当前期刊: bioRxiv - Immunology Go to current issue    加入关注   
显示样式:        排序: IF: - GO 导出
我的关注
我的收藏
您暂时未登录!
登录
  • Predicted Cellular Immunity Population Coverage Gaps for SARS-CoV-2 Subunit Vaccines and their Augmentation by Compact Joint Sets
    bioRxiv. Immunol. Pub Date : 2020-08-06
    Ge Liu; Brandon Carter; David K. Gifford

    Subunit vaccines induce immunity to a pathogen by presenting a component of the pathogen and thus inherently limit the representation of pathogen peptides for cellular immunity based memory. We find that SARS-CoV-2 subunit peptides may not be robustly displayed by the Major Histocompatibility Complex (MHC) molecules in certain individuals. We introduce an augmentation strategy for subunit vaccines

    更新日期:2020-08-06
  • Loss-of-function of Fbxo10, encoding a post-translational regulator of BCL2 in lymphomas, has no discernible effect on BCL2 or B lymphocyte accumulation in mice
    bioRxiv. Immunol. Pub Date : 2020-08-05
    Etienne Masle-Farquhar; Amanda Russell; Yangguang Li; Fen Zhu; Lixin Rui; Robert Brink; Christopher Goodnow

    Regulation of the anti-apoptotic BCL2 protein determines cell survival and is frequently abnormal in B cell lymphomas. An evolutionarily conserved post-translational mechanism for over-expression of BCL2 in human B cell lymphomas and the BCL2 paralogue CED-9 in Caenorhabditis elegans results from loss-of-function mutations in human FBXO10 and its C.elegans paralogue DRE-1, a BCL2/CED-9-binding subunit

    更新日期:2020-08-06
  • Antigen-specific induction of CD4+CD8αα+ intraepithelial T lymphocytes by Bacteroidetes species
    bioRxiv. Immunol. Pub Date : 2020-08-05
    Djenet Bousbaine; Preksha Bhagchandani; Mariya London; Mark Mimee; Scott Olesen; Mathilde Poyet; Ross W. Cheloha; John Sidney; Jingjing Ling; Aaron Gupta; Timothy K. Lu; Alessandro Sette; Eric J Alm; Daniel Mucida; Angelina M. Bilate; Hidde L. Ploegh

    The microbiome contributes to the development and maturation of the immune system. In response to commensal bacteria, CD4+ T cells can differentiate into distinct functional subtypes with regulatory or effector functions along the intestine. Peripherally-induced Foxp3+-regulatory T cells (pTregs) maintain immune homeostasis at the intestinal mucosa by regulating effector T cell responses against dietary

    更新日期:2020-08-06
  • Recapitulation of HIV-1 Env-Antibody Coevolution in Macaques Leading to Neutralization Breadth
    bioRxiv. Immunol. Pub Date : 2020-08-05
    Ryan S Roark; Hui Li; Wilton B Williams; Hema Chug; Rosemarie D Mason; Jason Gorman; Shuyi Wang; Fang-Hua Lee; Juliette Rando; Mattia Bonsignori; Kwan-Ki Hwang; Kevin O Saunders; Kevin Wiehe; M. Anthony Moody; Peter T Hraber; Kshitij Wagh; Elena E Giorgi; Ronnie M Russell; Frederic Bibollet-Ruche; Weimin Liu; Jesse Connell; Andrew G Smith; Julia DeVoto; Alexander I Murphy; Jessica Smith; Wenge Ding;

    Neutralizing antibodies elicited by HIV-1 coevolve with viral Envs in distinctive patterns, in some cases acquiring substantial breadth. Here we show that primary HIV-1 Envs, when expressed by simian-human immunodeficiency viruses in rhesus macaques, elicited patterns of Env-antibody coevolution strikingly similar to those in humans. This included conserved immunogenetic, structural and chemical solutions

    更新日期:2020-08-06
  • PD-1 blockade exacerbates Mycobacterium tuberculosis infection in rhesus macaques
    bioRxiv. Immunol. Pub Date : 2020-08-05
    Keith D Kauffman; Shunsuke Sakai; Nickiana E Lora; Sivaranjani Namasivayam; Paul J Baker; Olena Kamenyeva; Taylor W Foreman; Christine E Nelson; Deivide Oliveira-de-Souza; Caian L Vinhaes; Ziv Yaniv; Cecilia S Lindestam Arleham; Alessandro Sette; Gordon J Freeman; Rashida Moore; the NIAID/DIR Tuberculosis Imaging Program; Alan Sher; Katrin D Mayer-Barber; Bruno B Andrade; Juraj Kabat; Laura E Via;

    Boosting immune cell function by targeting the co-inhibitory receptor PD-1 may have applications in the treatment of chronic infections. Here we examine the role of PD-1 during Mycobacterium tuberculosis (Mtb) infection of rhesus macaques. Animals treated with αPD-1 mAb developed worse disease and higher granuloma bacterial loads compared to isotype control treated monkeys. PD-1 blockade increased

    更新日期:2020-08-06
  • Post-Transcriptional Regulation of Antiviral Gene Expression by N6-Methyladenosine
    bioRxiv. Immunol. Pub Date : 2020-08-05
    Michael J McFadden; Alexa B. R. McIntyre; Haralambos Mourelatos; Nathan S Abell; Nandan S Gokhale; Hélène Ipas; Blerta Xhemalçe; Christopher E Mason; Stacy M Horner

    Type I interferons (IFN) induce hundreds of IFN-stimulated genes (ISGs) in response to viral infection. These ISGs require strict regulation for an efficient and controlled antiviral response, but post-transcriptional controls of these genes have not been well defined. Here, we identify a new role for the RNA base modification N6-methyladenosine (m6A) in the regulation of ISGs. Using ribosome profiling

    更新日期:2020-08-06
  • Alveolitis in severe SARS-CoV-2 pneumonia is driven by self-sustaining circuits between infected alveolar macrophages and T cells
    bioRxiv. Immunol. Pub Date : 2020-08-05
    Rogan A Grant; Luisa Morales-Nebreda; Nikolay S Markov; Suchitra Swaminathan; Estefany R Guzman; Darryl A Abbott; Helen K Donnelly; Alvaro Donayre; Isaac A Goldberg; Zasu M Klug; Nicole Borkowski; Ziyan Lu; Hermon Kihshen; Yuliya Politanska; Lango Sichizya; Mengjia Kang; Ali Shilatifard; Chao Qi; A Christine Argento; Jacqueline M Kruser; Elizabeth S Malsin; Chiagozie O Pickens; Sean Smith; James M

    Some patients infected with Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) develop severe pneumonia and the acute respiratory distress syndrome (ARDS). Distinct clinical features in these patients have led to speculation that the immune response to virus in the SARS-CoV-2-infected alveolus differs from other types of pneumonia. We collected bronchoalveolar lavage fluid samples from 86

    更新日期:2020-08-06
  • The DHODH Inhibitor PTC299 Arrests SARS-CoV-2 Replication and Suppresses Induction of Inflammatory Cytokines
    bioRxiv. Immunol. Pub Date : 2020-08-05
    Jeremy Luban; Rachel Sattler; Elke Muhlberger; Jason D. Graci; Liangxian Cao; Marla Weetall; Christopher Trotta; Joseph M. Colacino; Sina Bavari; Caterina Strambio-De-Castillia; Ellen L. Suder; Yetao Wang; Veronica Soloveva; Katherine Cintron-Lue; Nikolai A. Naryshkin; Mark Pykett; Ellen M. Welch; Kylie O'Keefe; Ronald Kong; Elizabeth Goodwin; Allan Jacobson; Slobodan Paessler; Stuart Peltz

    The coronavirus disease 2019 (COVID-19) pandemic has created an urgent need for therapeutics that inhibit the SARS-CoV-2 virus and suppress the fulminant inflammation characteristic of advanced illness. Here, we describe the anti-COVID-19 potential of PTC299, an orally available compound that is a potent inhibitor of dihydroorotate dehydrogenase (DHODH), the rate-limiting enzyme of the de novo pyrimidine

    更新日期:2020-08-06
  • Rapid GMP-compliant expansion of SARS-CoV-2-specific T cells from convalescent donors for use as an allogeneic cell therapy for COVID-19
    bioRxiv. Immunol. Pub Date : 2020-08-05
    Rachel S Cooper; Alasdair R Fraser; Linda Smith; Paul Burgoyne; Stuart N Imlach; Lisa M Jarvis; Sharon Zahara; Marc L Turner; John DM Campbell

    COVID-19 disease caused by the SARS-CoV-2 virus is characterized by dysregulation of effector T cells and accumulation of exhausted T cells. T cell responses to viruses can be corrected by adoptive cellular therapy using donor-derived virus-specific T cells. Here we show that SARS-CoV-2-exposed blood donations contain CD4 and CD8 memory T cells specific for SARS-CoV-2 spike, nucleocapsid and membrane

    更新日期:2020-08-06
  • Immune gene expression covaries with gut microbiome composition in stickleback
    bioRxiv. Immunol. Pub Date : 2020-08-05
    Lauren E Fuess; Stijn Den Haan; Fei Ling; Jesse N Weber; Natalie C Steinel; Daniel I Bolnick

    Commensal microbial communities have immense effects on their vertebrate hosts, contributing to a number of physiological functions as well as host fitness. In particular, host immunity is strongly linked to microbiota composition through poorly understood bi-directional links. Gene expression may be a potential mediator of these links between microbial communities and host function. However few studies

    更新日期:2020-08-06
  • Immunosuppressive role of PGE2 during human tuberculosis
    bioRxiv. Immunol. Pub Date : 2020-08-05
    Joaquin Miguel Pellegrini; Nancy Liliana Tateosian; Maria Paula Morelli; Agustin Rolandelli; Nicolas Oscar Amiano; Domingo Juan Palmero; Alberto Levi; Lorena Ciallella; Maria Isabel Colombo; Veronica Edith Garcia

    Prostaglandin E2 (PGE2), an active lipid compound derived from arachidonic acid, regulates different stages of the immune response of the host during several pathologies such as chronic infections or cancer. Manipulation of PGE2 levels was proposed as an approach for countering the Type I IFN signature of tuberculosis (TB), but very limited information exists about this pathway in patients with active

    更新日期:2020-08-06
  • Proteogenomic analysis unveils the HLA Class I presented immunopeptidome in melanoma and EGFR mutant lung adenocarcinoma
    bioRxiv. Immunol. Pub Date : 2020-08-05
    Yue A. Qi; Tapan K. Maity; Constance M. Cultraro; Vikram Misra; Xu Zhang; Catherine Ade; Shaojian Gao; David Milewski; Khoa D. Nguyen; Mohammad H. Ebrahimabadi; Ken-ichi Hanada; Javed Khan; Cenk Sahinalp; James C. Yang; Udayan Guha

    Immune checkpoint inhibitor and adoptive lymphocyte transfer-based therapies have shown great therapeutic potential for cancers with high tumor mutation burden (TMB). Here, we employed mass spectrometry (MS)-based proteogenomic large-scale profiling to identify potential immunogenic human leukocyte antigen (HLA) Class I-presented peptides in both melanoma, a hot tumor with high TMB, and EGFR mutant

    更新日期:2020-08-06
  • Anti-IL-6 versus Anti-IL-6R Blocking Antibodies to Treat Acute Ebola Infection in BALB/c Mice: Potential Implications for Treating Cytokine Release Syndrome
    bioRxiv. Immunol. Pub Date : 2020-08-04
    Reid Martin Rubsamen; Scott Burkholz; Shane Massey; Trevor Brasel; Tom Hodge; Lu Wang; Charles Herst; Richard Thomas Carback; Paul Harris

    Cytokine release syndrome (CRS) is known to be a factor in morbidity and mortality associated with acute viral infections including those caused by filoviruses and coronaviruses. IL-6 has been implicated as a cytokine negatively associated with survival after filovirus and coronavirus infection. However, IL-6 has also been shown to be an important mediator of innate immunity and important for the host

    更新日期:2020-08-05
  • Inflammasome- and gasdermin D-independent IL-1β production mobilizes neutrophils to inhibit antitumor immunity
    bioRxiv. Immunol. Pub Date : 2020-08-04
    Máté Kiss; Lieselotte Vande Walle; Els Lebegge; Helena Van Damme; Aleksandar Murgaski; Junbin Qian; Manuel Ehling; Samantha Pretto; Evangelia Bolli; Jiri Keirsse; Yvon Elkrim; Maria Solange Martins; Amelie Fossoul; Diether Lambrechts; Massimiliano Mazzone; Andy Wullaert; Mohamed Lamkanfi; Jo A. Van Ginderachter; Damya Laoui

    Interleukin-1β (IL-1β) is a central mediator of inflammation whose secretion typically requires proteolytic maturation by the inflammasome and formation of membrane pores by gasdermin D (GSDMD). Emerging evidence suggests an important role for IL-1β in promoting cancer progression in patients, but the underlying mechanisms are little understood. Here, we show a key role for IL-1β in driving tumor progression

    更新日期:2020-08-05
  • Interaction of clinical-stage antibodies with heme predicts physiochemical and functional liabilities relevant for developability
    bioRxiv. Immunol. Pub Date : 2020-08-04
    Maxime Lecerf; Alexia Kanyavuz; Sofia Rossini; Jordan D Dimitrov

    Development of monoclonal antibody therapeutics is a sophisticated process. Early stage detection of negative traits of antibodies, which can impact the production, storage stability or therapeutic efficacy is of critical importance for saving time and resources in drug development programs. Since the developability issues of antibodies are diverse, including expression yield, chemical and physical

    更新日期:2020-08-05
  • Lymphopenia-induced T cell proliferation is a hallmark of severe COVID-19
    bioRxiv. Immunol. Pub Date : 2020-08-04
    Sarah Adamo; Stéphane Chevrier; Carlo Cervia; Yves Zurbuchen; Miro E. Räber; Liliane Yang; Sujana Sivapatham; Andrea Jacobs; Esther Bächli; Alain Rudiger; Melina Stüssi-Helbling; Lars C. Huber; Dominik Schaer; Bernd Bodenmiller; Onur Boyman; Jakob Nilsson

    Coronavirus disease 2019 (COVID-19), caused by infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has a broad clinical presentation ranging from asymptomatic infection to fatal disease. Different features associated with the immune response to SARS-CoV-2, such as hyperinflammation and reduction of peripheral CD8+ T cell counts are strongly associated with severe disease. Here

    更新日期:2020-08-05
  • A distinct innate immune signature marks progression from mild to severe COVID-19
    bioRxiv. Immunol. Pub Date : 2020-08-04
    Stéphane Chevrier; Yves Zurbuchen; Carlo Cervia; Sarah Adamo; Miro E Raeber; Natalie de Souza; Sujana Sivapatham; Andrea Jacobs; Esther Bächli; Alain Rudiger; Melina Stüssi-Helbling; Lars C Huber; Dominik J Schaer; Jakob Nilsson; Onur Boyman; Bernd Bodenmiller

    Coronavirus disease 2019 (COVID-19) manifests with a range of severities, but immune signatures of mild and severe disease are still not fully understood. Excessive inflammation has been postulated to be a major factor in the pathogenesis of severe COVID-19 and innate immune mechanisms are likely to be central in the inflammatory response. We used 40-plex mass cytometry and targeted serum proteomics

    更新日期:2020-08-05
  • A valid protective immune response elicited in rhesus macaques by an inactivated vaccine is capable of defending against SARS-CoV-2 infection
    bioRxiv. Immunol. Pub Date : 2020-08-04
    Hongbo Chen; Zhongping Xie; Runxiang Long; Shengtao Fan; Heng Li; Zhanlong He; Kanwei Xu; Yun Liao; Lichun Wang; Ying Zhang; Xueqi Li; Xingq Dong; Tangwei Mou; Xiaofang Zhou; Yaoyun Yang; Lei Guo; Jianbo Yang; Huiwen Zheng; Xingli Xu; Jing Li; Yan Liang; Dandan Li; Zhimei Zhao; Chao Hong; Heng Zhao; Guorun Jiang; Yanchun Che; Fengmei Yang; Yunguang Hu; Xi Wang; Jing Pu; Kaili Ma; Lin Wang; Chen Chen;

    With the relatively serious global epidemic outbreak of SARS-CoV-2 infection, public concerns focus on not only clinical therapeutic measures and public quarantine for this disease but also the development of vaccines. The technical design of our SARS-CoV-2 inactivated vaccine provides a viral antigen that enables the exposure of more than one structural protein based upon the antibody composition

    更新日期:2020-08-05
  • Nasal commensal, Staphylococcus epidermidis shapes the mucosal environment to prevent influenza virus invasion through Serpine1 induction
    bioRxiv. Immunol. Pub Date : 2020-08-04
    Ara Jo; Jina Won; Chan Hee Gil; Jae Young Choi; Kang-Mu Lee; Sang Sun Yoon; Hyun Jik Kim

    Our recent study presented evidence that Staphylococcus epidermidis (S. epidermidis) was the most frequently encountered microbiome component in healthy human nasal mucus and that S. epidermidis could induce interferon (IFN)-dependent innate immunity to control acute viral lung infection. The serine protease inhibitor Serpine1 was identified to inhibit influenza A virus (IAV) spread by inhibiting glycoprotein

    更新日期:2020-08-05
  • Identification of a prostaglandin E2 receptor that regulates mosquito oenocytoid immune cell function in limiting bacteria and parasite infection
    bioRxiv. Immunol. Pub Date : 2020-08-04
    Hyeogsun Kwon; David R. Hall; Ryan C Smith

    Lipid-derived signaling molecules known as eicosanoids have integral roles in mediating immune and inflammatory processes across metazoans. This includes the function of prostaglandins and their cognate G protein-coupled receptors (GPCRs) to employ their immunological actions. In insects, prostaglandins have been implicated in the regulation of both cellular and humoral immune responses, yet studies

    更新日期:2020-08-05
  • Intestinal infection results in impaired lung innate immunity to secondary respiratory infection.
    bioRxiv. Immunol. Pub Date : 2020-08-04
    Shubhanshi Trivedi; Allie H Grossmann; Owen Jensen; Mark J Cody; Kristi J Warren; Christian C Yost; Daniel T Leung

    Pneumonia and diarrhea are among the leading causes of death worldwide, and epidemiological studies have demonstrated that diarrhea is associated with an increased risk of subsequent pneumonia. Our aim was to determine the impact of intestinal infection on innate immune responses in the lung. Using a mouse model of intestinal infection by Salmonella enterica serovar Typhimurium (S. Typhimurium), we

    更新日期:2020-08-05
  • The human liver microenvironment shapes the homing and function of CD4+ T-cell populations
    bioRxiv. Immunol. Pub Date : 2020-08-04
    Benjamin G Wiggins; Laura J Pallett; Xiaoyan Li; Scott P Davies; Oliver E Amin; Upkar S Gill; Arzoo M Patel; Konstantions Aliazis; Yuxin S Liu; Gary M Reynolds; Gideon Hirschfield; Patrick F Kennedy; Yuehua Huang; Mala M Maini; Zania Stamataki

    Background & Aims: Tissue-resident memory T cells (TRM) are important immune sentinels that provide efficient in situ immunity. Liver-resident CD8+ TRM have been previously described, and contribute to viral control in persistent hepatotropic infections. However, little is known regarding liver CD4+ TRM cells. Here we profiled resident and non-resident intrahepatic CD4+ T cell subsets, assessing their

    更新日期:2020-08-05
  • SARS-CoV-2-specific T cells exhibit unique features characterized by robust helper function, lack of terminal differentiation, and high proliferative potential.
    bioRxiv. Immunol. Pub Date : 2020-08-03
    Jason Neidleman,Xiaoyu Luo,Julie Frouard,Guorui Xie,Gill Gurjot,Ellen S Stein,Matthew McGregor,Tongcui Ma,Ashley F George,Astrid Kosters,Warner C Greene,Joshua Vasquez,Eliver Ghosn,Sulggi Lee,Nadia R Roan

    Convalescing COVID-19 patients mount robust T cell responses against SARS-CoV-2, suggesting an important role for T cells in viral clearance. To date, the phenotypes of SARS-CoV-2-specific T cells remain poorly defined. Using 38-parameter CyTOF, we phenotyped longitudinal specimens of SARS-CoV-2-specific CD4+ and CD8+ T cells from nine individuals who recovered from mild COVID-19. SARS-CoV-2-specific

    更新日期:2020-08-05
  • Treatment with commonly used antiretroviral drugs induces a type I/III interferon signature in the gut in the absence of HIV infection
    bioRxiv. Immunol. Pub Date : 2020-08-03
    Sean M. Hughes; Claire N. Levy; Fernanda Calienes; Joanne D. Stekler; Urvashi Pandey; Lucia Vojtech; Alicia R. Berard; Kenzie Birse; Laura Noel-Romas; Brian Richardson; Jackelyn B. Golden; Michael Cartwright; Ann C. Collier; Claire E. Stevens; Marcel E. Curlin; Timothy H. Holtz; Nelly Mugo; Elizabeth Irungu; Elly Katabira; Timothy Muwonge; Javier R Lama; Jared M. Baeten; Adam Burgener; Jairam R. Lingappa;

    Tenofovir disoproxil fumarate (TDF) and emtricitabine (FTC) are used for HIV treatment and prevention. Previously, we found that topical rectal tenofovir gel caused immunological changes in the mucosa. Here we assessed the effect of oral TDF/FTC in three HIV pre-exposure prophylaxis trials, two with gastrointestinal and one with cervicovaginal biopsies. TDF/FTC induced type I/III interferon-related

    更新日期:2020-08-04
  • Functional immune mapping with deep-learning enabled phenomics applied to immunomodulatory and COVID-19 drug discovery
    bioRxiv. Immunol. Pub Date : 2020-08-03
    Michael F Cuccarese; Berton A Earnshaw; Katie Heiser; Ben Fogelson; Chadwick T Davis; Peter F McLean; Hannah B Gordon; Kathleen-Rose Skelly; Fiona L Weathersby; Vlad Rodic; Ian K Quigley; Elissa D Pastuzyn; Brandon M Mendivil; Nathan H Lazar; Carl A Brooks; Joseph Carpenter; Pamela Jacobson; Seth W Glazier; Jes Ford; James D Jensen; Nicholas D Campbell; Michael A Statnick; Adeline S Low; Kirk R Thomas;

    Development of accurate disease models and discovery of immune-modulating drugs is challenged by the immune system's highly interconnected and context-dependent nature. Here we apply deep-learning-driven analysis of cellular morphology to develop a scalable 'phenomics' platform and demonstrate its ability to identify dose-dependent, high-dimensional relationships among and between immunomodulators

    更新日期:2020-08-04
  • Peritoneal macrophage phenotype correlates with pain scores in women with suspected endometriosis
    bioRxiv. Immunol. Pub Date : 2020-08-03
    Douglas A Gibson; Frances Collins; Bianca De Leo; Andrew W Hotne; Philippa Saunders

    Objective: To characterise peritoneal macrophage populations in women with suspected endometriosis and assess if they are correlated with severity of pelvic pain symptoms. Design: Flow cytometry analysis of peritoneal fluid samples and clinical data. Setting: University Research Institute. Patients: Clinical questionnaires, surgical data and peritoneal fluid were collected with informed consent from

    更新日期:2020-08-04
  • Antibody:CD47 ratio regulates macrophage phagocytosis through competitive receptor phosphorylation
    bioRxiv. Immunol. Pub Date : 2020-08-02
    Emily C Suter; Eva M Schmid; Erik Voets; Brian Francica; Daniel A Fletcher

    Cancer immunotherapies often modulate macrophage effector function by introducing either targeting antibodies that activate Fc gamma receptors or blocking antibodies that disrupt inhibitory SIRPα-CD47 engagement. Yet how these competing signals are integrated is poorly understood mechanistically, raising questions about how to effectively titrate immune responses. Here we find that macrophage phagocytic

    更新日期:2020-08-02
  • Multiomic Immunophenotyping of COVID-19 Patients Reveals Early Infection Trajectories
    bioRxiv. Immunol. Pub Date : 2020-07-31
    Yapeng Su; Daniel Chen; Christopher Lausted; Dan Yuan; Jongchan Choi; Cheng Dai; Valentin Voillet; Kelsey Scherler; Pamela Troisch; Venkata R Duvvuri; Priyanka Baloni; Guangrong Qin; Brett Smith; Sergey Kornilov; Clifford Rostomily; Alex Xu; Jing Li; Shen Dong; Alissa Rothchild; Jing Zhou; Kim Murray; Rick Edmark; Sunga Hong; Lesley Jones; Yong Zhou; Ryan Roper; Sean Mackay; D. Shane O'Mahony; Christopher

    Host immune responses play central roles in controlling SARS-CoV2 infection, yet remain incompletely characterized and understood. Here, we present a comprehensive immune response map spanning 454 proteins and 847 metabolites in plasma integrated with single-cell multi-omic assays of PBMCs in which whole transcriptome, 192 surface proteins, and T and B cell receptor sequence were co-analyzed within

    更新日期:2020-08-01
  • Baseline pulmonary levels of CD8+ T cells and NK cells inversely correlate with expression of the SARS-CoV-2 entry receptor ACE2.
    bioRxiv. Immunol. Pub Date : 2020-07-31
    Pascal H G Duijf

    COVID-19 is caused by the coronavirus SARS-CoV-2 and currently has detrimental human health, community and economic impacts around the world. It is unclear why some SARS-CoV-2-positive individuals remain asymptomatic, while others develop severe symptoms. Baseline pulmonary levels of anti-viral leukocytes, already residing in the lung prior to infection, may orchestrate an effective early immune response

    更新日期:2020-08-01
  • Tolerogenic nanoparticles targeting B and T lymphocytes delay autoimmune arthritis in K/BxN mice
    bioRxiv. Immunol. Pub Date : 2020-07-31
    Amrita Srivastava; Britni M Arlian; Takashi K Kishimoto; James C. Paulson

    Current treatments for unwanted antibody responses largely rely on immunosuppressive drugs compromising overall immunity. New approaches to achieve antigen-specific tolerance are desirable to avoid unwanted side effects. Several nanoparticle-based approaches designed to tolerize the B or T cell arms of the humoral immune response have shown promise for induction of antigen-specific tolerance, raising

    更新日期:2020-08-01
  • Expression of Tim-3 drives naive Treg to an effector-like state with enhanced suppressive activity
    bioRxiv. Immunol. Pub Date : 2020-07-31
    Hridesh Banerjee; Hector Nieves-Rosado; Aditi Kulkarni; Benjamin Murter; Uma R Chandran; Alexander Chang; Andrea L Szymczak-Workman; Lazar Vujanovic; Robert L Ferris; Lawrence P. Kane

    Regulatory T cells (Treg) are critical mediators of self-tolerance but can also limit effective anti-tumor immunity. We and others previously reported that 40-60% percent of Treg-infiltrating head and neck cancer (HNC) and other tumors highly express Tim-3, compared with about 5% in lymphoid organs. Tumor-infiltrating Tim-3+ Treg also have enhanced suppressive function and display a more effector-like

    更新日期:2020-08-01
  • Exaggerated in vivo IL-17 responses discriminate recall responses in active TB
    bioRxiv. Immunol. Pub Date : 2020-07-31
    Gabriele Pollara; Carolin T Turner; Gillian S Tomlinson; Lucy CK Bell; Ayesha Khan; Luis Felipe Peralta; Anna Folino; Ayse Akarca; Cristina Venturini; Tina Baker; Fabio LM Ricciardolo; Teresa Marafioti; Cesar Ugarte-Gil; David AJ Moore; Benjamin M Chain; Mahdad Noursadeghi

    Background: Host immune responses at the site of Mycobacterium tuberculosis (Mtb) infection serve to contain the pathogen, but also mediate the pathogenesis of tuberculosis (TB) and onward transmission of infection. Based on the premise that active TB disease is predominantly a manifestation of immunopathology, we tested the hypothesis that immune responses at the site of host-pathogen interactions

    更新日期:2020-08-01
  • Disordered peptides impair neutrophil bacterial clearance and enhance tissue damage in septic patients
    bioRxiv. Immunol. Pub Date : 2020-07-31
    Csaba I Timar; Ferenc Kolonics; Viktor Berzsenyi; Eszter Tamaska; Anna Parkanyi; Michael L Merchant; Daniel W Wilkey; Zsolt Ivanyi; Kenneth R McLeish; Erzsebet Ligeti

    Neutrophilic granulocytes are required for antimicrobial defense, but they can also be harmful to the host organism. The current study demonstrates that disordered peptides in the 3-12 kDa size range in the plasma of septic patients alter effector functions of neutrophils from healthy donors. Those peptides stimulated exocytosis, increased extracellular release of reactive oxygen species (ROS), decreased

    更新日期:2020-08-01
  • Transcriptional Atlas of Ileal-Anal Pouch Immune Cells from Ulcerative Colitis Patients
    bioRxiv. Immunol. Pub Date : 2020-07-31
    Joseph Cooper Devlin; Jordan Axelrad; Ashley Hine; Shannon Chang; Suparna Sarkar; Jian-Da Lin; Kelly Valentine Ruggles; David Hudesman; Ken Cadwell; P'ng Loke

    How the human intestinal immune system is distinctly organized to respond to inflammation is still poorly understood. Here, we used single-cell RNA-sequencing to examine lamina propria CD45+ hematopoietic cells from patients with inflammatory bowel disease that have undergone ileal pouch-anal anastomosis, or the colon mucosa of ulcerative colitis patients. We identified a population of IL1B+ antimicrobial

    更新日期:2020-08-01
  • Validation and Application of a Bench Top Cell Sorter in a BSL-3 Containment Setting
    bioRxiv. Immunol. Pub Date : 2020-07-31
    Lydia M Roberts; Rebecca Anderson; Aaron Carmody; Catharine M Bosio

    Rigorous assessment of the cellular and molecular changes during infection typically requires isolation of specific immune cell subsets for downstream application. While there are numerous options for enrichment/isolation of cells from tissues, fluorescent activated cell sorting (FACS) is accepted as a method that results in superior purification of a wide variety of cell types. Flow cytometry requires

    更新日期:2020-08-01
  • Cryo-EM Structures Delineate a pH-Dependent Switch that Mediates Endosomal Positioning of SARS-CoV-2 Spike Receptor-Binding Domains
    bioRxiv. Immunol. Pub Date : 2020-07-31
    Tongqing Zhou; Yaroslav Tsybovsky; Adam S. Olia; Jason Gorman; Micah A. Rapp; Gabriele Cerutti; Gwo-Yu Chuang; Phinikoula S. Katsamba; Alexandra Nazzari; Jared M. Sampson; Arne Schon; Pengfei D. Wang; Jude Bimela; Wei Shi; I-Ting Teng; Baoshan Zhang; Jeffrey C. Boyington; Mallika Sastry; Tyler Stephens; Jonathan Stuckey; Shuishu Wang; Richard A. Friesner; David D. Ho; John R. Mascola; Lawrence Shapiro;

    The SARS-CoV-2 spike employs mobile receptor-binding domains (RBDs) to engage the ACE2 receptor and to facilitate virus entry. Antibodies can engage RBD but some, such as CR3022, fail to inhibit entry despite nanomolar spike affinity. Here we show the SARS-CoV-2 spike to have low unfolding enthalpy at serological pH and up to 10-times more unfolding enthalpy at endosomal pH, where we observe significantly

    更新日期:2020-08-01
  • Rbx1 Cullin-Ring ligase E3 and Ube2m neddylation E2 differentially governs the fitness of Treg cells
    bioRxiv. Immunol. Pub Date : 2020-07-31
    Di Wu; Haomin Li; Mingwei Liu; Jun Qin; Yi Sun

    Cullin-RING Ligases (CRLs) are a family of multi-unit E3 ubiquitin ligases with two members of RING family proteins, acting as the catalytic subunit: RING-box 1 (Rbx1) couples with CRLs1-4, whereas RING-box 2 (Rbx2/Sag) couples mainly with CRL5. Activity of CRLs requires neddylation on their Cullin subunit, catalyzed by neddylation enzyme cascades E1, E2 and E3. Ube2m and Ube2f are two neddylation

    更新日期:2020-08-01
  • SARS-CoV-2 protein subunit vaccination elicits potent neutralizing antibody responses
    bioRxiv. Immunol. Pub Date : 2020-07-31
    Marco Mandolesi; Daniel J Sheward; Leo Hanke; Junjie Ma; Pradeepa Pushparaj; Laura Perez Vidakovics; Changil Kim; Karin Loré; Xaquin Castro Dopico; Jonathan M Coquet; Gerald McInerney; Gunilla B Karlsson Hedestam; Ben Murrell

    The outbreak and spread of SARS-CoV-2 (Severe Acute Respiratory Syndrome coronavirus 2), the cause of coronavirus disease 2019 (COVID-19), is a current global health emergency and a prophylactic vaccine is needed urgently. The spike glycoprotein of SARS-CoV-2 mediates entry into host cells, and thus is a target for neutralizing antibodies and vaccine design. Here we show that adjuvanted protein immunization

    更新日期:2020-08-01
  • CD20 as a gatekeeper of the resting stage of human B cells
    bioRxiv. Immunol. Pub Date : 2020-07-31
    Kathrin Klasener; Julia Jellusova; Geoffroy Andrieux; Ulrich Salzer; Chiara Boehler; Sebastian N Steiner; Jonas B Albinus; Marco F Cavallari; Beatrix Suess; Melanie Boerries; Reinhard E Voll; Bernd Wollscheid; Michael Reth

    CD20 is a B cell specific membrane protein and a target of therapeutic antibodies such as rituximab (RTX). In spite of the prominent usage of anti-CD20 antibodies in the clinic little is known about the biological function of CD202. Here we show that CD20 controls the nanoscale organization of receptors on the surface of resting B lymphocytes. A CRISPR/Cas-based ablation of CD20 in Ramos B cells results

    更新日期:2020-08-01
  • Constitutive androstane receptor directs T cell adaptation to bile acids in the small intestine
    bioRxiv. Immunol. Pub Date : 2020-07-30
    Mei Lan Chen; Xiangsheng Huang; Hongtao Wang; Yujin Liu; Jinsai Shang; Amber Eliason; Courtney Hegner; HaJeung Park; Blake Frey; Guohui Wang; Douglas J. Kojetin; Casey T. Weaver; Matthew E. Pipkin; David D. Moore; Mark S. Sundrud

    Bile acids (BAs) are fundamental lipid emulsifying metabolites synthesized in hepatocytes and maintained via enterohepatic circulation between the liver and ileum. Because they are lipophilic, BAs can be cytotoxic in enterohepatic tissues and several nuclear receptors are now recognized for suppressing BA toxicity in hepatocytes and enterocytes. By contrast, it remains unclear how mucosal immune cells

    更新日期:2020-07-31
  • Postnatal expansion, maturation and functionality of MR1 T cells in humans
    bioRxiv. Immunol. Pub Date : 2020-07-30
    Gwendolyn M. Swarbrick; Anele Gela; Meghan E. Cansler; Megan D. Null; Rowan B. Duncan; Elisa Nemes; Muki Shey; Mary Nsereko; Harriet Mayanja-Kizza; Sarah Kiguli; Jeffrey Koh; Willem Hanekom; Mark Hatherill; Christina Lancioni; David M. Lewinsohn; Thomas Scriba; Deborah A. Lewinsohn

    MR1-restricted T (MR1T) cells are defined by their recognition of metabolite antigens presented by the monomorphic MHC class 1-related molecule, MR1, the most highly conserved MHC class I related molecule in mammalian species. Mucosal-associated invariant T (MAIT) cells are the predominant subset of MR1T cells expressing an invariant TCR alpha-chain, TRAV1-2. These cells comprise a T cell subset that

    更新日期:2020-07-31
  • A Multi-Phenotype System to Discover Therapies for Age-Related Dysregulation of the Immune Response to Viral Infections
    bioRxiv. Immunol. Pub Date : 2020-07-30
    Brandon W White; Ben Komalo; Lauren Nicolaisen; Matt Donne; Charlie Marsh; Rachel M DeVay; An M Nguyen; Wendy Cousin; Jarred Heinrich; William J Van Trump; Tempest Plott; Colin J Fuller; Dat Nguyen; Daneil Chen; Delia Bucher; Sabine Tyrra; Laura Haynes; George Kuchel; Jorge Goronzy; Anis Larbi; Tamas Fulop; Diane Heiser; Ralf Schwandner; Christian Elabd; Ben Kamens

    Age-related immune dysregulation contributes to increased susceptibility to infection and disease in older adults. We combined high-throughput laboratory automation with machine learning to build a multi-phenotype aging profile that models the dysfunctional immune response to viral infection in older adults. From a single well, our multi-phenotype aging profile can capture changes in cell composition

    更新日期:2020-07-31
  • Ad26-vector based COVID-19 vaccine encoding a prefusion stabilized SARS-CoV-2 Spike immunogen induces potent humoral and cellular immune responses
    bioRxiv. Immunol. Pub Date : 2020-07-30
    Rinke Bos; Lucy Rutten; Joan E.M. van der Lubbe; Mark J.G. Bakkers; Gijs Hardenberg; Frank Wegmann; David Zuijdgeest; Adriaan H. de Wilde; Annemart Koornneef; Annemiek Verwilligen; Danielle van Manen; Ted Kwaks; Ronald Vogels; Tim J. Dalebout; Sebenzile K Myeni; Marjolein Kikkert; Eric J Snijder; Zhenfeng Li; Dan H. Barouch; Vellinga Jort; Johannes PM Langedijk; Roland C Zahn; Jerome Custers; Hanneke

    Development of effective preventative interventions against SARS-CoV-2, the etiologic agent of COVID-19 is urgently needed. The viral surface spike (S) protein of SARS-CoV-2 is a key target for prophylactic measures as it is critical for the viral replication cycle and the primary target of neutralizing antibodies. We evaluated design elements previously shown for other coronavirus S protein-based

    更新日期:2020-07-31
  • Circulating Mitochondrial DNA is an Early Indicator of Severe Illness and Mortality from COVID-19
    bioRxiv. Immunol. Pub Date : 2020-07-30
    Davide Scozzi; Marlene Cano; Lina MA; Dequan Zhou; Ji Hong Zhu; Jane A O'Halloran; Charles Goss; Adriana Rauseo; Zhiyi Liu; Valentina Peritore; Monica Rocco; Alberto Ricci; Rachele Amodeo; Laura Aimati; Mohsen Ibrahim; Ramsey Hachem; Daniel Kreisel; Philip A Mudd; Hrishikesh S Kulkarni; Andrew E Gelman

    Mitochondrial DNA (MT-DNA) are intrinsically inflammatory nucleic acids released by damaged solid organs. Whether circulating MT-DNA levels could be used to predict the development of poor COVID-19 outcomes remains undetermined. Here, we measured circulating MT-DNA levels in prospectively collected, cell-free plasma samples from 97 subjects with COVID-19 at the time of hospital presentation. Circulating

    更新日期:2020-07-31
  • Cross-reactivity of SARS-CoV structural protein antibodies against SARS-CoV-2
    bioRxiv. Immunol. Pub Date : 2020-07-30
    Timothy A Bates; Jules B Weinstein; Scotland E Farley; Hans C Leier; William B Messer; Fikadu G Tafesse

    There is currently a lack of biological tools to study the replication cycle and pathogenesis of SARS-CoV-2, the etiological agent of COVID-19. Repurposing the existing tools, including antibodies of SARS-CoV, is an effective way to accelerate the development of therapeutics for COVID-19. Here, we extensively characterized antibodies of the SARS-CoV structural proteins for their cross-reactivity, experimental

    更新日期:2020-07-31
  • Systemic sterile induced-co-expression of IL-12 and IL-18 drives IFN-γ-dependent activation of microglia and recruitment of MHC-II-expressing inflammatory monocytes into the brain.
    bioRxiv. Immunol. Pub Date : 2020-07-30
    Emilia A. Gaviglio; Javier M. Peralta Ramos; Daniela S. Arroyo; Claudio Bussi; Pablo Iribarren; Maria C. Rodriguez-Galan

    The development of neuroinflammation, as well as the progression of several neurodegenerative diseases, has been associated with the activation and mobilization of the peripheral immune system due to systemic inflammation. However, the mechanism by which this occurs remains unclear. Herein, we addressed the effect of systemic, endotoxin-free induced-co-expression of IL-12 and IL-18 in the establishment

    更新日期:2020-07-31
  • Immuno-informatics Design of a Multimeric Epitope Peptide Based Vaccine Targeting SARS-CoV-2 Spike Glycoprotein
    bioRxiv. Immunol. Pub Date : 2020-07-30
    Onyeka S. Chukwudozie; Clive M. Gray; Tawakalt A. Fagbayi; Rebecca C. Chukwuanukwu; Victor O. Oyebanji; Taiwo T. Bankole; Richard A Adewole; Daniel M Eze

    Developing an efficacious vaccine to SARS-CoV-2 infection is critical to stem COVID-19 fatalities and providing the global community with immune protection. We have used a bioinformatic approach to aid in the design of an epitope peptide-based vaccine against the spike protein of the virus. Five antigenic B cell epitopes with viable antigenicity and a total of 27 discontinuous B cell epitopes were

    更新日期:2020-07-31
  • A Next Generation Bivalent Human Ad5 COVID-19 Vaccine Delivering Both Spike and Nucleocapsid Antigens Elicits Th1 Dominant CD4+, CD8+ T-cell and Neutralizing Antibody Responses
    bioRxiv. Immunol. Pub Date : 2020-07-30
    Adrian Rice; Mohit Verma; Annie Shin; Lise Zakin; Peter Sieling; Shiho Tanaka; Helty Adisetiyo; Justin Taft; Roosheel Sandeep Patel; Sofija Buta; Marta Martin-Fernandez; Brett Morimoto; Elizabeth Gabitzsch; Jeffrey T Safrit; Joseph Balint; Kyle Dinkins; Patricia Spilman; Dusan Bogunovic; Shahrooz Rabizadeh; Kayvan Niazi; Patrick Soon-Shiong

    In response to the health crisis presented by the COVID-19 pandemic, rapid development of safe and effective vaccines that elicit durable immune responses is imperative. Recent reports have raised the concern that antibodies in COVID-19 convalescent patients may not be long lasting and thus even these individuals may require vaccination. Vaccine candidates currently in clinical testing have focused

    更新日期:2020-07-31
  • No SARS-CoV-2 cross-neutralization by intravenous immunoglobulins produced from plasma collected before the 2020 pandemic
    bioRxiv. Immunol. Pub Date : 2020-07-30
    Julia Schwaiger; Michael Karbiener; Claudia Aberham; Maria R. Farcet; Thomas R. Kreil

    The 2020 SARS-CoV-2 pandemic is caused by a zoonotic coronavirus transmitted to humans, similar to earlier events. Whether the other, seasonally circulating coronaviruses induce cross-reactive, potentially even cross-neutralizing antibodies to the new species in humans is unclear. The question is of particular relevance for people with immune deficiencies, as their health depends on treatment with

    更新日期:2020-07-31
  • Biochemical Patterns of Antibody Polyreactivity Revealed Through a Bioinformatics-Based Analysis of CDR Loops
    bioRxiv. Immunol. Pub Date : 2020-07-30
    Christopher T. Boughter; Marta T. Borowska; Jenna J. Guthmiller; Albert Bendelac; Patrick Wilson; Benoit Roux; Erin J. Adams

    Antibodies are critical components of adaptive immunity, binding with high affinity to pathogenic epitopes. Antibodies undergo rigorous selection to achieve this high affinity, yet some maintain an additional basal level of low affinity, broad reactivity to diverse epitopes, a phenomenon termed "polyreactivity". While polyreactivity has been observed in antibodies isolated from various immunological

    更新日期:2020-07-31
  • E-cigarettes compromise the gut barrier and trigger gut inflammation
    bioRxiv. Immunol. Pub Date : 2020-07-30
    Aditi Sharma; Jasper Lee; Ayden Fonseca; Alex Moshensky; Taha Kothari; Ibrahim Sayed Ibrahim; Stella-Rita Ibeawuchi; Rama Pranadinata; Jason Ear; Debashis Sahoo; Laura Crotty-Alexander; Pradipta Ghosh; Soumita Das

    E-cigarette and vaping device use continue to rise, particularly in adolescents and young adults, but the safety of inhaling the multitude of chemicals within e-cigarette aerosols has been questioned. While several studies have evaluated vaping effects on the lungs and heart; effects on the gastrointestinal tract remain unknown. Using established murine models of acute (1 week) and chronic (3 month)

    更新日期:2020-07-31
  • Mapping the landscape of chromatin dynamics during naïve CD4+ T-cell activation.
    bioRxiv. Immunol. Pub Date : 2020-07-30
    Muhammad Munir Iqbal; Michael Serralha; Parwinder Kaur; David Martino

    T-cell activation induces context-specific gene expression programs that promote energy generation and biosynthesis, progression through the cell cycle and ultimately cell differentiation. The aim of this study was to apply the omni ATAC-seq method to characterize the landscape of chromatin changes induced by T-cell activation in mature naïve CD4+ T-cells. Using a well-established ex vivo protocol

    更新日期:2020-07-31
  • Genetically Determined Strength of Natural Killer Cells is Enhanced by Adaptive HLA class I Admixture in East Asians
    bioRxiv. Immunol. Pub Date : 2020-07-30
    Zhihui Deng; Jianxin Zhen; Genelle Francis Harrison; Guobin Zhang; Rui Chen; Ge Sun; Qiong Yu; Neda Nemat-Gorgani; Lisbeth A Guethlein; Liumei He; Mingzhong Tang; Xiaojiang Gao; Siqi Cai; Jonathan A Shortt; Christopher R Gignoux; Mary Carrington; Hongyan Zou; Peter Parham; Wenxu Hong; Paul J Norman

    Human natural killer (NK) cells are essential for controlling infection, cancer and fetal development. NK cell functions are modulated by interactions between polymorphic inhibitory killer cell immunoglobulin-like receptors (KIR) and polymorphic HLA-A, -B and -C ligands expressed on tissue cells. All HLA-C alleles encode a KIR ligand and contribute to reproduction and immunity. In contrast, only some

    更新日期:2020-07-30
  • Regulatory T cell activation triggers specific changes in glycosylation associated with Siglec-1-dependent inflammatory responses
    bioRxiv. Immunol. Pub Date : 2020-07-30
    Gang Wu; Gavuthami Murugesan; Manjula Nagala; Alex McCraw; Stuart M Haslam; Anne Dell; Paul R Crocker

    Siglec-1 is a macrophage lectin-like receptor that mediates sialic acid-dependent cellular interactions. It was shown previously to promote inflammation in autoimmune disease through suppressing the expansion of regulatory T cells (Tregs). We have investigated the molecular basis for Siglec-1 binding to these cells using in vitro-induced Tregs. Siglec-1 binding was strongly upregulated on activated

    更新日期:2020-07-30
  • How do extracellular membrane-associated proteins stand erect and signal?
    bioRxiv. Immunol. Pub Date : 2020-07-30
    Swetha Lankipalli; Udupi A Ramagopal

    Even after decades of research, a comprehensive mechanism that elucidates the underpinnings of signalling through the cell membrane is still elusive. Here, we address a simple question -how do the ectodomain of a membrane-associated protein consisting of multiple domains and connected by flexible linkers stand upright on the membrane?. Our analysis based on large amount of available functional and

    更新日期:2020-07-30
  • NK cells integrate signals over large areas when building immune synapses but require local stimuli for degranulation
    bioRxiv. Immunol. Pub Date : 2020-07-30
    Quentin Verron; Elin Forslund; Ludwig Brandt; Mattias Leino; Thomas W Frisk; Per E Olofsson; Björn Önfelt

    Immune synapses are large-scale, transient molecular assemblies that serve as platforms for antigen presentation to B and T cells, and target recognition by cytotoxic T cells and natural killer (NK) cells. The formation of an immune synapse is a tightly regulated, stepwise process where the cytoskeleton, cell-surface receptors and signaling proteins rearrange into supramolecular activation clusters

    更新日期:2020-07-30
  • Identification and characterization of CD4+ T cell epitopes after Shingrix vaccination.
    bioRxiv. Immunol. Pub Date : 2020-07-29
    Hannah Voic; Rory Dylan de Vries; John Sidney; Paul Rubiro; Erin Moore; Elizabeth J Phillips; Simon Mallal; Brittaney Schwan; Daniela Weiskopf; Alba Grifoni; Alessandro Sette

    Infections with varicella zoster virus (VZV), a member of the Herpesviridae family, are associated with a range of clinical manifestations. Primary infection with VZV causes chicken pox, and due to the virus capacity to remain latent in neurons, it can reactivate later in life causing herpes zoster (HZ), also known as shingles. Two different licensed vaccines are available to prevent HZ, either based

    更新日期:2020-07-30
  • Enhanced BCR signalling inflicts early plasmablast and germinal centre B cell death
    bioRxiv. Immunol. Pub Date : 2020-07-29
    Juan Carlos Yam-Puc; Lingling Zhang; Raul Antonio Maqueda-Alfaro; Laura Garcia-Ibanez; Yang Zhang; Jessica Davies; Yotis A Senis; Mike Snaith; Kai-Michael Toellner

    It is still not clear how B-cell receptor (BCR) signalling intensity affects plasma cell and germinal centre (GC) B cell differentiation. We generated Cg1Cre/+Ptpn6fl/fl mice where SHP-1, a negative regulator of BCR signalling, is deleted rapidly after B cell activation. Although immunisation with T-dependent antigens increased BCR signalling, it led to plasma cells reduction and increased apoptosis

    更新日期:2020-07-30
  • A test of the Baldwin Effect: Differences in both constitutive expression and inducible responses to parasites underlie variation in host response to a parasite
    bioRxiv. Immunol. Pub Date : 2020-07-29
    Lauren E Fuess; Jesse N Weber; Stijn den Haan; Natalie C Steinel; Kum Chuan Shim; Daniel I Bolnick

    Despite the significant effect of host-parasite interactions on both ecological systems and organism health, there is still limited understanding of the mechanisms driving evolution of host resistance to parasites. One model of rapid evolution, the Baldwin Effect, describes the role of plasticity in adaptation to novel conditions, and subsequent canalization of associated traits. While mostly applied

    更新日期:2020-07-30
  • Intradermal-delivered DNA vaccine provides anamnestic protection in a rhesus macaque SARS-CoV-2 challenge model
    bioRxiv. Immunol. Pub Date : 2020-07-29
    Ami Patel; Jewell Walters; Emma L Reuschel; Katherine Schultheis; Elizabeth Parzych; Ebony N Gary; Igor Maricic; Mansi Purwar; Zeena Eblimit; Susanne N Walker; Diana Guimet; Pratik Bhojnagarwala; Arthur Doan; Ziyang Xu; Dustin Elwood; Sophia M Reeder; Laurent Pessaint; Kevin Y Kim; Anthony Cook; Neethu Chokkalingam; Brad Finneyfrock; Edgar Tello-Ruiz; Alan Dodson; Jihae Choi; Alison Generotti; John

    Coronavirus disease 2019 (COVID-19), caused by the SARS-CoV-2 virus, has had a dramatic global impact on public health, social, and economic infrastructures. Here, we assess immunogenicity and anamnestic protective efficacy in rhesus macaques of the intradermal (ID)-delivered SARS-CoV-2 spike DNA vaccine, INO-4800. INO-4800 is an ID-delivered DNA vaccine currently being evaluated in clinical trials

    更新日期:2020-07-30
Contents have been reproduced by permission of the publishers.
导出
全部期刊列表>>
欢迎访问IOP中国网站
自然职场线上招聘会
GIANT
产业、创新与基础设施
自然科研线上培训服务
材料学研究精选
胸腔和胸部成像专题
屿渡论文,编辑服务
何川
苏昭铭
陈刚
姜涛
李闯创
李刚
北大
隐藏1h前已浏览文章
课题组网站
新版X-MOL期刊搜索和高级搜索功能介绍
ACS材料视界
天合科研
x-mol收录
上海纽约大学
陈芬儿
厦门大学
何振宇
史大永
吉林大学
卓春祥
张昊
杨中悦
试剂库存
down
wechat
bug