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Rapid identification of SARS‐CoV‐2 variants using stable high‐frequency mutation sites APMIS (IF 2.8) Pub Date : 2024-03-16 Yu Fu, Xiaobai He, Quan Fang, Fei Kong, Yan Zhang, Ting Fu, Liang Chen, YanXin Liu, Zhen Wang, Jianxin Lyu, Linjie Chen
Respiratory infectious viruses, including SARS‐CoV‐2, undergo rapid genetic evolution, resulting in diverse subtypes with complex mutations. Detecting and differentiating these subtypes pose significant challenges in respiratory virus surveillance. To address these challenges, we integrated ARMS‐PCR with molecular beacon probes, allowing selective amplification and discrimination of subtypes based
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Multiplex PCR for respiratory bacteria in acute care APMIS (IF 2.8) Pub Date : 2024-03-14 Elina Saarela, Marjo Renko, Matti Uhari, Tytti Pokka, Heikki Kauma, Terhi S. Ruuska
The purpose of the study was to evaluate the clinical utility of multiplex PCR for detecting bacterial respiratory pathogens in nasopharyngeal samples. Acutely ill adults in the emergency department with respiratory infection symptoms, fever, chest pain or poor general condition were enrolled for this cohort study. Samples were stored at –70 °C until being analysed with multiplex PCR for seven respiratory
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Exploring the significance of microbiota metabolites in rheumatoid arthritis: uncovering their contribution from disease development to biomarker potential APMIS (IF 2.8) Pub Date : 2024-03-12 Zi‐feng Lu, Chou‐Yi Hsu, Nada Khairi Younis, Mohammed Ahmed Mustafa, Elena A. Matveeva, Yassien Hussain Owaied Al‐Juboory, Mohaned Adil, Zainab H. Athab, Mustafa Nasrat Abdulraheem
Rheumatoid arthritis (RA) is a multifaceted autoimmune disorder characterized by chronic inflammation and joint destruction. Recent research has elucidated the intricate interplay between gut microbiota and RA pathogenesis, underscoring the role of microbiota‐derived metabolites as pivotal contributors to disease development and progression. The human gut microbiota, comprising a vast array of microorganisms
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The autonomic nerves around the vein of Marshall: a postmortem study with clinical implications APMIS (IF 2.8) Pub Date : 2024-03-12 Denis Depes, Ari Mennander, Paavo Immonen, Artturi Mäkinen, Heini Huhtala, Timo Paavonen, Ivana Kholová
This study aims to analyze the vein of Marshall (VOM) in human autopsy hearts and its correlation with clinical data to elucidate the morphological substrates of atrial fibrillation (AF) and other cardiac diseases. Twenty‐three adult autopsy hearts were studied, assessing autonomic nerves by immunohistochemistry with tyrosine hydroxylase (sympathetic nerves), choline acetyltransferase (parasympathetic
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Antifungal drug resistance in Candida: a special emphasis on amphotericin B APMIS (IF 2.8) Pub Date : 2024-03-11 Lailema Ahmady, Manisha Gothwal, Muhammed Mushthaque Mukkoli, Vinay Kumar Bari
Invasive fungal infections in humans caused by several Candida species, increased considerably in immunocompromised or critically ill patients, resulting in substantial morbidity and mortality. Candida albicans is the most prevalent species, although the frequency of these organisms varies greatly according to geographic region. Infections with C. albicans and non‐albicans Candida species have become
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Surgery of enlarging lesions after stereotactic radiosurgery for brain metastases in patients with non‐small cell lung cancer with oncogenic driver mutations frequently reveals radiation necrosis: case series and review APMIS (IF 2.8) Pub Date : 2024-03-11 Fang Zhou, Leilei Jiang, Xuankai Sun, Zhen Wang, Jialin Feng, Ming Liu, Zhao Ma
In brain metastases, radiation necrosis (RN) is a complication that arises after single or multiple fractionated stereotactic radiosurgery (SRS/FSRS), which is challenging to distinguish from local recurrence (LR). Studies have shown increased RN incidence rates in non‐small cell lung cancer (NSCLC) patients with oncogenic driver mutations (ODMs) or receiving tyrosine kinase inhibitors (TKIs). This
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Understanding the biofilm development of Acinetobacter baumannii and novel strategies to combat infection APMIS (IF 2.8) Pub Date : 2024-03-06 Naji Naseef Pathoor, Akshaya Viswanathan, Gulshan Wadhwa, Pitchaipillai Sankar Ganesh
Acinetobacter baumannii (A. baumannii) is a Gram‐negative, nonmotile, and aerobic bacillus emerged as a superbug, due to increasing the possibility of infection and accelerating rates of antimicrobial agents. It is recognized as a nosocomial pathogen due to its ability to form biofilms. These biofilms serve as a defensive barrier, increase antibiotic resistance, and make treatment more difficult. As
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Pathological image profiling identifies onco‐microbial, tumor immune microenvironment, and prognostic subtypes of colorectal cancer APMIS (IF 2.8) Pub Date : 2024-02-26 Yuwen Chu, Shuo Zhang, Wei Wan, Jialiang Yang, Yumeng Zhang, Chuanqi Nie, Weipeng Xing, Shanhe Tong, Jinyang Liu, Geng Tian, Bing Wang, Lei Ji
Histology slide, tissue microbes, and the host gene expression can be independent prognostic factors of colorectal cancer (CRC), but the underlying associations and biological significance of these multimodal omics remain unknown. Here, we comprehensively profiled the matched pathological images, intratumoral microbes, and host gene expression characteristics in 527 patients with CRC. By clustering
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Informed development of a multi‐species biofilm in chronic obstructive pulmonary disease APMIS (IF 2.8) Pub Date : 2024-02-21 Bryn Short, Christopher Delaney, William Johnston, Gary J. Litherland, John C. Lockhart, Craig Williams, William G. Mackay, Gordon Ramage
Recent evidence indicates that microbial biofilm aggregates inhabit the lungs of COPD patients and actively contribute towards chronic colonization and repeat infections. However, there are no contextually relevant complex biofilm models for COPD research. In this study, a meta‐analysis of the lung microbiome in COPD was used to inform development of an optimized biofilm model composed of genera highly
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Humoral immune response of Galleria mellonella after mono- and co-injection with Hypericum perforatum extract and Candida albicans APMIS (IF 2.8) Pub Date : 2024-02-12 Tülay Turgut Genç, Serhat Kaya, Melih Günay, Çağla Çakaloğlu
Galleria mellonella is used as a model organism to study the innate immune response of insects. In this study, the humoral immune response was assessed by examining phenoloxidase activity, fungal burden, and the expression of phenoloxidase and antimicrobial peptide genes at different time point following separate and combined injections of Hypericum perforatum extract and a nonlethal dose of Candida
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Inflammatory mediators in bacterial vaginosis: The role of cytokines APMIS (IF 2.8) Pub Date : 2024-02-12 Yuexin Zhang, Zhi He
BV is a significant concern in women's health with a varying prevalence rate in different cities of China. The condition has been linked to the acquisition of STIs, including HIV and HPV, and can lead to infertility, adverse obstetric outcomes. We conducted a comprehensive literature search in the PubMed. The search was performed from 01/01/2018 to 01/09/2023. The following search terms were used:
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Bacterial aerobic respiration is a major consumer of oxygen in sputum from patients with acute lower respiratory tract infection APMIS (IF 2.8) Pub Date : 2024-01-29 Peter Østrup Jensen, Pernille Olsen, Arnold Matovu Dungu, Gertrud Baunbæk Egelund, Andreas Vestergaard Jensen, Pernille Ravn, Birgitte Lindegaard, Frederik Boëtius Hertz, Thomas Bjarnsholt, Daniel Faurholt-Jepsen, Mette Kolpen
Bacterial aerobic respiration may determine the outcome of antibiotic treatment in experimental settings, but the clinical relevance of bacterial aerobic respiration for the outcome of antibiotic treatment has not been tested. Therefore, we hypothesized that bacterial aerobic respiration is higher in sputum from patients with acute lower respiratory tract infections (aLRTI), than in sputum from patients
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Virology and safety profile of Molnupiravir at three different doses for treatment of SARS-CoV-2: a systematic review and meta-analysis APMIS (IF 2.8) Pub Date : 2024-01-30 Mahnoor Sukaina, Syed Hasan Shuja, Syeda Tayyaba Rehan, Sidhant Ochani, Muhammad Sheryar
Molnupiravir is incorporated into the viral genome, thereby increasing errors, mismatching, and misdirecting the viral polymerase thereby, halting viral RNA replication of SARS-CoV-2. Following PRISMA guidelines, a thorough literature search was performed on electronic and medical databases from December 2022 till January 2023. Molnupiravir 800 mg showed significance in creating viral RNA error rate
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Clinical implications of AGR2 in primary prostate cancer: Results from a large-scale study APMIS (IF 2.8) Pub Date : 2024-01-30 Moritz Wambach, Matteo Montani, Josefine Runz, Carsten Stephan, Klaus Jung, Holger Moch, Daniel Eberli, Marit Bernhardt, Oliver Hommerding, Tobias Kreft, Marcus V. Cronauer, Anika Kremer, Thomas Mayr, Stefan Hauser, Glen Kristiansen
Human anterior gradient-2 (AGR2) has been implicated in carcinogenesis of various solid tumours, but the expression data in prostate cancer are contradictory regarding its prognostic value. The objective of this study is to evaluate the expression of AGR2 in a large prostate cancer cohort and to correlate it with clinicopathological data. AGR2 protein expression was analysed immunohistochemically in
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Immune responses to mRNA-based vaccines given as a third COVID-19 vaccine dose in people living with HIV—a literature review APMIS (IF 2.8) Pub Date : 2024-01-26 Maria Lodberg Knudsen, Susanne Dam Nielsen, Line Dam Heftdal
People living with HIV (PLWH) were not included in the first efficacy studies of mRNA vaccines against SARS-CoV-2. In this literature review, we investigate evidence of humoral and cellular immunity after a third dose of an mRNA vaccine in PLWH. We performed a literature search in PubMed, Embase, Web of Science and SCOPUS published between 1 January 2020 and 31 December 2022. Selection criteria were
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Novel sampling technique maintaining the two-dimensional organization of microbes during cultivation from chronic wounds: The Imprint method APMIS (IF 2.8) Pub Date : 2024-01-25 Anne Kristine Servais Iversen, Blaine Gabriel Fritz, Mads Joachim Hansen, Klaus Kirketerp-Møller, Tim Holm Jakobsen, Thomas Bjarnsholt, Mads Lichtenberg
This study aimed to develop and validate “the Imprint method,”, a technique for sampling microbes from chronic wounds while preserving their two-dimensional spatial organization. We used nylon filters to sample bacteria and compared with sampling using Eswabs in 12 patients. The Imprint method identified a mean of 0.93 unique species more than Eswab (4.3 ± 2.2 and 3.4 ± 1.4 unique species, respectively;
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Close monitoring and early intervention: management principles for cystic fibrosis in Denmark APMIS (IF 2.8) Pub Date : 2024-01-24 Tavs Qvist, Bibi Uhre Nielsen, Hanne Vebert Olesen, Inger Hee Mabuza Mathiesen, Daniel Faurholt-Jepsen, Terese L. Katzenstein, Jannik Helweg-Larsen, Frederikke Rönsholt, Majbritt Jeppesen, Mette Frahm Olsen, Frederik Fouirnaies Buchvald, Kim Gjerum Nielsen, Søren Jensen-Fangel, Tania Pressler, Marianne Skov
Cystic fibrosis (CF) care in Denmark has been characterized by close monitoring and pre-emptive treatment of lung disease and other CF-related complications. Continuous evaluation through data collection and commitment to clinical research has incrementally improved outcomes. This approach has been in line with best practices set forth by European Standards of Care but has also gone beyond Society
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Metabolic drives affecting Th17/Treg gene expression changes and differentiation: impact on immune-microenvironment regulation APMIS (IF 2.8) Pub Date : 2024-01-18 Carolina Brescia, Salvatore Audia, Alessia Pugliano, Federica Scaglione, Rodolfo Iuliano, Francesco Trapasso, Nicola Perrotti, Emanuela Chiarella, Rosario Amato
The CD4+ T-cell population plays a vital role in the adaptive immune system by coordinating the immune response against different pathogens. A significant transformation occurs in CD4+ cells during an immune response, as they shift from a dormant state to an active state. This transformation leads to extensive proliferation, differentiation, and cytokine production, which contribute to regulating and
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Functional studies with IgM and IgA immunoglobulins: binding to pIgR, FcαμR, FcμR, and CDC activities APMIS (IF 2.8) Pub Date : 2024-01-17 Hanna Beyer, Mark Sommerfeld, Kaj Grandien, Christine Faust, Bodo Tillmann, Wulf Dirk Leuschner, Anne Régnier-Vigouroux, Sandra Weil, Ercole Rao, Thomas Langer
IgMs are the first antibodies produced by the immune system upon encounter of a possible pathogen and are one of five antibody subclasses in humans. For IgG, the most intensively studied antibody class, the N-linked glycosylation site located in the Fc-domain is directly involved in high affinity binding to the respective receptors and initiation of corresponding immune response. IgM molecules have
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FilmArray (BCID2) provides essential and timely results in bloodstream infections in small acute care hospitals without conventional microbiology services APMIS (IF 2.8) Pub Date : 2024-01-12 Hege Harboe-Sjåvik, Kristoffer Hammer Endresen, Sandra Åsheim, Arnfinn Sundsfjord
We have evaluated the performance of FilmArray BCID2 in reactive blood cultures in a small acute care hospital compared to conventional diagnostics at a regional microbiological laboratory. This is a retrospective observational study of BactAlert reactive blood cultures (n = 160) from Helgeland Hospital, July–December 2021, analysed by BCID2 locally and conventional culture at a regional laboratory
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Inhibiting pathogenicity of vaginal Candida albicans by lactic acid bacteria and MS analysis of their extracellular compounds APMIS (IF 2.8) Pub Date : 2024-01-02 Shreaya Das, Bolin Kumar Konwar
Maintaining healthy vaginal microflora post-puberty is critical. In this study we explore the potential of vaginal lactic acid bacteria (LAB) and their extracellular metabolites against the pathogenicity of Candida albicans. The probiotic culture free supernatant (PCFS) from Lactobacillus crispatus, L. gasseri, and L. vaginalis exhibit an inhibitory effect on budding, hyphae, and biofilm formation
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Rifampicin does not reduce moxifloxacin concentrations at the site of infection and may not improve treatment outcome of a one-stage exchange surgery protocol of implant-associated osteomyelitis lesions in a porcine model APMIS (IF 2.8) Pub Date : 2023-12-28 Sofus Vittrup, Louise Kruse Jensen, Katrine Top Hartmann, Bent Aalbæk, Pelle Hanberg, Josefine Slater, Magnus Andreas Hvistendahl, Maiken Stilling, Nis Pedersen Jørgensen, Mats Bue
We aimed to evaluate moxifloxacin steady-state concentrations in infected bone and soft tissue and to explore the additive microbiological and pathological treatment effect of rifampicin to standard moxifloxacin treatment of implant-associated osteomyelitis (IAO). 16 pigs were included. On Day 0, IAO was induced in the proximal tibia using a susceptible Staphylococcus aureus strain. On Day 7, the pigs
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Impact of RYGB surgery on plasma immunoglobulins: association between blood pressure and glucose levels six months after surgery APMIS (IF 2.8) Pub Date : 2023-12-27 Natalie Happonen, Mari-Anne Härma, Ramin Akhi, Antti E. Nissinen, Markku J. Savolainen, Maija Ruuth, Katariina Öörni, Krishna Adeshara, Markku Lehto, Per-Henrik Groop, Vesa Koivukangas, Janne Hukkanen, Sohvi Hörkkö
We aimed to study levels of natural antibodies in plasma, and their associations to clinical and fecal biomarkers, before and 6 months after Roux-en-Y gastric bypass (RYGB) surgery. Thirty individuals with obesity [16 type 2 diabetic, 14 non-diabetic (ND)] had RYGB surgery. Total plasma IgA, IgG and IgM antibody levels and specific antibodies to oxidized low-density lipoprotein (oxLDL), malondialdehyde-acetaldehyde
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Retraction: ‘Curcumin inhibits the survival and metastasis of prostate cancer cells via the Notch-1 signaling pathway’, by Jingzhe Yang, Chengli Wang, Zhijie Zhang, Xiaojun Chen, Yusen Jia, Bin Wang, Tao Kong, APMIS. 2017; 125: 134–140: The above article, published online on 24 January 2017 in Wiley Online Library (https://onlinelibrary.wiley.com/doi/10.1111/apm.12650) has been retracted by agreement APMIS (IF 2.8) Pub Date : 2023-12-26
The retraction has been agreed due to unattributed overlap between this article and the following article: ‘Curcumin inhibits cell survival and migration by suppression of Notch-1 activity in prostate cancer cells’ by Tao Kong, Yongxing Wang, Li Xiao and Limin Liao; African Journal of Pharmacy and Pharmacology, Vol.7(27), pp. 1911-1916, 2013, https://doi.org/10.5897/AJPP2012.1526. The authors were
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Evaluation of transcription factors and cytokine expressions of T-cell subsets in CD19 deficiency and their possible relationship with autoimmune disease APMIS (IF 2.8) Pub Date : 2023-12-14 Serkan Küççüktürk, Mehmet Ali Karaselek, Tuğçe Duran, İsmail Reisli
CD19 deficiency is a rare, predominantly antibody deficiency, and there are few studies showing that it can be seen in autoimmune diseases. The aim of study was evaluated to transcription factor and cytokine expressions of helper T (Th)-cell subsets in CD19 deficiency and the possible mechanism role of this factor expression in autoimmune disease. Transcription factor and cytokine expressions of Th1
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Short communication: first case of bacteraemia caused by Dielma fastidiosa in a patient hospitalized with diverticulitis APMIS (IF 2.8) Pub Date : 2023-12-14 Barbara Forman-Ankjær, Frederik Hvid-Jensen, Carl Mathias Kobel, Thomas Greve
Dielma fastidiosa is a gram-negative, anaerobic rod belonging to the family Erysipelotrichaceae. D. fastidiosa has previously been isolated in human stool samples as part of the commensal flora; however, prior to this case, it has never been identified as a human pathogen. We present the first case of bacteraemia with D. fastidiosa. Bacterial growth in the blood culture bottle was detected by the automated
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Anemia in liver transplant recipients: prevalence, severity, risk factors, and survival APMIS (IF 2.8) Pub Date : 2023-12-11 Nina Øksnes Hegland, Omid Rezahosseini, Christian Ross Pedersen, Dina Leth Møller, Terese Brun Bugge, Neval Ete Wareham, Nicoline Stender Arentoft, Jens Hillingsø, Thomas Kromann Lund, Allan Rasmussen, Susanne Dam Nielsen
Information about anemia in liver transplant (LTx) recipients is scarce. We investigated the prevalence and severity of anemia before and within the first-year post-LTx, risk factors for having anemia before LTx, and 1-year survival according to anemia status before LTx. This retrospective cohort study received data from The Knowledge Center for Transplantation database at Rigshospitalet, Copenhagen
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Bacteriophage-based approach for treatment of urinary tract infections: a quick outlook APMIS (IF 2.8) Pub Date : 2023-11-29 Bartłomiej Grygorcewicz, Marta Gliźniewicz, Joanna Jabłońska, Adrian Augustyniak, Patrycja Olszewska, Bartosz Wojciuk, Dominika Miłek, Natalia Serwin, Artur Czajkowski, Elżbieta Cecerska-Heryć, Rafał Rakoczy, Aneta Cymbaluk-Płoska
Urinary tract infections (UTIs) are among the most common bacterial infections affecting millions worldwide. The increasing emergence of antibiotic-resistant bacteria has become a serious concern in managing UTIs. Therefore, there is a growing interest in using bacteriophages as an alternative or adjunct therapy for UTIs. Bacteriophages are viruses that infect and kill bacteria, making them a promising
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The microbiome in the pathogenesis of lung cancer APMIS (IF 2.8) Pub Date : 2023-11-16 Sainan Miao, Huan Qiu
As one of the malignant tumors with high incidence rate and high mortality, lung cancer seriously threatens the life safety of patients. Research shows that microorganisms are closely related to lung cancer. The microbiome is symbiotic with the host and plays a vital role in the functions of the human body. Microbiota dysbiosis is correlated with development of lung cancer. However, the underlying
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Correction to “The relevance of ERG immunoexpression intensity for prostatic adenocarcinoma in radical prostatectomy of 635 samples” APMIS (IF 2.8) Pub Date : 2023-11-16
Feitosa, P.M.F.A, Hirth, C.G., Silva-Fernandes, I.J.D.L. and Dornelas, C.A. (2023). The relevance of ERG immunoexpression intensity for prostatic adenocarcinoma in radical prostatectomy of 635 samples. APMIS, 131: 465–471. https://doi.org/10.1111/apm.13330 The 4th author affiliation were incomplete and have been added. The updated author byline and affiliations are listed below. PRISCILLA MARIANA FREITAS
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Antibiotic resistances of Pseudomonas aeruginosa and Acinetobacter baumannii in urine cultures: experience in a hospital of Southeast Spain APMIS (IF 2.8) Pub Date : 2023-11-16 Horacio Requena-Cabello, Enrique Rodríguez-Guerrero, Manuela Expósito-Ruiz, José María Navarro-Marí, Jose Gutierrez-Fernandez
The objectives of this study were to perform a systematic review of publications between 2010 and 2021 on the antibiotic resistance of Pseudomonas aeruginosa and Acinetobacter baumannii from urinary tract infections and to analyze changes over time in hospital urine cultures from 2016 through 2021. The literature was searched, and a retrospective cross-sectional descriptive study was performed in the
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Human lung vascular endothelium may limit infection with HRV16 via IFN-β-dependent mechanisms APMIS (IF 2.8) Pub Date : 2023-11-16 Mateusz Gawrysiak, Robert Szewczyk, Mateusz Kobierecki, Michał Szymański, Adrian Gajewski, Izabela Gulbas, Sylwia Michlewska, Maciej Chałubiński
Human rhinovirus 16 (HRV16) may induce inflammatory and antiviral responses in the human lung vascular endothelium (ECs) and impair its barrier functions after infection. However, ECs may regain barrier and metabolic functions. Mechanisms of limitation of HRV16 infection in the lung vascular endothelium are unknown. Human lung vascular endothelium (HMVEC-L) was infected with HRV16. IFN-β, OAS-1, and
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Antimicrobial susceptibilities and genomic characteristics of methicillin-resistant Staphylococcus aureus resistant to mupirocin in Stockholm, Sweden APMIS (IF 2.8) Pub Date : 2023-11-15 Nora Vestberg, Mohammad Razavi, Christian G. Giske, Hong Fang
The aim of this study was to investigate antimicrobial susceptibilities and genomic characteristics of mupirocin-resistant MRSA isolates in Stockholm, Sweden. In total, 44 non-duplicate mupirocin-resistant MRSA isolates detected in Stockholm during 2010–2022 were investigated. Antimicrobial susceptibility testing was performed using broth microdilution method and further tested for high-level mupirocin-resistance
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Role and new insights of microfibrillar-associated protein 4 in fibrotic diseases APMIS (IF 2.8) Pub Date : 2023-11-13 Long Zhu, Wenqun Gou, Lijia Ou, Binjie Liu, Manyi Liu, Hui Feng
Fibrosis is one of the most worrisome complications of chronic inflammatory diseases, leading to tissue damage, organ failure, and ultimately, death. The most notable pathological characteristic of fibrosis is the excessive accumulation of extracellular matrix (ECM) components such as collagen and fibronectin adjacent to foci of inflammation or damage. The human microfibrillar-associated protein 4
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Vaginal microecology and its role in human papillomavirus infection and human papillomavirus associated cervical lesions APMIS (IF 2.8) Pub Date : 2023-11-08 Jiatian Ye, Xiaorong Qi
The vaginal microecology comprises the vaginal microbiome, immune microenvironment, vaginal anatomy, and the cervicovaginal fluid, which is rich in metabolites, enzymes, and cytokines. Investigating its role in the female reproductive system holds paramount significance. The advent of next-generation sequencing enabled a more profound investigation into the structure of the vaginal microbial community
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Metabolic syndrome, soluble CD40L, and biomarkers of endothelial dysfunction in people living with HIV APMIS (IF 2.8) Pub Date : 2023-10-17 Mads-Holger B. Jacobsen, Andreas D. Knudsen, Thomas Benfield, Sisse R. Ostrowski, Shoaib Afzal, Edith W. Sørensen, Susanne D. Nielsen, Marco Gelpi
In the present study, we aimed to investigate the association between soluble CD40 ligand (sCD40L, a marker of platelet activation), soluble thrombomodulin, and syndecan-1 (both well-described markers of endothelial dysfunction) and metabolic syndrome in a large cohort of well-treated people with HIV (PWH) and to elucidate their association with HIV-specific variables. We included 862 PWH with undetectable
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Polyphyllin I, a strong antifungal compound against Candida albicans APMIS (IF 2.8) Pub Date : 2023-09-27 Yu Zhang, Jingxiao Zhang, Jian Sun, Min Zhang, Xin Liu, Longfei Yang, Yongjie Yin
This study was performed to explore the antifungal and antibiofilm effects of polyphyllin I (PPI) on Candida albicans. Microdilution assay was performed to determine the minimal inhibitory concentrations (MIC) of PPI against Candida species. Adhesion assay, hyphal growth assay, biofilm formation, and development were used to test the impacts of PPI on C. albicans virulence factors. Propidium iodide
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Does the route matter? A preclinical review of mesenchymal stromal cell delivery to the kidney APMIS (IF 2.8) Pub Date : 2023-09-25 Jean-Claude Kresse, Emil Gregersen, Jasmine Cicek Leifing Atay, Marco Eijken, Rikke Nørregaard
Mesenchymal stromal/stem cell (MSC) therapy has been thoroughly tested in preclinical animal models and holds great promise for the treatment of kidney diseases. It is becoming increasingly evident that the efficacy of MSC therapy is dependent on several factors including dosage, the tissue source of MSCs, the route of delivery and timing of administration. In a time where MSC therapy is moving from
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Single cells and bacterial biofilm populations in chronic wound infections APMIS (IF 2.8) Pub Date : 2023-09-17 Mads Lichtenberg, Klaus Kirketerp-Møller, Lasse A. Kvich, Mads Holm Christensen, Blaine Fritz, Tim Holm Jakobsen, Thomas Bjarnsholt
Chronic wounds and chronic ulcers are an increasing problem associated with high health care burden and patient burden. The arrested healing of chronic wounds has, in part, been attributed to the presence of biofilms. Substantial research has documented the presence of biofilms in chronic wounds, and many mechanisms of host–pathogen interactions have been uncovered to explain the arrested healing.
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Good's syndrome: brief overview of an enigmatic immune deficiency APMIS (IF 2.8) Pub Date : 2023-09-20 Ferenc Sipos, Györgyi Műzes
Good's syndrome, an infrequent adult-onset immunodeficiency is characterized by the triad of thymoma, hypogammaglobulinemia, and increased susceptibility to recurrent infections. The clinical presentation is highly variable, with a spectrum ranging from recurrent bacterial and opportunistic infections to concomitant autoimmune diseases and, sometimes malignant pathologies. Due to heterogeneous clinical
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APMIS focus issue 2023-fungal infections: diagnostics and therapeutics. APMIS (IF 2.8) Pub Date : 2023-09-15 Gordon Ramage,Åse Bengård Andersen
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Are we ready for translational research based on material and data from the Danish CancerBiobank and can we gain new knowledge from biobank registration? APMIS (IF 2.8) Pub Date : 2023-08-31 Estrid V.S. Høgdall, Ib Jarle Christensen, Claus Høgdall
Bio-and GenomeBank, Denmark (RBGB) is a nationwide infra-structure. Danish CancerBiobank (DCB) is a biobank in RBGB. The aim is to describe the degree of biological material collected and stored in DCB for patients diagnosed with primary ovarian cancer registered in The Danish Gynecologic Cancer Database (DGCD). Furthermore, to investigate the concordance between predicted organ of disease registered
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No evidence of increased anti-M3 muscarinic acetylcholine receptor autoantibodies in SSA-positive connective tissue disease patients APMIS (IF 2.8) Pub Date : 2023-08-24 Elif Hatipoğlu, Jakob Hauge Mikkelsen, Trine-Line Korsholm, Malene Hvid, Bent Deleuran, Marie Louise Næstholt Dahl
Anti-muscarinic type 3 receptor autoantibodies (M3R) and anti-SSA antibodies are both related to salivary secretion. The presence of M3R antibodies in Sjögren's syndrome is previously demonstrated; nevertheless, the relationship between the anti-SSA antibodies and M3R fragment antibodies, namely the N terminal, first, second, and third extracellular loops, remains to be elucidated. In this study, we
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Web-grading—a tool to test personal grading of renal and prostate cancer APMIS (IF 2.8) Pub Date : 2023-08-24 Glen Kristiansen, Matthias Schmid, Lars Egevad, Hemamali Samaratunga, Murali Varma, Kaan Inam, Hans-Jürgen Thiesen, Brett Delahunt, Yulin Dai
Only a few pathologists have the opportunity to verify their personal grading through objective assessment. This study introduces a web-based grading platform to facilitate and validate the grading of renal cell carcinoma and prostate cancer. Two representative images of two clinically annotated cohorts of 100 cases each of prostate and renal cell carcinoma were used. Each participant was asked to
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Prevalence, molecular markers, and outcome of bronchial squamous carcinoma in situ in high-risk subjects APMIS (IF 2.8) Pub Date : 2023-08-23 Steinn Jonsson, Wilbur A. Franklin, Marileila Varella-Garcia, Timothy C. Kennedy, Daniel Merrick, Kathryn D. Matney, Gudrun N. Oskarsdottir, Arni Saemundsson, Robert L. Keith, Paul A. Bunn, York E. Miller
Bronchial squamous carcinoma in situ (CIS) is a preinvasive lesion that is thought to precede invasive carcinoma. We conducted prospective autofluorescence and white light bronchoscopy trials between 1992 and 2016 to assess the prevalence, molecular markers, and outcome of individuals with CIS and other preneoplastic bronchial lesions. Biopsies were evaluated at multiple levels and selected biopsies
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Performance of automated antimicrobial susceptibility testing for the detection of antimicrobial resistance in gram-negative bacteria: a NordicAST study APMIS (IF 2.8) Pub Date : 2023-08-17 Truls M. Leegaard, Ulrik S. Justesen, Erika Matuschek, Christian G. Giske
Automated testing of antimicrobial susceptibility is common in clinical microbiology laboratories but their ability to detect low-level resistance has been questioned. This Nordic multicentre study aimed to evaluate the performance of commercially available automated AST systems. A phenotypically well-characterised collection of gram-negative bacilli (Escherichia coli (n = 7), Klebsiella pneumoniae
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High seroprevalence of SARS-CoV-2 antibodies in healthcare workers in COVID-19 wards indicates an occupational hazard—a prospective cohort study during the first year of the COVID-19 pandemic in Kalmar County, Sweden APMIS (IF 2.8) Pub Date : 2023-07-18 Viktor Hultqvist, Jonas Bonnedahl, Jonathan Edwardsson, Ivar Tjernberg, Ingvar Rydén
The aim of this study is to report the seroprevalence of SARS-CoV-2 antibodies in healthcare workers with various risk of occupational exposure in Kalmar County, Sweden, during the first year of the coronavirus disease 2019 (COVID-19) pandemic. We performed SARS-CoV-2 antibody measurements at four time points, from May 2020 to May 2021, in 401 healthcare workers (HCW) at seven hospital wards and two
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Predicting the clinical efficacy of JAK inhibitor treatment for patients with rheumatoid arthritis based on Fas+ T cell subsets APMIS (IF 2.8) Pub Date : 2023-07-13 Shan-Wen Lui, Ting-Yu Hsieh, Jeng-Wei Lu, Yen-Chen Chen, Ting-Chun Lin, Yi-Jung Ho, Feng-Cheng Liu
Rheumatoid arthritis (RA) is a common autoimmune disease. Janus kinase inhibitors (JAKi) have been approved for the treatment of RA; however, the impact of JAKi on immune cells remains inconclusive. This study investigated the response of immune cells to JAKi treatment to identify biomarkers by which to evaluate and predict clinical outcomes. Blood samples were collected from RA patients before and
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The relevance of ERG immunoexpression intensity for prostatic adenocarcinoma in radical prostatectomy of 635 samples APMIS (IF 2.8) Pub Date : 2023-07-13 Priscilla Mariana Freitas Aguiar Feitosa, Carlos Gustavo Hirth, Isabelle Joyce De Lima Silva-Fernandes, Conceição Aparecida Dornelas
Prostate cancer is the world's most frequently diagnosed malignancy in men. Recent work suggests that patients with high ERG expression intensity are significantly more likely to develop biochemical relapse and metastasis, and die of prostate cancer. The objective of this study was to determine the relationship between the intensity of ERG protein expression and the staging of prostate cancer and the
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Appraisal of the PathoDiscovery: an interactive web-based educational tool for teaching pathophysiology and histopathology APMIS (IF 2.8) Pub Date : 2023-07-07 Alessandra I.G. Buma, Femke Simmer, Marjanne den Braber-Ymker, Patricia J.T.A. Groenen
Virtual pathology education has shown to enhance the students' learning experience. At the Radboud University, an E-learning platform—called the “PathoDiscovery”—was developed and first used in a course about neoplasm development amongst first year (bio)medical sciences students. The PathoDiscovery incorporates high-power microscopic images, histological annotations, interactive questions and pre-programmed
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Dynamic distribution of gut microbiota during Alzheimer's disease progression in a mice model APMIS (IF 2.8) Pub Date : 2023-06-26 Jianshen Zhu, Shuyun Liu, Haoran Zhang, Wenjing Zhao, Jinmei Ding, Ronghua Dai, Ke Xu, Chuan He, Jiajia Liu, Lingyu Yang, He Meng
Alzheimer's disease (AD) is an irreversible neurodegenerative disease that affects more than 44 million people worldwide. The pathogenic mechanisms of AD still remain unclear. Currently, there are numerous studies investigating the microbiota–gut–brain axis in humans and rodents indicated that gut microbiota played a role in neurodegenerative diseases, including AD. However, the underlying relationship
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Pre-existing, treatment-specific resistance-associated substitutions in hepatitis C virus genotype 1 and 3 and viral RNA titers during treatment with direct-acting antivirals APMIS (IF 2.8) Pub Date : 2023-06-25 Christina Sølund, Martin S. Pedersen, Ulrik Fahnøe, Jonathan Filskov, Håvard Jenssen, Nina Weis, Kristian Schønning, Jens Bukh
The introduction of direct-acting antiviral (DAA) treatment of hepatitis C virus (HCV) infected patients has greatly increased treatment success rates. However, viral response kinetics to DAA treatment may depend on pre-existing resistance-associated substitutions (RASs) in HCV. The aim of this study was to describe how pre-existing RASs affect DAA treatment-induced reduction in HCV RNA titers in HCV
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Antibiotic resistance and biofilm-forming ability of α-toxin-positive Clostridium septicum isolates worsen patient prognosis APMIS (IF 2.8) Pub Date : 2023-06-25 Jozef Kuzma, Sofía Guadalupe Zavala-Meneses, Ludovit Skultety, Dittmar Chmelar, Jozef Ficík, Lenka Palcová
A total of, 78 Clostridium septicum (CLSE) isolates were screened for genes encoding: α-toxin, flagellin, and resistance to vancomycin (VANg). The isolates were also tested for their ability to form biofilm and their antibiotic susceptibility. All isolates were positive for α-toxin and flagellin genes. However, only 19 isolates (24.3%) showed prevalence for VANg. We observed the strongest capacity
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Azole resistance in Candida auris: mechanisms and combinatorial therapy APMIS (IF 2.8) Pub Date : 2023-06-20 Puneet Jangir, Sapna Kalra, Sunita Tanwar, Vinay Kumar Bari
Multidrug resistance Candida auris is a dangerous fungal pathogen that is emerging at an alarming rate and posing serious threats to public health. C. auris is associated with nosocomial infections that cause invasive candidiasis in immunocompromised patients. Several antifungal drugs with distinct mechanisms of action are clinically approved for the treatment of fungal infections. The high rates of
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Screening the medicine for malaria venture's Pandemic Response Box to identify novel inhibitors of Candida albicans and Candida auris biofilm formation APMIS (IF 2.8) Pub Date : 2023-06-20 Olabayo H. Ajetunmobi, Ashok K. Chaturvedi, Hamid Badali, Alessandra Vaccaro, Laura Najvar, Floyd L. Wormley, Nathan P. Wiederhold, Thomas F. Patterson, Jose L. Lopez-Ribot
Candida spp. are opportunistic yeasts capable of forming biofilms, which contribute to resistance, increasing the urgency for new effective antifungal therapies. Repurposing existing drugs could significantly accelerate the development of novel therapies against candidiasis. We screened the Pandemic Response Box containing 400 diverse drug-like molecules active against bacteria, viruses or fungi, for
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Chondromatous metaplasia within native cardiac valves: clinicopathologic characterization with particular focus upon aortic valves APMIS (IF 2.8) Pub Date : 2023-06-19 Simona Pichler Sekulic, Miroslav Sekulic
Native cardiac valves in the setting of chronic injury may become thickened and disrupted by dystrophic calcification, which impede valve structure/function, and there may be evidence of chondromatous (i.e., cartilaginous, CM) metaplasia admixed with dystrophic calcification. In order to characterize the presence of CM in native cardiac valves – with particular focus upon aortic valves – a retrospective
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Louis Pasteur—The life of a controversial scientist with a prepared mind, driven by curiosity, motivation, and competition APMIS (IF 2.8) Pub Date : 2023-06-13 Carl-Henrik Brogren
Louis Pasteur, born December 27, 1822 in Dole, France, showed in his childhood and youth great abilities as an artistic painter; however by an age of 19, his interest changed toward science, and he moved to Paris to study chemistry and physics at École Normale Supérieure. During graduation, he initiated research on chiral crystallography and stereochemistry and got his doctorates in 1847 in both chemistry
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Antimicrobial resistance of Pseudomonas aeruginosa in a cystic fibrosis population after introduction of a novel cephalosporin/β-lactamase inhibitor combination APMIS (IF 2.8) Pub Date : 2023-06-09 Terese L. Katzenstein, Daniel Faurholt-Jepsen, Tavs Qvist, Peter Østrup Jensen, Tacjana Pressler, Helle Krogh Johansen, Mette Kolpen
Ceftolozane-tazobactam is a new β-lactam/β-lactamase inhibitor combination approved by the U.S. Food and Drug Administration in 2019 for the treatment of hospital-acquired and ventilator-associated pneumonia. The combination is a particularly potent inhibitor of penicillin-binding proteins with higher affinity than other β-lactam agents. Persons with cystic fibrosis (pwCF) often harbour resistant Gram-negative
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E-cadherin and aquaporin-3 are downregulated in wound edges of human chronic wounds APMIS (IF 2.8) Pub Date : 2023-06-02 Christina V. Ernstsen, Andreas Riishede, Anne Kristine S. Iversen, Lene Bay, Thomas Bjarnsholt, Lene N. Nejsum
Chronic wounds are defined as wounds that fail to proceed through the normal phases of wound healing; a complex process involving different dynamic events including migration of keratinocytes in the epidermis. Chronic wounds are estimated to affect 1–2% of the human population worldwide and are a major socioeconomic burden. The prevalence of chronic wounds is expected to increase with the rising number