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Post-translational suppression of the high affinity IgE receptor expression on mast cells by an intestinal bacterium Immunobiology (IF 2.788) Pub Date : 2021-01-23 Sakino Fukatsu; Hikari Horinouchi; Shiho Nagata; Risa Kamei; Daichi Tanaka; Wonki Hong; Yui Kazami; Minami Fujimori; Kikuji Itoh; Yoshika Momose; Kazumi Kasakura; Akira Hosono; Shuichi Kaminogawa; Shigemasa Hanazawa; Yusuke Nakanishi; Kyoko Takahashi
Mast cells, which express the high-affinity IgE receptor (FcεRI) on their surface, play a crucial role in inducing allergic inflammation. Since mast cells are activated by crosslinking of FcεRI with IgE and allergens, the cell surface expression level of FcεRI is an important factor in determining the sensitivity to allergens. Recently, the involvement of gut microbiota in the prevalence and regulation
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Glycosylation is required for the neutralizing activity of human IgG1 antibodies against human rabies induced by pre-exposure prophylaxis Immunobiology (IF 2.788) Pub Date : 2021-01-23 Gabriela Koike; Iana Suly Santos Katz; Elaine Raniero Fernandes; Fernanda Guedes; Sandriana Ramos Silva
Rabies lyssavirus (RABV) neutralizing IgG antibodies confer protection after rabies vaccination, although how the RABV-specific antibodies neutralize the virus is still unknown. As changes in the antibody‘s carbohydrate chain can interfere with its effector functions, we compared the glycosylation patterns of both neutralizing and non-neutralizing IgG1 induced by pre-exposure prophylaxis to human rabies
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Immune Induction by adjuvanted Leishmania donovani vaccines against the visceral leishmaniasis in BALB/c mice Immunobiology (IF 2.788) Pub Date : 2021-01-23 Deepak Kumar Goyal; Poonam Keshav; Sukhbir Kaur
Visceral leishmaniasis (VL) is a neglected tropical disease caused by Leishmania donovani or Leishmania infantum. Currently, the patients are treated with chemotherapeutic drugs; however, their toxicity limits their use. It would be desirable to develop a vaccine against this infection. In this study, we assessed the efficacy of different vaccine formulations at variable time points. Heat-killed (HK)
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Exposure of pregnant rats to staphylococcal enterotoxin B increases offspring splenic Treg number and function via decreasing FoxP3 methylation Immunobiology (IF 2.788) Pub Date : 2021-01-23 Shu-xian Gao; Chao Sun; Yu-ting Zhu; Jia-bao Zhao; Jing Sun; Ping Zhou; Hao-yuan Jiang; Ying-ao Fan; Li Wei; Tao Zhang; Jun-chang Guan
Staphylococcus aureus is an infectious pathogen that is relatively common, but that can cause severe disease in pregnant women and their fetuses. We previously demonstrated that exposing pregnant rats to staphylococcal enterotoxin B (SEB) altered splenic CD4/CD8 T cell frequencies in their offspring. Whether prenatal SEB exposure impacts Tregs in these offspring, however, remains to be determined.
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The level of thymic stromal lymphopoietin and its gene polymorphism are associated with rheumatoid arthritis Immunobiology (IF 2.788) Pub Date : 2021-01-23 Weifeng Wang; Qiudan Chen; Jianguang Wang; Tianming Li; Guanglei Song; Kun Tao; Junjian Liu; Zhong Wu
Objective To study the correlation between TSLP gene SNPs and RA in a Han Chinese population. Methods The genotypes of TSLP genes rs11466749, rs11466750 and rs10073816 among 197 RA patients and 197 controls were analysed by direct sequencing. ELISA was used to detect the plasma TSLP level. Logistic regression analysis was also conducted to identify risk factors for RA. Results The rs11466749 locus
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Effects of TNF-α on autophagy of rheumatoid arthritis fibroblast-like synoviocytes and regulation of the NF-κB signaling pathway Immunobiology (IF 2.788) Pub Date : 2021-01-23 Yu Wang; Wei Gao
Rheumatoid arthritis (RA) is a common chronic autoimmune disease, which seriously harms human health. The hyperplastic growth of fibroblast-like synoviocytes (FLSs) plays a key role in the pathogenesis of RA. However, the pathogenesis of RA remains unclear. In this experiment, we confirmed that Tumor necrosis factor alpha (TNF-α) could activate the autophagy of RA-FLSs. 3-Methyladenine (3-MA) and Chloroquine
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What do we know about the antibody responses to SARS-CoV-2? Immunobiology (IF 2.788) Pub Date : 2021-01-23 Francisco Alejandro Lagunas-Rangel; Venice Chávez-Valencia
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread worldwide causing a pandemic with millions of infected people and deaths. Currently, the scientific community is working hard to develop a specific vaccine or treatment. However, since antibody production is an important part of the adaptive immune response, to develop vaccines and therapies, we must understand the antibody response
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Immunoinformatics approach for a novel multi-epitope subunit vaccine design against various subtypes of Influenza A virus Immunobiology (IF 2.788) Pub Date : 2021-01-21 Shipra Sharma; Vibha Kumari; Bajarang Kumbhar; Amrita Mukherjee; Ruchika Pandey; Kiran Kondabagil
Vaccination is the best strategy for the control and prevention of contagious diseases caused by Influenza A viruses. Extraordinary genetic variability and continual evolvability are responsible for the virus having survival and adaptation to host cell immune response, thus rendering the current influenza vaccines with suboptimal effectiveness. Therefore, in the present study, using a novel immunoinformatics
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Expression profiles of HMGB1 on B-CLL related leukocytes contribute to prediction of relapse Immunobiology (IF 2.788) Pub Date : 2020-12-16 Joerg Schmohl; Thomas Guenther; Wishnu Sutanto; Friedhelm Schuster; Tanja Kroell; Amely Hartmann; Helmut Salih; Oliver Stoetzer; H. Schmetzer
Background The High Mobility Group Box 1 (HMGB1) is a nuclear protein that is frequently overexpressed in hematologic diseases and might be of relevance in immunogenic cancer control thus correlating with patients’ (pts.) prognosis in diseases such as acute myeloid, acute lymphatic and chronic lymphocytic leukemia. Materials and methods Expression profiles of blasts from AML (n = 21), ALL (n = 16)
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BCG immunomodulation: From the ‘hygiene hypothesis’ to COVID-19 Immunobiology (IF 2.788) Pub Date : 2020-12-24 Aaron J. Moulson; Yossef Av-Gay
The century-old tuberculosis vaccine BCG has been the focus of renewed interest due to its well-documented ability to protect against various non-TB pathogens. Much of these broad spectrum protective effects are attributed to trained immunity, the epigenetic and metabolic reprogramming of innate immune cells. As BCG vaccine is safe, cheap, widely available, amendable to use as a recombinant vector
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Association of interleukin-17 gene polymorphisms with the onset of Rheumatoid Arthritis Immunobiology (IF 2.788) Pub Date : 2020-12-03 Amna Amin; Nadeem Sheikh; Maryam Mukhtar; Tayyaba Saleem; Tasleem Akhtar; Naz Fatima; Rabia Mehmood
Rheumatoid Arthritis (RA) is an autoimmune disorder where multiple cytokines including IL-17A and IL-17F produced by T helper cell 17 (Th17), contribute to its pathogenesis. By initiating inflammatory responses in joints Th17 act as pathogenic driver leading to bone and cartilage destruction in RA patients. Hence, the planned study was aimed to estimate IL-17 gene polymorphism association with RA susceptibility
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PPE2 protein of Mycobacterium tuberculosis affects myeloid hematopoiesis in mice Immunobiology (IF 2.788) Pub Date : 2020-12-03 Ravi Pal; Sangita Mukhopadhyay
Irregularity in hematopoiesis is noted in humans during tuberculosis. However, influence of mycobacterial protein(s) on bone marrow hematopoiesis is not fully understood. In this study, we have demonstrated the role of a mycobacterial protein, PPE2 (Rv0256c) in suppressing hematopoiesis during infection. PPE2 belongs to PPE (proline-proline-glutamine) family of mycobacterial proteins which are well
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The MHC-II antigen presentation machinery and B7 checkpoint ligands display distinctive patterns correlated with acute myeloid leukaemias blast cells HLA-DR expression Immunobiology (IF 2.788) Pub Date : 2020-12-03 Ion Antohe; Mariana Pavel Tanasa; Angela Dăscălescu; Cătălin Dănăilă; Amalia Titieanu; Mihaela Zlei; Iuliu Ivanov; Adriana Sireteanu; Petru Cianga
Acute Myeloid Leukaemia (AML) is a neoplasia characterised by rapid proliferation and an increased rate of relapses. The AML blasts display features of antigen-presenting cells (APC), and thus can directly modulate the anti-tumour T cell responses. The bone marrow of a group consisting of 30 newly diagnosed patients and four healthy donors (HD) was investigated for the expression of HLA-DR, several
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Benznidazole: Hero or villain of cellular immune response in chronic Chagas disease patients? Immunobiology (IF 2.788) Pub Date : 2020-12-04 Ana K.A. Soares; Patrícia A.F. Neves; Amanda V. Nascimento; Artur A.M. Esmeraldo; Leyllane R. Moreira; Taciana M.M. Higino; Regina C.B.Q. Figueiredo; Maria G.A.M. Cavalcanti; Sílvia M. Martins; Cristina Carrazone; Wilson O. Júnior; Yara M. Gomes; Virginia M.B. Lorena
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α9α10 nicotinic acetylcholine receptors regulate murine bone marrow granulocyte functions Immunobiology (IF 2.788) Pub Date : 2020-12-03 Valentina G. Safronova; Catherine A. Vulfius; Maxim E. Astashev; Irina V. Tikhonova; Dmitriy A. Serov; Elina A. Jirova; Ekaterina V. Pershina; Dmitry A. Senko; Maxim N. Zhmak; Igor E. Kasheverov; Victor I. Tsetlin
Polymorphonuclear neutrophilic granulocytes (PMNs) are extremely important in defense of the organism against infections and in inflammatory processes including neuroinflammation and pain sensation. Different subtypes of nicotinic acetylcholine receptors (nAChRs) are involved in modulation of PMN activities. Earlier we determined expression of α2-7, α9, β3, β4 subunits and regulatory role of α7 and
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Decrease in naïve T cell production due to HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) development Immunobiology (IF 2.788) Pub Date : 2020-12-03 Jéssica Antonia Nunes Gomes; George Alberto da Silva Dias; Satomi Fujihara; Gilberto Toshimitsu Yoshikawa; Roberta Vilela Lopes Koyama; Rita Catarina Medeiros Sousa; Juarez Antônio Simões Quaresma; Hellen Thais Fuzii
Human T-lymphocytic virus 1 (HTLV-1) is mainly associated with adult T-cell leukemia/lymphoma (ATLL) and HTLV-1–associated myelopathy/tropical spastic paraparesis (HAM/TSP). Patients with HAM/TSP exhibit significant changes in their immune response, and HTLV-1 infection can interfere in cytokine production and perhaps in T cell production. The aims of this study were to evaluate thymic function in
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Advances in immunotherapy for the treatment of spinal cord injury Immunobiology (IF 2.788) Pub Date : 2020-11-24 Abdullah Al Mamun; Ilma Monalisa; Khadija Tul Kubra; Afroza Akter; Jaheda Akter; Tamanna Sarker; Fahad Munir; Yanqing Wu; Chang Jia; Masuma Afrin Taniya; Jian Xiao
Spinal cord injury (SCI) is a leading cause of morbidity and disability in the world. Over the past few decades, the exact molecular mechanisms describing secondary, persistent injuries, as well as primary and transient injuries, have attracted massive attention to the clinicians and researchers. Recent investigations have distinctly shown the critical roles of innate and adaptive immune responses
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Cytotoxic activity of human dendritic cells induces RIPK1-dependent cell death Immunobiology (IF 2.788) Pub Date : 2020-11-23 Zsófia Varga; Evelin Rácz; Anett Mázló; Mónika Korodi; Anikó Szabó; Tamás Molnár; Árpád Szöőr; Zoltán Veréb; Attila Bácsi; Gábor Koncz
Dendritic cells (DCs), as potent phagocytes engulf dead cells and present peptide fragments of tumor antigens or pathogens derived from infected cells to naïve CD8+ T-lymphocytes. Dendritic cells can also induce apoptosis in target cells, thus getting an opportunity to sample their microenvironment. Here, we present that the supernatants of LPS- or CL075-activated DCs induced cell death in different
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Effect of pathogenic crystals on the production of pro- and anti-inflammatory cytokines by different leukocyte populations Immunobiology (IF 2.788) Pub Date : 2020-11-26 Anna Scanu; Francesca Oliviero; Roberto Luisetto; Roberta Ramonda; Andrea Doria; Leonardo Punzi; Jean-Michel Dayer
Purpose To evaluate cytokine production in vitro by different types of leukocytes stimulated with monosodium urate (MSU), calcium pyrophosphate (CPP) and basic calcium phosphate (BCP) crystals. Material and methods Polymorphonuclear cells (PMN), monocytes and lymphocytes, isolated from healthy volunteer blood, were stimulated for different time periods with increasing MSU, CPP or BCP crystal concentrations
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PAF-R on activated T cells: Role in the IL-23/Th17 pathway and relevance to multiple sclerosis Immunobiology (IF 2.788) Pub Date : 2020-11-13 Angela Midgley; Dina Barakat; Manjit Braitch; Calen Nichols; Mihailo Nebozhyn; Laura J. Edwards; Susan C. Fox; Bruno Gran; R. Adrian Robins; Louise C. Showe; Cris S. Constantinescu
IL-23 is a potent stimulus for Th17 cells. These cells have a distinct developmental pathway from Th1 cells induced by IL-12 and are implicated in autoimmune and inflammatory disorders including multiple sclerosis (MS). TGF-β, IL-6, and IL-1, the transcriptional regulator RORγt (RORC) and IL-23 are implicated in Th17 development and maintenance. In human polyclonally activated T cells, IL-23 enhances
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Modulatory effect of rapamycin and tacrolimus on monocyte-derived dendritic cells phenotype and function Immunobiology (IF 2.788) Pub Date : 2020-11-23 Geraldine Dahlqvist; Sarah Renaud; Clement Barjon; Anthony Lefebvre; Lynda Aoudjehane; Yves Horsmans; Nadira Delhem; Filomena Conti
Background Immunosuppressive-drugs are needed after solid organ transplantation to prevent allograft rejection but induce severe side effects. Understanding the alloimmune response is critical to modulate it and to achieve graft operational tolerance. The role of regulatory T cells and tolerogenic dendritic cells (Tol-DCs) is undoubtedly essential in tolerance induction. Tacrolimus is considered as
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IL-27 regulates IL-4-induced chemokine production in human bronchial epithelial cells Immunobiology (IF 2.788) Pub Date : 2020-11-26 Aline Beatriz Mahler Pereira; Jhony Robison de Oliveira; Maxelle Martins Teixeira; Paulo Roberto da Silva; Virmondes Rodrigues Junior; Alexandre de Paula Rogerio
IL-4 coordinates the Th2-type immune response in inflammatory diseases such as asthma. IL-27 can inhibit the development of both Th2 and Th1 cells. However, IL-27 can also drive naïve T cells to differentiate toward the Th1 phenotype. In this study, we investigated the effects of IL-27 on the activation of IL-4-induced human bronchial epithelial cells (BEAS-2B). Compared to controls, both IL-4 and
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Myeloid arginase-1 controls excessive inflammation and modulates T cell responses in Pseudomonas aeruginosa pneumonia Immunobiology (IF 2.788) Pub Date : 2020-11-24 Dalia Haydar; Rene Gonzalez; Beth A. Garvy; Sylvie Garneau-Tsodikova; Nishad Thamban Chandrika; Therese J. Bocklage; David J. Feola
Regulatory properties of macrophages associated with alternative activation serve to limit the exaggerated inflammatory response during pneumonia caused by Pseudomonas aeruginosa infection. Arginase-1 is an important effector of these macrophages believed to play an essential role in decreasing injury and promoting repair. We investigated the role of arginase-1 in the control of inflammatory immune
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Effect of lipopolysaccharide structure on functional response of whole blood cells Immunobiology (IF 2.788) Pub Date : 2020-11-23 Svetlana V. Zubova; Sergey V. Grachev; Isabella R. Prokhorenko
Lipopolysaccharides (LPSs) induce a wide spectrum of functional activities after interaction with blood cells. Effect of structure of toxic LPS from S- and Re-chemotypes of E. coli and/or non-toxic LPS of Rhodobacter capsulatus PG (R. caps.) on activation of neutrophils and monocytes of human whole blood were studied, particularly, expression of TLR4, CD14 and CD11b receptors, phagocytosis of BioParticles
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Estrogen receptors in human bladder cells regulate innate cytokine responses to differentially modulate uropathogenic E. coli colonization Immunobiology (IF 2.788) Pub Date : 2020-11-04 Ayantika Sen; Anil Kaul; Rashmi Kaul
The bladder epithelial cells elicit robust innate immune responses against urinary tract infections (UTIs) for preventing the bacterial colonization. Physiological fluctuations in circulating estrogen levels in women increase the susceptibility to UTI pathogenesis, often resulting in adverse health outcomes. Dr adhesin bearing Escherichia coli (Dr E. coli) cause recurrent UTIs in menopausal women and
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Study on the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 −/−) and wild-type (WT) mice Immunobiology (IF 2.788) Pub Date : 2020-11-12 Darab Ghadimi; Michael de Vrese; Michael Ebsen; Christoph Röcken; Sven Olaf Frahm; Janine Zahlten; Regina Fölster-Holst; Knut J. Heller; Wilhelm Bockelmann
Background and Aims Pglyrp3 is a bactericidal innate immunity protein known to sustain the habitual gut microbiome and protect against experimental colitis. Intestinal inflammation and metaflammation are commonly associated with a marked reduction of commensal bifidobacteria. Whether Pglyrp3 and bifidobacteria interact synergistically or additively to alleviate metaflammation is unknown. We investigated
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SARS-CoV-2 attachment to host cells is possibly mediated via RGD-integrin interaction in a calcium-dependent manner and suggests pulmonary EDTA chelation therapy as a novel treatment for COVID 19 Immunobiology (IF 2.788) Pub Date : 2020-11-05 Tikam Chand Dakal
SARS-CoV-2 is a highly contagious virus that has caused serious health crisis world-wide resulting into a pandemic situation. As per the literature, the SARS-CoV-2 is known to exploit humanACE2 receptors (similar toprevious SARS-CoV-1) for gaining entry into the host cell for invasion, infection, multiplication and pathogenesis. However, considering the higher infectivity of SARS-CoV-2 along with the
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Hypothyroidism impairs the host immune response during the acute phase of Chagas disease Immunobiology (IF 2.788) Pub Date : 2020-11-04 Pedro Alexandre Sampaio; Amanda Goulart; Vânia Brazão; Naira Ferreira Anchieta; Maiara Voltarelli Providello; Gisele Bulhões Portapilla; Andressa Duarte; Jefferson Luiz da Silva; José Clóvis do Prado Júnior
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Successful perioperative management of three patients with hereditary angioedema without C1 esterase inhibitor therapy: A developing country perspective Immunobiology (IF 2.788) Pub Date : 2020-11-03 Ankur Kumar Jindal; Ankita Singh; Gummadi Anjani; Anit Kaur; Manojkumar Jaiswal; Seema Chopra; Uttam Saini; Shalvi Mahajan; Amit Rawat; Surjit Singh; Hilary Longhurst
Background Hereditary angioedema (HAE) is a rare inherited disorder characterized by sudden and unpredictable appearance of swelling. Surgical procedures, even minor ones, are known to precipitate an attack in these patients. C1 esterase inhibitor (C1-INH) therapy may be effective for short term prophylaxis in such situations. However, there is limited experience with short term prophylaxis in countries
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Roles of protein ubiquitination in inflammatory bowel disease Immunobiology (IF 2.788) Pub Date : 2020-11-04 Yue Xiao; Qi Huang; Zherui Wu; Weilin Chen
Inflammatory bowel disease (IBD) including Crohn’s disease (CD) and ulcerative colitis (UC) seriously affects the quality of life for patients. The pathogenesis of IBD contains the environmental, host genetic and epigenetic factors. In recent years, the studies of protein ubiquitination, an important protein post-translational modification as an epigenetic factor, have emerged in the pathogenesis and
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IL-33 synergistically promotes the proliferation of lung cancer cells in vitro by inducing antibacterial peptide LL-37 and proinflammatory cytokines in macrophages Immunobiology (IF 2.788) Pub Date : 2020-11-03 Yinting Jiang; Hongyi Liao; Xuemei Zhang; Sijia Cao; Xuexue Hu; Zihan Yang; Yuting Fang; Hong Wang
Lung cancer is the primary cause of cancer-related deaths, and the persistent inflammation is inextricably linked with the lung cancer tumorigenesis. Pro-inflammatory cytokine interleukin-33 (IL-33) is able to serve as a potent modulator of cancer. Mounting evidence indicates IL-33 has significant effect on lung cancer progression by regulating host immune response, but the current opinions about the
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An association of iNKT+/CD3+/CD161+ lymphocytes in ovarian cancer tissue with CA125 serum concentration Immunobiology (IF 2.788) Pub Date : 2020-08-28 Izabela Winkler; Justyna Woś; Agnieszka Bojarska-Junak; Andrzej Semczuk; Tomasz Rechberger; Włodzimierz Baranowski; Ewa Markut-Miotła; Jacek Tabarkiewicz; Ewa Wolińska; Maciej Skrzypczak
The purpose of this study was to investigate the association of iNKT (human invariant natural killer T) cells with the key marker of ovarian cancer (OC) - CA125 (cancer antigen125) in serum. The study reports the assessment of iNKT cells in peripheral blood and tissue of benign and borderline ovarian tumors (BOTs) and in the advanced-stage ovarian cancer. The study groups were as follows: 25 women
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A method to differentiate chicken monocytes into macrophages with proinflammatory properties Immunobiology (IF 2.788) Pub Date : 2020-08-27 Lianci Peng; Robin H.G.A van den Biggelaar; Christine A. Jansen; Henk P. Haagsman; Edwin J.A. Veldhuizen
Macrophages are part of the first line of defense against invading pathogens. In mammals, the in vitro culture of macrophages from blood monocytes or bone marrow cells is well established, including culturing conditions to differentiate them towards M1 or M2-like macrophages. In chicken, monocyte-derived macrophages have been used in several studies, but there is no uniform protocol or actual characterization
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Anti-neutrophil antibodies (anti-MPO-ANCAs) are associated with poor prognosis in breast cancer patients Immunobiology (IF 2.788) Pub Date : 2020-09-07 Thalita Basso Scandolara; Janaína Carla da Silva; Jéssica Malanowski; Janoário Athanázio de Oliveira; Daniel Rech; Carolina Panis
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Absence of complement factor H reduces physical performance in C57BL6 mice. Immunobiology (IF 2.788) Pub Date : 2020-08-28 Kenneth L Seldeen,Ramkumar Thiyagarajan,Yonas Redae,Alexander Jacob,Bruce R Troen,Richard J Quigg,Jessy J Alexander
Complement (C) system is a double edge sword acting as the first line of defense on the one hand and causing aggravation of disease on the other. C activation when unregulated affects different organs including muscle regeneration. However, the effect of factor H (FH), a critical regulator of the alternative C pathway in muscle remains to be studied. FH deficiency results in excessive C activation
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The role of macrophages type 2 and T-regs in immune checkpoint inhibitor related adverse events. Immunobiology (IF 2.788) Pub Date : 2020-08-21 Daniela Mihic-Probst,Michael Reinehr,Susanne Dettwiler,Isabel Kolm,Christian Britschgi,Ken Kudura,Ewerton Marques Maggio,Daniela Lenggenhager,Elisabeth J Rushing
Immune checkpoint inhibitory (ICI) therapy represents a novel approach in a variety of cancers, with impressive survival benefit. With ICIs, however, a new spectrum of immune related adverse events (irAE) including life threatening hypohysitis has emerged. This autopsy study aimed to investigate inflammatory cells, PD-1 and PD-L1 expression in cases of patients who developed hypophysitis and involvement
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Transient Foxp3(+) regulatory T-cell depletion enhances protective Th1/Th17 immune response in murine sporotrichosis caused by Sporothrix schenckii. Immunobiology (IF 2.788) Pub Date : 2020-08-02 Alexander Batista-Duharte,Damiana Téllez-Martínez,Cleverton Roberto de Andrade,Marisa Campos Polesi,Deivys Leandro Portuondo,Iracilda Zeppone Carlos
The role of regulatory T cells (Tregs) on protective immunity in fungal infections, is controversial. Sporotrichosis is an emerging and worldwide-distributed subcutaneous mycosis caused by various related thermodimorphic fungi of the genus Sporothrix. Previously, we showed an elevated percent of Tregs around 21 days post-infection (dpi) in C57BL/6 mice infected with either Sporothrix schenckii or Sporothrix
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The TGFB1 -509C/T polymorphism and elevated TGF-β1 levels are associated with chronic hepatitis C and cirrhosis. Immunobiology (IF 2.788) Pub Date : 2020-08-13 William Botelho de Brito,Maria Alice Freitas Queiroz,Ednelza da Silva Graça Amoras,Sandra Souza Lima,Simone Regina Souza da Silva Conde,Eduardo José Melo Dos Santos,Izaura Maria Vieira Cayres-Vallinoto,Ricardo Ishak,Antonio Carlos Rosário Vallinoto
The IFN-γ and TGF-β1 cytokines perform antagonistic activities in the immune response, and polymorphisms in these genes may induce changes in their plasma levels and influence the course of chronic Hepacivirus C (HCV) infection. The present study evaluated the IFNG +874A/T and TGFB1 -509 C/T polymorphisms in 99 samples from patients with chronic hepatitis C and in 300 samples from healthy donors, and
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B-lymphocyte deficiency and recurrent respiratory infections in a 6-month-old female infant with mosaic monosomy 7. Immunobiology (IF 2.788) Pub Date : 2020-08-12 Kailin Mai,Xiaowen Chen,Chunli Wang,Shangzhi Wu,Liying Yang,Zhanhang Huang,Guanglin Zhang,Victor Wei Zhang,Jing Wang,Dehui Chen
Monosomy 7 is generally considered as an acquired cytogenetic abnormality within hematopoietic cells, and indicates an especially high risk of progression to bone marrow failure, myelodysplastic syndrome (MDS) or juvenile myelomonocytic leukemia (JMML). We report a case of a 6-month-old female infant with mosaic monosomy 7 who presented with clinical and laboratory evidences of immunodeficiency. The
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TEAD4 transcriptional regulates SERPINB3/4 and affect crosstalk between keratinocytes and T cells in psoriasis. Immunobiology (IF 2.788) Pub Date : 2020-08-14 Cuimin Ren,Qiang Liu,Yaohui Ma,Aixue Wang,Yun Yang,Dahu Wang
Psoriasis is a common chronic inflammatory disease with the prevalence rate of approximately 1–3 %. Currently, it is generally believed that the pathogenesis of psoriasis is a T-cell immune-mediated skin disease mediated by multiple genes and factors, and the interaction between keratinocytes and T cells. TEA domain family member 4 (TEAD4) is a transcription factor which regulates the expression of
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Soluble cluster of differentiation 74 regulates lung inflammation through the nuclear factor-κB signaling pathway. Immunobiology (IF 2.788) Pub Date : 2020-08-13 Banghui Zhu,Guosheng Wu,Chen Wang,Yongqiang Xiao,Jian Jin,Kangan Wang,Yong Jiang,Yu Sun,Daofeng Ben,Zhaofan Xia
The soluble form of the migration inhibitory factor receptor cluster of differentiation 74 (sCD74) has previously been shown to be elevated during the development of acute lung injury (ALI) in mice. However, the biological role of increased sCD74 in ALI remans poorly understood. Synthesized recombinant sCD74 protein was administered to mice intratracheally. Pro-inflammatory genes in lung tissue and
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Host-pathogen interaction in COVID-19: Pathogenesis, potential therapeutics and vaccination strategies Immunobiology (IF 2.788) Pub Date : 2020-08-19 Praveen Mathews Varghese; Anthony G. Tsolaki; Hadida Yasmin; Abhishek Shastri; Janez Ferluga; Manu Vatish; Taruna Madan; Uday Kishore
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Anti-complement factor I antibody associated atypical hemolytic uremic syndrome - A new insight for future perspective! Immunobiology (IF 2.788) Pub Date : 2020-08-11 Srinivasavaradan Govindarajan,Amit Rawat,Raja Ramachandran,Rekha Hans,Lesa Dawman,Karalanglin Tiewsoh
Atypical hemolytic uremic syndrome (aHUS) is caused mainly by complement dysregulation. Although various defects in the complement system explaining pathophysiology have been described in recent years, the etiology still remains unclear in about thirty percent of cases. In exploring other causes, similar to anti- complement factor H (anti-CFH) antibody associated HUS, we hypothesized that anti-complement
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The establishment of humanized IL-4/IL-4RA mouse model by gene editing and efficacy evaluation. Immunobiology (IF 2.788) Pub Date : 2020-08-03 Peili Yan,Youhong Su,Chengzhang Shang,Xiaofei Zhou,Yi Yang,Wenqian An,Wenlin An,Changyuan Yu,Shihui Wang
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Estrogen signaling differentially alters iron metabolism in monocytes in an Interleukin 6-dependent manner. Immunobiology (IF 2.788) Pub Date : 2020-08-02 Khuloud Bajbouj,Jasmin Shafarin,Jibran Sualeh Muhammad,Amjad Ali,Hema Unnikannan,Betoul Suleiman,Nour Al-Jabi,Kiran Menon,Mawieh Hamad
The ability of monocytes to release or sequester iron affects their role in cancer and inflammation. Previous work has shown that while IL-6 upregulates hepcidin synthesis and enhances iron sequestration, E2 reduces hepcidin synthesis and increases iron release. Given that E2 upregulates IL-6 production in monocytes, it is likely that the exact effect of E2 on iron metabolism in monocytes is shaped
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Myeloperoxidase and lactoferrin expression in semen fluid: Novel markers of male infertility risk? Immunobiology (IF 2.788) Pub Date : 2020-08-05 Claudia Omes,Mara De Amici,Veronica Tomasoni,Federica Todaro,Cristina Torre,Rossella E Nappi
Research Question Infections and/or inflammation processes of male genital tract are highly prevalent and often associated with risk of infertility. These conditions represent a possible cause of leukocytospermia, which is still under debate. Leukocytes are key-factors to reactive oxygen species (ROS) production and the increase of ROS in semen fluid is associated with the worsening of semen parameters
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Endothelial injury and thrombotic microangiopathy in COVID-19: Treatment with the lectin-pathway inhibitor narsoplimab. Immunobiology (IF 2.788) Pub Date : 2020-08-09 Alessandro Rambaldi,Giuseppe Gritti,Maria Caterina Micò,Marco Frigeni,Gianmaria Borleri,Anna Salvi,Francesco Landi,Chiara Pavoni,Aurelio Sonzogni,Andrea Gianatti,Francesca Binda,Stefano Fagiuoli,Fabiano Di Marco,Luca Lorini,Giuseppe Remuzzi,Steve Whitaker,Gregory Demopulos
In COVID-19, acute respiratory distress syndrome (ARDS) and thrombotic events are frequent, life-threatening complications. Autopsies commonly show arterial thrombosis and severe endothelial damage. Endothelial damage, which can play an early and central pathogenic role in ARDS and thrombosis, activates the lectin pathway of complement. Mannan-binding lectin-associated serine protease-2 (MASP-2), the
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Mouse bone marrow-derived mesenchymal stem cells acquire immunogenicity concurrent with differentiation to insulin-producing cells. Immunobiology (IF 2.788) Pub Date : 2020-07-31 Nabiallah Mohammadi,Alireza Mardomi,Hadi Hassannia,Seyed Ehsan Enderami,Hossein Ranjbaran,Alireza Rafiei,Saeid Abediankenari
Background Although mesenchymal stem cells (MSCs) are regarded as immune-elusive and even immunosuppressive, recent evidence suggests that allogeneic immune response might is inevitable in the case of some lineages differentiated from MSCs. Regarding the importance of allogeneic IPCs and MSCs in pre-clinical and clinical studies, the present study aimed to investigate the possible changes in the immunogenicity
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Different roles of intracellular and extracellular reactive oxygen species of neutrophils in type 2 diabetic mice with invasive aspergillosis. Immunobiology (IF 2.788) Pub Date : 2020-08-01 Xianghua Xu,Chu Xia,Yi Huang
Diabetic patients have an increased risk of invasive aspergillosis (IA), but the mechanism is still unclear. Reactive oxygen species (ROS) produced by neutrophils play a key role in defense against Aspergillus infection. Since diabetes mellitus affects the production of ROS from neutrophils, the purpose of this study is to investigate whether this effect is related to the susceptibility of diabetic
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Adiponectin and leptin levels in idiopathic pulmonary fibrosis: A new method for BAL and serum assessment. Immunobiology (IF 2.788) Pub Date : 2020-08-01 Miriana d'Alessandro,Laura Bergantini,Rosa Metella Refini,Paolo Cameli,Felice Perillo,Claudia Landi,Fiorella Icorne,Anna Perrone,Piersante Sestini,Francesco Bonella,Elena Bargagli
Adipokines (APN) are mainly secreted by adipocytes, macrophages and various other cells, along with their role in the regulation and mediation of inflammatory responses. APN is almost exclusively synthesized by adipocytes and regulated by peroxisome proliferator-activated receptor γ (PPARγ) that is involved in the epithelial-mesenchymal transition, linked lung fibrosis. Leptin is involved in acute
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Fungal β-glucans and mannan stimulate peripheral blood mononuclear cells to cytokine production in Syk-dependent manner. Immunobiology (IF 2.788) Pub Date : 2020-07-28 Elżbieta Kozłowska,Ewa Brzezińska-Błaszczyk,Paweł Rasmus,Paulina Żelechowska
There is evidence that major components of the fungi cell wall not only define fungal properties and survival but also are responsible for their biological activities. Some data indicate that structural components of the fungal cell wall exert stimulatory/modulatory effects on immunocompetent cells acting as pathogen-associated molecular patterns (PAMPs). Fungal components can influence the activity
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In vitro immunobiological assays of methotrexate-stearic acid conjugate in human PBMCs. Immunobiology (IF 2.788) Pub Date : 2020-07-06 Nadia Fattahi,Abbas Bahari,Ali Ramazani,Davoud Koolivand
In the present research, a lipophilic methotrexate (MTX) prodrug was developed by covalent conjugating of MTX to stearic acid (SA) as a lipid moiety via amid bond. The structure of synthesized conjugate, MTX-SA, was confirmed by IR and NMR spectra. To evaluate inflammatory response of MTX-SA conjugate and MTX, human PBMCs were isolated and exposed to 50, 500 and 5000 nM of MTX-SA conjugate and free
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Exploring Kawasaki disease-specific hub genes revealing a striking similarity of expression profile to bacterial infections using weighted gene co-expression network analysis (WGCNA) and co-expression modules identification tool (CEMiTool): An integrated bioinformatics and experimental study. Immunobiology (IF 2.788) Pub Date : 2020-07-06 Sajjad Esmaeili,Amir Mehrgou,Naser Kakavandi,Yazdan Rahmati
Kawasaki disease (KD) has been declared a rare idiopathic condition for a long time. The children age less than five years, as the most susceptible group, are at risk of this disease. Since the cause of the disease is unknown, this study was designed to investigate the cause of KD. We applied metaDE and WGCNA packages in order to perform a meta-analysis and identify network modules of co-expressed
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Identification of a peptide targeting CD56. Immunobiology (IF 2.788) Pub Date : 2020-07-06 Hongxing Huang,Ying Liu,Xiaoming Ouyang,Hua Wang,Yan Zhang
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RNASeq analysis reveals upregulation of complement C3 in the offspring gut following prenatal stress in mice. Immunobiology (IF 2.788) Pub Date : 2020-07-06 Venkata A Yeramilli,Kyle M Brawner,David K Crossman,Scott R Barnum,Colin A Martin
Dysregulated activation of inflammatory signaling by the immature neonatal immune system could lead to the development of many pediatric diseases including necrotizing enterocolitis (NEC). While the mechanism(s) of pathogenesis is unknown, NEC is believed to have multifactorial causes. Microbial dysbiosis and intestinal immaturity have been implicated as potential triggers for this disease. We hypothesized
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The role of IL-17A/IL-17RA and lung injuries in children with lethal non-pandemic acute viral pneumonia. Immunobiology (IF 2.788) Pub Date : 2020-07-06 Marina Luise Viola Azevedo,Mineia Alessandra Scaranello Malaquias,Caroline Busatta Vaz de Paula,Cleber Machado de Souza,Victor Horácio Costa Júnior,Sonia Mara Raboni,Renata Halila,Giuliana Rosendo,Priscilla Gozzo,Leticia Arianne Panini do Carmo,Plínio Cézar Neto,Seigo Nagashima,Lucia de Noronha
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Paracoccidioides brasiliensis infection increases regulatory T cell counts in female C57BL/6 mice infected via two distinct routes. Immunobiology (IF 2.788) Pub Date : 2020-05-31 Gabriela Peron,Janine Oliveira,Livia de Lima Thomaz,Amanda Pires Bonfanti,Rodolfo Thomé,Catarina Rapôso,Liana M Cardoso Verinaud
Studies that show an overview of the peripheral immune response in a model of Paracoccidioides brasiliensis (Pb) infection in females are scarce in the literature. We sought to characterize the innate and adaptive immune responses in female C57BL/6 mice infected with Pb through two distinct routes of administration, intranasal and intravenous. In addition to the lung, P. brasiliensis yeast cells were
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Cellular immune response of asthmatic children in the presence of anti-Ascaris antibody. Immunobiology (IF 2.788) Pub Date : 2020-06-20 Cassia Nóbrega,Wheverton Nascimento,Virgínia Lorena,Décio Medeiros,Vláudia Costa,Mônica Albuquerque,Constança Barbosa,Dirceu Solé,Emanuel Sarinho,Valdênia Souza
The presence of anti-Ascaris (anti-Asc) immunoglobin isotypes alters the risk of allergic asthma. In this study, we analyzed the relationships between serum levels of anti-Asc IgE, IgG1, and IgG4, without concurrent infection by the parasite, and the presence of asthma. We measured cytokine levels from Th1, Th2, and Th17 profiles. Children aged 2–14 years old, asthmatics (n = 64), and non-asthmatics