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Involvement of laccase-like enzymes in humic substance degradation by diverse polar soil bacteria Folia Microbiol. (IF 1.73) Pub Date : 2021-01-20 Ha Ju Park, Yung Mi Lee, Hackwon Do, Jun Hyuck Lee, Eungbin Kim, Hyoungseok Lee, Dockyu Kim
Humic substances (HS) in soil are widely distributed in cold environments and account for a significant fraction of soil’s organic carbon. Bacterial strains (n = 281) were isolated at 15 °C using medium containing humic acids (HA), a principal component of HS, from a variety of polar soil samples: 217 from the Antarctic and 64 from the Arctic. We identified 73 potential HA-degrading bacteria based
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Exemplifying endophytes of banana ( Musa paradisiaca ) for their potential role in growth stimulation and management of Fusarium oxysporum f. sp cubense causing panama wilt Folia Microbiol. (IF 1.73) Pub Date : 2021-01-20 Ajit kumar Savani, Ashok Bhattacharyya, Robin Chandra Boro, K. Dinesh, Nanjunda Swamy JC
In the present study, potentiality of endophytic microorganisms such as Rigidiporus vinctus AAU EF, Trichoderma reesei UH EF, and Sphingobacterium tabacisoli UH EB in the management of panama wilt and growth promotion of banana was assessed through artificial inoculation. During the study, a total of 220 bacterial and 110 fungal endophytes were isolated from root, pseudostem, and leaf samples of banana
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A comprehensive review on Morchella importuna : cultivation aspects, phytochemistry, and other significant applications Folia Microbiol. (IF 1.73) Pub Date : 2021-01-19 Krishika Sambyal, Rahul Vikram Singh
Morchella importuna is one of the most highly priced edible mushrooms and a rich source of bioactive substances with numerous beneficial medicinal properties. It has been artificially cultivated in the last few years but due to the unclear mechanism of its fruiting body formation, the stable production has not been achieved yet. This review aims to provide the detailed information about the current
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Simultaneous use of polarization microscopy and dark field techniques in the study of microorganisms Folia Microbiol. (IF 1.73) Pub Date : 2021-01-15 Zdeněk Žižka
Simultaneous application of polarization microscopy and dark field techniques has been used to study the internal structure of microbial cells. The dark field technique displays subtle cell structures like glowing objects on a dark background. In the polarizing microscope, cross polarizing filters along with the first-order quartz compensator and a rotary table show the maximum birefringence of the
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Attenuation of Pseudomonas aeruginosa biofilm by thymoquinone: an individual and combinatorial study with tetrazine-capped silver nanoparticles and tryptophan Folia Microbiol. (IF 1.73) Pub Date : 2021-01-07 Poulomi Chakraborty, Payel Paul, Monika Kumari, Surajit Bhattacharjee, Mukesh Singh, Debasish Maiti, Debabrata Ghosh Dastidar, Yusuf Akhter, Taraknath Kundu, Amlan Das, Prosun Tribedi
Microbial biofilm indicates a cluster of microorganisms having the capability to display drug resistance property, thereby increasing its proficiency in spreading diseases. In the present study, the antibiofilm potential of thymoquinone, a black seed–producing natural molecule, was contemplated against the biofilm formation by Pseudomonas aeruginosa. Substantial antimicrobial activity was exhibited
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Loop-mediated isothermal amplification (LAMP) assay for detection of sesame phyllody phytoplasmas in Vietnam Folia Microbiol. (IF 1.73) Pub Date : 2021-01-06 Nguyen Bao Quoc, Nguyen Thi Thanh Xuan, Nguyen Mai Nghiep, Nguyen Doan Nguyen Phuong, Ton Bao Linh, Nguyen Ngoc Bao Chau, Nguyen Duc Xuan Chuong, Nguyen Chau Nien, Matthew Dickinson
Phloem-limiting phytoplasmas are known to be causal agents of phyllody, which is recognized by the abnormal development of floral structures resulting in serious yield losses in sesame plants. Currently, identification of the various groups of phytoplasmas that cause sesame phyllody (SP) is conducted by nested PCR, RFLP, and multiplex real-time qPCR assays. However, these methods require intensive
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Kinetics of growth and co-production of amylase and protease in novel marine actinomycete, Streptomyces lopnurensis KaM5 Folia Microbiol. (IF 1.73) Pub Date : 2021-01-06 Dalip Singh Rathore, Satya P. Singh
Amylases and proteases are among the industrially most important enzymes for food processing, animal feed, brewing, starch processing, detergents, healthcare, leather processing, and biofuel production. In this study, we investigated the growth kinetics and statistically optimized the co-production of amylase and protease in a phylogenetically novel haloalkaliphilic actinomycete, Streptomyces lopnurensis
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Changes in cell wall structure and protein set in Candida maltosa grown on hexadecane Folia Microbiol. (IF 1.73) Pub Date : 2020-11-27 Anton Zvonarev, Vasilina Farofonova, Ekaterina Kulakovskaya, Tatiana Kulakovskaya, Andrey Machulin, Svyatoslav Sokolov, Vladimir Dmitriev
The yeast Candida maltosa is a model organism for studying adaptive changes in the structure and function of the cell wall when consuming water-insoluble nutrient sources. The cells of C. maltosa that utilize hydrocarbons contain supramolecular structures, so-called “canals” in the cell wall. Differences in protein profiles of culture liquids and cell wall extracts of C. maltosa grown on glucose and
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Simultaneous use of Interphako interference contrast and polarization microscopy in the study of microorganisms Folia Microbiol. (IF 1.73) Pub Date : 2020-11-25 Zdeněk Žižka
Simultaneous application of polarization microscopy and Interphako interference contrast has been used to study the internal structure of algal cells. The interference contrast technique showed fine cell structures (important is the selection of interference colors according to the Mach-Zehnder interferometer setting). In a polarization microscope, the crossed polarization filters together with the
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Main bacterial species causing clinical disease in ornamental freshwater fish in Brazil Folia Microbiol. (IF 1.73) Pub Date : 2020-11-13 Pedro H. M. Cardoso, Luisa Z. Moreno, Carolina H. de Oliveira, Vasco T. M. Gomes, Ana Paula S. Silva, Mikaela R. F. Barbosa, Maria Inês Z. Sato, Simone C. Balian, Andrea M. Moreno
Bacterial diseases are common in ornamental fish, more frequently associated with ubiquitous bacteria from the aquarium environment. The disease can lead to fish mortality and cause high economic losses if not rapidly controlled. The aim of this study was to identify the main causative bacterial agents of infection in ornamental fish with different clinical signs. A total of 126 freshwater fish, from
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Detergent-compatible fungal cellulases Folia Microbiol. (IF 1.73) Pub Date : 2020-11-12 Francois N. Niyonzima
Detergent enzymes are currently added to all powder and liquid detergents that are manufactured. Cellulases, lipases, amylases, and proteases are used in the detergency to replace toxic phosphates and silicates and to reduce high energy consumption. This makes the use of enzymes in detergent formulation cost effective. Fungi are producers of important extracellular enzymes for industrial use. The fungal
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iTRAQ®-based quantitative proteomics reveals the proteomic profiling of methicillin-resistant Staphylococcus aureus -derived extracellular vesicles after exposure to imipenem Folia Microbiol. (IF 1.73) Pub Date : 2020-11-09 Jichun Wang, Junrui Wang, Yanyan Wang, Peng Sun, Xiaohui Zou, Luo Ren, Chunxia Zhang, Enmei Liu
This study sought to reveal the proteomic profiling of methicillin-resistant Staphylococcus aureus (MRSA)-derived extracellular vesicles (EVs) after exposure to imipenem. The advanced isobaric tags for relative and absolute quantitation (iTRAQ®) proteomic approach were used to analyze the alterations in MRSA-derived EV protein patterns upon exposure to imipenem. A total of 1260 EV proteins were identified
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Rapid and sensitive identification of pleural and peritoneal infections by droplet digital PCR Folia Microbiol. (IF 1.73) Pub Date : 2020-11-07 Fangmei Zhou, Shoudong Sun, Xiling Sun, Ye Chen, Xuejing Yang
Pleural and peritoneal infections cause substantial morbidity and mortality. Traditional diagnostic methods rely on the cultivation of clinical samples, which usually takes days to obtain report and holds a low detection sensitivity. In this study, we evaluated a 5-fluorescent-channel droplet digital PCR (ddPCR) system and 5 assay panels for culture-independent rapid pathogen detections directly from
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Bacterial diversity associated with a newly described bioeroding sponge, Cliona thomasi , from the coral reefs on the West Coast of India Folia Microbiol. (IF 1.73) Pub Date : 2020-11-02 Sambhaji Mote, Vishal Gupta, Kalyan De, Mandar Nanajkar, Samir R. Damare, Baban Ingole
The bacterial diversity associated with eroding sponges belonging to the Cliona viridis species complex is scarcely known. Cliona thomasi described from the West Coast of India is a new introduction to the viridis species complex. In this study, we determined the bacterial diversity associated with C. thomasi using next-generation sequencing. The results revealed the dominance of Proteobacteria followed
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Pathogen determination from clinical abscess fluids using metagenomic next-generation sequencing Folia Microbiol. (IF 1.73) Pub Date : 2020-11-02 Yuxi Zhao, Jinlian Chen, Bing Bai, Yu Wang, Jinxin Zheng, Zhijian Yu, Qiwen Deng, Peiyu Li
Abscesses are often clinically manifested as local necrotic tissues in various organs or systems of the human body, which is commonly caused by microbial infection. Rapid and accurate identification of pathogens from clinical abscetic samples would greatly guide a clinician to make the precise choices of the antimicrobial treatment. Here, this study aimed to investigate the application of metagenomic
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Arsenic efflux in Enterobacter cloacae RSN3 isolated from arsenic-rich soil Folia Microbiol. (IF 1.73) Pub Date : 2020-10-31 Biplab Dash, Narayan Sahu, Anup Kumar Singh, S.B Gupta, Ravindra Soni
In the present study, bacterial isolates were screened for arsenic resistance efficiency. Environmental isolates were isolated from arsenic-rich soil samples (i.e., from Rajnandgaon district of Chhattisgarh state, India). Amplification and sequencing of 16S rRNA gene revealed that the isolates were of Bacillus firmus RSN1, Brevibacterium senegalense RSN2, Enterobacter cloacae RSN3, Stenotrophomonas
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Anti-phage serum antibody responses and the outcome of phage therapy Folia Microbiol. (IF 1.73) Pub Date : 2020-10-30 M. Łusiak-Szelachowska, R. Międzybrodzki, W. Fortuna, J. Borysowski, Andrzej Górski
We examined the appearance of serum anti-phage antibodies in 25 patients with chronic sinusitis treated with phage therapy (PT). Approximately 30% of patients with weak antibody responses responded positively to PT, which was similar to the results of treatment achieved in a group of patients with high antibody production. In addition, there was no correlation between antibody level and the outcome
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Study of the effects of mineral salts on the biofilm formation on polypropylene fibers using three quantification methods Folia Microbiol. (IF 1.73) Pub Date : 2020-10-26 Lukáš Bystrianský, Martina Hujslová, Milan Gryndler
The microbial biofilms are ubiquitous in nature and represent important biological entities that affect various aspects of human life. As such, they attracted considerable attention during last decades, with the factors affecting the biofilm development being among the frequently studied topics. In our work, the biofilm was cultivated on the surface of polypropylene fibers in a nutrient medium inoculated
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Integrated use of phosphate-solubilizing Bacillus subtilis strain IA6 and zinc-solubilizing Bacillus sp. strain IA16: a promising approach for improving cotton growth Folia Microbiol. (IF 1.73) Pub Date : 2020-10-24 Iqra Ahmad, Maqshoof Ahmad, Azhar Hussain, Moazzam Jamil
Mineral nutrition of crop plants is one of the major challenges faced by modern agriculture, particularly in arid and semi-arid regions. In alkaline calcareous soils, the availability of phosphorus and zinc is critically less due to their fixation and precipitation as complexes. Farmers use fertilizers to fulfill crop requirements, but their efficacy is less, which increases production costs. Plant
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Biological characteristics and genome analysis of a novel phage vB_KpnP_IME279 infecting Klebsiella pneumoniae Folia Microbiol. (IF 1.73) Pub Date : 2020-10-16 Rongrong Zhang, Feiyang Zhao, Jiuru Wang, Guangqian Pei, Hang Fan, Lilan Zhangxiang, Zhiqiang Mi, Taoxing Shi, Hui Liu, Yigang Tong
Klebsiella pneumoniae (family Enterobacteriaceae) is a gram-negative bacterium that has strong pathogenicity to humans and can cause sepsis, pneumonia, and urinary tract infection. In recent years, the unreasonable use of antibacterial drugs has led to an increase in drug-resistant strains of K. pneumoniae, a serious threat to public health. Bacteriophages, viruses that infect bacteria, are ubiquitous
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Denaturing gradient gel electrophoresis and multi-SIR profiles of soil microbial communities from a karst doline at Aggtelek National Park, Hungary Folia Microbiol. (IF 1.73) Pub Date : 2020-10-08 Márton Mucsi, Gergely Krett, Tibor Szili-Kovács, János Móga, Andrea K. Borsodi
Soils play an important role in the ecosystem of karstic landscapes both as a buffer zone and as a source of acidity to belowground water. Although the microbiota of karstic soils is known to have a great effect on karstification processes, the activity and composition of these communities are largely unknown. This study gives a comparative analysis of soil microbial profiles from different parts of
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Tularemia: a re-emerging tick-borne infectious disease Folia Microbiol. (IF 1.73) Pub Date : 2020-09-28 Derya Karataş Yeni, Fatih Büyük, Asma Ashraf, M. Salah ud Din Shah
Tularemia is a bacterial disease of humans, wild, and domestic animals. Francisella tularensis, which is a Gram-negative coccobacillus-shaped bacterium, is the causative agent of tularemia. Recently, an increase in the number of human tularemia cases has been noticed in several countries around the world. It has been reported mostly from North America, several Scandinavian countries, and certain Asian
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Culturable bacteria from an Alpine coniferous forest site: biodegradation potential of organic polymers and pollutants. Folia Microbiol. (IF 1.73) Pub Date : 2020-09-25 Tanja Berger,Caroline Poyntner,Rosa Margesin
The potential of the culturable bacterial community from an Alpine coniferous forest site for the degradation of organic polymers and pollutants at low (5 °C) and moderate (20 °C) temperatures was evaluated. The majority of the 68 strains belonged to the phylum Proteobacteria (77%). Other strains were related to Bacteroidetes (12%), Alphaproteobacteria (4%), Actinobacteria (3%), and Firmicutes (3%)
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Edible marine algae: a new source for anti-mycobacterial agents. Folia Microbiol. (IF 1.73) Pub Date : 2020-09-25 Goutam Mukherjee,Balaram Mukhopadhyay,Alok Kumar Sil
Tuberculosis is a dreaded disease, which causes innumerable death worldwide. The emergence of drug resistance strains makes the situation devastating. Therefore, for better management of public health, it is mandatory to search for new anti-mycobacterial agents. In this context, the current study investigated two edible marine algae, Ulva lactuca and Ulva intestinalis, for the probable source of new
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Bacterial cross talk with gut microbiome and its implications: a short review. Folia Microbiol. (IF 1.73) Pub Date : 2020-09-19 Rajesh P Shastry,P D Rekha
Human gut microbiota exists in a complicated symbiotic relationship which postulates to impact health and disease conditions on the host. Interestingly, the gut microbiome shows different mechanisms to regulate host physiology and metabolism including cell-to-cell communications. But microbiota imbalance is characterized to change in the host normal functioning and lead to the development and progression
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Enzymatic degradation of sulphonated azo dye using purified azoreductase from facultative Klebsiella pneumoniae. Folia Microbiol. (IF 1.73) Pub Date : 2020-09-18 Shweta Dixit,Sanjeev Garg
Heterologously expressed and purified azoreductase enzyme from facultative Klebsiella pneumoniae was used to degrade sulphonated azo dye. Methyl orange (MO) was used as the model dye to study the azo dye decolorization potential of the purified enzyme at different conditions. The enzyme had maximum activity at 40 °C and pH 8.0. The enzyme was observed to be thermo-stable as some enzyme activity was
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Capability of Penicillium oxalicum y2 to release phosphate from different insoluble phosphorus sources and soil. Folia Microbiol. (IF 1.73) Pub Date : 2020-09-17 Junpeng Wang,Yang-Guo Zhao,Farhana Maqbool
Due to insufficient amount of soluble phosphate and poor persistence of traditional chemical phosphate fertilizers in agricultural soils, the eco-friendly and sustainable phosphorus sources for crops are urgently required. The efficient phosphate-releasing fungal strain designated y2 was isolated and identified by the internal transcribed spacer of rDNA as Penicillium oxalicum y2. When lecithin, Ca3(PO4)2
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Characteristics of microbial community of soil subjected to industrial production of antibiotics. Folia Microbiol. (IF 1.73) Pub Date : 2020-09-08 Martina Borčinová,Anastasiya Pitkina,Helena Marešová,Václav Štěpánek,Andrea Palyzová,Pavel Kyslík
Ecosystems worldwide are exposed to pollutants connected to the industrial production of pharmaceuticals. The objective of this study was to study the composition and characteristics of the soil microbial communities that had been exposed to long-term selection pressure caused by the industrial production of penicillin G. Soil samples from four sites among the penicillin G production plant were analysed
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Antibacterial activity and mechanism of three isomeric terpineols of Cinnamomum longepaniculatum leaf oil. Folia Microbiol. (IF 1.73) Pub Date : 2020-09-07 Jinfeng Huang,Liyan Yang,Yue Zou,Sican Luo,Xin Wang,Yujuan Liang,Yonghua Du,Ruizhang Feng,Qin Wei
α-Terpineol, terpinen-4-ol, and δ-terpineol, isomers of terpineol, are among the compounds that give Cinnamomum longepaniculatum leaf oil its distinguished pleasant smell. The objective of this study was to evaluate the antimicrobial activity of these three isomeric terpineols. The determination of antibacterial activity was based on the minimum inhibition concentration (MIC) and minimum bactericide
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Shared bicycle microbial community: a potential antibiotic-resistant bacteria warehouse. Folia Microbiol. (IF 1.73) Pub Date : 2020-09-05 Yuqi Wu,Jiahui Xie,Jing Li,Junyi Zhao,Shihao Qiao,Yuqing Li,Jumei Zeng
Shared bicycle is an emerging form of public transportation in China and around the world. However, the bacterial community and drug-resistant microbiome on these bicycles have not been reported. Samples from 10 shared bicycles were observed by scanning electron microscopy (SEM). Nine samples collected from 90 shared bicycles in three different kinds of location (hospital, metro station, shopping mall)
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Solid-state fermentation with Pleurotus ostreatus improves the nutritive value of corn stover-kudzu biomass. Folia Microbiol. (IF 1.73) Pub Date : 2020-08-30 Uchenna Y Anele,Felicia N Anike,Alexia Davis-Mitchell,Omoanghe S Isikhuemhen
A batch culture technique was used to evaluate dry matter (DM) digestibility of corn stover (Zea mays L.) and kudzu, Pueraria montana (Lour.) Merr. after solid-state fermentation (SSF) with a white-rot fungus, Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm. Five dietary treatments consisting of mixtures of corn stover (C) and kudzu (K) in varying ratios, (1) 100C:0K, (2) 75C:25K, (3) 50C:50K, (4) 25C:75K
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RamA, a transcriptional regulator conferring florfenicol resistance in Leclercia adecarboxylata R25. Folia Microbiol. (IF 1.73) Pub Date : 2020-08-28 Cong Cheng,Yuanyuan Ying,Danying Zhou,Licheng Zhu,Junwan Lu,Aifang Li,Qiyu Bao,Mei Zhu
Due to the inappropriate use of florfenicol in agricultural practice, florfenicol resistance has become increasingly serious. In this work, we studied the novel florfenicol resistance mechanism of an animal-derived Leclercia adecarboxylata strain R25 with high-level florfenicol resistance. A random genomic DNA library was constructed to screen the novel florfenicol resistance gene. Gene cloning, gene
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Impact of cultivation strategy, freeze-drying process, and storage conditions on survival, membrane integrity, and inactivation kinetics of Bifidobacterium longum. Folia Microbiol. (IF 1.73) Pub Date : 2020-08-27 Regina Haindl,Alexandra Neumayr,Anika Frey,Ulrich Kulozik
Bifidobacterium longum, one of the main microorganisms in the human gut, is used as an adjunct to lactic acid starter cultures or sold as a probiotic product. Therefore, Bifidobacterium longum cell suspensions get freeze-dried with protective additives to prevent activity losses. To date, investigations covering growth and inactivation kinetics of Bifidobacterium longum during the whole process (cultivation
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Benzylidene-carbonyl compounds are active against itraconazole-susceptible and itraconazole-resistant Sporothrix brasiliensis. Folia Microbiol. (IF 1.73) Pub Date : 2020-08-21 Stefanie Bressan Waller,Marlete Brum Cleff,Márcia Kutscher Ripoll,Mário Carlos Araújo Meireles,Marina Themoteo Varela,João Paulo Dos S Fernandes
We evaluated the antifungal activity of benzylidene-carbonyl compounds (LINS03) based on the structure of gibbilimbol from Piper malacophyllum Linn. Five analogues (1–5) were synthetized following a classic aldol condensation between an aromatic aldehyde and a ketone, under basic conditions. These were tested against itraconazole-susceptible (n = 3) and itraconazole-resistant (n = 5) isolates of Sporothrix
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Clinical and histopathological changes in rainbow trout Oncorhynchus mykiss experimentally infected with fungus Bradymyces oncorhynchi. Folia Microbiol. (IF 1.73) Pub Date : 2020-07-29 J Řehulka,M Kolařík,V Hubka
Bradymyces oncorhynchi is a poorly known melanised fungal species that has been isolated only from a hyperaemic focus near the enlarged spleen of a rainbow trout. Although the pathogenicity of this species to fish is suspected, it has not been fully confirmed. Four laboratory experiments were conducted to test the effect of the fungus on the health of rainbow trout fingerlings. Mycelia were cultivated
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Correction to: Recent progress on engineering microbial alginate lyases towards their versatile role in biotechnological applications. Folia Microbiol. (IF 1.73) Pub Date : 2020-07-22 Shivakumar Renuka Dharani,Ramachandran Srinivasan,Regunathan Sarath,Mohandass Ramya
The original version of the article unfortunately contained an error
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Growth, fatty, and amino acid profiles of the soil alga Vischeria sp. E71.10 (Eustigmatophyceae) under different cultivation conditions. Folia Microbiol. (IF 1.73) Pub Date : 2020-07-21 Daniel Remias,Cecilia Nicoletti,Klaus Krennhuber,Bettina Möderndorfer,Linda Nedbalová,Lenka Procházková
In this study, a unicellular soil alga isolated from farmland in Germany was surveyed. The investigation of the hypervariable molecular markers ITS1 rDNA and ITS2 rDNA identified strain E71.10 as conspecific with Vischeria sp. SAG 51.91 (Eustigmatophyceae). The culture was tested for biomass generation and for the yield of fatty acids and amino acids. The survey included four different culture conditions
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16S rRNA molecular profiling of heavy metal tolerant bacterial communities isolated from soil contaminated by electronic waste. Folia Microbiol. (IF 1.73) Pub Date : 2020-07-21 Pankaj Kumar,M H Fulekar,R Y Hiranmai,Ramesh Kumar,Rajesh Kumar
Electronic waste is an evolving source of harmful pollutants in our surrounding environments and considered to be perilous as it contains toxic metals such as chromium, cadmium, lead, mercury, zinc, and nickel in huge quantities. Heavy metals are harmful contaminants and accumulated in the environment due to various anthropogenic activities. The present study was conducted to isolate and characterize
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Genetic analysis of Streptomyces albus J1074 mia mutants suggests complex relationships between post-transcriptional tRNAXXA modifications and physiological traits. Folia Microbiol. (IF 1.73) Pub Date : 2020-07-17 Oksana Koshla,Volodymyr Kravets,Yuriy Dacyuk,Iryna Ostash,Roderich Süssmuth,Bohdan Ostash
Proteins MiaA and MiaB catalyze sequential isopentenylation and methylthiolation, respectively, of adenosine residue in 37th position of tRNAXXA. The mia mutations were recently shown by us to affect secondary metabolism and morphology of Streptomyces. However, it remained unknown as to whether both or one of the aforementioned modifications is critical for colony development and antibiotic production
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Establishment and evaluation of a method for efficient screening of Clostridium butyricum. Folia Microbiol. (IF 1.73) Pub Date : 2020-07-03 Zhi Yi,Faizan A Sadiq,Hongchao Wang,Jianxin Zhao,Hao Zhang,Wenwei Lu,Wei Chen
At present, the traditional methods for the screening of Clostridium butyricum are not sufficiently selective and efficient. Therefore, it is necessary to establish a targeted and efficient screening method for the detection of C. butyricum. Bioinformatics was used in this study to find C. butyricum specific genes, and species-specific primers were designed based on the conserved regions of the targeted
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Evaluation of antifungal activity of cinnamaldehyde against Cryptococcus neoformans var. grubii. Folia Microbiol. (IF 1.73) Pub Date : 2020-07-02 Neelabh,Karuna Singh
Cryptococcosis is a potentially fatal fungal disease which has aggrandized with the emergence of AIDS and antifungal resistance. The currently used antifungals lack the broad-spectrum activity and result in several toxicities during long treatment regimens. Thus, the present study aims to evaluate the antifungal activity of cinnamaldehyde against Cryptococcus neoformans var. grubii, the etiological
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Insufficient repeatability and reproducibility of MALDI-TOF MS-based identification of MRSA. Folia Microbiol. (IF 1.73) Pub Date : 2020-07-02 Veronika Paskova,Katerina Chudejova,Anna Sramkova,Lucie Kraftova,Vladislav Jakubu,Efthimia A Petinaki,Helena Zemlickova,Katerina Neradova,Costas C Papagiannitsis,Jaroslav Hrabak
Rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) is essential for proper initial antibiotic therapy and timely set up of hygienic measures. Recently, detection of MRSA using MALDI-TOF mass spectrometer mediated by the peptide—phenol-soluble modulin (PSM-mec)—linked to the class A mec gene complex present in SCCmec cassettes types II, III, and VIII of MRSA strains, has been
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Plasmid gene for putative integral membrane protein affects formation of lipopolysaccharide and motility in Azospirillum brasilense Sp245. Folia Microbiol. (IF 1.73) Pub Date : 2020-06-30 Lilia P Petrova,Stella S Yevstigneyeva,Yulia A Filip'echeva,Andrei V Shelud'ko,Gennady L Burygin,Elena I Katsy
The bacterium Azospirillum brasilense can swim and swarm owing to the work of polar and lateral flagella. Its major surface glycopolymers consist of lipopolysaccharides (LPS) and Calcofluor-binding polysaccharides (Cal+ phenotype). Motility and surface glycopolymers are important for the interactions of plant-associated bacteria with plants. The facultative plant endophyte A. brasilense Sp245 produces
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Candida albicans culture from a rectal swab can be associated with newly diagnosed colorectal cancer. Folia Microbiol. (IF 1.73) Pub Date : 2020-06-29 Lubomír Starý,Kristýna Mezerová,Kamil Vysloužil,Pavel Zbořil,Pavel Skalický,Martin Stašek,Vladislav Raclavský
Plenty of metagenomic studies have suggested possible associations between microbiome composition and colorectal cancer (CRC). However, these techniques are not economic enough for routine use so far. Therefore, we explored the possibility to detect species associated with colorectal cancer by conventional culture from rectal swab. Fifty-two patients newly diagnosed for adenoma/CRC and 52 age-matched
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Application of yeast surface display system in expression of recombinant pediocin PA-1 in Saccharomyces cerevisiae. Folia Microbiol. (IF 1.73) Pub Date : 2020-06-24 Thu Pham Anh Nguyen,Thu Thi Minh Nguyen,Nghia Hieu Nguyen,Tri Nhan Nguyen,Thao Thi Phuong Dang
Pediocin PA-1 is a bacteriocin that shows strongly anti-microbial activity against some Gram-positive pathogens such as Listeria monocytogenes, Staphylococcus aureus, and Enterococcus faecalis. With the broad inhibitory spectrum as well as high-temperature stability, pediocin has a potential application in the food preservation and pharmaceutical industry. Pediocin has been studied to express in many
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Recent progress on engineering microbial alginate lyases towards their versatile role in biotechnological applications. Folia Microbiol. (IF 1.73) Pub Date : 2020-06-04 Shivakumar Renuka Dharani,Ramachandran Srinivasan,Reghunathan Sarath,Mohandass Ramya
Biomass feedstock is an efficient and harmless source of energy. their are various sources of feedstock, such as plant, microbial, macro, and microalgae, and agricultural waste. The major component in biomass feedstock material is a polysaccharide, such as cellulose, cellobiose, starch, and alginate. Alginate is mainly found in macroalgae as one of the significant polysaccharide components. It is made
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Salt tolerance of nitrate reductase in Halomonas sp. B01. Folia Microbiol. (IF 1.73) Pub Date : 2020-06-01 Te Wang,Yujing Li,Linghua Zhang,Weifeng Liu,Yimin Zhu
A systematic study on the lack of dissimilatory nitrate reductase (NAR) properties in Halomonas strains had been reported so far. The effects of different factors on Halomonas sp. B01 NAR activity were investigated. The salt tolerance of NAR was characterized. The denitrification process under high salt conditions was reported. Halomonas sp. B01 expressed membrane-bound NAR under induced culture by
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Survival of Brucella abortus S19 and other Brucella spp. in the presence of oxidative stress and within macrophages. Folia Microbiol. (IF 1.73) Pub Date : 2020-05-28 Jens Jacob,Antje Finke,Martin Mielke
The evolutionary “success” of the genus Brucella depends on the ability to persist both in the environment as well as inside of even activated macrophages of the animal host. For that, the Brucellae produce catalase and superoxide dismutase to defend against oxidative stress. Since the deletion of the mglA gene in the B. abortus S19 vaccine strain resulted not only in an increased tolerance to H2O2
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Chlorinated plastoquinone analogs that inhibit Staphylococcus epidermidis and Candida albicans growth. Folia Microbiol. (IF 1.73) Pub Date : 2020-05-26 Emel Mataracı Kara,Nilüfer Bayrak,Hatice Yıldırım,Mahmut Yıldız,Berna Ozbek Celik,Amaç Fatih Tuyun
Infectious diseases are the significant global health problem because of drug resistance to most classes of antimicrobials. Interest is growing in the development of new antimicrobials in pharmaceutical discovery. For that reason, the urgency for scientists to find and/or develop new important molecules is needed. Many natural active molecules that exhibit various biological activities have been isolated
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Combination of non-thermal plasma and subsequent antibiotic treatment for biofilm re-development prevention. Folia Microbiol. (IF 1.73) Pub Date : 2020-05-19 Jaroslav Julák,Eva Vaňková,Markéta Válková,Petra Kašparová,Jan Masák,Vladimír Scholtz
The influence of non-thermal plasma (NTP) treatment on the prevention of antibiotic resistance of microbial biofilms was studied. Staphylococcus epidermidis and Escherichia coli bacteria and a yeast Candida albicans, grown on the surface of Ti-6Al-4V alloy used in the manufacture of prosthetic implants, were employed. Their biofilms were exposed to NTP produced by DC cometary discharge and subsequently
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Keratinolytic enzyme-mediated biodegradation of recalcitrant poultry feathers waste by newly isolated Bacillus sp. NKSP-7 under submerged fermentation. Folia Microbiol. (IF 1.73) Pub Date : 2020-05-16 Ikram Ul Haq,Fatima Akram,Zuriat Jabbar
Microbial and enzymatic degradation of keratin waste is more preferred over various conventional approaches which are costly and not environmentally suitable. Diverse niches are auspicious for the discovery of new microorganisms. To discover novel keratinolytic bacteria, 60 isolates from different poultry dumping sites were initially screened, and among these found a potent keratinolytic isolate (NKSP-7)
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Biphasic optimization approach for maximization of lipid production by the microalga Chlorella pyrenoidosa. Folia Microbiol. (IF 1.73) Pub Date : 2020-05-15 Kateřina Sukačová,Diana Búzová,Jan Červený
The aim of the study was to identify the optimum cultivation conditions for the microalgal growth and lipid production of the oleaginous microalga Chlorella pyrenoidosa Chick (IPPAS C2). Moreover, an appropriate NO3- concentration in the cultivation medium for maximized lipid accumulation was determined. The experimental design involved a biphasic cultivation strategy with an initial biomass accumulating
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Exoenzyme-producing halophilic bacteria from the former Lake Texcoco: identification and production of n-butyl oleate and bioactive peptides. Folia Microbiol. (IF 1.73) Pub Date : 2020-05-15 Raúl Balam Martínez-Pérez,Jorge Alberto Rodríguez,Luis A Cira-Chávez,Luc Dendooven,Gustavo Viniegra-González,Isabel Estrada-Alvarado
Halophilic bacterias from saline soil from former Lake Texcoco were isolated, identified based on 16 rRNA and tested to produce glucolytic, nucleolytic, proteolytic and lipolytic exoenzymes. The Bacillus, Virgibacillus, Kocuria, Salinicoccus, Gracilibacillus, Halobacillus, Tenuibacillus and Nesterekonia genera where identified. Lipase/eserases and proteases from Nesterenkonia sp. and Nesterenkonia
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The cross-sectional study of Toxoplasma gondii seroprevalence in selected groups of population in Slovakia. Folia Microbiol. (IF 1.73) Pub Date : 2020-05-15 Miroslava Fecková,Daniela Antolová,Martin Janičko,Halánová Monika,Gabriela Štrkolcová,Mária Goldová,Tatiana Weissová,Branislav Lukáč,Mária Nováková
Toxoplasma gondii is an obligate intracellular protozoan parasite with domestic or free-living members of family Felidae known as the only definitive hosts of the parasite. The study aimed to evaluate the seroprevalence of toxoplasmosis in selected population groups in Slovakia and to analyse the infection risk factors. Totally 1536 serum samples of voluntary respondents were examined by EIA Toxoplasma
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A statistical approach for enhanced production of β-galactosidase from Paracoccus sp. and synthesis of galacto-oligosaccharides. Folia Microbiol. (IF 1.73) Pub Date : 2020-05-11 Pooja Kalathinathan,Gothandam Kodiveri Muthukaliannan
A new β-galactosidase-producing bacterium KGP, isolated from the Bay of Bengal, was identified as Paracoccus marcusii through morphology, biochemistry and 16S rRNA sequencing. This study is the first report on the production of β-galactosidase from P. marcusii. The medium components for the high yield of β-galactosidase were optimised using response surface methodology (RSM). A set of 17 experiments
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A sporadic case of acute Q fever and identification of the animal source of the infection. Folia Microbiol. (IF 1.73) Pub Date : 2020-05-08 Hacène Medkour,Bernard Davoust,Manolis Angelakis,Richard Thiéry,Didier Raoult,Elodie Rousset,Philippe Parola,Carole Eldin
Q fever is a zoonosis. Humans are infected through the inhalation of Coxiella burnetii particles that are dispersed into the air from the birth products or faeces of ruminants. Major outbreaks can occur in association with farming activities. C. burnetii can be disseminated by wind up to several tens of kilometres and infect humans far from its zoonotic source. As a result, the sources of sporadic
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Remedial potential of bacterial and fungal strains (Bacillus subtilis, Aspergillus niger, Aspergillus flavus and Penicillium chrysogenum) against organochlorine insecticide Endosulfan. Folia Microbiol. (IF 1.73) Pub Date : 2020-05-07 Khuram Shahzad Ahmad
Endosulfan, an organochlorine insecticide, is known to cause detrimental effects to the environment and human health due to its excessive usage. Its highly toxic nature calls for an environmental-friendly approach for its detoxification. Environmental transformation of Endosulfan was assessed through biodegradation by isolated and cultured soil microbes (Bacillus subtilis (BS), Aspergillus niger (AN)
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Corn stover induces extracellular laccase activity in Didymosphaeria sp. (syn. = Paraconiothyrium sp.) and exhibits increased in vitro ruminal digestibility when treated with this fungal species. Folia Microbiol. (IF 1.73) Pub Date : 2020-05-06 Marina Arredondo-Santoyo,José Herrera-Camacho,Ma Soledad Vázquez-Garcidueñas,Gerardo Vázquez-Marrufo
Fungi can improve stover digestibility due to their ability to secrete oxidative enzymes that depolymerize lignin, allowing the rumen microorganisms to access the polysaccharides of the plant cell wall. Some ascomycetes have shown good delignification capability; however, they have been scarcely evaluated for their ability to improve corn stover (CS) ruminal digestibility. We evaluated the laccase
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Detection of 16SrVI and 16SrIX phytoplasma groups in pot marigold and tickseed plants in northeastern Iran. Folia Microbiol. (IF 1.73) Pub Date : 2020-05-05 Sara Gharouni-Kardani,Mahnaz Ashnayi,Assunta Bertaccini
Pot marigold and tickseed are ornamental plants with many medicinal and cosmetic uses and for landscape, respectively. During a survey in 2018, phyllody symptoms were observed in high percentages in these plants in some regions of the Razavi Khorasan province (northeastern Iran). Total DNA was extracted from symptomatic and asymptomatic plants and polymerase chain reaction was carried on using universal