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Myristica fragrans promotes ABCA1 expression and cholesterol efflux in THP-1-derived macrophages Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2021-01-12 Liu S, Gao J, He L, et al.
AbstractMyristica fragrans is a traditional herbal medicine and has been shown to alleviate the development of atherosclerosis. However, the anti-atherogenic mechanisms of M. fragrans are still to be addressed. In this study, we explored the effect of M. fragrans on lipid metabolism and inflammation and its mechanisms in THP-1-derived macrophages. The quantitative polymerase chain reaction and western
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ARRB2 promotes colorectal cancer growth through triggering WTAP Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-12-23 Liang H, Lin Z, Ye Y, et al.
AbstractColorectal cancer (CRC) is one of the most lethal cancers worldwide. The expression of β-arrestin2 (β-Arr2, ARRB2) in CRC has been well investigated; however, its exact mechanism causing the cancer progression remains unclear. In this study, we discovered that the expression level of ARRB2 was significantly upregulated in CRC as compared to the normal tissues by employing the Cancer Genome
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The intrinsic axon regenerative properties of mature neurons after injury Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-12-23 Liu C, Liu J, Liu C, et al.
AbstractThousands of nerve injuries occur in the world each year. Axon regeneration is a very critical process for the restoration of the injured nervous system’s function. However, the precise molecular mechanism or signaling cascades that control axon regeneration are not clearly understood, especially in mammals. Therefore, there is almost no ideal treatment method to repair the nervous system’s
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Immunohistochemical analysis of HNF4A and β-catenin expression to predict low-grade dysplasia in the colitis-neoplastic sequence Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-12-14 He Y, Chen L, Chen K, et al.
AbstractAnimal studies indicated that P1 promoter–driven hepatocyte nuclear factor 4 alpha (HFN4A) prevents carcinogenesis in colitis. But the function of total HNF4A protein has not been fully investigated, and it was assumed to be involved in the colitis-neoplastic sequence. The aim of this study was to determine the clinical value of total P1-/P2-driven HNF4A combined with β-catenin in the colitis-neoplastic
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miR-199b-5p enhances the proliferation of medullary thymic epithelial cells via regulating Wnt signaling by targeting Fzd6 Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-12-12 Wang X, Li Y, Gong B, et al.
AbstractThymic epithelial cells (TECs) are essential regulators of T-cell development and selection. miRNAs play critical roles in regulating TEC proliferation during the process of thymic aging. Our previous studies revealed that miR-199b-5p was upregulated in TECs from 1- to 3-month-old mice. But its function and potential mechanism are not clear. We hypothesized that miR-199b-5p may play an important
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A novel secretagogin/ATF4 pathway is involved in oxidized LDL-induced endoplasmic reticulum stress and islet β-cell apoptosis Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-12-08 Wu L, Lv Y, Lv Y, et al.
AbstractExcessive accumulation of cholesterol in β cells initiates endoplasmic reticulum (ER) stress and associated apoptosis. We have reported that excessive uptake of cholesterol by MIN6 cells decreases the expression of secretagogin (SCGN) and then attenuates insulin secretion. Here, we aimed to determine whether cholesterol-induced SCGN decrease is involved in the modulation of ER stress and apoptosis
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2-Bromopalmitate attenuates inflammatory pain by maintaining mitochondrial fission/fusion balance and function Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-11-30 Xie M, Cheng M, Wang B, et al.
AbstractInflammatory pain activates astrocytes and increases inflammatory cytokine release in the spinal cord. Mitochondrial fusion and fission rely on the functions of dynamin-related protein 1 (Drp1) and optic atrophy 1 (OPA1), which are essential for the synaptic transmission and plasticity. In the present study, we aimed to explore the effects of 2-bromopalmitate (2-BP), an inhibitor of protein
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Piezo1 regulates migration and invasion of breast cancer cells via modulating cell mechanobiological properties Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-11-28 Yu Y, Wu X, Liu S, et al.
AbstractCell migration and invasion are two essential processes during cancer metastasis. Increasing evidence has shown that the Piezo1 channel is involved in mediating cell migration and invasion in some types of cancers. However, the role of Piezo1 in the breast cancer and its underlying mechanisms have not been clarified yet. Here, we show that Piezo1 is high-expressed in breast cancer cell (BCC)
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1,25-(OH)2D3 protects pancreatic beta cells against H2O2-induced apoptosis through inhibiting the PERK-ATF4-CHOP pathway Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-11-26 Hu X, Hu C, Liu J, et al.
AbstractEndoplasmic reticulum (ER) stress plays a critical role in pancreatic β cell destruction which leads to the pathogenesis of type 1 diabetes mellitus (T1DM). Vitamin D (VD) has been reported to reduce the risk of T1DM; however, it remains unknown whether VD affects ER stress in pancreatic β cells. In this study, we investigated the role of the active form of VD, 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]
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AMPKα1 regulates Idh2 transcription through H2B O-GlcNAcylation during brown adipogenesis Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-11-21 Cao Y, Liu X, Zhao J, et al.
AbstractAMP-activated protein kinase (AMPK) is indispensable for the development and maintenance of brown adipose tissue (BAT), and its activity is inhibited due to obesity. The isocitrate dehydrogenase 2 (IDH2) is a mitochondrial enzyme responsible for the production of α-ketoglutarate, a key intermediate metabolite integrating multiple metabolic processes. We previously found that AMPKα1 ablation
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Proscillaridin A inhibits hepatocellular carcinoma progression through inducing mitochondrial damage and autophagy Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-11-17 Luo M, Liu Y, Liu N, et al.
AbstractHepatocellular carcinoma (HCC) is one of the leading causes of cancer-related deaths globally. At present, drug options for systemic treatment of HCC are very limited. There is an urgent need to develop additional effective drugs for HCC treatment. In the present study, we found that proscillaridin A (ProA), a cardiac glycoside, exerted a strong anticancer effect on multiple HCC cell lines
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Combined chemotherapy of platinum and fluorouracil promotes T cell–mediated antitumor immunity Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-11-17 Luo Y, Pei S, Xu J, et al.
AbstractThe two-drug combined chemotherapy of platinum and fluorouracil has been reported to efficiently kill tumor cells as the first-line treatment for advanced gastric cancer. However, the effect of these drugs on T cells remains unclear. Here, we showed that T cells including CD4+ T cells and CD8+ T cells of the patients with advanced gastric cancer after platinum and fluorouracil chemotherapy
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Cardiovascular disease in patients with COVID-19: evidence from cardiovascular pathology to treatment Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2021-01-11 Jinwen Luo; Xiao Zhu; Jie Jian; X u Chen; Kai Yin
The coronavirus disease-2019 (COVID-19) caused by the novel coronavirus severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has rapidly developed into a global pneumonia pandemic. At present, COVID-19 has caused more than 70,000,000 confirmed cases with over 1,500,000 deaths worldwide, as reported by WHO. Cardiovascular disease is the major comorbidity of COVID-19 patients and is closely related
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Tamoxifen attenuates reactive astrocyte-induced brain metastasis and drug resistance through the IL-6/STAT3 signaling pathway Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-12-23 Xu Y, Zhu Y, Yue Y, et al.
AbstractBrain metastasis affects approximately 20%–30% of patients with triple-negative breast cancers (TNBCs). Even small metastatic lesions in the brain can trigger severe neurological impairments and result in extremely short survival time. Recently, active astrocytes were reported to be associated with brain metastases. However, how activated astrocytes regulate the behaviors of disseminated breast
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Preosteoblast-enriched lnc-Evf2 facilitates osteogenic differentiation by targeting Notch Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-12-30 Zhen Zhang; Haixia Qi; Han Xia; Q i Liu; Y i Ren; Kun Zhang; Yuan Xue; Wei Hong
Ossification of ligaments (OL) and osteoporosis (OP) are multifactorial disorders without definitive clinical biomarkers. Long non-coding RNAs (lncRNAs) are known to involve in regulating pathogenesis. Here, we have identified a preosteoblast-enriched lnc-Evf2 that was overexpressed in ossified ligamentum flavum (OLF) and down-expressed in OP. lnc-Evf2 is gradually upregulated during osteogenic induction
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Development of a dual-fluorescence reporter system for high-throughput screening of L-aspartate-α-decarboxylase Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-12-14 Fei M, Mao X, Chen Y, et al.
Abstractβ-Alanine (3-aminopropionic acid) holds great potential in industrial application. It can be obtained through a chemical synthesis route, which is hazardous to the environment. It is well known that l-aspartate-α-decarboxylase (ADC) can convert l-aspartate to β-alanine in bacteria. However, due to the low activity of ADC, industrial production of β-alanine through the green biological route
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LncRNA HOTAIR promotes endometrial fibrosis by activating TGF-β1/Smad pathway Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-12-12 Wu J, Jin L, Zhang Y, et al.
AbstractHomeobox transcript antisense RNA (HOTAIR) is a long non-coding RNA associated with a number of fibrosis-related diseases. The aim of this study was to investigate the specific role of HOTAIR in the development of endometrial fibrosis and to identify the molecular mechanisms underlying this process. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to determine
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AT1-receptor autoantibody exposure in utero contributes to cardiac dysfunction and increased glycolysis in fetal mice Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-11-21 Bai L, Wang M, Zhang S, et al.
AbstractExposure to adverse factors in utero may lead to adaptive changes in cardiac structure and metabolism, which increases the risk of chronic cardiovascular disease later in life. Studies showed that the angiotensin II type 1 receptor autoantibodies (AT1-AAs) are able to cross the placenta into the circulation of pregnant rodents’ embryo, which adversely affects embryogenesis. However, the effects
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N-Glycosylation at Asn695 might suppress inducible nitric oxide synthase activity by disturbing electron transfer Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-11-21 Yan J, Shang F, He A, et al.
AbstractInducible nitric oxide synthase (iNOS) plays critical roles in the inflammatory response and host defense. Previous research on iNOS regulation mainly focused on its gene expression level, and much less is known about the regulation of iNOS function by N-glycosylation. In this study, we report for the first time that iNOS is N-glycosylated in vitro and in vivo. Mass spectrometry studies identified
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Improved EGFR mutation detection sensitivity after enrichment by Cas9/sgRNA digestion and PCR amplification Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-11-20 Wang L, Jiang J, Li X, et al.
AbstractThe detection of circulating tumor DNA is important in cancer research and clinical practice. In the present study, we aimed to improve the sensitivity of downstream mutation detection of next-generation sequencing using the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system to selectively target wild-type fragments but with low or no
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Exogenous hydrogen sulfide inhibits apoptosis by regulating endoplasmic reticulum stress–autophagy axis and improves myocardial reconstruction after acute myocardial infarction Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-11-19 Li Y, Liu M, Yi J, et al.
AbstractDuring acute myocardial infarction, endoplasmic reticulum (ER) stress-induced autophagy and apoptosis have been shown as important pathogeneses of myocardial reconstruction. Importantly, hydrogen sulfide (H2S), as a third endogenous gas signaling molecule, exerts strong cytoprotective effect on anti-ER stress, autophagy regulation and antiapoptosis. Here, we showed that H2S treatment inhibits
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Rapid and accurate detection of African swine fever virus by DNA endonuclease-targeted CRISPR trans reporter assay Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-11-17 Li Z, Wei J, Di D, et al.
AbstractThe first case of African swine fever (ASF) outbreak in China was reported in a suburban pig farm in Shenyang in 2018. Since then, the rapid spread and extension of ASF has become the most serious threat for the swine industry. Therefore, rapid and accurate detection of African swine fever virus (ASFV) is essential to provide effective strategies to control the disease. In this study, we developed
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miR-4530 inhibits the malignant biological behaviors of human glioma cells by directly targeting RTEL1 Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-11-17 Wang T, Zhang Y, Cui B, et al.
AbstractHuman glioma is the most common primary brain tumor and is associated with high morbidity and mortality. Aberrant expressions of microRNAs (miRNAs) are involved in glioma progression. In the present study, we aimed to elucidate the roles of miR-4530 in the pathogenesis of gliomas. miR-4530 expression was examined in human glioma clinical tissues and cell lines including U251 and T98G. The target
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miR-3677-3p promotes hepatocellular carcinoma progression via inhibiting GSK3β Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-11-17 Li Y, Zhou Y, Ma L, et al.
AbstractMicroRNAs play important roles in regulating hepatocellular carcinoma (HCC) formation, progression and metastasis. However, their functions and the underlying molecular mechanisms are still unclear. Here, we found that miR-3677-3p was highly expressed in primary tumor tissues of HCC patients. And its inhibition by using sponge in HCC cells could suppress cell proliferation significantly, but
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N6-methyladenosine methyltransferase plays a role in hypoxic preconditioning partially through the interaction with lncRNA H19 Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-11-17 Su Y, Xu R, Zhang R, et al.
AbstractN6-methyladenosine (m6A), a methylation in the N6 position of adenosine especially in the mRNA, exerts diverse physiological and pathological functions. However, the precise role of m6A methylation in hypoxic preconditioning (HPC) is still unknown. Here, we observed that HPC treatment protected H9c2 cells against H2O2-induced injury, upregulated the m6A level in the total RNA and the expression
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Exposure to dibutyl phthalate impairs lipid metabolism and causes inflammation via disturbing microbiota-related gut–liver axis Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-11-10 Xiong Z, Zeng Y, Zhou J, et al.
AbstractDibutyl phthalate (DBP), a kind of typical environmental pollutant, is widely used as plasticizers, and its neurotoxicity and developmental toxicity have been found in recent years. However, whether oral DBP exposure will affect the homeostasis of gut microbiota and its adverse response in liver of mammalians remain unclear. In the present study, 10-week experimental cycles of vehicle or DBP
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FOXO1 and FOXO3a sensitize non-small-cell lung cancer cells to cisplatin-induced apoptosis independent of Bim Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-11-10 Sun T, Zhang J, Deng B, et al.
AbstractLow sensitivity to chemotherapy has been a major challenge in the treatment of non-small-cell lung cancer (NSCLC). It is of great clinical significance to discover its mechanisms to improve cell sensitivity to chemotherapeutic drugs. The forkhead box subfamily O (FOXO) transcriptional factors are downstream factors of the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) pathway and
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miR-20b suppresses mitochondrial dysfunction–mediated apoptosis to alleviate hyperoxia-induced acute lung injury by directly targeting MFN1 and MFN2 Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-12-21 Genhua Mu; Yijun Deng; Zhongqian Lu; Xing Li; Yanbin Chen
Supplemental oxygen is commonly used to treat severe respiratory failure, while prolonged exposure to hyperoxia can induce acute lung injury characterized by the accumulation of reactive oxygen species (ROS) and pulmonary inflammation. Dysregulation of microRNAs contributes to multiple diseases, including hyperoxia-induced acute lung injury (HALI). In this study, we explored the roles of miR-20b in
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Molecular evolution and expression pattern of Toll-like receptor 3 from the lamprey Lampetra japonica Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-12-21 Jian Quan; Ming Geng; Xueting Hu; Shaoshu Feng; Yu Liu; Fan Hui; Meiao Li; Jinzhao Liu; Peng Su; Xin Liu; Qingwei Li; Ting Zhu
AbstractNFκB1/p105 is the critical member of the NFκB family which can suppress inflammation, ageing, and cancer when p50/p50 homodimer is formed. Currently, the research about the role of NFκB1/p105 during cow mastitis is limited. Here, we analyzed the correlation of six single-nucleotide variants of the NFκB1 gene with somatic cell count, milk yield, milk fat content, and milk protein content in
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Preparation, characterization, and evaluation of antioxidant activity and bioavailability of a self-nanoemulsifying drug delivery system (SNEDDS) for buckwheat flavonoids Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-11-20 Zhao Z, Cui X, Ma X, et al.
AbstractThe self-nanoemulsifying drug delivery system has shown many advantages in drug delivery. In this study, a self-nanoemulsifying drug delivery system of buckwheat flavonoids was prepared for enhancing its antioxidant activity and oral bioavailability. A nanoemulsion of buckwheat flavonoids was developed and characterized, and its antioxidant, in vitro release, and in vivo bioavailability were
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DJ-1 is involved in the multidrug resistance of SGC7901 gastric cancer cells through PTEN/PI3K/Akt/Nrf2 pathway Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-10-30 Qiu L, Ma Z, Li X, et al.
AbstractGastric cancer is a common malignancy worldwide. The occurrence of multidrug resistance (MDR) is the major obstacle for effective gastric cancer chemotherapy. In this study, the in-depth molecular mechanism of the DJ-1-induced MDR in SGC7901 gastric cancer cells was investigated. The results showed that DJ-1 expression level was higher in MDR variant SGC7901/VCR cells than that in its parental
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DNMT3A inhibits E2F1-induced arterial marker expression and impairs angiogenesis in human umbilical artery endothelial cells Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-10-30 Su K, Lin N, Xie S, et al.
AbstractArterial marker genes EphrinB2 and HEY2 are essential for cardiovascular development and postnatal neovascularization. Our previous study confirmed that E2F1 could activate the transcription of EphrinB2 and HEY2 in human mesenchymal stem cells; however, the detailed mechanism has not been resolved yet. In this study, we focused on the interaction between E2F1 and DNMT3A, a de novo DNA methyltransferase
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Molecular diversification of antimicrobial peptides from the wolf spider Lycosa sinensis venom based on peptidomic, transcriptomic, and bioinformatic analyses Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-10-22 Tang X, Yang J, Duan Z, et al.
AbstractThe venom of Lycosoidea spiders is a complex multicomponent mixture of neurotoxic peptides (main components) and antimicrobial peptides (AMPs) as minor components. In this study, we described the high-throughput identification and analysis of AMPs from Lycosa sinensis venom (named LS-AMPs) using a combination strategy that includes the following three different analysis approaches: (i) peptidomic
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Artificial intelligence modeling of vascular calcification Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-10-30 Mingjun Gu; Lin zhou; Xing Wang; Chao Shi; Xiangqi Li
Vascular calcification is a pathological process during which deposits of calcium mineral in vessel walls are progressively formed, leading to tissue stiffening, plaque rupture, and heart failure [1]. The mortality rate is 3% among non-calcification individuals, while it reaches 28% in patients with high levels of vascular calcification [2]. Despite rapid advances in the understanding of this health
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Transcriptome-wide alternative polyadenylation profiling reveals the herbal formula Yangyinjiedu-induced preferential poly(A) usage in lung adenocarcinoma cells Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-10-21 Qiang Zhao; Xinglong Shi; Yucan Chen; Ling Bi; Jing Tian; Ming Zhang; Ling Xu; Jielin Sun; Xiaodong Zhao
† These authors contributed equally to this work.
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Dihydroartemisinin induces ER stress-dependent apoptosis of Echinococcus protoscoleces in vitro Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-10-21 Rongji Ma; Wenjuan Qin; Yuanmao Xie; Ziwei Han; Shuojie Li; Yufeng Jiang; Hailong Lv
In this study, we investigated the effect of dihydroartemisinin on Echinococcus protoscoleces and explored the role of endoplasmic reticulum stress in this process. Echinococcus protoscoleces were collected and cultured in RPMI 1640 medium. Changes in the expressions of glucose-regulated protein 78 (GRP-78), caspase-12, and C/EBP homologous protein (CHOP) were assessed through confocal immunofluorescence
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ROS signaling cascades: dual regulations for osteoclast and osteoblast Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-10-21 Huaqiang Tao; Gaoran Ge; Xiaolong Liang; Weicheng Zhang; Houyi Sun; Meng Li; Dechun Geng
Accumulating evidence indicates that intracellular reactive oxygen species (ROS) production is highly involved in bone homeostasis by intervening osteoclast or osteoblast differentiation. Interestingly, ROS that are known as oxidizing agents exert dose-dependent biphasic properties in bone remodeling, including preventing osteoblast activity but accelerating osteoclast resorption. ROS mainly composed
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A novel mannose-binding lectin from Liparis nervosa with anti-fungal and anti-tumor activities. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-08-27 Na Jiang,Yuqing Wang,Jing Zhou,Ruxiao Zheng,Xiao Yuan,Miaomiao Wu,Jinku Bao,Chuanfang Wu
AbstractPlant lectins are carbohydrate-binding proteins with nonimmune origin, which can reversibly bind with carbohydrates, agglutinate cells, and precipitate polysaccharides and glycoconjugates. Plant lectins have attracted much attention for their anti-virus, anti-proliferation, and pro-apoptosis properties. Thus the exploration of new lectins has received special attention. Here we purified a mannose-binding
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Quercetin promotes human epidermal stem cell proliferation through the estrogen receptor/β-catenin/c-Myc/cyclin A2 signaling pathway. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-08-25 Zhaodong Wang,Guangliang Zhang,Yingying Le,Jihui Ju,Ping Zhang,Dapeng Wan,Qiang Zhao,Guangzhe Jin,Hao Su,Jinwei Liu,Jiaxuan Feng,Yi Fu,Ruixing Hou
AbstractSkin epidermal stem cells (EpSCs) play an important role in wound healing. Quercetin is a phytoestrogen reported to accelerate skin wound healing, but its effect on EpSCs is unknown. In this study, we investigated the effect of quercetin on human EpSC proliferation and explored the underlying mechanisms. We found that quercetin at 0.1~1 μM significantly promoted EpSC proliferation and increased
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IRP2-Hif1α/Hif2α signaling: a novel mechanism of metabolic switch from aerobic glycolysis to oxidative phosphorylation. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-08-19 Li Zhu,Qionglin Zhou,Lu He,Linxi Chen
Iron regulatory protein 2 (IRP2), first separated from mouse in 1993, is a cytoplasmic iron-regulated RNA-binding protein. IRP2 is a subtype of iron regulatory proteins (IRPs). IRP2 binds to iron-responsive element (IRE) RNA sequence to maintain iron homeostasis [1]. IRP2 can easily be hydrolyzed by protease because of its unique sequence of 73 amino acids. In general, IRP2 is widely expressed in many
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Pannexin-2, a novel mitochondrial-associated membrane protein, may become the new strategy to treat and prevent neurological disorders. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-08-08 Yiyuan Yang,Li Wang,Linxi Chen,Lanfang Li
Connexins and pannexins are two kinds of transmembrane proteins, which play important physiological roles. Connexins form hemichannels and channels, mainly contributing to the formation of gap junctions. Pannexins are newly discovered gap junction proteins and their structures consist of intracellular N-termini, intracellular C termini, an intracellular loop, two extracellular loops, and four transmembrane
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STAT3 and AKT signaling pathways mediate oncogenic role of NRSF in hepatocellular carcinoma. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-06-18 Ming Ma,Yunhe Zhou,Ruilin Sun,Jiahao Shi,Yutong Tan,Hua Yang,Mengjie Zhang,Ruling Shen,Leon Xu,Zhugang Wang,Jian Fei
AbstractNeuron-restrictive silencer factor (NRSF) is a zinc finger protein that acts as a negative transcriptional regulator by recruiting histone deacetylases and other co-factors. It plays a crucial role in nervous system development and is recently reported to be involved in tumorigenesis in a tumor type-dependent manner; however, the role of NRSF in hepatocellular carcinoma (HCC) tumorigenesis
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iASPP protects the heart from ischemia injury by inhibiting p53 expression and cardiomyocyte apoptosis Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-10-30 Yagudin T, Zhao Y, Gao H, et al.
AbstractCurrently, there remains a great need to elucidate the molecular mechanism of acute myocardial infarction in order to facilitate the development of novel therapy. Inhibitor of apoptosis-stimulating protein of p53 (iASPP) is a member of the ASPP family proteins and an evolutionarily preserved inhibitor of p53 that is involved in many cellular processes, including apoptosis of cancer cells. The
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Comparison of the differentiation of dental pulp stem cells and periodontal ligament stem cells into neuron-like cells and their effects on focal cerebral ischemia. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-08-26 Tingting Wu,Wanting Xu,Hanlin Chen,Shasha Li,Rengang Dou,Hongtao Shen,Xue Liu,Xiaoyu Liu,Yongfeng Hong,Jiacai He
Recent studies have reported an increasing incidence of ischemic stroke, particularly in younger age groups. Dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) are the most common stem cells acquired from the teeth of adults, even elderly people. However, there are no detailed reports on whether DPSCs or PDLSCs are suitable for the treatment of ischemic stroke. In this study
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NEAT1/miR-140-3p/MAPK1 mediates the viability and survival of coronary endothelial cells and affects coronary atherosclerotic heart disease. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-08-26 Hui Zhang,Ningning Ji,Xinyan Gong,Shimao Ni,Yu Wang
Studies have shown that long non-coding RNAs (lncRNA) play critical roles in coronary atherosclerotic heart disease (CAD). However, the function of lncRNA nuclear enriched abundant transcript 1 (NEAT1) in CAD is unclear. In this study, we aimed to investigate the functions of lncRNA NEAT1 in CAD. RT-PCR and western blot analysis were carried out to examine the expressions of related RNAs. Colony formation
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Mitochondrial metabolism in regulating macrophage polarization: an emerging regulator of metabolic inflammatory diseases. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-08-12 Jina Qing,Zizhen Zhang,Petr Novák,Guojun Zhao,Kai Yin
As a major type of immune cells with heterogeneity and plasticity, macrophages are classically divided into inflammatory (M1) and alternative/anti-inflammatory (M2) types and play a crucial role in the progress of the inflammatory diseases. Recent studies have shown that metabolism is an important determinant of macrophage phenotype. Mitochondria, one of the most important compartments involving cell
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Abnormal expression of homeobox c6 in the atherosclerotic aorta and its effect on proliferation and migration of rat vascular smooth muscle cells. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-08-12 Xiangshu Long,Ganhua You,Qiang Wu,Yu Zhou,Fuxun Yu,Yan Xiao,Shiyan Deng,Fang Song,Jing Huang,Maobo Tian
Homeobox c6 (Hoxc6) affects the proliferation, migration, and infiltration of malignant tumor cells; however, the effect of Hoxc6 on atherosclerosis (AS) as well as the proliferation and migration of vascular smooth muscle cells (VSMCs), which play a role in promoting AS, has not yet been well clarified. In the present study, we tested the hypothesis that Hoxc6 affects the proliferation and migration
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Guanine nucleotide-binding protein subunit beta-4 promotes gastric cancer progression via activating Erk1/2. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-08-04 Jianpeng Gao,Hongda Pan,Zhenglun Zhu,Teng Yu,Binhao Huang,Ye Zhou
Gastric cancer (GC) is one of the most common and lethal malignancies worldwide, and its poor prognosis is mainly due to the rapid tumor progression including tumor invasion, distant metastasis, etc. Understanding the molecular mechanisms regulating GC progression lays the basis for the development of targeted therapeutic agents. Increasing evidence suggests that guanine nucleotide-binding protein
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Identification of host protein CBL interacting with Eimeria acervulina microneme protein MIC3. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-07-31 Pu Wang,Hanxiao Zhao,Qi Wang,Chenyang Gao,Haihong Wu,Changyong Cheng,Houhui Song
Eimeria acervulina severely damages the intestinal mucosa in infected poultry, resulting in major economic losses [1]. The study of the interaction between Eimeria and host cells is the theoretical basis for the prevention and treatment of coccidiosis [2]. However, the specific mechanism of E. acervulina invasion is not very clear, so the interaction between the invading protein and the host awaits
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Identification of novel miRNAs in mouse embryonic stem cells and reprogrammed pluripotent cells. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-07-30 Botao Zhao,Chunsun Fan,Youxin Jin
MicroRNAs (miRNAs) are a class of endogenous small RNAs that regulate target gene expression at the post-transcriptional level. miRNAs play an important role in almost all physiological activities including the processes of embryonic stem cell (ESC) pluripotency maintenance and differentiation, as well as induced pluripotent stem cell (iPSC) reprogramming. In this study, we identified eight novel miRNAs
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Comprehensive analysis of circular RNA expression profiles in cisplatin-resistant non-small cell lung cancer cell lines. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-07-27 Lin Song,Zhilei Cui,Xuejun Guo
Platinum-based drugs such as cisplatin are widely used in combination chemotherapy for non-small cell lung cancer (NSCLC) owing to their high clinical response rate; however, acquired resistance to cisplatin is eventually inevitable. Circular RNAs (circRNAs) are involved in the development of diverse types of cancers, but their connection to cisplatin-resistance in NSCLC has not been studied. In the
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Baicalein suppresses vasculogenic mimicry through inhibiting RhoA/ROCK expression in lung cancer A549 cell line. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-07-16 Zhe Zhang,Li Nong,Menglei Chen,Xiaoli Gu,Weiwei Zhao,Minghui Liu,Wenwu Cheng
Vasculogenic mimicry (VM) refers to a new tubular network of the blood supply system with abundant extracellular matrix. VM is similar to capillaries but does not involve endothelial cells. As a traditional herbal medicine commonly used in China, baicalein possesses anti-inflammatory and lipoxygenase activities. However, the effects of baicalein on the process of VM formation in non-small cell lung
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Corrigendum to: Gene expression profiling of cells of origin of squamous cell carcinomas in head-and-neck, esophagus, and lung. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-07-09 Ning Qu,Dongzhe Huang,Qinghua Xu,Jian Wang,Chengshu Chen,Qinghai Ji,Weili Wu,Yongxue Zhu,Midie Xu
In the original version of this manuscript, the funding ID for the Shanghai International Science and Technology Cooperation Fund was incorrect. The ID has now been corrected to 18410742000. The author apologizes for the error.
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Upregulation of miR-133a by adiponectin inhibits pyroptosis pathway and rescues acute aortic dissection. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-07-07 Haizhen Duan,Xiaojun Zhang,Renjie Song,Tongying Liu,Yuanyuan Zhang,Anyong Yu
Acute aortic dissection (AAD) is a cardiovascular emergency caused by the formation of hematoma in the middle layer of the aortic wall. Adiponectin (APN) is an adipose tissue-specific protein that has anti-inflammation and anti-atherosclerosis functions. Pyroptosis, as an inflammatory cell death, depends on the activation of caspase1, while nucleotide-binding oligomerization domain-like receptor protein
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HEATR1 promotes proliferation in gastric cancer in vitro and in vivo. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-07-07 Jun Zhao,Yiping Zhu,Qingsheng Fu,Yimei Zhu,Guohai Zhao
HEAT repeat-containing protein 1 (HEATR1) is related to the progression of several cancers. However, the role of HEATR1 in gastric cancer (GC) remains unknown. In the present study, we aimed to detect the expression of HEATR1 in GC and identify its role. The expressions of HEATR1 in GC tissues were analyzed using The Cancer Genome Atlas database and by western blot analysis and immunohistochemistry
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ZnT8 in T2D: a novel therapeutic target for maintaining insulin secretion capacity. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-07-07 Yao Zhang,Wu Jian,Lu He,Jianhua Wu
Type 2 diabetes (T2D) is the most prevalent form of the diabetes mellitus with a defect of insulin secretion and hyperglycemia, imposing immense health and economic burdens for nearly 400 million people worldwide [1]. It is often caused by a combination of lifestyle and genetic factors that raises blood glucose level with ensuing insulin resistance in the liver, skeletal muscles, and adipose tissue
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Cloning, characterization, and functional analysis of chitinase-like protein 1 in the shell of Pinctada fucata. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-07-07 Yunpin Zhou,Yi Yan,Dong Yang,Guilan Zheng,Liping Xie,Rongqing Zhang
Biomineralization, especially shell formation, is a sophisticated process regulated by various matrix proteins. Pinctada fucata chitinase-like protein 1 (Pf-Clp1), which belongs to the GH18 family, was discovered by our group using in-depth proteomic analysis. However, its function is still unclear. In this study, we first obtained the full-length cDNA sequence of Pf-Clp1 by RACE. Real-time polymerase
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Characteristics of lipid metabolism-related gene expression-based molecular subtype in papillary thyroid cancer. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-09-03 Midie Xu,Tuanqi Sun,Shishuai Wen,Tingting Zhang,Xin Wang,Yiming Cao,Yu Wang,Xing Sun,Qinghai Ji,Rongliang Shi,Ning Qu
Metabolic reprogramming has been recognized as one of the 10 new hallmarks of cancer cells [1]. Increased glycolysis (Warburg effect) under normoxic conditions and glutamine metabolism has been well recognized as the main characteristics of human malignancies [2]. Cumulative evidence supports the concept that dysregulation of lipid metabolism is also one of the key metabolic markers of cancer cells
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JNK inhibition alleviates oxidative DNA damage, germ cell apoptosis, and mitochondrial dysfunction in testicular ischemia reperfusion injury. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-07-14 Fatemah Fadel,Nora Al-Kandari,Farah Khashab,Farah Al-Saleh,May Al-Maghrebi
The aim of this study is to determine whether the c-Jun N-terminal kinase (JNK) signaling is a regulator of oxidative DNA damage, germ cell apoptosis (GCA), and mitochondrial dysfunction during testicular ischemia reperfusion injury (tIRI) using the JNK inhibitor SP600125. Male Sprague Dawley rats (n = 36) were equally divided into three groups: sham, tIRI only, and tIRI + SP600125 (15 mg/kg). Testicular
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miR-28-5p targets MTSS1 to regulate cell proliferation and apoptosis in esophageal cancer. Acta Biochim. Biophys. Sin. (IF 2.836) Pub Date : 2020-07-09 Liang Zhang,Xin Wang,Xin Liu,Mingyue Lv,Erdong Shen,Guolian Zhu,Zhe Sun
Esophageal cancer (EC) is one of the most common aggressive malignant diseases worldwide. miR-28-5p plays important regulatory roles in many cancers including human EC. However, the molecular mechanism and potential role of miR-28-5p in EC remain uncertain. In this study, qRT-PCR and western blot analysis revealed that miR-28-5p expression was up-regulated and metastasis suppressor-1 (MTSS1) was down-regulated
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