2.789) Pub Date : 2020-08-05 Grzegorz Worobiec,Elżbieta Worobiec,Boglárka Erdei
This paper presents the reinterpretation of the taxonomic position of fossil epiphyllous callimothalloid fungi belonging to the fossil-genera Callimothallus (hitherto classified to the family Microthyriaceae) and Cribrites (?Microthyriales). These fungi thrived from the Late Cretaceous up to the Holocene. Investigation are based on collected material from the Oligocene of Hungary and the Miocene of
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Low genetic differentiation between apotheciate Usnea florida and sorediate Usnea subfloridana (Parmeliaceae, Ascomycota) based on microsatellite data. Fungal Biol. (IF 2.789) Pub Date : 2020-07-31 Polina Degtjarenko,Kristiina Mark,Rolands Moisejevs,Dmitry Himelbrant,Irina Stepanchikova,Andrei Tsurykau,Tiina Randlane,Christoph Scheidegger
Accurate species delimitation has a pivotal role in conservation biology, and it is especially important for threatened species where decisions have political and economic consequences. Finding and applying appropriate character sets and analytical tools to resolve interspecific relationships remains challenging in lichenized fungi. The main aim of our study was to re-assess the species boundaries
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Functional trade-off of hydration strategies in old forest epiphytic cephalolichens. Fungal Biol. (IF 2.789) Pub Date : 2020-07-30 Annie B Ås Hovind,Nathan H Phinney,Yngvar Gauslaa
Although water is essential for photosynthetic activation in lichens, rates of vapor uptake and activation in humid air, which likely influence their niche preferences and distribution ranges, are insufficiently known. This study simultaneously quantifies rehydration kinetics and PSII reactivation in sympatric, yet morphologically and functionally distinct cephalolichens (Lobaria amplissima, Lobaria
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Molecular analysis of mating type loci from the mycophenolic acid producer Penicillium brevicompactum: Phylogeny and MAT protein characterization suggest a cryptic sexual life cycle. Fungal Biol. (IF 2.789) Pub Date : 2020-07-29 Yasaman Mahmoudjanlou,Tim A Dahlmann,Ulrich Kück
The mycophenolic acid producing ascomycete Penicillium brevicompactum is considered to be an anamorphic (asexual) species, for which a sexual cycle was never observed. However, since recent reports of otherwise asexually propagating filamentous fungi have demonstrated a sexual cycle controlled by mating type loci, we carried out a molecular analysis of mating type loci from P. brevicompactum. Using
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Strong preference for the integration of transforming DNA via homologous recombination in Trichoderma atroviride. Fungal Biol. (IF 2.789) Pub Date : 2020-07-29 Edgardo Ulises Esquivel-Naranjo,Alfredo Herrera-Estrella
Trichoderma species play important roles in nature as plant growth promotors and antagonists of phytopathogenic fungi, and are used as models to study photomorphogenesis. Molecular tools have been implemented to manipulate and improve these fungi. However, instability of transformants or very low frequency of homologous recombination has been reported. Here, we report the fate of transforming DNA,
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Osmolytes and membrane lipids in the adaptation of micromycete Emericellopsis alkalina to ambient pH and sodium chloride. Fungal Biol. (IF 2.789) Pub Date : 2020-07-29 Olga A Danilova,Elena A Ianutsevich,Sofiya A Bondarenko,Marina L Georgieva,Daria A Vikchizhanina,Natalia V Groza,Elena N Bilanenko,Vera M Tereshina
The accumulation of low molecular weight cytoprotective compounds (osmolytes) and changes in the membrane lipids composition are of key importance for the adaptation to stress impacts. However, the reason behind the wide variety of osmolytes present in the cell remains unclear. We suggest that specific functions of osmolytes can be revealed by studying the adaptation mechanisms of the mycelial fungus
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Endophytic fungi, host genotype, and their interaction influence the growth and production of key chemical components of Dendrobium catenatum. Fungal Biol. (IF 2.789) Pub Date : 2020-07-29 Ling-Shang Wu,Wei-Guo Dong,Jin-Ping Si,Jing-Jing Liu,Yu-Qiu Zhu
To examine how host plant genotype, endophytic fungal species, and their interaction may affect growth and key chemical content and composition in an important orchid species, we assessed four Dendrobium catenatum cultivars co-cultured with three fungi previously isolated from D. catenatum. Fungal endophytes (Tulasnella sp., Leptosphaeria microscopica, and Guignardia sp.) specifically affected the
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Localization of the insect pathogenic fungal plant symbionts Metarhizium robertsii and Metarhizium brunneum in bean and corn roots. Fungal Biol. (IF 2.789) Pub Date : 2020-07-28 Sarah Lahey,Steven Angelone,Marie Olivia DeBartolo,Caio Coutinho-Rodrigues,Michael J Bidochka
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The mitochondrial genome contribution to the phylogeny and identification of Metarhizium species and strains. Fungal Biol. (IF 2.789) Pub Date : 2020-07-27 Alexandra M Kortsinoglou,Zack Saud,Dan C Eastwood,Tariq M Butt,Vassili N Kouvelis
The genus Metarhizium is composed of entomopathogenic fungal biological control agents (BCAs) used for invertebrate pest control. The phylogenetic relationships of species within this genus are still under scrutiny as several cryptic species can be found. In this work, the mitochondrial (mt) genome of Metarhizium brunneum ARSEF 4556 was fully sequenced and a comparative genome analysis was conducted
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Characterization and functional analysis of ERAD-related AAA+ ATPase Cdc48 in Aspergillus oryzae. Fungal Biol. (IF 2.789) Pub Date : 2020-07-24 Yuki Morita,Futa Kikumatsu,Yujiro Higuchi,Yoshinori Katakura,Kaoru Takegawa
Aspergillus oryzae can secrete large amounts of enzymes. However, the production of abundant secretory proteins triggers the unfolded protein response (UPR) in the endoplasmic reticulum (ER), and it is not clear how ER-associated protein degradation (ERAD) contributes to bulk protein production in A. oryzae. Here we identified AoCdc48, the sole A. oryzae ortholog of Saccharomyces cerevisiae AAA+ ATPase
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Exposure of Agaricus bisporus to Trichoderma aggressivum f. europaeum leads to growth inhibition and induction of an oxidative stress response. Fungal Biol. (IF 2.789) Pub Date : 2020-07-23 Dejana Kosanovic,Helen Grogan,Kevin Kavanagh
Green mould disease of mushroom, Agaricus bisporus,is caused by Trichodermaspecies and can result in substantial crop losses.Label free proteomic analysis of changes in the abundance of A. bisporusproteins following exposure to T. aggressivumsupernatantin vitroindicated increased abundance of proteins associated with an oxidative stress response (zinc ion binding (+6.6 fold); peroxidase activity (5
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Evaluation of genome size and quantitative features of the dolipore septum as taxonomic predictors for the Serendipita 'williamsii' species complex. Fungal Biol. (IF 2.789) Pub Date : 2020-07-17 Jolien Venneman,Julia Sophie Pawlick,Kris Audenaert,Evelyne Meyer,Kristel Demeyere,Leen Leus,Geert Baert,Karl-Heinz Kogel,Geert Haesaert,Danny Vereecke,Katrin Ehlers
Despite multiple taxonomic revisions, several uncertainties at the genus and species level remain to be resolved within the Serendipitaceae family (Sebacinales). This volatile classification is attributed to the limited number of available axenic cultures and the scarcity of useful morphological traits. In the current study, we attempted to discover alternative taxonomic markers not relying on DNA
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