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The Xmas-2 Homologues, the Main Component of the TREX-2 mRNA Export Complex Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-12-25 E. N. Nabirochkina, D. V. Kopytova
Abstract TREX-2 complex is responsible for general mRNA export from nucleus to cytoplasm in eukaryote. The main protein of TREX-2 complex of D. melanogaster is protein Xmas-2. Its homologues in yeast and humans are Sac3 and GANP proteins, respectively. All three proteins contain the highly conserved domain Sac3-GANP, which is essential for interaction of TREX-2 complex with mRNA and another protein
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New Sea Anemone Toxin RTX-VI Selectively Modulates Voltage-Gated Sodium Channels Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-12-25 R. S. Kalina, S. Peigneur, I. N. Gladkikh, P. S. Dmitrenok, N. Y. Kim, E. V. Leychenko, M. M. Monastyrnaya, J. Tytgat, E. P. Kozlovskaya
Abstract A new neurotoxin RTX-VI that modulates the voltage-gated sodium channels (NaV) was isolated from the ethanolic extract of the sea anemone Heteractis crispa. Its amino acid sequence was determined using the combination of Edman degradation and tandem mass spectrometry. RTX-VI turned out to be an unusual natural analogue of the previously described sea anemone toxin RTX-III. The RTX-VI molecule
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Adaptation of Mycoplasmas to Antimicrobial Peptides: Development of Melittin Resistance in Acholeplasma laidlawii Is Associated with Changes in Genomic and Proteomic Profiles and in Virulence Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-12-25 V. M. Chernov, N. B. Baranova, A. A. Mouzykantov, E. S. Medvedeva, M. A. Dramchini, O. A. Chernova
Abstract For the first time it is shown that the development of resistance to melittin in Acholeplasma laidlawii, a mycoplasma that is widely spread in nature and that is the main contaminant of cell cultures and vaccines, is associated with significant changes in the genomic profile, in cellular and vesicular proteomes, as well as in virulence.
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Growth-Stimulating Activity of Natural Polymer-Based Nanocomposites of Selenium during the Germination of Cultivated Plant Seeds Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-12-25 V. N. Nurminsky, A. I. Perfileva, I. S. Kapustina, I. A. Graskova, B. G. Sukhov, B. A. Trofimov
Abstract The growth-stimulating activity of three selenium nanocomposites (NCs) in various matrices based on arabinogalactan (NC Se/AG, 6.4% Se), starch (NC Se/St, 2% Se), and carrageenan (NC Se/Car, 12% Se) with respect to plants of radish, soybean, and potato was investigated. It was shown that the treatment of plant seeds with NCs stimulated root growth during germination. It was found that the
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Biomineralization of Trabecular Meshwork in Open-Angle Glaucoma Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-12-25 M. V. Kravchik, A. M. Subbot, I. A. Novikov, A. A. Antonov, S. Yu. Petrov, S. E. Avetisov
Abstract A new form of open-angle glaucoma has been identified, in which calcification and silicification of the trabecular meshwork is a potentially significant component of outflow obstruction. It is noted that the mineralization of this area is promoted by various disturbances in the acid–base balance in the tissue. The role of melanosomal enzymes in the initiation of the formation of mineral calcium
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Computer Modeling of N-Acetylglutamate Synthase: From Primary Structure to Elemental Stages of Catalysis Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-12-25 I. V. Polyakov, A. E. Kniga, B. L. Grigorenko, A. V. Nemukhin, S. D. Varfolomeev
Abstract Three-dimensional full-atom model of the enzyme complex with acetyl-CoA and substrate was constructed on the basis of the primary sequence of amino acid residues of N-acetyl glutamate synthase. Bioinformatics approaches of computer modeling were applied, including multiple sequence alignment, prediction of co-evolutionary contacts, and ab initio folding. On the basis of the results of calculations
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Inhibition of Histone Deacetylases 4 and 5 Reduces Titin Proteolysis and Prevents Reduction of TTN Gene Expression in Atrophied Rat Soleus Muscle after Seven-Day Hindlimb Unloading Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-12-25 Yu. V. Gritsyna, A. D. Ulanova, S. S. Popova, A. G. Bobylev, V. K. Zhalimov, T. L. Nemirovskaya, B. S. Shenkman, I. M. Vikhlyantsev
Abstract The effect of HDACs 4 and 5 on the level of atrophy, calpain-1 and titin content, and TTN gene expression in rat soleus after 7-day gravitational unloading (hindlimb suspension model) was studied. The development of atrophic changes induced by gravitational unloading in rat soleus was accompanied by an increase in the calpain-1 content, an increase in titin proteolysis, and a decrease in the
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Influence of HLDF Differentiation Factor on Nonspecific Invasive Breast Carcinoma in vitro Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-12-25 A. I. Autenshlyus, I. P. Zhurakovsky, K. I. Davletova, A. P. Bogachuk, V. V. Lyakhovich, V. M. Lipkin
Abstract The study was carried out on samples of invasive breast carcinoma of no special type from 36 patients aged 48.0 to 62.8 years. The effect of HLDF on nonspecific invasive breast carcinoma was a decrease in the relative content of low-differentiated cells and an increase in the relative content of highly differentiated cells. HLDF did not have a cytotoxic effect leading to the death of low-differentiated
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Depolarization of the Rat Atria in Experimental Simulation of the Holiday Heart Syndrome Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-12-25 S. L. Smirnova, I. M. Roshchevskaya, V. N. Stolyaruk, M. B. Vititnova, I. B. Tsorin, S. A. Kryzhanovsky
Abstract In the study of the sequence of depolarization of the atrial subepicardium of rats in the short-term alcohol consumption model (the “Holiday heart” syndrome), the localization of the sources of atrial arrhythmias was determined for the first time. The difference in the excitation of the right and left atria was discovered: the right atrium is activated anterogradely from the sinoatrial node
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Melatonin Supports Photochemical Activity of Assimilation Apparatus and Delays Senescence of Leaves of Monocotyledonous Plants Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-12-25 E. D. Danilova, M. V. Efimova, L. V. Kolomeichuk, V. V. Kuznetsov
Abstract Melatonin supports the photochemical activity of photosystem II (PS II) and slows down the degradation of the main photosynthetic pigments during aging of leaves of monocotyledonous plants. The protective effect of melatonin is manifested in an increase in the maximum (Fv/Fm) and effective (Y(II)) quantum yield of PS II, in an increase of regulated (Y(NPQ)) and a decrease in unregulated dissipation
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Indirubin-3′-Oxime (IDR3O) Inhibits Proliferation of Osteosarcoma Cells in vitro and Tumor Growth in vivo Through AMPK-Activation and PGC-1α/TFAM Up-Regulation Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-12-25 Bing Fu, Guorui Yin, Ke Song, Xiurui Mu, Bo Xu, Xubin Zhang
Abstract Osteosarcoma, a malignant tumor of bones, has very high incidence in adolescents and young people. The present study investigated the effect of indirubin-3′-oxime (IDR3O) derivative on proliferation of osteosarcoma cells in vitro and tumor growth in vivo. Changes in growth and induction of apoptosis in osteosarcoma cells were assessed using WST-8 and TUNEL staining assays. Treatment of MG63
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Effects of Silicon Nanoparticles on Photosynthetic Pigments and Biogenic Elements in Tomato Plants Infected with Root-Knot Nematode Meloidogyne incognita Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-12-25 Zh. V. Udalova, G. E. Folmanis, M. A. Fedotov, L. A. Pelgunova, E. Yu. Krysanov, F. K. Khasanov, S. V. Zinovieva
Abstract New data on the effect of colloidal silicon nanoparticles on the content of nutrients and photosynthetic pigments in tomato plants invaded by root-knot nematode Meloidogyne incognita are presented. Foliar treatment of plants with colloidal solutions of silicon nanoparticles at concentrations of 0.5 and 1.0 μg/mL revealed an increase in the content of photosynthetic pigments and a number of
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Fatty Acids Distribution in Seston, Tissues, and Faecal Pellets of Blue Mussels Mytilus edulis L. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-12-25 N. N. Fokina, T. R. Ruokolainen, N. N. Nemova, D. M. Martynova, A. A. Sukhotin
Abstract The n-3 polyenoic fatty acids (phytoplankton origin) dominate in the fatty acid composition of seston, which is a food source for bivalves; this indicates the predominance of diatoms in the seston composition. The distribution of the main classes of lipids and their fatty acids in the tissues of blue mussels depends on the mollusk body size. As the body size of the mollusk increases, the ratio
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Prostate Cancer Growth Inhibition by 1-(3,5-Dimethylphenyl)–6-methyl-1H-pyrazolo[4,3-c]pyridin-4(5H)-one via Down-regulation of Phosphorylation PI3K/AKT and STA3/JAK2 Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-12-25 Jingxin Xue, Zhenwei Zhang, Heyi Hu
Abstract In the present study, 1-(3,5-dimethylphenyl)-6-methyl-1H-pyrazolo[4,3-c]pyridin-4 (5H)-one (DPMPP) was investigated as an antiproliferative agent for prostate cancer cells and the mechanism of its action was studied. Cell lines 22Rv1 and SGC‑7901 were used as in vitro models of prostate cancer. The DPMPP treatment inhibited proliferation of 22Rv1 and SGC‑7901 cells in dose-depended manner
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The Nature of Resistance of the Coagulation Factor XIII Structure to Hypochlorite-Induced Oxidation Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-12-25 A. D. Vasilyeva, L. V. Yurina, A. E. Bugrova, M. I. Indeykina, A. S. Kononikhin, A. N. Schegolikhin, V. S. Ivanov, E. N. Nikolaev, M. A. Rosenfeld
Abstract The damage to blood coagulation factor XIII (FXIII) at different stages of its enzymatic activation under the action of various physiological amounts of hypochlorite ion was studied. The results obtained by HPLC-MS/MS, SDS-PAGE, and colorimetry showed that, during the conversion of FXIII to FXIIIa, the vulnerability of FXIII to hypochlorite-induced oxidation increased. FXIII oxidized with
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Novel pH-Sensitive Microbial Rhodopsin from Sphingomonas paucimobilis Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-12-25 N. Maliar, I. S. Okhrimenko, L. E. Petrovskaya, A. A. Alekseev, K. V. Kovalev, D. V. Soloviov, P. A. Popov, T. I. Rokitskaya, Y. N. Antonenko, D. V. Zabelskii, D. A. Dolgikh, M. P. Kirpichnikov, V. I. Gordeliy
Abstract This work provides the first characteristics of the rhodopsin SpaR from Sphingomonas paucimobilis, aerobic bacteria associated with opportunistic infections. The sequence analysis of SpaR has shown that this protein has unusual DTS motif which has never reported in rhodopsins from Proteobacteria. We report that SpaR operates as an outward proton pump at low pH; however, proton pumping is almost
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Effect of Silicon Dioxide Nanoparticles on Syrian Hamsters Infected by Opisthorchis felineus : 1 H MRS Study of the Brain Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-12-25 M. N. Lvova, O. B. Shevelev, V. V. Serdobintseva, D. V. Kalinin, D. A. Starostenko, E. L. Zavjalov, A. L. Krivoshapkin, P. V. Logachev, V. A. Mordvinov, D. F. Avgustinovich
Abstract In recent years, silicon dioxide nanoparticles have been widely used in medicine and the pharmaceutical industry, however, their effect on the brain has hardly been studied. We assessed the effects of long-term consumption of 5-nm amorphous silicon dioxide nanoparticles (SiO2-NPs) by Syrian hamsters infected with the trematodes Opisthorchis felineus on the hippocampus and frontal cortex. Spectroscopic
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Variability and Phylogeny of the Pepper Phytoene Synthase Paralogs PSY1 and PSY2 in Species of Various Capsicum Complexes Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-12-25 A. V. Shchennikova, E. A. Dyachenko, G. I. Efremov, M. A. Filyushin, E. Z. Kochieva
Abstract Genes homologous to PSY1 and PSY2 that encode phytoene synthase isoforms in Capsicum species C. baccatum, C. chinense, C. frutescens, C. tovarii, C. eximium, and C. chacoense were identified. High conservatism of functionally significant sites of phytoene synthases of the analyzed accessions was revealed. It was found that only PSY1-based clustering of pepper species corresponds to the traditional
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Nanosized Anti-Stokes Phosphors for Antitumor Drug Delivery and Solid Tumor Theranostics Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-10-20 R. A. Akasov, P. A. Demina, V. V. Zasedateleva, N. V. Sholina, D. A. Khochenkov, A. N. Generalova, J. Senthil Selvan, E. V. Khaydukov, V. Ya. Panchenko
Abstract—Theranostics is the direction in modern biomedicine aimed at developing drugs that combine the capabilities of diagnosis and therapy of tumors in one agent. Upconversion nanophosphors (UCNPs) are inorganic crystalline materials that can be used to create a nanoplatform providing diagnostic and therapeutic modalities. They have been proposed as luminescent markers for optical imaging of biological
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Intracellular Transport of Ribosome-Inactivating Proteins Depends on Annexin 13 Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-10-20 D. V. Maltseva, M. P. Raigorodskaya, V. G. Zgoda, E. A. Tonevitsky, E. N. Knyazev
Abstract In the present study, we assessed the role of annexin 13 membrane-binding protein (ANXA13) in the intracellular transport of vesicles containing type II ribosome-inactivating proteins (RIP-IIs). A modified human intestinal epithelial cell line HT29 was used, in which the expression of ANXA13 was significantly reduced. The cytotoxic effect of ricin and viscumin was evaluated by modification
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Salicylate-Induced Chitinases in Pea Roots Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-10-20 A. M. Egorova, N. Wielsch, I. A. Tarchevsky
Abstract Three proteins induced by salicylic acid were revealed in pea roots. These proteins were identified as chitinase isozymes belonging to the glycoside hydrolases family 18. The PsCam050724 transcript encoding at least one of these isoforms was found, allowing us to determine its primary structure, which lacks the signal peptide.
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The Effect of Low and Medium Doses of Proton Pencil Scanning Beam on the Blood-Forming Organs during Total Irradiation of Mice Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-10-20 V. E. Balakin, O. M. Rozanova, E. N. Smirnova, T. A. Belyakova, A. E. Shemyakov, S. I. Zaichkina, S. S. Sorokina, A. R. Dyukina
Abstract The aim of this work was to study the effect of proton pencil beam scanning in the Bragg peak in the dose range of 0.1–1.5 Gy on the induction of cytogenetic damage in the bone marrow, reactive oxygen species (ROS) production in whole blood, and the state of lymphoid organs after total body irradiation of mice. Irradiation was carried out in the Prometeus proton synchrotron (Protvino) in the
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Expression of Protein Kinases RIPK-1 and RIPK-3 in Mouse and Human Hair Follicle Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-10-20 E. I. Morgun, E. D. Pozdniakova, E. A. Vorotelyak
Abstract Expression of cell death regulators RIPK-1 and RIPK-3 in mouse and human hair follicle structures was studied by immunohistochemistry. At anagen and catagen stages of mouse hair follicle, RIPK-1+ cells were located in the inner root sheath, whereas RIPK-3+ cells were found in the inner and outer root sheath, dermal papilla, and interfollicular epidermis. RIPK-1 expression intensity was low
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Dipeptide Mimetics of Different NGF and BDNF Loops Activate PLC-γ1 Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-10-20 T. A. Gudasheva, I. O. Logvinov, S. V. Nikolaev, T. A. Antipova, P. Yu. Povarnina, S. B. Seredenin
Abstract Previously, we designed and synthesized dipeptide mimetics of individual loops of the nerve growth factor (NGF) and the brain-derived neurotrophic factor (BDNF). It was shown that these mimetics activate the corresponding tyrosine kinase (Trk) receptors and have different patterns of activation of the PI3K/AKT and MAPK/ERK postreceptor signaling pathways in vitro. In the present study, it
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Expressions of Pathogenesis related 1 ( PR1 ) Gene in Solanum lycopersicum and Influence of Salicylic Acid Exposures on Host- Meloidogyne incognita Interactions Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-10-20 Refik Bozbuga
Abstract Salicylic acid (SA) induces the mechanism of the plant defence and plays a role in plant -pathogen interactions. Following the nematode infection, effects of SA treatment on plant weights and the expression of pathogen related gene have not been fully understood. The present study was aimed to determine the effects of SA treatment on the expression Pathogenesis related 1 gene (PR1 gene) and
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Modulation of Calcium-Activated Chloride Currents in Rat Neurons with New 2-Aminotiophene-3-Carboxylic Acid Derivatives Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-10-20 V. L. Zamoyski, V. V. Grigoriev, A. Yu. Aksinenko, S. O. Bachurin
Abstract Using the patch-clamp method in the whole cell configuration, it was shown that new conjugates of 2-aminothiophene-3-carboxylic acid with adamantane derivatives exhibit the ability to modulate CaCC activity in the single Purkinje neurons of rat cerebellum. It was noted that, depending on the nature of the substitution in the thiophene fragment, the nature of the effect on CaCC varies from
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Indolyl- and Pyrrolylazine Derivatives Cause the Accumulation of Heat Shock Protein Hsp70 in Sh-Sy5Y Human Neuroblastoma Cells Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-10-20 V. F. Lazarev, E. R. Mikhaylova, M. A. Mikeladze, M. A. Trestsova, I. A. Utepova, O. N. Chupakhin, B. A. Margulis, I. V. Guzhova
Abstract The heat shock protein Hsp70 is involved in cell defense from various types of stress, including the proteotoxic stress, which occurs during the development of many neurodegenerative diseases. This work presents data on the detection of small molecules, derivatives of indolyl- and pyrrolylazines, which can activate the synthesis of Hsp70 and cause its accumulation in the cell. The toxicity
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Cognitive Enhancer Noopept Activates Transcription Factor HIF-1 Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-10-20 L. F. Zainullina, T. V. Ivanova, S. V. Sadovnikov, Yu. V. Vakhitova, S. B. Seredenin
Abstract The effect of noopept (N-phenylacetyl-L-prolyl-glycine ethyl ester) on the DNA-binding activity of HIF-1 in SH-SH5Y cells and the mechanisms of stabilization of this transcription factor were studied in vitro. Noopept was shown to increase both the basal DNA-binding activity of HIF-1 and the activity induced by various hypoxia mimetics. The mechanism of stabilization of the oxygen-sensitive
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A Higher Level of Expression of the Nucleolar Protein SURF6 in Human Normal Activated Lymphocytes and in Lymphocytes of Patients with Lymphoproliferative Disorders Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-10-20 A. A. Moraleva, M. A. Malysheva, S. V. Khajdukov, O. V. Zatsepina
Abstract Proliferation of mammalian cells is often accompanied by an increase in the content of the nucleolar proteins, which allows researchers to consider such proteins as potential activation markers. To test this assumption experimentally, we examined the expression pattern of the nucleolar rRNA processing factor SURF6 in normal (resting) peripheral blood lymphocytes, lymphocytes activated for
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Cold Stress Activates the Expression of Genes of the Chloroplast Transcription Apparatus in Arabidopsis thaliana Plants Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-10-20 I. A. Bychkov, N. V. Kudryakova, Vl. V. Kuznetsov, V. V. Kusnetsov
Abstract The physiological and molecular responses of Arabidopsis thaliana plants to cold stress were studied. Exposure to a low non-freezing temperature (4°C, 5 days) caused a decrease in the physiological functions and activity of a number of photosynthetic genes and elevation in expression of the cold stress gene COR15a, the product of which protects chloroplasts. It was shown for the first time
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Relationship between the Expression Level of PSMD11 and Other Proteasome Proteins with the Activity of Ricin and Viscumin. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-09-07 D V Maltseva,M P Raigorodskaya,O V Tikhonova,E N Knyazev,E A Tonevitsky
Abstract –The role of proteasome proteins and proteins of the ERAD system in the cytotoxicity of type II ribosome-inactivating proteins ricin and viscumin was investigated. For this, the cell line of colorectal adenocarcinoma HT29, as well as the HT29-sh002 line obtained on its basis, were used. On the basis on the proteome analysis of these lines and the estimation of the proportion of inactivated
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Comparative Analysis of Long Noncoding RNA Expression in Human Hepatocyte Cell Lines and Liver. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-09-07 O Y Burenina,T S Zatsepin,E F Kim,A V Metelin,D A Skvortsov,M P Rubtsova,O A Dontsova
AbstractLong noncoding RNAs (lncRNAs) are promising biomarkers and potential targets for liver cancer therapy. Stable hepatocyte lines are used in vitro to investigate functions of lncRNAs which amount in cell fluctuates during carcinogenesis. For the first time we compared gene expression of known lncRNAs in human conditional normal liver cells HepaRG and cancer cell lines Huh7 and HepG2. We showed
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Differences in the Drosha and Dicer Cleavage Profiles in Colorectal Cancer and Normal Colon Tissue Samples. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-09-07 M Yu Shkurnikov,S A Nersisyan,A Sh Osepyan,D V Maltseva,E N Knyazev
AbstractHuman colorectal adenocarcinoma cell line Caco-2 is often used as a model of healthy intestinal epithelium, in particular, in miRNA studies. The work of the enzymes Drosha and Dicer is an integral part of the process of miRNA formation. Inaccuracies in the work of these enzymes lead to a change in the nucleotide sequences of miRNAs with the formation of new isoforms, which, in turn, can change
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Use of Genetically Encoded Fluorescent Aptamers for Visualization of Mycobacterium tuberculosis Small RNA MTS1338 in Infected Macrophages. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-09-07 O S Bychenko,Yu V Skvortsova,A S Grigorov,T L Azhikina
AbstractThe possibility to visualize small bacterial RNAs inside macrophages infected with Mycobacterium tuberculosis was demonstrated for the first time. A macrophage cell line was infected with the M. tuberculosis strain expressing small noncoding mycobacterial RNA MTS1338 fused with an RNA aptamer, which could bind a fluorophore and trigger its fluorescence. As a result, treatment of the infected
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The Influence of the Cholesterol Level in Cells on Endovanilloid Cytotoxicity. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-09-07 M G Akimov,P V Dudina,A M Gamisonia,N M Gretskaya,G N Zinchenko,C C Mandal,V V Bezuglov
AbstractThe influence of the cellular cholesterol content on the cytotoxicity of endovanilloids acyldopamines was studied in MDA-MB-231 and MCF 10A cells. The activity of acyldopamines depends on the cellular cholesterol content, and a decrease in cholesterol content increases the cytotoxicity of acyldopamines.
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Recombinant Analogue of the Human Protein SLURP-1 Inhibits the Growth of U251 MG and A172 Glioma Cells. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-09-07 M A Shulepko,M L Bychkov,E N Lyukmanova,M P Kirpichnikov
AbstractThe alpha7 nicotinic acetylcholine receptor (α7-nAChR) is considered a promising pharmacological target for the carcinoma therapy. We have previously shown that the recombinant analogue of the human protein SLURP-1 (rSLURP-1) effectively inhibits the growth of carcinomas of various origins via the interaction with α7-nAChR and down-regulation of expression of this receptor. Expression of α7-nAChR
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Peptide Blocker of Ion Channel TRPV1 Exhibits a Long Analgesic Effect in the Heat Stimulation Model. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-09-07 O V Sintsova,V A Palikov,Y A Palikova,A A Klimovich,I N Gladkikh,Y A Andreev,M M Monastyrnaya,E P Kozlovskaya,I A Dyachenko,S A Kozlov,E V Leychenko
AbstractThe ion channel TRPV1, which is one of the most important integrators of pain and inflammatory stimuli, is considered a promising therapeutic target in the treatment of pain conditions. In this work, we performed a comparative study of the analgesic effect in the “hot plate” test of recombinant analogues of Kunitz-type peptides from the sea anemone Heteractis crispa venom: APHC1—modulator of
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The Anxiolytic Effect of the Neuropeptide Cycloprolylglycine Is Mediated by AMPA and TrkB Receptors. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-09-07 T A Gudasheva,P Yu Povarnina,K N Koliasnikova,A G Alyaeva,O N Vorontsova,S B Seredenin
AbstractPreviously we have shown that the neuropeptide cycloprolylglycine is an endogenous positive modulator of AMPA receptors and assumed that the pharmacological effects of CPG are associated with the brain neurotrophic factor. In this paper, we have first demonstrated that DNQX, an inhibitor of AMPA receptors, and K252A, an ihibitor of Trk receptors, prevented the anxiolytic effect of CPG, which
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The Role of the Brain in the Regulation of Peripheral Organs-Noradrenaline Sources in Neonatal Rats: Noradrenaline Synthesis Enzyme Activity. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-09-07 A R Murtazina,Yu O Nikishina,M V Ugrumov
AbstractThis work is aimed at studying the mechanisms of reciprocal humoral regulation of noradrenaline-producing organs in rats in the perinatal period of development. The activity of noradrenaline synthesis enzymes tyrosine hydroxylase and dopamine-beta-hydroxylase was measured in the brain and adrenal glands 48 and 72 h after the injection of immunotoxin (anti-dopamine-beta-hydroxylase-saporin)
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Influence of Internal and External Factors on the Production of Cytokines by Peripheral Blood Cells in Breast Cancer. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-09-07 A I Autenshlyus,K I Davletova,E S Mikhaylova,A V Proskura,N A Varaksin,A P Bogachuk,S V Sidorov,V V Lyakhovich,V M Lipkin
AbstractThe article focuses on the influence of human leukemia differentiation factor (HLDF), carcinoembryonic antigen (CEA), and polyclonal activators (PA) on cytokine production by peripheral blood cells in breast cancer and benign breast diseases. It was found that the influence of internal factors on the production of cytokines by the peripheral blood cells is associated with lymphatic metastasis
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Factors Involved in miRNA Processing Change Its Expression Level during Imitation of Hypoxia in BeWo b30 Cells. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-09-07 S A Nersisyan,M Yu Shkurnikov,E N Knyazev
AbstractOne of the main complications of pregnancy and causes of maternal and perinatal mortality is preeclampsia. The pathophysiology of preeclampsia is associated with the development of placenta and fetal hypoxia and secretion of a number of effective molecules. The human choriocarcinoma cell line BeWo b30 is often used as a model of the placental barrier. It was shown that oxyquinoline derivatives
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The Center of Origin and Colonization Routes of Noble Salmons of the Genus Salmo (Salmonidae, Actinopterigii). Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-09-07 V S Artamonova,S A Afanasyev,N V Bardukov,V M Golod,S V Kokodiy,A V Koulish,A N Pashkov,S K Pipoyan,S I Reshetnikov,A A Makhrov
AbstractGenetic diversity and colonization routes of noble salmons were studied using a partial nucleotide sequence of the mitochondrial COI gene. The brown trout S. trutta, which is the most ancient species of the genus, was concluded to originate from the modern southeastern Pontic-Caspian area, which is currently inhabited by members of the subspecies S. trutta oxianus. Migrating westward while
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Topological Features of Histone H2A Monoubiquitination. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-09-07 A A Kudriaeva,V M Lipkin,A A Belogurov
AbstractThe cellular response to DNA damage protects the essential information stored in the genome. This mechanism is crucial in terms of the cancer prevention and aging progression. The DNA damage response (DDR) consists of a complex network controlling the cell cycle and multiple mechanisms of the DNA repair. The DDR disruption is a cornerstone feature of the tumor cells, which allows them to enhance
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Influence of Acrylamide on Energy Status and Survival of Bacteria of Different Systematic Groups. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-07-06 Yu G Maksimova,E M Mochalova,V A Demakov
AbstractWe studied the effect of acrylamide on the content of intracellular ATP in the cells of bacteria of the genera Rhodococcus and Alcaligenes, the luminescence of the genetically engineered strain Escherichia coli K12 TG1 (pXen7), and the survival of bacteria of various systematic groups. According to the level of decrease in the concentration of intracellular ATP, it was found that the strain
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Viscous Media Slow Down the Decay of the Key Intermediate in Bacterial Bioluminescent Reaction. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-07-06 A E Lisitsa,L A Sukovatyi,V A Kratasyuk,E V Nemtseva
AbstractThe effects of medium viscosity on the decay rate of the 4a-hydroperoxyflavin intermediate of the bioluminescent reaction was investigated. It was found that at low concentrations of glycerol or sucrose (viscosity 1.1–1.3 cP) the decay rate rises, whereas a further increase in viscosity to 6.2 cP leads to a decrease in the decay rate following a power function with an exponent of 0.82–0.84
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Fluorescence Excitation Spectra of Total Lipids with Paramagnetic Properties Isolated from Marine Organisms. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-07-06 Yu A Uss,B N Krasheninnikov,V T Minligareev
AbstractResults of analysis of fluorescence excitation spectra of total lipid extracts of marine hydrobionts are presented. Previously, identical paramagnetic centers were recorded in these extracts by EPR spectroscopy. Analysis of fluorescence excitation spectra of total lipid extracts of Mytilus galloprovincialis L. (gonads) and Squalus achantias (liver) showed that, after oxidation with lead dioxide
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Antagonists D1 and D2 of Dopamine Receptors Determine Different Mechanisms of Neuroprotective Action in the Frog Vestibular. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-07-06 I V Ryzhova,T V Tobias,A D Nozdrachev
AbstractThe high functional plasticity of the glutamatergic synapse of the vestibular epithelium is supported by a delicate balance of excitatory and inhibitory interactions. One of the peptides co-localized with acetylcholine (ACh) in the efferent fibers is dopamine. Using external perfusion of the synaptic zone and multiunit recording of an afferent fibers activity the effect of dopamine antagonists
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Fatty Acid Composition of Yakut Horse Tissues. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-07-06 K A Petrov,O N Makhutova,M I Gladyshev
AbstractWe compared the composition and content of fatty acids (FAs) in the liver, muscles, and subcutaneous fat of Yakut horses inhabiting extreme environment in the Cryolithozone. Essential linoleic and alpha-linolenic acids, supplied to horses with their food, were accumulated in different tissues. Linoleic acid was accumulated in the liver but alpha-linolenic acid was accumulated in muscle and
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Kinetics of Chemical Processes in the Human Brain. The Cholinergic Synapse-Mechanisms of Functioning and Control Methods. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-07-06 S D Varfolomeev,V I Bykov,S B Tsybenova
AbstractIn the framework of the kinetic model, the functioning of the cholinergic synapse is considered. The results of mathematical modeling of changes in the level of acetylcholine, induced pH impulse, the influence of the frequency of impulse transmission and inhibition of acetylcholinesterase are presented. Physicochemical explanation for a number of important physiological phenomena, such as neuromuscular
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Deep Physical-Chemical Purification of Gas Medium in Artificial Ecosystems. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-07-06 S V Trifonov,V V Velichko,N A Tikhomirova,V N Shikhov,E A Morozov,A A Tikhomirov
AbstractThe results of experiments on application of a newly developed facility for oxidation of volatile organic compounds on a platinum catalyst are presented. The feasibility of using this method in artificial ecosystems as a whole and in mass exchange of closed biological-technical life support systems in particular is shown. The possibility of deep purification of gas emitted from the reactor
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The Structural-Functional Damage of Fibrinogen Oxidized by Hydrogen Peroxide. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-07-06 L V Yurina,A D Vasilyeva,V L Kononenko,A E Bugrova,M I Indeykina,A S Kononikhin,E N Nikolaev,M A Rosenfeld
AbstractThe effect of peroxide-induced oxidation of fibrinogen on modification of its primary structure and functional properties was investigated. The oxidation sites were shown to be Met, Trp, and His residues. Using the DLS method, it was found that the oxidative modification of fibrinogen results in the change of microrheological characteristics of fibrin network. The fibrinogen oxidation diminishes
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Control of the Rate of Fibroblast Migration and Spreading by Defensin Oligopeptide Fragment. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-07-06 V P Ivanova,A I Krivchenko
AbstractThe elaboration of modes for directed regulation of cell transition from the nonmotile fixed status to the motile state, and vice versa, is referred to the most important problems of practical medicine. Nowadays, the creation of biopreparations on the basis of naturally occurring compounds for the minimization or elimination of negative consequences at the cell malignization is an actual problem
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The Effect of Surgical Trauma in the Nasal Cavity on the Behavior in the Open Field and the Autonomic Nervous System of Rats. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-07-06 I V Kastyro,I V Reshetov,G V Khamidulin,P E Shmaevsky,O V Karpukhina,A N Inozemtsev,V I Torshin,N V Ermakova,V I Popadyuk
AbstractIn this article, the effect of surgical damage to the nasal mucosa in rats under the action of general anesthesia was investigated. The motor activity and heart rate variability (HRV) of rats were studied before and 2 days after the surgery. After surgical exposure, motor activity decreased, the time of fading and grooming increased, VLF increased, and HF decreased. Surgical trauma of the nasal
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Epitope Analysis of Murine and Chimeric Monoclonal Antibodies Recognizing the Cancer Testis Antigen PRAME. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-07-06 V A Misyurin,Yu P Finashutina,A A Turba,M V Larina,O N Solopova,N A Lyzhko,L A Kesaeva,N N Kasatkina,T K Aliev,A V Misyurin,M P Kirpichnikov
AbstractWe investigated the epitope specificity of different monoclonal antibodies recognizing the cancer testis antigen PRAME. Antibody 5D3 binds to the fragment of PRAME corresponding to 160–180 amino acid residues. Antibodies 6H8 and F11 bind to the fragment corresponding to 180–200 amino acid residues of PRAME. These antibodies retained the ability to recognize these PRAME fragments after chimerization
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The Transcription Factor HY5 Is Involved in the Cytokinin-Dependent Regulation of the Expression of Genes Encoding Proteins Associated with Bacterial Plastid RNA-Polymerase during De-Etiolation of Arabidopsis thaliana. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-07-06 A S Doroshenko,M N Danilova,A A Andreeva,N V Kudryakova,Vl V Kuznetsov,V V Kusnetsov
AbstractHY5 (ELONGATED HYPOCOTYL5), a bZIP transcription factor, is one of the main regulators of light and hormonal signaling. Among the targets of this gene, the genes for the transcriptional complex of chloroplasts whose coordinated expression ensures the initial stages of photomorphogenesis are particularly significant. In this study, we showed that, during de-etiolation, HY5 functions as a positive
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Morphological and Molecular-Biological Features of Inflammatory and Regeneratory Processes in Peridont Tissues with Periimplantitis and Periodontitis. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-07-06 A A Kulakov,E A Kogan,T V Brailovskaya,A P Vedyaeva,N V Zharkov
AbstractA morphological and immunohistochemical study of periodontal tissues was performed in patients with chronic generalized periodontitis who underwent dental implantation. We studied 44 biopsy specimens from 21 patients (13 women and 8 men) aged 35–60 years with a diagnosis of periimplantation mucositis (7 patients), periimplantitis (8 patients), and severe chronic generalized periodontitis (6
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Structural and Functional Diversification of SEPALLATA Genes TM5 and RIN in Tomato Species (Section Lycopersicon). Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-07-06 M A Slugina,E A Dyachenko,E Z Kochieva,A V Shchennikova
AbstractNew TOMATO MADS 5 (TM5) homologous genes were identified in evolutionarily recent, red-fruited and more ancient, wild green-fruited tomato species. It was shown that the identified TM5 homologs belong to the SEPALLATA3 clade; thus, the SEP subfamily diversification was characterized. For the first time, the TM5 and RIN co-expression pattern was determined in flowers, immature green fruits,
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Blockers of Nicotinic Acetylcholine Receptors Delay Tumor Growth and Increase Antitumor Activity of Mouse Splenocytes. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-06-01 T I Terpinskaya,A V Osipov,T V Balashevich,T L Yanchanka,E A Tamashionik,V I Tsetlin,Yu N Utkin
AbstractBlockade of α6, α3β2, α9α10, and α7 subtypes of nicotinic acetylcholine receptors slows tumor growth in vivo, increases cytotoxic activity of splenocytes from tumor-bearing mice, and, to some extent, reduces the viability of Ehrlich carcinoma cells in vitro. These data indicate that nicotinic acetylcholine receptors are involved in oncogenesis, affecting the survival of tumor cells, inter alia
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A New Type of Light-Harvesting Complex Detected when Growing Rhodopseudomonas palustris under Low Light Intensity Conditions. Dokl. Biochem. Biophys. (IF 0.672) Pub Date : 2020-06-01 O P Serdyuk,L D Smolygina,A A Ashikhmin
AbstractThe predominance of the maximum at 800 nm for the light-harvesting complex LH4 (B800) and at 850 nm for LH2 (B800-850) from Rps. palustris is determined by the composition of αβ-polypeptides and pigments. In low light (LL) for Rps. palustris, strain KM 286 (1e5), along with LH4, the LL LH2 complex was synthesized with the same absorption at 800 and 850 nm. It differed from the LH4 and LH2 complex