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Black soldier fly (Hermetia illucens) larvae meal in diets of European seabass: Effects on antioxidative capacity, non-specific immunity, transcriptomic responses, and resistance to the challenge with Vibrio alginolyticus Fish Shellfish Immun. Pub Date : 2021-01-25 Hany M.R. Abdel-Latif; Mohsen Abdel-Tawwab; Riad H. Khalil; Ahmed A. Metwally; Medhat S. Shakweer; Hanan A. Ghetas; Mohamed A. Khallaf
Black soldier fly larvae meal (BSFLM) has been successfully demonstrated as a promising fish meal (FM) replacer in diets of several fish species including European seabass (Dicentrarchus labrax). However, its impacts on antioxidant capacity, and immune responses of treated fish are still poorly understood. A 60-day feeding trial was carried out to evaluate the effects of partial substitution of FM
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Molecular characterization, expression analysis and immune effect of Galectin-8 from Japanese flounder (Paralichthys olivaceus) Fish Shellfish Immun. Pub Date : 2021-01-24 Zhixiao Dong; Yingrui Li; Xiaofei Liu; Lin Su; Shun Zhou; Yunji Xiu
Galectin-8 gene belongs to the agglutinin family, which can specifically recognize β-galactoside bonds and play essential roles in many biological processes. In this study, we researched the sequence characteristics and immune-related function of Galectin-8 gene in Japanese flounder Paralichthys olivaceus, named PoGalectin-8. The results showed that the open reading frame of PoGalectin-8 was 891 bp
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Toxic effects of dietary copper and EGCG on bioaccumulation, antioxidant enzyme and immune response of Korean bullhead, Pseudobagrus fulvidraco Fish Shellfish Immun. Pub Date : 2021-01-24 Huisu Lee; Jun-Hwan Kim; Hee-Ju Park; Ju-Chan Kang
There are few reports of dietary Cu (copper) toxicity to Korean bullhead, Pseudobagrus fulvidraco, and little is known about recovery from dietary Cu exposure. In this study, P. fulvidraco (mean length 16.9 ± 1.38 cm, and mean weight 53.2 ± 1.22 g) were exposed for 4 weeks to dietary Cu concentration of 0 (control), 700, 900, and 1,100 mg Cu kg-1 dry feed to establish maximum tolerable levels of dietary
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A standardized protocol for measuring phenoloxidase in captive and wild Murray crayfish Euastacus armatus Fish Shellfish Immun. Pub Date : 2021-01-24 Franz D. Scherping; Maggie J. Watson
Innate immunity in arthropods is achieved largely through melanization which is in turn the result of the prophenoloxidase (ProPO) activation cascade; a series of biochemical reactions triggered by the immune identification of pathogen-recognition proteins (PRPs). Within this activation cascade, inactive proPO is cleaved to form the reactive enzyme phenoloxidase (PO). Methods of detecting PO are used
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Proteomic analysis of body wall and coelomic fluid in Sipunculus nudus Fish Shellfish Immun. Pub Date : 2021-01-15 Yupo Cao; Xuli Lu; Yaping Dai; Yahui Li; Fei Liu; Wei Zhou; Jihua Li; Baodong Zheng
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Immunity and inflammatory responses in gilthead sea bream (Sparus aurata L.) exposed to sub-lethal mixture of carbamazepine, cadmium chloride and polybrominated diphenyl ether Fish Shellfish Immun. Pub Date : 2020-12-23 Cristóbal Espinosa-Ruiz; Simona Manuguerra; Maria Morghese; José María García-Beltrán; María Ángeles Esteban; Marta Giuga; Concetta M. Messina; Andrea Santulli
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Transcriptome analysis of immune- and iron-related genes after Francisella noatunensis subsp. orientalis infection in Nile tilapia (Oreochromis niloticus) Fish Shellfish Immun. Pub Date : 2021-01-11 Shun Maekawa; Theeraporn Pulpipat; Pei-Chi Wang; Shih-Chu Chen
Francisella noatunensis subsp. orientalis (Fno) is a gram-negative intracellular bacterium identified in many fish species worldwide, including cultured Nile tilapia (Oreochromis niloticus) in Taiwan. To investigate the gene expression responses to Fno infection, we performed transcriptome analysis of the head kidney and spleen in Nile tilapia using RNA-seq. Total RNA was extracted from the head kidney
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Transcriptome-wide identification and characterization of Toll-like receptors response to Vibrio anguillarum infection in Manila clam (Ruditapes philippinarum) Fish Shellfish Immun. Pub Date : 2021-01-22 Yipeng Ren; Huaxi Liu; Siying Fu; Wenhao Dong; Baoping Pan; Wenjun Bu
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Identification, expression and pro-inflammatory effect of interleukin-17 N in common carp (Cyprinus carpio L.) Fish Shellfish Immun. Pub Date : 2020-12-30 Hongxia Li; Lei Zhang; Jianlin Li; Fan Yu; Meiyao Wang; Qin Wang; Yunsheng Wu; Qiyuan Zhang; Yongkai Tang; Juhua Yu
Two interleukin (IL)-17 N genes (CcIL-17Na and b) present on different linkage groups were identified in the common carp (Cyprinus carpio) genome and confirmed by polymerase chain reaction (PCR) and real time (RT)-PCR in this experiment. Synteny analysis revealed that IL-17 N is transcribed by the complement sequence of TOP3B's intron 2. It is flanked by SDF2L and PPM1F in all fish studied to date
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Effect of miR-155 on type I interferon response in Epithelioma papulosum cyprini cells Fish Shellfish Immun. Pub Date : 2021-01-15 Jun Soung Kwak; Ki Hong Kim
MicroRNA-155 (miRNA-155) is known to play an important role in the regulation of innate and adaptive immune responses in mammals. However, no information is available on the role of miRNA-155 in relation to type I interferon (IFN) responses in fish cells. In the present study, we found that the protein inhibitor of activated STAT 4a (PIAS4a) gene of fathead minnow (Pimephales promelas) was a target
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Cerocin, a novel piscidin-like antimicrobial peptide from black seabass, Centropristis striata Fish Shellfish Immun. Pub Date : 2020-12-24 Ying Qiao; Xiaowan Ma; Man Zhang; Shengping Zhong
Antimicrobial peptides, which are crucial effectors of innate immunity, are a promising substitute for antibiotics. The piscidin family is a group of fish-derived antimicrobial peptides that have potent antimicrobial activity and participate in the innate immune response. Here we describe a novel piscidin-like peptide called cerocin from the black sea bass (Centropristis striata), which is a highly
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Recognition of DAP and activation of NF-κB by cytosolic sensor NOD1 in Oreochromis niloticus Fish Shellfish Immun. Pub Date : 2021-01-12 Jianan He; Zining Meng; Danqi Lu; Xiaochun Liu; Haoran Lin
As a lower vertebrate, the immune defense mechanism of fish mainly depends on the innate immune system. Nucleotide-binding oligomerization domain-like receptors (NLRs) are an important class of pattern recognition receptors in the innate immune system. In this study, NOD1 gene was cloned and characterized in Nile tilapia (Oreochromis niloticus). The ORF of Nile tilapia NOD1 gene was 2826 bp long and
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Dual effect of Selenium loaded Chitosan Nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus) Fish Shellfish Immun. Pub Date : 2021-01-14 Doaa Ibrahim; Ahmed N. Attyia Nem; Seham.M. Ibrahim; Hemmat M. Eissa; M.M. Fawzey; Doaa I.A. Mostafa; Shaimaa.A.Abd El-Kader; S.I. Khater; Safaa I. Khater
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Cadmium exposure induces inflammation through the canonical NF-κΒ pathway in monocytes/macrophages of Channa punctatus Bloch Fish Shellfish Immun. Pub Date : 2021-01-13 Chohelee Choudhury; Ritwik Mazumder; Rajib Biswas; Mahuya Sengupta
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Chitinase involved in immune regulation by mediated the toll pathway of crustacea Procambarus clarkii Fish Shellfish Immun. Pub Date : 2020-12-28 Min Liu; Chen Chen; Qi-Cheng Wu; Jia-Le Chen; Li-Shang Dai; Sheng Hui Chu; Qiu-Ning Liu
Chitinase can degrade chitin and play an essential role in animal immunity and plant defense. The immune functions of Chitinase in Procambarus clarkii (P. clarkii) remain to elucidate. Here, we identified PcChitinase 2 gene sequence from P. clarkii and studied its spatial and temporal expression profiles. The PcChitinase 2 transcribed unequally in different tissues; however, its expression was highest
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Proteomic profile and protease activity in the skin mucus of greater amberjack (Seriola dumerili) infected with the ectoparasite Neobenedenia girellae — an immunological approach Fish Shellfish Immun. Pub Date : 2021-01-11 Álvaro Fernandez-Montero; Silvia Torrecillas; Daniel Montero; Felix Acosta; María-José Prieto-Álamo; Nieves Abril; Juan Jurado
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A novel herbal immunostimulant for rainbow trout (Oncorhynchus mykiss) against Yersinia ruckeri Fish Shellfish Immun. Pub Date : 2020-12-28 Ertugrul Terzi; Bunyamin Kucukkosker; Soner Bilen; Osman Nezih Kenanoglu; Orhan Corum; Mehmet Özbek; Sukru Senol Parug
In this 21-day study, we examined the effects of the aqueous methanolic extract of thin-skinned plum (Prunus domestica) on growth, immune response and resistance to a pathogenic bacterium, Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss). Fish were fed with diets containing thin-skinned plum extract doses as 0 (Control) 0.1 (PD01), 0.5 (PD05) and 1% (PD1) ad libitum twice in a day. At the end
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Type I interferons in ray-finned fish (Actinopterygii) Fish Shellfish Immun. Pub Date : 2020-12-31 Michał Stosik; Beata Tokarz-Deptuła; Wiesław Deptuła
Interferons (IFNs) are proteins of vital importance in the body's immune response. They are formed in different types of cells and have been found in fish, amphibians, reptiles and mammals. Two types of IFN have been found in ray-finned fish (Superclass: Osteichthyes, Class: Actinopterygii) so far, i.e. IFN type I (IFN I) and IFN type II (IFN II), while the presence of IFN type III (IFN III), which
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Octominin: An antibacterial and anti-biofilm peptide for controlling the multidrug resistance and pathogenic Streptococcus parauberis Fish Shellfish Immun. Pub Date : 2020-12-27 E.H.T. Thulshan Jayathilaka; T.D. Liyanage; D.C. Rajapaksha; S.H.S. Dananjaya; Chamilani Nikapitiya; Ilson Whang; Mahanama De Zoysa
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Monoclonal antibody against Nile tilapia (Oreochromis niloticus) IgM heavy chain: A valuable tool for detection and quantification of IgM and IgM+ cells Fish Shellfish Immun. Pub Date : 2020-12-18 Janet Velázquez; Alianet Rodríguez; Hasel Aragón; Arlette Haidar; Marcos González; Rodolfo Valdés; Hilda Elsa Garay; David Diago Abreu; Yassel Ramos; Ania Cabrales; Antonio Morales; Osmany González; Fidel Herrera; Mario Pablo Estrada; Yamila Carpio
Nile tilapia (Oreochromis niloticus) is a freshwater fish, which is extensively cultivated worldwide and constitutes one of the model species for the study of fish immunology. Monoclonal antibodies are very advantageous molecular tools for studying teleost immune system. Specifically, monoclonal antibodies that react with immunoglobulins are used successfully in the study of the humoral immune response
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Comparative study of coelomocytes from Arbacia lixula and Lythechinus variegatus: Cell characterization and in vivo evidence of the physiological function of vibratile cells Fish Shellfish Immun. Pub Date : 2020-12-27 Vinicius Queiroz; Sandra M. Muxel; Luigi Inguglia; Marco Chiaramonte; Márcio R. Custódio
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Immunopathogenesis of hematopoietic tissues in response to Vibrio parahaemolyticus (VPAHPND) infection in Macrobrachium rosenbergii Fish Shellfish Immun. Pub Date : 2020-12-29 Arnon Pudgerd; Thanapong Kruangkum; Kallaya Sritunyalucksana; Rapeepun Vanichviriyakit; Supapong Imsonpang; Charoonroj Chotwiwatthanakun
In crustacean, hemocytes are known as crucial components of crustaceans’ innate immunity against pathogens. Drastic hemocytes reduction during infectious disease is apparently related to disease severity and calls for a health status evaluation and aquaculture management. The molecular pathogenesis of hemocytes loss during bacterial infection was elucidated with VPAHPND challenged in M. rosenbergii
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Molecular characterization of the T cell costimulatory receptors CD28 and CTLA4 in the European sea bass Fish Shellfish Immun. Pub Date : 2020-12-18 Carmen González-Fernández; María A. Esteban; Alberto Cuesta
For the activation of T cells, it is necessary the specific recognition of the peptide by the T cell receptors (TCR) in the surface of antigen-presenting cells (APCs) and additional signals delivered by costimulatory receptors. In fish, knowledge about the presence of these costimulatory signals is limited and functional evidence almost absent. Thus, in this study, we have identified the stimulatory
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Pandanus tectorius fruit extract promotes Hsp70 accumulation, immune-related genes expression and Vibrio parahaemolyticus tolerance in the white-leg shrimp Penaeus vannamei Fish Shellfish Immun. Pub Date : 2020-12-24 Anupa Anirudhan; Victor Tosin Okomoda; Mat Taib Mimi Iryani; Yosie Andriani; Mohd Effendy Abd Wahid; Min Pau Tan; Muhd Danish-Daniel; Li Lian Wong; Tengku Sifzizul Tengku-Muhammad; Wen Jye Mok; Patrick Sorgeloos; Yeong Yik Sung
Plants and herbal extracts are indispensable for controlling the spread of disease-causing bacteria, including those that infect aquatic organisms used in aquaculture. The use of plant or herbal extract is expected to be safe for aquatic animals and less harmful to the environment, as opposed to conventional therapeutic alternatives such as antibiotics that promote the occurrence of potential antibiotic-resistant
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Characterization and functional analysis of fibrinogen-related protein (FreP) in the black tiger shrimp, Penaeus monodon Fish Shellfish Immun. Pub Date : 2020-12-23 Prawit Oangkhana; Piti Amparyup; Anchalee Tassanakajon; Elumalai Preetham; Ratree Wongpanya
Ficolin is classified as an immune related protein containing collagen-like and fibrinogen-related domain (FreD). In invertebrates, the functions of fibrinogen-related proteins (FrePs) are of importance to innate immunity. In this study, a FreP in the black tiger shrimp Penaeus monodon was identified and characterized. The PmFreP cDNA is 1,007 bp long with a 921 bp-open reading frame that encodes for
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Galectins in turbot (Scophthalmus maximus L.): Characterization and expression profiling in mucosal tissues Fish Shellfish Immun. Pub Date : 2020-12-13 Mengyu Tian; Dongxue Xu; Qiang Fu; Lu Zhang; Ning Yang; Ting Xue; Chengbin Gao; Qing Zhu; Yichao Ren; Min Cao; Fenghua Tan; Lin Song; Chao Li
Galectins, a family of evolutionary conserved β-galactoside-binding proteins, have been characterized in a wide range of species. Many reports have indicated vital roles of galectins in innate immunity, especially in the mucosal tissues against infection. However, the systematic identification of galectin gene family is still lacking in teleost. Here, we characterized the galectin gene family and investigated
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Genomic structure, expression and functional characterization of arginine kinase (EcAK) from Exopalaemon carinicauda Fish Shellfish Immun. Pub Date : 2020-12-19 Zixuan Wu; Yujie Liu; Jiaqi Zheng; Yongzhao Zhou; Kefan Xing; Yuying Sun; Jiquan Zhang
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Molecular characterization, immune and xenobiotic responses of glutathione S-transferase omega 1 from the big-belly seahorse: Novel insights into antiviral defense Fish Shellfish Immun. Pub Date : 2020-12-19 H.M.V. Udayantha; D.S. Liyanage; Kishanthini Nadarajapillai; W.K.M. Omeka; Hyerim Yang; Taehyug Jeong; Jehee Lee
Glutathione S-transferases (GSTs) are important enzymes involved in phase II detoxification and function by conjugating with the thiol group of glutathione. In this study, we isolated an omega class GST from the big-belly seahorse (Hippocampus abdominalis; HaGSTO1) to study the putative xenobiotic responses and defense ability against viral and bacterial infections in this animal. The isolated HaGSTO1
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Toposome: Source of antimicrobial molecules in the gonads of the sea urchin Lytechinus variegatus (Lamarck, 1816) Fish Shellfish Immun. Pub Date : 2020-12-01 DAL Figueiredo; WS Santos; Fabio Montoni; Leo Kei Iwai; PI Silva Junior
Sea urchins live in a challenging environment that requires rapid and efficient responses against pathogens and invaders. This response may be also important in reproductive processes once males and females release their gametes into water. In addition, the gonads are organs with dual function: reproductive organ and nutrient reserve, therefore it needs efficient protective mechanisms to preserve the
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Insect (Black soldier fly, Hermetia illucens) meal supplementation prevents the soybean meal-induced intestinal enteritis in rainbow trout and health benefits of using insect oil Fish Shellfish Immun. Pub Date : 2020-12-19 Vikas Kumar; Femi J. Fawole; Nicholas Romano; Md. Sakhawat Hossain; Shyam N. Labh; Ken Overturf; Brian C. Small
Black solider fly larvae (BSFL) and their oils (BSFLO) are receiving increasing attention as sustainable ingredients in fish feeds, but mostly as replacements to marine sources. There were two aims to this study; in exp. 1, soybean meal (SBM)-based diets were formulated to contain BSFL as supplements at 0 (SBM), 8 (SBM+BSFLlow) or 16% (SBM+BSFLhigh) with a control diet being fishmeal-based (FM). In
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Novel insights about the ascidian dopamine system: Pharmacology and phylogenetics of catecholaminergic receptors on the Phallusia nigra immune cells Fish Shellfish Immun. Pub Date : 2020-12-05 Jessica Fernandes de Souza; Andressa de Abreu Mello; Taynan Motta Portal; Rodrigo Nunes-da-Fonseca; Cintia Monteiro de Barros
Dopamine (DA) is an important molecule that plays a role in the nervous and immune systems. DA is produced by a wide variety of animals and it is considered one of the oldest neurotransmitters. However, its specific function in immune cells has not been completely revealed. In a group of chordate animals, the ascidians, DA is reported to be produced by cells in the central nervous system (CNS); however
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Protective effects of black seed (Nigella sativa) diet supplementation in common carp (Cyprinus carpio) against immune depression, oxidative stress and metabolism dysfunction induced by glyphosate Fish Shellfish Immun. Pub Date : 2020-12-04 Morteza Yousefi; Hossein Adineh; Miriam Reverter; Mohammad Khademi Hamidi; Yury Anatolyevich Vatnikov; Evgeny Vladimirovich Kulikov; Seyed Hossein Hoseinifar; Hien Van Doan
Sustainable aquaculture arises as key to increase food production in the coming years. However, the sector still faces many challenges such as the exposure of the cultured animals to pesticide-contaminated water. Pesticides used in agriculture can reach aquaculture systems either directly (integrated-agriculture aquaculture practices) or indirectly (soil leakage) and cause a broad range of ecotoxicological
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Dietary soy lecithin augments antioxidative defense and thermal tolerance but fails to modulate non-specific immune genes in endangered golden mahseer (Tor putitora) fry Fish Shellfish Immun. Pub Date : 2020-12-04 Alexander Ciji; M.S. Akhtar; Priyanka H. Tripathi; Anupam Pandey; Manchi Rajesh; Biju Sam Kamalam
A 70-day experiment was carried out to assess the effect of different levels (0, 1 and 2%) of soy lecithin in the diet on growth, survival, antioxidant defense markers, immune gene expression and thermal tolerance limits of golden mahseer, Tor putitora fry. Percentage weight gain, specific growth rate (SGR %) and survival of mahseer fed lecithin supplemented diets were not significantly different from
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Dietary taurine modulates hepatic oxidative status, ER stress and inflammation in juvenile turbot (Scophthalmus maximus L.) fed high carbohydrate diets Fish Shellfish Immun. Pub Date : 2020-12-05 Yue Zhang; Zehong Wei; Mengxi Yang; Danni Liu; Mingzhu Pan; Chenglong Wu; Wenbing Zhang; Kangsen Mai
This study was conducted to explore the beneficial role of taurine against chronic high carbohydrate diet-induced oxidative stress, endoplasmic reticulum (ER) stress and inflammation, and to understand the underlying molecular mechanisms in turbot. Two 10-week feeding trials were simultaneously conducted. For the one, six experimental diets with graded levels of taurine supplementation (0, 0.4%, 0
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Effects of dietary white mulberry leaves on hemato-biochemical alterations, immunosuppression and oxidative stress induced by Aeromonas hydrophila in Oreochromis niloticus Fish Shellfish Immun. Pub Date : 2020-12-08 Ahmed N.F. Neamat-Allah; Essam A. Mahmoud; Y. Mahsoub
The present work was designed to assess the potential hemato-biochemical protective action, immunemodulatory and antioxidant conclusions of varied concentration of white mulberry Morus alba leaves (MAL) extract supplementation on Nile tilapia (Oreochromis .niloticus). A total two hundred and forty of O. niloticus were haphazardly sorted into four groups. The control (CT) group was fed on basal diet
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Surviving under pollution stress: Antibacterial and antifungal activities of the Oyster species (Magallana bilineata and Magallana cuttackensis) Fish Shellfish Immun. Pub Date : 2020-11-30 Malik Wajid Hussain Chan; Khwaja Ali Hasan; Danilo Balthazar-Silva; Muhammad Asghar; Zulfiqar Ali Mirani
Antibiotic effectively controls the bacterial and fungal infections. Pathogens developing multi-drug resistance are a global health concern, which necessitate developing new molecules to overcome the resistance dilemma. This study explores the intrinsic ability of marine oysters synthesizing bioactive compounds. The tissue extracts prepared in n-hexane from two oysters, Magallana bilineata and Magallana
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Effects of dietary marjoram, Origanum majorana extract on growth performance, hematological, antioxidant, humoral and mucosal immune responses, and resistance of common carp, Cyprinus carpio against Aeromonas hydrophila Fish Shellfish Immun. Pub Date : 2020-11-28 Morteza Yousefi; Hamed Ghafarifarsani; Seyed Hossein Hoseinifar; Ghasem Rashidian; Hien Van Doan
The present study aimed to investigate the potential effects of dietary marjoram extract on growth performance, health, and disease resistance in common carp, Cyprinus carpio. To this purpose, the fish were assigned into four treatments and fed by diets supplemented with 0 (control), 100, 200, and 400 mg marjoram extract kg−1 over eight weeks and then challenged with Aeromonas hydrophila. According
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Alterations in the non-neuronal cholinergic system induced by in-vitro exposure to diazoxon in spleen mononuclear cells of Nile tilapia (O. niloticus) Fish Shellfish Immun. Pub Date : 2020-12-04 G.A. Toledo-Ibarra; M.I. Girón-Pérez; C.E. Covantes-Rosales; G.H. Ventura-Ramón; G. Pérez-Sánchez; A. López-Torres; K.J.G. Diaz-Resendiz; E. Becerril-Villanueva; L. Pavón
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Identification and characterization of a Toll-like receptor gene from Macrobrachium nipponense Fish Shellfish Immun. Pub Date : 2020-12-07 Hansam Cho; Ki Hoon Park; Yuyeon Jang; Yeondong Cho; Yoon-Ki Heo; Minjee Kim; Young Bong Kim
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Effect of a novel DNA vaccine against pancreas disease caused by salmonid alphavirus subtype 3 in Atlantic salmon (Salmo salar) Fish Shellfish Immun. Pub Date : 2020-12-04 Ragnar Thorarinsson; Jeffrey C. Wolf; Makoto Inami; Lisa Phillips; Ginny Jones; Alicia M. Macdonald; Jose F. Rodriguez; Hilde Sindre; Eystein Skjerve; Espen Rimstad; Øystein Evensen
Pancreas disease (PD) caused by salmonid alphavirus subtype 3 (SAV3) is a serious disease with large economic impact on farmed Norwegian Atlantic salmon production despite years of use of oil-adjuvanted vaccines against PD (OAVs). In this study, two commercially available PD vaccines, a DNA vaccine (DNAV) and an OAV, were compared in an experimental setting. At approximately 1040° days (dd) at 12 °C
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Gut immunity in European sea bass (Dicentrarchus labrax): a review Fish Shellfish Immun. Pub Date : 2020-12-04 S. Picchietti; A. Miccoli; A.M. Fausto
In this review, we summarize and discuss the trends and supporting findings in scientific literature on the gut mucosa immune role in European sea bass (Dicentrarchus labrax L.). Overall, the purpose is to provide an updated overview of the gastrointestinal tract functional regionalization and defence barriers. A description of the available information regarding immune cells found in two immunologically-relevant
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Immunogenicity study of OmpU subunit vaccine against Vibrio mimicus in yellow catfish, Pelteobagrus fulvidraco Fish Shellfish Immun. Pub Date : 2020-12-04 Yu Fu; Yong-An Zhang; Jinyu Shen; Jiagang Tu
The outer membrane protein U (OmpU) is a conserved outer membrane protein in a variety of pathogenic Vibrio species and has been considered as a vital protective antigen for vaccine development. Vibrio mimicus (V. mimicus) is the pathogen causing ascites disease in aquatic animals. In this study, the prokaryotically expressed and purified His-tagged OmpU of V. mimicus (His-OmpU) was used as a subunit
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Immunoglobulin T genes in Actinopterygii Fish Shellfish Immun. Pub Date : 2020-12-03 Serafin Mirete-Bachiller; David N. Olivieri; Francisco Gambón-Deza
In teleost fishes, there are three immunoglobulin isotypes named immunoglobulin M (IgM), D (IgD), and T (IgT). IgT was the last to be described in teleost fishes, and it is specific to them. From recent fish genomes, we identified and studied the immunoglobulin heavy chain genes in Actinopterygii. For this analysis, a custom bioinformatics and machine learning pipeline, we call CHfinder, was developed
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Antigenicity of hypothetical protein HP33 of Vibrio harveyi Y6 causing scale drop and muscle necrosis disease in Asian sea bass Fish Shellfish Immun. Pub Date : 2020-12-04 Anusara Kwankijudomkul; Ha Thanh Dong; Siwaporn Longyant; Paisarn Sithigorngul; Pongsak Khunrae; Triwit Rattanarojpong; Saengchan Senapin
A unique strain of Vibrio harveyi is the causative agent of scale drop and muscle necrosis disease (SDMND) in Asian sea bass (Lates calcarifer). This study investigated the protein profiles of SDMND-causing Vibrio harveyi isolates compared to the reference V. harveyi ATCC 14126 strain. A distinct protein band of 33 kDa, namely HP33, found from only V. harveyi SDMND was subjected to analysis by LC-MS/MS
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Comparative study on the effect of dietary β-carotene and phycocyanin extracted from Spirulina platensis on immune-oxidative stress biomarkers, genes expression and intestinal enzymes, serum biochemical in Nile tilapia, Oreochromis niloticus Fish Shellfish Immun. Pub Date : 2020-11-23 Mohamed S. Hassaan; Eman Y. Mohammady; Mohamed R. Soaudy; Soaad A. Sabae; Abeer M.A. Mahmoud; Ehab R. El-Haroun
The current trial investigated the roles of β-carotene and phycocyanin extracted from Spirulina platensis on growth, serum biochemical, digestive enzymes, antioxidant defense, immune responses, and immune gene expression in Nile tilapia (Oreochromis niloticus). Fish (1.52 ± 0.10 g) were randomly stocked to three treatments with three replicates (12 fish per replicate) in nine aquaria (60 L glass aquarium
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Lactobacillus plantarum in black soldier fly (Hermetica illucens) meal modulates gut health and immunity of freshwater crayfish (Cherax cainii) Fish Shellfish Immun. Pub Date : 2020-11-21 Md Javed Foysal; Ravi Fotedar; Muhammad A.B. Siddik; Md Reaz Chaklader; Alfred Tay
Probiotic supplements are being used to improve the growth and immune performance of aquaculture species over the last couple of decades. In recent times, black soldier fly (BSF) is considered as one of the promising sources of alternative protein to fishmeal protein in aqua-diets. Since the freshwater crayfish, marron (Cherax cainii), a Western Australian's native and iconic freshwater crayfish species
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Dietary Azomite, a natural trace mineral complex, improved the growth, immunity response, intestine health and resistance against bacterial infection in largemouth bass (Micropterus salmoides) Fish Shellfish Immun. Pub Date : 2020-11-25 Xiaoying Xu; Xiaoqin Li; Zhen Xu; Wenxiang Yao; Xiangjun Leng
Azomite is a hydrated calcium sodium aluminosilicat rich in rare earth elements. To investigate the dietary effects of Azomite on growth, intestine microbiota and morphology, immunohematological changes and disease resistance, seven diets with Azomite supplementation of 0 (the control), 1.0, 2.0, 3.0, 4.0, 5.0 and 6.0 g/kg (A0, A1, A2, A3, A4, A5, A6), were prepared and fed to largemouth bass, Micropterus
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The effect of ocean acidification on the enzyme activity of Apostichopus japonicus Fish Shellfish Immun. Pub Date : 2020-11-13 Wenge Shi; Yixuan Li; Yue Dong; Ming Xin; Xuelei Zhang; Qinzeng Xu
The influence of ocean acidification (OA) is particularly significant on calcifying organisms. The sea cucumber Apostichopus japonicus is an important cultured calcifying organism in the northern China seas. Little was known about the effects of OA on this economically important species. In this study, individuals from embryo to juveniles stage of A. japonicus, cultured in different levels of acidified
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Systemic and mucosal antibody response of freshwater cultured Asian seabass (Lates calcarifer) to monovalent and bivalent vaccines against Streptococcus agalactiae and Streptococcus iniae Fish Shellfish Immun. Pub Date : 2020-11-18 Nguyen Giang Thu Lan; Krishna R. Salin; Siwaporn Longyant; Saengchan Senapin; Ha Thanh Dong
Asian seabass, Lates calcarifer farming in Southeast Asia, encounters serious disease challenges caused by Streptococcus agalactiae and Streptococcus iniae. However, a vaccine for disease prevention is not yet available. In this study, we investigated the mucosal and systemic antibody (IgM) response kinetics of the Asian seabass following primary immunization with oil-based formalin-killed vaccines
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Transcription profile, NF-ĸB promoter activation, and antiviral activity of Amphiprion clarkii Akirin-2 Fish Shellfish Immun. Pub Date : 2020-11-28 K.A.S.N. Shanaka; K.P. Madushani; Rajamanthrilage Kasun Madusanka; M.D. Neranjan Tharuka; Sarithaa Sellaththurai; Hyerim Yang; Sumi Jung; Jehee Lee
Animal defense system constitutes a series of distinct mechanisms that specifically defend against microbial invasion. Understanding these complex biological mechanisms is of paramount importance for implementing disease prevention strategies. In this study, the transcription factor, Akirin-2 was identified from ornamental fish Amphiprion clarkii and its involvement in immune response was characterized
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Transcriptomic analysis reveals the immune changes associated with reproduction in the clam Meretrix petechialis Fish Shellfish Immun. Pub Date : 2020-11-27 Di Wang; Baozhong Liu
Substantial mortality and economic losses in marine mollusk culture has drawn considerable attention in recent years. The changes in immune status and environmental stress are thought to be the main causes of shellfish summer mortality. The reproduction and immune defense are both physiologically demanding processes, therefore, the immune status of mollusk is likely to be affected by reproduction during
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Characterization of a common carp intelectin gene with bacterial binding and agglutination activity Fish Shellfish Immun. Pub Date : 2020-11-26 Jinyi Li; Yanru Chen; Wei Gu; Fojiao Xu; Hua Li; Shijuan Shan; Xiaojie Sun; Miao Yin; Guiwen Yang; Lei Chen
Intelectin (ITLN) is a type of glycan-binding lectin involved in many physiological processes and some human diseases. Here we report a common carp intelectin (cITLN). Like other orthologs, cITLN also contains a conserved fibrinogen-related domain (FReD) and a unique intelectin domain, expresses in all the tissues tested with the highest level in the hindgut, and responds to bacterial challenge in
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In vitro and in vivo immunologic potentiation of herb extracts on shrimp (Litopenaeus vannamei) Fish Shellfish Immun. Pub Date : 2020-11-06 Yanjun Ding; Luqing Pan; Guorui Gao; Hui Huang
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A Litopenaeus vannamei TRIM32 gene is involved in oxidative stress response and innate immunity Fish Shellfish Immun. Pub Date : 2020-11-06 Lei Wang; Ke-Cheng Lu; Guo-Liang Chen; Ming Li; Chao-Zheng Zhang; Yi-Hong Chen
Tripartite motif (TRIM) family proteins are named by the presence of tripartite motifs in their amino terminal domains. Apart from the amino terminal, their carboxyl terminal contain variable domains which mediate diverse functions of the TRIM proteins. It had been found that TRIM proteins played important roles in distinct biological processes, such as innate immunity, anti-tumor immunity, cell cycle
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White shrimp Litopenaeus vannamei that have received mixtures of heat-killed and formalin-inactivated Vibrio alginolyticus and V. harveyi exhibit recall memory and show increased phagocytosis and resistance to Vibrio infection Fish Shellfish Immun. Pub Date : 2020-11-21 Chih-Hung Hsu; Jiann-Chu Chen; Yong-Chin Lin; Yu-Yuan Chen; Po-Chun Liu; Bo-Wei Lin; Jen-Fang Hsieh
Heat-killed Vibrio alginolyticus (HVa), formalin-inactivated V. alginolyticus (FVa), heat-killed Vibrio harveyi (HVh), formalin-inactivated V. harveyi (FVh), live V. alginolyticus (LVa), and live V. harveyi (LVh) were used in this study. White shrimp Litopenaeus vannamei receiving two mixtures (HVa + FVa) or four mixtures (HVa + FVa + HVh + FVh) served as primary exposure, and shrimp receiving LVa
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The alleviation of skin wound-induced intestinal barrier dysfunction via modulation of TLR signalling using arginine in gilthead seabream (Sparus aurata L) Fish Shellfish Immun. Pub Date : 2020-11-18 Zhichu Chen; Diana Ceballos-Francisco; Francisco A. Guardiola; Dong Huang; M. Ángeles Esteban
The present study sought to investigate the effect of arginine on the involvement of toll-like receptors (TLRs) in skin wound-induced intestinal barrier dysfunction in gilthead seabream (Sparus aurata L.). Two replicates of fish (n = 8) were fed a commercial diet (CON, total 2.75% arginine), CON diet enriched with 1% arginine (ARG1, total 3.65% arginine) and 2% arginine (ARG2, total 4.53% arginine)
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Effect of replacement of dietary fish oil with four vegetable oils on prostaglandin E2 synthetic pathway and expression of inflammatory genes in marine fish Larimichthys crocea Fish Shellfish Immun. Pub Date : 2020-11-02 Kun Cui; Xueshan Li; Qiang Chen; Qingfei Li; Shengnan Gao; Peng Tan; Kangsen Mai; Qinghui Ai
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BDE-47 exposure modulates cellular responses, oxidative stress and biotransformation related-genes in Mytilus galloprovincialis Fish Shellfish Immun. Pub Date : 2020-11-18 Concetta Maria Messina; Cristobal Espinosa Ruiz; Francesco Regoli; Simona Manuguerra; Fabio D'Agostino; Giuseppe Avellone; Mario Sprovieri; Andrea Santulli
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Pathogenicity and immunogenicity of Edwardsiella piscicida ferric uptake regulator (fur) mutations in zebrafish Fish Shellfish Immun. Pub Date : 2020-11-08 Banikalyan Swain; Cole T. Powell; Roy Curtiss
Edwardsiella piscicida is the etiological agent of edwardsiellosis in fish and causes severe economic losses in global aquaculture. Vaccination would be the most effective method to prevent infectious diseases and their associated economic losses. The ferric uptake regulator (Fur) is an important transcriptional global regulator of Gram-negative bacteria. In this study, we examined the regulatory function
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Functional characterization and gene expression profile of perforin-2 in starry flounder (Platichthys stellatus) Fish Shellfish Immun. Pub Date : 2020-11-17 Kwang-Min Choi; Dong-Hee Cho; Min-Soo Joo; Hye-Sung Choi; Myoung Sug Kim; Hyun-Ja Han; Mi Young Cho; Seong Don Hwang; Do-Hyung Kim; Chan-Il Park
The membrane attack complex/perforin (MACPF) superfamily consists of multifunctional proteins that form pores on the membrane surface of microorganisms to induce their death and have various immune-related functions. PFN2 is a perforin-like protein with an MACPF domain, and humans with deficient PFN2 levels have increased susceptibility to bacterial infection, which can lead to fatal consequences for
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