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Enhancing biomass conservation and enzymatic hydrolysis of sweet sorghum bagasse by combining pretreatment with ensiling and NaOH Front. Microbiol. (IF 5.2) Pub Date : 2024-03-15 Shuai Zhao, Hanyan Li, Tawatchai Sumpradit, Aman Khan, El-Sayed Salama
Lignocellulosic pretreatment is an important stage in biomass utilization, which usually requires high input. In this study, a low-cost method using combined ensiling and NaOH was developed for lignocellulosic pretreatment. Sweet sorghum bagasse (SSB) was ensiled for 21 days and then treated with diluted NaOH (0%, 1%, and 2%) for fermentation. The results showed that the application of Lactobacillus
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Animal models of Klebsiella pneumoniae mucosal infections Front. Microbiol. (IF 5.2) Pub Date : 2024-03-15 Lucas Assoni, Ana Julia Melo Couto, Brenda Vieira, Bárbara Milani, Alice Souza Lima, Thiago Rojas Converso, Michelle Darrieux
Klebsiella pneumoniae is among the most relevant pathogens worldwide, causing high morbidity and mortality, which is worsened by the increasing rates of antibiotic resistance. It is a constituent of the host microbiota of different mucosa, that can invade and cause infections in many different sites. The development of new treatments and prophylaxis against this pathogen rely on animal models to identify
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Multi-omics analyses reveal interactions between the skin microbiota and skin metabolites in atopic dermatitis Front. Microbiol. (IF 5.2) Pub Date : 2024-03-15 Kaikai Huang, Fang Li, Yingyao Liu, Baoying Liang, Pinghua Qu, Linlin Yang, Shanshan Han, Wenjun Li, Xiumei Mo, Lei Dong, Ying Lin
IntroductionAtopic dermatitis (AD) is one of the most common inflammatory skin diseases. Skin microecological imbalance is an important factor in the pathogenesis of AD, but the underlying mechanism of its interaction with humans remains unclear.Methods16S rRNA gene sequencing was conducted to reveal the skin microbiota dynamics. Changes in skin metabolites were tracked by LC–MS metabolomics. We then
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Comparative analysis of biofilm characterization of probiotic Escherichia coli Front. Microbiol. (IF 5.2) Pub Date : 2024-03-15 Huiping Liu, Jingfang Ma, Pan Yang, Feng Geng, Xueling Li, Junhong Lü, Yadi Wang
Biofilms are thought to play a vital role in the beneficial effects of probiotic bacteria. However, the structure and function of probiotic biofilms are poorly understood. In this work, biofilms of Escherichia coli (E. coli) Nissle 1917 were investigated and compared with those of pathogenic and opportunistic strains (E. coli MG1655, O157:H7) using crystal violet assay, confocal laser scanning microscopy
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Soil microbial community are more sensitive to ecological regions than cropping systems in alpine annual grassland of the Qinghai-Tibet Plateau Front. Microbiol. (IF 5.2) Pub Date : 2024-03-15 Feng Luo, Wenbo Mi, Wenhui Liu, Xiang Ma, KaiQiang Liu, Zeliang Ju, Wen Li
IntroductionModern agriculture emphasizes the design of cropping systems using ecological function and production services to achieve sustainability. The functional characteristics of plants (grasses vs. legumes) affect changes in soil microbial communities that drive agroecosystem services. Information on the relationship between legume-grass mixtures and soil microorganisms in different ecological
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Changes in soil oxidase activity induced by microbial life history strategies mediate the soil heterotrophic respiration response to drought and nitrogen enrichment Front. Microbiol. (IF 5.2) Pub Date : 2024-03-15 Weirong Zhuang, Yong Li, Xiaoming Kang, Liang Yan, Xiaodong Zhang, Zhongqing Yan, Kerou Zhang, Ao Yang, Yuechuan Niu, Xiaoshun Yu, Huan Wang, Miaomiao An, Rongxiao Che
Drought and nitrogen deposition are two major climate challenges, which can change the soil microbial community composition and ecological strategy and affect soil heterotrophic respiration (Rh). However, the combined effects of microbial community composition, microbial life strategies, and extracellular enzymes on the dynamics of Rh under drought and nitrogen deposition conditions remain unclear
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Morphological and phylogenetic analyses reveal two new Penicillium species isolated from the ancient Great Wall loess in Beijing, China Front. Microbiol. (IF 5.2) Pub Date : 2024-03-15 Ruina Liang, Qiqi Yang, Ying Li, Guohua Yin, Guozhu Zhao
IntroductionPenicillium species exhibit a broad distribution in nature and play a crucial role in human and ecological environments.MethodsTwo Penicillium species isolated from the ancient Great Wall loess in the Mentougou District of Beijing, China, were identified and described as new species, namely, Penicillium acidogenicum and P. floccosum, based on morphological characteristics and phylogenetic
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The accumulation of active ingredients of Polygonatum cyrtonema Hua is associated with soil characteristics and bacterial community Front. Microbiol. (IF 5.2) Pub Date : 2024-03-15 Qingyou Zhang, Yunzhang Cai, Luping Zhang, Mei Lu, Luyun Yang, Dekai Wang, Qiaojun Jia
IntroductionWith the increasing demand for health products derived from Polygonati rhizoma (PR), people begin to artificially plant Polygonatum cyrtonema Hua (P. cyrtonema) in the middle and lower reaches of the Yangtze River. To promote P. cyrtonema cultivation and increase farmers’ income, efforts are needed to understand the ways to obtain high-quality PR under artificial cultivation conditions
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Effects of Liqi Tongbian decoction on gut microbiota, SCFAs production, and 5-HT pathway in STC rats with Qi Stagnation Pattern Front. Microbiol. (IF 5.2) Pub Date : 2024-03-15 Qihong Liu, Danfeng Ke, Youqin Chen, Aling Shen, Liya Liu, Lunan Hu, Yan Ren, Wenyi Fang, Peilin Zhao, Thomas J. Sferraf, Yunfeng Luo, Xiao Ke
Slow transit constipation (STC) is a common and debilitating condition characterized by delayed colonic transit and difficulty in fecal expulsion, significantly impacting patients’ physical and mental wellbeing as well as their overall quality of life. This study investigates the therapeutic potential of Liqi Tongbian Decoction (LTD) in the treatment of STC, especially in cases involving the context
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In vitro and in vivo evaluation of the biofilm-degrading Pseudomonas phage Motto, as a candidate for phage therapy Front. Microbiol. (IF 5.2) Pub Date : 2024-03-15 Prasanth Manohar, Belinda Loh, Dann Turner, Ramasamy Tamizhselvi, Marimuthu Mathankumar, Namasivayam Elangovan, Ramesh Nachimuthu, Sebastian Leptihn
Infections caused by Pseudomonas aeruginosa are becoming increasingly difficult to treat due to the emergence of strains that have acquired multidrug resistance. Therefore, phage therapy has gained attention as an alternative to the treatment of pseudomonal infections. Phages are not only bactericidal but occasionally show activity against biofilm as well. In this study, we describe the Pseudomonas
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A meta-analysis on the role of sonication in the diagnosis of cardiac implantable electronic device-related infections Front. Microbiol. (IF 5.2) Pub Date : 2024-03-15 Daniela Araújo, João P. Martins, Stephanie Lopes Ferreira, Sandra Mota, Pedro L. Ferreira, Rui Pimenta
IntroductionOne of the biggest obstacles in diagnosing Implant-Associated Infections is the lack of infection criteria and standardized diagnostic methods. These infections present a wide range of symptoms, and their diagnosis can be hampered by the formation of microbial biofilms on the surface of implants. This study aimed to provide insight into the performance of sonication in the diagnosis of
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Matrine reverses the resistance of Haemophilus parasuis to cefaclor by inhibiting the mutations in penicillin-binding protein genes (ftsI and mrcA) Front. Microbiol. (IF 5.2) Pub Date : 2024-03-15 JingChao Zhao, Wen Yang, Hui Deng, Dong Li, QianYong Wang, LingXian Yi, QiHong Kuang, Rui Xu, Di Li, RuoNan Li, DaoJin Yu, Bo Yang
IntroductionMatrine (MT) is a potential resistance reversal agent. However, it remains unclear whether MT can reverse the resistance of Haemophilus parasuis (H. parasuis) to β-lactams, and, if so, by what mechanism MT works.MethodsWe screened one cefaclor (CEC)-resistant strain (clinical strain C7) from eight clinical (H. parasuis) strains and determined the underlying resistance mechanism. Then, we
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The host sex contributes to the endophytic bacterial community in Sargassum thunbergii and their receptacles Front. Microbiol. (IF 5.2) Pub Date : 2024-03-15 Yayun Zhao, Tao Sun, Yang Li, Zhibo Yang, Jun Chen, Jing Wang, Xinlong Yu, Xuexi Tang, Hui Xiao
Endophytic bacteria have a complex coevolutionary relationship with their host macroalgae. Dioecious macroalgae are important producers in marine ecosystems, but there is still a lack of research on how sex influences their endophytic bacteria. In this study, the endophytic bacterial communities in male and female S. thunbergii and their reproductive tissues (receptacles) were compared using culture
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Bee chitosan nanoparticles loaded with apitoxin as a novel approach to eradication of common human bacterial, fungal pathogens and treating cancer Front. Microbiol. (IF 5.2) Pub Date : 2024-03-15 Mohamed Sharaf, Abdullah A. Zahra, Maha Alharbi, Alsayed E. Mekky, Abdelrazeq M. Shehata, Abdulsalam Alkhudhayri, Ahmed M. Ali, Ebtesam A. Al Suhaimi, Shadi A. Zakai, Norah Al Harthi, Chen-Guang Liu
Antimicrobial resistance is one of the largest medical challenges because of the rising frequency of opportunistic human microbial infections across the globe. This study aimed to extract chitosan from the exoskeletons of dead bees and load it with bee venom (commercially available as Apitoxin [Api]). Then, the ionotropic gelation method would be used to form nanoparticles that could be a novel drug-delivery
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Colonization of root endophytic fungus Serendipita indica improves drought tolerance of Pinus taeda seedlings by regulating metabolome and proteome Front. Microbiol. (IF 5.2) Pub Date : 2024-03-15 Chu Wu, Yujie Yang, Yun Wang, Wenying Zhang, Honggang Sun
Pinus taeda is an important forest tree species for plantations because of its rapid growth and high yield of oleoresins. Although P. taeda plantations distribute in warm and wet southern China, drought, sometime serious and long time, often occurs in the region. To explore drought tolerance of P. taeda and usage of beneficial microorganisms, P. taeda seedlings were planted in pots and were inoculated
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Effect of bioaugmentation on gas production and microbial community during anaerobic digestion in a low-temperature fixed-bed reactor Front. Microbiol. (IF 5.2) Pub Date : 2024-03-14 Yunlong Wang, Xiaoya An, Jian Wang, Xinbo Jiang, Xue Li, Jiamin Yin, Weidong Wang, Jin Piao, Hongyan Zhao, Zongjun Cui
Low temperature is one of the limiting factors for anaerobic digestion in cold regions. To improve the efficiency of anaerobic digestion for methane production in stationary reactors under low-temperature conditions, and to improve the structure of the microbial community for anaerobic digestion at low temperatures. We investigated the effects of different concentrations of exogenous Methanomicrobium
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Differential stability of bacterial photosynthetic apparatus of Rhodobacter alkalitolerans strain JA916T under alkaline and light environment Front. Microbiol. (IF 5.2) Pub Date : 2024-03-14 Mohammad Yusuf Zamal, Saikiran Madireddi, Nageswara Rao Mekala, Venkata Ramana Chintalapati, Rajagopal Subramanyam
In purple bacteria, photosynthesis is performed by densely packed pigment-protein complexes, including the light-harvesting complexes known as RC-LH1 and LH2, with carotenoids to assist in the functioning of photosynthesis. Most photosynthetic bacteria are exposed to various abiotic stresses such as light, temperature, alkalinity–acidity, and salinity. Rhodobacter (R.) alkalitolerans was discovered
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Soil bacterial community in a photovoltaic system adopted different survival strategies to cope with small-scale light stress under different vegetation restoration modes Front. Microbiol. (IF 5.2) Pub Date : 2024-03-14 Zhongxin Luo, Jiufu Luo, Sainan Wu, Xiaolin Luo, Xin Sui
Solar photovoltaic (PV) power generation is a major carbon reduction technology that is rapidly developing worldwide. However, the impact of PV plant construction on subsurface microecosystems is currently understudied. We conducted a systematic investigation into the effects of small-scale light stress caused by shading of PV panels and sampling depth on the composition, diversity, survival strategy
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Mice, myeloid cells, and dengue: a new model for unraveling vascular leakage mysteries Front. Microbiol. (IF 5.2) Pub Date : 2024-03-14 Takeshi Kurosu, Yusuke Sakai, Yasusi Ami, Masayuki Shimojima, Tomoki Yoshikawa, Shuetsu Fukushi, Noriyo Nagata, Tadaki Suzuki, Hideki Ebihara, Masayuki Saijo
IntroductionSevere dengue is thought to be caused by an excessive host immune response.MethodsTo study the pathogenesis of severe dengue, we developed a novel model using LysM Cre+Ifnarflox/flox mice carrying depleted Ifnar expression only in subsets of murine myeloid cells.ResultsAlthough dengue virus (DENV) clinical isolates were not virulent in LysM Cre+Ifnarflox/flox mice, mouse-adapted DV1-5P7Sp
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Salinity tolerance and desalination properties of a Haematococcus lacustris strain from eastern Hungary Front. Microbiol. (IF 5.2) Pub Date : 2024-03-14 István Bácsi, Aida Figler, Edina Simon, Majd Muwafaq Yaqoob, Kamilla Márton, Viktória B-Béres
Nowadays the increasing amount of saline wastewaters has given rise to various biological desalination processes, among which the application possibilities of microalgae represents a priority research area. Next to “real” aquatic species (members of phytoplankton or phytobenthon), species from ephemeral aquatic habitats or aeroterrestrial algae also could be good candidates of research studying salt
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Synergistic effects of rhizosphere effect and combined organic and chemical fertilizers application on soil bacterial diversity and community structure in oilseed rape cultivation Front. Microbiol. (IF 5.2) Pub Date : 2024-03-14 Jingyuan Wang, Hongling Qin, Leyan Zhang, Yafang Tang, Junjiang Long, Huaqin Xu, Baoli Zhu
The combined application of chemical and organic fertilizers has been recognized to enhance soil fertility and foster the soil microbial ecosystem. However, the optimal ratio of chemical and organic fertilizers in oilseed rape cultivation is still uncertain, and the role of rhizosphere effect is still unclear. Thus, this study aimed to elucidate the impacts of varying ratios of chemical and organic
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Long-term saline water irrigation affected soil carbon and nitrogen cycling functional profiles in the cotton field Front. Microbiol. (IF 5.2) Pub Date : 2024-03-14 Shuang Zhou, Guangshuai Wang, Qisheng Han, Junpeng Zhang, Hongkai Dang, Huifeng Ning, Yang Gao, Jingsheng Sun
Saline water irrigation (SWI) plays an important role in alleviating water resource shortages. At the same time, the salt input of irrigation water affects soil microorganisms which participate in various ecological processes of terrestrial ecosystems. However, the responses of soil microbial functional potential to long-term SWI remains unclear. Therefore, Metagenomics method was utilized in cotton
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Molecular characterization of hybrid virulence plasmids in ST11-KL64 KPC-2-producing multidrug-resistant hypervirulent Klebsiella pneumoniae from China Front. Microbiol. (IF 5.2) Pub Date : 2024-03-14 Fushan Zhang, Leyuan Li, Yuxin Zhao, Huiyue Dong, Buhui Zhao, Xiaoyu Zhao, Ziwei Xia, Leizi Chi, Yan Wang, Ruichao Li, Shangshang Qin, Xiangjing Fu
IntroductionCarbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) strains combining virulence and multidrug resistance (MDR) features pose a great public health concern. The aim of this study is to explore the evolutionary characteristics of virulence in CR-HvKP by investigating the genetic features of resistance and virulence hybrid plasmids.MethodsThe resistance and virulence phenotypes
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Transcriptome and metabolome analysis reveals PRV XJ delgE/gI/TK protects intracranially infected mice from death by regulating the inflammation Front. Microbiol. (IF 5.2) Pub Date : 2024-03-14 Lei Xu, Yang Zhang, Qian Tao, Tong Xu, Feng-qin Lee, Li-shuang Deng, Zhijie Jian, Jun Zhao, Yanting Yang, Siyuan Lai, Yuan-cheng Zhou, Zhi-wen Xu, Ling Zhu
Pseudorabies virus can cause inflammation in the central nervous system and neurological symptoms. To further investigate the protective mechanism of PRV XJ delgE/gI/TK in the central nervous system, an intracranial PRV-infection mice model was developed. The results demonstrated that immunization with PRV XJ delgE/gI/TK successfully prevented death caused by PRV-intracranial infection. Subsequently
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Investigation of porcine circovirus type 2 and porcine circovirus type 3 infections based on dual TaqMan fluorescent quantitative PCR method and genetic evolutionary analysis of these two viruses Front. Microbiol. (IF 5.2) Pub Date : 2024-03-14 Mengxiang Cao, Yanwu Wei, Weilin Shi, Li Feng, Liping Huang
IntroductionPorcine circovirus type 2 (PCV2) is the pathogen of Porcine Circovirus Associated Diseases. Porcine circovirus type 3 (PCV3) is a novel porcine circovirus associated with porcine dermatitis and nephropathy syndrome (PDNS) and reproductive failure. PCV2 is clearly pathogenic, while the pathogenicity of PCV3 remains controversial, so it is crucial to monitor the prevalence of PCV2 and PCV3
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The diversity of endophytic fungi in Tartary buckwheat (Fagopyrum tataricum) and its correlation with flavonoids and phenotypic traits Front. Microbiol. (IF 5.2) Pub Date : 2024-03-14 Meiqi Chen, Ziqi Ding, Min Zhou, Yukun Shang, Chenglei Li, Qingfeng Li, Tongliang Bu, Zizhong Tang, Hui Chen
Tartary buckwheat (Fagopyrum tataricum) is a significant medicinal crop, with flavonoids serving as a crucial measure of its quality. Presently, the artificial cultivation of Tartary buckwheat yields low results, and the quality varies across different origins. Therefore, it is imperative to identify an effective method to enhance the yield and quality of buckwheat. Endophytic fungi reside within plants
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The roles of SARP family regulators involved in secondary metabolism in Streptomyces Front. Microbiol. (IF 5.2) Pub Date : 2024-03-14 Yusi Yan, Haiyang Xia
Streptomyces species are best known for their ability to produce abundant secondary metabolites with versatile bioactivities and industrial importance. These metabolites are usually biosynthesized through metabolic pathways encoded by cluster-situated genes. These genes are also known as biosynthetic gene clusters (BGCs) of secondary metabolites. The expression of BGCs is intricately controlled by
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Construction of cellulose-degrading microbial consortium and evaluation of their ability to degrade spent mushroom substrate Front. Microbiol. (IF 5.2) Pub Date : 2024-03-14 Junqiao Long, Xueli Wang, Shuyi Qiu, Wei Zhou, Shaoqi Zhou, Kaiwei Shen, Lili Xie, Xiao Ma, Xuyi Zhang
IntroductionSpent mushroom substrate (SMS) is a solid waste in agricultural production that contains abundant lignocellulosic fibers. The indiscriminate disposal of SMS will lead to significant resource waste and pollution of the surrounding environment.The isolation and screening of microorganisms with high cellulase degradation capacity is the key to improving SMS utilization.MethodsThe cellulose-degrading
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Soil nitrogen availability and microbial carbon use efficiency are dependent more on chemical fertilization than winter drought in a maize–soybean rotation system Front. Microbiol. (IF 5.2) Pub Date : 2024-03-14 Wenqing Bao, Peng He, Lin Han, Xiaowei Wei, Lei Feng, Jianqin Zhu, Jihua Wang, Xuechen Yang, Lu-Jun Li
Soil nitrogen (N) availability is one of the limiting factors of crop productivity, and it is strongly influenced by global change and agricultural management practices. However, very few studies have assessed how the winter drought affected soil N availability during the subsequent growing season under chemical fertilization. We conducted a field investigation involving snow removal to simulate winter
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SARS-CoV-2 mutant spectra as variant of concern nurseries: endless variation? Front. Microbiol. (IF 5.2) Pub Date : 2024-03-14 Brenda Martínez-González, María Eugenia Soria, Pablo Mínguez, Ramón Lorenzo-Redondo, Llanos Salar-Vidal, Alberto López-García, Mario Esteban-Muñoz, Antoni Durán-Pastor, Pilar Somovilla, Carlos García-Crespo, Ana Isabel de Ávila, Jordi Gómez, Jaime Esteban, Ricardo Fernández-Roblas, Ignacio Gadea, Esteban Domingo, Celia Perales
IntroductionSARS-CoV-2 isolates of a given clade may contain low frequency genomes that encode amino acids or deletions which are typical of a different clade.MethodsHere we use high resolution ultra-deep sequencing to analyze SARS-CoV-2 mutant spectra.ResultsIn 6 out of 11 SARS-CoV-2 isolates from COVID-19 patients, the mutant spectrum of the spike (S)-coding region included two or more amino acids
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Microbiome markers in HPV-positive and HPV-negative women of reproductive age with ASCUS and SIL determined by V4 region of 16S rRNA gene sequencing Front. Microbiol. (IF 5.2) Pub Date : 2024-03-14 Anastasiya Peremykina, Valery Cheranev, Andrey Krivoy, Alexander O. Andreev, Zhanna Repinskaia, Aleksandra V. Asaturova, Dmitriy Korostin, Denis Rebrikov, Gyuldana R. Bayramova
IntroductionHuman papilloma virus (HPV) is the most common sexually transmitted infection worldwide. Cervicovaginal microbiota plays an important role in HPV infection and is associated with the development of squamous intraepithelial lesions (SIL). The natural history of cervical cancer involves reversible changes in the cervical tissue from a normal state, in which no neoplastic changes are detected
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Comprehensive genome sequence analysis of Ralstonia solanacearum gd-2, a phylotype I sequevar 15 strain collected from a tobacco bacterial phytopathogen Front. Microbiol. (IF 5.2) Pub Date : 2024-03-14 Zhiliang Xiao, Guangcan Li, Aiguo Yang, Zhengwen Liu, Min Ren, Lirui Cheng, Dan Liu, Caihong Jiang, Liuying Wen, Shengxin Wu, Yazhi Cheng, Wen Yu, Ruimei Geng
IntroductionPlant bacterial wilt is an important worldwide disease caused by Ralstonia solanacearum which is a complex of species.MethodsIn this study, we identified and sequenced the genome of R. solanacearum strain gd-2 isolated from tobacco.ResultsStrain gd-2 was identified as R. solanacearum species complex (RSSC) phylotype I sequevar 15 and exhibited strong pathogenicity to tobacco. The genome
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Research reviews and prospects of gut microbiota in liver cirrhosis: a bibliometric analysis (2001–2023) Front. Microbiol. (IF 5.2) Pub Date : 2024-03-14 Xiaofei Zhu, Ziyuan Zhou, Xiaxia Pan
IntroductionThe gut-liver axis has emerged as a focal point in chronic liver disorders, prompting more research into the role of the gut microbiota in liver cirrhosis. In individuals with liver cirrhosis, changes in the structure and function of the gut microbiota are closely tied to clinical prognosis. However, there is a scarcity of bibliometric evaluations conducted in this particular field.MethodsThis
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Application of photoelectrochemical oxidation of wastewater used in the cooling tower water and its influence on microbial corrosion Front. Microbiol. (IF 5.2) Pub Date : 2024-03-13 Seenivasan Kokilaramani, Alagersamy Satheeshkumar, M. S. Nandini, Jayaraman Narenkumar, Mohamad S. AlSalhi, Sandhanasamy Devanesan, Prabhu Manickam Natarajan, Rajaram Rajamohan, Aruliah Rajasekar, Tabarak Malik
BackgroundCooling towers are specialized heat exchanger devices in which air and water interact closely to cool the water's temperature. However, the cooling water contains organic nutrients that can cause microbial corrosion (MC) on the metal surfaces of the tower. This research explores the combined wastewater treatment approach using electrochemical-oxidation (EO), photo-oxidation (PO), and photoelectrochemical
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Beneficial effects of American ginseng (Panax quinquefolius L.) extract residue as a feed additive on production, health status, and gastrointestinal bacteria in sika deer (Cervus nippon) Front. Microbiol. (IF 5.2) Pub Date : 2024-03-13 Yan Wu, Shuting Zhao, Peihe Zheng, Hanlu Liu, Zhengyi Qu, Wei Hou, Weitao Yuan, Tao Feng, Xiaofeng Zhan, Jinlong Shen, Kaiying Wang
American ginseng residue is an industrial by-product of ginseng saponin extraction, including polysaccharides and amino acids; however, it is often discarded into the natural environment, representing a waste of resources as well as an environmental issue. In this study, we examined the effects of adding American ginseng residue to the basal diet of sika deer. Twelve antler-bearing male sika deer were
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Interactions between halotolerant nitrogen-fixing bacteria and arbuscular mycorrhizal fungi under saline stress Front. Microbiol. (IF 5.2) Pub Date : 2024-03-13 Chao Ji, Yuhan Ge, Hua Zhang, Yingxiang Zhang, Zhiwen Xin, Jian Li, Jinghe Zheng, Zengwen Liang, Hui Cao, Kun Li
Background and aimsSoil salinity negatively affects crop development. Halotolerant nitrogen-fixing bacteria (HNFB) and arbuscular mycorrhizal fungi (AMF) are essential microorganisms that enhance crop nutrient availability and salt tolerance in saline soils. Studying the impact of HNFB on AMF communities and using HNFB in biofertilizers can help in selecting the optimal HNFB-AMF combinations to improve
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The alternative coproporphyrinogen III oxidase (CgoN) catalyzes the oxygen-independent conversion of coproporphyrinogen III into coproporphyrin III Front. Microbiol. (IF 5.2) Pub Date : 2024-03-13 Toni Mingers, Stefan Barthels, Violetta Mass, José Manuel Borrero-de Acuña, Rebekka Biedendieck, Ana Cooke, Tamara A. Dailey, Svetlana Gerdes, Wulf Blankenfeldt, Harry A. Dailey, Martin J. Warren, Martina Jahn, Dieter Jahn
Nature utilizes three distinct pathways to synthesize the essential enzyme cofactor heme. The coproporphyrin III-dependent pathway, predominantly present in Bacillaceae, employs an oxygen-dependent coproporphyrinogen III oxidase (CgoX) that converts coproporphyrinogen III into coproporphyrin III. In this study, we report the bioinformatic-based identification of a gene called ytpQ, encoding a putative
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Targeted cultivation of diatoms in mariculture wastewater by nutrient regulation and UV-C irradiation Front. Microbiol. (IF 5.2) Pub Date : 2024-03-13 Jiacong Shen, Xiafei Zheng, Minhai Liu, Kui Xu, Lin He, Zhihua Lin
Mariculture wastewater poses environmental challenges due to pollution and eutrophication. Targeted cultivation of diatoms in wastewater can help alleviate these issues while generating beneficial algae biomass, however reliable operating methods are lacking. We proposed a novel method for treating mariculture wastewater that employed UV-C irradiation and nutrient regulation to achieve targeted diatom
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A multiplex TaqMan real-time PCR assays for the rapid detection of mobile colistin resistance (mcr-1 to mcr-10) genes Front. Microbiol. (IF 5.2) Pub Date : 2024-03-13 Xinran Gong, Guang Yang, Wei Liu, Di Wu, Chunyuan Duan, Xinjing Jia, Zhiqiang Li, Xiaocang Zou, Renfeng Yu, Dayang Zou, Yong Wang
ObjectiveRecently, 10 plasmid-mediated mobile colistin resistance genes, mcr-1 to mcr-10, and their variants have been identified, posing a new threat to the treatment of clinical infections caused by Gram-negative bacteria. Our objective was to develop a rapid, sensitive, and accurate molecular assay for detecting mcr genes in clinical isolates.MethodsThe primers and corresponding TaqMan-MGB probes
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Mapping the tRNA modification landscape of Bartonella henselae Houston I and Bartonella quintana Toulouse Front. Microbiol. (IF 5.2) Pub Date : 2024-03-13 Samia Quaiyum, Jingjing Sun, Virginie Marchand, Guangxin Sun, Colbie J. Reed, Yuri Motorin, Peter C. Dedon, Michael F. Minnick, Valérie de Crécy-Lagard
Transfer RNA (tRNA) modifications play a crucial role in maintaining translational fidelity and efficiency, and they may function as regulatory elements in stress response and virulence. Despite their pivotal roles, a comprehensive mapping of tRNA modifications and their associated synthesis genes is still limited, with a predominant focus on free-living bacteria. In this study, we employed a multidisciplinary
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Preventing microbe colonization on avocado (Persea nubigena var. guatemalensis) through metabiotic treatment, a promising postharvest safety improvement Front. Microbiol. (IF 5.2) Pub Date : 2024-03-13 Gabriela N. Tenea, Evelyn Angamarca, Victor Cifuentes, Jazmin Hidalgo
IntroductionLactic acid bacteria (LAB) produce various metabolites (i.e. metabiotics) with inhibitory capacity towards harmful foodborne pathogens.MethodsThis study aimed to design several antimicrobial formulations based on metabiotics obtained from different native LAB species (Lactobacillus pentosus UTNGt5, Lactococcus lactis UTNGt28, and Weissella cibaria UTNGt21O) and to detect the possible mode
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Desirable L-asparaginases for treating cancer and current research trends Front. Microbiol. (IF 5.2) Pub Date : 2024-03-13 Kindu Tsegaye, Berhanu Andualem Tsehai, Birhan Getie
Amino acid depletion therapy is a promising approach for cancer treatment. It exploits the differences in the metabolic processes between healthy and cancerous cells. Certain microbial enzymes induce cancer cell apoptosis by removing essential amino acids. L-asparaginase is an enzyme approved by the FDA for the treatment of acute lymphoblastic leukemia. The enzymes currently employed in clinics come
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Exploring the microbial ecosystem of Berchemia polyphylla var. leioclada: a comprehensive analysis of endophytes and rhizospheric soil microorganisms Front. Microbiol. (IF 5.2) Pub Date : 2024-03-13 Yuanjiang Tang, Sixuan Zhou, Yuanpin Xiao, Tao Zhang, Xiaoyan Tao, Kaizhi Shi, Yuxi Lu, Yueqian Yang, Yu Zhao, Tian Zhao
Endophytic and rhizospheric microorganisms associated with plants play a crucial role in plant health, pest and disease defense, and fruit yield by actively participating in the plant’s adaptation to its environment. In this study, high–throughput sequencing technology was employed to analyze the community structure and diversity of endophytic and rhizospheric soil microorganisms in Berchemia polyphylla
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Responses of soil bacterial communities and maize yields to sulfur application across four soil types Front. Microbiol. (IF 5.2) Pub Date : 2024-03-13 Siqi Dong, Bing Zhang, Zhao Wang, Xue Zhou, Qiang Gao
IntroductionThis study assessed the effects of S application on maize yields and soil bacterial communities across four sites with different soil types and three S application rates (0 kg ha-1, 30 kg ha-1, and 90 kg ha-1).MethodsChanges in soil properties, bacterial community diversity, structure, and their contributions to maize production were evaluated post-S application treatments.Results(1) S
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Effects of aeration modes and rates on nitrogen conversion and bacterial community in composting of dehydrated sludge and corn straw Front. Microbiol. (IF 5.2) Pub Date : 2024-03-12 Yuyun Wang, PengXiang Xu, Yue Wang, Jing Su, Zhi Xu, Zhengbo Jiang, Yuquan Wei, Sheng Hang, Xiaoyan Ding, Hao Zhang, Longli Zhang, Yongdi Liu, Ji Li
Aeration is an important factor to regulate composting efficiency and nitrogen loss. This study is aimed to compare the effects of different aeration modes (continuous and intermittent) and aeration rate on nitrogen conversion and bacterial community in composting from dehydrated sludge and corn straw. Results showed that the intermittent aeration mode at same aeration volume was superior to the continuous
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Biological characteristics of the bacteriophage LDT325 and its potential application against the plant pathogen Pseudomonas syringae Front. Microbiol. (IF 5.2) Pub Date : 2024-03-12 Li Liu, Bing Wang, Anqi Huang, Hua Zhang, Yubao Li, Lei Wang
Bud blight disease caused by Pseudomonas syringae is a major bacterial disease of tea plants in China. Concerns regarding the emergence of bacterial resistance to conventional copper controls have indicated the need to devise new methods of disease biocontrol. Phage-based biocontrol may be a sustainable approach to combat bacterial pathogens. In this study, a P. syringae phage was isolated from soil
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Integrating transcriptomics and metabolomics to analyze the defense response of Morus notabilis to mulberry ring rot disease Front. Microbiol. (IF 5.2) Pub Date : 2024-03-12 Qianqian Qian, Xinqi Deng, Sumbul Mureed, Yujie Gan, Danping Xu, Xie Wang, Habib Ali
IntroductionThe mulberry industry has thrived in China for millennia, offering significant ecological and economic benefits. However, the prevalence of mulberry ring rot disease poses a serious threat to the quality and yield of mulberry leaves.MethodsIn this study, we employed a combination of transcriptomic and metabolomic analyses to elucidate the changes occurring at the transcriptional and metabolic
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Maintaining the native gut microbiota of bharal (Pseudois nayaur) is crucial in ex situ conservation Front. Microbiol. (IF 5.2) Pub Date : 2024-03-12 Hongmei Gao, Xiangwen Chi, Pengfei Song, Haifeng Gu, Bo Xu, Zhenyuan Cai, Feng Jiang, Bin Li, Tongzuo Zhang
As wildlife protection continue to strengthen, research on the gut microbiota of wildlife is increasing. Carrying out conservation and research on endangered species in the Qinghai Tibet Plateau plays an important role in global biodiversity conservation. This study utilized 16S rRNA sequencing of fecal samples to investigate the composition, function, and changes of the gut microbiota of bharal in
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Evaluation of the control efficacy of antagonistic bacteria from V-Ti magnetite mine tailings on kiwifruit brown spots in pot and field experiments Front. Microbiol. (IF 5.2) Pub Date : 2024-03-12 Yongliang Cui, Yuhang Zhu, Guanyong Dong, Yanmei Li, Jing Xu, Zuqiang Cheng, Lijun Li, Guoshu Gong, Xiumei Yu
Seemingly barren heavy-metal-polluted vanadium (V) and titanium (Ti) magnetite mine tailings contain various functional microbes, yet it is unclear whether this includes microbial resources relevant to the biological control of plant diseases. Kiwifruit brown leaf spot disease, caused by Corynespora cassiicola, can seriously reduce kiwifruit yield. To discover effective control measures for kiwifruit
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Changes in the bacterial communities of Harmonia axyridis (Coleoptera: Coccinellidae) in response to long-term cold storage and progressive loss of egg viability in cold-stored beetles Front. Microbiol. (IF 5.2) Pub Date : 2024-03-12 Yuanxing Sun, Yanan Hao, Senshan Wang, Xinling Chen
Bacteria have a profound influence on life history and reproduction of numerous insects, while the associations between hosts and bacteria are substantially influenced by environmental pressures. Cold storage is crucial for extending the shelf life of insects used as tools for biological control, but mostly causes detrimental effects. In this study, we observed a great decrease in egg hatch rate of
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A single point mutation in the Listeria monocytogenes ribosomal gene rpsU enables SigB activation independently of the stressosome and the anti-sigma factor antagonist RsbV Front. Microbiol. (IF 5.2) Pub Date : 2024-03-12 Xuchuan Ma, Marcel H. Tempelaars, Marcel H. Zwietering, Sjef Boeren, Conor P. O’Byrne, Heidy M. W. den Besten, Tjakko Abee
Microbial population heterogeneity leads to different stress responses and growth behavior of individual cells in a population. Previously, a point mutation in the rpsU gene (rpsUG50C) encoding ribosomal protein S21 was identified in a Listeria monocytogenes LO28 variant, which leads to increased multi-stress resistance and a reduced maximum specific growth rate. However, the underlying mechanisms
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Endophytic Aspergillus hiratsukae mediated biosynthesis of silver nanoparticles and their antimicrobial and photocatalytic activities Front. Microbiol. (IF 5.2) Pub Date : 2024-03-12 Ebrahim Saied, Mostafa A. Abdel-Maksoud, Akram A. Alfuraydi, Bushra Hafeez Kiani, Mohamed Bassyouni, Osama A. Al-Qabandi, Fathia H. E. Bougafa, Mona Shaban E. M. Badawy, Amr H. Hashem
In the current study, endophytic Aspergillus hiratsukae was used for the biosynthesis of silver nanoparticles (Ag-NPs) for the first time. The characterizations were performed using X ray diffraction (XRD), Transmission electron microscopy (TEM), Scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM–EDX), Dynamic light scattering (DLS), Fourier transform infrared spectroscopy
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Zero budget natural farming components Jeevamrit and Beejamrit augment Spinacia oleracea L. (spinach) growth by ameliorating the negative impacts of the salt and drought stress Front. Microbiol. (IF 5.2) Pub Date : 2024-03-12 Margi Patel, Shaikhul Islam, Bernard R. Glick, Nisha Choudhary, Virendra Kumar Yadav, Snehal Bagatharia, Dipak Kumar Sahoo, Ashish Patel
The growth of crop plants, particularly spinach (Spinacia oleracea L.), can be significantly impeded by salinity and drought. However, pre-treating spinach plants with traditional biofertilizers like Jeevamrit and Beejamrit (JB) substantially reverses the salinity and drought-induced inhibitory effects. Hence, this study aims to elucidate the underlying mechanisms that govern the efficacy of traditional
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Super resolution-based methodology for self-supervised segmentation of microscopy images Front. Microbiol. (IF 5.2) Pub Date : 2024-03-12 Vidya Bommanapally, Dilanga Abeyrathna, Parvathi Chundi, Mahadevan Subramaniam
Data-driven Artificial Intelligence (AI)/Machine learning (ML) image analysis approaches have gained a lot of momentum in analyzing microscopy images in bioengineering, biotechnology, and medicine. The success of these approaches crucially relies on the availability of high-quality microscopy images, which is often a challenge due to the diverse experimental conditions and modes under which these images
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Natronoglomus mannanivorans gen. nov., sp. nov., beta-1,4-mannan utilizing natronoarchaea from hypersaline soda lakes Front. Microbiol. (IF 5.2) Pub Date : 2024-03-12 Dimitry Y. Sorokin, Alexander G. Elcheninov, Nicole J. Bale, Jaap S. Sinninghe Damsté, Ilya V. Kublanov
Beta-mannans are insoluble plant polysaccharides with beta-1,4-linked mannose as the backbone. We used three forms of this polysaccharide, namely, pure mannan, glucomannan, and galactomannan, to enrich haloarchaea, which have the ability to utilize mannans for growth. Four mannan-utilizing strains obtained in pure cultures were closely related to each other on the level of the same species. Furthermore
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Protective potential of outer membrane vesicles derived from a virulent strain of Francisella tularensis Front. Microbiol. (IF 5.2) Pub Date : 2024-03-12 Ivona Pavkova, Jan Bavlovic, Klara Kubelkova, Jiri Stulik, Jana Klimentova
Francisella tularensis secretes tubular outer membrane vesicles (OMVs) that contain a number of immunoreactive proteins as well as virulence factors. We have reported previously that isolated Francisella OMVs enter macrophages, cumulate inside, and induce a strong pro-inflammatory response. In the current article, we present that OMVs treatment of macrophages also enhances phagocytosis of the bacteria
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Development and validation of a random forest algorithm for source attribution of animal and human Salmonella Typhimurium and monophasic variants of S. Typhimurium isolates in England and Wales utilising whole genome sequencing data Front. Microbiol. (IF 5.2) Pub Date : 2024-03-12 Jaromir Guzinski, Yue Tang, Marie Anne Chattaway, Timothy J. Dallman, Liljana Petrovska
Source attribution has traditionally involved combining epidemiological data with different pathogen characterisation methods, including 7-gene multi locus sequence typing (MLST) or serotyping, however, these approaches have limited resolution. In contrast, whole genome sequencing data provide an overview of the whole genome that can be used by attribution algorithms. Here, we applied a random forest
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Metagenomic sequencing identified microbial species in the rumen and cecum microbiome responsible for niacin treatment and related to intramuscular fat content in finishing cattle Front. Microbiol. (IF 5.2) Pub Date : 2024-03-11 Zhuqing Yang, Xiao Chen, Mingjin Yu, Ruixue Jing, Linbin Bao, Xianghui Zhao, Ke Pan, Bihui Chao, Mingren Qu
IntroductionNiacin is one of the essential vitamins for mammals. It plays important roles in maintaining rumen microecological homeostasis. Our previous study indicated that dietary niacin significantly elevated intramuscular fat content (IMF) in castrated finishing steers. Whether niacin affects fat deposition by regulating the microbial composition and functional capacities of gastrointestinal microbiome
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Microbial diversity in camel milk from Xinjiang, China as revealed by metataxonomic analysis Front. Microbiol. (IF 5.2) Pub Date : 2024-03-11 Miao Sun, Wei Shao, Zhengyu Liu, Xianlan Ma, He Chen, Nan Zheng, Yankun Zhao
The quality of raw camel milk is affected by its bacterial composition and diversity. However, few studies have investigated the bacterial composition and diversity of raw camel milk. In this study, we obtained 20 samples of camel milk during spring and summer in Urumqi and Hami, Xinjiang, China. Single-molecule real-time sequencing technology was used to analyze the bacterial community composition
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Epidemiological investigation and pathogenicity analysis of waterfowl astroviruses in some areas of China Front. Microbiol. (IF 5.2) Pub Date : 2024-03-11 Yang Li, Juan Luo, Jiajing Shang, Fuyou Zhang, Chunran Deng, Yingjie Feng, Ge Meng, Wenming Jiang, Xiaohui Yu, Hualei Liu
Waterfowl astroviruses are mainly duck astroviruses and goose astroviruses, of which duck astroviruses (DAstV-3, -4), goose astroviruses (GoAstV-1, -2) are the four new waterfowl 21 astroviruses in recent years, which can lead to enteritis, viral hepatitis, gout and reduce the growth performance of waterfowl, affecting the healthy development of the waterfowl farming industry. Since no targeted drugs