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The decrease of fucosylation in intestinal epithelium is related to the development of necrotizing enterocolitis Mol. Immunol. (IF 3.641) Pub Date : 2021-01-16 Xiang She; Hua Du; Cong Yi; Yu He; Qing Ai; Jialin Yu
Necrotizing enterocolitis (NEC) is a devastating neonatal gastrointestinal emergency. Fucosylated glycans on intestinal epithelial cells (IECs) play a central role in the maintenance of intestinal homeostasis. Nevertheless, its association with necrotizing enterocolitis is not clear. We examined paraffin-embedded intestinal specimens from participants and found that the NEC patients showed lower intestinal
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Dehydroepiandrosterone attenuates LPS-induced inflammatory responses via activation of Nrf2 in RAW264.7 macrophages Mol. Immunol. (IF 3.641) Pub Date : 2021-01-16 Ji Cao; Qian Li; Xuehuai Shen; Yao Yao; Longlong Li; Haitian Ma
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Potential therapeutic effects of interleukin-35 on the differentiation of naïve T cells into Helios+Foxp3+ Tregs in clinical and experimental acute respiratory distress syndrome Mol. Immunol. (IF 3.641) Pub Date : 2021-01-16 Chuanjiang Wang; Ke Xie; Kefeng Li; Shihui Lin; Fang Xu
Regulatory T lymphocytes are important targets for the treatment of acute respiratory distress syndrome (ARDS). IL-35 is a newly identified IL-12 cytokine family member that plays an important protective role in a variety of immune system diseases by regulating Treg cell differentiation; however, the role of IL-35 in the pathogenesis of ARDS is still unclear. Here, we found that IL-35 was significantly
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TREM2 alters the phagocytic, apoptotic and inflammatory response to Aβ42 in HMC3 cells Mol. Immunol. (IF 3.641) Pub Date : 2021-01-15 Rumana Akhter; Yvonne Shao; Shane Formica; Maria Khrestian; Lynn M. Bekris
Alzheimer's disease (AD) is characterized by the accumulation in the brain of extracellular amyloid β (Aβ) plaques as well as intraneuronal inclusions (neurofibrillary tangles) consisting of total tau and phosphorylated tau. Also present are dystrophic neurites, loss of synapses, neuronal death, and gliosis. AD genetic studies have highlighted the importance of inflammation in this disease by identifying
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Identification of nanobodies against hepatocellular carcinoma marker glypican-3 Mol. Immunol. (IF 3.641) Pub Date : 2021-01-13 Wenyi Wang; Chang Xu; Huanan Wang; Changan Jiang
Glypican-3 (GPC3) is a highly specific diagnostic marker for hepatocellular carcinoma (HCC) diagnosis and a potential target in HCC therapy. Nanobodies (Nbs) are promising targeting molecules due to their high specificity and strong affinities to antigens, high stability, deep tissue penetration, and low immunogenicity. In this study, we isolated Nbs against GPC3 marker protein from a synthetic Nb
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Effects of CpG oligodeoxynucleotides on the differentiation of Treg/Th17 cells Mol. Immunol. (IF 3.641) Pub Date : 2021-01-13 Hailing Liu; Yuqiang Ji; Xiaorong Ma; Aili He; Wanhong Zhao; Pengyu Zhang; Liufang Gu; Bo Lei; Yilin Zhang; Yueli Wang; Wanggang Zhang; Jin Wang
Aim The balance between Th17 cells and T regulatory (Treg) cells has emerged as a prominent factor in regulating cancer development. However, the effect of CpG oligodeoxynucleotides (ODNs) on the differentiation of Treg/Th17 cells has not been well studied. We sought here to explore the function of CpG ODNs in the differentiation of Tregs and Th17 cells in vitro and in vivo. Methods Mouse spleen cells
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CD226 deficiency attenuates the homeostasis and suppressive capacity of Tr1 cells Mol. Immunol. (IF 3.641) Pub Date : 2021-01-13 Ning Wang; Ping Chen; Yun Song; Yuting Shen; Juan Li; Xinyu Li; Liang Fang; Lihua Chen
T regulatory type 1 (Tr1) cells act as a key regulator in maintaining peripheral immune tolerance. Several costimulatory molecules for T cells have been identified in Tr1 cells, but their intrinsic functions are still unclear. Here we showed CD226 was highly expressed in Tr1 cells. CD226-deficient Tr1 cells were defective in proliferation and sensitive to apoptosis. In addition, CD226-deficient Tr1
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Comparative research on nucleocapsid and spike glycoprotein as the rapid immunodetection targets of COVID-19 and establishment of immunoassay strips Mol. Immunol. (IF 3.641) Pub Date : 2021-01-09 Dan Liu; Feng Wu; Yu Cen; Lei Ye; Xueyin Shi; Yulan Huang; Shisong Fang; Lan Ma
The SARS-CoV-2 virus responsible for coronavirus 2019 (COVID-19) poses a significant challenge to healthcare systems worldwide. According to the World Health Organization (WHO), the outbreak of COVID-19 has been a pandemic that infected more than 25.32 million people and caused more than 848.25 thousand deaths worldwide at the time of 1st September 2020. Despite governmental initiatives aimed to contain
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Expanded circulating peripheral helper T cells in primary biliary cholangitis Mol. Immunol. (IF 3.641) Pub Date : 2021-01-11 Liu Yong; Wang Chunyan; Yu Yan; Li Wanyu; Ji Huifan; Zhao Pingwei; Jiang Yanfang
Background Peripheral helper T (TPH) cells, a recently defined subset of Th cells, promote B cell differentiation and antibody production in inflamed tissues. This study investigated whether circulating TPH cells are associated with primary biliary cholangitis (PBC), a typical organ-specific autoimmune disease. Methods Twenty PBC patients and 20 age- and sex-matched healthy controls (HCs) were recruited
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Exosomes expressing neuronal autoantigens induced immune response in antibody-positive autoimmune encephalitis Mol. Immunol. (IF 3.641) Pub Date : 2021-01-11 Jiachen Gu; Tao Jin; Zongshan Li; Huimin Chen; Hongbo Xia; Xiaomin Xu; Yaxing Gui
The immunological role of exosomes in autoimmune encephalitis (AE) remains uncharacterized and not examined. In this study we ought to determine whether exosomes are generated in AE and to define the presence of cell surface neuronal autoantigens (autoAgs) in the cargo. Exosomes were isolated from cerebrospinal fluid (CSF) from 12 patients with anti-N-methyl-d-aspartate (NMDA) receptor encephalitis
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Proteomic approaches to drive advances in helminth extracellular vesicle research Mol. Immunol. (IF 3.641) Pub Date : 2021-01-10 Karen J. Montaño; Alex Loukas; Javier Sotillo
Helminths can interact with their hosts in many different ways, including through the secretion of soluble molecules (such as lipids, glycans and proteins) and extracellular vesicles (EVs). The field of helminth secreted EVs has significantly advanced in recent years, mainly due to the molecular characterisation of EV proteomes and research highlighting the potential of EVs and their constituent molecules
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miR-143/145 inhibits Th9 cell differentiation by targeting NFATc1 Mol. Immunol. (IF 3.641) Pub Date : 2021-01-11 Xin Qiu; Qiuyue Shi; Youyi Huang; Haixing Jiang; Shanyu Qin
Th9 cells are a defined CD4+ helper T cell subgroup found to promote or suppress oncogenesis in a context-dependent manner. How microRNAs (miRNAs) shape Th9 cell functionality, however, remains to be studied. Herein, we determined that miR-143/145 is downregulated during Th9 differentiation. When these miRNAs were upregulated, this inhibited Th9 differentiation, proliferation, and IL-9 production.
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Functional crosstalk between Long non-coding RNAs and the NLRP3 inflammasome in the regulation of diseases Mol. Immunol. (IF 3.641) Pub Date : 2021-01-11 Deqiang Luo; Fen Liu; Jianguo Zhang; Qiang Shao; Wenqiang Tao; Rui Xiao; Wei Dai; Kejian Qian
Emerging evidence has indicated that long noncoding RNAs (lncRNAs) are involved in various pathophysiological processes of disease, such as cancer occurrence, viral invasion, and inflammatory damage. The main inflammatory body component, nod-like receptor protein 3 (NLRP3), is the trigger point of inflammatory reactions and inflammation-related diseases and coordinates the body's response to inflammation
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Autoantibodies against C5aR1, C3aR1, CXCR3, and CXCR4 are decreased in primary Sjogren’s syndrome Mol. Immunol. (IF 3.641) Pub Date : 2021-01-11 Xiaoyang Yue; Fengyuan Deng; Juan Chen; Junping Yin; Junfeng Zheng; Yan Chen; Qiaoniang Huang; Xing Gao; Zuguo Liu; Jiao Luo; Antje Müller; Harald Heidecke; Gabriela Riemekasten; Frank Petersen; Xinhua Yu
Background Networks formed of numerous autoantibodies (aabs) directed against G-protein coupled receptors (GPCR) have been suggested to play important role in autoimmune disorders. In present study, we aimed to evaluate the association between anti-GPCR antibodies and primary Sjogren’s syndrome (pSS) to determine the potential pathogenic factors. Methods By applying a cell membrane-based ELISA technique
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Methodological considerations for the enrichment of bone marrow endothelial and mesenchymal stromal cells Mol. Immunol. (IF 3.641) Pub Date : 2021-01-10 Evan L. Dray; Carey G. Ousley; Daniel B. McKim
Stromal cells are critical regulators of bone marrow hematopoietic niches, but assessment of their regulatory roles has been impeded by difficult and ineffective dissociation methods. Here, we methodically address bone marrow stromal cell dissociation. Yield of bone marrow CD45−/Ter119−/CD31+/CD202b+ endothelial cells (ECs) and CD45−/Ter119−/CD44−/PDGFR+ mesenchymal stromal cells (MSCs) were determined
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DNA damage-triggered activation of cGAS-STING pathway induces apoptosis in human keratinocyte HaCaT cells Mol. Immunol. (IF 3.641) Pub Date : 2021-01-08 Can Li; Weiwei Liu; Fang Wang; Toshihiko Hayashi; Kazunori Mizuno; Shunji Hattori; Hitomi Fujisaki; Takashi Ikejima
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IgE and IgG4 epitopes revealed on the major fish allergen Lat c 1 Mol. Immunol. (IF 3.641) Pub Date : 2021-01-08 Michael F. Sharp; Aya C. Taki; Thimo Ruethers; Juan N. Stephen; Norelle L. Daly; Andreas L. Lopata; Sandip D. Kamath
Background The IgE- and IgG4-binding patterns of the major fish allergen parvalbumins are not clearly understood. IgE antibody-binding to parvalbumin from Asian seabass, Lat c 1.01, is implicated in up to 90 % of allergic reactions, although the region of IgE or IgG4 epitopes are unknown. In the present study, we characterized the specific IgE- and IgG4-binding regions of Lat c 1.01 using serum from
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Mycobacterium tuberculosis EsxL induces TNF-α secretion through activation of TLR2 dependent MAPK and NF-κB pathways Mol. Immunol. (IF 3.641) Pub Date : 2021-01-06 Kali Prasad Pattanaik; Geetanjali Ganguli; Sumanta Kumar Naik; Avinash Sonawane
Mycobacterium tuberculosis (Mtb) employs distinct strategies to circumvent host immune responses during the infection process. Various Mtb cell-wall associated and secretory proteins are known to play a critical role in the orchestration of host innate immune responses through modulation of signaling pathways. Mtb genome encodes for 23 (EsxA-EsxW) proteins belonging to the ESAT-6 like family; however
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The role of the tyrosine kinase Lyn in allergy and cancer Mol. Immunol. (IF 3.641) Pub Date : 2021-01-06 Yizhao Sun; Yanlei Yang; Yang Zhao; Xiangsheng Li; Yanfen Zhang; Zhongcheng Liu
With worsening air pollution brought by global social development, the prevalence of allergic diseases has increased dramatically in the past few decades. The novel Lck/yes-related protein tyrosine kinase (Lyn) belongs to the Src kinase family (SFK) and plays a pivotal role in the pathogenesis of inflammation, tumor, and allergy. This signaling molecule is vital in the IgE/FcεRI signaling pathway that
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Sticholysins, pore-forming proteins from a marine anemone can induce maturation of dendritic cells through a TLR4 dependent-pathway Mol. Immunol. (IF 3.641) Pub Date : 2021-01-06 Rady J. Laborde; Mayari E. Ishimura; Lianne Abreu-Butin; Catarina V. Nogueira; Daniel Grubaugh; Yoelys Cruz-Leal; María C. Luzardo; Audry Fernández; Circe Mesa; Fabiola Pazos; Carlos Álvarez; María E. Alonso; Michael N. Starnbach; Darren E. Higgins; Luis E. Fernández; Ieda M. Longo-Maugéri; María E. Lanio
Sticholysins (Sts) I and II (StI and StII) are pore-forming proteins (PFPs), purified from the Caribbean Sea anemone Stichodactyla helianthus. StII encapsulated into liposomes induces a robust antigen-specific cytotoxic CD8+ T lymphocytes (CTL) response and in its free form the maturation of bone marrow-derived dendritic cells (BM-DCs). It is probable that the latter is partially supporting in part
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Microarray gene expression profiling provides insights into functions of TIPE2 in HBV-related apoptosis Mol. Immunol. (IF 3.641) Pub Date : 2021-01-06 Jian Cui; Na Zhang; Ying Liu; Lei Zhang; Chengjiang Gao; Suxia Liu
Tumor necrosis factor-α-induced protein-8 like-2 (TNFAIP8L2, TIPE2), a member of TNFAIP8 family, functions as a regulator in inflammation. Our previous studies showed that TIPE2 can negatively regulate HBV-specific CD8+ T lymphocyte functions. But the effect of TIPE2 on the apoptosis of HBV-infected hepatocytes which is very important for eliminating viruses remains unclear. Using gene expression microarray
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Integrative analysis identifies potential causal methylation-mRNA regulation chains for rheumatoid arthritis Mol. Immunol. (IF 3.641) Pub Date : 2020-12-30 Xing-Bo Mo; Yong-Hong Zhang; Shu-Feng Lei
Genome-wide association studies have identified many genetic loci for rheumatoid arthritis (RA). However, causal factors underlying these loci were largely unknown. The aim of this study was to identify potential causal methylation-mRNA regulation chains for RA. We identified differentially expressed mRNAs and methylations and conducted summary statistic data-based Mendelian randomization (SMR) analysis
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Molecular interactions of the CTLA-4 cytoplasmic region with the phosphoinositide 3-kinase SH2 domains Mol. Immunol. (IF 3.641) Pub Date : 2020-12-30 Momoka Iiyama; Nobutaka Numoto; Shuhei Ogawa; Masataka Kuroda; Hisayuki Morii; Ryo Abe; Nobutoshi Ito; Masayuki Oda
During T-cell regulation, T-cell receptors and CD28 lead to signaling activation, while T-lymphocyte antigen 4 (CTLA-4) is known to lead to downregulation, similar to programmed cell death-1 (PD-1). In the cytoplasmic tails of CD28 and CTLA-4, phosphoinositide 3-kinase (PI3K) binds to the consensus sequence including phosphotyrosine via SH2 domains, N- and C-terminal SH2 domains (nSH2 and cSH2), of
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Animal models for human group 1 CD1 protein function Mol. Immunol. (IF 3.641) Pub Date : 2020-12-29 Emmelie Eckhardt; Max Bastian
The CD1 antigen presenting system is evolutionary conserved and found in mammals, birds and reptiles. Humans express five isoforms, of which CD1a, CD1b and CD1c represent the group 1 CD1-molecules. They are recognized by T cells that express diverse αβ-T cell receptors. Investigation of the role of group 1 CD1 function has been hampered by the fact that CD1a, CD1b and CD1c are not expressed by mice
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A hemocyte-specific cathepsin L-like cysteine protease is involved in response to 20-hydroxyecdysone and microbial pathogens stimulation in silkworm, Bombyx mori Mol. Immunol. (IF 3.641) Pub Date : 2020-12-26 Guangzhao Pan; Kui Zhang; Chongyang Li; Xin Hu; Saima Kausar; Hongyu Gu; Liqun Yang; Hongjuan Cui
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Role of MAIT cells in metabolic diseases Mol. Immunol. (IF 3.641) Pub Date : 2020-12-25 Amine Toubal; Agnès Lehuen
MAIT cells are innate-like T cells that are enriched in mucosal sites and tissues including adipose tissue and liver. They play an important role in immunity against microbial pathogens. Recently, it has been reported that MAIT cells could also be important in metabolic diseases and can be involved in setting up and maintaining chronic inflammation. In this review, we give an overview of recent advances
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DZNep attenuates allergic airway inflammation in an ovalbumin-induced murine model Mol. Immunol. (IF 3.641) Pub Date : 2020-12-25 Hang Li; Jian Li; Tong Lu; Dehua Chen; Rui Xu; Wei Sun; Xi Luo; Huabin Li; Renqiang Ma; Weiping Wen
Background Growing evidence shows that enhancer of zeste homolog 2 (EZH2) plays a role in various physiological functions and cancer pathogenesis. However, its contribution to allergic diseases remains controversial. We sought to investigate the role of EZH2 in the pathogenesis of allergic airway inflammation. Methods 3-Deazaneplanocin A (DZNep), an indirect inhibitor of EZH2, was administered via
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Identification of cell-surface glycans that mediate motility-dependent binding and internalization of Pseudomonas aeruginosa by phagocytes Mol. Immunol. (IF 3.641) Pub Date : 2020-12-25 Hector Sanchez; Daniel Hopkins; Sally Demirdjian; Cecilia Gutierrez; George A. O’Toole; Sriram Neelamegham; Brent Berwin
Phagocytic cells are critical to host defense against Pseudomonas aeruginosa, a Gram-negative bacterium that is an opportunistic pathogen. Accordingly, susceptible individuals frequently have impaired innate immune responses, including those with cystic fibrosis or neutropenia. Previous studies identified that the downregulation, or loss, of bacterial flagellar motility enables bacteria to evade interactions
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Biological functions of MAIT cells in tissues Mol. Immunol. (IF 3.641) Pub Date : 2020-12-25 Paul Klenerman; Timothy S.C. Hinks; James E. Ussher
Mucosal associated invariant T (MAIT) cells have a recognised innate-like capacity for antibacterial host defence, consequent on the specificity of their T cell receptor (TCR) for small molecule metabolites produced by a range of prokaryotic and fungal species, their effector memory phenotype, and their expression of cytotoxic molecules. However, recent studies have identified at least two other important
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Antigen specificities and functional properties of MR1-restricted T cells Mol. Immunol. (IF 3.641) Pub Date : 2020-12-25 Gennaro De Libero; Andrew Chancellor; Lucia Mori
MR1 is an MHC class I-like molecule with unique structural and biological features that make it an important member among the molecules involved in antigen presentation to T cells. Distinctive features include ubiquitous expression of the MR1 gene and its monomorphism. Another relevant property is that the MR1 protein appears at very low levels on the plasma membrane and its surface expression is regulated
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T cell receptor diversity, specificity and promiscuity of functionally heterogeneous human MR1-restricted T cells Mol. Immunol. (IF 3.641) Pub Date : 2020-12-23 Marco Lepore; Deborah A. Lewinsohn; David M. Lewinsohn
The monomorphic MHC-class I-like molecule, MR1, presents small metabolites to T cells. MR1 is the restriction element for microbe-reactive mucosal-associated invariant T (MAIT) cells. MAIT cells have limited TCR usage, including a semi-invariant TCR alpha chain and express high levels of CD161 and CD26. In addition to microbial lumazine metabolites, recent studies have demonstrated that MR1 is able
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USP14 negatively regulates RIG-I-mediated IL-6 and TNF-α production by inhibiting NF-κB activation Mol. Immunol. (IF 3.641) Pub Date : 2020-12-23 Hongrui Li; Jiazheng Quan; Xibao Zhao; Jing Ling; Weilin Chen
Ubiquitin specific protease 14 (USP14) is a regulator of protein deubiquitination and proteasome activation, and has been implicated in negative regulation of type I IFN signaling pathway. However, the effect of USP14 on RNA virus-related inflammatory response has not been studied. Retinoic acid-inducible gene I (RIG-I) is the important pattern recognition receptor of the innate immunity to detect
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Stenotrophomonas-maltophilia inhibits host cellular immunity by activating PD-1/PD-L1 signaling pathway to induce T-cell exhaustion Mol. Immunol. (IF 3.641) Pub Date : 2020-12-21 Min Wang; Sheng Yin; Qi Qin; Yizhi Peng; Zhengang Hu; Xiaolin Zhu; Lei Liu; Xianping Li
Background Smalotrophomonas maltophilia(S. maltophilia) is common in nosocomial infections. However, few studies have revealed the effect of S. maltophilia on cellular immunity in the host's immune system up to now. In clinical work, we accidentally discovered that S. maltophilia directly stimulated T cells to secrete IFN-γ. Materials and methods S. maltophilia was co-cultured with PBMCs to detect
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Reading the room: iNKT cells influence B cell responses Mol. Immunol. (IF 3.641) Pub Date : 2020-12-22 Elizabeth A. Leadbetter; Mikael C.I. Karlsson
Rapid immune responses regulated by invariant Natural Killer T (iNKT) cells bridge the gap between innate and adaptive responses to pathogens, while also providing key regulation to maintain immune homeostasis. iNKT immune protection and immune regulation are both mediated through interactions with innate and adaptive B cell populations that express CD1d. Recent studies have expanded our understanding
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Mouse models illuminate MAIT cell biology Mol. Immunol. (IF 3.641) Pub Date : 2020-12-22 Huimeng Wang; Zhenjun Chen; James McCluskey; Alexandra J Corbett
The field of mucosal-associated invariant T cell (MAIT) biology has grown rapidly since the identification of the vitamin-B-based antigens recognised by these specialised T cells. Over the past few years, our understanding of the complexities of MAIT cell function has developed, as they find their place among the other better known cells of the immune system. Key questions relate to understanding when
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Curcumin and inflammatory bowel diseases: From in vitro studies to clinical trials Mol. Immunol. (IF 3.641) Pub Date : 2020-12-19 Farzaneh Fallahi; Sarina Borran; Milad Ashrafizadeh; Ali Zarrabi; Mohammad Hossein Pourhanifeh; Mahmood Khaksary Mahabady; Amirhossein Sahebkar; Hamed Mirzaei
Inflammatory bowel diseases (IBDs) may result from mutations in genes encoding for innate immunity, which can lead to exacerbated inflammatory response. Although some mono-targeted treatments have developed in recent years, IBDs are caused through several pathway perturbations. Therefore, targeting all these pathways is difficult to be achieved by a single agent. Moreover, those mono-targeted therapies
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MAIT cell development in mice and humans Mol. Immunol. (IF 3.641) Pub Date : 2020-12-19 Marion Salou; François Legoux; Olivier Lantz
MAIT cells arise in the thymus following rearrangement of a T cell receptor (TCR) reactive against microbial vitamin B2-derived metabolites presented by the MHC-Ib molecule, MR1. Mechanisms that are conserved in mammals ensure the frequent production of MR1-restricted TCRs and the intra-thymic differentiation of MR1-restricted thymocytes into effector cells. Upon thymic egress and migration into non-lymphoid
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CD1a function in human skin disease Mol. Immunol. (IF 3.641) Pub Date : 2020-12-18 Annemieke de Jong; Graham Ogg
The high expression of CD1a on Langerhans cells in normal human skin suggests a central role for this lipid antigen presenting molecule in skin homeostasis and immunity. Although the lipid antigen presenting function of CD1a has been known for years, the physiological and pathological functions of the CD1a system in human skin remain incompletely understood. This review provides an overview of this
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Using agonists for iNKT cells in cancer therapy Mol. Immunol. (IF 3.641) Pub Date : 2020-12-16 Gavin F. Painter; Olivia K. Burn; Ian F. Hermans
The capacity of α-galactosylceramide (α-GalCer) to act as an anti-cancer agent in mice through the specific stimulation of type I NKT (iNKT) cells has prompted extensive investigation to translate this finding to the clinic. However, low frequencies of iNKT cells in cancer patients and their hypo-responsiveness to repeated stimulation have been seen as barriers to its efficacy. Currently the most promising
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Enhanced antiviral benefit of combination therapy with anti-HBV and anti-PD1 gRNA/cas9 produces a synergistic antiviral effect in HBV infection Mol. Immunol. (IF 3.641) Pub Date : 2020-12-16 Shuai Zhen; Rong Qiang; Jiaojiao Lu; Xiaoqian Tuo; Xiling Yang; Xu Li
Targeted therapy for patients with hepatitis B virus (HBV) infection can lead to objective responses, although response times may be short. At the same time, the response rate to programmed cell death-1 (PD-1) treatment was more durable. It is speculated that HBV targeted therapy can synergistically enhance the antitumor activity with PD-1 blockade. To test this hypothesis, we evaluated the effect
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3,3'-Diindolylmethane alleviates acute atopic dermatitis by regulating T cell differentiation in a mouse model Mol. Immunol. (IF 3.641) Pub Date : 2020-12-11 Xianxian Wu; Jinxuan Liu; Chaoqin Chen; Zhen Huang; Yuhui Zang; Jiangning Chen; Lei Dong; Junfeng Zhang; Zhi Ding
Atopic dermatitis is a severe, chronic relapsing inflammatory disease of the skin with family clustering. It is characterized into acute phase, which is dominated by T helper 2-type immune responses, and chronic phase, which is dominated by T helper 1-type immune responses. Studies have shown that 3,3′-diindolylmethane not only has antitumor effects but also can relieve symptoms of inflammatory diseases
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Astragaloside IV antagonizes M2 phenotype macrophage polarization-evoked ovarian cancer cell malignant progression by suppressing the HMGB1–TLR4 axis Mol. Immunol. (IF 3.641) Pub Date : 2020-12-09 Xue Wang; ShouYang Gao; LiYou Song; Ming Liu; ZiQian Sun; JunBao Liu
Macrophages are the most abundant cells in tumor stroma and their polarization within tumor microenvironment exert the key roles in tumorigenesis. Astragaloside IV is a natural extract from traditional Chinese herbal Radix Astragali, and fulfills pleiotropic function in several cancers. Nevertheless, its function in ovarian cancer microenvironment remains elusive. In the present research, astragaloside
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Asiaticoside ameliorates acinar cell necrosis in acute pancreatitis via toll-like receptor 4 pathway Mol. Immunol. (IF 3.641) Pub Date : 2020-12-09 Keyan Wu; Guanghuai Yao; Xiaolei Shi; Huan Zhang; Qingtian Zhu; Xinnong Liu; Guotao Lu; Lianghao Hu; Weijuan Gong; Qi Yang; Yanbing Ding
Acinar cell necrosis is one of the most prominent pathophysiological changes of acute pancreatitis (AP). Asiaticoside (AS) is a triterpene compound with confirmed apoptosis-and necrosis-related activities. However, the specific effects of AS on AP have not been determined. In this study, we aimed to investigate the protective effect of AS on AP using two mouse models. In the caerulein-induced mild
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Understanding and modulating the MR1 metabolite antigen presentation pathway Mol. Immunol. (IF 3.641) Pub Date : 2020-12-06 Hamish E.G. McWilliam; Mariolina Salio
The MHC class I-related protein, MR1, presents small metabolite antigens to an unusual subset of innate-like T cells. Herein, we highlight recent progress in our understanding of MR1’s unique antigen presenting pathway, with features of both MHC class I and class II antigen presentation, as highlighted during the EMBO Workshop: CD1-MR1, Beyond MHC-restricted lymphocytes, Oxford, 2019. There is increasing
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Chemical insights into the search for MAIT cells activators Mol. Immunol. (IF 3.641) Pub Date : 2020-12-06 Natacha Veerapen; Judith Hobrath; Amareeta K. Besra; Gurdyal S. Besra
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microRNA-30e up-regulation alleviates myocardial ischemia-reperfusion injury and promotes ventricular remodeling via SOX9 repression Mol. Immunol. (IF 3.641) Pub Date : 2020-12-05 Nan Cheng; Libin Li; Yuanbin Wu; Mingyan Wang; Ming Yang; Shixiong Wei; Rong Wang
Aim At present, studies have focused on microRNAs (miRNAs) in myocardial ischemia-reperfusion injury (MI/RI). But the specific role of miR-30e hasn’t been fully explored. Thus, this study is to uncover the mechanism of miR-30e in MI/RI. Methods MI/RI models of rats and hypoxia/reoxygenation injury (H/R) models of H9C2 cardiomyocytes were established. Rats were injected with miR-30e and SRY-related
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Human DUBs’ gene expression and regulation in antiviral signaling in response to poly (I:C) treatment Mol. Immunol. (IF 3.641) Pub Date : 2020-12-02 Kunpeng Liu; Dongbo Qiu; Xue Liang; Yingqi Huang; Jingyuan Zhao; Xiusheng Qiu; Qi Zhang; Zhen-Dong Xiao; Yunfei Qin
Type I interferons (IFNs) play a central role in host defense against viral infection. Multiple posttranslational modifications including ubiquitination and deubiquitination regulate the function of diverse molecules in type I IFN signaling. Many ubiquitin ligase enzymes, such as those of the TRAF and TRIM families, have been shown to participate in the production of type I IFNs and inflammatory cytokines
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A novel BAFF antagonist, BAFF-Trap, effectively alleviates the disease progression of systemic lupus erythematosus in MRL/lpr mice Mol. Immunol. (IF 3.641) Pub Date : 2020-11-27 Chaoheng Yu; Shuang Chen; Bailing Zhou; Hailong Zhang; Xiaoqing Su; Yi Luo; Li Yang
Abnormal B cells, which produce antibodies against self-antigens, play a key role in the pathogenesis of autoimmune diseases, such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). B-cell activating factor (BAFF) is closely associated with abnormal B cells and participates in B cell-mediated autoimmune diseases; thus, neutralizing BAFF is an effective method for treating these diseases
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Molecular characterization and antiparasitic activity analysis of a novel piscidin 5-like type 4 from Larimichthys crocea Mol. Immunol. (IF 3.641) Pub Date : 2020-11-27 Libing Zheng; Jiayin Qiu; Huihui Liu; Huilai Shi; Changfeng Chi; Ying Pan
Cryptocaryon irritans is an obligate parasitic ciliate protozoan that can infect various commercially important mariculture teleosts and cause high lethality and economic loss, especially Larimichthys crocea. Current methods of controlling or preventing this parasite with chemicals or antibiotics are widely considered to be environmentally harmful. The antiparasitic activity of some antimicrobial peptides
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Antigen presentation genes in gadoid species (haddock: Melanogrammus aeglefinus and Atlantic cod: Gadus morhua) raise questions about cross-presentation pathways and glycosylated beta-2-microglobulin Mol. Immunol. (IF 3.641) Pub Date : 2020-11-28 Aaron Frenette; Marije Booman; Kazuhiro Fujiki; Stephen Kales; Collen Ryan; A. Kurt Gamperl; Brian Dixon
The Atlantic cod immune system deviates from antigen presentation processes seen in other vertebrates in that it lacks the necessary genes for exogenous antigen presentation (i.e., MHC-II and li) and a key MHC-II interacting molecule necessary for T-helper cell function (i.e., CD4), while possessing an expanded repertoire of MHC-I genes that facilitate endogenous antigen presentation. These observations
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Tryptanthrin suppresses double-stranded RNA-induced CXCL10 expression via inhibiting the phosphorylation of STAT1 in human umbilical vein endothelial cells Mol. Immunol. (IF 3.641) Pub Date : 2020-11-28 Shogo Kawaguchi; Hirotake Sakuraba; Hidezumi Kikuchi; Noriyuki Numao; Taka Asari; Hiroto Hiraga; Jiangli Ding; Tomoh Matsumiya; Kazuhiko Seya; Shinsaku Fukuda; Tadaatsu Imaizumi
Tryptanthrin is a bioactive component of indigo plants such as Polygonum tinctrorium and known to have an anti-inflammatory activity. The aim of this study was to investigate the effects of tryptanthrin on Toll-like receptor 3 (TLR3)-mediated cytokine and chemokine expression in human umbilical vein endothelial cells (HUVEC). Herein, we found that tryptanthrin suppressed the expression of CXCL10 in
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Immunomodulatory gene expression analysis in LPS-stimulated human polymorphonuclear leukocytes treated with antibiotics commonly used for multidrug-resistant strains Mol. Immunol. (IF 3.641) Pub Date : 2020-11-30 Tsuneyuki Ubagai; Yoshinori Sato; Go Kamoshida; Yuka Unno; Yasuo Ono
Conventional antibiotics used for the treatment of severe infections such as sepsis and septic shock confer immunomodulatory benefits. However, the growing problem of multidrug resistant infections has led to an increase in the administration of non-conventional last-resort antibiotics, including quinolones, aminoglycosides, and polypeptides, and the effects of these drugs on immunomodulatory gene
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Impact of knockdown LincRNA-Cox2 on apoptosis of macrophage infected with Bacillus Calmette-Guérin Mol. Immunol. (IF 3.641) Pub Date : 2020-11-26 Yanan Xu; Jialin Yu; Chenjie Ma; Zhaoqian Gong; Xiaoling Wu; Guangcun Deng
Mycobacterium tuberculosis (Mtb)-induced apoptosis of alveolar macrophages plays an important role in the pathogenesis of tuberculosis. Previous studies indicated that massive LncRNAs could deteriorate MTB invasion or latent infection by regulating macrophage’s apoptosis. However, whether LincRNA-Cox2 is involved in apoptosis of macrophage infected with Mtb is unclear. In this study, we found Bacillus
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Identification of key genes involved in JAK/STAT pathway in colorectal cancer Mol. Immunol. (IF 3.641) Pub Date : 2020-11-25 Yuanyi Yue; Qiang Zhang; Si Wu; Shuang Wang; Changwan Cui; Miao Yu; Zhengrong Sun
JAK/STAT pathway has been well confirmed in the development of colorectal cancer (CRC), however, the exact mechanism is unclear. Therefore, we aimed to identify key genes involved in JAK/STAT pathway in CRC, as well as the potential mechanism. RT² profiler PCR arrays were performed to identify key genes of the JAK/STAT pathway. GO, KEGG pathway and PPI analyses were performed to screen the main functions
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Memory T cells of patients with abdominal aortic aneurysm differentially expressed micro RNAs 21, 92a, 146a, 155, 326 and 663 in response to Helicobacter pylori and Lactobacillus acidophilus Mol. Immunol. (IF 3.641) Pub Date : 2020-11-24 Mehdi Kalani; Hossein Hodjati; Hamed Ghoddusi Johari; Mehrnoosh Doroudchi
Regarding the role of micro RNAs (miRNA) in the proliferation and differentiation of T cells as well as the controversy around the role of bacteria in the pathogenesis of abdominal aortic aneurysm (AAA), the effects of Helicobacter pylori (Hp) and Lactobacillus acidophilus (La) were investigated in the induction of miRNAs and apoptosis in CD4+ memory T (Tem) cells of AAA patients and controls. Signature
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Transcriptome sequencing reveals Cnaphalocrocis medinalis against baculovirus infection by oxidative stress Mol. Immunol. (IF 3.641) Pub Date : 2020-11-20 Guangjie Han; Qin Liu; Chuanming Li; Bin Xu; Jian Xu
Cnaphalocrocis medinalis granulovirus (CnmeGV) is a potential microbial agent against the rice leaffolder. Innate immunity is essential for insects to survive pathogenic infection. Therefore, to clarify the immune response of Cnaphalocrocis medinalis to the viral colonization, the gene expression profile of C. medinalis infected with CnmeGV was constructed by RNA-seq. A total of 8,503 differentially
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HtrA2 is required for inflammatory responses in BMDMs via controlling TRAF2 stability in collagen-induced arthritis Mol. Immunol. (IF 3.641) Pub Date : 2020-11-20 Zhitong Xu; Jinding Lin; Yongsong Xie; Haifeng Tang; Junjie Xie; Rongdong Zeng
Rheumatoid arthritis (RA) is an autoimmune inflammatory disease characterized by the destruction of cartilage and bone. The present study aims to investigate the role of HtrA serine peptidase 2 (HtrA2) in the collagen-induced arthritis. The expressions of HtrA2 were determined in the database BioGPS and bone marrow-derived macrophages (BMDMs). The populations of myeloid and lymphoid cells were determined
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Specific Ag-guiding nano-vaccines attenuate neutrophil-dominant allergic asthma Mol. Immunol. (IF 3.641) Pub Date : 2020-11-20 Mei-Zhen Zhao; Yan Li; Hai-Yang Han; Li-Hua Mo; Gui Yang; Zhi-Qiang Liu; Chang Ma; Ping-Chang Yang; Shuwen Liu
Polymorphonuclear neutrophils (PMN) are one fraction of the major inflammatory cells in allergic asthma (asthma, in short); the role of PMN in the asthma pathogenesis is not fully understood yet. This study aims to investigate the effects of specific Ag-guiding exosomes on suppressing the neutrophil-dominant airway inflammation. In this study, BALB/c mice were immunized with ovalbumin plus complete
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CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protection Mol. Immunol. (IF 3.641) Pub Date : 2020-11-19 Jian Cheng; Zhao Liu; Tian Deng; Zhen Lu; Maoxuan Liu; Xiaoxu Lu; Funmilayo Oladunni Adeshakin; Dehong Yan; Guizhong Zhang; Xiaochun Wan
Immune evasion is a common hallmark of cancers. Immunotherapies that aim at restoring or increasing the immune response against cancers have revolutionized outcomes for patients, but the mechanisms of resistance remain poorly defined. Here, we report that CD317, a surface molecule with a unique topology that is double anchored into the membrane, protects tumor cells from immunocytolysis. CD317 knockdown
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Enhanced RIPK3 kinase activity-dependent lytic cell death in M1 but not M2 macrophages Mol. Immunol. (IF 3.641) Pub Date : 2020-11-18 Qin Hao; Suman Kundu; Joshua Kleam; Zhizhuang Joe Zhao; Steven Idell; Hua Tang
Macrophages play a crucial role in host innate immune defense against infection and tissue injury. Macrophages are highly plastic cells and their subtypes have been characterized as M1 (also termed classically activated) and M2 (alternatively activated). Although the M1/M2 paradigm has been well documented, less is known regarding the role of macrophage activation/polarization in inflammation-associated
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