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Long-term push–pull cropping system shifts soil and maize-root microbiome diversity paving way to resilient farming system BMC Microbiol. (IF 4.2) Pub Date : 2024-03-18 Abdul A. Jalloh, Fathiya Mbarak Khamis, Abdullahi Ahmed Yusuf, Sevgan Subramanian, Daniel Munyao Mutyambai
The soil biota consists of a complex assembly of microbial communities and other organisms that vary significantly across farming systems, impacting soil health and plant productivity. Despite its importance, there has been limited exploration of how different cropping systems influence soil and plant root microbiomes. In this study, we investigated soil physicochemical properties, along with soil
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Pretreatment with an antibiotics cocktail enhances the protective effect of probiotics by regulating SCFA metabolism and Th1/Th2/Th17 cell immune responses BMC Microbiol. (IF 4.2) Pub Date : 2024-03-18 Jing Xu, Haoming Xu, Xue Guo, Hailan Zhao, Jiaqi Wang, Jianhong Li, Jie He, Hongli Huang, Chen Huang, Chong Zhao, Yingfei Li, Youlian Zhou, Yao Peng, Yuqiang Nie
Probiotics are a potentially effective therapy for inflammatory bowel disease (IBD); IBD is linked to impaired gut microbiota and intestinal immunity. However, the utilization of an antibiotic cocktail (Abx) prior to the probiotic intervention remains controversial. This study aims to identify the effect of Abx pretreatment from dextran sulfate sodium (DSS)-induced colitis and to evaluate whether Abx
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High-throughput sequencing reveals differences in microbial community structure and diversity in the conjunctival tissue of healthy and type 2 diabetic mice BMC Microbiol. (IF 4.2) Pub Date : 2024-03-16 Fengjiao Li, Shuo Yang, Ji Ma, Xiaowen Zhao, Meng Chen, Ye Wang
To investigate the differences in bacterial and fungal community structure and diversity in conjunctival tissue of healthy and diabetic mice. RNA-seq assays and high-throughput sequencing of bacterial 16 S rDNA and fungal internal transcribed spacer (ITS) gene sequences were used to identify differentially expressed host genes and fungal composition profiles in conjunctival tissues of diabetic BKS-db/db
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High prevalence of ST5-SCCmec II-t311 clone of methicillin-resistant Staphylococcus aureus isolated from bloodstream infections in East China BMC Microbiol. (IF 4.2) Pub Date : 2024-03-16 Qing Zhan, Gaoqin Teng, Weiwei Chen, Xiao Yu
Methicillin-resistant Staphylococcus aureus (MRSA) is a challenging global health threat, resulting in significant morbidity and mortality worldwide. This study aims to determine the molecular characteristics and antimicrobial susceptibility of 263 MRSA isolates in Zhejiang Province, east China. From 2014 to 2019, a total of 263 MRSA isolates from bloodstream infections (BSIs) were collected from 6
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Characteristics of the oral and gastric microbiome in patients with early-stage intramucosal esophageal squamous cell carcinoma BMC Microbiol. (IF 4.2) Pub Date : 2024-03-15 Han Chen, Xingzhou Jiang, Fengyi Zhu, Ruoyun Yang, Xin Yu, Xiaoying Zhou, Nana Tang
Oral microbiome dysbacteriosis has been reported to be associated with the pathogenesis of advanced esophageal cancer. However, few studies investigated the potential role of oral and gastric microbiota in early-stage intramucosal esophageal squamous carcinoma (EIESC). A total of 104 samples were collected from 31 patients with EIESC and 21 healthy controls. The compositions of oral and gastric microbiota
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The potential role of Listeria monocytogenes in promoting colorectal adenocarcinoma tumorigenic process BMC Microbiol. (IF 4.2) Pub Date : 2024-03-15 Giulia Baldelli, Mauro De Santi, Collins Njie Ateba, Giorgia Cifola, Giulia Amagliani, Christ-Donald Kaptchouang Tchatchouang, Peter Kotsoana Montso, Giorgio Brandi, Giuditta Fiorella Schiavano
Listeria monocytogenes is a foodborne pathogen, which can cause a severe illness, especially in people with a weakened immune system or comorbidities. The interactions between host and pathogens and between pathogens and tumor cells have been debated in recent years. However, it is still unclear how bacteria can interact with tumor cells, and if this interaction can affect tumor progression and therapy
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Evaluation of clinical characteristics and risk factors associated with Chlamydia psittaci infection based on metagenomic next-generation sequencing BMC Microbiol. (IF 4.2) Pub Date : 2024-03-13 Lei Yuan, Qiang Chen, Xin Yu Zhu, Lan Min Lai, Rui Zhao, Yang Liu
Psittacosis is a zoonosis caused by Chlamydia psittaci, the clinical manifestations of Psittacosis range from mild illness to fulminant severe pneumonia with multiple organ failure. This study aimed to evaluate the clinical characteristics of Chlamydia psittaci infection diagnosed based on metagenomic next-generation sequencing(mNGS), as well as the risk factors affecting the progress of Chlamydia
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Characterization of the broad-spectrum antibacterial activity of bacteriocin-like inhibitory substance-producing probiotics isolated from fermented foods BMC Microbiol. (IF 4.2) Pub Date : 2024-03-11 Tran Thi Dieu Thuy, Hsu-Feng Lu, Carl Jay Ballena Bregente, Fong-Chi Annabelle Huang, Pei-Chun Tu, Cheng-Yen Kao
Antimicrobial peptides, such as bacteriocin, produced by probiotics have become a promising novel class of therapeutic agents for treating infectious diseases. Selected lactic acid bacteria (LAB) isolated from fermented foods with probiotic potential were evaluated for various tests, including exopolysaccharide production, antibiotic susceptibility, acid and bile tolerance, antibacterial activity,
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Metagenomic gut microbiome analysis of Japanese patients with multiple chemical sensitivity/idiopathic environmental intolerance BMC Microbiol. (IF 4.2) Pub Date : 2024-03-11 Kentaro Watai, Wataru Suda, Rina Kurokawa, Kiyoshi Sekiya, Hiroaki Hayashi, Maki Iwata, Kisako Nagayama, Yuto Nakamura, Yuto Hamada, Yosuke Kamide, Yuma Fukutomi, Takeru Nakabayashi, Kosei Tanaka, Masahiro Kamita, Masami Taniguchi, Masahira Hattori
Although the pathology of multiple chemical sensitivity (MCS) is unknown, the central nervous system is reportedly involved. The gut microbiota is important in modifying central nervous system diseases. However, the relationship between the gut microbiota and MCS remains unclear. This study aimed to identify gut microbiota variations associated with MCS using shotgun metagenomic sequencing of fecal
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The effect of in vitro simulated colonic pH gradients on microbial activity and metabolite production using common prebiotics as substrates BMC Microbiol. (IF 4.2) Pub Date : 2024-03-11 Zhuqing Xie, Weiwei He, Alex Gobbi, Hanne Christine Bertram, Dennis Sandris Nielsen
The interplay between gut microbiota (GM) and the metabolization of dietary components leading to the production of short-chain fatty acids (SCFAs) is affected by a range of factors including colonic pH and carbohydrate source. However, there is still only limited knowledge on how the GM activity and metabolite production in the gastrointestinal tract could be influenced by pH and the pH gradient increases
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Gut microbial network signatures of early colonizers in preterm neonates with extrauterine growth restriction BMC Microbiol. (IF 4.2) Pub Date : 2024-03-09 Yumei Liang, Xiaomin Yao, Zida Meng, Jinyun Lan, Yanqing Qiu, Chao Cen, Yanni Feng
Extrauterine growth restriction (EUGR) represents a prevalent condition observed in preterm neonates, which poses potential adverse implications for both neonatal development and long-term health outcomes. The manifestation of EUGR has been intricately associated with perturbations in microbial and metabolic profiles. This study aimed to investigate the characteristics of the gut microbial network
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Early transcriptional changes of heavy metal resistance and multiple efflux genes in Xanthomonas campestris pv. campestris under copper and heavy metal ion stress BMC Microbiol. (IF 4.2) Pub Date : 2024-03-09 Stephen D. B. Ramnarine, Omar Ali, Jayaraj Jayaraman, Adesh Ramsubhag
Copper-induced gene expression in Xanthomonas campestris pv. campestris (Xcc) is typically evaluated using targeted approaches involving qPCR. The global response to copper stress in Xcc and resistance to metal induced damage is not well understood. However, homologs of heavy metal efflux genes from the related Stenotrophomonas genus are found in Xanthomonas which suggests that metal related efflux
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Whole-genome sequencing and analysis of Chryseobacterium arthrosphaerae from Rana nigromaculata BMC Microbiol. (IF 4.2) Pub Date : 2024-03-08 Lihong Zhu, Hao Liu, Xiaohui Li, Yuefeng Shi, Xiaoliang Yin, Xionge Pi
Chryseobacterium arthrosphaerae strain FS91703 was isolated from Rana nigromaculata in our previous study. To investigate the genomic characteristics, pathogenicity-related genes, antimicrobial resistance, and phylogenetic relationship of this strain, PacBio RS II and Illumina HiSeq 2000 platforms were used for the whole genome sequencing. The genome size of strain FS91703 was 5,435,691 bp and GC content
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Changes in the nasopharyngeal and oropharyngeal microbiota in pediatric obstructive sleep apnea before and after surgery: a prospective study BMC Microbiol. (IF 4.2) Pub Date : 2024-03-08 Lucheng Fang, Aikebaier Tuohuti, Wanyue Cai, Xiong Chen
To explore the changes and potential mechanisms of microbiome in different parts of the upper airway in the development of pediatric OSA and observe the impact of surgical intervention on oral microbiome for pediatric OSA. Before adeno-tonsillectomy, we collected throat swab samples from different parts of the oropharynx and nasopharynx of 30 OSA patients and 10 non-OSA patients and collected throat
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Unveiling biological activities of biosynthesized starch/silver-selenium nanocomposite using Cladosporium cladosporioides CBS 174.62 BMC Microbiol. (IF 4.2) Pub Date : 2024-03-08 Fathy M. Elkady, Amr H. Hashem, Salem S. Salem, Gharieb S. El-Sayyad, Ahmed Abdel Tawab, Mohammad M. Alkherkhisy, Mohammed S. Abdulrahman
Microbial cells capability to tolerate the effect of various antimicrobial classes represent a major worldwide health concern. The flexible and multi-components nanocomposites have enhanced physicochemical characters with several improved properties. Thus, different biological activities of biosynthesized starch/silver-selenium nanocomposite (St/Ag-Se NC) were assessed. The St/Ag-Se NC was biosynthesized
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Characterization of the major autolysin (AtlC) of Staphylococcus carnosus BMC Microbiol. (IF 4.2) Pub Date : 2024-03-08 Maximilian Merz, Carolin J. Schiffer, Andreas Klingl, Matthias A. Ehrmann
Autolysis by cellular peptidoglycan hydrolases (PGH) is a well-known phenomenon in bacteria. During food fermentation, autolysis of starter cultures can exert an accelerating effect, as described in many studies on cheese ripening. In contrast, very little is known about autolysis of starter cultures used in other fermentations. Staphylococcus (S.) carnosus is often used in raw sausage fermentations
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Exploring Aeromonas dhakensis in Aldabra giant tortoises: a debut report and genetic characterization BMC Microbiol. (IF 4.2) Pub Date : 2024-03-07 Chenxu Zhao, Panpan Qin, Shuai Li, Zilu Chen, Tianliang Wang, Qunchao Liang, Weishi He, Zeyu Peng, Yurong Yang, Zhifeng Peng, Yongtao Li
Aeromonas dhakensis (A. dhakensis) is becoming an emerging pathogen worldwide, with an increasingly significant role in animals and human health. It is a ubiquitous bacteria found in terrestrial and aquatic milieus. However, there have been few reports of reptile infections. In this study, a bacterial strain isolated from a dead Aldabra giant tortoise was identified as A. dhakensis HN-1 through clinical
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Biotransformation of zearalenone to non-estrogenic compounds with two novel recombinant lactonases from Gliocladium BMC Microbiol. (IF 4.2) Pub Date : 2024-03-07 Zongping Sun, Yuting Fang, Yaohuan Zhu, Wen Tian, Junjie Yu, Jun Tang
The mycotoxin zearalenone (ZEA) produced by toxigenic fungi is widely present in cereals and its downstream products. The danger of ZEA linked to various human health issues has attracted increasing attention. Thus, powerful ZEA-degrading or detoxifying strategies are urgently needed. Biology-based detoxification methods are specific, efficient, and environmentally friendly and do not lead to negative
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Multidrug resistance among uropathogenic clonal group A E. Coli isolates from Pakistani women with uncomplicated urinary tract infections BMC Microbiol. (IF 4.2) Pub Date : 2024-03-07 Ayesha Khan, Viqar Sayeed Saraf, Fariha Siddiqui, Tahira Batool, Zobia Noreen, Sundus Javed, Aftab Ahmad, Wadi B. Alonazi, Muhammad Ibrahim, Sandra Pucciarelli, Habib Bokhari
Multi-drug resistance (MDR) has notably increased in community acquired uropathogens causing urinary tract infections (UTIs), predominantly Escherichia coli. Uropathogenic E. coli causes 80% of uncomplicated community acquired UTIs, particularly in pre-menopausal women. Considering this high prevalence and the potential to spread antimicrobial resistant genes, the current study was conducted to investigate
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Uncovering the complexity of childhood undernutrition through strain-level analysis of the gut microbiome BMC Microbiol. (IF 4.2) Pub Date : 2024-03-05 Lili Wang, Wenjie Zhang, Yinan Wang, Yuanzheng Zhang, Shilin Zhong, Peng Gao, Bingmei Chang, Zicheng Zhao
Undernutrition (UN) is a critical public health issue that threatens the lives of children under five in developing countries. While evidence indicates the crucial role of the gut microbiome (GM) in UN pathogenesis, the strain-level inspection and bacterial co-occurrence network investigation in the GM of UN children are lacking. This study examines the strain compositions of the GM in 61 undernutrition
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RpoS role in antibiotic resistance, tolerance and persistence in E. coli natural isolates BMC Microbiol. (IF 4.2) Pub Date : 2024-03-05 Estela Ynés Valencia, Felipe de Moraes Gomes, Katia Ospino, Beny Spira
The intrinsic concentration of RpoS, the second most abundant sigma factor, varies widely across the E. coli species. Bacterial isolates that express high levels of RpoS display high resistance to environmental stresses, such as temperature, pH and osmolarity shifts, but are less nutritional competent, making them less capable of utilising alternative nutrient sources. The role of RpoS in antibiotic
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Fine-scale geographic difference of the endangered Big-headed Turtle (Platysternon megacephalum) fecal microbiota, and comparison with the syntopic Beale’s Eyed Turtle (Sacalia bealei) BMC Microbiol. (IF 4.2) Pub Date : 2024-02-29 Jonathan J. Fong, Yik-Hei Sung, Li Ding
Studies have elucidated the importance of gut microbiota for an organism, but we are still learning about the important influencing factors. Several factors have been identified in helping shape the microbiome of a host, and in this study we focus on two factors—geography and host. We characterize the fecal microbiota of the Big-headed Turtle (Platysternon megacephalum) and compare across a relatively
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The activation impact of lactobacillus-derived extracellular vesicles on lipopolysaccharide-induced microglial cell BMC Microbiol. (IF 4.2) Pub Date : 2024-02-28 Yanfang Yang, Na Li, Yubo Gao, Fanning Xu, Hui Chen, Chun Zhang, Xinli Ni
Perioperative neurocognitive dysfunction (PND) emerges as a common postoperative complication among elderly patients. Currently, the mechanism of PND remains unclear, but there exists a tendency to believe that inflammation plays a significant role in PND. Alterations in the abundance of intestinal microbiota can increase the permeability of the intestinal mucosal barrier and incite extraintestinal
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Development of non-alcoholic steatohepatitis is associated with gut microbiota but not with oxysterol enzymes CH25H, EBI2, or CYP7B1 in mice BMC Microbiol. (IF 4.2) Pub Date : 2024-02-28 Jacqueline Wyss, Tina Raselli, Annika Wyss, Anja Telzerow, Gerhard Rogler, Niklas Krupka, Bahtiyar Yilmaz, Thomas S. B. Schmidt, Benjamin Misselwitz
Liver steatosis is the most frequent liver disorder and its advanced stage, non-alcoholic steatohepatitis (NASH), will soon become the main reason for liver fibrosis and cirrhosis. The “multiple hits hypothesis” suggests that progression from simple steatosis to NASH is triggered by multiple factors including the gut microbiota composition. The Epstein Barr virus induced gene 2 (EBI2) is a receptor
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Rapid, visual, label-based biosensor platform for identification of hepatitis C virus in clinical applications BMC Microbiol. (IF 4.2) Pub Date : 2024-02-28 Yuanfang Shi, Qingxue Zhou, Shilei Dong, Qi Zhao, Xue Wu, Peng Yang, Xiaoyan Zeng, Xinggui Yang, Yan Tan, Xinhua Luo, Zhenghua Xiao, Xu Chen
In the current study, for the first time, we reported a novel HCV molecular diagnostic approach termed reverse transcription loop-mediated isothermal amplification integrated with a gold nanoparticles-based lateral flow biosensor (RT-LAMP-AuNPs-LFB), which we developed for rapid, sensitive, specific, simple, and visual identification of HCV. A set of LAMP primer was designed according to 5’untranslated
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Phenotypic, molecular, and in silico characterization of coumarin as carbapenemase inhibitor to fight carbapenem-resistant Klebsiella pneumoniae BMC Microbiol. (IF 4.2) Pub Date : 2024-02-27 Mahmoud Saad Abdel-Halim, Amira M. El-Ganiny, Basem Mansour, Galal Yahya, Hemat K. Abd El Latif, Momen Askoura
Carbapenems represent the first line treatment of serious infections caused by drug-resistant Klebsiella pneumoniae. Carbapenem-resistant K. pneumoniae (CRKP) is one of the urgent threats to human health worldwide. The current study aims to evaluate the carbapenemase inhibitory potential of coumarin and to test its ability to restore meropenem activity against CRKP. Disk diffusion method was used to
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Transcriptomic meta-analysis to identify potential antifungal targets in Candida albicans BMC Microbiol. (IF 4.2) Pub Date : 2024-02-27 Zeinab Abdelmoghis Hefny, Boyang Ji, Ibrahim E. Elsemman, Jens Nielsen, Patrick Van Dijck
Candida albicans is a fungal pathogen causing human infections. Here we investigated differential gene expression patterns and functional enrichment in C. albicans strains grown under different conditions. A systematic GEO database search identified 239 “Candida albicans” datasets, of which 14 were selected after rigorous criteria application. Retrieval of raw sequencing data from the ENA database
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Escherichia coli and their potential transmission of carbapenem and colistin-resistant genes in camels BMC Microbiol. (IF 4.2) Pub Date : 2024-02-24 Marwa youseef, Fatma Karam, Mona Kadry, Mahmoud Elhariri, Rehab Elhelw
Camels harbouring multidrug-resistant Gram-negative bacteria are capable of transmitting various microorganisms to humans. This study aimed to determine the distribution of anti-microbial resistance among Escherichia coli (E. coli) isolated from the feces of apparently healthy camels in Egyptian abattoirs. Additionally, we sought to characterize Shiga toxin-producing E. coli (STEC) strains, assess
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Difference analysis and characteristics of incompatibility group plasmid replicons in gram-negative bacteria with different antimicrobial phenotypes in Henan, China BMC Microbiol. (IF 4.2) Pub Date : 2024-02-19 Ruyan Chen, Chenyu Li, Haoyu Ge, Jie Qiao, Lei Fang, Cailin Liu, Jianjun Gou, Xiaobing Guo
Multi-drug-resistant organisms (MDROs) in gram-negative bacteria have caused a global epidemic, especially the bacterial resistance to carbapenem agents. Plasmid is the common vehicle for carrying antimicrobial resistance genes (ARGs), and the transmission of plasmids is also one of the important reasons for the emergence of MDROs. Different incompatibility group plasmid replicons are highly correlated
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Development of a novel mycobiome diagnostic for fungal infection BMC Microbiol. (IF 4.2) Pub Date : 2024-02-19 Danielle Weaver, Lilyann Novak-Frazer, Maisie Palmer, Malcolm Richardson, Mike Bromley, Paul Bowyer
Amplicon-based mycobiome analysis has the potential to identify all fungal species within a sample and hence could provide a valuable diagnostic assay for use in clinical mycology settings. In the last decade, the mycobiome has been increasingly characterised by targeting the internal transcribed spacer (ITS) regions. Although ITS targets give broad coverage and high sensitivity, they fail to provide
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Effects of the alpine meadow in different phenological periods on rumen fermentation and gastrointestinal tract bacteria community in grazing yak on the Qinghai-Tibetan Plateau BMC Microbiol. (IF 4.2) Pub Date : 2024-02-19 Tongqing Guo, Xungang Wang, Qian Zhang, Yuna Jia, Yalin Wang, Lin Wei, Na Li, Xianli Xu, Hongjin Liu, Linyong Hu, Na Zhao, Shixiao Xu
In this study, we investigated the effects of alpine meadow in different phenological periods on ruminal fermentation, serum biochemical indices, and gastrointestinal tract microbes in grazing yak on the Qinghai-Tibetan Plateau. A total of eighteen female freely grazing yaks with an average age of 3 years old and a body weight of 130 ± 19 kg were selected. According to the plant phenological periods
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Emergence of multidrug-resistant Bacillus spp. derived from animal feed, food and human diarrhea in South-Eastern Bangladesh BMC Microbiol. (IF 4.2) Pub Date : 2024-02-19 Md Atiqul Haque, Huilong Hu, Jiaqi Liu, Md Aminul Islam, Foysal Hossen, Md Arifur Rahman, Firoz Ahmed, Cheng He
Antimicrobial resistance poses a huge risk to human health worldwide, while Bangladesh is confronting the most severe challenge between the food supply and the huge consumption of antibiotics annually. More importantly, probiotics containing Bacillus spp. are claimed to be an alternative to antimicrobial stewardship programs. However, their antibiotic resistance remains elusive. Thus, we employed the
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What happens to Bifidobacterium adolescentis and Bifidobacterium longum ssp. longum in an experimental environment with eukaryotic cells? BMC Microbiol. (IF 4.2) Pub Date : 2024-02-19 Dominika Jakubczyk, Katarzyna Leszczyńska, Katarzyna Pacyga-Prus, Dominika Kozakiewicz, Wioletta Kazana-Płuszka, Dominika Gełej, Paweł Migdał, Roksana Kruszakin, Agnieszka Zabłocka, Sabina Górska
The impact of probiotic strains on host health is widely known. The available studies on the interaction between bacteria and the host are focused on the changes induced by bacteria in the host mainly. The studies determining the changes that occurred in the bacteria cells are in the minority. Within this paper, we determined what happens to the selected Bifidobacterium adolescentis and Bifidobacterium
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Outbreak of colistin and carbapenem-resistant Klebsiella pneumoniae ST16 co-producing NDM-1 and OXA-48 isolates in an Iranian hospital BMC Microbiol. (IF 4.2) Pub Date : 2024-02-17 Rahimeh Sanikhani, Mojtaba Akbari, Majid Hosseinzadeh, Mansour Siavash, Farzad Badmasti, Hamid Solgi
Colistin and carbapenem-resistant Klebsiella pneumoniae (Col-CRKP) represent a significant and constantly growing threat to global public health. We report here an outbreak of Col-CRKP infections during the fifth wave of COVID-19 pandemic. The outbreak occurred in an intensive care unit with 22 beds at a teaching university hospital, Isfahan, Iran. We collected eight Col-CRKP strains from seven patients
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Optimized bacterial community characterization through full-length 16S rRNA gene sequencing utilizing MinION nanopore technology BMC Microbiol. (IF 4.2) Pub Date : 2024-02-16 Alessandro Bertolo, Ezra Valido, Jivko Stoyanov
Accurate identification of bacterial communities is crucial for research applications, diagnostics, and clinical interventions. Although 16S ribosomal RNA (rRNA) gene sequencing is a widely employed technique for bacterial taxonomic classification, it often results in misclassified or unclassified bacterial taxa. This study sought to refine the full-length 16S rRNA gene sequencing protocol using the
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Metagenomic analysis of gut microbiome illuminates the mechanisms and evolution of lignocellulose degradation in mangrove herbivorous crabs BMC Microbiol. (IF 4.2) Pub Date : 2024-02-13 Tom Kwok Lun Hui, Irene Ching Nam Lo, Karen Ka Wing Wong, Chandler Tsz To Tsang, Ling Ming Tsang
Sesarmid crabs dominate mangrove habitats as the major primary consumers, which facilitates the trophic link and nutrient recycling in the ecosystem. Therefore, the adaptations and mechanisms of sesarmid crabs to herbivory are not only crucial to terrestrialization and its evolutionary success, but also to the healthy functioning of mangrove ecosystems. Although endogenous cellulase expressions were
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ST218 Klebsiella pneumoniae became a high-risk clone for multidrug resistance and hypervirulence BMC Microbiol. (IF 4.2) Pub Date : 2024-02-12 Ping Yang, Chao Liu, Pengcheng Du, Juan Yi, Zhenchao Wu, Jiajia Zheng, Ning Shen, Liyan Cui, Ming Lu
The occurrence of multidrug-resistant and hypervirulent Klebsiella pneumoniae (MDR-hvKp) worldwide poses a great challenge for public health. Few studies have focused on ST218 MDR-hvKp. Retrospective genomic surveillance was conducted at the Peking University Third Hospital from 2017 and clinical information was obtained. To understand genomic and microbiological characteristics, antimicrobial susceptibility
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Anti-Acinetobacter Baumannii single-chain variable fragments provide therapeutic efficacy in an immunocompromised mouse pneumonia model BMC Microbiol. (IF 4.2) Pub Date : 2024-02-10 Eilnaz Basardeh, Somayeh Piri-Gavgani, Hamid Reza Moradi, Masoumeh Azizi, Parastoo Mirzabeigi, Farzaneh Nazari, Mostafa Ghanei, Fereidoun Mahboudi, Fatemeh Rahimi-Jamnani
The emergence of carbapenem-resistant and extensively drug-resistant (XDR) Acinetobacter baumannii as well as inadequate effective antibiotics calls for an urgent effort to find new antibacterial agents. The therapeutic efficacy of two human scFvs, EB211 and EB279, showing growth inhibitory activity against A. baumannii in vitro, was investigated in immunocompromised mice with A. baumannii pneumonia
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The anti-staphylococcal fusidic acid as an efflux pump inhibitor combined with fluconazole against vaginal candidiasis in mouse model BMC Microbiol. (IF 4.2) Pub Date : 2024-02-10 Salwa E. Gomaa, Hisham A. Abbas, Fatma A. Mohamed, Mohamed A. M. Ali, Tarek M. Ibrahim, Alyaa S. Abdel Halim, Mashael A. Alghamdi, Basem Mansour, Anis Ahmad Chaudhary, Amr Elkelish, Fehmi Boufahja, Wael A. H. Hegazy, Fatma Al-zahraa A. Yehia
Candida albicans is the most common fungus that causes vaginal candidiasis in immunocompetent women and catastrophic infections in immunocompromised patients. The treatment of such infections is hindered due to the increasing emergence of resistance to azoles in C. albicans. New treatment approaches are needed to combat candidiasis especially in the dwindled supply of new effective and safe antifungals
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Bacterial community structure and co-occurrence networks in the rhizosphere and root endosphere of the grafted apple BMC Microbiol. (IF 4.2) Pub Date : 2024-02-10 Hui Cao, Longxiao Xu, Jianfei Song, Mi Xun, Weiwei Zhang, Hongqiang Yang
Compared with aerial plant tissues (such as leaf, stem, and flower), root-associated microbiomes play an indisputable role in promoting plant health and productivity. We thus explored the similarities and differences between rhizosphere and root endosphere bacterial community in the grafted apple system. Using pot experiments, three microhabitats (bulk soil, rhizosphere and root endosphere) samples
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Regulation of overexpressed efflux pump encoding genes by cinnamon oil and trimethoprim to abolish carbapenem-resistant Acinetobacter baumannii clinical strains BMC Microbiol. (IF 4.2) Pub Date : 2024-02-08 Neveen M. Saleh, Hadeer Ezzat, Gharieb S. El-Sayyad, Hamdallah Zedan
Resistance mechanisms are a shelter for Acinetobacter baumannii to adapt to our environment which causes difficulty for the infections to be treated and WHO declares this organism on the top of pathogens priority for new drug development. The most common mechanism that develops drug resistance is the overexpression of the efflux pump, especially Resistance-nodulation-cell division (RND) family, to
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Development of a multiple cross displacement amplification combined with nanoparticles-based biosensor assay for rapid and sensitive detection of Streptococcus pyogenes BMC Microbiol. (IF 4.2) Pub Date : 2024-02-07 Zhiqian Dou, Ling Xie, Meiling Gao, Dexi Liu
S. pyogenes, is a primary pathogen that leads to pharyngitis and can also trigger severe conditions like necrotizing fasciitis and streptococcal toxic shock syndrome (STSS), often resulting in high mortality rates. Therefore, prompt identification and appropriate treatment of S. pyogenes infections are crucial in preventing the worsening of symptoms and alleviating the disease's impact. In this study
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Meat and meat products as potential sources of emerging MDR Bacillus cereus: groEL gene sequencing, toxigenic and antimicrobial resistance BMC Microbiol. (IF 4.2) Pub Date : 2024-02-07 Abdelazeem M. Algammal, Hamza M. Eid, Saad Alghamdi, Hanaa Ghabban, Roba Alatawy, Enas A. Almanzalawi, Tahani M. Alqahtani, Sabreen G. Elfouly, Gihan M. Mohammed, Helal F. Hetta, Reham M. El-Tarabili
Bacillus cereus is implicated in severe foodborne infection in humans. This study intended to assess the occurrence, groEL gene sequencing, biofilm production, and resistance profiles of emerged multidrug resistant (MDR) B. cereus in meat and meat product samples. Moreover, this work highlights the virulence and toxigenic genes (hblABCD complex, nheABC complex, cytK, ces, and pc-plc) and antimicrobial
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Mapping pathogenic bacteria resistance against common antibiotics and their potential susceptibility to methylated white kidney bean protein BMC Microbiol. (IF 4.2) Pub Date : 2024-02-05 Mahmoud Sitohy, Gamal Enan, Seham Abdel-Shafi, Neveen Abou El-Wafa, Nashwa El-Gazzar, Ali Osman, Basel Sitohy
As antibiotics cannot inhibit multidrug-resistant bacteria (MDR), continuous research is mandatory to find other antibacterials from natural resources. Native legume proteins and their modified forms exhibited broad spectra of high antimicrobial activities. Sixteen bacterial isolates were mapped for antibiotic resistance, showing resistance in the range of (58–92%) and (42–92%) in the case of the Gram-negative
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Chronic intestinal pseudo-obstruction: associations with gut microbiota and genes expression of intestinal serotonergic pathway BMC Microbiol. (IF 4.2) Pub Date : 2024-02-02 Giulia Radocchia, Massimiliano Marazzato, Karim Ben Harbi, Elena Capuzzo, Fabrizio Pantanella, Roberto De Giorgio, Matteo Guarino, Anna Costanzini, Letizia Zenzeri, Pasquale Parisi, Alessandro Ferretti, Enrico Felici, Anna Teresa Palamara, Giovanni Di Nardo, Serena Schippa
Pediatric chronic intestinal pseudo-obstruction (PIPO) is a rare disease characterized by symptoms and radiological signs suggestive of intestinal obstruction, in the absence of lumen-occluding lesions. It results from an extremely severe impairment of propulsive motility. The intestinal endocrine system (IES) jointly with the enteric nervous system (ENS) regulates secreto-motor functions via different
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Draft genome sequence of novel Candidatus Ornithobacterium hominis carrying antimicrobial resistance genes in Egypt BMC Microbiol. (IF 4.2) Pub Date : 2024-02-02 Nada Ahmed, Marwa Azab, Shymaa Enany, Amro Hanora
Candidatus Ornithobacterium hominis (O. hominis), which was identified in nasopharyngeal swabs from Egypt, has been associated with respiratory disorders in humans. O. hominis, a recently identified member of the Flavobacteriaceae family, belongs to the largest family within the Bacteroidetes phylum. This family includes hundreds of species and 90 genera, including major human pathogens such as Capnocytophaga
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Identification of Campylobacter jejuni and Campylobacter coli genes contributing to oxidative stress response using TraDIS analysis BMC Microbiol. (IF 4.2) Pub Date : 2024-02-01 Emily Stoakes, Xuanlin Chen, Lajos Kalmar, Dave Baker, Rhiannon Evans, Steven Rudder, Andrew J. Grant
Campylobacter jejuni and Campylobacter coli are the major causative agents of bacterial gastroenteritis worldwide and are known obligate microaerophiles. Despite being sensitive to oxygen and its reduction products, both species are readily isolated from animal food products kept under atmospheric conditions where they face high oxygen tension levels. In this study, Transposon Directed Insertion-site
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Gut microbiota composition and changes in patients with sepsis: potential markers for predicting survival BMC Microbiol. (IF 4.2) Pub Date : 2024-02-01 Feiyu Luan, Yang Zhou, Xiaohui Ma, Yue Li, Yahui Peng, Xiaonan Jia, Nana Li, Xibo Wang, Yinghao Luo, Mingyin Man, Qianqian Zhang, Chunying Wang, Kaijiang Yu, Mingyan Zhao, Changsong Wang
Sepsis can cause immune dysregulation and multiple organ failure in patients and eventually lead to death. The gut microbiota has demonstrated its precise therapeutic potential in the treatment of various diseases. This study aimed to discuss the structural changes of the gut microbiota in patients with sepsis and to analyze the differences in the gut microbiota of patients with different prognoses
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Purification and characterization of L-arginine deiminase from Penicillium chrysogenum BMC Microbiol. (IF 4.2) Pub Date : 2024-01-31 Hamed M. El-Shora, Nessma A. El-Zawawy, Mohamed A. Abd El-Rheem, Metwally A. Metwally
L-arginine deiminase (ADI, EC 3.5.3.6) hydrolyzes arginine to ammonia and citrulline which is a natural supplement in health care. ADI was purified from Penicillium chrysogenum using 85% ammonium sulfate, DEAE-cellulose and Sephadex G200. ADI was purified 17.2-fold and 4.6% yield with a specific activity of 50 Umg− 1 protein. The molecular weight was 49 kDa. ADI expressed maximum activity at 40oC and
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Bioprocessing of Epothilone B from Aspergillus fumigatus under solid state fermentation: Antiproliferative activity, tubulin polymerization and cell cycle analysis BMC Microbiol. (IF 4.2) Pub Date : 2024-01-30 Ashraf S. A. El-Sayed, Ahmed Shindia, Hala Ammar, Mohamed G. Seadawy, Samar A. Khashana
Epothilone derivatives have been recognized as one of the most powerful anticancer drugs towards solid tumors, for their unique affinity to bind with β-tubulin microtubule arrays, stabilizing their disassembly, causing cell death. Sornagium cellulosum is the main source for Epothilone, however, the fermentation bioprocessing of this myxobacteria is the main challenge for commercial production of Epothilone
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Atopic dermatitis pediatric patients show high rates of nasal and intestinal colonization by methicillin-resistant Staphylococcus aureus and coagulase-negative staphylococci BMC Microbiol. (IF 4.2) Pub Date : 2024-01-29 Mariana Fernandes Augusto de Oliveira, Daiane Bitencourt Agne, Ludmila Sento Sé Bastos, Laura Maria Andrade de Oliveira, Simone Saintive, Ekaterini Simoes Goudouris, Evandro Alves do Prado, Henrique Fragoso dos Santos, Raphael da Silva Pereira, Fernanda Sampaio Cavalcante, Dennis de Carvalho Ferreira, Kátia Regina Netto dos Santos
Atopic dermatitis (AD) patients have high rates of colonization by Staphylococcus aureus, which has been associated with worsening of the disease. This study characterized Staphylococcus spp isolates recovered from nares and feces of pediatric patients with AD in relation to antimicrobial susceptibility, staphylococcal cassette chromosome mec (SCCmec) type, presence of pvl genes and clonality. Besides
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Potential public health hazards related to consumption of poultry contaminated with antibiotic resistant Listeria monocytogenes in Egypt BMC Microbiol. (IF 4.2) Pub Date : 2024-01-29 Amira Ibrahim Zakaria, Rana Fahmi Sabala
Listeria monocytogenes is an important foodborne pathogen that incorporated into many serious infections in human especially immunocompromised individuals, pregnant women, the elderly, and newborns. The consumption of food contaminated with such bacteria is considered a source of potential risk for consumers. Therefore, a total of 250 poultry purchased in highly popular poultry stores besides 50 swabs
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The mysterious anelloviruses: investigating its role in human diseases BMC Microbiol. (IF 4.2) Pub Date : 2024-01-29 Mohammad Sabbaghian, Hamidreza Gheitasi, Ali Akbar Shekarchi, Ahmad Tavakoli, Vahdat Poortahmasebi
Anelloviruses (AVs) that infect the human population are members of the Anelloviridae family. They are widely distributed in human populations worldwide. Torque teno virus (TTV) was the first virus of this family to be identified and is estimated to be found in the serum of 80–90% of the human population. Sometime after the identification of TTV, Torque teno mini virus (TTMV) and Torque teno midi virus
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Bioflocculation of pollutants in wastewater using flocculant derived from Providencia huaxiensis OR794369.1 BMC Microbiol. (IF 4.2) Pub Date : 2024-01-29 Tlou Nelson Selepe, Tsolanku Sidney Maliehe
Water pollution has become a major environmental and health concern due to increasing population and industrialisation. Microbial flocculants are promising agents for treatment of contaminated water owing to their effectiveness, eco-friendliness, and high biosafety levels. In this study, culture conditions of Providencia huaxiensis OR794369.1 were optimised and its bioflocculant was extracted, characterised
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Identification and pathogenicity of Fusarium spp. associated with tea wilt in Zhejiang Province, China BMC Microbiol. (IF 4.2) Pub Date : 2024-01-27 Zhaoyang Tang, Jing Zhu, Qiujin Song, Paul Daly, Liya Kong, Luqian He, Agen Li, Jun Lou, Zhanqi Wang, Liqin Zhang, Lijing Min
Tea is one of the most widely consumed beverages in the world, with significant economic and cultural value. However, tea production faces many challenges due to various biotic and abiotic stresses, among which fungal diseases are particularly devastating. To understand the identity and pathogenicity of isolates recovered from tea plants with symptoms of wilt, phylogenetic analyses and pathogenicity
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Evaluation of a novel lysis-based sample processing method to optimize Vibrio vulnificus detecting by loop-mediated isothermal amplification assay BMC Microbiol. (IF 4.2) Pub Date : 2024-01-27 Lei Zhang, Jianfei Liu, Kewei Qin, Chenglin Wu, Hui Ma, Lijun Zhou
Vibrio vulnificus exists as one of the most serious foodborne pathogens for humans, and rapid and sensitive detection methods are needed to control its infections. As an emerging method, The Loop-Mediated Isothermal Amplification (LAMP) assay has been applied to the early detection of various foodborne pathogens due to its high efficiency, but sample preprocessing still prolongs the complete detection
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Prevalence of S. aureus and/or MRSA in hospitalized patients with diabetic foot and establishment of LAMP methods for rapid detection of the SCCmec gene BMC Microbiol. (IF 4.2) Pub Date : 2024-01-26 Chunxia Qi, Xiangrong Luo, Jiali Huang, Danli Kong, Yali Zhang, Mengchen Zou, Hao Zhou
Patients with diabetic feet are prone to be infected due to the impaired immune system. However, the prognostic outcome of different microbial infections remains controversial. Identification and rapid screening of the pathogenic microorganisms that pose the greatest threat to the prognosis of patients with diabetic foot infections (DFIs) is critical. Clinical data were statistically analyzed, which
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High frequency of antimicrobial resistance in Salmonella and Escherichia coli causing diarrheal diseases at the Yirimadio community health facility, Mali BMC Microbiol. (IF 4.2) Pub Date : 2024-01-23 Bintou Diarra, Ibréhima Guindo, Boī Koné, Maīmouna Dembélé, Ibrahim Cissé, Souleymane Thiam, Kadidia Konaté, Mamadou Tékété, Almoustapha Maīga, Oumou Maīga, Lassina Timbiné, Abdoulaye Djimde
Diarrhoea is a public health problem, especially in developing countries where it is the second leading cause of child mortality. In Low Income Countries like in Mali, self-medication and inappropriate use of antibiotics due to the scarcity of complementary diagnostic systems can lead to the development of multidrug-resistant bacteria causing diarrhoea. The objective of this work was to determine the
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Sex, health status and habitat alter the community composition and assembly processes of symbiotic bacteria in captive frogs BMC Microbiol. (IF 4.2) Pub Date : 2024-01-23 Senlin Liu, Sewar Imad, Sarfraz Hussain, Shuiqing Xiao, Xiaowei Yu, Hui Cao
Frogs are critical economic animals essential to agricultural ecosystem equilibrium. However, Meningitis-like Infectious Disease (MID) often affects them in agricultural settings. While frog-associated microbiota contribute to elemental cycling and immunity, the effects of frog sex and health on gut bacteria remain understudied, and the relationship between frog habitat and soil microbes is unclear
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Genetic approach toward linkage of Iran 2012–2016 cholera outbreaks with 7th pandemic Vibrio cholerae BMC Microbiol. (IF 4.2) Pub Date : 2024-01-22 Fatemeh Jalalizadeh, Elisabeth Njamkepo, François-Xavier Weill, Forough Goodarzi, Marjan Rahnamaye-Farzami, Roghieh Sabourian, Bita Bakhshi
Vibrio cholerae, as a natural inhabitant of the marine environment is among the world-leading causes of diarrheal diseases. The present study aimed to investigate the genetic relatedness of Iran 2012–2016 V. cholerae outbreaks with 7th pandemic cholera and to further characterize the non-ST69/non-ST75 sequence types strains by whole-genome sequencing (WGS). Twenty V. cholerae isolates related to 2012