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CRISPR activation as a platform to identify interferon stimulated genes with anti-viral function Innate Immun. (IF 3.2) Pub Date : 2024-01-23 Emily N. Kirby, Xavier B. Montin, Timothy P. Allen, Jaslan Densumite, Brooke N. Trowbridge, Michael R. Beard
Interferon Stimulated Gene (ISG) expression plays a key role in the control of viral replication and development of a robust adaptive response. Understanding this dynamic relationship between the pathogen and host is critical to our understanding of viral life-cycles and development of potential novel anti-viral strategies. Traditionally, plasmid based exogenous prompter driven expression of ISGs has
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Immunomodulatory actions of myeloid-derived suppressor cells in the context of innate immunity. Innate Immun. (IF 3.2) Pub Date : 2023-11-28 Nikoleta Bizymi,Andreas M Matthaiou,Irene Mavroudi,Aristea Batsali,Helen A Papadaki
Myeloid-derived suppressor cells (MDSCs) are notable innate immune cells, which are further divided into two subpopulations, i.e., monocytic and granulocytic. These cells are traditionally considered to mainly suppress the T-cell responses. However, more updated data indicate that their properties are rather immunomodulatory than solely immunosuppressive. Indeed, MDSCs display extensive crosstalk with
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Production of a p65fl/fl/LysMCre mouse model with dysfunctional NF-κB signaling in bone marrow-derived macrophages. Innate Immun. (IF 3.2) Pub Date : 2023-10-13 Ahmet K Korkaya,Jeffrey Fischer,Anthony Peppers,Sean M Crosson,Manira Rayamajhi,Edward A Miao,Albert S Baldwin,Jennifer W Bradford
Here, we describe the production and characterization of a novel p65fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite
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Obesity Alters cytokine signaling and gut microbiome in septic mice. Innate Immun. (IF 3.2) Pub Date : 2023-10-06 Lauren Bodilly,Lauren Williamson,Patrick Lahni,Matthew N Alder,David B Haslam,Jennifer M Kaplan
Sepsis is a leading cause of mortality. Plasma cytokine levels may identify those at increased risk of mortality from sepsis. Our aim was to understand how obesity alters cytokine levels during early sepsis and its correlation with survival. Six-week-old C57BL/6 male mice were randomized to control (non-obese) or high fat diet (obese) for 5-7 weeks. Sepsis was induced by cecal ligation and perforation
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Initial exploration of a novel fusion protein, IL-4/IL-34/IL-10, which promotes cardiac allograft survival mice through alloregulation. Innate Immun. (IF 3.2) Pub Date : 2023-10-01 Young S Lee,I-Ting Cheng,Godoy-Ruiz Raquel,David J Weber,Joseph R Scalea
Immune mediated graft loss still represents a major risk to transplant recipients. Creative approaches to immunosuppression that exploit the recipient's own alloregulatory mechanisms could reduce the need for pharmacologic immunosuppression and potentially induce immune tolerance. In the process of studying recipient derived myeloid derived suppressor cells (MDSCs), we identified key alloregulatory
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Potential effects of gingerol topical application on components of the innate immunity in lactating goat mammary glands. Innate Immun. (IF 3.2) Pub Date : 2023-08-22 Yusaku Tsugami,Takahiro Nii,Ken Kobayashi,Naoki Isobe
In the mammary glands, production of antimicrobial components and formation of less-permeable tight junctions (TJs) are important for safe milk production. Previously, we reported that local heat treatment of udders using disposable heating pad enhances the components of innate immunity in lactating goat mammary glands. Gingerol is a polyphenol present in ginger that can induce heat-like effects. However
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Dietary fat differentially modulates the response of bone marrow-derived macrophages to TLR4 and NOD2 agonists. Innate Immun. (IF 3.2) Pub Date : 2023-08-07 Michael G Shehat,Madelyn H Miller,Ashley N Calder,Timothy A Gilbertson,Justine T Tigno-Aranjuez
Consumption of diets high in fat has been linked to the development of obesity and related metabolic complications. Such associations originate from the enhanced, chronic, low-grade inflammation mediated by macrophages in response to translocated bacteria, bacterial products, or dietary constituents such as fatty acids (FAs). Nucleotide-binding Oligomerization Domain 2 (NOD2) senses muramyl dipeptide
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Role of neutrophils in different stages of atherosclerosis. Innate Immun. (IF 3.2) Pub Date : 2023-07-25 Xiaojing Zhang,Zhanfang Kang,Dazhong Yin,Jun Gao
Neutrophils constitute the first line of defense in human immunity and can be attracted to inflamed and infected sites by various chemokines. As essential players in immune processes, neutrophils theoretically play integral roles in the course of chronic inflammation-induced atherosclerosis. However, because neutrophils are rarely found in atherosclerotic lesions, their involvement in the pathophysiological
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Killer cell immunoglobulin-like receptor (KIR) genes and their HLA ligands in a Brazilian population. Innate Immun. (IF 3.2) Pub Date : 2023-06-22 Daniela Maira Cardozo,Amanda Vansan Marangon,Fernando Guimarães,Silvia Marques,Sofia Lieber,Márcia Delamain,Francisco Aranha,Jeane Eliete Laguila Visentainer,Cármino Antonio de Souza
Killer cell immunoglobulin-like receptors (KIR) exhibit extensive diversity, giving rise to different KIR profiles in populations worldwide. This study aimed to investigate the distribution of KIR genes and HLA ligands in a population from Campinas, southeastern Brazil (n = 292), and to compare their results with other populations. A comprehensive analysis of population-specific genes, genotype,
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Association between interleukin-27 gene polymorphisms and Plasmodium falciparum Malaria. Innate Immun. (IF 3.2) Pub Date : 2023-06-12 Nada H Aljarba,Mashael R Al-Anazi,Tahani M Al-Hazani,Mohammed I Shafeai,Fuad H Rudiny,Ali M Motaen,Saad M Bin Dajem,Hani Alothaid,Jahad Alghamdi,Saad Alkahtani,Ahmed A Al-Qahtani
Malaria is often characterized by a complicated disease course due to multifaceted intrinsic genetic factors of the host and the parasite. This study aimed to investigate the role of interleukin-27 (IL-27) gene polymorphisms in Plasmodium falciparum malaria infection in a Saudi Arabian cohort. This case-control study obtained blood samples from 250 malaria patients with P. falciparum and 200 randomly
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Single cell RNA-sequencing of human precision-cut lung slices: A novel approach to study the effect of vaping and viral infection on lung health. Innate Immun. (IF 3.2) Pub Date : 2023-06-12 Taylor Crue,Grace Yihua Lee,Joyce Yao-Chun Peng,Niccolette Schaunaman,Hina Agraval,Brian J Day,Kris Genelyn Dimasuay,Diana Cervantes,Hamid Nouri,Taylor Nichols,Paige Hartsoe,Mari Numata,Irina Petrache,Hong Wei Chu
Vaping is an increasing health threat in the US and worldwide. The damaging impact of vaping on the human distal lung has been highlighted by the recent epidemic of electronic cigarette or vaping use-associated lung injury (EVALI). The pathogenesis of EVALI remains incompletely understood, due to a paucity of models that recapitulate the structural and functional complexity of the human distal lung
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Recognition of Listeria monocytogenes infection by natural killer cells: Towards a complete picture by experimental studies in rats. Innate Immun. (IF 3.2) Pub Date : 2023-06-07 Hamid Shegarfi
The study of cellular immune responses in animal disease models demands detailed knowledge of development, function, and regulation of immune cells, including natural killer (NK) cells. Listeria monocytogenes (LM) bacterium has been explored in a large area of research fields, including the host pathogen interaction. Although the importance role of NK cells in controlling the first phase of LM burden
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Increased circulating monocyte MDSCs positively correlate with serum Interleukin-10 in metastatic melanoma patients Innate Immun. (IF 3.2) Pub Date : 2023-05-09 Katarina Mirjačić Martinović, Ana Vuletić, Nevena Tišma Miletić, Milica Nedeljković, Nada Babović, Suzana Matković, Vladimir Jurišić
Numerous immunosuppressive cells such as myeloid-derived suppressor cells (MDSCs) and inhibitory cytokines identified in melanoma microenvironment have the important role in immune escape. Therefor...
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C/EBPβ deficiency enhances the keratinocyte innate immune response to direct activators of cytosolic pattern recognition receptors Innate Immun. (IF 3.2) Pub Date : 2023-04-24 John S. House, Sophia Gray, Jennifer R. Owen, Dereje D. Jima, Robert C. Smart, Jonathan R. Hall
The skin is the first line of defense to cutaneous microbes and viruses, and epidermal keratinocytes play a critical role in preventing infection by viruses and pathogens through activation of the ...
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A TLR4 agonist liposome formulation effectively stimulates innate immunity and enhances protection from bacterial infection Innate Immun. (IF 3.2) Pub Date : 2023-04-21 Jodi F. Hedges, Deann T. Snyder, Amanda Robison, Macy A. Thompson, Klara Aspelin, Jack Plewa, Jory Baldridge, Mark A. Jutila
Stimulation of innate immunity can protect against infectious insult and could be used in combination with other therapies. Since antibiotic resistance is an increasing concern, strategies to reduc...
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SENP3 facilitates M1 macrophage polarization via the HIF-1α/PKM2 axis in lipopolysaccharide-induced acute lung injury Innate Immun. (IF 3.2) Pub Date : 2023-04-05 Shuangjun He, Chenyu Fan, Yiming Ji, Qian Su, Feng Zhao, Cuiying Xie, Xuelian Chen, Yang Zhang, Yi Chen
M1/M2 macrophage polarization plays a pivotal role in the development of acute lung injury (ALI). The hypoxia-inducible factor-1α/pyruvate kinase M2 (HIF-1α/PKM2) axis, which functions upstream of ...
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Pyroptosis, inflammasome, and gasdermins in tumor immunity Innate Immun. (IF 3.2) Pub Date : 2023-01-11 Xiaohu Ouyang, Junfen Zhou, Lan Lin, Zili Zhang, Shanshan Luo, Desheng Hu
The gasdermins (GSDM), a family of pore-forming proteins, consist of gasdermin A (GSDMA), gasdermin B (GSDMB), gasdermin C (GSDMC), gasdermin D (GSDMD), gasdermin E (GSDME) and DFNB59 (Pejvakin (PJ...
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Knockdown of DAPK1 inhibits IL-1β-induced inflammation and cartilage degradation in human chondrocytes by modulating the PEDF-mediated NF-kB and NLRP3 inflammasome pathway Innate Immun. (IF 3.2) Pub Date : 2022-11-21 Zhongyuan Zhao, Wei Liu, Gong Cheng, Shengjie Dong, Yuchi Zhao, Hao Wu, Zhilin Cao
Osteoarthritis (OA) is a common joint disease that is characterized by inflammation and cartilage degradation. Death-associated protein kinase 1 (DAPK1) is a multi-domain serine/threonine kinase an...
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Intravenous anti-P. aeruginosa IgY-antibodies do not decrease pulmonary bacterial concentrations in a porcine model of ventilator-associated pneumonia Innate Immun. (IF 3.2) Pub Date : 2022-11-13 A. Otterbeck, P. Skorup, K. Hanslin, A. Larsson, J. Stålberg, H. Hjelmqvist, M. Lipcsey
Ventilator associated pneumonia (VAP) caused by P. aeruginosa is a cause of morbidity and mortality in critically ill patients. The spread of pathogens with anti-microbial resistance mandates the i...
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Surfactant proteins and innate immunity of otitis media Innate Immun. (IF 3.2) Pub Date : 2022-09-07 Osama Abdel-Razek, Jason Audlin, Dennis S. Poe, Guirong Wang
Otitis media (OM) is the most common disease among young children and one of the most frequent reasons to visit the pediatrician. Development of OM requires nasopharyngeal colonization by a pathoge...
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Obituary Louis Selim Chedid, MD PhD IEIIS honorary life member Innate Immun. (IF 3.2) Pub Date : 2022-08-17 Jean-Marc Cavaillon
Louis Selim Chedid was born in Cairo (Egypt) in June 1922 and died in Paris in March 2021 at the age of 98. After obtaining his bachelor's degree in Cairo, he started his medical studies in Beirut and completed them in Paris, defending his medical thesis on artificial estrogen in 1947. After being trained in Egypt and the United States, he joined the laboratory of Robert Courrier at the College de
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Non-classical monocytes contribute to innate immune training in cattle Innate Immun. (IF 3.2) Pub Date : 2022-07-25 Lisa-Marie Schünemann, Hans-Joachim Schuberth
Innate immune training is defined as a property of innate immune cells to react stronger to a secondary contact with pathogens. Induction of innate immune training has been reported for a variety o...
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Natural killer cell exhaustion in SARS-CoV-2 infection Innate Immun. (IF 3.2) Pub Date : 2022-06-22 Janet Gallardo-Zapata, Carmen Maldonado-Bernal
At the end of 2019, an outbreak of a severe respiratory disease occurred in Wuhan China, and an increase in cases of unknown pneumonia was alerted. In January 2020, a new coronavirus named SARS-CoV...
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Circ_0062491 alleviates LPS-induced apoptosis and inflammation in periodontitis by regulating miR-498/SOCS6 axis Innate Immun. (IF 3.2) Pub Date : 2022-06-09 Lie Wang, Yanli Li, Feifei Hong, Haiyan Ning
Periodontitis is a prevalent chronic inflammatory disease. Circular RNAs (circRNAs) have been revealed to play roles in the inflammatory response. Hence, this work aimed to explore the role and mec...
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Circ_0066881 targets miR-144-5p/RORA axis to alleviate LPS-induced apoptotic and inflammatory damages in human periodontal ligament cells Innate Immun. (IF 3.2) Pub Date : 2022-05-29 Qin Li, Zhaopeng Hu, Fang Yang, Yi Peng
Circular RNAs (circRNAs) are involved in the regulation of various diseases, including periodontitis. The objective of this study was to analyze the biological role and regulatory mechanism of circ...
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Differential susceptibility to lipopolysaccharide affects the activation of toll-like-receptor 4 signaling in THP-1 cells and PMA-differentiated THP-1 cells Innate Immun. (IF 3.2) Pub Date : 2022-05-25 Young Kyu Kim, Jeong Ho Hwang, Hoon Taek Lee
Monocytes and macrophages that originate from common myeloid progenitors perform various crucial roles in the innate immune system. Stimulation with LPS combined with TLR4 drives the production of ...
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Ursolic acid inhibits Th17 cell differentiation via STAT3/RORγt pathway and suppresses Schwann cell-mediated Th17 cell migration by reducing CXCL9/10 expression Innate Immun. (IF 3.2) Pub Date : 2022-05-12 Hua Xu, Ai-ling Yu, Da-peng Zhao, Guang-yuan Meng, Ling Wang, Min Shan, Nai-xia Hu, Yun-lin Liu
Th17 cells represent important immune cells. Ursolic acid (UA) can regulate immune cell activities. This study was aimed to explore the effects of UA on Th17 cell differentiation and Schwann cell(S...
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The monocyte activation test detects potentiated cytokine release resulting from the synergistic effect of endotoxin and non-endotoxin pyrogens Innate Immun. (IF 3.2) Pub Date : 2022-05-02 Shabnam Solati, Ting Zhang, Shahrzad Timman
Pyrogens are classified in two groups, endotoxin pyrogens and non-endotoxin pyrogens (NEPs). The presence of either in parenteral pharmaceuticals or medical devices can cause severe harm to subjects, and when occurring in combination, synergistic potentiation effects can occur. As the standard in vitro pyrogen test, the Limulus Amebocyte Lysate (LAL) assay can detect LPS only, an endotoxin, but not
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Soluble CD163 is produced by monocyte-derived and alveolar macrophages, and is not associated with the severity of idiopathic pulmonary fibrosis Innate Immun. (IF 3.2) Pub Date : 2022-05-06 Pierre Chauvin, Claudie Morzadec, Bertrand de Latour, Francisco Llamas-Gutierrez, David Luque-Paz, Stéphane Jouneau, Laurent Vernhet
The soluble form of the membrane hemoglobin scavenger receptor CD163 (sCD163), released by shedding, is a strong marker for macrophage activation. Serum sCD163 levels rise in several acute inflamma...
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From bats to pangolins: new insights into species differences in the structure and function of the immune system Innate Immun. (IF 3.2) Pub Date : 2022-05-04 Patrick J. Haley
Species differences in the structure and function of the immune system of laboratory animals are known to exist and have been reviewed extensively. However, the number and diversity of wild and exo...
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Silencing of circ_0000205 mitigates interleukin-1β-induced apoptosis and extracellular matrix degradation in chondrocytes via targeting miR-766-3p/ADAMTS5 axis Innate Immun. (IF 3.2) Pub Date : 2022-04-28 Guowen Li, Heyuan Luo, Zhiyong Ding, Haofeng Liang, Zhoupeng Lai, Shuzhen Chen, Yuliang Huang
The aim of this study was to explore the role of hsa_circRNA_0000205 (circ_0000205) in chondrocyte injury in osteoarthritis (OA) and the underlying mechanism. Expression of circ_0000205, microRNA (miR)-766-3p and a disintegrin and metalloproteinase with thrombospondin motif (ADAMTS)-5 was detected by quantitative real time (qRT)-polymerase chain reaction (PCR) and Western blot assays. Cell proliferation
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The effect of myeloperoxidase-oxidized LDL on THP-1 macrophage polarization and repolarization Innate Immun. (IF 3.2) Pub Date : 2022-04-11 Samer Bazzi, Christian Frangie, Eliana Azar, Jalil Daher
Macrophages (Mφs) play a crucial role in the development of atherosclerosis by engulfing modified LDL particles and forming foam cells, the hallmark of atherosclerosis. Many studies suggest that myeloperoxidase-oxidized LDL (Mox-LDL) is an important pathophysiological model for LDL modification in vivo. Classically (M1) and alternatively activated (M2) Mφs are both implicated in the process of atherogenesis
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A novel antimicrobial peptide derived from human BPIFA1 protein protects against Candida albicans infection Innate Immun. (IF 3.2) Pub Date : 2022-02-24 Nan Hu, Xi-Ming Mo, Shi-Na Xu, Hao-Neng Tang, Ying-Hui Zhou, Long Li, Hou-De Zhou
Bactericidal/permeability-increasing fold containing family A, member 1 (BPIFA1) is an innate immunity defense protein. Our previous studies proved its antibacterial and antiviral effects, but its role in fungi remains unknown. The study aimed to identify antifungal peptides (AFP) derived from BPIFA1, and three antimicrobial peptides (AMP1–3) were designed. The antifungal effects were proved by growth
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Protective effects of the notoginsenoside R1 on acute lung injury by regulating the miR-128-2-5p/Tollip signaling pathway in rats with severe acute pancreatitis Innate Immun. (IF 3.2) Pub Date : 2022-02-10 Ju He, Ming-wei Liu, Zhi-yi Wang, Rong-jie Shi
Notoginsenoside R1 (NG-R1), the extract and the main ingredient of Panax notoginseng, has anti-inflammatory effects and can be used in treating acute lung injury (ALI). In this study, we explored the pulmonary protective effect and the underlying mechanism of the NG-R1 on rats with ALI induced by severe acute pancreatitis (SAP). MiR-128-2-5p, ERK1, Tollip, HMGB1, TLR4, IκB, and NF-κB mRNA expression
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Circ_0026579 alleviates LPS-induced WI-38 cells inflammation injury in infantile pneumonia Innate Immun. (IF 3.2) Pub Date : 2022-02-10 Yang Yu, Tingting Yang, Zhaozheng Ding, Yuan Cao
Circular RNA (circRNA) represents an important regulator in infantile pneumonia progression. To clarify the role of circ_0026579 in this disease, LPS was used to treat WI-38 cells to mimic inflammation injury. The levels of inflammatory factors were determined by ELISA assay. Cell proliferation and apoptosis were measured by MTT assay, EdU staining and flow cytometry. The protein levels of cyclinD1
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Early kinetics of calprotectin in plasma following inguinal hernia surgery Innate Immun. (IF 3.2) Pub Date : 2022-02-01 Kristina Sejersen, Aleksandra Havelka, Pearl Sanchez Salas, Anders Larsson
Calprotectin is one of the most abundant proteins of neutrophil granulocytes. It is released upon neutrophil activation and is considered a sensitive and clinically useful marker for neutrophil-mediated inflammation, including bacterial infections. However, early kinetics of calprotectin activation following inflammatory activation has hitherto been unknown. The aim of the present study was to determine
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Delayed inflammation decrease is associated with mortality in Tocilizumab-treated critically ill SARS-CoV-2 patients: A retrospective matched-cohort analysis Innate Immun. (IF 3.2) Pub Date : 2022-01-28 Tomas Urbina, Jean-Rémi Lavillegrand, Marc Garnier, Arsene Mekinian, Jerome Pacanowski, Nathalie Mario, Guillaume Dumas, Geoffroy Hariri, Antoine Pilon, Lucie Darrivère, Muriel Fartoukh, Bertrand Guidet, Eric Maury, Judith Leblanc, Yannick Chantran, Olivier Fain, Karine Lacombe, Guillaume Voiriot, Hafid Ait-Oufella
Little is known about the immuno-inflammatory response to Tocilizumab and its association with outcome in critically-ill SARS-CoV2 pneumonia. In this multicenter retrospective cohort of SARS-CoV-2 patients admitted to three intensive care units between March and April 2020, we matched on gender and SAPS II 21 Tocilizumab-treated patients to 42 non-treated patients. Need for mechanical ventilation was
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Revisiting Metchnikoff's work in light of the COVID-19 pandemic Innate Immun. (IF 3.2) Pub Date : 2022-01-18 Jean-Marc Cavaillon, Jack Levin
Revisiting Metchnikoff's work in light of the COVID-19 pandemic illustrates how much this amazing scientist was a polymath, and one could speculate how much he would have been fascinated and most interested in following the course of the pandemic. Since he coined the word “gerontology”, he would have been intrigued by the high mortality among the elderly, and by the concepts of immunosenescence and
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Down-regulation of hsa_circ_0045474 induces macrophage autophagy in tuberculosis via miR-582-5p/TNKS2 axis Innate Immun. (IF 3.2) Pub Date : 2021-12-03 Min Wu, Zhibin Liu, Shaojun Zhang
Macrophage autophagy plays a major role in the control and elimination of invading Mycobacterium tuberculosis. However, the function and mechanism of circRNA on macrophage autophagy in tuberculosis remain unclear. Therefore, this study aimed to explore the role of circRNA underlying macrophage autophagy in tuberculosis. Quantitative real-time polymerase chain reaction was used to detect the expression
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Bergamottin alleviates LPS-induced acute lung injury by inducing SIRT1 and suppressing NF-κB Innate Immun. (IF 3.2) Pub Date : 2021-11-23 Ning An, Tao Yang, Xiao-Xia Zhang, Mei-xia Xu
Acute lung injury (ALI) is associated with a high mortality due to inflammatory cell infiltration and lung edema. The development of ALI commonly involves the activation of NF-κB. Since bergamottin is a natural furanocoumarin showing the ability to inhibit the activation of NF-κB, in this study we aimed to determine the effect of bergamottin on ALI. RAW264.7 mouse macrophages were pre-treated with
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Chrysosplenol D protects mice against LPS-induced acute lung injury by inhibiting oxidative stress, inflammation, and apoptosis via TLR4-MAPKs/NF-κB signaling pathways Innate Immun. (IF 3.2) Pub Date : 2021-11-20 Qinqin Zhang, Aozi Feng, Mengnan Zeng, Beibei Zhang, Jingya Shi, Yaxin Lv, Bing Cao, Chenxin Zhao, Mengya Wang, Yifan Ding, Xiaoke Zheng
This study investigated the effect and mechanism of chrysosplenol D (CD) on LPS-induced acute lung injury in mice. Histological changes in the lungs were measured by hematoxylin-eosin staining. The levels of IL-6, IL-1β, and TNF-α in the bronchoalveolar lavage fluid were detected by ELISA. The levels of oxidative stress were detected by the cuvette assay. Immune cells in peripheral blood, the levels
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TLRs in COVID-19: How they drive immunopathology and the rationale for modulation Innate Immun. (IF 3.2) Pub Date : 2021-11-20 F. Linzee Mabrey, Eric D Morrell, Mark M Wurfel
COVID-19 is both a viral illness and a disease of immunopathology. Proximal events within the innate immune system drive the balance between deleterious inflammation and viral clearance. We hypothesize that a divergence between the generation of excessive inflammation through over activation of the TLR associated myeloid differentiation primary response (MyD88) pathway relative to the TIR-domain-containing
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Enteral nutrition ameliorates the symptoms of Crohn's disease in mice via activating special pro-resolving mediators through innate lymphoid cells Innate Immun. (IF 3.2) Pub Date : 2021-11-18 Di Zhao, Bo Yang, Chen Ye, Shaoyi Zhang, Xiaoqiong Lv, Qiyi Chen
Crohn's disease activates the inflammatory reactions to induce intestinal disorders. Enteral nutrition (EN) could exert general immunomodulatory effects. Cecal ligation and perforation (CLP) surgery was utilized to establish Crohn's disease mice models. Survival analysis, hematoxylin-eosin staining, flow cytometry, ELISA, Western blot and liquid chromatography-tandem MS were applied. Baicalein was
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Circulating innate lymphoid cell subtypes and altered cytokine profiles following an atherogenic high-fat diet Innate Immun. (IF 3.2) Pub Date : 2021-11-17 Vuyolwethu Mxinwa, Phiwayinkosi V. Dludla, Tawanda M. Nyambuya, Bongani B. Nkambule
Impaired Glc tolerance and hyperinsulinemia are a hallmark of type 2 diabetes (T2D) and are associated with an altered innate and adaptive immune response. In this study, we used a high-fat diet (HFD)-induced model of pre-diabetes to explore the pathological implications of altered innate lymphoid cell (ILC) profiles in a state of impaired Glc tolerance. Sixteen male C57BL/6 mice were randomized to
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Neglected roles of IgG Fc-binding protein secreted from airway mucin-producing cells in protecting against SARS-CoV-2 infection Innate Immun. (IF 3.2) Pub Date : 2021-09-15 Kensuke Kobayashi, Mitsuhiro Tachibana, Yutaka Tsutsumi
Both innate immunity and acquired immunity are involved in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. The induction of Abs that neutralize the virus has been described, and certain Abs against endemic coronaviruses may cross-react with SARS-CoV-2. Detailed mechanisms to protect against the pandemic of SARS-CoV-2 remain unresolved. We previously reported that IgG Fc-binding
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The TNF-α (–238 G/A) polymorphism could protect against development of severe sepsis Innate Immun. (IF 3.2) Pub Date : 2021-09-02 A. Hugo Montes, Eulalia Valle-Garay, Guadalupe Martin, Julio Collazos, Victoria Alvarez, Alvaro Meana, Laura Pérez-Is, José A. Carton, Francisco Taboada, Víctor Asensi
Primary responses in sepsis-mediated inflammation are regulated by pro-inflammatory cytokines. Variations in the cytokine genes might modify their transcription or expression, plasma cytokines levels and response to sepsis. Activation protein-1 (AP-1) and NF-κB regulate cytokines gene expression in sepsis. A total of 90 severely septic and 91 non-infected patients were prospectively studied. IL-1α
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Wolf–Hirschhorn syndrome candidate 1 facilitates alveolar macrophage pyroptosis in sepsis-induced acute lung injury through NEK7-mediated NLRP3 inflammasome activation Innate Immun. (IF 3.2) Pub Date : 2021-08-25 Caixia Liu, Benlong Cai, Dan Li, Yuan Yao
Sepsis is a complex clinical syndrome with high incidence and mortality. Acute lung injury (ALI) is a common complication of sepsis. At present, there is no effective therapeutic strategy to treat ALI. The SET domain–containing histone methyltransferase Wolf–Hirschhorn syndrome candidate 1 (WHSC1) regulates cancer progression, while its role in sepsis-induced ALI remains unclear. Thus, this study aimed
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Effects of fatty acid nitroalkanes on signal transduction pathways and airway macrophage activation Innate Immun. (IF 3.2) Pub Date : 2021-08-10 Melissa L Wilkinson, Andrew J Gow
Fatty acid nitroalkenes are reversibly-reactive electrophiles that are endogenously detectable at nM concentrations and display anti-inflammatory, pro-survival actions. These actions are elicited through the alteration of signal transduction proteins via a Michael addition on nucleophilic cysteine thiols. Nitrated fatty acids (NO2-FAs), like 9- or 10-nitro-octadec-9-enolic acid, will act on signal
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Long non-coding RNA ZFAS1 alleviates sepsis-induced myocardial injury via target miR-34b-5p/SIRT1 Innate Immun. (IF 3.2) Pub Date : 2021-08-02 Dan-Dan Chen, Hong-Wu Wang, Xing-Jun Cai
Long non-coding RNA ZFAS1 is down-regulated in sepsis. However, whether ZFAS1 participates in sepsis-induced cardiomyopathy (SIC) remains largely unknown. LPS injection to rats was used to establish an in vivo sepsis model, while LPS stimulation with H9C2 cell was used to mimic an in vitro sepsis-induced myocardial injury model. Western blots and quantitative RT-PCR were performed to evaluate protein
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Does chronic dietary exposure to the mycotoxin deoxynivalenol affect the porcine hepatic transcriptome when an acute-phase response is initiated through first or second-pass LPS challenge of the liver? Innate Immun. (IF 3.2) Pub Date : 2021-07-31 Sven Dänicke, Ann-Katrin Heymann, Michael Oster, Klaus Wimmers, Tanja Tesch, Erik Bannert, Susanne Bühler, Susanne Kersten, Jana Frahm, Jeannette Kluess, Stefan Kahlert, Hermann-Josef Rothkötter, Fabian Billenkamp
The sensitivity of pigs to deoxynivalenol (DON) might be increased by systemic inflammation (SI), which also has consequences for hepatic integrity. Liver lesions and a dys-regulated gene network might hamper hepatic handling and elimination of DON whereby the way of initiation of hepatic inflammation might play an additional role. First and second-pass exposure of the liver with LPS for triggering
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Synergistic effect of genetic polymorphisms in TLR6 and TLR10 genes on the risk of pulmonary tuberculosis in a Moldavian population Innate Immun. (IF 3.2) Pub Date : 2021-07-18 Alexander Varzari, Igor V. Deyneko, Elena Tudor, Harald Grallert, Thomas Illig
Polymorphisms in genes that control immune function and regulation may influence susceptibility to pulmonary tuberculosis (TB). In this study, 14 polymorphisms in 12 key genes involved in the immune response (VDR, MR1, TLR1, TLR2, TLR10, SLC11A1, IL1B, IL10, IFNG, TNF, IRAK1, and FOXP3) were tested for their association with pulmonary TB in 271 patients with TB and 251 community-matched controls from
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IRAK-4 in macrophages contributes to inflammatory osteolysis of wear particles around loosened hip implants Innate Immun. (IF 3.2) Pub Date : 2021-06-18 Yang-chun Zhang, Jian-hong Xiao, Shao-jie Deng, Guo-liang Yi
TLRs recognizing PAMPS play a role in local immunity and participate in implant-associated loosening. TLR-mediated signaling is primarily regulated by IL-1 receptor associated kinase-M (IRAK-M) negatively and IRAK-4 positively. Our previous studies have proved that wear particles promote endotoxin tolerance in macrophages by inducing IRAK-M. However, whether IRAK-4 is involved in inflammatory osteolysis
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miR-150 and SRPK1 regulate AKT3 expression to participate in LPS-induced inflammatory response Innate Immun. (IF 3.2) Pub Date : 2021-06-06 Yanfen Yao, Hong Wang, Xueqin Xi, Wei Sun, Junke Ge, Pibao Li
miR-150 was found to target the 3′-untranslated regions of AKT3, and the AKT pathway was affected by SR protein kinase 1 (SRPK1). However, the expression and significance of miR-150, AKT3 and SRPK1 in acute lung injury (ALI) were not clear. Here, we found that the expression of miR-150 was significantly reduced, while the expression of AKT3 and SRPK1 were markedly increased in LPS-treated A549, THP-1
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Implication of Toll/IL-1 receptor domain containing adapters in Porphyromonas gingivalis-induced inflammation Innate Immun. (IF 3.2) Pub Date : 2021-05-21 Isaac M Bugueno, Nadia Benkirane-Jessel, Olivier Huck
Periodontitis is induced by periodontal dysbiosis characterized by the predominance of anaerobic species. TLRs constitute the classical pathway for cell activation by infection. Interestingly, the Toll/IL-1 receptor homology domain adapters initiate signaling events, leading to the activation of the expression of the genes involved in the host immune response. The aim of this study was to evaluate
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Protective effects of recombinant 53-kDa protein of Trichinella spiralis on acute lung injury in mice via alleviating lung pyroptosis by promoting M2 macrophage polarization Innate Immun. (IF 3.2) Pub Date : 2021-05-20 Ling-yu Wei, An-qi Jiang, Ren Jiang, Si-ying Duan, Xue Xu, Ze-da-zhong Su, Jia Xu
Trichinella spiralis represents an effective treatment for autoimmune and inflammatory diseases. The effects of recombinant T. spiralis (TS) 53-kDa protein (rTsP53) on acute lung injury (ALI) remain unclear. Here, mice were divided randomly into a control group, LPS group, and rTsP53 + LPS group. ALI was induced in BALB/c mice by LPS (10 mg/kg) injected via the tail vein. rTsP53 (200 µl; 0.4 μg/μl)
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Baicalin attenuates LPS-induced alveolar type II epithelial cell A549 injury by attenuation of the FSTL1 signaling pathway via increasing miR-200b-3p expression Innate Immun. (IF 3.2) Pub Date : 2021-05-18 Xin-ya Duan, Yang Sun, Zhu-feng Zhao, Yao-qing Shi, Xun-yan Ma, Li Tao, Ming-wei Liu
In China, baicalin is the main active component of Scutellaria baicalensis, which has been used in the treatment of inflammation-related diseases, such as inflammation-induced acute lung injury. However, its specific mechanism remains unclear. This study examined the protective effect of baicalin on LPS-induced inflammation injury of alveolar epithelial cell line A549 and explored its protective mechanism
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Anti-HMGB1 auto-Abs influence fatigue in patients with Crohn’s disease Innate Immun. (IF 3.2) Pub Date : 2021-05-03 Ingeborg Kvivik, Tore Grimstad, Grete Jonsson, Jan T. Kvaløy, Roald Omdal
Fatigue is common in all chronic inflammatory and autoimmune diseases. A conceptual model for understanding the biological basis of fatigue describes it as being a part of the sickness behaviour response generated by pro-inflammatory cytokines and other mediators. We hypothesised that the pro-inflammatory high mobility group box 1 (HMGB1) protein is a fatigue-inducing molecule and that auto-Abs against
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TLR4 and TLR9 polymorphism: Probable role in susceptibility among the population of Bihar for Indian visceral leishmaniasis Innate Immun. (IF 3.2) Pub Date : 2021-04-28 Abhishek Mandal, Manish Kumar, Ashish Kumar, Abhik Sen, Pradeep Das, Sushmita Das
Genetic variations in the host TLRs genes play an important role in susceptibility and/or resistance to visceral leishmaniasis by altering the host-pathogen interaction. In this study, we investigated the association between polymorphisms of TLR4 (Asp299Gly, Thr399Ile) and TLR-9 (T-1237C), with susceptibility to visceral leishmaniasis. A bi-directional PCR amplification of specific alleles technique
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Synthetic glycolipid-based TLR4 antagonists negatively regulate TRIF-dependent TLR4 signalling in human macrophages Innate Immun. (IF 3.2) Pub Date : 2021-04-16 Charys Palmer, Fabio A Facchini, Richard PO Jones, Frank Neumann, Francesco Peri, Grisha Pirianov
TLRs, including TLR4, play a crucial role in inflammatory-based diseases, and TLR4 has been identified as a therapeutic target for pharmacological intervention. In previous studies, we investigated the potential of FP7, a novel synthetic glycolipid active as a TLR4 antagonist, to inhibit haematopoietic and non-haematopoietic MyD88-dependent TLR4 pro-inflammatory signalling. The main aim of this study