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Development of a limited sampling strategy for the estimation of 1 isoniazid exposure considering N2 acetyltransferase 2 genotypes in Korean patients with tuberculosis Tuberculosis (IF 2.576) Pub Date : 2021-01-21 Jihyun Lee; Giwon Choi; Seungwon Yang; Jung-Eun Ha; Eun Sun Kim; Jong Sun Park; Kyunghoon Lee; Junghan Song; Jae Ho Lee; Jangik I. Lee
A limited sampling strategy (LSS) toate the exposure to isoniazid was developed considering N-acetyltransferase 2 (NAT2) genotypes in Korean patients with tuberculosis. The influence of NAT2 genotype on the pharmacokinetics of isoniazid was also evaluated. A total of 33 participants participated in the study and received isoniazid 300 mg once daily. Evaluable participants consist of ten slow (SA),
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Targeted next generation sequencing directly from sputum for comprehensive genetic information on drug resistant Mycobacterium tuberculosis Tuberculosis (IF 2.576) Pub Date : 2021-01-08 Priti Kambli; Kanchan Ajbani; Mubin Kazi; Meeta Sadani; Swapna Naik; Anjali Shetty; Jeffrey A. Tornheim; Harpreet Singh; Camilla Rodrigues
Background Timely drug resistance detection is essential to global tuberculosis management. Unfortunately, rapid molecular tests assess resistance to only a few drugs, with culture required for comprehensive susceptibility test results. Methods We evaluated targeted next generation sequencing (tNGS) for tuberculosis on 40 uncultured sputum samples. Resistance profiles from tNGS were compared with profiles
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Immune variations throughout the course of tuberculosis treatment and its relationship with adrenal hormone changes in HIV-1 patients co-infected with Mycobacterium tuberculosis Tuberculosis (IF 2.576) Pub Date : 2021-01-02 María Belén Vecchione; Matías Tomás Angerami; Guadalupe Verónica Suarez; Gabriela Turk; Natalia Laufer; Graciela Ben; Diego Ameri; Diego Gonzalez; Laura M. Parodi; Luis D. Giavedoni; Patricia Maidana; Bibiana Fabre; Viviana Mesch; Omar Sued; Maria Florencia Quiroga
HIV infection is a major risk factor predisposing for Mycobacterium tuberculosis infection and progression to active tuberculosis (TB). As host immune response defines the course of infection, we aimed to identify immuno-endocrine changes over six-months of anti-TB chemotherapy in HIV+ people. Plasma levels of cortisol, DHEA and DHEA-S, percentages of CD4+ regulatory T cell subsets and number of IFN-γ-secreting
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A new resource on artificial intelligence powered computer automated detection software products for tuberculosis programmes and implementers Tuberculosis (IF 2.576) Pub Date : 2021-01-04 Zhi Zhen Qin; Tasneem Naheyan; Morten Ruhwald; Claudia M. Denkinger; Sifrash Gelaw; Madlen Nash; Jacob Creswell; Sandra Vivian Kik
Recently, the number of artificial intelligence powered computer-aided detection (CAD) products that detect tuberculosis (TB)-related abnormalities from chest X-rays (CXR) available on the market has increased. Although CXR is a relatively effective and inexpensive method for TB screening and triaging, a shortage of skilled radiologists in many high TB-burden countries limits its use. CAD technology
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NIX-mediated mitophagy regulate metabolic reprogramming in phagocytic cells during mycobacterial infection Tuberculosis (IF 2.576) Pub Date : 2020-12-30 Ranjeet Singh Mahla; Akhilesh Kumar; Helena J Tutill; Sreevidhya Tarakkad Krishnaji; Bharathwaj Sathyamoorthy; Mahdad Noursadeghi; Judith Breuer; Amit Kumar Pandey; Himanshu Kumar
RNASeq analysis of PBMCs from treatment naïve TB patients and healthy controls revealed that M. tuberculosis (Mtb) infection dysregulates several metabolic pathways and upregulates BNIP3L/NIX receptor mediated mitophagy. Analysis of publicly available transcriptomic data from the NCBI-GEO database indicated that M. bovis (BCG) infection also induces similar rewiring of metabolic and mitophagy pathways
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Evaluation of CSF pyrosequencing to diagnose tuberculous meningitis: A retrospective diagnostic accuracy study Tuberculosis (IF 2.576) Pub Date : 2021-01-02 Kanchan Ajbani; Mubin Kazi; Umang Agrawal; Raj Jatale; Rajeev Soman; Ayesha Sunavala; Anjali Shetty; Camilla Rodrigues
Background We evaluated the performance of pyrosequencing, a genotypic test which detects TB and XDR-defining mutations within 6 h, directly on CSF samples for diagnosing TB meningitis(TBM). Methods This retrospective, diagnostic accuracy study was conducted in Hinduja hospital, Mumbai from May-2017 to May-2019. 107 consecutive patients with physician-suspected TBM for whom CSF pyrosequencing was requested
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Coumarin hybrid derivatives as promising leads to treat tuberculosis: Recent developments and critical aspects of structural design to exhibit anti-tubercular activity Tuberculosis (IF 2.576) Pub Date : 2021-01-06 Dinesh S. Reddy; Manasa Kongot; Amit Kumar
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Human monocyte-derived macrophage responses to M. tuberculosis differ by the host's tuberculosis, diabetes or obesity status, and are enhanced by rapamycin Tuberculosis (IF 2.576) Pub Date : 2020-12-30 Blanca I. Restrepo; Arshad Khan; Vipul K. Singh; Erica de-Leon; Génesis P. Aguillón-Durán; Eder Ledezma-Campos; David H. Canaday; Chinnaswamy Jagannath
Human macrophages play a major role in controlling tuberculosis (TB), but their anti-mycobacterial mechanisms remain unclear among individuals with metabolic alterations like obesity (TB protective) or diabetes (TB risk). To help discern this, we aimed to: i) Evaluate the impact of the host's TB status or their comorbidities on the anti-mycobacterial responses of their monocyte-derived macrophages
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Activation of hypoxia-inducible factor 1 (Hif-1) enhanced bactericidal effects of macrophages to Mycobacterium tuberculosis Tuberculosis (IF 2.576) Pub Date : 2020-12-24 Quan Li; Yuyu Xie; Zhangbo Cui; Hai Huang; Chengqing Yang; Baodong Yuan; Pei Shen; Chunwei Shi
Background Tuberculosis is chronic infection caused by Mycobacterium tuberculosis (M.tb), which infects specifically macrophages. Hif-1, hypoxia-inducible factor-1, was reported to act as master regulator of killing functions in macrophages. Aim To investigate whether Hif-1 activation would enhance bactericidal effect of macrophages and anti-tuberculosis effect of chemical reagent. Methods Hif-1 and
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Metabolic profiles of multidrug resistant and extensively drug resistant Mycobacterium tuberculosis unveiled by metabolomics Tuberculosis (IF 2.576) Pub Date : 2020-12-24 Amanda Mendes Rêgo; Duanne Alves da Silva; Nicole Victor Ferreira; Lucindo Cardoso de Pina; Joseph A.M. Evaristo; Geisa P. Caprini Evaristo; Fabio Cesar S. Nogueira; Soraya M. Ochs; Julio J. Amaral; Rosana B.R. Ferreira; L. Caetano M. Antunes
Although treatable with antibiotics, tuberculosis is a leading cause of death. Mycobacterium tuberculosis antibiotic resistance is becoming increasingly common and disease control is challenging. Conventional drug susceptibility testing takes weeks to produce results, and treatment is often initiated empirically. Therefore, new methods to determine drug susceptibility profiles are urgent. Here, we
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Proteomic profile of vitreous in patients with tubercular uveitis Tuberculosis (IF 2.576) Pub Date : 2020-12-03 Reema Bansal; Mohd M. Khan; Surendra Dasari; Indu Verma; David R. Goodlett; Nathan P. Manes; Aleksandra Nita-Lazar; Surya P. Sharma; Aman Kumar; Nirbhai Singh; Anuradha Chakraborti; Vishali Gupta; M.R. Dogra; Jagat Ram; Amod Gupta
Objective To elucidate disease-specific host protein profile in vitreous fluid of patients with intraocular inflammation due to tubercular uveitis (TBU). Methods Vitreous samples from 13 patients with TBU (group A), 7 with non-TBU (group B) and 9 with no uveitis (group C) were analysed by shotgun proteomics using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). Differentially expressed proteins
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A whole-genome sequencing study of an X-family tuberculosis outbreak focus on transmission chain along 25 years Tuberculosis (IF 2.576) Pub Date : 2020-11-28 Jessica Comín; Alberto Cebollada; Daniel Ibarz; Jesús Viñuelas; María Asunción Vitoria; María José Iglesias; Sofía Samper
Lineage 4/X-family of Mycobacterium tuberculosis is not very notorious, except for the CDC1551 strain. One strain of this family, named Ara50, caused one of the largest tuberculosis outbreaks of the Aragon region, Spain, during the 1990s and remained until 2018. These X-strains are characterised by high transmissibility and by carrying a low copy number of IS6110 in their genomes. Epidemiological data
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Verification of tuberculosis infection among Vác mummies (18th century CE, Hungary) based on lipid biomarker profiling with a new HPLC-HESI-MS approach Tuberculosis (IF 2.576) Pub Date : 2020-12-08 Orsolya Anna Váradi; Dávid Rakk; Olga Spekker; Gabriella Terhes; Edit Urbán; William Berthon; Ildikó Pap; Ildikó Szikossy; Frank Maixner; Albert Zink; Csaba Vágvölgyi; Helen D. Donoghue; David E. Minnikin; András Szekeres; György Pálfi
Tuberculosis (TB) was a large burden of infections that peaked during the 19th century in Europe. Mummies from the 18th century CE, discovered in the crypt of a church at Vác, Hungary, had high TB prevalence, as revealed by amplification of key fragments of TB DNA and genome-wide TB analysis. Complementary methods are needed to confirm these diagnoses and one approach uses the identification of specific
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Proteomic analysis reveals critical molecular mechanisms involved in the macrophage anti-spinal tuberculosis process Tuberculosis (IF 2.576) Pub Date : 2020-12-09 Guoyong Xu; Jiang Xue; Jie Jiang; Tuo Liang; Yuanlin Yao; Shian Liao; Tianyou Chen; Hao Li; Chong Liu; Xinli Zhan
Tuberculosis infection activates the autoimmune system. However, the role of host–pathogen interactions involved in Mycobacterium tuberculosis infection is unclear. In this study, we analyzed 6 spinal tuberculosis tissues and 6 herniated disc tissues by using liquid chromatography–tandem mass spectrometry coupled with tandem mass spectrometry, and immunohistochemical staining was performed for validating
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Cough-independent production of viable Mycobacterium tuberculosis in bioaerosol Tuberculosis (IF 2.576) Pub Date : 2020-12-08 Benjamin Patterson; Wayne Bryden; Charles Call; Andrea McKerry; Bryan Leonard; Ronnett Seldon; Melitta Gqada; Ryan Dinkele; Sophia Gessner; Digby F. Warner; Robin Wood
Background Symptoms of infectious respiratory illnesses are often assumed to drive transmission. However, production and release of Mycobacterium tuberculosis (Mtb) bioaerosols is poorly understood. We report quantitation of Mtb exhaled during specific respiratory manoeuvres. Methods Direct capture of nascent bioaerosol particles and indirect collection of aged particles was performed in 10 healthy
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The TB vaccine development pathway – An innovative approach to accelerating global TB vaccine development Tuberculosis (IF 2.576) Pub Date : 2020-12-08 Danielle Roordink; Ann Williams; Bernard Fritzell; Dominick J. Laddy; Emmanuelle Gerdil; Anne Marie Graffin; Dereck Tait; Leo van der Pol; Ilona van den Brink; Marit Holleman; Jelle Thole; Gerald Voss; Maria Lempicki; Georges Thiry
Two proof of concept clinical trials with TB vaccines demonstrate that new approaches can prevent sustained TB infection in adolescents (BCG revaccination) and TB disease in adults (M72/ASO1E) (Nemes et al., 2018; Tait et al., 2019) [1,2]. Both approaches are in late stage development and provide motivation and rationale to invest into a global TB vaccine pipeline. This pipeline needs to be diverse
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The association between vitamin D deficiency and extrapulmonary tuberculosis: Case-control study Tuberculosis (IF 2.576) Pub Date : 2020-12-01 Fatma Hammami; Makram Koubaa; Yosra Mejdoub; Mouna Turki; Houda Ben Ayed; Amal Chakroun; Khaoula Rekik; Fatma Smaoui; Mounir Ben Jemaa
Tuberculosis remains a public health issue worldwide. Identifying its risk factors, such as vitamin D deficiency, is mandatory so as to target the preventive strategies. We aimed to study the association between vitamin D deficiency and extrapulmonary tuberculosis. We conducted a case-control study including all cases of extrapulmonary tuberculosis hospitalized in the infectious diseases department
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Detection of mycobacteria in paraffin-embedded Ziehl–Neelsen-Stained tissues using digital pathology Tuberculosis (IF 2.576) Pub Date : 2020-11-25 Luz F. Sua; John E. Bolaños; Juliana Maya; Aura Sánchez; Giovanna Medina; Valeria Zúñiga-Restrepo; Liliana Fernández-Trujillo
Introduction Early diagnosis of paucibacillary tuberculosis represents a challenge, even with direct tissue examination. Digital pathology allows the digital analysis of tissues to identify microorganisms. We aim to develop a program to detect and quantify typical and atypical mycobacteria in paraffin-embedded Ziehl–Neelsen-stained tissues. Material and methods Program development: The building of
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Tuberculosis vaccination needs to avoid ‘decoy’ immune reactions Tuberculosis (IF 2.576) Pub Date : 2020-11-14 Juraj Ivanyi
Current search for a new effective vaccine against tuberculosis involves selected antigens, vectors and adjuvants. These are being evaluated usually by their booster inoculation following priming with Bacillus Calmette-Guerin. The purpose of this article is to point out, that despite being attenuated of virulence, priming with BCG may still involve immune mechanisms, which are not favourable for protection
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Host transcriptional response to TB preventive therapy differentiates two sub-groups of IGRA-positive individuals Tuberculosis (IF 2.576) Pub Date : 2020-11-28 Claire Broderick; Jacqueline M. Cliff; Ji-Sook Lee; Myrsini Kaforou; David AJ. Moore
We hypothesised that individuals with immunological sensitisation to Mycobacterium tuberculosis (Mtb), conventionally regarded as evidence of latent tuberculosis infection (LTBI), would demonstrate binary responses to preventive therapy (PT), reflecting the differential immunological consequences of the sterilisation of viable infection in those with active Mtb infection versus no Mtb killing in those
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Performance of a simple flow cytometric assay in diagnosing active tuberculosis Tuberculosis (IF 2.576) Pub Date : 2020-11-11 Sasiwimol Ubolyam; Thatri Iampornsin; Jiratchaya Sophonphan; Anchalee Avihingsanon; Gompol Suwanpimolkul; Kamon Kawkitinarong; Weerawat Manosuthi; Sivaporn Gatechompol; Jintanat Ananworanich; Kiat Ruxrungtham; Praphan Phanuphak; Anthony D. Kelleher
A flow cytometric assay measuring Mycobacterium tuberculosis-specific CD4 T-cell responses using co-expression of CD25/CD134 (OX40 assay) was explored as a diagnostic tool for active tuberculosis (TB) in a Thai population with and without HIV infection. Peripheral blood mononuclear cells (PBMC) obtained from 133 participants at TB diagnosis were cryopreserved. Seventy-six participants had a clinical
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Human leukocyte antigen associations with protection against tuberculosis infection and disease in human immunodeficiency virus-1 infected individuals, despite household tuberculosis exposure and immune suppression Tuberculosis (IF 2.576) Pub Date : 2020-11-19 Faheem Seedat; Ian James; Shayne Loubser; Ziyaad Waja; Simon A. Mallal; Christopher Hoffmann; Caroline T. Tiemessen; Richard E. Chaisson; Neil A. Martinson
Background To determine the association of human leukocyte antigen (HLA) alleles as correlates of risk for and protection against tuberculin skin test (TST) positivity and active TB disease amongst HIV-infected adults. Methods Genomic DNA was extracted from 754 HIV-infected adults whole-blood. HLA-A, -B, -C and -DRB1 loci were genotyped by next generation sequencing methods. HLA alleles were analysed
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Nicotine promotes the intracellular growth of Mycobacterium tuberculosis in epithelial cells Tuberculosis (IF 2.576) Pub Date : 2020-11-27 Claudia Valdez Miramontes; Adrián Rodríguez-Carlos; Sara P. Marin-Luévano; Luis A. Trejo Martínez; Jeny de Haro Acosta; José A. Enciso-Moreno; Bruno Rivas-Santiago
Several epidemiological studies have identified the cigarette smoke as a risk factor for the infection and development of tuberculosis. Nicotine is considered the main immunomodulatory molecule of the cigarette. In the present study, we evaluated the effect of nicotine in the growth of M. tuberculosis. Lung epithelial cells and macrophages were infected with M. tuberculosis and/or treated with nicotine
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Pathology of TB/COVID-19 Co-Infection: The phantom menace Tuberculosis (IF 2.576) Pub Date : 2020-11-17 Gabriel Tassi Mousquer; Alessandra Peres; Marilu Fiegenbaum
Tuberculosis (TB) and coronavirus disease 2019 (COVID-19) are currently the two main causes of death among infectious diseases. There is an increasing number of studies trying to elucidate the interactions between Mycobacterium tuberculosis and SARS-CoV-2. Some of the first case reports point to a worsening of respiratory symptoms in co-infected TB/COVID-19 individuals. However, data from the cohort
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Ubiquitous and multifaceted: SIT53 spoligotype does not correlate with any particular family of Mycobacterium tuberculosis Tuberculosis (IF 2.576) Pub Date : 2020-11-19 Igor Mokrousov
Euro-American lineage (Lineage 4) of Mycobacterium tuberculosis comprises genetically and geographically diverse families that differ in their clinical and/or epidemiological capacities. Due to the characteristic structure of the CRISPR locus (presence of almost all 43 classical spacers except for deleted signals 33–36), spoligotype SIT53 takes the basal position in the evolution of this lineage. In
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Insights into the molecular pathogenesis of ocular tuberculosis Tuberculosis (IF 2.576) Pub Date : 2020-11-12 Rupesh Agrawal; Ilaria Testi; Andres Rousselot; Elizabeth J. Chen; Rajamani Lakshminarayanan; Amit Singhal; Manish Bundele; Paul Hutchison; Onn Min Kon; Vishali Gupta; Carlos Pavesio
Unclear pathogenic mechanisms underlying the ocular tuberculosis (OTB) has resulted in perplexity related to the diagnosis and management of the disease. Developments in experimental research and innovations in molecular diagnostics have recently provided a new understanding of disease pathogenesis and natural history. The current review focuses on the new insights into OTB pathogenesis, derived from
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Cerebrospinal fluid IL-1β is elevated in tuberculous meningitis patients but not associated with mortality Tuberculosis (IF 2.576) Pub Date : 2020-11-11 Valerie A.C.M. Koeken; Ahmad R. Ganiem; Sofiati Dian; Rovina Ruslami; Lidya Chaidir; Mihai G. Netea; Vinod Kumar; Bachti Alisjahbana; Reinout van Crevel; Arjan van Laarhoven
Inflammation contributes to the pathophysiology and high mortality of tuberculous meningitis. The IL-1β pathway has been implicated in immunopathology and could be a target for host-directed therapy. IL-1β was elevated in the cerebrospinal fluid (CSF) of 225 HIV-uninfected tuberculous meningitis patients in Indonesia compared to controls, but did not predict subsequent mortality, nor did IL-6 or IL-1Ra
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Investigating extradomiciliary transmission of tuberculosis: An exploratory approach using social network patterns of TB cases and controls and the genotyping of Mycobacterium tuberculosis Tuberculosis (IF 2.576) Pub Date : 2020-10-24 Suani T.R. Pinho; Susan M. Pereira; José G.V. Miranda; Tonya A. Duarte; Joilda S. Nery; Maeli G. de Oliveira; M. Yana G.S. Freitas; Naila A. De Almeida; Fabio B. Moreira; Raoni B.C. Gomes; Ligia Kerr; Carl Kendall; M. Gabriela M. Gomes; Theolis C.B. Bessa; Roberto F.S. Andrade; Mauricio L. Barreto
Extradomiciliary contacts have been overlooked in the study of TB transmission due to difficulties in identifying actual contacts in large populations. Complex network analysis provides a framework to model the structure of contacts, specially extradomiciliary ones. We conducted a study of incident sputum-positive TB cases and healthy controls occurring in a moderate TB burden city. Cases and controls
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Evaluation of genotype MTBDRplus V2 and genotype MTBDRsl V2 for the diagnosis of extrapulmonary tuberculosis in India Tuberculosis (IF 2.576) Pub Date : 2020-10-23 Binit Kumar Singh; Rohini Sharma; Jigyasa Chaubey; Nitin Gupta; Manish Soneja; Pankaj Jorwal; Neeraj Nischal; Ashutosh Biswas; Naveet Wig; Sanjay Sarin; Ranjani Ramachandran
Microbiological diagnosis of extra-pulmonary tuberculosis (EPTB) has been one of the most difficult aspects of tuberculosis (TB) management. Availability of better imaging and diagnostic modalities has led to an increase in the number of diagnosed cases. The current upsurge in multidrug-resistant tuberculosis warrants routine testing of EPTB samples for resistance at baseline with shorter turn-around
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A highly rifampicin resistant Mycobacterium tuberculosis strain emerging in Southern Brazil Tuberculosis (IF 2.576) Pub Date : 2020-10-30 Maria Lucia Rossetti; Pedro Eduardo Almeida da Silva; Richard Steiner Salvato; Ana Júlia Reis; Sun Hee Schiefelbein; Andrea von Groll; Regina Bones Barcellos; Raquel Maschmann; Leonardo Souza Esteves; Fernanda Spies; Rubia Raubach Trespach; Elis Regina Dalla Costa; Hermes Luís Neubauer de Amorim
Here we described phenotypical, molecular and epidemiological features of a highly rifampicin-resistant Mycobacterium tuberculosis strain emerging in Southern Brazil, that carries an uncommon insertion of 12 nucleotides at the codon 435 in the rpoB gene. Employing a whole-genome sequencing-based study on drug-resistant Mycobacterium tuberculosis strains, we identified this emergent strain in 16 (9
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Population structure of multidrug-resistant Mycobacterium tuberculosis clinical isolates in Colombia Tuberculosis (IF 2.576) Pub Date : 2020-10-17 Juan Germán Rodríguez-Castillo; Claudia Llerena; Lorena Argoty-Chamorro; Julio Guerra; David Couvin; Nalin Rastogi; Martha Isabel Murcia
Emergence of multidrug-resistant (MDR) Mycobacterium tuberculosis complex (MTBC) isolates is a major public health problem that threatens progress made in tuberculosis (TB) care and control worldwide. In Colombia, the prevalence of MDR tuberculosis (MDR-TB) has increased slowly but steadily since 2001. However, the population structure of the MDR-TB strains circulating in Colombia is sparsely known
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Pyrazinamide resistance and pncA mutations in drug resistant Mycobacterium tuberculosis clinical isolates from Myanmar Tuberculosis (IF 2.576) Pub Date : 2020-10-27 Phyu Win Ei; Aye Su Mon; Mi Mi Htwe; Su Mon Win; Kay Thi Aye; Lai Lai San; Ni Ni Zaw; Wint Wint Nyunt; Zaw Myint; Jong Seok Lee; Wah Wah Aung
Pyrazinamide (PZA) is an important anti-tuberculosis drug, which is active against semi-dormant bacilli and used as a component of first-line drugs and drug-resistant tuberculosis regimens. Mutations in pncA and its promoter region are main cause of PZA resistance. There are limited PZA susceptibility data as there is no routine drug susceptibility testing (DST) for PZA. This study was aimed to determine
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The immunological architecture of granulomatous inflammation in central nervous system tuberculosis Tuberculosis (IF 2.576) Pub Date : 2020-10-22 Stefan-Dan Zaharie; Daniel J. Franken; Martijn van der Kuip; Sabine van Elsland; Bernadette S. de Bakker; Jaco Hagoort; Sanna L. Roest; Carmen S. van Dam; Carlie Timmers; Regan Solomons; Ronald van Toorn; Mariana Kruger; A. Marceline van Furth
Of all tuberculosis (TB) cases, 1% affects the central nervous system (CNS), with a mortality rate of up to 60%. Our aim is to fill the ‘key gap’ in TBM research by analyzing brain specimens in a unique historical cohort of 84 patients, focusing on granuloma formation. We describe three different types: non-necrotizing, necrotizing gummatous, and necrotizing abscess type granuloma. Our hypothesis is
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Identification of a Mycobacterium tuberculosis-specific gene marker for diagnosis of tuberculosis using semi-nested melt-MAMA qPCR (lprM-MAMA) Tuberculosis (IF 2.576) Pub Date : 2020-09-28 Kai Ling Chin; Maria E. Sarmiento; Zainal Arifin Mustapha; Jaeyres Jani; Norfazirah Binti Jamal; Cheronie Shely Stanis; Armando Acosta
Tuberculosis (TB) is the deadliest of infectious diseases. TB diagnosis, based on sputum microscopy, culture, and nucleic acid amplification tests (NAATs) to identify its main causative agent, Mycobacterium tuberculosis (MTB), remains challenging. The current available NAATs, endorsed by World Health Organization (WHO), can differentiate MTB from some MTB complex (MTBC) members. Using bioinformatics
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Loss of T cells expressing CD27 at the site of active tuberculosis – A prospective diagnostic study Tuberculosis (IF 2.576) Pub Date : 2020-10-19 Catharina Müller; Rudolf Rumetshofer; Heide-Maria Winkler; Manuel Bécède; Meinhard Kneussl; Stefan Winkler
The lack of a rapid and reliable diagnostic test for active tuberculosis is still a burden to the control of the infection. The accumulation of Mycobacterium tuberculosis (MTB)-specific CD4+ T cells at the site of infection and the increase of MTB-specific CD27− cells seem to be characteristic for active tuberculosis. We evaluated CD27 expression of non-stimulated T cells at the site of infection compared
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Performance of GeneXpert ultra in the diagnosis of Tuberculous Cervical lymphadenitis in formalin fixed paraffin embedded tissues Tuberculosis (IF 2.576) Pub Date : 2020-10-24 Emna Romdhane; Amira Arfaoui; Chaouki Benabdessalem; Meriam Ksentini; Asma Ferjani; Neira Dekhil; Rim Lahiani; Soumaya Bchiri; Helmi Mardassi; Mohamed-Ridha Barbouche; Ilhem Boutiba Ben Boubake; Mamia Ben Salah; Soumaya Rammeh
The diagnosis of Tuberculous Cervical lymphadenitis (TCL) is challenging. The present study aimed to assess the performance of GeneXpert ultra (GXu) in the diagnosis of TCL on Formalin Fixed, Paraffin Embedded Tissues (FFPET). This study included 35 TCL cases confirmed by positive microbiology and/or positive GXu on Fresh Tissues (FT). The diagnostic performance parameters of GXu on FFPET were determined
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Solid lipid nanoparticles containing anti-tubercular drugs attenuate the Mycobacterium marinum infection Tuberculosis (IF 2.576) Pub Date : 2020-10-10 Sunil Khatak; Meenu Mehta; Rajendra Awasthi; Keshav Raj Paudel; Sachin Kumar Singh; Monica Gulati; Nicole G. Hansbro; Philip M. Hansbro; Kamal Dua; Harish Dureja
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The effect of micro-nutrients on malnutrition, immunity and therapeutic effect in patients with pulmonary tuberculosis: A systematic review and meta‐analysis of randomised controlled trials Tuberculosis (IF 2.576) Pub Date : 2020-09-29 Haiqing Cai; Lulin Chen; Chunlian Yin; Yanying Liao; Xingxing Meng; Cailing Lu; Shen Tang; Xiyi Li; Xinhang Wang
Objective Micro-nutrients are closely related to pulmonary tuberculosis (PTB). Most patients with PTB suffer from micro-nutrients deficiency. We aimed to evaluate the efficacy of micro-nutrients support on clinical therapy and chronic inflammation in patients with PTB. Methods We searched Pubmed, Springer link, Web of Science, Cochrane, Wan Fang and CNKI databases for randomised controlled trials (RCTs)
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Laboratory development of a simple stool sample processing method diagnosis of pediatric tuberculosis using Xpert Ultra Tuberculosis (IF 2.576) Pub Date : 2020-10-03 Manon Lounnas; Abibatou Diack; Mark P. Nicol; Sara Eyangoh; Eric Wobudeya; Olivier Marcy; Sylvain Godreuil; Maryline Bonnet
Stool samples are alternatives to respiratory samples for bacteriological confirmation of childhood tuberculosis but require intensive laboratory processing before molecular testing to remove PCR inhibitors and debris. We aimed to develop a centrifuge-free processing method for use in resource-limited settings based on a sucrose-flotation method that showed good sensitivity for childhood tuberculosis
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Modulation of the M. tuberculosis cell envelope between replicating and non-replicating persistent bacteria Tuberculosis (IF 2.576) Pub Date : 2020-10-05 Haley Stokas; Heather L. Rhodes; Georgiana E. Purdy
The success of Mycobacterium tuberculosis as a human pathogen depends on the bacterium's ability to persist in a quiescent form in oxygen and nutrient-poor host environments. In vitro studies have demonstrated that these restricting environments induce a shift from bacterial replication to non-replicating persistence (NRP). Entry into NRP involves changes in bacterial metabolism and remodeling of the
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Mycobacterium bovis and you: A comprehensive look at the bacteria, its similarities to Mycobacterium tuberculosis, and its relationship with human disease Tuberculosis (IF 2.576) Pub Date : 2020-10-02 Carly Kanipe; Mitchell V. Palmer
While Mycobacterium tuberculosis is the primary cause of tuberculosis in people, multiple other mycobacteria are capable of doing so. With the World Health Organization's goal of a 90% reduction in tuberculosis by 2035, all tuberculous mycobacteria need to be addressed. Understanding not only the similarities, but importantly the differences between the different species is crucial if eradication is
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Overexpression of the celA1 gene in BCG modifies surface pellicle, glucosamine content in biofilms, and affects in vivo replication Tuberculosis (IF 2.576) Pub Date : 2020-10-01 Alonso Vaca-González; Mario Alberto Flores-Valdez; Michel de Jesús Aceves-Sánchez; Tanya Amanda Camacho-Villegas; Nayeli Areli Pérez-Padilla; Mirna Burciaga-Flores; Miguel Ángel De la Cruz; Miguel A. Ares; Héctor Manuel Mora-Montes; Jorge Bravo-Madrigal; Jorge Gaona-Bernal; Alma Karina Tamez-Castrellón
Biofilm formed in vitro by mycobacteria has been associated with increased antibiotic tolerance as compared with planktonic cells. Cellulose has been identified as a component of DTT-exposed biofilms formed by M. tuberculosis. The celA1 gene of M. tuberculosis encodes a cellulase, which could affect the formation of biofilm by slow-growing mycobacteria. In this work, the celA1 gene of M. tuberculosis
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Transmission of Mycobacterium tuberculosis presenting unusually high discordance between genotypic and phenotypic resistance to rifampicin in an endemic tuberculosis setting Tuberculosis (IF 2.576) Pub Date : 2020-09-28 Angela Pires Brandao; Juliana Maira Watanabe Pinhata; Vera Simonsen; Rosangela Siqueira Oliveira; Kelen Teixeira Ghisi; Michelle Christiane Silva Rabello; Suely Fukasava; Lucilaine Ferrazoli
Background Since the implementation of the Xpert MTB/RIF in Sao Paulo, Brazil, numerous Mycobacterium tuberculosis isolates presenting “rifampicin-resistant genotype with rifampicin-susceptible phenotype” were observed. Objective To evaluate the prevalence, rpoB mutations and transmission of M. tuberculosis resistant to rifampicin on Xpert MTB/RIF but susceptible on BACTEC MGIT system, in Sao Paulo
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Machine-assisted interpretation of auramine stains substantially increases through-put and sensitivity of microscopic tuberculosis diagnosis Tuberculosis (IF 2.576) Pub Date : 2020-09-19 L. Horvath; S. Hänselmann; H. Mannsperger; S. Degenhardt; K. Last; S. Zimmermann; I. Burckhardt
Of all bacterial infectious diseases, infection by Mycobacterium tuberculosis poses one of the highest morbidity and mortality burdens on humans throughout the world. Due to its speed and cost-efficiency, manual microscopy of auramine-stained sputum smears remains a crucial first-line detection method. However, it puts considerable workload on laboratory staff and suffers from a limited sensitivity
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Oldest evidence of tuberculosis in Argentina: A multidisciplinary investigation in an adult male skeleton from Saujil, Tinogasta, Catamarca (905–1030 CE) Tuberculosis (IF 2.576) Pub Date : 2020-09-22 Leandro Hernán Luna; Claudia Marcela Aranda; Ana Luisa Santos; Helen D. Donoghue; Oona Ying-Chi Lee; Houdini Ho Tin Wu; Gurdyal Singh Besra; David Ernest Minnikin; Gareth Llewellyn; Christopher Matthew Williams; Norma Ratto
The Mycobacterium tuberculosis complex (MTC) has affected South American populations since ca. 200 years BCE. In Argentina, possible cases date from ca. 1000–1400 Common Era (CE). This paper describes the oldest (905–1030 CE) confirmed case of tuberculosis (TB) in a young adult male from Lomitas de Saujil (Tinogasta, Catamarca, Argentina). Osteolytic lesions on the bodies of the lower spine were macroscopically
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Current advances in the clinical development of anti-tubercular agents. Tuberculosis (IF 2.576) Pub Date : 2020-09-05 Samanvai Reddy Tetali,Eswar Kunapaeddi,Raghu Prasad Mailavaram,Vinayak Singh,Pobitra Borah,Pran Kishore Deb,Katharigatta N Venugopala,Wafa Hourani,Rakesh Kumar Tekade
Tuberculosis (TB) is a communicable airborne infectious disease caused by the Mycobacterium tuberculosis (MTB) that primarily affects the lungs, and can disseminate to other parts of the body. MTB is one of the most dangerous pathogens, killing about 1.4 million people annually worldwide. Although the standard treatment of TB is comprised of four anti-TB drugs, the emergence of multidrug-resistant
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Identification of a novel biomarker based on lymphocyte count, albumin level, and TBAg/PHA ratio for differentiation between active and latent tuberculosis infection in Japan. Tuberculosis (IF 2.576) Pub Date : 2020-09-11 Seigo Katakura,Nobuaki Kobayashi,Hisashi Hashimoto,Chisato Kamimaki,Katsushi Tanaka,Sousuke Kubo,Kentaro Nakashima,Shuhei Teranishi,Keisuke Watanabe,Yu Hara,Masaki Yamamoto,Makoto Kudo,Hongmei Piao,Takeshi Kaneko
Data from China have shown that the ratio of Mycobacterium tuberculosis-specific antigen (TBAg) spots obtained from the T-SPOT.TB test to the number of positive control phytohemagglutinin (PHA) spots (TBAg/PHA ratio) could help distinguish between active tuberculosis infection (ATBI) and latent tuberculosis infection (LTBI). As the applicability of the T-SPOT.TB test may differ according to region
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Risk factors associated with large clusters of tuberculosis patients determined by whole-genome sequencing in a high-tuberculosis-burden country. Tuberculosis (IF 2.576) Pub Date : 2020-09-11 Reiko Miyahara,Nat Smittipat,Tada Juthayothin,Hideki Yanai,Areeya Disratthakit,Worarat Imsanguan,Daranee Intralawan,Supalert Nedsuwan,Boonchai Chaiyasirinroje,Surasit Bupachat,Katsushi Tokunaga,Surakameth Mahasirimongkol,Prasit Palittapongarnpim
Whole-genome sequencing (WGS) analysis has great discriminative power for detecting similar molecular fingerprints of suspected tuberculosis (TB) clusters. The proportion of TB cases within clusters and the associated risk factors are important epidemiological parameters guiding appropriate outbreak control strategies in endemic settings. We conducted a hospital-based TB case-cohort study between 2003
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Xpert MTB/RIF ultra for the diagnosis of tuberculous meningitis: A diagnostic accuracy study from India. Tuberculosis (IF 2.576) Pub Date : 2020-09-07 Kusum Sharma,Megha Sharma,Ritu Shree,Manish Modi,Manoj Goyal,Dhawal Narang,Aman Sharma,Vivek Lal,Navneet Sharma,Pallab Ray
Setting Studies evaluating Xpert MTB/RIF Ultra assay (Xpert Ultra) for diagnosing tuberculous meningitis (TBM) are scarce and have reported contrasting results in comparison to Xpert MTB/RIF assay (Xpert). Objective To evaluate the performance of Xpert Ultra in diagnosing TBM and compare it with Xpert and multiplex polymerase chain reaction (MPCR). Design Xpert Ultra was performed on 244 cerebrospinal
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Diagnostic performance of real time PCR and MALDI-TOF in the detection of nontuberculous mycobacteria from clinical isolates. Tuberculosis (IF 2.576) Pub Date : 2020-09-01 Ellappan Kalaiarasan,Kalpana Thangavelu,Krishnakumariamma Krishnapriya,Muthaiah Muthuraj,Maria Jose,Noyal Mariya Joseph
This study aimed to evaluate the performance of real-time PCR (qPCR) and MALDI-TOF for accurate and timely detection of nontuberculous mycobacterium (NTM) from clinical isolates. We collected fifty NTM suspected Mycobacteria Growth Indicator Tube (MGIT) cultures and analysed the diagnostic performance of qPCR and VITEK MS using Line Probe Assay (LPA) GenoType CM (Common Mycobacteria) as gold standard
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Three promising antimycobacterial medicinal plants reviewed as potential sources of drug hit candidates against multidrug-resistant tuberculosis. Tuberculosis (IF 2.576) Pub Date : 2020-08-16 Naasson Tuyiringire,Serawit Deyno,Anke Weisheit,Casim Umba Tolo,Deusdedit Tusubira,Jean-Pierre Munyampundu,Patrick Engeu Ogwang,Claude Mambo Muvunyi,Yvan Vander Heyden
Regimens of current drugs for tuberculosis are lengthy and are associated with many adverse effects. Currently, the emergence of different resistant strains has been observed. This urges a need for the discovery and development of novel drugs. The main sources of drug lead candidates are based on natural products. Zanthoxylum leprieurii, Lantana camara, and Cryptolepis Sanguinolenta are among the plants
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A novel mycobacterial growth inhibition assay employing live-cell imaging of virulent M. tuberculosis and monitoring of host cell viability. Tuberculosis (IF 2.576) Pub Date : 2020-08-14 Blanka Andersson,Michaela Jonsson Nordvall,Amanda Welin,Maria Lerm,Thomas Schön
Our aim was to develop a Mycobacterium tuberculosis (Mtb) growth inhibition assay (MGIA) as a summary estimate of host immune control of virulent Mtb. Mycobacterial growth inhibition (MGI) using previously frozen human PBMCs infected with H37Rv was assessed by live-cell imaging (Incucyte©) complemented by imaging flow cytometry analysis of phagocytosis. MGI measured as relative fluorescence units (RFU)
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Recent advances in the synthesis of Quinazoline analogues as Anti-TB agents. Tuberculosis (IF 2.576) Pub Date : 2020-08-13 Apurba Dutta,Diganta Sarma
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Alternative biomarkers for classification of latent tuberculosis infection status in pregnant women with borderline Quantiferon plus results. Tuberculosis (IF 2.576) Pub Date : 2020-08-12 Fregenet Tesfaye,Erik Sturegård,John Walles,Niclas Winqvist,Taye Tolera Balcha,Sara Karlson,Daba Mulleta,Per-Erik Isberg,Marianne Jansson,Per Björkman
Borderline interferon-gamma (IFN-γ) results (near the cut-off level 0.35 IU/ml) occur in QuantiFERON (QFT) assays. We investigated the performance of alternative biomarkers for classification of latent tuberculosis infection (LTBI) status in pregnant women with borderline QFT IFN-γ responses. Pregnant women (n = 96) were identified from a cohort study in Ethiopia, based on QFT-Plus IFN-γ results (QFT-low:
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Efficient genome editing in pathogenic mycobacteria using Streptococcus thermophilus CRISPR1-Cas9. Tuberculosis (IF 2.576) Pub Date : 2020-08-12 Aniek S Meijers,Ran Troost,Roy Ummels,Janneke Maaskant,Alexander Speer,Sergey Nejentsev,Wilbert Bitter,Coenraad P Kuijl
The ability to genetically engineer pathogenic mycobacteria has increased significantly over the last decades due to the generation of new molecular tools. Recently, the application of the Streptococcus pyogenes and the Streptococcus thermophilus CRISPR‐Cas9 systems in mycobacteria has enabled gene editing and efficient CRISPR interference‐mediated transcriptional regulation. Here, we converted CRISPR
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A population approach of rifampicin pharmacogenetics and pharmacokinetics in Mexican patients with tuberculosis. Tuberculosis (IF 2.576) Pub Date : 2020-08-08 Susanna Edith Medellin-Garibay,Ana Patricia Huerta-García,Ana Socorro Rodríguez-Báez,Martín Magaña-Aquino,Arturo Ortiz-Álvarez,Diana Patricia Portales-Pérez,Rosa Del Carmen Milán-Segovia,Silvia Romano-Moreno
The aim of this study was to develop a population pharmacokinetic model of rifampicin (RMP) in Mexican patients with tuberculosis (TB) to evaluate the influence of anthropometric and clinical covariates, as well as genotypic variants associated with MDR1 and OATP1B1 transporters. A prospective study approved by Research Ethics Committee was performed at Hospital Central in San Luis Potosí, Mexico.
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NOTCH1 and DLL4 are involved in the human tuberculosis progression and immune response activation. Tuberculosis (IF 2.576) Pub Date : 2020-08-08 Ricardo C Castro,Fabiana A Zambuzi,Caroline Fontanari,Fabiana R de Morais,Valdes R Bollela,Steven L Kunkel,Matthew A Schaller,Fabiani G Frantz
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Molecular analysis of streptomycin-resistance associating genes in Mycobacterium tuberculosis isolates from Nepal. Tuberculosis (IF 2.576) Pub Date : 2020-08-08 Dipti Shrestha,Bhagwan Maharjan,Nan Aye Thida Oo,Norikazu Isoda,Chie Nakajima,Yasuhiko Suzuki
Mutation in rpsL (encoding ribosomal protein S12), rrs (encoding 16S ribosomal RNA) and gidB (encoding 7-methylguanosine methyltransferase) are associated with resistance to