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Structural and biochemical characterization of active sites mutant in human inorganic pyrophosphatase BBA Gen. Subj. (IF 3.0) Pub Date : 2024-02-28 Shuping Zheng, Chenhua Zheng, Sishi Chen, Jianpeng Guo, Lirui Huang, Zhenhong Huang, Sunting Xu, Yihan Wu, Shunfa Li, Junjin Lin, Yiqing You, Fen Hu
Inorganic pyrophosphatases (PPases) are enzymes that catalyze the conversion of inorganic pyrophosphate (PPi) into phosphate (Pi). Human inorganic pyrophosphatase 1 (Hu-PPase) exhibits high expression levels in a variety of tumors and plays roles in cell proliferation, apoptosis, invasion and metastasis, making it a promising prognostic biomarker and a target for cancer therapy. Despite its widespread
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Insights into flowering mechanisms in apple (Malus × domestica Borkh.) amidst climate change: An exploration of genetic and epigenetic factors BBA Gen. Subj. (IF 3.0) Pub Date : 2024-02-24 Anshul Kumar, Muntazir Mushtaq, Pankaj Kumar, Dharam Paul Sharma, Vijay Gahlaut
Apple ( Borkh.) holds a prominent position among global temperate fruit crops, with flowering playing a crucial role in both production and breeding. This review delves into the intricate mechanisms governing apple flowering amidst the backdrop of climate change, acknowledging the profound influence of external and internal factors on biennial bearing, flower bud quality, and ultimately, fruit quality
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Alpha-fetoprotein upregulates hepatocellular carcinoma cell-intrinsic PD-1 expression through the LATS2/YAP/TEAD1 pathway BBA Gen. Subj. (IF 3.0) Pub Date : 2024-02-21 Guangxian Leng, Hongxia Gong, Guiyuan Liu, Yin Kong, Liuqing Guo, Youcheng Zhang
Hepatocellular carcinoma (HCC) cell-intrinsic programmed death 1 (PD-1) promotes tumor progression. However, the mechanisms that regulate its expression are unclear. This study investigated the impact of alpha-fetoprotein (AFP) on HCC cell-intrinsic PD-1 expression. The expression of PD-1 and AFP at the gene and protein levels was detected using real-time fluorescence quantitative polymerase chain
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A potent candicidal peptide designed based on an encrypted peptide from a proteinase inhibitor BBA Gen. Subj. (IF 3.0) Pub Date : 2024-02-13 Luís Henrique de Oliveira Almeida, Suellen Rodrigues Ramalho, Claudiane Vilharroel Almeida, Camila de Oliveira Gutierrez, Janaína de Cassia Orlandi Sardi, Antonio de Miranda, Ricardo Abreu de Oliveira, Samilla Beatriz de Rezende, Edson Crusca, Octávio Luiz Franco, Caio Fernando Ramalho de Oliveira, Marlon Henrique Cardoso, Maria Lígia Rodrigues Macedo
Antimicrobial peptides (AMP) represent an alternative in the treatment of fungal infections associated with countless deaths. Here, we report a new AMP, named KWI-19, which was designed based on a peptide encrypted in the sequence of an Kunitz-type inhibitor (ILTI). KWI-19 inhibited the growth of species and acted as a fungicidal agent from 2.5 to 20 μmol L, also showing synergistic activity with amphotericin
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Evaluation of molecular mechanisms of riboflavin anti-COVID-19 action reveals anti-inflammatory efficacy rather than antiviral activity BBA Gen. Subj. (IF 3.0) Pub Date : 2024-02-08 Roman A. Akasov, Olga E. Chepikova, Tatiana N. Pallaeva, Neonila V. Gorokhovets, Andrei E. Siniavin, Vladimir A. Gushchin, Lyudmila V. Savvateeva, Ivan A. Vinokurov, Dmitry A. Khochenkov, Andrey A. Zamyatnin, Evgeny V. Khaydukov
Riboflavin (vitamin B2) is one of the most important water-soluble vitamins and a coenzyme involved in many biochemical processes. It has previously been shown that adjuvant therapy with flavin mononucleotide (a water-soluble form of riboflavin) correlates with normalization of clinically relevant immune markers in patients with COVID-19, but the mechanism of this effect remains unclear. Here, the
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Methoxychalcones as potential anticancer agents for colon cancer: Is membrane perturbing potency relevant? BBA Gen. Subj. (IF 3.0) Pub Date : 2024-02-08 Anna Palko-Łabuz, Olga Wesołowska, Maria Błaszczyk, Anna Uryga, Beata Sobieszczańska, Magdalena Skonieczna, Edyta Kostrzewa-Susłow, Tomasz Janeczko, Kamila Środa-Pomianek
Chalcones are naturally produced by many plants, and constitute precursors for the synthesis of flavons and flavanons. They were shown to possess antibacterial, antifungal, anti-cancer, and anti- inflammatory properties. The goal of the study was to assess the suitability of three synthetic methoxychalcones as potential anticancer agents. In a panel of colon cancer cell lines they were demonstrated
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Epigenetic regulation influenced by soil microbiota and nutrients: Paving road to epigenome editing in plants BBA Gen. Subj. (IF 3.0) Pub Date : 2024-02-05 Bhavya Doddavarapu, Charu Lata, Jasmine M. Shah
Soil is a complex ecosystem that houses microbes and nutrients that are necessary for plant development. Edaphic properties of the soil and environmental conditions influence microbial growth and nutrient accessibility. Various environmental stimuli largely affect the soil microbes and ionic balance, in turn influencing plants. Soil microflora helps decompose organic matter and is involved in mineral
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Understanding the structural and functional changes and biochemical pathomechanism of the cardiomyopathy-associated p.R123W mutation in human αB-crystallin BBA Gen. Subj. (IF 3.0) Pub Date : 2024-02-01 Leila Rezaei Somee, Anis Barati, Mohammad Bagher Shahsavani, Masaru Hoshino, Jun Hong, Ashutosh Kumar, Ali Akbar Moosavi-Movahedi, Massoud Amanlou, Reza Yousefi
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FBXO8 is a novel prognostic biomarker in different molecular subtypes of breast cancer and suppresses breast cancer progression by targeting c-MYC BBA Gen. Subj. (IF 3.0) Pub Date : 2024-01-30 Abdul Jamil Khan, Shad Man, Manzar Abbas, Shihao Liu, Feng Zhang
F-box only protein 8 (FBXO8) is a recently identified member of the F-box proteins, showcasing its novelty in this protein family. Extensive research has established FBXO8's role as a tumor suppressor in various cancers, including hepatocellular carcinoma, and colorectal cancer, Nevertheless, its functional, mechanistic, and prognostic roles in primary and metastatic breast cancer, particularly in
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Selenomethionine modulates the JAK2 / STAT3 / A20 pathway through oxidative stress to alleviate LPS-induced pyroptosis and inflammation in chicken hearts BBA Gen. Subj. (IF 3.0) Pub Date : 2024-01-23 Yutian Lei, Wenying Sun, Tong Xu, Jianhua Shan, Meichen Gao, Hongjin Lin
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Charge manipulation of the human insulin B chain C-terminal to shed light on the complex mechanism of insulin fibrillation BBA Gen. Subj. (IF 3.0) Pub Date : 2024-01-24 Mohammad Bagher Shahsavani, Masaru Hoshino, Ashutosh Kumar, Reza Yousefi
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Insights into the roles of non-coding RNAs and angiogenesis in glioblastoma: An overview of current research and future perspectives BBA Gen. Subj. (IF 3.0) Pub Date : 2024-01-18 Zhengfei Song, Zhaoliang Xue, Yirong Wang, Muhammad Imran, Mohammed Assiri, Shah Fahad
Glioblastoma (GBM) is a highly aggressive type of primary brain cancer with a poor prognosis, and despite intensive research, survival rates have not significantly improved. Non-coding RNAs (ncRNAs) are emerging as critical regulators of GBM pathogenesis, including angiogenesis, which is essential for tumor growth and invasion. MicroRNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs)
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Alterations in the chromatin packaging, driven by transcriptional activity, revealed by AFM BBA Gen. Subj. (IF 3.0) Pub Date : 2024-01-17 V. Yu Bairamukov, R.A. Kovalev, A.V. Ankudinov, R.A. Pantina, N.D. Fedorova, A.S. Bukatin, S.V. Grigoriev, E. Yu Varfolomeeva
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Gastric cancer patient-derived organoids model for the therapeutic drug screening BBA Gen. Subj. (IF 3.0) Pub Date : 2024-01-19 Jiao Xu, Jin Gong, Mengyang Li, Ye Kang, Jinrong Ma, Xi Wang, Xiao Liang, Xin Qi, Bixin Yu, Jin Yang
Background Gastric cancer (GC) is a highly heterogeneous disease featuring many various histological and molecular subtypes. Therefore, it is imperative to have well-characterized in vitro models for personalized treatment development. Gastric cancer patient-derived organoids (PDOs), re-capitulating in vivo conditions, exhibit high clinical efficacy in predicting drug sensitivity to facilitate the
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N-glycan on N262 of FGFR3 regulates the intracellular localization and phosphorylation of the receptor BBA Gen. Subj. (IF 3.0) Pub Date : 2024-01-18 Ukichiro Hashimoto, Naoki Fujitani, Yasuaki Uehara, Hiromi Okamoto, Atsushi Saitou, Fumie Ito, Shigeru Ariki, Akiko Shiratsuchi, Yoshihiro Hasegawa, Motoko Takahashi
N-glycosylation and proper processing of N-glycans are required for the function of membrane proteins including cell surface receptors. Fibroblast growth factor receptor (FGFR) is involved in a wide variety of biological processes including embryonic development, osteogenesis, angiogenesis, and cell proliferation. Human FGFR3 contains six potential N-glycosylation sites, however, the roles of glycosylation
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Piperidinium surfactants functionalized with carbamate fragment: Aggregation, antimicrobial activity and cytotoxicity BBA Gen. Subj. (IF 3.0) Pub Date : 2024-01-11 Rushana A. Kushnazarova, Alla B. Mirgorodskaya, Denis M. Kuznetsov, Elmira A. Vasilieva, Syumbelya K. Amerhanova, Alexandra D. Voloshina, Lucia Ya. Zakharova
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Distinctive domains and activity regulation of core fucosylation enzyme FUT8 BBA Gen. Subj. (IF 3.0) Pub Date : 2024-01-12 Seita Tomida, Masamichi Nagae, Yasuhiko Kizuka
Background Core fucose, a structure added to the reducing end N-acetylglucosamine of N-glycans, has been shown to regulate various physiological and pathological processes, including melanoma metastasis, exacerbation of chronic obstructive pulmonary disease, and severe outcomes in COVID-19. Scope of review Recent research has shed light on regulation of the activity and subcellular localization of
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The 60 nm gold nanoparticles improve qPCR amplification efficiency through specific palindromic sequences (GGATCC or ACCGGT) in primers BBA Gen. Subj. (IF 3.0) Pub Date : 2024-01-09 Ruyu Zeng, Zhiqun Du, Hongliang Ma, Xiuqiong Meng, Erhua Li, Jiangchao Li
Background Polymerase chain reaction (PCR) technology and quantitative real-time PCR (qPCR) technology are widely used in clinical diagnosis and research, but amplification efficiency and sensitivity are still key problems for researchers. An increasing number of reports show that gold nanoparticles (AuNPs) can be used to improve the sensitivity and amplification efficiency of PCR. Here, we found that
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Nanotechnology in the diagnostic and therapy for Alzheimer's disease BBA Gen. Subj. (IF 3.0) Pub Date : 2024-01-06 Archna Panghal, S.J.S. Flora
Alzheimer's disease (AD) is a neurodegenerative disorder primarily characterized by β-amyloid plaque, intraneuronal tangles, significant neuronal loss and cognitive deficit. Treatment in the early stages of the disease is crucial for preventing or perhaps reversing the neurodegeneration in the AD cases. However, none of the current diagnostic procedures are capable of early diagnosis of AD. Further
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Advanced nanoscale delivery systems for mRNA-based vaccines BBA Gen. Subj. (IF 3.0) Pub Date : 2024-01-05 Maha Mobasher, Rais Ansari, Ana M. Castejon, Jaleh Barar, Yadollah Omidi
The effectiveness of messenger RNA (mRNA) vaccines, especially those designed for COVID-19, relies heavily on sophisticated delivery systems that ensure efficient delivery of mRNA to target cells. A variety of nanoscale vaccine delivery systems (VDSs) have been explored for this purpose, including lipid nanoparticles (LNPs), liposomes, and polymeric nanoparticles made from biocompatible polymers such
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HERC4 modulates ovarian cancer cell proliferation by regulating SMO-elicited hedgehog signaling BBA Gen. Subj. (IF 3.0) Pub Date : 2024-01-03 Qingjuan Zhu, Xin Yang, Yuchun Lv
Background HERC4 has been reported to have functions in several types of tumors, but its roles in ovarian cancer have not been studied yet. Methods Primary tissues from ovarian cancer patients and cell lines were collected for real-time PCR. Kaplan-Meier Plotter was used to predict the prognosis of ovarian cancer patients. HERC4 was overexpressed in cells by lentivirus, and CCK-8 assay was performed
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Roxadustat ameliorates experimental colitis in mice by regulating macrophage polarization through increasing HIF level BBA Gen. Subj. (IF 3.0) Pub Date : 2023-12-27 Guiping Kong, Hu Hua, Yan Lu, Kunlong Yan, Yucan Zheng, Zhanjun Jia, Hongmei Guo, Mei Li, Yu Jin, Zhifeng Liu
Background Gastro-intestinal (GI) tract inflammation is as a result of inflammatory hypoxia which is also induced by long-standing group of disorders like inflammatory-bowel disease (IBD). Regulation of GI immune homeostasis by macrophage involves hypoxia-inducible factor (HIF). As inhibitor of HIF prolyl hydroxylase, roxadustat (ROX) increases the levels of HIF. Methods We induced experimental colitis
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Crystal structure of the GH-46 subclass III chitosanase from Bacillus circulans MH-K1 in complex with chitotetraose BBA Gen. Subj. (IF 3.0) Pub Date : 2023-12-27 Michihiko Suzuki, Akihiro Saito, Mariko Kobayashi, Tomofumi Yokoyama, Shoko Omiya, Jian Li, Kei Sugita, Kunio Miki, Jun-ichi Saito, Akikazu Ando
Background Chitosanases (EC 3.2.1.132) hydrolyze chitosan which is a polymer of glucosamine (GlcN) linked by β − 1,4 bonds, and show cleavage specificity against partially acetylated chitosan containing N-acetylglucosamine (GlcNAc) residues. Chitosanases' structural underpinnings for cleavage specificity and the conformational switch from open to closed structures are still a mystery. Methods The GH-46
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Characterization of tumor-associated endothelial cells and the development of a prognostic model in pancreatic ductal adenocarcinoma BBA Gen. Subj. (IF 3.0) Pub Date : 2023-12-21 Jun Wu, Yang Liu, Qi Fu, Zhi Cao, Xiaodong Ma, Xun Li
Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive malignancy characterized by a complex tumor microenvironment. Angiogenesis is of paramount importance in the proliferation and metastasis of PDAC. However, currently, there are no well-defined biomarkers available to guide the prognosis and treatment of PDAC. In this study, we investigated the interactions between tumor-associated endothelial
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DHX33 mediates p53 to regulate mevalonate pathway gene transcription in human cancers BBA Gen. Subj. (IF 3.0) Pub Date : 2023-12-22 Guangli Nie, Shiyun Chen, Qingzhi Song, Dongxu Zou, Maggie Li, Xiyu Tang, Yuanlian Deng, Bizhou Huang, Mengxia Yang, Guoqing Lv, Yandong Zhang
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Photophysical, rotational and translational properties of Radachlorin photosensitizer upon binding to serum albumins BBA Gen. Subj. (IF 3.0) Pub Date : 2023-12-21 A.V. Belashov, A.A. Zhikhoreva, I.A. Gorbunova, M.E. Sasin, I.V. Semenova, O.S. Vasyutinskii
Introduction Although photophysical properties of Radachlorin photosensitizer (PS) were extensively studied in solutions and cells, no data is available on variations of its characteristics upon binding to serum albumins, which are major transporters in blood and nutrients in cell culture media. Objectives The primary objective of this study was to analyze changes in photophysical properties of Radachlorin
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Calcimycin mediates apoptosis in breast and cervical cancer cell lines by inducing intracellular calcium levels in a P2RX4-dependent manner BBA Gen. Subj. (IF 3.0) Pub Date : 2023-12-14 , Prashant Ranjan, Parimal Das
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Crystallographic and functional studies of a plant temperature-induced lipocalin BBA Gen. Subj. (IF 3.0) Pub Date : 2023-12-14 Chen-Song Dong, Wei-Lun Zhang, Xiao-Ying Wang, Xiao Wang, Jia Wang, Mingzhu Wang, Ying Fang, Lin Liu
Arabidopsis thaliana temperature-induced lipocalin (AtTIL) is a prototypical member of plant lipocalins and participates in a variety of cellular processes, particularly stress responses. Bioinformatical and physiological studies have proposed its promiscuous ligand-binding ability, but the molecular basis is yet unclear. Here, we report the 1.9-Å crystal structure of AtTIL in complex with heme. Spectrophotometric
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Manipulating epigenetic diversity in crop plants: Techniques, challenges and opportunities BBA Gen. Subj. (IF 3.0) Pub Date : 2023-12-16 Vikas Kumar Singh, Shoeb Ahmed, Dinesh Kumar Saini, Vijay Gahlaut, Sunena Chauhan, Kiran Khandare, Ashutosh Kumar, Pradeep Kumar Sharma, Jitendra Kumar
Epigenetic modifications act as conductors of inheritable alterations in gene expression, all while keeping the DNA sequence intact, thereby playing a pivotal role in shaping plant growth and development. This review article presents an overview of techniques employed to investigate and manipulate epigenetic diversity in crop plants, focusing on both naturally occurring and artificially induced epialleles
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METTL3/IGF2BP3-regulated m6A modification of HYOU1 confers doxorubicin resistance in breast cancer BBA Gen. Subj. (IF 3.0) Pub Date : 2023-12-15 Shaocheng Zhou, Lijuan Sheng, Lin Zhang, Jianan Zhang, Lei Wang
Chemoresistance is a main reason for therapeutic failure and poor prognosis for breast cancer (BC) patients, especially for triple-negative BC patients. How the molecular mechanisms underlying the chemoresistance to doxorubicin (Dox) in BC is not well understood. Here, we revealed that METTL3/IGF2BP3-regulated m6A modification of HYOU1 increased Dox resistance in BC cells. CCK-8 and Annexin V-FITC/PI
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Engineered extracellular vesicles expressing ICAM-1: A promising targeted delivery system for T cell modifications BBA Gen. Subj. (IF 3.0) Pub Date : 2023-12-15 Mahboube Shahrabi Farahani, Elham Hosseini-Beheshti, Seyed Mohammad Moazzeni, Mehdi Forouzandeh Moghadam
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Biosynthesis, characterization, magnetic hyperthermia, and in vitro toxicity evaluation of quercetin-loaded magnetoliposome lipid bilayer hybrid system on MCF-7 breast cancer BBA Gen. Subj. (IF 3.0) Pub Date : 2023-12-14 Shehab Elbeltagi, Ahmad M. Saeedi, Zienab E. Eldin, Haifa E. Alfassam, Hanan M. Alharbi, Nawal Madkhali, Abo Bakr Abdel Shakor, Mohamed Abd El-Aal
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Identification and prediction of molecular subtypes of atherosclerosis based on m6A immune cell infiltration BBA Gen. Subj. (IF 3.0) Pub Date : 2023-12-08 Bowen Xu, Hongye Li, Hongping Chen, Wenlong Wang, Wenjuan Jia, Lei Gong, Lin Zhong, Jun Yang
Background Atherosclerosis is a complex disease with multiple molecular subtypes that are not yet fully understood. Recent studies have suggested that N6-methyladenosine (m6A) alterations may play a role in the pathogenesis of atherosclerosis. However, the relationship between m6A regulators and atherosclerosis remains unclear. Methods In this study, we analyzed the expression levels of 25 m6A regulators
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Biomolecular dynamics in the 21st century BBA Gen. Subj. (IF 3.0) Pub Date : 2023-12-06 Charles L. Brooks, Alexander D. MacKerell, Carol B. Post, Lennart Nilsson
The relevance of motions in biological macromolecules has been clear since the early structural analyses of proteins by X-ray crystallography. Computer simulations have been applied to provide a deeper understanding of the dynamics of biological macromolecules since 1976, and are now a standard tool in many labs working on the structure and function of biomolecules. In this mini-review we highlight
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Effects of hyperglycemia on the TGF-β pathway in trabecular meshwork cells BBA Gen. Subj. (IF 3.0) Pub Date : 2023-12-10 Hsin-Yi Chen, Mei-Lan Ko, Hong-Lin Chan
Background Hyperglycemia, which can lead to apoptosis, hypertrophy, fibrosis, and induces hyperinflammation in diabetic vascular complications due to oxidative stress. In order to elucidate the potential dual roles and regulatory signal transduction of TGF-β1 and TGF-β2 in human trabecular meshwork cells (HTMCs), we established an oxidative cell model in HTMCs using 5.5, 25, 50, and 100 mM d-glucose-supplemented
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Plant histone variants at the nexus of chromatin readouts, stress and development BBA Gen. Subj. (IF 3.0) Pub Date : 2023-12-10 G. Vivek Hari Sundar, Aravind Madhu, A. Archana, P.V. Shivaprasad
Histones are crucial proteins that are involved in packaging the DNA as condensed chromatin inside the eukaryotic cell nucleus. Rather than being static packaging units, these molecules undergo drastic variations spatially and temporally to facilitate accessibility of DNA to replication, transcription as well as wide range of gene regulatory machineries. In addition, incorporation of paralogous variants
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Each N-glycan on human IgA and J-chain uniquely affects oligomericity and stability BBA Gen. Subj. (IF 3.0) Pub Date : 2023-12-08 Shunli Pan, Noriyoshi Manabe, Shiho Ohno, Sachiko Komatsu, Tsutomu Fujimura, Yoshiki Yamaguchi
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ReBaTSA: A simplified CeTSA protocol for studying recombinant mutant proteins in bacterial extracts BBA Gen. Subj. (IF 3.0) Pub Date : 2023-12-03 Maria Monticelli, Demi Marie Wright, Maria Vittoria Cubellis, Giuseppina Andreotti
Introduction The study of protein stability is crucial to biochemistry and relies on different methodologies. Recently, the Cellular Thermal Shift Assay has been introduced to study protein stability in whole cells. Methods We report a novel application of CeTSA named ReBaTSA. This Recombinant Bacterial TSA was performed using clear extracts from bacteria expressing a recombinant protein, incubated
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Formation of free acetaldehydes derived from lipid peroxidation in U937 monocyte-like cells BBA Gen. Subj. (IF 3.0) Pub Date : 2023-12-02 Pavel Pospíšil, Ankush Prasad, Julie Belková, Renuka Ramalingam Manoharan, Michaela Sedlářová
Acetaldehyde can be found in human cells as a byproduct of various metabolic pathways, including oxidative processes such as lipid peroxidation. This secondary product of lipid peroxidation plays a role in various pathological processes, leading to various types of civilization diseases. In this study, the formation of free acetaldehyde induced by oxygen-centred radicals was studied in monocyte-like
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Release of a novel peptide from ferritin nanocages: A new tool for therapeutic applications BBA Gen. Subj. (IF 3.0) Pub Date : 2023-12-02 Carolina Canè, Rosanna Lucignano, Angela Di Somma, Maria Liccardo, Clara Iannuzzi, Angela Duilio, Delia Picone
The development of new drug delivery systems for targeted chemotherapy release in cancer cells represents a very promising tool. In this contest, protein-based nanocages have considerable potential as drug delivery devices. Notably, ferritin has emerged as an excellent candidate due to its unique architecture, surface properties and high biocompatibility. A promising strategy might then involve ferritin
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Retrovirus-like gag protein Arc/Arg3.1 is involved in extracellular-vesicle-mediated mRNA transfer between glioma cells BBA Gen. Subj. (IF 3.0) Pub Date : 2023-11-22 Aya Al Othman, Dmitry Bagrov, Julian M. Rozenberg, Olga Glazova, Gleb Skryabin, Elena Tchevkina, Alexandre Mezentsev, Mikhail Durymanov
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Inorganic polyphosphate regulates functions of thymocytes via activation of P2X purinoreceptors BBA Gen. Subj. (IF 3.0) Pub Date : 2023-11-23 Kamila S. Nebesnaya, Albert R. Makhmudov, Khondamir R. Rustamov, Nigina S.H. Rakhmatullina, Sarvinoz I. Rustamova, Ulugbek Z. Mirkhodjaev, Oksana S. Charishnikova, Ravshan Z. Sabirov, Artyom Y. Baev
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High levels of FAD autofluorescence indicate pathology preceding cell death BBA Gen. Subj. (IF 3.0) Pub Date : 2023-11-11 Ekaterina O. Bryanskaya, Andrey Y. Vinokurov, Angelina I. Dolgikh, Andrey V. Dunaev, Plamena R. Angelova, Andrey Y. Abramov
Flavin adenine dinucleotide (FAD) autofluorescence from cells reports on the enzymatic activity which involves FAD as a cofactor. Most of the cellular FAD fluorescence comes from complex II of the electron transport chain in mitochondria and can be assessed with inhibitor analysis. The intensity of FAD autofluorescence is not homogeneous and vary between cells in tissue and in cell culture types. Using
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High-fat high-fructose diet and alpha-ketoglutarate affect mouse behavior that is accompanied by changes in oxidative stress response and energy metabolism in the cerebral cortex BBA Gen. Subj. (IF 3.0) Pub Date : 2023-11-13 Oleh Demianchuk, Myroslava Vatashchuk, Dmytro Gospodaryov, Viktoria Hurza, Marian Ivanochko, Vitalii Derkachov, Vladyslav Berezovskyi, Oleh Lushchak, Kenneth B. Storey, Maria Bayliak, Volodymyr I. Lushchak
Background High caloric diets with high amounts of fats and sweeteners such as fructose may predispose organisms to neurodegenerative diseases. Methods This study aimed to examine the effects of a high-fat high-fructose diet (HFFD) on the behavior of mice, energy metabolism, and markers of oxidative stress in murine cerebral cortex. Dietary α-ketoglutarate (AKG) was chosen as a treatment which could
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Structural and functional characterization of triketone dioxygenase from Oryza Sativa BBA Gen. Subj. (IF 3.0) Pub Date : 2023-11-13 Stephen M.G. Duff, Meiying Zhang, Fred Zinnel, Timothy Rydel, Christina M. Taylor, Danqi Chen, Gregory Tilton, Patricia Mamanella, David Duda, Yanfei Wang, Bosong Xiang, Balasulojini Karunanandaa, Rita Varagona, Jaishree Chittoor, Qungang Qi, Erin Hall, Graeme Garvey, Jiamin Zeng, Jun Zhang, Xin Li, Jeff Haas
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NGLY1: A fascinating, multifunctional molecule BBA Gen. Subj. (IF 3.0) Pub Date : 2023-11-09 Tadashi Suzuki, Haruhiko Fujihira
NGLY1, a cytoplasmic de-N-glycosylating enzyme is well conserved among eukaryotes. This enzyme has attracted considerable attention after mutations on the NGLY1 gene were found to cause a rare genetic disorder called NGLY1 deficiency. Recent explosive progress in NGLY1 research has revealed multi-functional aspects of this protein.
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FAT1 inhibits AML autophagy and proliferation via downregulating ATG4B expression BBA Gen. Subj. (IF 3.0) Pub Date : 2023-11-10 Nan Zhang, Meng-yu Shen, Qing-li Meng, Hao-ping Sun, Fang-yi Fan, Hai Yi, Yong-jian Yang
Background Emerging studies have shown that FAT atypical cadherin 1 (FAT1) and autophagy separately inhibits and promotes acute myeloid leukemia (AML) proliferation. However, it is unknown whether FAT1 were associated with autophagy in regulating AML proliferation. Methods AML cell lines, 6-week-old male nude mice and AML patient samples were used in this study. qPCR/Western blot and cell viability/3H-TdR
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Zinc deficiency increases lung inflammation and fibrosis in obese mice by promoting oxidative stress BBA Gen. Subj. (IF 3.0) Pub Date : 2023-11-10 Rumei Luan, Manyu Luo, Dongyan Ding, Xin Su, Junling Yang
Background Zinc deficiency can lead to multiple organ damage. In this study, we investigated the effects of zinc deficiency on obesity-related lung damage. Methods C57BL/6 J mice were fed a diet with differing amounts of zinc and fat over a 6-month period. Palmitic acid was used to stimulate A549 cells to construct a high-fat alveolar epithelial cell model. Western blotting and histopathological staining
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Role of calpain-5 in cerebral ischemia and reperfusion injury BBA Gen. Subj. (IF 3.0) Pub Date : 2023-11-09 Yusaku Chukai, Ginga Ito, Yasuo Miki, Koichi Wakabayashi, Ken Itoh, Eriko Sugano, Hiroshi Tomita, Tomokazu Fukuda, Taku Ozaki
Background Ischemia and reperfusion (I/R) injury exacerbate the prognosis of ischemic diseases. The cause of this exacerbation is partly a mitochondrial cell death pathway. Mitochondrial calpain-5 is proteolyzed/autolyzed under endoplasmic reticulum stress, resulting in inflammatory caspase-4 activation. However, the role of calpain-5 in I/R injury remains unclear. We hypothesized that calpain-5 is
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DNAJB12 and DNJB14 are non-redundant Hsp40 redox chaperones involved in endoplasmic reticulum protein reflux BBA Gen. Subj. (IF 3.0) Pub Date : 2023-11-03 Aline Dias da Purificação, Victor Debbas, Leonardo Yuji Tanaka, Gabriele Verônica de Mello Gabriel, João Wosniak Júnior, Tiphany Coralie De Bessa, Sheila Garcia-Rosa, Francisco Rafael Martins Laurindo, Percillia Victoria Santos Oliveira
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The role of proteolytic cleavage at Arg336 and Arg372 of the A1 domain in factor VIIa/tissue factor-catalyzed reactions of B domain-deleted factor VIII BBA Gen. Subj. (IF 3.0) Pub Date : 2023-11-02 Yuto Nakajima, Keiji Nogami
Background We previously demonstrated that factor (F)VIII was rapidly activated through proteolytic cleavage at Arg372 and Arg740 by activated FVII (FVIIa)/tissue factor (TF) in very early coagulation phase, followed by inactivation by cleavage at Arg336. The influence of the absence of FVIII B domain in this series of FVIIa/TF-catalyzed reaction remains unclear, however. Aim To examine the FVIIa/TF-catalyzed
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Biophysical analysis on molecular interactions between chitosan-coated sinapic acid loaded liposomes and mucin BBA Gen. Subj. (IF 3.0) Pub Date : 2023-11-05 Prabakaran A., Amit Alexander
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CHMP4B and VSP4A reverse GSDMD-mediated pyroptosis by cell membrane remodeling in endometrial carcinoma BBA Gen. Subj. (IF 3.0) Pub Date : 2023-11-04 Ye Yang, Hai-Lian Chen, Su Fang Wu, Wei Bao
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Towards development of biobetter: L-asparaginase a case study BBA Gen. Subj. (IF 3.0) Pub Date : 2023-10-31 Rajan K. Tripathy, J. Anakha, Abhay H. Pande
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Tyrosinase inhibitory peptides: Structure-activity relationship study on peptide chemical properties, terminal preferences and intracellular regulation of melanogenesis signaling pathways BBA Gen. Subj. (IF 3.0) Pub Date : 2023-11-03 Pei-Gee Yap, Chee-Yuen Gan
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Targeted expression of a designed fusion protein containing BMP2 into the lumen of exosomes BBA Gen. Subj. (IF 3.0) Pub Date : 2023-11-03 Maryam Noei-Khesht Masjedi, Esmaeil Sadroddiny, Jafar Ai, Saeed Balalaie, Yazdan Asgari
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Genome-wide identification and expression analysis reveal the role of histone methyltransferase and demethylase genes in heat stress response in potato (Solanum tuberosum L.) BBA Gen. Subj. (IF 3.0) Pub Date : 2023-11-02 Surbhi Mali, Gaurav Zinta
Background Potato (Solanum tuberosum L.), the third most important non-cereal crop, is sensitive to high temperature. Histone modifications have been known to regulate various abiotic stress responses. However, the role of histone methyltransferases and demethylases remain unexplored in potato under heat stress. Methods Potato genome database was used for genome-wide analysis of StPRMT and StHDMA gene