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ompA Sequencing and Multilocus Sequence Typing of Lymphogranuloma Venereum Cases in Buenos Aires Reveal New Chlamydia trachomatis Genotypes Microorganisms (IF 4.5) Pub Date : 2024-03-15 Karina Andrea Büttner, Andrea Carolina Entrocassi, María Lucía Gallo Vaulet, Deysi López Aquino, Dolores Caffarena, Luciana La Rosa, Laura Svidler López, Osvaldo Degregorio, Björn Herrmann, Marcelo Rodríguez Fermepin
Since the Lymphogranuloma venereum (LGV) outbreak was first described in Buenos Aires in 2017, the detected strains presented peculiar characteristics. Our goal was to increase the understanding of the strains involved in the LGV outbreak in Argentina. We characterized the ompA gene sequences, using Sanger sequencing, of 88 LGV strains from 239 symptomatic patients in Buenos Aires enrolled between
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Therapy of Mycobacterium abscessus Infections in Solid Organ Transplant Patients Microorganisms (IF 4.5) Pub Date : 2024-03-16 Lubna Osman, Christopher Lopez, Yoichiro Natori, Shweta Anjan, Julia Bini Viotti, Jacques Simkins
Mycobacterium abscessus complex (MABC), a rapidly growing Mycobacterium, is one of the most common causes of non-tuberculous mycobacteria (NTM) infections in the United States of America, and it has been associated with a wide spectrum of infections in immunocompetent and immunosuppressed individuals. Eradicating MABC is very challenging, even with prolonged combination therapies. The management of
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RNA Interference Therapeutics for Chronic Hepatitis B: Progress, Challenges, and Future Prospects Microorganisms (IF 4.5) Pub Date : 2024-03-17 Laura Sneller, Christine Lin, Angie Price, Shyam Kottilil, Joel V. Chua
Chronic hepatitis B (CHB) is a global health challenge that can result in significant liver-related morbidity and mortality. Despite a prophylactic vaccine being available, patients already living with CHB often must engage in lifelong therapy with nucleoside analogues. However, the potential of RNA interference (RNAi) therapeutics as a promising avenue for CHB treatment is being explored. RNAi, particularly
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A Mini-Review on the Common Antiviral Drug Targets of Coronavirus Microorganisms (IF 4.5) Pub Date : 2024-03-17 Jun Wang, Qinghe Zhu, Xiaoxu Xing, Dongbo Sun
Coronaviruses in general are a zoonotic pathogen with significant cross-species transmission. They are widely distributed in nature and have recently become a major threat to global public health. Vaccines are the preferred strategy for the prevention of coronaviruses. However, the rapid rate of virus mutation, large number of prevalent strains, and lag in vaccine development contribute to the continuing
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Effects of Six Natural Compounds and Their Derivatives on the Control of Coccidiosis in Chickens Microorganisms (IF 4.5) Pub Date : 2024-03-17 Yue Hou, Bo Han, Zehua Lin, Qizheng Liu, Zhenhe Liu, Hongbin Si, Dandan Hu
Chicken coccidiosis costs the poultry industry over GBP 10 billion per year. The main method of preventing and controlling coccidiosis in chickens continues to be the use of drugs. Unfortunately, the prevalence of drug resistance in the field reduces or even eliminates the effectiveness of drugs, and drug residues in the food supply chain can also can be harmful to humans. Therefore, safe and effective
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Transcriptomics of Besnoitia besnoiti-Infected Fibroblasts Reveals Hallmarks of Early Fibrosis and Cancer Progression Microorganisms (IF 4.5) Pub Date : 2024-03-15 María Fernández-Álvarez, Pilar Horcajo, Alejandro Jiménez-Meléndez, Pablo Angulo Lara, Ana Huertas-López, Francisco Huertas-López, Ignacio Ferre, Luis Miguel Ortega-Mora, Gema Álvarez-García
Endothelial injury, inflammatory infiltrate and fibrosis are the predominant lesions in the testis of bulls with besnoitiosis that may result in sterility. Moreover, fibroblasts, which are key players in fibrosis, are parasite target cells in a Besnoitia besnoiti chronic infection. This study aimed to decipher the molecular basis that underlies a drift toward fibrosis during the disease progression
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Investigation of Yellow Fever Virus at the Human–Animal Interface after a Zika Virus Outbreak in Midwest Brazil Microorganisms (IF 4.5) Pub Date : 2024-03-16 Lis Alvarez Baranowski, Helver Gonçalves Dias, Débora Familiar-Macedo, Gilberto Sabino-Santos, Heitor Miraglia Herrera, Renata Dezengrini Slhessarenko, Flávia Barreto dos Santos, Alex Pauvolid-Corrêa
Following the first report of zika virus in March 2015, Brazil experienced its largest sylvatic yellow fever outbreak between 2016 and 2019. This study aimed to investigate the circulation of yellow fever virus (YFV) in non-human primates (NHPs) and mosquitoes collected in urban parks and other metropolitan areas of midwest Brazil between 2017 and 2018. Whole blood samples from 80 NHPs, including 48
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Removal of Phenol by Rhodococcus opacus 1CP after Dormancy: Insight into Enzymes’ Induction, Specificity, and Cells Viability Microorganisms (IF 4.5) Pub Date : 2024-03-16 Natalia S. Egozarian, Elena V. Emelyanova, Nataliya E. Suzina, Olesya I. Sazonova, Valentina N. Polivtseva, Tatiana O. Anokhina, Yonghong Wu, Inna P. Solyanikova
Biodegradation of phenol is an effective method for removing this toxicant from contaminated sites. Phenol is a toxic compound for living cells, so many bacteria degrade phenol in relatively low concentrations, up to 0.75 g L−1. The Rhodococcus opacus strain 1CP is an effective destructor of a wide range of pollutants. In the absence of a carbon source in the medium, cells of the R. opacus 1CP strain
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Physicochemical Properties and Microbiome of Vineyard Soils from DOP Ribeiro (NW Spain) Are Influenced by Agricultural Management Microorganisms (IF 4.5) Pub Date : 2024-03-16 Pilar Blanco, Isaac Rodríguez, Victoria Fernández-Fernández, María Ramil, David Castrillo, Marta Acín-Albiac, Irene Adamo, Clara Fernández-Trujillo, Beatriz García-Jiménez, Alberto Acedo, Noemi Calvo-Portela, Andrea Parente-Sendín, Lara Acemel-Míguez, Flora Alonso-Vega
Agricultural management influences the soil ecosystem by affecting its physicochemical properties, residues of pesticides and microbiome. As vineyards grow crops with the highest incidence of pesticides, the aim of this study was to evaluate the impact of conventional and sustainable management systems of vineyards from DOP Ribeiro on the soil’s condition. Samples from soils under three different management
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Identification and Pathogenicity of Fusarium Fungi Associated with Dry Rot of Potato Tubers Microorganisms (IF 4.5) Pub Date : 2024-03-16 Olga Gavrilova, Aleksandra Orina, Ilya Trubin, Tatiana Gagkaeva
Dry rot of potato tubers is a harmful disease caused by species of the Fusarium genus. Studies on the composition and features of Fusarium spp. that cause the disease in Russia are limited. Thirty-one Fusarium strains belonging to the F. sambucinum species complex (FSAMSC) and F. solani species complex (FSSC) were accurately identified using multilocus phylogenetic analysis of the tef and rpb2 loci
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Characterization and Implications of IncP-2A Plasmid pMAS152 Harboring Multidrug Resistance Genes in Extensively Drug-Resistant Pseudomonas aeruginosa Microorganisms (IF 4.5) Pub Date : 2024-03-12 Li Mei, Yang Song, Xiao Liu, Kun Li, Xu Guo, Li Liu, Yang Liu, Zisis Kozlakidis, Io Hong Cheong, Duochun Wang, Qiang Wei
Bacterial antimicrobial resistance (AMR) poses a significant global public health challenge. The escalation of AMR is primarily attributed to the horizontal gene transfer (HGT) of antibiotic resistance genes (ARGs), often facilitated by plasmids. This underscores the critical need for a comprehensive understanding of the resistance mechanisms and transmission dynamics of these plasmids. In this study
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Nitrogen Application and Rhizosphere Effect Exert Opposite Effects on Key Straw-Decomposing Microorganisms in Straw-Amended Soil Microorganisms (IF 4.5) Pub Date : 2024-03-13 Yuanzheng Zhao, Shiyu Wang, Meiling Zhang, Li Zeng, Liyu Zhang, Shuyu Huang, Rong Zhang, Wei Zhou, Chao Ai
Crop residue decomposition is an important part of the carbon cycle in agricultural ecosystems, and microorganisms are widely recognized as key drivers during this process. However, we still know little about how nitrogen (N) input and rhizosphere effects from the next planting season impact key straw-decomposing microbial communities. Here, we combined amplicon sequencing and DNA-Stable Isotope Probing
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Gut Microbiome and Disorders of the Gastrointestinal Tract Microorganisms (IF 4.5) Pub Date : 2024-03-13 Alexander Hammerhøj, John Mark Gubatan, Ole Haagen Nielsen
The protective intestinal epithelial barrier is constantly exposed to more than 100 trillion commensal microorganisms (bacteria, archaea, viruses, and fungi), i [...]
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Spatiotemporal Dynamics of Assyrtiko Grape Microbiota Microorganisms (IF 4.5) Pub Date : 2024-03-14 Konstantinos Tegopoulos, Theodora Tsirka, Christos Stekas, Eleni Gerasimidi, George Skavdis, Petros Kolovos, Maria E. Grigoriou
Vitis vinifera, an economically significant grapevine species, is known for wine, juice, and table grape production. The berries of wine grapes host a diverse range of microorganisms influencing both grapevine health and the winemaking process. Indigenous to Greece, the emblematic variety Assyrtiko, renowned for high-quality white wines, originated from Santorini and spread to various Greek regions
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Acanthamoeba spp. as Factors for Severe Infectious Diseases in Humans Microorganisms (IF 4.5) Pub Date : 2024-03-14 Lidia Chomicz, Jacek P. Szaflik, Wanda Baltaza
Acanthamoeba spp [...]
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Food Security and Foodborne Mycotoxicoses—What Should Be the Adequate Risk Assessment and Regulation? Microorganisms (IF 4.5) Pub Date : 2024-03-14 Stoycho D. Stoev
The purpose of this review is to elucidate the actual threat of the most prevalent mycotoxins in agricultural commodities and human/animal food/feed for the induction of foodborne diseases or ailments. The underestimated hazard of combined mycotoxin uptake by animals or humans is critically discussed with regard to synergistic or additive interaction between some target mycotoxins. The real toxicity
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Antifungal Activity of Phyllospheric Bacteria Isolated from Coffea arabica against Hemileia vastatrix Microorganisms (IF 4.5) Pub Date : 2024-03-14 Katty Ogata-Gutiérrez, Carolina Chumpitaz-Segovia, Jesus Lirio-Paredes, Doris Zúñiga-Dávila
Peru is one of the leading countries that produce and export specialty coffees, favorably positioned in the international markets for its physical and organoleptic cup qualities. In recent years, yellow coffee rust caused by the phytopathogenic fungus Hemileia vastatrix stands out as one of the main phytosanitary diseases that affect coffee culture yields. Many studies have demonstrated bacteria antagonistic
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Exploration of the Vermiculite-Induced Bacterial Community and Co-Network Successions during Sludge–Waste Mushroom Co-Composting Microorganisms (IF 4.5) Pub Date : 2024-03-15 Zhaojing Yu, Bin Wang, Xiaoyan Wu, Runlan Yu, Li Shen, Xueling Wu, Jiaokun Li, Yuandong Liu, Weimin Zeng
Vermiculite is a clay mineral with unique physical properties that plays a significant role in plant cultivation, soil remediation, and solid waste management. In this research, we first explored how vermiculite-to-microbe interactions evolved during sludge–waste mushroom residue co-composting. Vermiculite’s addition had a substantial impact on the microbial α and β diversities, significantly changed
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Identification and Characterization of the Gene Responsible for the O3 Mating Type Substance in Paramecium caudatum Microorganisms (IF 4.5) Pub Date : 2024-03-15 Yuta Chiba, Yasuhiro Takenaka, Nobuyuki Haga
The process of sexual reproduction in eukaryotes starts when gametes from two different sexes encounter each other. Paramecium, a unicellular eukaryote, undergoes conjugation and uses a gametic nucleus to enter the sexual reproductive process. The molecules responsible for recognizing mating partners, hypothetically called mating-type substances, are still unclear. We have identified an O3-type mating
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Prevalence and Characteristics of Plasmid-Encoded Serine Protease EspP in Clinical Shiga Toxin-Producing Escherichia coli Strains from Patients in Sweden Microorganisms (IF 4.5) Pub Date : 2024-03-15 Lei Wang, Ying Hua, Xiangning Bai, Ji Zhang, Sara Mernelius, Milan Chromek, Anne Frykman, Sverker Hansson, Andreas Matussek
Shiga toxin-producing Escherichia coli (STEC) infection can cause a broad spectrum of symptoms spanning from asymptomatic shedding to mild and bloody diarrhea (BD) and even life-threatening hemolytic-uremic syndrome (HUS). As a member of the serine protease autotransporters of Enterobacteriaceae (SPATE) family, EspP has the ability to degrade human coagulation factor V, leading to mucosal bleeding
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Characterize the Growth and Metabolism of Acidithiobacillus ferrooxidans under Electroautotrophic and Chemoautotrophic Conditions Microorganisms (IF 4.5) Pub Date : 2024-03-15 Quansheng Wang, Haijun Long, Huiqi Wang, Maggie C. Y. Lau Vetter
Acidophiles are capable of surviving in extreme environments with low pH. Acidithiobacillus ferrooxidans is a typical acidophilic bacterium that has been extensively studied when grown chemoautotrophically, i.e. when it derives energy from oxidation of Fe2+ or reduced inorganic sulfur compounds (RISCs). Although it is also known to grow with electrons supplied by solid electrodes serving as the sole
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Impact of Swabbing Location, Self-Swabbing, and Food Intake on SARS-CoV-2 RNA Detection Microorganisms (IF 4.5) Pub Date : 2024-03-15 Sarah Dräger, Flavio Bruni, Melina Bernasconi, Anya Hammann-Hänni, Vlastimil Jirasko, Alexander Tanno, Yves Blickenstorfer, Karoline Leuzinger, Hans H. Hirsch, Michael Osthoff
This study compared SARS-CoV-2 RNA loads at different anatomical sites, and the impact of self-swabbing and food intake. Adult symptomatic patients with SARS-CoV-2 or non-SARS-CoV-2 respiratory tract infection were included between 2021 and 2022. Patients performed a nasal and buccal swab before a professionally collected nasopharyngeal/oropharyngeal swab (NOPS). Buccal swabs were collected fasting
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Enhancing Benzo[a]pyrene Degradation by Pantoea dispersa MSC14 through Biostimulation with Sodium Gluconate: Insights into Mechanisms and Molecular Regulation Microorganisms (IF 4.5) Pub Date : 2024-03-15 La Lai, Shuqi Li, Shaoping Zhang, Manchun Liu, Lianwei Xia, Yuan Ren, Tangbing Cui
We investigated biostimulation as an effective strategy for enhancing the degradation efficiency of recalcitrant organic compounds, with MSC14 (a novel polycyclic aromatic hydrocarbon degrading bacterium Pantoea dispersa MSC14) as the study material. Here, we investigated the impact of sodium gluconate on MSC14-mediated degradation of B[a]p. This study focused on the application of sodium gluconate
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Viroid Replication, Movement, and the Host Factors Involved Microorganisms (IF 4.5) Pub Date : 2024-03-12 Yuhong Zhang, Yuxin Nie, Luyou Wang, Jian Wu
Viroids represent distinctive infectious agents composed solely of short, single-stranded, circular RNA molecules. In contrast to viruses, viroids do not encode for proteins and lack a protective coat protein. Despite their apparent simplicity, viroids have the capacity to induce diseases in plants. Currently, extensive research is being conducted on the replication cycle of viroids within both the
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Microbial Symphony: Navigating the Intricacies of the Human Oral Microbiome and Its Impact on Health Microorganisms (IF 4.5) Pub Date : 2024-03-13 Rahul Bhandary, Geethu Venugopalan, Amitha Ramesh, Guilia Margherita Tartaglia, Ishita Singhal, Shahnawaz Khijmatgar
This comprehensive review delves into the forefront of research on the human oral microbiome, exploring recent advancements that span microbial colonization, state-of-the-art detection methodologies, and the complex interplay involved in disease progression. Through an exhaustive analysis of the contemporary literature, we illuminate the dynamic orchestration of microbial communities within the oral
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Yeast-Based Screening of Anti-Viral Molecules Microorganisms (IF 4.5) Pub Date : 2024-03-14 Vartika Srivastava, Ravinder Kumar, Aijaz Ahmad
Viruses are minuscule infectious agents that reproduce exclusively within the living cells of an organism and are present in almost every ecosystem. Their continuous interaction with humans poses a significant threat to the survival and well-being of everyone. Apart from the common cold or seasonal influenza, viruses are also responsible for several important diseases such as polio, rabies, smallpox
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A Novel Regulator PepR Regulates the Expression of Dipeptidase Gene pepV in Bacillus thuringiensis Microorganisms (IF 4.5) Pub Date : 2024-03-14 Xin Zhang, Hengjie Wang, Tinglu Yan, Yuhan Chen, Qi Peng, Fuping Song
Bacillus thuringiensis produces insecticidal crystal proteins encoded by cry or cyt genes and targets a variety of insect pests. We previously found that a strong promoter of a DeoR family transcriptional regulator (HD73_5014) can efficiently drive cry1Ac expression in B. thuringiensis HD73. Here, we investigated the regulation of neighbor genes by HD73_5014. The HD73_5014 homologs are widely distributed
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Exploring the Impact of Coconut Peat and Vermiculite on the Rhizosphere Microbiome of Pre-Basic Seed Potatoes under Soilless Cultivation Conditions Microorganisms (IF 4.5) Pub Date : 2024-03-14 Kan Yan, Yanni Ma, Songming Bao, Wandi Li, Yunjiao Wang, Chao Sun, Xin Lu, Juan Ran
Soilless cultivation of potatoes often utilizes organic coconut peat and inorganic vermiculite as growing substrates. The unique microbial communities and physicochemical characteristics inherent to each substrate significantly influence the microecological environment crucial for potato growth and breeding. This study analyzed environmental factors within each substrate and employed Illumina sequencing
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The Renin–Angiotensin System (RAS) in COVID-19 Disease: Where We Are 3 Years after the Beginning of the Pandemic Microorganisms (IF 4.5) Pub Date : 2024-03-14 Marco Prato, Natalia Tiberti, Cristina Mazzi, Federico Gobbi, Chiara Piubelli, Silvia Stefania Longoni
The RAS is a hormonal system playing a pivotal role in the control of blood pressure and electrolyte homeostasis, the alteration of which is associated with different pathologies, including acute respiratory distress syndrome (ARDS). As such, it is not surprising that a number of studies have attempted to elucidate the role and balance of the renin–angiotensin system (RAS) in COVID-19. In this review
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Advances in the Effects of Dietary Macronutrients on the Gut Microbiota of Tilapia (Oreochromis spp.) Microorganisms (IF 4.5) Pub Date : 2024-03-08 Weihao Ou, Zihe Guo, Ying Pan, Kai Huang, Yanqun Ma, Zhibiao Qin
The homeostasis of the intestinal microbiota of fish is beneficial to fish health, while food can affect the intestinal microbiota. Tilapia (Oreochromis spp.) has great economic value and is a good model to use in studying the digestion and absorption of nutrients. Furthermore, at present, due to a high demand and high price of high-quality feed raw materials, the nutritional composition of aquafeeds
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The Kocher–Caird Criteria for Pediatric Septic Arthritis of the Hip: Time for a Change in the Kingella Era? Microorganisms (IF 4.5) Pub Date : 2024-03-10 Silvia Valisena, Giacomo De Marco, Oscar Vazquez, Blaise Cochard, Christina Steiger, Romain Dayer, Dimitri Ceroni
Pediatric septic arthritis of the hip (SAH) in children is a severe pathology, requiring prompt diagnosis and treatment to avoid destructive sequelae of the joint. Its diagnosis can be challenging, however, due to its spectrum of manifestations and differential diagnosis. Last century, multiple research teams studied the curves of systemic inflammation markers to aid the differential diagnosis. Kocher
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Contamination and Control of Mycotoxins in Grain and Oil Crops Microorganisms (IF 4.5) Pub Date : 2024-03-12 Chenchen Zhang, Zheng Qu, Jie Hou, Yanpo Yao
Mycotoxins are carcinogenic, teratogenic and mutagenic toxic compounds produced by some filamentous fungi, which are extremely harmful to corn, rice, wheat, peanut, soybean, rapeseed and other grain and oil crops, and seriously threaten environmental safety, food safety and human health. With the rapid increase in the global population and the expansion of the main crop planting area, mycotoxin contamination
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An Exploratory Review of the Potential of Lytic Proteins and Bacteriophages for the Treatment of Tuberculosis Microorganisms (IF 4.5) Pub Date : 2024-03-12 Sibongile Mtimka, Priyen Pillay, Lusisizwe Kwezi, Ofentse Jacob Pooe, Tsepo Lebiletsa Tsekoa
Tuberculosis (TB) is a highly prevalent infectious disease that causes more than 1.5 million deaths a year. More than 25% of TB deaths occur in Africa, and TB is South Africa’s leading cause of death, with about 89,000 people dying of it yearly. The emergence of multidrug-resistant TB (MDR-TB) poses a significant threat to health security and could reverse the positive gains already made in the fight
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A Novel Transposon Tn7709 Harbors Multidrug Resistance Genes in a Pathogenic Aeromonas media Strain QST31 Microorganisms (IF 4.5) Pub Date : 2024-03-13 Baodi Shang, Xiaoyi Li, Xiaoping Zhang, Meiyan Zhang, Jie Kong, Jinle Wang, Aiping Tan, Feng Zhao, Defeng Zhang
Pathogenic Aeromonas spp. are the etiological agents of Motile Aeromonas Septicemia (MAS). This study aimed to identify the pathogen of diseased tadpoles (Quasipaa spinosa) and the antibiotic-resistance characteristics of this bacterium. A Gram-negative bacterium, named strain QST31, was isolated from the ascites of diseased tadpoles and was identified as Aeromonas media based on physiological and
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Indirect Effects of the COVID-19 Pandemic on Routine Childhood Vaccination in Low-Income Countries: A Systematic Review to Set the Scope for Future Pandemics Microorganisms (IF 4.5) Pub Date : 2024-03-13 Jessica E. Beetch, Amanda Janitz, Laura A. Beebe, Mary Gowin, Chao Xu, Shari Clifton, Katrin Gaardbo Kuhn
The COVID-19 pandemic halted progress in global vaccine coverage and disrupted routine childhood vaccination practices worldwide. While there is ample evidence of the vaccination decline experienced during the pandemic, it is less clear how low-income countries were affected. We executed a systematic review to synthesize the current literature on the impacts of routine childhood vaccinations in low-income
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Antimicrobial Resistance and Genomic Characteristics of Escherichia coli Strains Isolated from the Poultry Industry in Henan Province, China Microorganisms (IF 4.5) Pub Date : 2024-03-13 Zugang Li, Chaoying Jia, Zizhe Hu, Yancheng Jin, Tianzhi Li, Xiaoxue Zhang, Zhong Peng, Ruicheng Yang, Huanchun Chen, Xiangru Wang
Escherichia coli (E. coli) is an important foodborne pathogen and a biomarker for monitoring antimicrobial resistance. Investigating the prevalence of E. coli in the poultry industry holds great importance, particularly in Henan province, a major poultry-producing region in China. Here, we investigated the antimicrobial resistance (AMR) phenotypes of E. coli strains obtained from the poultry industry
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Mining Biosynthetic Gene Clusters of Pseudomonas vancouverensis Utilizing Whole Genome Sequencing Microorganisms (IF 4.5) Pub Date : 2024-03-09 Prabin Tamang, Arjun Upadhaya, Pradeep Paudel, Kumudini Meepagala, Charles L. Cantrell
Natural product (NP)-based pesticides have emerged as a compelling alternative to traditional chemical fungicides, attracting substantial attention within the agrochemical industry as the world is pushing toward sustainable and environmentally friendly approaches to safeguard crops. Microbes, both bacteria and fungi, are a huge source of diverse secondary metabolites with versatile applications across
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Efficacy of Entomopathogenic Staphylococcus Bacteria as a Biocontrol Agent against Rhipicephalus microplus Ticks: Assessing Reproductive Inhibition and Mortality Rates Microorganisms (IF 4.5) Pub Date : 2024-03-11 Raquel Cossio-Bayugar, Cesar A. Arreguin-Perez, Hugo Aguilar-Diaz, Estefan Miranda-Miranda
Rhipicephalus microplus is a persistent ectoparasite of cattle that causes bovine anaplasmosis and babesiosis, causing economic losses worldwide. Chemical treatment is the primary method for tick control, but the emergence of pesticide-resistant ticks is a major challenge. Alternative biocontrol strategies utilizing entomopathogenic microorganisms are being explored. This study aimed to validate the
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Research Progress on the NSP10 Protein of Porcine Reproductive and Respiratory Syndrome Virus Microorganisms (IF 4.5) Pub Date : 2024-03-11 Gan Li, Yajie Zheng, Qin Luo, Yaohua Liang, Hang Zhang, Huiyang Sha, Ruining Wang, Weili Kong, Mengmeng Zhao
Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious and pathogenic infectious disease caused by the porcine reproductive and respiratory syndrome virus (PRRSV). It manifests as reproductive disorders in sows and respiratory disorders in piglets. PRRSV infects swine herds with symptoms such as abortions, stillbirths, and mummified fetuses in gestating sows. Piglets mainly experience
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Potential Fungal Zoonotic Pathogens in Cetaceans: An Emerging Concern Microorganisms (IF 4.5) Pub Date : 2024-03-11 Victor Garcia-Bustos, Begoña Acosta-Hernández, Marta Dafne Cabañero-Navalón, Alba Cecilia Ruiz-Gaitán, Javier Pemán, Inmaculada Rosario Medina
Over 60% of emerging infectious diseases in humans are zoonotic, often originating from wild animals. This long-standing ecological phenomenon has accelerated due to human-induced environmental changes. Recent data show a significant increase in fungal infections, with 6.5 million cases annually leading to 3.7 million deaths, indicating their growing impact on global health. Despite the vast diversity
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Comparison of an Enzyme Linked-Immunosorbent Assay and a Chemiluminescent Immunoassay with an Immunofluorescence Assay for Detection of Phase II IgM and IgG Antibodies to Coxiella burnetii Microorganisms (IF 4.5) Pub Date : 2024-03-11 Juan Francisco Gutiérrez-Bautista, María Tarriño, Adrián González, María José Olivares Durán, Fernando Cobo, Juan Antonio Reguera, Javier Rodríguez-Granger, Antonio Sampedro
In this study, we have compared the detection of IgM and IgG against C. burnetii phase II of an enzyme-linked immunosorbent assay (ELISA) (Euroimmun) and a chemiluminescent immunoassay (CLIA) (VIRCLIA, Vircell). In addition, an indirect immunofluorescence assay (IFA) was used as a reference test. One hundred forty-eight sera were used for IgG evaluation, and eighty-eight for IgM. The sensitivity of
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Group A Streptococcus Pili—Roles in Pathogenesis and Potential for Vaccine Development Microorganisms (IF 4.5) Pub Date : 2024-03-11 Catherine Jia-Yun Tsai, Risa Takahashi, Jacelyn Mei-San Loh, Thomas Proft
The Gram-positive human pathogen Group A Streptococcus (GAS, Streptococcus pyogenes) employs an arsenal of virulence factors that contribute to its pathogenesis. The pilus is an important factor that enables the pathogen to adhere to and colonize host tissues. Emerging research in pilus function shows that pili’s involvement in establishing infection extends beyond host adhesion. The diversity of GAS
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Shark Microbiome Analysis Demonstrates Unique Microbial Communities in Two Distinct Mediterranean Sea Shark Species Microorganisms (IF 4.5) Pub Date : 2024-03-11 Francesco Montemagno, Chiara Romano, Deborah Bastoni, Angelina Cordone, Olga De Castro, Sergio Stefanni, Emilio Sperone, Donato Giovannelli
Our knowledge regarding the role of the microbiome in fish health has been steadily increasing in the last decade, especially for species of commercial interest. Conversely, relatively few studies focus on the microbiomes of wild fish, especially apex predators like sharks, due to lower economic interest and greater difficulty in obtaining samples. Studies investigating microbiome differences between
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Effect of Saccharina japonica Intake on Blood Pressure and Gut Microbiota Composition in Spontaneously Hypertensive Rats Microorganisms (IF 4.5) Pub Date : 2024-03-11 Ayaka Harui, Saki Maruyama, Yukiko Segawa, Nobutaka Kurihara
It was reported that the consumption of Saccharina japonica (SJ) lowers blood pressure (BP) in hypertensive rats. Hypertension is related to gut microbiota, and hypertensive patients develop dysbiosis. It was reported that the intake of dietary fiber and polysaccharides contained in SJ changes gut microbiota and increases short-chain fatty acids (SCFAs). The present study examined the effect of BP
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Developments in Negative-Strand RNA Virus Reverse Genetics Microorganisms (IF 4.5) Pub Date : 2024-03-11 Mengyi Wang, Jinyan Wu, Xiaoan Cao, Long Xu, Junhuang Wu, Haiyan Ding, Youjun Shang
Many epidemics are caused by negative-stranded RNA viruses, leading to serious disease outbreaks that threaten human life and health. These viruses also have a significant impact on animal husbandry, resulting in substantial economic losses and jeopardizing global food security and the sustainable livelihoods of farmers. However, the pathogenic and infection mechanism of most negative-stranded RNA
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Rhizosphere Microorganisms Supply Availability of Soil Nutrients and Induce Plant Defense Microorganisms (IF 4.5) Pub Date : 2024-03-11 Wannaporn Thepbandit, Dusit Athinuwat
Plant health is necessary for food security, which is a key determinant of secure and sustainable food production systems. Deficiency of soil nutrients and invasion of plant pathogens or insects are the main destroyers of the world’s food production. Synthetic fertilizers and chemical-based pesticides are frequently employed to combat the problems. However, these have negative impacts on microbial
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Early Succession of Community Structures and Biotic Interactions of Gut Microbes in Eriocheir sinensis Megalopa after Desalination Microorganisms (IF 4.5) Pub Date : 2024-03-11 Wenlei Xue, Hao Wu, Xinyu Wu, Nannan Li, Ximei Nie, Tianheng Gao
As an enduring Chinese freshwater aquaculture product, the Eriocheir sinensis has a high economic value and is characterized by a catadromous life style that undergoes seawater–freshwater migration. However, little is known about their gut microbial status as they move from saltwater to freshwater acclimatization. Here, we sampled and cultivated Eriocheir sinensis megalopa from three aquaculture desalination
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Effects of Cellulase and Xylanase on Fermentation Characteristics, Chemical Composition and Bacterial Community of the Mixed Silage of King Grass and Rice Straw Microorganisms (IF 4.5) Pub Date : 2024-03-12 Chenchen Qiu, Nanbing Liu, Xiaogao Diao, Liwen He, Hanlin Zhou, Wei Zhang
This study was to investigate the effects of cellulase and xylanase on fermentation characteristics, nutrient composition and the bacterial community of the mixed silage of king grass and rice straw. Lab-scale bag silage was produced and seven groups were studied: blank control (CK); added 1%, 2% cellulase (CE1, CE2); added 1%, 2% xylanase (XY1, XY2); and added 0.5% cellulase +0.5% xylanase, 1% cellulase
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Unveiling Shared Immune Responses in Porcine Alveolar Macrophages during ASFV and PRRSV Infection Using Single-Cell RNA-seq Microorganisms (IF 4.5) Pub Date : 2024-03-12 Bo Jiang, Lu Li, Yu Wu, Xiaoying Wang, Ning Gao, Zhichao Xu, Chunhe Guo, Sheng He, Guihong Zhang, Yaosheng Chen, Xiaohong Liu, Zhengcao Li
African swine fever virus (ASFV) and porcine reproductive and respiratory syndrome virus (PRRSV) infections lead to severe respiratory diseases in pigs, resulting in significant economic losses for the global swine industry. While numerous studies have focused on specific gene functions or pathway activities during infection, an investigation of shared immune responses in porcine alveolar macrophages
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Brevibacillus laterosporus: A Probiotic with Important Applications in Crop and Animal Production Microorganisms (IF 4.5) Pub Date : 2024-03-12 Yucheng Liu, Xueying Zai, Guangying Weng, Xianyong Ma, Dun Deng
Brevibacillus laterosporus (B. laterosporus) is widely distributed in nature and demonstrates significant potential for applications in biological control, environmental protection, agricultural production, and clinical medicine. This review provides a comprehensive overview of the applications of B. laterosporus in crop cultivation and animal feeding, as well as an examination of the antimicrobial
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Vancomycin-Resistant Streptococcus thoraltensis: A Case Report of Bacterial Endocarditis and Review of Literature on Infections Caused by This Pathogen Microorganisms (IF 4.5) Pub Date : 2024-03-12 Roxana Mihaela Chiorescu, Sandor Botond Buksa, Alexandru Botan, Mihaela Mocan, Carmen Costache, Dan Alexandru Toc
Streptococcus thoraltensis is a rare species, part of the viridans streptococcus group, found initially in rabbits and pigs, which can be vancomycin-resistant. We present the case of a 65-year-old patient, a smoker and chronic alcohol consumer with chronic obstructive pulmonary disease (COPD) and multiple dental foci who had been diagnosed with bacterial endocarditis caused by Streptococcus thoraltensis
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Immune Reactions of Vector Insects to Parasites and Pathogens Microorganisms (IF 4.5) Pub Date : 2024-03-12 Norman Arthur Ratcliffe, Cicero Brasileiro Mello, Helena Carla Castro, Paul Dyson, Marcela Figueiredo
This overview initially describes insect immune reactions and then brings together present knowledge of the interactions of vector insects with their invading parasites and pathogens. It is a way of introducing this Special Issue with subsequent papers presenting the latest details of these interactions in each particular group of vectors. Hopefully, this paper will fill a void in the literature since
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Genomic Evolution and Selective Pressure Analysis of a Novel Porcine Sapovirus in Shanghai, China Microorganisms (IF 4.5) Pub Date : 2024-03-12 Jie Tao, Benqiang Li, Ying Shi, Jinghua Cheng, Pan Tang, Jiajie Jiao, Huili Liu
Porcine sapovirus (PoSaV) is one of the most significant pathogens causing piglet diarrhea, and one with limited genetic characterization. In this study, the prevalence, infection pattern, and genetic evolution of porcine sapovirus were elucidated in detail. The positive rate of PoSaV was 10.1% (20/198), with dual, triple, and quadruple infections of 45%, 40%, and 5%, respectively. To further explore
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Unlocking the Uterine Code: Microbiota, Immune Cells, and Therapy for Recurrent Reproductive Failure Microorganisms (IF 4.5) Pub Date : 2024-03-09 Svetla Blazheva, Svetlana Pachkova, Tatyana Bodurska, Petar Ivanov, Alexander Blazhev, Tzvetan Lukanov, Emiliana Konova
The uterine microbiota has been the subject of increasing study, but its interaction with the local immune system remains unclear. Successful embryo implantation relies on endometrial receptivity, which is pivotal for immunological tolerance to fetal antigens and precise regulation of inflammatory mediators. Emerging data suggest a dynamic interplay between endometrial microflora and the immune system
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Associations of Infant Feeding, Sleep, and Weight Gain with the Toddler Gut Microbiome Microorganisms (IF 4.5) Pub Date : 2024-03-09 Magdalena Olson, Samantha Toffoli, Kiley B. Vander Wyst, Fang Zhou, Elizabeth Reifsnider, Megan E. Petrov, Corrie M. Whisner
This study examines how feeding, sleep, and growth during infancy impact the gut microbiome (GM) in toddlers. The research was conducted on toddlers (n = 36), born to Latina women of low-income with obesity. Their mothers completed retrospective feeding and sleeping questionnaires at 1, 6, and 12 months; at 36 months, fecal samples were collected. Sequencing of the 16S rRNA gene (V4 region) revealed
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A Retrospective Analysis of Salmonella Isolates across 11 Animal Species (1982–1999) Led to the First Identification of Chromosomally Encoded blaSCO-1 in the USA Microorganisms (IF 4.5) Pub Date : 2024-03-06 Nneka Vivian Iduu, Donna Raiford, Austin Conley, Joy Scaria, Julie Nelson, Laura Ruesch, Stuart Price, Min Yue, Jiansen Gong, Lanjing Wei, Chengming Wang
Antimicrobial resistance (AMR) in non-typhoidal Salmonella is a pressing public health concern in the United States, necessitating continuous surveillance. We conducted a retrospective analysis of 251 Salmonella isolates from 11 animal species recovered between 1982 and 1999, utilizing serotyping, antimicrobial susceptibility testing, and whole-genome sequencing (WGS). Phenotypic resistance was observed
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Endogenous Microbacteria Can Contribute to Ovarian Carcinogenesis by Reducing Iron Concentration in Cysts: A Pilot Study Microorganisms (IF 4.5) Pub Date : 2024-03-07 Naoki Kawahara, Shoichiro Yamanaka, Kyohei Nishikawa, Motoki Matsuoka, Tomoka Maehana, Ryuji Kawaguchi, Naoki Ozu, Tomomi Fujii, Aya Sugimoto, Akihiko Yoshizawa, Fuminori Kimura
Among epithelial ovarian cancer, clear cell carcinoma is common for chemo-resistance and high mortality. This cancer arises from benign ovarian endometrioma (OE), which is a high oxidative stress environment due to the cystic retention of menstrual blood produced during menstruation and the “iron” liberated from the cyst. There has been strong evidence that the iron concentration in OE decreases when
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SARS-CoV-2 and Other Respiratory Viruses in Human Olfactory Pathophysiology Microorganisms (IF 4.5) Pub Date : 2024-03-07 Serigne Fallou Wade, Abou Abdallah Malick Diouara, Babacar Ngom, Fatou Thiam, Ndongo Dia
Acute respiratory viruses (ARVs) are the leading cause of diseases in humans worldwide. High-risk individuals, including children and the elderly, could potentially develop severe illnesses that could result in hospitalization or death in the worst case. The most common ARVs are the Human respiratory syncytial virus, Human Metapneumovirus, Human Parainfluenza Virus, rhinovirus, coronaviruses (including
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Isolation and Characterization of a Novel Aeromonas salmonicida-Infecting Studiervirinae Bacteriophage, JELG-KS1 Microorganisms (IF 4.5) Pub Date : 2024-03-08 Karina Svanberga, Jelena Avsejenko, Juris Jansons, Davids Fridmanis, Tatjana Kazaka, Aivars Berzins, Andris Dislers, Andris Kazaks, Nikita Zrelovs
Representatives of the bacterial genus Aeromonas are some of the most notorious aquaculture pathogens associated with a range of diseases in different fish species. As the world forges toward the post-antibiotic era, alternative options for combating bacterial pathogens are needed. One such alternative option is phage biocontrol. In this study, a novel podophage—JELG-KS1—infecting Aeromonas salmonicida
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Acanthamoeba Sequence Types and Allelic Variations in Isolates from Clinical and Different Environmental Sources in Italy Microorganisms (IF 4.5) Pub Date : 2024-03-08 Federica Berrilli, Margherita Montalbano Di Filippo, Isabel Guadano-Procesi, Marta Ciavurro, David Di Cave
The genus Acanthamoeba comprises free-living amoebae distributed in a wide variety of environments. These amoebae are clinically significant, causing opportunistic infections in humans and other animals. Despite this, limited data on Acanthamoeba sequence types and alleles are available in Italy. In the present study, we analyzed all Acanthamoeba sequences deposited from Italy with new positive Acanthamoeba