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Molecular characterization, antibacterial and immunoregulatory activities of liver-expressed antimicrobial peptide 2 in black rockfish, Sebastes schlegelii Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-28 Min Zhang, Xue Yan, Chang-biao Wang, Wen-qing Liu, Yue Wang, Hao Jing, Bing Wang, Kai Yang, Zi-yue Chen, Yu-yu Luan, Guang-hua Wang
LEAP2 (liver expression antimicrobial peptide 2), is an antimicrobial peptide widely found in vertebrates and mainly expressed in liver. LEAP2 plays a vital role in host innate immunity. In teleosts, a number of LEAP2 homologs have been reported, but their effects on host defense are still limited. In this study, a LEAP2 homolog () was identified from black rockfish, , and its structure, expression
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Extracellular ATP- and adenosine-mediated purinergic signaling modulates inducible nitric oxide synthase (iNOS) gene expression, enzyme activity and nitric oxide production in common carp (Cyprinus carpio) head kidney macrophages Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-27 Shuo Li, Xiwen Cui, Yue Cao, Jinsheng Sun
Inducible nitric oxide (NO) synthase (iNOS) is a key immune mediator for production of inflammatory mediator NO from -arginine. Tight regulation of iNOS expression and enzyme activity is critical for proper NO productions under inflammation and infection conditions. However, the regulatory mechanism for iNOS expression and enzyme activity in fish remains largely unknown. Here, we show that extracellular
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Biocompatibility of intraperitoneally implanted TEMPO-oxidized cellulose nanofiber hydrogels for antigen delivery in Atlantic salmon (Salmo salar L.) vaccines Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-25 Sarah M. Turner, Kora Kukk, Inga F. Sidor, Michael D. Mason, Deborah A. Bouchard
Disease outbreaks are a major impediment to aquaculture production, and vaccines are integral for disease management. Vaccines can be expensive, vary in effectiveness, and come with adjuvant-induced adverse effects, causing fish welfare issues and negative economic impacts. Three-dimensional biopolymer hydrogels are an appealing new technology for vaccine delivery in aquaculture, with the potential
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Bioinformatics characteristics and expression analysis of IL-8 and IL-10 in largemouth bass (Micropterus salmoides) upon Nocardia seriolae infection Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-25 Qin Wang, Zhang chun Zhao, Ruhan Sun, Zechao Shi, Yaqian Zhang, Bingchao Wang, Xuezhen Zhang, Wei Ji
IL-8 and IL-10 are crucial inflammatory cytokines that participate in defending host cells against infections. To demonstrate the function of the two interleukin genes in largemouth bass (), we initially cloned and identified the cDNA sequences of and in largemouth bass, referred to as and , respectively. The open reading frame (ORF) of was 324 bp in length, encoding 107 amino acids, while the ORF
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Dietary methanotroph bacteria meal alleviates soybean meal-induced enteritis by improving immune tolerance and intestinal flora profile of juvenile turbot (Scophthalmus maximus L.) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-23 Jichang Zheng, Wencong Zhang, Zhijie Dan, Xiufei Cao, Ye Gong, Kangsen Mai, Qinghui Ai
An 8-week growth trial was performed to investigate the protective effects of methanotroph bacteria meal (MBM) produced from methane against soybean meal-induced enteritis (SBMIE) in juvenile turbot ( L.). Five isonitrogenous and isolipidic diets were formulated: fishmeal-based diet (FM, the control group); FM with approximate 50% of fishmeal substituted by 399.4 g/kg soybean meal (SBM); SBM supplemented
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Functional identification of two HMGB1 paralogues provides insights into autophagic machinery in teleost Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-21 Dan Wang, Xiaoyu Ma, Hengyi Hu, Jingqi Ren, Jiaxi Liu, Hong Zhou
High mobility group box 1 (HMGB1) is a multifunctional regulator that plays different roles in various physiological and pathological processes including cell development, autophagy, inflammation, tumor metastasis, and cell death based on its cellular localization. Unlike mammalian HMGB1, two HMGB1 paralogues (HMGB1a and HMGB1b) have been found in fathead minnow and other fish species and its function
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Effects of different concentrations and particle sizes of nanoplastics on gut microbiology, metabolism, and immunity in Chiromantes dehaani Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-20 Mingming Han, Tian Zhu, Zihan Zhou, Qin Si, Chenxi Zhu, Yiming Li, Qichen Jiang
This study investigated the effects of nanoplastics (NPs) of varying particle sizes (75, 500, and 1000 nm) and concentrations (2.5 and 10 mg/L) on the gut health of . The experimental groups included a control (Cg0), and varying combinations of particle size and concentration. Our results showed that 75 nm NPs were more likely to enhance pathogenic bacterial growth than other sized NPs. Compared with
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Damage of polyethylene microplastics on the intestine multilayer barrier, blood cell immune function and the repair effect of Leuconostoc mesenteroides DH in the large-scale loach (Paramisgurnus dabryanus) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-19 Xiaohua Xia, Xiaoyu Ma, Ning Liang, Lu Qin, Weiran Huo, Yi Li
Polyethylene microplastics (PE-MPs) has become a global concern due to their widespread distribution and hazardous properties in aquatic habitats. In this study, the accumulation effect of PE-MPs in the intestine of large-scale loach () was explored by adding different concentrations of PE-MPs to the water, the destination of PE-MPs after breaking the intestinal barrier and the effects caused. The
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Transcriptomic analysis of turbot (Scophthalmus maximus) treated with zymosan a reveals that lncRNAs and inflammation-related genes mediate the protection conferred against Aeromonas salmonicida Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-17 Alejandro Romero, Magalí Rey-Campos, Patricia Pereiro, Marta Librán-Pérez, Antonio Figueras, Beatriz Novoa
is one of the most harmful pathogens in finfish aquaculture worldwide. Immunostimulants such as β-glucans are used to enhance the immunity of cultured fish. However, their effects on fish physiology are not completely understood. In the present work, we evaluated the effect of a single intraperitoneal (ip) injection of zymosan A on fish survival against infection. A single administration of this compound
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A comprehensive review on the utilization of probiotics in aquaculture towards sustainable shrimp farming Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-17 C.M. Mathan Muthu, A.S. Vickram, B. Bhavani Sowndharya, A. Saravanan, R. Kamalesh, Yuvaraj Dinakarkumar
Probiotics in shrimp aquaculture have gained considerable attention as a potential solution to enhance production efficiency, disease management, and overall sustainability. Probiotics, beneficial microorganisms, have shown promising effects when administered to shrimp as dietary supplements or water additives. Their inclusion has been linked to improved gut health, nutrient absorption, and disease
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Mechanism of Chinese sturgeon IFN-γ inhibition on Mycobacterium marinum (Acipenser sinensis) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-17 Ting Yu, Meng Xie, Kai Luo, Xiao Zhang, Weihua Gao, Qiaoqing Xu, Shuhuan Zhang
IFN-γ plays a crucial role in both innate and adaptive immune responses and is a typical Th1 cytokine that promotes Th1 response and activates macrophages. When macrophages were incubated with IFN-γ, their phagocytosis ratio against increased significantly, as observed under fluorescence microscopy. The macrophages engulfed a large number of . The proliferative ability of macrophages treated with IFN-γ
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The interaction between lipid and vitamin D3 impacts lipid metabolism and innate immunity in Chinese mitten crabs Eriocheir sinensis Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-16 Shubin Liu, Qiuran Yu, Jiadai Liu, Han Wang, Xiaodan Wang, Chuanjie Qin, Erchao Li, Jianguang Qin, Liqiao Chen
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The physiological activities of fucoidan and its application in animal breeding Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-16 Yusi Lan, Keqiang Qin, Shengjun Wu
Fucoidan, a water-soluble heteropolysaccharide predominantly found in brown algae, comprises active components such as fucose and sulfate groups. This polysaccharide exhibits a range of physiological activities, including antioxidant, antiviral, anticancer, and immunomodulatory activities. In light of the global prohibition of antibiotics in animal feed, there is increasing interest in identifying
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miR-193b-5p promotes GCRV replication by inhibiting autophagy via targeting deptor in grass carp (Ctenopharyngodon idellus) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-15 Hongyan Yu, Zheyan Chen, Qiaozhen Yu, Yubang Shen, Lang Gui, Xiaoyan Xu, Jiale Li
miRNAs are increasingly recognized for their crucial role in autophagy processes. Recent research has highlighted the significant function of autophagy in modulating immune responses. Within this context, specific miRNAs have been identified as indirect mediators of immune functions through their modulation of autophagy. In this study, we verified that miR-193b-5p simultaneously targeted the grass
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Effects of antimicrobial peptides from dietary Hermetia illucens larvae on the growth, immunity, gene expression, intestinal microbiota and resistance to Aeromonas hydrophila of juvenile red claw crayfish (Cherax quadricarinatus) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-14 Ze-Long Zhang, Yong-Qi Meng, Jia-Jun Li, Xiu-Xia Zhang, Jun-Tao Li, Jia-Rui Xu, Pei-Hua Zheng, Jian-An Xian, Yao-Peng Lu
Antimicrobial peptides (AMPs), which are widely present in animals and plants, have a broad distribution, strong broad-spectrum antibacterial activity, low likelihood of developing drug resistance, high thermal stability and antiviral properties. The present study investigated the effects of adding AMPs from larvae on the growth performance, muscle composition, antioxidant capacity, immune response
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Efficacy of nutritional selenium nanoparticles on growth performance, immune response, antioxidant capacity, expression of growth and immune-related genes, and post-stress recovery in juvenile Sobaity seabream (Sparidentex hasta) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-13 Seyed Eisa Abdollahi-Mousavi, Saeed Keyvanshokooh, Mansour Torfi Mozanzadeh, Ahmad Ghasemi
This study evaluated the impacts of nano-Se on the growth, immunity, antioxidant capacity, physiological parameters, gene expression, and stress resistance of fingerling Sobaity seabream (). The fish with an average weight of 21.5 ± 0.1 g were divided into four treatment groups in triplicates that received one of the test diets supplemented with varying levels of nano-Se: 0 (control), 0.5 (Se-0.5)
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Zebrafish ANGPT4, member of fibrinogen-related proteins, is an LTA-, LPS- and PGN-binding protein with a bacteriolytic activity Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-13 Yang Zhou, Lu Chen, Songtao Hao, Xianke Cao, Shousheng Ni
Fibrinogen-related proteins (FREPs) are a family of glycoproteins that contain a fibrinogen-like (FBG) domain. Many members of FREPs have been shown to play an important role in innate immune response in both vertebrates and invertebrates. Here we reported the immune functional characterization of ANGPT4, member of FREPs, in zebrafish . Quantitative real time PCR showed that the expression of zebrafish
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Asymmetric evolution of ISG15 homologs and the immune adaptation to LBUSV infection in spotted seabass (Lateolabrax maculatus) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-12 Bo Zhang, Lulu Yan, Changhong Lin, Yong Liu, Chao Zhao, Pengfei Wang, Bo Zhang, Yanhong Zhang, Lihua Qiu
The battle between host and viral is ubiquitous across all ecosystems. Despite this, research is scarce on the antiviral characteristics of fish, particularly in those that primarily rely on innate immune responses. This study, comprehensively explored the genetic and antiviral features of ISG15 in spotted seabass, focusing on its response to largemouth bass ulcerative syndrome virus (LBUSV). Through
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Functional analyses of TRAF6 gene in Argopecten scallops Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-12 Xiaotong Zhang, Xia Wang, Jieyu Chen, Min Chen, Xia Lu, Junhao Ning, Haijun Liu, Guilong Liu, Xin Xu, Xiaoxu Qu, Kai Yu, He Xu, Chunde Wang, Bo Liu
The tumor necrosis factor (TNF) receptor-associated factor (TRAF) family has been reported to be involved in many immune pathways. In a previous study, we identified 5 TRAF genes, including TRAF2, 3, 4, 6, and 7, in the bay scallop (, Air) and the Peruvian scallop (, Apu). Since TRAF6 is a key molecular link in the TNF superfamily, we conducted a series of studies targeting the gene in the Air and
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Unraveling the Red Sea soft coral Sarcophyton convolutum potentials against oxidative and inflammatory stresses in zebrafish Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-12 Khaled Mohammed-Geba, Reham Salah ElShaarawy, AbdAllah Alian, Hany Mohammed Ibrahim, Asmaa Galal-Khallaf
The Red Sea is one of the world's hotspots for biodiversity, and for marine natural products (MNPs) as well. These MNPs attract special interest for their capabilities to combat inflammatory and oxidative stress-related diseases, being some of the most serious health problems worldwide nowadays. The current study aimed to identify the bioactive ingredients of the Red Sea soft coral , and to assess
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Unravelling the main immune repertoire of Paracentrotus lividus following Vibrio anguillarum bath challenge Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-10 Sergio Fernández-Boo, André Machado, L. Filipe C. Castro, Rita Azeredo, Benjamin Costas
is the most abundant echinoid species in the North East Atlantic Ocean and Mediterranean Sea. Although there is abundant genomic information of the species, there is no deep characterisation of the genes involved in the immune response. Here, a reference transcriptome of male and female coelomocytes was produced. The generated transcriptome assembly has 203,511 transcripts, N50 transcript length of
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Molecular and functional characterization of a type-1 cystatin in amphioxus (Branchiostoma japonicum) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-09 Ronghuan Du, Xian Zheng, Yudan Liu, Pei Lu, Yuxiang Hong, Peng Wang
Cystatins comprise a vast superfamily of evolutionary conserved proteins, predominantly recognized for their roles as endogenous inhibitors by regulating the activity of cysteine proteases. Emerging lines of research evidence also provides insight into their alternative roles in a spectrum of biological and pathological processes, including neurodegenerative disorders, tumor progression, inflammatory
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rpoS involved in immune response of Macrobrachium nipponens to Vibrio mimicus infection Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-09 Ziyan Jiang, Lijie Qin, Anting Chen, Xinzhe Tang, Weifeng Gao, Xiaojian Gao, Qun Jiang, Xiaojun Zhang
is a pathogenic bacterium that cause red body disease in , leading to high mortality and financial loss. Based on previous studies, gene contribute to bacterial pathogenicity during infection, but the role of RpoS involved in the immune response of under infection remains unclear. In this study, the pathogen load and the RNA-seq of under wild-type and Δ strain infection were investigated. Over the
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Immune response and apoptosis of gibel carp (Carassius auratus gibelio) gills to Chilodonella hexasticha infection: Insights from histopathological and multi-omics analyses Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-09 Xialian Bu, Weishan Zhao, Hong Zou, Wenxiang Li, Ming Li, Guitang Wang
Gibel carp () is an important economically farmed fish in China. parasitizes on the gills and fins of host fish, causing disruption to their normal respiration and movement, ultimately resulting in death of the fish. In this study, a combination of histopathological, immunohistochemical, transferase dUTP nick end labeling (TUNEL), multi-omics, and molecular approaches were employed to identify the
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Oral immunization with recombinant L. lactis expressing GCRV-II VP4 produces protection against grass carp reovirus infection Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-08 Wu Huiliang, Jiyuan Yin, Li Siming, Wang Hao, Jiang Peng, L.I. Pengfei, Ding Zhaoyang, Yan Han, Chen Bo, Wang Linchuan, Wang Qing
The hemorrhagic disease causing by grass carp reovirus (GCRV) infection, is associated with major economic losses and significant impact on aquaculture worldwide. VP4 of GCRV is one of the major outer capsid proteins which can induce an immune response in the host. In this study, pNZ8148 was constructed to express recombinant VP4 protein of GCRV, which was confirmed by the Western-Blot and enzyme-linked
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Coxsackievirus and adenovirus receptor inhibits tilapia lake virus infection via binding to viral segment 8 and 10 encoded protein Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-08 Jing Wen, Zishan Ke, Yingying Wang, Yingying Li, Defeng Zhang, Xubing Mo, Jiyuan Yin, Cunbin Shi, Wenli Zhou, Shucheng Zheng, Qing Wang
The global aquaculture industry of tilapia () has been significantly impacted by the emergence of tilapia lake virus (TiLV). However, effective prevention and control measures are still not available due to a lack of unclear pathogenesis of TiLV. Our previous transcriptome found that coxsackievirus and adenovirus receptor (CAR) was in response to TiLV infection in tilapia. To explore the potential
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Structure and function analyses of the SRC gene in Pacific white shrimp Litopenaeus vannamei Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-07 Shuqing Si, Xiaojun Zhang, Yang Yu, Xiaoyun Zhong, Xiaoxi Zhang, Jianbo Yuan, Fuhua Li
SRC gene encodes scavenger receptor class C, a member of the scavenger receptor family, and has only been identified and investigated in invertebrates. Our previous studies have revealed that is a novel candidate gene associated with body weight in Pacific white shrimp (). In order to comprehend the underlying mechanism by which affects shrimp growth, we analyzed the structure, phylogeny, expression
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Characterization of a pseudohemocyanin gene (PtPhc1) and its immunity function in response to Vibrio parahaemolyticus infection in the swimming crab Portunus trituberculatus Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-07 Yukun Li, Jianjian Lv, Dongfang Sun, Junyang Guo, Ping Liu, Baoquan Gao
Pseudohemocyanin is a member of the hemocyanin superfamily, but little research is available on its function in immunology. In this study, a pseudohemocyanin gene, named , was obtained by gene cloning. The cDNA was 2312 bp in length, encoding 684 amino acids while exhibiting a characteristic hemocyanin structural domain. Tissue expression analysis revealed ubiquitous expression of across all tissues
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Involvement of tumor necrosis factor receptor-associated factor 6 (TRAF6) in NF-κB activation and antiviral immunity: Molecular and functional characterization of TRAF6 in red-spotted grouper (Epinephelus akaara) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-07 E.M.T. Tharanga, Kishanthini Nadarajapillai, D.M.K.P. Sirisena, Gaeun Kim, Taehyug Jeong, Qiang Wan, Jehee Lee
Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a member of the TRAF family of adaptor proteins involved in the signal transduction pathways of both TNF receptor and interleukin-1 receptor/Toll-like receptor superfamilies. In this study, red-spotted grouper () TRAF6 (EaTraf6) was identified and characterized. The open reading frame of 1713 bp in length, encodes a putative protein of 570
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Persistent immune responses in the heart determine the outcome of cardiomyopathy syndrome in Atlantic salmon (Salmo salar) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-06 Baojian Sun, Marco A. Vindas, Simona Kavaliauskiene, Håvard Bjørgen, Erling Olaf Koppang, Helene Wisløff, Michael Frisk, Hege Lund, Ida B. Johansen
Cardiomyopathy syndrome (CMS) caused by piscine myocarditis virus (PMCV) is a severe cardiac disease in Atlantic salmon () and one of the leading causes of morbidity and mortality in the Norwegian aquaculture industry. Previous research suggest a variation in individual susceptibility to develop severe disease, however the role of the immune response in determining individual outcome of CMS is poorly
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MicroRNA sequencing analysis reveals immune responses in hepatopancreas of Fenneropenaeus penicillatus under white spot syndrome virus infection Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-06 Shengping Zhong, Xiaowu Ye, Hongtao Liu, Xiaowan Ma, Xiuli Chen, Longyan Zhao, Guoqiang Huang, Lianghua Huang, Yongzhen Zhao, Ying Qiao
White Spot Disease is one of the most harmful diseases of the red tail shrimp, which can cause devastating economic losses due to the highest mortality up to 100% within a few days. MicroRNAs (miRNAs) are large class of small noncoding RNAs with the ability to post-transcriptionally repress the translation of target mRNAs. MiRNAs are considered to have a significant role in the innate immune response
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Integrated characterizations of intestinal bacteria and transcriptomics revealed the acute stress response to carbonate alkalinity in white shrimp Penaeus vannamei Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-06 Zule Song, Kui Li, Kejun Li
The impact of carbonate alkalinity in saline-alkaline water on aquatic organisms, particularly , a significant species in aquaculture, remains a critical area of study. To elucidate the acute response mechanisms of to elevated carbonate alkalinity environments, we utilized 16S rRNA gene and transcriptome sequencing technologies to analyze intestinal bacteria and gene expressions within various tissues
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OmpU and OmpC are the key OMPs for Litopenaeus vannamei hemocyanin recognizes Vibrio parahaemolyticus Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-06 Xianliang Zhao, Yao Liu, Fang Yan, Zhongyang Lin, Yongzhen Zhao, Xiuli Chen, Yueling Zhang
Hemocyanin is a multifunctional protein present in arthropods and mollusks, responsible for oxygen transport and participating in multiple roles of immune defense including antibacterial activity. However, the molecular basis of how hemocyanin recognizes pathogens and exerts antibacterial activity remains poorly understood. In the present study, the pull-down assay was used to isolate outer membrane
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Single-cell transcriptome landscape of zebrafish liver reveals hepatocytes and immune cell interactions in understanding nonalcoholic fatty liver disease Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-05 Yingyi Huang, Xiang Liu, Hong-Yan Wang, Jian-Yang Chen, Xianghui Zhang, Yubang Li, Yifang Lu, Zhongdian Dong, Kaiqiang Liu, Zhongduo Wang, Qian Wang, Guangyi Fan, Jun Zou, Shanshan Liu, Changwei Shao
Nonalcoholic fatty liver disease (NAFLD) is becoming the most common chronic liver disease in the world. Immunity is the major contributing factor in NAFLD; however, the interaction of immune cells and hepatocytes in disease progression has not been fully elucidated. As a popular species for studying NAFLD, zebrafish, whose liver is a complex immune system mediated by immune cells and non-immune cells
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Subgenomic T cell receptor alpha and delta (TRA/TRD) loci in common carp Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-05 Masaharu Okano, Jiro Miyamae, Kohei Sakurai, Takuya Yamaguchi, Ren Uehara, Fumihiko Katakura, Tadaaki Moritomo
In jawed vertebrates, the T cell receptor alpha (TRA) and delta (TRD) genes, which encode the TRα and TRδ chains, respectively, are located as a nested structure on a single chromosome. To date, no animal has been reported to harbor multiple TRA/TRD loci on different chromosomes. Therefore, herein, we describe the first full annotation of the TRA/TRD genomic regions of common carp, an allo-tetraploid
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Excessive dietary iron exposure increases the susceptibility of largemouth bass (Micropterus salmoides) to Aeromonas hydrophila by interfering with immune response, oxidative stress, and intestinal homeostasis Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-05 Xiaoli Chen, Hong Liu, Shuangping Liu, Zhifeng Zhang, Xiong Li, Jian Mao
Iron is an essential cofactor in the fundamental metabolic pathways of organisms. Moderate iron intake can enhance animal growth performance, while iron overload increases the risk of pathogen infection. Although the impact of iron on the pathogen-host relationship has been confirmed in higher vertebrates, research in fish is extremely limited. The effects and mechanisms of different levels of iron
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Identification of antibacterial activity of liver-expressed antimicrobial peptide 2 (LEAP2) from primitive vertebrate lamprey Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-03 Yaocen Wang, Zhuoying Wang, Zhanfeng Gao, Yimu Luan, Qingwei Li, Yue Pang, Meng Gou
Liver-expressed antimicrobial peptide 2 (LEAP2) is a member of the antimicrobial peptides family and plays a key role in the innate immune system of organisms. LEAP2 orthologs have been identified from a variety of fish species, however, its function in primitive vertebrates has not been clarified. In this study, we cloned and identified Lc-LEAP2 from the primitive jawless vertebrate lamprey () which
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Evaluation of immunogenicity and protective efficacy of the outer membrane porin F (OprF) against Pseudomonas plecoglossicida in large yellow croaker (Larimichthys crocea) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-03 Liangyin He, Jiale Kang, Xingfu Chen, Pan Qin, Xinhua Chen
Large yellow croaker () farming dominates the marine aquaculture industry in China. However, the epidemic outbreaks of visceral white nodules disease (VWND), caused by bacterial pathogen , have emerged as a significant concern within the large yellow croaker industry. Although vaccination is considered to be an effective method for preventing and controlling infection, there is currently no commercially
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GSTP1 is a negative regulator of MAVS in the antiviral signaling against SVCV infection Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-03 Shuaibin Pei, Yingbing Wei, Zhenghao Li, Huijuan Zhong, Jinyang Dong, Zewen Yi, Ruixin Hou, Weiguang Kong, Jun Xiao, Zhen Xu, Hao Feng
Glutathione -transferase P1 (GSTP1), the most ubiquitous member of the GST superfamily, plays vital roles in the detoxification, antioxidant defense, and modulation of inflammatory responses. However, limited studies have been conducted on the function of GSTP1 in antiviral innate immunity. In this study, we have cloned the homolog of GSTP1 in triploid hybrid crucian carp (3nGSTP1) and investigated
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Genetically engineering of Saccharomyces cerevisiae for enhanced oral delivery vaccine vehicle Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-03 Baoquan Han, Feng Yue, Xiaojun Zhang, Kun Xu, Zhiying Zhang, Zhongyi Sun, Lu Mu, Xiaoyu Li
As a series of our previous studies reported, recombinant yeast can be the oral vaccines to deliver designed protein and DNA, as well as functional shRNA, into dendritic cells (DCs) in mice for specific immune regulation. Here, we report the further optimization of oral yeast-based vaccine from two aspects (yeast characteristics and recombinant DNA constitution) to improve the effect of immune regulation
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Acrylamide exposure induces growth retardation, neurotoxicity, stress, and immune/antioxidant disruption in Nile tilapia (Oreochromis niloticus): The alleviative effects of Chlorella vulgaris diets Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-01-30 Asmaa Edrees, Nema S. Shaban, Nour El-Houda Y. Hassan, Asmaa S.A. Abdel-Daim, Mohamed S. Sobh, Rowida E. Ibrahim
This study looked at the toxic impacts of water-born acrylamide (ACR) on Nile tilapia (Oreochromis niloticus) in terms of behaviors, growth, immune/antioxidant parameters and their regulating genes, biochemical indices, tissue architecture, and resistance to Aeromonas hydrophila. As well as the probable ameliorative effect of Chlorella vulgaris (CV) microalgae as a feed additive against ACR exposure
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Identifying the in vivo-induced antigenic genes is a strategy to develop DNA vaccine against Nocardia seriolae in hybrid snakehead (Channa maculata ♀ × Channa argus ♂) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-02 Tingting Weng, Guoquan Chen, Na Li, Wanna Sirimanapong, Ting Huang, Jianlin Chen, Liqun Xia
has been identified as the causative agent of fish nocardiosis, resulting in serious economic losses in aquaculture. With an aim to screen potential candidates for vaccine development against , the -induced genes of . in hybrid snakehead ( ♂) model were profiled via -induced antigen technology (IVIAT) in the present study, and 6 -induced genes were identified as follows: IS701 family transposase ()
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A comprehensive transcriptional body map of Atlantic salmon unveils the vital role of the intestine in the immune system and highlights functional specialization within its compartments Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-02 Trond M. Kortner, Sergey Afanasyev, Erling Olaf Koppang, Håvard Bjørgen, Åshild Krogdahl, Aleksei Krasnov
The intestine is a barrier organ that plays an important role in the immune system of Atlantic salmon. The immune functions are distributed among the diffuse gut lymphoid tissue containing diverse immune cells, and other cell types. Comparison of intestinal transcriptomes with those of other organs and tissues offers an opportunity to elucidate the specific roles of the intestine and its relationship
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Characterization and functional analysis of SOCS9 from orange-spotted grouper (Epinephelus coioides) during virus infection Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-02 Junxi Chen, Zhaofeng Chen, Yi Li, Bei Wang, Yishan Lu, Jichang Jian, Jufen Tang, Jia Cai
The suppressor of cytokine signaling (SOCS) proteins family have twelve members including eight known mammalian SOCS members (CISH, SOCS1-7) and four new discovery members (SOCS3b, SOCS5b, SOCS8 and SOCS9) that is regarded as a classic feedback inhibitor of cytokine signaling. Although the function of the mammalian SOCS proteins have been well studied, little is known about the roles of SOCS in fish
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Regulation of NF-κB signaling by NLRC (NLRC3-like) gene in the common carp (Cyprinus carpio) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-01-30 Cuixia Wang, Jiahui Xu, Yingying Zhang, Dongchun Yan, Lingjun Si, Linrui Chang, Ting Li
Among teleost NLRs, NLR-C subfamily is a large group of proteins that were teleost-specific and evolution analysis showed that NLR-Cs are most likely to evolve from NLRC3 gene (thus also called as NLRC3Ls). Presently, although there have been rich studies investigating teleost NLRC3 and NLRC3L, the data on the regulatory mechanism was limited. In this study, immune regulation of inflammatory signaling
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Edwardsiella piscicida causes iron storage disorders by an autophagy pathway in fish monocytes/macrophages Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-01-30 Jingqi Ren, Xiaoyu Ma, Hengyi Hu, Dan Wang, Hao Sun, Jiaxi Liu, Xinyan Wang, Hong Zhou
Edwardsiella piscicida (E. piscicida) is a gram-negative pathogen that survives in intracellular environment. Currently, the interplay between E. piscicida and host cells has not been completely explored. In this study, we found that E. piscicida disturbed iron homeostasis in grass carp monocytes/macrophages to maintain its own growth. Further investigation revealed the bacteria induced an increase
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Novel protective aspects of dietary polyphenols against pesticidal toxicity and its prospective application in rice-fish mode: A Review Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-01 Rabia Tahir, Fozia Afzal, Ji Liang, Song Yang
The rice fish system represents an innovative and sustainable approach to integrated farming, combining rice cultivation with fish rearing in the same ecosystem. However, one of the major challenges in this system is the pesticidal pollution resulting from various sources, which poses risks to fish health and overall ecosystem balance. In recent years, dietary polyphenols have emerged as promising
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Sea perch (Lateolabrax japonicus) UBC9 augments RGNNV infection by hindering RLRs-interferon response Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-01 Xiaoqi Chen, Wanwan Zhang, Hao Huang, Meisheng Yi, Kuntong Jia
Small ubiquitin-like modifier (SUMO) is a reversible post-translational modification that regulates various biological processes in eukaryotes. Ubiquitin-conjugating enzyme 9 (UBC9) is the sole E2-conjugating enzyme responsible for SUMOylation and plays an important role in essential cellular functions. Here, we cloned the UBC9 gene from sea perch () (LjUBC9) and investigated its role in regulating
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Selenium yeast improve growth, serum biochemical indices, metabolic ability, antioxidant capacity and immunity in black carp Mylopharyngodn piceus Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-01 Penghui Zhang, Chen Zhang, Xinfeng Yao, Yuanyuan Xie, Hao Zhang, Xianping Shao, Xia Yang, Qin Nie, Jinyun Ye, Chenglong Wu, Haifeng Mi
This experiment was conducted to investigate the impacts of dietary selenium yeast (SeY) on the growth performance, fish body composition, metabolic ability, antioxidant capability, immunity and inflammatory responses in juvenile black carp (). The base diet was supplemented with 0.00, 0.30 and 0.60 g/kg SeY (0.04, 0.59 and 1.15 mg/kg of selenium) to form three isonitrogenous and isoenergetic diets
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Assessing the role of ulvan as immunonutrient in Solea senegalensis Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-02-01 Marian Ponce, Victoria Anguís, Catalina Fernández-Díaz
Immunonutrition is a promising and viable strategy for the development of prophylactic measures in aquaculture. Ulvan, a sulphated marine polysaccharide from green seaweeds, has many biological activities including the immunomodulatory ones. The aim of this study was to assess the short and long-term effects of an ulvan-rich extract obtained from as immunonutrient in Senegalese sole juveniles. In this
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Tetraspanin CD53 regulates peripheral blood leucocytes vitality and pathogen infection in turbot (Scophthalmus maximus) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-01-29 Jian Zhang, Dandan Zhou, Binzhe Zhang, Xuepeng Li, Xudong Jiao
Cluster of differentiation 53 (CD53) also known as OX44 or tetraspanin 25 (TSPAN25) is a glycoprotein belonging to the tetraspanin family. Members of the tetraspanin family are characterized by four transmembrane domains, including intracellular N- and C-termini, and small and large extracellular domains. Currently, the function of CD53 in teleost is not well understood. In this study, we identified
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Characterization of type II IFNs and their receptors in a cyprinid fish, the blunt snout bream Megalobrama amblycephala Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-01-26 Bo Li, Shan Nan Chen, Lin Huang, Li Li, Li Ren, Jing Hou, Jing Yun Tian, Lan Hao Liu, P. Nie
Type II interferons (IFNs) are a key class of molecules regulating innate and adaptive immunity in vertebrates. In the present study, two members of the type II IFNs, IFN-γ and IFNγ-rel, were identified in the blunt snout bream (Megalobrama amblycephala). The open reading frame (ORF) of IFN-γ and IFNγ-rel was found to have 564 bp and 492 bp, encoding 187 and 163 amino acids, with the first 26 and 24
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Grass carp peroxiredoxin 5 and 6-mediated autophagy inhibit grass carp reovirus replication and mitigate oxidative stress Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-01-30 Qian Wang, Xinyu Liang, Hanyue Wang, Cheng Yang, Yongming Li, Lanjie Liao, Zuoyan Zhu, Yaping Wang, Libo He
Peroxiredoxins (Prxs) are a family of antioxidant enzymes crucial for shielding cells against oxidative damage from reactive oxygen species (ROS). In this study, we cloned and analyzed two grass carp peroxiredoxin genes, CiPrx5 and CiPrx6. These genes exhibited ubiquitous expression across all sampled tissues, with their expression levels significantly modulated upon exposure to grass carp reovirus
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Innate lymphoid cells (ILCs) in teleosts against data on ILCs in humans Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-01-29 Michał Stosik, Beata Tokarz-Deptuła, Wiesław Deptuła
It is assumed that cells corresponding to innate lymphoid cells (ILCs) in humans, in addition to lymphoid tissue inducer cells (LTi), are also found in teleosts. In this systematic group of organisms, however, they are a poorly understood cell population. In contrast to the data on ILCs in humans, which also remain incomplete despite advanced research, in teleosts, these cells require much more attention
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Antibacterial activity and modes of action of a novel hepcidin isoform from the shrimp scad, Alepes djedaba (Forsskål, 1775) Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-01-24 V.V. Anooja, K. Archana, P.P. Athira, M.V. Anju, S. Neelima, S. Muhammed Musthafa, M. Dhaneesha, T.P. Sajeevan, I.S. Bright Singh, Rosamma Philip
Hepcidin, initially identified in human blood ultrafiltrate as cysteine rich Liver Expressed Antimicrobial Peptide (LEAP-1), is a core molecular conduit between iron trafficking and immune response. Though a great share of studies has been focused on the iron regulatory function of hepcidins, investigations on the antimicrobial aspects are relatively less. The present study is aimed at identification
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Chlorogenic acid improves common carp (Cyprinus carpio) liver and intestinal health through Keap-1/Nrf2 and NF-κB signaling pathways: Growth performance, immune response and antioxidant capacity Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-01-23 Guo-Jun Shang, Si-ying Liu, Rui Zhu, Deng-Lai Li, Si-Tong Meng, Yin-tao Wang, Li-Fang Wu
In this experiment, we investigated the effects of adding chlorogenic acid (CGA) to the diet on growth performance, immune function, inflammation response, antioxidant capacity and its related mechanisms of common carp (Cyprinus carpio). A total of 600 fish were selected and randomly divided into five treatment groups and fed with CGA containing 0 mg/kg (CK), 100 mg/kg (L100), 200 mg/kg (L200), 400 mg/kg
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Isolation and the pathogenicity characterization of Decapod iridescent virus 1 (DIV1) from diseased Macrobrachium nipponense and its activation on host immune response Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-01-23 Lijie Qin, Qieqi Qian, Anting Chen, Yingjie Zhang, Xinzhe Tang, Tianchi Yin, Qun Jiang, Xiaojun Zhang, Xiaojian Gao
The high morbidity and mortality of Macrobrachium nipponense occurred in several farms in China, with cardinal symptoms of slow swimming, loss of appetite, empty of intestine, reddening of the hepatopancreas and gills. The pathogen has been confirmed as Decapod Iridescent Virus 1 (DIV1), namely DIV1-mn, by molecular epidemiology, histopathological examination, TEM observation, challenge experiment
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A novel study of brain microvascular endothelial cells induced by astrocyte conditioned medium for constructing blood brain barrier model in vitro: A promising tool for meningitis of teleost Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-01-23 Yanghui Chen, Yuan Li, Junliang Luo, Zixin Li, Yu Huang, Jia Cai, Dongneng Jiang, Defeng Zhang, Jichang Jian, Jun Qiang, Bei Wang
The blood-brain barrier (BBB) is mainly composed of specialized endothelial cells, which can resist harmful substances, transport nutrients, and maintain the stability of the brain environment. In this study, an endothelial cell line from tilapia (Oreochromis niloticus) named TVEC-01 was successfully established. During the earlier establishment phase of the cell line, the TVEC-01 cells were persistently
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Identification, characterization and the inflammatory regulating effect of NOD1/2 in sturgeon Fish Shellfish Immun. (IF 4.7) Pub Date : 2024-01-26 Defang Chen, Hao Zhu, Lu Lu, Yinqiu Chen, Xin Zhang, Xiaoli Huang, Ping Ouyang, Yi Geng, Zhiqiong Li
As an ancient species with both conservation and commercial value, Sturgeon's inflammatory regulation mechanism is a research point. Nucleotide-binding and oligomerization domain-containing proteins 1 and 2 (NOD1/2) are classical intracellular pattern recognition receptors (PRRs) in immunity of anti-bacterial infection. However, the characterization and function of NOD1/2 in Sturgeon are still unclear