-
Developing evidence-based guidance for the treatment of dry eye disease with artificial tear supplements: A six-month multicentre, double-masked randomised controlled trial Ocul. Surf. (IF 12.336) Pub Date : 2021-01-02 Jennifer P. Craig; Alex Muntz; Michael T.M. Wang; Doerte Luensmann; Jacqueline Tan; Sonia Trave Huarte; Ally L. Xue; Lyndon Jones; Mark D.P. Willcox; James S. Wolffsohn
Purpose To assess the six-month therapeutic profiles of lipid and non-lipid-based artificial tear supplements in managing dry eye disease (DED). Methods Ninety-nine participants fulfilling the TFOS DEWS II diagnostic criteria for DED (64% females; mean ± SD age, 44 ± 16 years) were enrolled in a prospective, multicentre, double-masked, parallel group, randomised controlled trial. Participants instilled
-
A systematic review of cellular therapies for the treatment of limbal stem cell deficiency affecting one or both eyes Ocul. Surf. (IF 12.336) Pub Date : 2021-01-04 F.C. Figueiredo; J.M. Glanville; M. Arber; E. Carr; G. Rydevik; J. Hogg; A. Okonkwo; G. Figueiredo; M. Lako; F. Whiter; K. Wilson
Purpose This systematic review (SR) assessed the efficacy, safety and cost-effectiveness of cell-based therapy to manage limbal stem cell deficiency (LSCD), a sight-threatening orphan condition most frequently associated with severe chemical or thermal burns. LSCD has historically been treated by transplanting limbal tissue. In 1997, a new treatment, cultured limbal epithelial autografts, was described
-
Low-dose naltrexone is effective and well-tolerated for modulating symptoms in patients with neuropathic corneal pain Ocul. Surf. (IF 12.336) Pub Date : 2021-01-12 Gabriela Dieckmann; M. Cuneyt Ozmen; Stephanie M. Cox; Ryan C. Engert; Pedram Hamrah
Purpose Neuropathic corneal pain (NCP) is caused by damage or disease of the somatosensory nervous system that innervates the cornea and presents with symptoms of pain or persistent unpleasant sensations, such as burning, dryness, or light sensitivity. This retrospective study aims to assess the efficacy and tolerability of low-dose naltrexone (LDN) in refractory NCP patients. Methods Fifty-nine NCP
-
Saturation of cholesteryl esters produced by human meibomian gland epithelial cells after treatment with rosiglitazone Ocul. Surf. (IF 12.336) Pub Date : 2020-11-26 Jillian F. Ziemanski; Landon Wilson; Stephen Barnes; Kelly K. Nichols
Purpose The purpose of this study was to compare the cholesteryl ester (CE) profiles expressed from human meibomian gland epithelial cells (HMGECs) in response to rosiglitazone-induced differentiation to that of normal human meibum. Methods HMGECs were cultured with rosiglitazone (vehicle control, 20 μM, or 50 μM) and fetal bovine serum (FBS, 2% or 10%) for 2 days or 5 days. Following culture, lipid
-
Single-cell transcriptomics identifies limbal stem cell population and cell types mapping its differentiation trajectory in limbal basal epithelium of human cornea Ocul. Surf. (IF 12.336) Pub Date : 2021-01-01 De-Quan Li; Sangbae Kim; Jin-Miao Li; Qianmiao Gao; Jongsu Choi; Fang Bian; Jiaoyue Hu; Yun Zhang; Jin Li; Rong Lu; Yumei Li; Stephen C. Pflugfelder; Hongyu Miao; Rui Chen
Purpose This study aimed to uncover novel cell types in heterogenous basal limbus of human cornea for identifying LSC at single cell resolution. Methods Single cells of human limbal basal epithelium were isolated from young donor corneas. Single-cell RNA-Sequencing was performed using 10x Genomics platform, followed by clustering cell types through the graph-based visualization method UMAP and unbiased
-
The relationship between dry eye and sleep quality Ocul. Surf. (IF 12.336) Pub Date : 2021-01-06 Morten Schjerven Magno; Tor Paaske Utheim; Harold Snieder; Christopher J. Hammond; Jelle Vehof
Purpose Sleep is an important determinant of health and quality of life. This study aimed to clarify the association between dry eye and sleep quality using a large population-based cohort. Methods 71,761 participants (19–94 yrs, 59.4% female) from the Lifelines cohort in the Netherlands were assessed for dry eye using the Women's Health Study Dry Eye Questionnaire. Sleep quality was evaluated using
-
Aggregated neutrophil extracellular traps occlude Meibomian glands during ocular surface inflammation Ocul. Surf. (IF 12.336) Pub Date : 2021-01-02 Aparna Mahajan; Lenka Hasíková; Ulrike Hampel; Anika Grüneboom; Xiaomei Shan; Irmgard Herrmann; Fabian Garreis; Felix Bock; Jasmin Knopf; Jeeshan Singh; Christine Schauer; Siddharth Mahajan; Moritz Leppkes; Friedrich Paulsen; Ursula Schlötzer-Schrehardt; Veit Krenn; Anselm Jünemann; Bettina Hohberger; Luis E. Muñoz
Purpose Obstructive Meibomian gland dysfunction (MGD) is one of the leading causes of evaporative dry eye disease. Meibomian glands at the eyelid secrete lipids that prevent evaporation of the aqueous tear film. The pathogenesis of obstructive MGD is incompletely understood to date. Herein, we aim to investigate the pathogenesis of obstructive MGD using murine and human samples with various forms of
-
Pigment epithelium-derived factor (PEDF) plays anti-inflammatory roles in the pathogenesis of dry eye disease Ocul. Surf. (IF 12.336) Pub Date : 2021-01-05 Baikai Ma; Yifan Zhou; Rongjun Liu; Kai Zhang; Tingting Yang; Chenxi Hu; Yufei Gao; Qianqian Lan; Yiyun Liu; Xiulan Yang; Hong Qi
Purpose To investigate the expression of pigment epithelium-derived factor (PEDF) in ocular surface in dry eye disease (DED) and its anti-inflammatory roles and mechanisms, clinically and by experiments in vivo and in vitro. Methods A cross-sectional study was conducted to detect the expression of PEDF in tears of dry eye patients by enzyme-linked immunosorbent assay (ELISA). Using dry eye mouse model
-
Sex and age differences in symptoms and signs of dry eye disease in a Norwegian cohort of patients Ocul. Surf. (IF 12.336) Pub Date : 2020-11-24 Sara Tellefsen Nøland; Reza A. Badian; Tor P. Utheim; Øygunn A. Utheim; Aleksandar Stojanovic; Behzod Tashbayev; Sten Raeder; Darlene A. Dartt; Xiangjun Chen
Purpose To investigate sex and age differences in symptoms and signs in a Norwegian clinic-based cohort of patients with dry eye disease (DED). Methods Visitors at the Norwegian Dry Eye Clinic were examined using Ocular Surface Disease Index (OSDI) questionnaire score, tear osmolarity, tear break-up time (TFBUT), ocular surface staining, corneal sensitivity, Schirmer I test, and meibum expressibility
-
Neuroanatomy and neurochemistry of rat cornea: Changes with age Ocul. Surf. (IF 12.336) Pub Date : 2020-12-17 Jiucheng He; Thang Luong Pham; Haydee E.P. Bazan
Purpose To characterize the entire rat corneal nerve architecture, the changes that occur with aging, and its sensory, sympathetic, and parasympathetic fiber distribution. Methods Sprague-Dawley rats (aged 1 day to 2 years old) of both sexes were euthanized, and the whole corneas were immunostained with protein gene product 9.5 (PGP9.5). The specimens were double-labeled with antibodies against calcitonin
-
Handing it to pterygium: Explaining pterygium laterality Ocul. Surf. (IF 12.336) Pub Date : 2020-12-10 Minas T. Coroneo; Lien Tat; Helen Chen; Dimitar I. Grupchev; Christina N. Grupcheva; Matthew H. Ip
Purpose To evaluate any correlation between pterygium laterality and patient handedness. Methods Our study represents a retrospective observational study of a series of consecutive pterygium patients recruited from two centres. Each patient was assessed for their handedness which was compared to the laterality of their presenting pterygium. Patients that possessed bilateral disease comparisons between
-
Creation and grading of experimental corneal scars in mice models Ocul. Surf. (IF 12.336) Pub Date : 2020-11-28 Devon Cogswell; Mei Sun; Erin Greenberg; Curtis E. Margo; Edgar M. Espana
Purpose To develop a stromal wound healing model and a reliable scar classification score system that correlates photographic evaluation with changes in the structure and organization of the extracellular matrix. Materials and methods We tested three stromal injury techniques in adult C57BL/6 mice. Technique 1, a lineal partial thickness keratotomy in the horizontal axis. Technique 2, corneal epithelial
-
Safety and feasibility of mesenchymal stem cell therapy in patients with aqueous deficient dry eye disease Ocul. Surf. (IF 12.336) Pub Date : 2020-11-28 Michael Møller-Hansen; Ann-Cathrine Larsen; Peter Bjerre Toft; Charlotte Duch Lynggaard; Camilla Schwartz; Helle Bruunsgaard; Mandana Haack-Sørensen; Annette Ekblond; Jens Kastrup; Steffen Heegaard
Purpose To evaluate the safety and feasibility of injecting allogeneic adipose-derived mesenchymal stem cells (ASCs) into the lacrimal gland (LG) as a treatment of aqueous deficient dry eye disease (ADDE). Methods In this open-label, 5-visit clinical trial (baseline, treatment and weeks 1, 4 and 16) seven subjects with ADDE received one transconjunctival injection of allogeneic ASCs into the LG in
-
Cicatrizing conjunctivitis as an uncommon manifestation of primary Sjögren's syndrome Ocul. Surf. (IF 12.336) Pub Date : 2020-11-29 Yael Sharon; David Cui; Esen K. Akpek; David S. Chu
Purpose To report occurrence of cicatrizing conjunctivitis as an extraglandular ocular manifestation of primary Sjögren's syndrome (SS). Methods Medical charts of all patients with SS evaluated at two tertiary ophthalmological referral centers were reviewed. Patients who demonstrated clinical findings of cicatrizing conjunctivitis were included in this review. Patient and disease-related data including
-
Relationship between eyelid margin irregularity and meibomian gland dropout Ocul. Surf. (IF 12.336) Pub Date : 2020-11-24 Minji Ha; Jin Soo Kim; Seo-Yeon Hong; Dong-Jin Chang; Woong-Joo Whang; Kyung-Sun Na; Eun Chul Kim; Hyun Seung Kim; Ho Sik Hwang
Purpose To examine the relationship between lid margin abnormalities and meibomian gland loss in infrared meibography. Methods This study was a retrospective chart review of 170 patients with dry eye disease. A correlation analysis between eyelid margin abnormalities and meibomian gland dropout in infrared meibography was performed using data from 141 eyes. We graded the following eyelid margin abnormalities:
-
Ocular surface complications of local anticancer drugs for treatment of ocular tumors Ocul. Surf. (IF 12.336) Pub Date : 2020-11-22 Jayoon Moon; Se Hyun Choi; Min Joung Lee; Dong Hyun Jo; Un Chul Park; Sun-Ok Yoon; Se Joon Woo; Joo Youn Oh
Local chemotherapy is increasingly used, either in combination with surgery or as monotherapy, for management of ocular tumors. Yet many of the local chemotherapeutic agents used for ocular tumors are cytotoxic drugs that are frequently associated with toxicities in normal ocular tissues. Understanding and managing these side effects are important because they affect treatment tolerability, outcome
-
Organoids and organ chips in ophthalmology Ocul. Surf. (IF 12.336) Pub Date : 2020-11-18 Navid Manafi; Fereshteh Shokri; Kevin Achberger; Masatoshi Hirayama; Melika Haji Mohammadi; Farsad Noorizadeh; Jiaxu Hong; Stefan Liebau; Takashi Tsuji; Peter M.J. Quinn; Alireza Mashaghi
-
Assessment of response to multimodal management of neurotrophic corneal disease Ocul. Surf. (IF 12.336) Pub Date : 2020-11-12 Tanya Trinh; Gisella Santaella; Michael Mimouni; Zale Mednick; Eyal Cohen; Nir Sorkin; David S. Rootman; Allan R. Slomovic; Clara C. Chan
Purpose To characterize patients with neurotrophic keratopathy (NK) and describe treatment outcomes. Methods Setting: Two institutional tertiary cornea clinics. Patients: Medical record review of 37 consecutive patients (37 eyes) with NK. Intervention: Management of NK. Main Outcome Measures: Best-corrected visual acuity (BCVA), epithelial defects (ED), re-epithelialization time, number of perforations
-
Prevalence of SARS-CoV-2 in human post-mortem ocular tissues Ocul. Surf. (IF 12.336) Pub Date : 2020-11-08 Onkar B. Sawant; Sneha Singh; Robert Emery Wright; Kayla M. Jones; Michael S. Titus; Eugene Dennis; Eric Hicks; Parag A. Majmudar; Ashok Kumar; Shahzad I. Mian
Background SARS-CoV-2 is found in conjunctival swabs and tears of COVID-19 patients. However, the presence of SARS-CoV-2 has not been detected in the human eye to date. We undertook this study to analyze the prevalence of SARS-CoV-2 in human post-mortem ocular tissues. Methods The expression of SARS-CoV-2 RNA was assessed by RT-PCR in corneal and scleral tissues from 33 surgical-intended donors who
-
Herpes simplex Virus-1 infection in human primary corneal epithelial cells is blocked by a stapled peptide that targets processive DNA synthesis Ocul. Surf. (IF 12.336) Pub Date : 2020-11-06 Hancheng Guan; Manunya Nuth; Vivian Lee; Chenyan Lin; Claire H. Mitchell; Wennan Lu; Richard W. Scott; Michael H. Parker; John L. Kulp; Allen B. Reitz; Robert P. Ricciardi
Purpose Acyclovir is most commonly used for treating ocular Herpes Keratitis, a leading cause of infectious blindness. However, emerging resistance to Acyclovir resulting from mutations in the thymidine kinase gene of Herpes Simplex Virus −1 (HSV-1), has prompted the need for new therapeutics directed against a different viral protein. One novel target is the HSV-1 Processivity Factor which is essential
-
Corneal stromal wound healing: Major regulators and therapeutic targets Ocul. Surf. (IF 12.336) Pub Date : 2020-10-28 Sabeeh Kamil; Rajiv R. Mohan
Corneal stromal wound healing is a complex event that occurs to restore the transparency of an injured cornea. It involves immediate apoptosis of keratocytes followed by their activation, proliferation, migration, and trans-differentiation to myofibroblasts. Myofibroblasts contract to close the wound and secrete extracellular matrix and proteinases to remodel it. Released proteinases may degenerate
-
Role of transient receptor potential melastatin 8 activity in menthol-induced cold sensitivity and its qualitative perception in dry eye Ocul. Surf. (IF 12.336) Pub Date : 2020-10-27 Minako Kaido; Sachiko Inoue; Motoko Kawashima; Reiko Ishida; Shigeru Nakamura; Kazuo Tsubota
Purpose To evaluate the role of transient receptor potential melastatin 8 (TRPM8) activity in menthol-induced cold sensitivity and its qualitative perception in patients with dry eye (DE). Methods This prospective, cross-sectional, comparative study included 52 eyes of 52 subjects (mean age: 66.8 ± 9.2 years; range: 44–86) with a tear break-up time (TBUT) of ≤5 s. The participants were classified into
-
Management of corneal complications in vernal keratoconjunctivitis: A review Ocul. Surf. (IF 12.336) Pub Date : 2020-10-24 Sepehr Feizi; Mohammad Ali Javadi; Mohammadhasan Alemzadeh-Ansari; Amir Arabi; Toktam Shahraki; Ahmad Kheirkhah
Vernal keratoconjunctivitis (VKC) is a chronic, bilateral, allergic conjunctivitis with episodes of acute exacerbations. Although VKC has a self-limiting course, chronic recurrent inflammation can cause long-term visual impairment due to corneal complications including shield ulcers, infectious keratitis, keratoconus, corneal opacities, and limbal stem cell deficiency. The initial step in the management
-
Conjunctival transcriptome analysis reveals the overexpression of multiple pattern recognition receptors in vernal keratoconjunctivitis Ocul. Surf. (IF 12.336) Pub Date : 2020-10-21 Andrea Leonardi; Philippe Daull; Jean-Sébastien Garrigue; Fabiano Cavarzeran; Mylène Docquier; Antonino Di Stefano; Elena Tarricone; Brun Paola
-
Matrix metalloproteinase 9 positivity predicts long term decreased tear production Ocul. Surf. (IF 12.336) Pub Date : 2020-10-21 Matias Soifer; Hazem M. Mousa; Sandra S. Stinnett; Anat Galor; Victor L. Perez
Purpose To investigate long-term correlations between Matrix Metalloproteinase-9 (MMP-9) testing and dry eye (DE) parameters. Additionally, to evaluate variability in MMP-9 results over time and with anti-inflammatory treatment. Methods Retrospective cohort study of DE patients with equal MMP-9 testing results (positive or negative) in both eyes and a minimum of 6 months of follow up. Our main outcome
-
Pain sensitivity and autonomic nervous system parameters as predictors of dry eye symptoms after LASIK Ocul. Surf. (IF 12.336) Pub Date : 2020-10-21 Alexandra E. Levitt; Anat Galor; Leslie Small; William Feuer; Elizabeth R. Felix
Purpose Differences in pain processing and autonomic function among patients have been implicated the development of chronic pain after surgery. This study was designed to evaluate whether pain and autonomic metrics predict severity of chronic dry eye (DE) symptoms after LASIK, as there is increasing evidence that DE symptoms may be manifestations of persistent post-operative ocular pain. Methods Secondary
-
A novel transillumination meibography device for in vivo imaging of mouse meibomian glands Ocul. Surf. (IF 12.336) Pub Date : 2020-10-16 Ho Sik Hwang; Eric Mikula; Yilu Xie; Donald J. Brown; James V. Jester
Purpose While mouse models of dry eye disease (DED) have been developed, studies evaluating the role of the meibomian glands limited by the inability to temporally document changes. In this report we describe the development of a novel mouse transillumination meibography device and assess the ability of this device to detect age-related changes in the meibomian glands of young and old mice. Methods
-
Disruption of blood-aqueous barrier in dry eye disease Ocul. Surf. (IF 12.336) Pub Date : 2020-10-13 Hossein Aghaei; Ahmad Kheirkhah; Acieh Es' haghi; Mohammad Reza Aghamirsalim; Soheila Asgari; Mohammad Mirzakhan kordamiri
Purpose To evaluate level of flare in aqueous humor of dry eye disease (DED) and compare it with normal controls. Methods In this cross-sectional study, we compared the anterior chamber flare between 28 patients with DED (the DED group) and 27 normal age- and gender-matched controls (the control group). DED group was divided in Sjӧgren's syndrome dry eye (SDE, n = 10) and non- Sjӧgren's syndrome dry
-
Anterior-segment spectral domain optical coherence tomography in epidermolysis bullosa Ocul. Surf. (IF 12.336) Pub Date : 2020-09-22 Vicki M. Chen; Nihaal Mehta; Calvin C. Robbins; Elizabeth Noh; Varsha Pramil; Jay S. Duker; Nadia K. Waheed
Purpose Assess epidermolysis bullosa (EB)-related corneal pathology using anterior segment optical coherence tomography (AS-OCT) and correlate imaging with clinical metrics in EB patients versus age-matched controls. Methods EB patients and controls were recruited during an EB conference (July 2018) and at Tufts Medical Center (June–August 2019). Subjects completed a questionnaire, had best corrected
-
Comparing the prophylactic action of lipid and non-lipid containing tear supplements in adverse environmental conditions: A randomised crossover trial. Ocul. Surf. (IF 12.336) Pub Date : 2020-08-15 Alex Muntz,Sanjay Marasini,Michael T M Wang,Jennifer P Craig
Purpose To evaluate the prophylactic benefits of lipid-based and non-lipid-based artificial tear lubricants, in dry eye disease, after adverse environmental exposure. Methods Twenty-eight participants with dry eye disease were recruited in a prospective, double-masked, randomised crossover trial. On separate days, participants were randomised to receive a single application of a lipid-containing tear
-
Use of digital displays and ocular surface alterations: A review Ocul. Surf. (IF 12.336) Pub Date : 2020-10-11 Cristian Talens-Estarelles; Alejandro Cervino; José Vicente García-Marqués; Santiago García-Lázaro
Digital display use has been accepted to be implicated as a contributing factor for dry eye disease (DED). Abnormal blinking during computer operation, including a reduced blink rate and an incomplete eyelid closure, increased palpebral fissure as consequence of high visualization angles, and meibomian gland dysfunction associated to long-term display use, are behind the increased prevalence of dry
-
The molecular basis of neurotrophic keratopathy: Diagnostic and therapeutic implications. A review Ocul. Surf. (IF 12.336) Pub Date : 2020-10-03 Ruiz-Lozano Raul E; Hernandez-Camarena Julio C; D. Loya-Garcia; Merayo-Lloves Jesus; Rodriguez-Garcia Alejandro
Neurotrophic keratopathy (NK) is a degenerative corneal disease produced by different factors, including infection, trauma, and neurogenesis, that lead to trigeminal nerve damage and impaired corneal sensitivity. Extensive epithelial breakdown, impaired corneal epithelial healing and corneal ulceration, stromal melting, and perforation are main NK features. The proliferation of the corneal epithelium
-
Profile of tear lipid mediator as a biomarker of inflammation for meibomian gland dysfunction and ocular surface diseases: Standard operating procedures Ocul. Surf. (IF 12.336) Pub Date : 2020-10-03 Yohannes Abere Ambaw; Dhayan Parresh Timbadia; Manfred Raida; Federico Torta; Markus R. Wenk; Louis Tong
Human tear is a biological fluid rich in lipids that is increasingly collected in clinical and biological research. The repertoire of small lipids or lipid mediators (often termed eicosanoids or oxylipins) found in human tear provides insight into metabolism of fatty acids and physiology of the ocular surface and Meibomian glands. Disturbances in the tear lipid mediators profile also occur during inflammation
-
Decellularized porcine conjunctiva as an alternative substrate for tissue-engineered epithelialized conjunctiva. Ocul. Surf. (IF 12.336) Pub Date : 2020-08-27 Joana Witt,Jana Dietrich,Sonja Mertsch,Stefan Schrader,Kristina Spaniol,Gerd Geerling
Purpose The long-term success of visual rehabilitation in patients with severe conjunctival scarring is reliant on the reconstruction of the conjunctiva with a suitable substitute. The purpose of this study is the development and investigation of a re-epithelialized conjunctival substitute based on porcine decellularized conjunctiva (PDC). Methods PDC was re-epithelialized either with pre-expanded
-
Ocular surface diabetic disease: A neurogenic condition? Ocul. Surf. (IF 12.336) Pub Date : 2020-10-01 Antonio Di Zazzo; Marco Coassin; Alessandra Micera; Tommaso Mori; Maria De Piano; Luca Scartozzi; Roberto Sgrulletta; Stefano Bonini
Purpose To investigate clinical and inflammatory changes in the ocular surface of insulin-dependent type I diabetic patients. Methods Two hundred and nine eyes of 106 patients with diagnosis of type I diabetes were recruited in a prospective observational study. Ocular surface clinical assessment, corneal sensitivity and tear film stability tests were performed to evaluate ocular surface system function
-
Retroprosthetic membrane: A complication of keratoprosthesis with broad consequences. Ocul. Surf. (IF 12.336) Pub Date : 2020-09-09 Jennifer Park,Pakornkit Phrueksaudomchai,M Soledad Cortina
Keratoprosthesis (KPro) devices have the remarkable ability to restore vision in patients suffering from corneal blindness who are poor candidates for traditional penetrating keratoplasty. However, eyes with KPro can experience various complications, including the development of retroprosthetic membrane (RPM). RPMs reduce visual acuity in patients due to physical obstruction of the visual axis, but
-
Latest evidences on meibomian gland dysfunction diagnosis and management. Ocul. Surf. (IF 12.336) Pub Date : 2020-09-12 Edoardo Villani,Luca Marelli,Andrea Dellavalle,Massimiliano Serafino,Paolo Nucci
Meibomian gland dysfunction (MGD) can be considered the leading cause of dry eye disease (DED) and one of the most common ophthalmic disorders found in clinical practice. The growing body of literature provides a substantial amount of information on this condition, but more efforts are needed to better interpret research data and to properly apply them to daily clinical practice., In this article,
-
Ocular surface microbiota in patients with aqueous tear-deficient dry eye. Ocul. Surf. (IF 12.336) Pub Date : 2020-09-12 Jasmine Andersson,Josef K Vogt,Marlene D Dalgaard,Oluf Pedersen,Kim Holmgaard,Steffen Heegaard
Purpose An altered ocular surface microbiota may contribute to the pathophysiology of dry eye disease. The aim of the study was to explore potential differences in microbiota diversity and composition in aqueous tear-deficient dry eye (with and without ocular graft-versus-host disease) compared with controls. Methods Swab samples from the inferior fornix of the conjunctiva were obtained from patients
-
2D fourier transform for global analysis and classification of meibomian gland images. Ocul. Surf. (IF 12.336) Pub Date : 2020-09-08 K Ciężar,M Pochylski
In recent years, significant progress has been made in the Meibography technique resulting from the use of advanced image analysis methods allowing a quantitative description of the Meibomian gland structures. Many objective measures of gland distortion were previously proposed allowing for user-independent classification of acquired gland images. However, due to the complicated nature of gland deformation
-
Activation of ocular surface mast cells promotes corneal neovascularization. Ocul. Surf. (IF 12.336) Pub Date : 2020-09-08 WonKyung Cho,Sharad K Mittal,Elsayed Elbasiony,Sunil K Chauhan
Purpose Mast cells, historically known for their effector function in the induction of allergic diseases, reside in all vascularized tissues of the body in particular proximity to blood and lymphatic vessels. As neighboring sentinel cells to blood vessels, mast cells have been associated with angiogenesis. Here we assess the direct contribution of mast cells to neovascularization at the ocular surface
-
Enhanced closed eye neutrophil degranulation in dry eye disease. Ocul. Surf. (IF 12.336) Pub Date : 2020-09-02 Cameron K Postnikoff,Katherine Held,Veena Viswanath,Kelly K Nichols
-
A randomized, sham-controlled trial of intraductal meibomian gland probing with or without topical antibiotic/steroid for obstructive meibomian gland dysfunction. Ocul. Surf. (IF 12.336) Pub Date : 2020-08-31 Ahmad Kheirkhah,Hidenaga Kobashi,Jessica Girgis,Arsia Jamali,Joseph B Ciolino,Pedram Hamrah
Importance Obstructive meibomian gland dysfunction (MGD) can be refractory to medical therapy. Intraductal meibomian gland (MG) probing may offer a potential therapeutic approach for these patients, but no randomized trials have been conducted to date. Objective To assess clinical changes after intraductal MG probing for patients with refractory obstructive meibomian gland dysfunction. Design Randomized
-
Efficacy and tolerability of nortriptyline in the management of neuropathic corneal pain. Ocul. Surf. (IF 12.336) Pub Date : 2020-08-27 M Cuneyt Ozmen,Gabriela Dieckmann,Stephanie M Cox,Ramy Rashad,Rumzah Paracha,Nedda Sanayei,Melina I Morkin,Pedram Hamrah
Purpose Neuropathic corneal pain (NCP) is a recently acknowledged disease entity. However, there is no consensus in potential treatment strategies, particularly in patients with a centralized component of pain. This study aims to assess the efficacy and tolerability of the tricyclic antidepressant, nortriptyline, among NCP patients. Methods Patients with clinically diagnosed NCP and a centralized component
-
Association of meibomian gland morphology with symptoms and signs of dry eye disease in the Dry Eye Assessment and Management (DREAM) study. Ocul. Surf. (IF 12.336) Pub Date : 2020-08-25 Ebenezer Daniel,Maxwell Pistilli,Gui-Shuang Ying,Vatinee Y Bunya,Mina Massaro-Giordano,Penny A Asbell,Maureen G Maguire,
Purpose To describe associations between symptoms and signs of dry eye disease (DED) and meibomian gland (MG) morphology. Methods Cross-sectional study utilizing data from the Dry Eye Assessment and Management (DREAM) Study. Readers graded MG features in the middle third of upper and lower lids on infrared meibography images. Associations with signs and symptoms of DED were evaluated with adjustment
-
Decellularized porcine cornea-derived hydrogels for the regeneration of epithelium and stroma in focal corneal defects. Ocul. Surf. (IF 12.336) Pub Date : 2020-08-22 Fuyan Wang,Weiyun Shi,Hua Li,Hongwei Wang,Dapeng Sun,Long Zhao,Lingling Yang,Ting Liu,Qingjun Zhou,Lixin Xie
Purpose Hydrogels derived from decellularized tissues provide superior biocompatibility, tenability and tissue-specific extracellular matrix (ECM) components. Based on the preparation of decellularized porcine cornea (DPC), here we developed an injectable and transparent hydrogel for the regeneration of epithelium and stroma in focal corneal defects. Methods The DPC-derived hydrogel was prepared with
-
Role of diurnal variation of corneal sensory processing in contact lens discomfort. Ocul. Surf. (IF 12.336) Pub Date : 2020-08-20 Ping Situ,Trefford L Simpson,Carolyn G Begley,Nancy Keir
Purpose To examine the diurnal variation of corneal threshold and suprathreshold sensory processing, symptoms, and tear secretion in symptomatic and asymptomatic contact lens (CL) wearers and controls. Methods 26 symptomatic and 25 asymptomatic CL wearers and 15 asymptomatic non-CL wearing controls participated. Cooling thresholds, symptoms and tear meniscus height (TMH) were measured on each of 3
-
Efficacy of cyanoacrylate tissue adhesive in the management of corneal thinning and perforation due to microbial keratitis. Ocul. Surf. (IF 12.336) Pub Date : 2020-08-19 Rohan Bir Singh,Shuyan Zhu,Ann Yung,Thomas H Dohlman,Reza Dana,Jia Yin
Purpose Report the efficacy of cyanoacrylate tissue adhesive (CTA) application in the management of corneal thinning and perforations associated with microbial keratitis. Methods A retrospective review of consecutive patients who underwent CTA application for corneal thinning and perforation secondary to microbiologically proven infectious keratitis between 2001 and 2018 at a single center. We defined
-
Dry eye disease in children and adolescents in India. Ocul. Surf. (IF 12.336) Pub Date : 2020-08-18 Pragnya R Donthineni,Anthony Vipin Das,Sayan Basu
Purpose To describe the etiological causes and clinical profile of dry eye disease (DED) among children and adolescents seeking eye care in India. Methods This was a cross-sectional study of 1023 new patients (≤21 years of age) diagnosed with DED presenting between 2010 and 2018 to a multi-tier ophthalmology hospital network. Children and adolescents with both symptoms and signs of dry eyes were considered
-
Migratory serpiginous corneal epitheliopathy (MSCE)- details of 4 cases. Ocul. Surf. (IF 12.336) Pub Date : 2020-08-18 Amrita Mohanty,Ruchi Mittal,Ashi Khurana,Sanjay Chanda,Smrutirekha Priyadarshini,Srikant K Sahu
Background and objective To describe four cases of recurrent, fleeting corneal epithelial lesion, migratory serpiginous corneal epitheliopathy (MSCE). Method Description of cases, histopathology and discussion of treatment outcome. Results The study included 4 consecutive male patients; mean age was 21.25 years. Only one eye was affected. The common symptoms were irritation and tearing. Typical interpalpebral
-
Inflammatory status predicts contact lens discomfort under adverse environmental conditions. Ocul. Surf. (IF 12.336) Pub Date : 2020-08-16 Itziar Fernández,Alberto López-Miguel,Vicente Martín-Montañez,Amalia Enríquez-de-Salamanca,Margarita Calonge,José M González-Méijome,María J González-García
Purpose To characterize and predict the clinical and tear molecular response of contact lens (CL) wearers exposed to a controlled adverse desiccating environment (CADE). Methods Objective and subjective variables and tear cytokine levels were evaluated of monthly silicone hydrogel CL wearers pre- and post-90 min of CADE exposure. Unsupervised hierarchical agglomerative clustering based on relative
-
The impact of euthanasia and enucleation on mouse corneal epithelial axon density and nerve terminal morphology. Ocul. Surf. (IF 12.336) Pub Date : 2020-08-13 Gauri Tadvalkar,Sonali Pal-Ghosh,Ahdeah Pajoohesh-Ganji,Mary Ann Stepp
Introduction Here we study the impact of using either CO2 gas or cervical dislocation (CD) for euthanasia and using different techniques to enucleate the eye on preserving axonal density and morphology of the intraepithelial corneal nerves (ICNs). Objectives To determine whether using CO2 gas or CD for euthanasia and enucleating by cutting or pulling eyes out impacts axon density and nerve terminal
-
Characterising the ocular surface and tear film in a population-based birth cohort of 45-year old New Zealand men and women. Ocul. Surf. (IF 12.336) Pub Date : 2020-08-13 Jennifer P Craig,Michael T M Wang,Antony Ambler,Kirsten Cheyne,Graham A Wilson
Purpose To assess the prevalence of dry eye disease, aqueous tear deficiency, meibomian gland dysfunction, and asymptomatic ocular surface disease in a population-based cohort of 45-year-old New Zealand men and women. Methods This cross-sectional study of 885 participants (442 females, 443 males) was based on a population-representative birth cohort of individuals born between April 1 1972 and March
-
FDA review times for new drugs in ophthalmology. Ocul. Surf. (IF 12.336) Pub Date : 2020-08-11 Michelle A Carpenter,Gary D Novack
-
Association of dry eye disease and sun exposure in geographically diverse adult (≥40 years) populations of India: The SEED (sun exposure, environment and dry eye disease) study - Second report of the ICMR-EYE SEE study group. Ocul. Surf. (IF 12.336) Pub Date : 2020-08-09 Radhika Tandon,Praveen Vashist,Noopur Gupta,Vivek Gupta,Pranita Sahay,Dipali Deka,Sachchidanand Singh,K Vishwanath,G V S Murthy
Purpose To estimate the prevalence and determine risk factors for dry eye disease (DED) in geographically diverse regions of India. Method A population based cross-sectional study was conducted on people aged ≥40 years in plain, hilly and coastal areas. Dry eye assessment by objective [tear film break-up time (TBUT), Schirmer I, corneal staining] and subjective [Ocular Surface Disease Index (OSDI)]
-
Management of corneal melt in patients with Boston Keratoprosthesis Type 1: Repair versus repeat. Ocul. Surf. (IF 12.336) Pub Date : 2020-08-07 Roy Daoud,Saama Sabeti,Mona Harissi-Dagher
Purpose To evaluate outcomes of Boston keratoprosthesis (KPro) repair versus repeat KPro as treatment for corneal melt in KPro patients. Methods Retrospective study of adult KPro patients with melt managed by KPro repair or repeat KPro by one surgeon (MH-D). Incidence of sight- or globe-threatening complications, risk of recurrence of melt and change in BCVA before and 3 months after the procedure
-
Ageing and the natural history of dry eye disease: A prospective registry-based cross-sectional study. Ocul. Surf. (IF 12.336) Pub Date : 2020-08-03 Michael T M Wang,Alex Muntz,Joevy Lim,Ji Soo Kim,Lucas Lacerda,Aditya Arora,Jennifer P Craig
Purpose To investigate the impact of ageing on ocular surface parameters, and empirically determine optimal prognostic cut-off ages for clinical markers of dry eye disease, aqueous tear deficiency, and meibomian gland dysfunction. Methods A total of 1331 community residents (785 females, 546 males; mean ± SD age, 38 ± 19 years) were recruited in a prospective registry-based cross-sectional study. Dry
-
Efficacy and retention of silicone punctal plugs for treatment of dry eye in patients with and without ocular graft-versus-host-disease. Ocul. Surf. (IF 12.336) Pub Date : 2020-07-30 Rohan Bir Singh,Ann Yung,Giulia Coco,Shruti Sinha,Thomas H Dohlman,Jia Yin,Reza Dana
Purpose To examine the retention rates and efficacy of silicone punctal plugs for the treatment of dry eye disease (DED) in patients with ocular graft-versus-host-disease (oGVHD) in comparison to dry eye disease due to non-oGVHD etiologies. Methods We reviewed the case-records of 864 consecutive patients with DED who were symptomatic despite topical therapy and had silicone punctal plugs placed over
-
A reliable animal model of corneal stromal opacity: Development and validation using in vivo imaging. Ocul. Surf. (IF 12.336) Pub Date : 2020-07-29 Vineet Joshi,Shiva Vaishnavi K,Sanjay Kumar Ojha,Vivek Singh,Sayan Basu
Purpose To validate an animal model of corneal stromal opacity by using objective vision-independent in vivo imaging metrics. Methods This was a prospective study, with two arms: (i) observational human arm which included 14 patients with healed unilateral ulcerative keratitis; and (ii) experimental rabbit arm, which included 6 New Zealand white rabbits. A 3-mm central wound was created in the left
-
NLRP12- and NLRC4-mediated corneal epithelial pyroptosis is driven by GSDMD cleavage accompanied by IL-33 processing in dry eye. Ocul. Surf. (IF 12.336) Pub Date : 2020-07-29 Hui Chen,Xiaoliang Gan,Yonghao Li,Jianjun Gu,Yizhi Liu,Yang Deng,Xiaoran Wang,Yanhua Hong,Yixin Hu,Lishi Su,Wei Chi
Purpose Dry eye disease (DED) is a common and multifactor-induced autoimmune ocular surface disease. Environmental factors, such as desiccating stress (DS) and hyperosmolarity, affect the corneal epithelium to induce ocular surface inflammation in DED. We aimed to explore the potential mechanisms by which innate immunity and pyroptosis are initiated in the mucosal epithelium in response to environmental
-
New insights into the lacrimal pump. Ocul. Surf. (IF 12.336) Pub Date : 2020-07-28 Mohammad Javed Ali,Maximilian Zetzsche,Michael Scholz,Dieter Hahn,Simone Gaffling,Jens Heichel,Christian Manfred Hammer,Lars Bräuer,Friedrich Paulsen
Purpose To date, there are many theories about tear transport through the canaliculi of the draining lacrimal system into the lacrimal sac but only few with supportive data. It is certain that the function of the lacrimal part of orbicularis oculi muscle (Horner-Duverney's muscle) is indispensable for the transport of “used” tears. However, the muscle's exact structure and the mechanisms of its functions