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CD14 + HLADR - blood values in patients after alloHSCT are highly predictive of survival and infectious complications Transpl. Immunol. (IF 1.624) Pub Date : 2021-01-20 Emilia Jaskuła; Janusz Lange; Mariola Sędzimirska; Krzysztof Suchnicki; Monika Mordak-Domagała; Helena Pakos; Andrzej Lange
Background Cytokine storm described in patients after allogeneic haematopoietic stem cell transplantation (alloHSCT) is associated with the appearance of CD14 + HLADR - in the blood. Methods To study the role of CD14 + HLADR - cells 223 patients after alloHSCT followed from 1 month to 15 years. The methods used included flow cytometry for blood cells profiling, nucleic acid tests for viral reactivation
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Eplet-based virtual PRA increases transplant probability in highly-sensitized patients Transpl. Immunol. (IF 1.624) Pub Date : 2021-01-09 Sandra Tafulo; Jorge Malheiro; Leonídio Dias; Manuela Almeida; La Salete Martins; Sofia Pedroso; Ermelinda Osório; Luísa Lobato; António Castro-Henriques
Background: The reduced access of highly-sensitized (HS) patients to kidney transplantation (KTx) is one of the major challenges for transplant community. Therefore, the aim of our study was to estimate the impact of three different vPRA calculations, assessed traditionally and using eplet-based analysis, in donor offers. Methods: At 01-01-2020, 157 HS patients are waitlisted for deceased donor KTx
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Impact of rituximab on the T-cell flow cytometric crossmatch Transpl. Immunol. (IF 1.624) Pub Date : 2020-12-22 Sam Arul Doss; Siddharth Mittal; Dolly Daniel
Rituximab is frequently used in the setting of ABO-incompatible renal transplants, and highly sensitized patients. Its interference with B-cell flow cytometric crossmatch (B-FCXM) is well known. However, its effect on the T-cell flow cytometric crossmatch (T-FCXM) has not been described. We aimed to evaluate the effect of rituximab on the T-FCXM using non-pronase and pronase treated donor lymphocytes
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Cytomegalovirus chemokine receptor M33 knockout reduces chronic allograft rejection in a murine aortic transplant model Transpl. Immunol. (IF 1.624) Pub Date : 2020-12-07 Niklas M. Fritz; Thomas Stamminger; Martina Ramsperger-Gleixner; Annika V. Kuckhahn; Regina Müller; Michael Weyand; Christian Heim
Background Numerous studies suggest that cytomegalovirus (CMV) infection may act as isolated risk factor in the development of cardiac allograft vasculopathy (CAV). Viral G protein-coupled receptors (GPCRs) are thought to contribute to the pathogenic changes associated with CMV infection. The aim of this study was to investigate the role of murine cytomegalovirus GPCR M33 in the development of CAV
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A post-preservation vascular flush removes significant populations of donor leukocytes prior to lung transplantation Transpl. Immunol. (IF 1.624) Pub Date : 2020-11-29 Alexandra L. Ball; Rebecca J. Edge; Kavit Amin; William R. Critchley; Gareth J. Howell; Nizar Yonan; John P. Stone; James E. Fildes
Background Donor leukocytes are intrinsically involved in acute lung allograft rejection, via self-presentation of donor antigens to recipient leukocytes. Therapeutic modalities to remove donor leukocytes are currently unavailable. We evaluated if a vascular flush immediately following preservation can be used for this purpose. Methods A post-preservation flush was performed with STEEN solution in
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A Europe wide acceptable mismatch program will enable transplantation of long waiting highly sensitised patients with a compatible donor Transpl. Immunol. (IF 1.624) Pub Date : 2020-12-01 Lisa Mumford; Susan V. Fuggle; Jaume Martorell; Antonij Slavcev; Aliki Iniotaki; Geert W. Haasnoot; Sebastiaan Heidt; Frans H.J. Claas
Immunisation against Human Leucocyte Antigens (HLA) can be caused by pregnancy, blood transfusion, or organ transplants. The HLA antibody status of a given patient significantly influences their access and waiting time to transplant. For some highly sensitised patients (HSP) there is hardly any suitable donor available in the deceased donor pool of their allocation organisation and therefore they wait
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Mycophenolate mofetil preconditioning protects mouse liver against ischemia/reperfusion injury in wild type and toll-like receptor 4 knockout and mice Transpl. Immunol. (IF 1.624) Pub Date : 2020-12-03 Cheng Xiong; Zhendong Du; Youwei Zhu; Meilin Xue; Yongsheng Jiang; Yiming Zhong; Lingxi Jiang; Hao Chen; Minmin Shi
Background Mycophenolate mofetil (MMF), an immunosuppressive drug, exerts anti-inflammatory effects on organs during ischemia/reperfusion (I/R) injury. However, the exact function of MMF in hepatic I/R injury remains largely unknown. The purpose of this study was to explore the role and potential mechanism of MMF protection in hepatic I/R injury. Methods Male wild type (WT) and TLR4 knockout (KO) mice
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Immunosuppressant-induced cutaneous drug reactions in solid organ transplant recipients Transpl. Immunol. (IF 1.624) Pub Date : 2020-11-29 Isadora Zago Miotto; Bruno de Castro e Souza; Stephen K. Tyring; Walmar Roncalli Pereira de Oliveira
Solid organ transplant recipients (SOTRs) are susceptible to various cutaneous side effects as a consequence of long-term immunosuppressive therapy. Skin cancers and infections are well-studied complications that can cause death and/or allograft rejection. Other cutaneous drug reactions, such as inflammatory manifestations, have a high prevalence but are rarely studied. We analyzed these manifestations'
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Free Airway C4d after Lung Transplantation - A Quantitative Analysis of Bronchoalveolar Lavage Fluid Transpl. Immunol. (IF 1.624) Pub Date : 2020-11-17 Tobias Heigl; Berta Saez-Gimenez; Anke Van Herck; Janne Kaes; Annelore Sacreas; Hanne Beeckmans; Gene P.L. Ambrocio; Hanneke Kwakkel-Van Erp; Sofie Ordies; Arno Vanstapel; Stijn E. Verleden; Arne P. Neyrinck; Laurens J. Ceulemans; Dirk E. Van Raemdonck; Erik Verbeken; Geert M. Verleden; Robin Vos; Bart Vanaudenaerde
In recent years, the utility of vascular complement factor 4d (C4d) deposition as diagnostic tool for antibody mediated rejection (AMR) after lung transplantation, has become a controversial issue. We aimed to pinpoint the problematic nature of C4d as biomarker with a simple experiment. We quantified C4d in broncho-alveolar lavage (BAL) of lung transplant patients with diverse post-transplant complications
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Implications of macrophage polarization in corneal transplantation rejection Transpl. Immunol. (IF 1.624) Pub Date : 2020-11-22 Huiwen Tian; Jing Wu; Ming Ma
Corneal transplantation rejection remains an urgent problem threatening the success rate of high-risk patients. Macrophages are involved in the rejection of corneal transplants. Macrophages have M1 and M2 phenotypes, classified according their response to external stimuli. Macrophage polarization, through which these distinct forms are activated, is not only involved in the occurrence and development
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Soluble CD83 inhibits acute rejection by up regulating TGF-β and IDO secretion in rat liver transplantation Transpl. Immunol. (IF 1.624) Pub Date : 2020-11-08 Liangxing Xiong; Danxin Wang; Shibu Lin; Yubin Wang; Mingwei Luo; Lianghui Gao
Background Allogeneic transplantation immune tolerance is currently a hot research issue and soluble CD83(sCD83) is a novel immunomodulator with great potential in inducing transplantation tolerance. Objective To investigate the mechanism of the immune tolerance effect of sCD83 on rat liver transplantation. Method A rat liver transplantation model was established to study the effects of sCD83 on the
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The challenge of using the virtual crossmatch as a singular tool for the detection of Anti-HLA antibodies- A study from a tertiary care institute from South India Transpl. Immunol. (IF 1.624) Pub Date : 2020-10-28 Snehil Kumar; Sam Arul Doss; S. Stephen; M. Pratheeba; L. Jeyaseelan; Dolly Daniel
Introduction Detection of donor specific antibodies (DSA) is critical in both solid organ and mismatched haematopoetic stem cell transplants. The single antigen bead assay (SAB) is widely used as a virtual crossmatch in these settings. However, HLA allele variation across ethnicities and differing genetic backgrounds is a well-known and acknowledged fact and representation of alleles prevalent in a
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Delayed non-myeloablative irradiation to induce long-term allograft acceptance in a large animal lung transplantation model Transpl. Immunol. (IF 1.624) Pub Date : 2020-10-27 Karolin S. Hacker; Katharina Jansson; Jeanette Pichler; Jawad Salman; Murat Avsar; Thierry Siemeni; Ann-Kathrin Knöfel; Klaus Höffler; Jens Gottlieb; Jörg Frühauf; Martin Werner; Reza Poyanmehr; Danny Jonigk; Michael S. Balzer; Marion Hewicker-Trautwein; Axel Haverich; Wiebke Sommer; Gregor Warnecke
We previously induced long-term allograft acceptance in an allogeneic lung transplantation (LTx) model in miniature swine using perioperative non-myeloablative irradiation (IRR) combined with infusion of donor specific alloantigen. In order to improve clinical applicability, we delayed induction with irradiation in this study. Left sided single LTx was performed in minipigs. Group 1 received non-myeloablative
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Development and application of a massive open online course to deliver innovative transplant education Transpl. Immunol. (IF 1.624) Pub Date : 2020-10-24 Peter G.M. de Jong; Renée A. Hendriks; Franka Luk; Augusto Cesar Soares dos Santos; Marlies E.J. Reinders
Massive Open Online Courses (MOOCs) offer an entirely new course concept for delivering content and engaging learners. This method of teaching has huge potential for the field of transplant education. In this study we describe the development and implementation of the MOOC “Clinical Kidney, Pancreas and Islet Transplantation”. Three and a half years after the introduction of the course, the learning
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Fingolimod (FTY720) prevents chronic rejection of rodent cardiac allografts through inhibition of the RhoA pathway Transpl. Immunol. (IF 1.624) Pub Date : 2020-10-24 Wei Chen; Wenhao Chen; Song Chen; Ahmed Uosef; Rafik M. Ghobrial; Malgorzata Kloc
The Fingolimod (FTY720, Gilenya) is clinically approved for the treatment of multiple sclerosis (MS). Its therapeutic effect on MS is based on the ability to bind sphingosine 1-phosphate (S1P) receptors and block the exit of immune cells from the lymphoid organs, thus preventing immune cell-dependent injury to the central nervous system (CNS). We showed recently that, besides the S1P-related activity
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Blockade of T cell activation induced by the simultaneous absence of Nod1 and Nod2 is bypassed by TLR2 signals Transpl. Immunol. (IF 1.624) Pub Date : 2020-10-24 Sashi Kasimsetty; Alana Hawkes; Sean E. DeWolf; Alexander Welch; Dianne B. McKay
Pattern recognition receptors (PRRs) trigger adaptive inflammatory responses and as such are attractive targets for therapeutic manipulation of inflammation. In order to develop effective therapies however we need to understand the complexities of PRR signaling and clarify how individual PRRs contribute to an inflammatory response in a given cell type. Data from our lab and others have shown that cross-talk
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Donor specific anti-HLA antibodies and cardiac allograft vasculopathy: A prospective study using highly automated 3-D optical coherence tomography analysis Transpl. Immunol. (IF 1.624) Pub Date : 2020-10-15 Pazdernik Michal; Bedanova Helena; Chen Zhi; Kautzner Josef; Melenovsky Vojtech; Malek Ivan; Slavcev Antonij; Bartonova Michaela; Karmazin Vladimir; Eckhardt Tomas; Tomasek Ales; Ozabalova Eva; Kovarnik Tomas; Wohlfahrt Peter; Sonka Milan
Introduction Recent studies suggested potential positive correlations between HLA-specific antibodies and development of cardiac allograft vasculopathy (CAV). Methods This prospective two-center study investigated early progression of CAV by coronary optical coherence tomography in 1 month and 12 months after heart transplantation (HTx) in 104 patients. Detection and characterization of donor specific
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Triazolopyrimidine derivative NK026680 and donor-specific transfusion induces CD4+CD25+Foxp3+ T cells and ameliorates allograft rejection in an antigen-specific manner Transpl. Immunol. (IF 1.624) Pub Date : 2020-10-03 Shin Emoto; Susumu Shibasaki; Akihisa Nagatsu; Ryoichi Goto; Hitoshi Ono; Yasutomo Fukasaku; Rumi Igarashi; Takuji Ota; Moto Fukai; Tsuyoshi Shimamura; Kan Saiga; Akinobu Taketomi; Masaaki Murakami; Satoru Todo; Kenichiro Yamashita
We have previously demonstrated the unique properties of a new triazolopyrimidine derivative, NK026680, which exerts immunosuppressive effects in rat heart transplant model and confers tolerogeneic properties on ex vivo-conditioned dendritic cells in mice. We herein demonstrate that NK026680 promotes the expansion of regulatory T cells (Tregs) with potent immunoregulatory effects when used in combination
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Immunosuppression in uterine transplantation. Transpl. Immunol. (IF 1.624) Pub Date : 2020-09-11 Rui Zhang,Shou-Long Deng,Zheng-Xing Lian,Kun Yu
Uterine transplantation (UTx) is the only effective treatment for uterine infertility patients to become genetic mothers. After decades of research, the surgical methods of UTx are very developed. There are numerous factors that affect the results of UTx, such as selection of the donor uterus before transplantation, immunosuppressive therapy post-transplantation, rejection monitoring, and immune tolerance
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Mechanisms underlying the production of chemokine CXCL11 in the reaction of renal tubular epithelial cells with CD4+ and CD8+ T cells Transpl. Immunol. (IF 1.624) Pub Date : 2020-09-21 Xiaoping Wang; Dan Wang; Xiao Wang; Xiaoqi Wang; Qingzhen Gao
Aim To study the release mechanism of C-X-C motif chemokine 11 (CXCL11) and other chemokines after the co-cultivation of CD4+ and CD8+ T cells with the renal tubular epithelial cells (RTEC) in the process of allograft renal transplantation rejection. Methods The Human CD4+, CD8+ T cells were obtained from the blood of volunteers and kidney transplantation (Ktx) patients, and co-cultured with renal
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Targeting glutamine metabolism as an effective means to promote allograft acceptance while inhibit tumor growth. Transpl. Immunol. (IF 1.624) Pub Date : 2020-09-13 Chen-Fang Lee,Chih-Hsien Cheng,Hao-Chien Hung,Kun-Ming Chan,Wei-Chen Lee
Recently the role of metabolic signaling pathways has emerged as playing a critical role in dictating the outcome of T cell responses. The uptake and metabolism of the amino acid glutamine is essential for effector T cell activation. Since the growth and expansion of tumor cells relies on similar anabolic and metabolic requirements, we hypothesized that glutamine blockage might represent a promising
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Investigating the relationship between class I HLA-specific immunoglobulin-G subclasses, Pan-IgG single antigen bead assays and complement mediated interference in sera from renal transplant recipients. Transpl. Immunol. (IF 1.624) Pub Date : 2020-09-12 Petra Goldsmith,David Lowe,Chang Wong,Dan Ridgway,Matthew Howse,Abdul Hammad,Sanjay Mehra,Steve Christmas,Andrew Jones
Introduction Antibody mediated rejection is the leading cause of kidney transplant failure. Not all antibodies are harmful and some may be protective. Immunoglulin Gs, of which there are four subtypes, are detected by single antigen bead testing. The aims of this study were to characterise the IgG subclass profiles for class I HLA-specific antibodies in an uncensored post-transplant population and
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Effects of re-augmenting maintenance immunosuppression on post-transplant donor-specific HLA antibodies in liver transplantation. Transpl. Immunol. (IF 1.624) Pub Date : 2020-09-10 Kazuaki Tokodai,Shigehito Miyagi,Wataru Nakanishi,Atsushi Fujio,Toshiaki Kashiwadate,Masafumi Goto,Michiaki Unno,Takashi Kamei
Background Donor-specific antibodies (DSAs) have various negative short- and long-term effects after organ transplantation. DSAs are prevalent in patients with insufficient immunosuppression; thus, even patients with stable conditions after liver transplantation should be under optimized immunosuppression. However, the effect of re-augmenting immunosuppression therapy for patients with insufficient
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Influence of pretransplant class I and II non-donor-specific anti-HLA immunization on immunologic outcome and graft survival in kidney transplant recipients. Transpl. Immunol. (IF 1.624) Pub Date : 2020-09-10 Anja Staeck,Dmytro Khadzhynov,Anna Kleinsteuber,Lukas Lehner,Michael Duerr,Klemens Budde,Nils Lachmann,Fabian Halleck,Oliver Staeck
Background Anti-HLA immunization determined by Panel Reactive Antibody (PRA) is known to have a negative impact on patient and graft survival. The predictive value of peak PRA (pPRA) on immunologic outcome, however, and the individual effects of anti-HLA class I and II antibodies remain uncertain. Methods The influence of HLA immunization on immunologic outcome parameters and graft survival was investigated
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Treg expansion with trichostatin A ameliorates kidney ischemia/reperfusion injury in mice by suppressing the expression of costimulatory molecules. Transpl. Immunol. (IF 1.624) Pub Date : 2020-09-04 Ryohei Yamamoto,Mitsuru Saito,Takuro Saito,Ryuichiro Sagehashi,Atsushi Koizumi,Taketoshi Nara,Sohei Kanda,Kazuyuki Numakura,Shintaro Narita,Takamitsu Inoue,Shigeru Satoh,Tomonori Habuchi
Innate immune reactions are believed to be associated with ischemia/reperfusion injury (IRI), and IRI might be treatable by expanding regulatory T cells (Tregs), which can suppress the excessive responses of the immune system. Organ IRI is known to be closely involved in the expression of costimulatory molecules. The present study aimed to assess whether Tregs endogenously expanded by the administration
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Sex-dependent mechanisms involved in renal tolerance to ischemia-reperfusion: Role of inflammation and histone H3 citrullination. Transpl. Immunol. (IF 1.624) Pub Date : 2020-09-03 Zesergio Melo,Yanet Karina Gutierrez-Mercado,David Garcia-Martínez,Eliseo Portilla-de Buen,Alejandro A Canales-Aguirre,Ricardo Gonzalez-Gonzalez,Adriana Franco-Acevedo,Julio Palomino,Raquel Echavarria
Ischemia-reperfusion (I/R) injury, an inevitable result of kidney transplantation, triggers early inflammatory events that affect graft viability. Evidence from human transplantation and preclinical models of I/R suggests that a female hormonal environment positively influences the ability to recover from ischemic injury. However, the mechanisms behind these effects remain mostly unexplored. Here,
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C-peptide and islet transplantation improve glomerular filtration barrier in diabetic nephropathy rats. Transpl. Immunol. (IF 1.624) Pub Date : 2020-08-13 Xiaojie Ni,Ziqiang Xu,Jinjun Wang,Shaoling Zheng,Yong Cai
Objectives Islet transplantation has been proved to be effective in delaying early stage of DN. This study was established to observe the mechanism of islet transplantation on early diabetic nephropathy (DN). Method The diabetes mellitus (DM) rat model was established by an injection of a single-dose streptozotocin. According to the treatment, the rats were randomly divided into 4 groups: the untreated
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Mathematical modeling of peripheral blood neutrophil kinetics to predict CLAD after lung transplantation. Transpl. Immunol. (IF 1.624) Pub Date : 2020-07-23 Benjamin Coiffard,Martine Reynaud-Gaubert,Jean-Baptiste Rey,Elissa Cousin,Charlotte Grosdidier,Corinne Nicolino-Brunet,Françoise Dignat-George,Laurent Papazian,Pascal Alexandre Thomas,Dominique Barbolosi,Raphaël Serre
Background The presence of neutrophils in the lung was identified as a factor associated with CLAD but requires invasive samples. The aim of this study was to assess the kinetics of peripheral blood neutrophils after lung transplantation as early predictor of CLAD. Methods We retrospectively included all recipients transplanted in our center between 2009 and 2014. Kinetics of blood neutrophils were
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Toll-like 4 receptor (TLR4) expression on peripheral blood mononuclear cells in renal transplant recipients with pre-transplant chronic interstitial nephritis indicates patients at risk of graft deterioration. Transpl. Immunol. (IF 1.624) Pub Date : 2020-07-18 Sławomir C Zmonarski,Miroslaw Banasik,Tomasz Golebiowski,Katarzyna Madziarska,Oktawia Mazanowska,Marta Myszka,Joanna Zmonarska,Krzysztof Letachowicz,Tomasz Dawiskiba,Magdalena Krajewska
Data binding the expression of Toll-like 4 receptor (TLR4ex), transplanted kidney function, and the cause of pre-transplant end-stage renal disease are scarcely available. Objective To investigate the relationship between pre-transplant chronic interstitial nephritis (CIN), TLR4ex and transplanted kidney function. Materials and methods TLR4ex was measured in peripheral blood mononuclear cells of 43
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MiR-669b-3p regulates CD4+ T cell function by down-regulating indoleamine-2, 3-dioxygenase. Transpl. Immunol. (IF 1.624) Pub Date : 2020-07-17 Chuan Li,Xi Wang,Fang Yuan,Zhicheng Zhao,Baotong Zhang,Jiehong Zhang,WeiDong Li,Tong Liu
Objective Acute rejection is a major cause of morbidity and mortality after solid organ transplantation. Therefore, optimizing treatment strategies and improving curative effect is urgent and necessary. Reliable biomarkers for acute rejection and the underlying molecular mechanisms remain to be determined. Methods In this study, we established a mouse-to-mouse cardiac transplantation model and identified
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Improving HLA matching in living donor kidney transplantation using kidney paired exchange program. Transpl. Immunol. (IF 1.624) Pub Date : 2020-07-04 Sandra Tafulo,Jorge Malheiro,Leonídio Dias,Luísa Lobato,Luís Ramalhete,António Martinho,Catarina Bolotinha,Rita Costa,Margarida Ivo
Background The inclusion of compatible pairs within kidney paired exchange programs has been described as a way to enhance these programs. Improved immunological matching for the recipient in compatible pair has been described to be a possible benefit. Methods The main purpose of our study was to determine if the introduction of compatible pairs in the Portuguese kidney paired exchange program would
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Impact of HLA polymorphisms among cadaveric donors on kidney graft allocation. Transpl. Immunol. (IF 1.624) Pub Date : 2020-07-03 Zorana Grubic,Marija Burek Kamenaric,Julijana Kljajic,Katarina Stingl Jankovic,Marija Maskalan,Renata Zunec
This study provides data on HLA-A, -B, and -DRB1 frequencies among 861 end-stage renal disease (ESRD) patients from Croatia and estimates the benefit of the kidney exchange program by comparing HLA distribution and assessing HLA mismatches (MMs) within a group of ESRD patients who received kidney grafts from 707 cadaveric donors (422 from Croatia and 285 from Eurotransplant). Patients positive for
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Investigating complement mediated interference in class I HLA-specific antibodies following renal transplantation. Transpl. Immunol. (IF 1.624) Pub Date : 2020-06-20 Petra Goldsmith,David Lowe,Chang Wong,Matthew Howse,Dan Ridgway,Abdul Hammad,Sanjay Mehra,Steve Christmas,Howard Gebel,Robert Bray,Andrew Jones
Introduction Single antigen bead testing (SAB) for HLA-specific antibody enables efficient organ allocation and aids in the diagnosis of antibody mediated rejection. In this retrospective cohort study, a population of kidney transplant recipients possessing HLA Class I antibodies was used to evaluate the best method for resolving complement interference, the so called “prozone” effect. The aim was
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Guy Alexandre - An appreciation of his innovative contributions to organ transplantation. Transpl. Immunol. (IF 1.624) Pub Date : 2020-06-15 David K C Cooper
Guy Alexandre made major contributions to organ transplantation that, in my opinion, have not been sufficiently recognized by the transplant community. To make his contributions better known, I present a brief summary of the innovations for which he was largely responsible. As a research fellow at the Peter Bent Brigham Hospital in Boston in 1962–3, under subsequent Nobelist Joseph Murray, he contributed
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Diagnosis and treatment of acute graft-versus-host disease after liver transplantation: A report of 11cases. Transpl. Immunol. (IF 1.624) Pub Date : 2020-06-12 Xiao-Fei Zhao,Dong-Dong Lin,Ning Li,Ju-Shan Wu,Qing-Liang Guo,Lu Wang
Objective: This study aims to summarize a clinical experience on the diagnosis and treatment of acute graft-versus-host disease (aGVHD) after liver transplantation. Methods: Between April 2005 and August 2016, 11 recipients who underwent OLT developed aGVHD with clinical symptoms of fever, rash, diarrhea and pancytopenia. T lymphocyte chimerism was detected though STR-PCR. These patients were treated
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Limited efficacy of rapamycin monotherapy in vascularized composite allotransplantation. Transpl. Immunol. (IF 1.624) Pub Date : 2020-06-11 Heng Xu,Zvi Steinberger,Liqing Wang,Rongxiang Han,Yixin Zhang,Wayne W Hancock,L Scott Levin
Background Vascularized composite allotransplantation (VCA) is a novel and life-enhancing procedure to restore a patient's function and/or appearance. Current immunosuppression in VCA recipients is based on calcineurin inhibitor (CNI) therapy that can lead to severe complications, such that inducing immune tolerance is a major goal of VCA research. In contrast to CNI, rapamycin (RPM) is thought to
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Predictive value of HLAMatchmaker and PIRCHE-II scores for de novo donor-specific antibody formation after adult and pediatric liver transplantation. Transpl. Immunol. (IF 1.624) Pub Date : 2020-05-16 Sarah Hamada,Jérôme Dumortier,Céline Thévenin,Georges-Philippe Pageaux,Stéphanie Faure,Olivier Guillaud,Olivier Boillot,Alain Lachaux,Dan-Adrian Luscalov,Valérie Dubois,Magdalena Meszaros
Production of de novo DSA (dnDSA) is associated with an increased risk of antibody mediated rejection after liver transplantation. Antibodies not only recognize the entire antigen but are able to bind specific functional epitopes present on the HLA molecule surface. The HLAMatchmaker and the PIRCHE-II (predicted indirectly recognizable HLA epitopes) algorithms are able to determine predictive epitope
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The NLRP3 inhibitor Mcc950 attenuates acute allograft damage in rat kidney transplants. Transpl. Immunol. (IF 1.624) Pub Date : 2020-05-11 Xun-Feng Zou,Jian-Hua Gu,Ji-Hui Duan,Zhan-Dong Hu,Zi-Lin Cui
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Outcome of donor lymphocyte infusion for fall in chimerism after hematopoietic stem cell transplant. Transpl. Immunol. (IF 1.624) Pub Date : 2020-05-07 Prashant Pandey,Divya Setya,Esha Kaul,Shweta Ranjan,Praveen Kumar
Achievement of complete donor chimerism after an allogeneic hematopoietic stem cell transplant is necessary for elimination of underlying malignant disease. A decline in donor chimerism may herald an impending relapse and therefore, early recognition and intervention plays an important role in such cases. A 32 year old male patient diagnosed as a case of Philadelphia positive mixed phenotypic acute
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Flow cytometry crossmatching to investigate kidney-biopsy-proven, antibody-mediated rejection in patients who develop de novo donor-specific antibodies. Transpl. Immunol. (IF 1.624) Pub Date : 2020-05-05 Cédric Usureau,Valentine Jacob,Valentin Clichet,Claire Presne,Judith Desoutter,Coralie Poulain,Gabriel Choukroun,Nicolas Guillaume
Introduction The appearance of de novo donor-specific anti-human leukocyte antigen antibodies (dnDSAs) after kidney transplantation is independently associated with poor long-term allograft outcomes. The objective of the present study was to evaluate the predictive value of a flow cytometry crossmatching (FC-XM) assay after the appearance of dnDSAs related to antibody-mediated allograft rejection (ABMR)
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Immediate impact of COVID-19 on transplant activity in the Netherlands. Transpl. Immunol. (IF 1.624) Pub Date : 2020-05-01 A P J de Vries,I P J Alwayn,R A S Hoek,A P van den Berg,F C W Ultee,S M Vogelaar,B J J M Haase-Kromwijk,M B A Heemskerk,A C Hemke,W N Nijboer,B S Schaefer,M A Kuiper,J de Jonge,N P van der Kaaij,M E J Reinders
The rapid emergence of the COVID-19 pandemic is unprecedented and poses an unparalleled obstacle in the sixty-five year history of organ transplantation. Worldwide, the delivery of transplant care is severely challenged by matters concerning - but not limited to - organ procurement, risk of SARS-CoV-2 transmission, screening strategies of donors and recipients, decisions to postpone or proceed with
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Differential effects of Belatacept on virus-specific memory versus de novo allo-specific T cell responses of kidney transplant recipients and healthy donors. Transpl. Immunol. (IF 1.624) Pub Date : 2020-04-21 Jenny Franziska Kühne,Christine Neudörfl,Kerstin Beushausen,Jana Keil,Svitlana Malysheva,Franziska Wandrer,Hermann Haller,Martin Messerle,Cornelia Blume,Michael Neuenhahn,Fabian Schlott,Wolfgang Hammerschmidt,Reinhard Zeidler,Christine S Falk
Belatacept, Nulojix®, inhibits the interaction of CD28 on naïve T cells with B7.1/B7.2 (CD80/86) on antigen presenting cells, leading to T cell hyporesponsiveness and anergy and is approved as immunosuppressive drug in kidney transplantation. Due to its specificity for B7.1/2 molecules, side effects are reduced compared to other immunosuppressive drugs like calcineurin- and mTOR-inhibitors. Kidney
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The implication of genetic variation in the complement C3 allotypes on the first-year allograft outcome after live donor liver transplantation. Transpl. Immunol. (IF 1.624) Pub Date : 2020-04-17 Samah Mohamed Awad,Mohammed Taha,Mahmoud Omar,Ashraf Khalil
Background The component (C3) of the complement system constitutes a central element in liver transplantation. C3 is produced mainly by the liver and comprises both slow (C3-S), and fast (C3-F) components. Methods The effect of a single nucleotide variation in the C3 gene on the first-year outcome examined by ARMS PCR in 30 recipients of living donor allograft. Results Frequencies of C3-S and C3-F
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Gastric submucosal alleviated pro-inflammation cytokines mediated initial dysfunction of islets allografts. Transpl. Immunol. (IF 1.624) Pub Date : 2020-04-14 Zhuzeng Yin,Jiaxin Li,Yang Zheng,Shusen Wang,Ximo Wang
BACKGROUND The liver and renal capsule are the most common site for experimental pancreatic islet transplantation, but it is not optimal. Gastric submucosa space may be an ideal site for islet transplantation; however, whether pro-inflammation factors mediated islet dysfunction could be avoided or alleviated is still unclear. METHODS Islets of Sprague Dawley (SD) rat were transplanted into the streptozotocin-induced
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High expression of granzyme B in conventional CD4+ T cells is associated with increased relapses after allogeneic stem cells transplantation in patients with hematological malignancies. Transpl. Immunol. (IF 1.624) Pub Date : 2020-04-14 Mikhail Drokov,Yulia Davydova,Natalia Popova,Nikolay Kapranov,Olga Starikova,Ekaterina Mikhaltsova,Maria Nareyko,Anna Dmitrova,Zoya Konova,Irina Galtseva,Larisa Kuzmina,Elena Parovichnikova,Valery Savchenko
Granzyme B is known to be a serine protease contained in granules of cytotoxic T cells. We have previously reported an influence of granzyme B expression in T regulatory cells (Tregs) on the risk of acute graft versus host disease (GVHD) onset. However, it is still unknown if conventional T cells (Tcon) use the granzyme B pathway as a mechanism of alloimmunity. We hypothesized that granzyme B in Tcon
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Reduced CD4+ CD25++ CD45RA- Foxp3hi activated regulatory T cells and its association with acute rejection in patients with kidney transplantation. Transpl. Immunol. (IF 1.624) Pub Date : 2020-03-30 Mohammad Mirzakhani,Mehdi Shahbazi,Roghayeh Akbari,Farshid Oliaei,Masoumeh Asgharpour,Hassan Nikoueinejad,Mousa Mohammadnia-Afrouzi
Background It was found that regulatory T cells (Tregs) importantly affect the maintenance of the kidney graft. However, Tregs are a heterogeneous population with less to more suppressive activity. The aim of this study was to determine the effects of different subsets of Tregs, as well as their ratio to effector T cells (Teff), on kidney transplantation outcomes. Methods A total of 58 participants
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Dialysis therapy is associated with peripheral marginal zone B-cell augmentation. Transpl. Immunol. (IF 1.624) Pub Date : 2020-03-27 Lucia Stranavova,Petra Hruba,Janka Slatinska,Birgit Sawitzki,Petra Reinke,Hans-Dieter Volk,Ondrej Viklicky
Chronic kidney disease stage 5 (CKD5) dialysis patients who stay long term in uremic environment often exhibit several, poorly defined, immune impairments. In this study, we assessed peripheral virus-specific effector/memory cells and subpopulations of T, B and DC cells using ELISPOT and FACS methods in 74 low-risk kidney transplant candidates without anti-HLA antibodies, prior to transplantation in
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Acute cellular rejection in small-bowel transplantation impairs NCR+ innate lymphoid cell subpopulation 3/interleukin 22 axis. Transpl. Immunol. (IF 1.624) Pub Date : 2020-03-21 Melisa Pucci Molineris,Virginia González Polo,Carolina Rumbo,Claudia Fuxman,Carlos Lowestein,Fabio Nachman,Martín Rumbo,Gabriel Gondolesi,Dominik Meier
Acute cellular rejection (ACR) remains as one of the main causes of graft loss and death in intestinal transplant (ITx) patients. ACR promotes intestinal injury, disruption of the mucosal barrier, bacterial translocation, and organ dysfunction. As epithelial regeneration is critical in reversing these consequences, the functional axis between the innate lymphoid cell subpopulation 3 (ILC3) and interleukin
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The role of HLA-DP mismatches and donor specific HLA-DP antibodies in kidney transplantation: a case series. Transpl. Immunol. (IF 1.624) Pub Date : 2020-03-16 Liesbeth Daniëls,Frans H J Claas,Cynthia S M Kramer,Aleksandar Senev,Marleen Vanden Driessche,Marie-Paule Emonds,Steven Van Laecke,Rachel Hellemans,Daniel Abramowicz,Maarten Naesens
BACKGROUND The impact of HLA-DP mismatches on renal allograft outcome is still poorly understood and is suggested to be less than that of the other HLA loci. The common association of HLA-DP donor-specific antibodies (DSA) with other DSA obviates the evaluation of the actual effect of HLA-DP DSA. METHODS From a large multicenter data collection, we retrospectively evaluated the significance of HLA-DP
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A potential role of neutrophil extracellular traps (NETs) in kidney acute antibody mediated rejection. Transpl. Immunol. (IF 1.624) Pub Date : 2020-03-07 Jiram Torres-Ruiz,Roxana Villca-Gonzales,Diana Gómez-Martín,Alejandro Zentella-Dehesa,Miguel Tapia-Rodríguez,Norma O Uribe-Uribe,Luis E Morales-Buenrostro,Josefina Alberú
Background The aim of this study was to evaluate neutrophil extracellular traps (NETs) in kidney transplant recipients (KTR) and their potential involvement in acute antibody-mediated rejection (AAMR). Methods We studied 3 groups: KTR with AAMR (KTR-Cases, n = 14); KTR without any immunologic event (KTR-Controls, n = 14) and donors (n = 12). Spontaneous and lipopolysaccharide-induced NETosis were evaluated
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Serum dilutions as a predictive biomarker for peri-operative desensitization: An exploratory approach to transplanting sensitized heart candidates. Transpl. Immunol. (IF 1.624) Pub Date : 2020-03-04 Olga A Timofeeva,Rene Alvarez,Justin Pelberg,Edward Yoon,Mohamed Alsammak,Steve S Geier,Christina Ruggia-Check,Jared Hassler,Jamael Hoosain,Meredith A Brisco,Nana Afari-Armah,Val Rakita,Stacey Brann,Suresh Keshavamurthy,Jesus Gomez-Abraham,Kenji Minakata,Yoshiya Toyoda,Eman Hamad
Antibody-mediated rejection (AMR) of cardiac allografts mediated by anti-HLA Donor Specific Antibodies (DSA) is one of the major barriers to successful transplantation for the treatment of end-stage heart failure. Therapeutic plasma exchange (TPE) is a first-line treatment for pre-transplant desensitization. However, indications for treatment regimens and treatment end-points have not been well established
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Epithelial-mesenchymal transition and membrane microparticles: Potential implications for bronchiolitis obliterans syndrome after lung transplantation. Transpl. Immunol. (IF 1.624) Pub Date : 2020-02-22 Benjamin Renaud-Picard,Kevin Vallière,Justine Toussaint,Guillaume Kreutter,Ali El-Habhab,Mohamad Kassem,Fatiha El-Ghazouani,Anne Olland,Sandrine Hirschi,Michele Porzio,Marie-Pierrette Chenard,Florence Toti,Laurence Kessler,Romain Kessler
Long term survival post lung transplantation (LTx) is limited by the occurrence of bronchiolitis obliterans syndrome (BOS). One mechanism involved is the epithelial-mesenchymal transition (EMT). Membrane microparticles (MPs) are known to be involved in some respiratory diseases and in other organs allograft rejection episodes. We hypothesized that leukocyte-derived MPs likely contribute to EMT. To
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Exploring predicted indirectly recognizable HLA epitopes (PIRCHE-II) in liver transplant recipients on calcineurin inhibitor-free maintenance immunosuppression. A retrospective single center study. Transpl. Immunol. (IF 1.624) Pub Date : 2020-02-12 Magdalena Meszaros,Matthias Niemann,José Ursic-Bedoya,Stéphanie Faure,Lucy Meunier,Benjamin Rivière,Valérie Costes-Martineau,Céline Thevenin,Georges-Philippe Pageaux
The PIRCHE (Predicted Indirectly ReCognizable HLA Epitopes) score is an HLA epitope matching algorithm. PIRCHE algorithm estimates the level of presence of T-cell epitopes in mismatched HLA. The PIRCHE-II numbers associate with de novo donor-specific antibody (dnDSA) formation following liver transplantation and kidney allograft survival following renal transplantation. The aim of our study was to
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Pre-existing self-reactive IgA antibodies associated with primary graft dysfunction after lung transplantation. Transpl. Immunol. (IF 1.624) Pub Date : 2020-01-30 Vaidehi Kaza,Chengsong Zhu,Leying Feng,Fernando Torres,Srinivas Bollineni,Manish Mohanka,Amit Banga,John Joerns,T Mohanakumar,Lance S Terada,Quan-Zhen Li
Background Primary graft Dysfunction (PGD) results in significant mortality and morbidity after lung transplantation (LT). The objective of this study was to evaluate if pre-existing antibodies to self-antigens in sera of LT recipients are associated with PGD. Methods The serum profiles of IgG and IgA autoantibodies were analyzed using a customized proteomic microarray bearing 124 autoantigens. Autoantibodies
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Combined treatment with vitamin D3 and antibody agents suppresses secondary heart transplant rejection in the early postoperative period. Transpl. Immunol. (IF 1.624) Pub Date : 2020-01-15 Baiyi Xie,Yunhan Ma,Yanfeng Xi,Anjie Di,Xu Chen,Yingyu Chen,Liyi Zhang,Shuangyue Xu,Chenxi Wang,Guoliang Yan,Zhongquan Qi
Background Accelerated transplant rejection mediated by donor reactive memory T cells is another barrier to the induction of graft tolerance. The aim of this study was to investigate the immunosuppressing effects of vitamin D (1,25(OH)2D3), administered alone or in combination with a costimulatory blockade treatment, on rejection of secondary heart allografts in a mouse model. Methods Circular full-thickness
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Receptor activator of the NFκB ligand system protects renal function during experimental renal ischemia-reperfusion in mice. Transpl. Immunol. (IF 1.624) Pub Date : 2020-01-03 Yuki Nakayama,Naohito Isoyama,Takeshi Yamamoto,Yudai Nagata,Masafumi Matsumura,Koki Fujikawa,Hideyasu Matsuyama,Koji Shiraishi
Renal ischemia-reperfusion (I/R) injury is closely associated with delayed graft function and poor long-term graft survival following transplantation. Various pathophysiologies can cause the deterioration of renal function; however, the immune system plays important roles in promoting and protecting renal tissues. Receptor activator of NFκB ligand (RANKL) is a member of the TNF superfamily and is produced
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Prospective validation of the L-index reflecting both the intensity and duration of lymphopenia and its detailed evaluation using a lymphocyte subset analysis after allogeneic hematopoietic stem cell transplantation. Transpl. Immunol. (IF 1.624) Pub Date : 2019-12-30 Shun-Ichi Kimura,Miki Sato,Yukiko Misaki,Kazuki Yoshimura,Ayumi Gomyo,Jin Hayakawa,Yu Akahoshi,Naonori Harada,Masaharu Tamaki,Machiko Kusuda,Kazuaki Kameda,Hidenori Wada,Koji Kawamura,Kiriko Terasako-Saito,Misato Kikuchi,Aki Tanihara,Hideki Nakasone,Shinichi Kako,Yoshinobu Kanda
We prospectively validated the previously reported L-index, which reflects both the intensity and duration of lymphopenia, and further evaluated it using a lymphocyte subset analysis after allogeneic hematopoietic stem cell transplantation (HSCT) (n = 30). The L-index was defined as the area over the lymphocyte curve during lymphopenia (<700/μl), and calculated from the start of conditioning to day30
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An in vitro model of antibody-mediated injury to glomerular endothelial cells: Upregulation of MHC class II and adhesion molecules. Transpl. Immunol. (IF 1.624) Pub Date : 2019-12-27 Nancy A Wilson,James Dylewski,Kenna R Degner,Megan A O'Neill,Shannon R Reese,Luis G Hidalgo,Judith Blaine,Sarah E Panzer
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