当前期刊: bioRxiv - Immunology Go to current issue    加入关注   
显示样式:        排序: IF: - GO 导出
我的关注
我的收藏
您暂时未登录!
登录
  • Immune Response Drives Outcomes in Prostate Cancer: Implications for Immunotherapy
    bioRxiv. Immunol. Pub Date : 2020-05-29
    Jialin Meng; Yujie Zhou; Xiaofan Lu; Zichen Bian; Yiding Chen; Song Fan; Jun Zhou; Li Zhang; Zongyao Hao; Meng Zhang; Chaozhao Liang

    Background: The heterogeneity of the immune microenvironment leads to the different response results of immune checkpoint blockade therapy. We aimed to propose a robust molecular classification of prostate cancer microenvironment to identify ideal patients for delivering effective immunotherapy. Methods: A total of 1,557 prostate cancer patients were enrolled in the current study, including 69 real-world

    更新日期:2020-05-29
  • Plasmin cascade mediates thrombolytic events in SARS-CoV-2 infection via complement and platelet-activating systems
    bioRxiv. Immunol. Pub Date : 2020-05-29
    Kavitha Mukund; Kalai Mathee; Shankar Subramaniam

    Recently emerged beta-coronavirus, SARS-CoV-2 has resulted in the current pandemic designated COVID-19. COVID-19 manifests as severe illness exhibiting systemic inflammatory response syndrome, acute respiratory distress syndrome (ARDS), thrombotic events, and shock, exacerbated further by co-morbidities and age. Recent clinical reports suggested that the pulmonary failure seen in COVID-19 may not be

    更新日期:2020-05-29
  • Imaging the mechanisms of anti-CD20 therapy in vivo uncovers spatiotemporal bottlenecks in antibody-dependent phagocytosis
    bioRxiv. Immunol. Pub Date : 2020-05-29
    Capucine L Grandjean; Zacarias Garcia; Fabrice Lemaitre; Beatrice Breart; Philippe Bousso

    Anti-CD20 monoclonal antibody (mAb) represents an effective strategy for the treatment of B cell malignancies that may involve complement activity, antibody dependent cellular cytotoxicity (ADCC) and phagocytosis (ADP). While ADP mediated by Kupffer cells is essential to deplete circulating tumors, the relative contribution of each mechanism to the elimination of non-circulating targets has yet to

    更新日期:2020-05-29
  • Unique maternal immune and functional microbial profiles during prenatal stress
    bioRxiv. Immunol. Pub Date : 2020-05-29
    Adrienne M Antonson; Morgan V Evans; Jeffrey D Galley; Helen J Chen; Therese A Rajesekera; Sydney M Lammers; Vanessa L Hale; Michael T Bailey; Tamar L Gur

    Maternal stress during pregnancy is widespread and stress-induced fetal neuroinflammation is thought to derive from a disruption in intrauterine immune homeostasis, though the exact origins are incompletely defined. We aimed to identify divergent immune and microbial metagenome profiles of stressed gestating mice that may underlie detrimental inflammatory signaling at the maternal-fetal interface.

    更新日期:2020-05-29
  • Peptide:MHC dependent activation of natural killer cells through KIR2DS2 generates anti-tumor responses
    bioRxiv. Immunol. Pub Date : 2020-05-29
    Pauline Rettman; Matthew D Blunt; Berenice Mbiribindi; Rebecca Fulton; Ralf Schittenhelm; Andres F Vallejo; Leidy Bastidas-Legarda; Marta E Polak; Rochelle Ayala; Anthony W E Purcell; Aymen Al-Shamkhani; Christelle Retiere; Salim I Khakoo

    Natural killer (NK) cells are key components of the immune response to viral infections and cancer. Their functions are controlled by activating and inhibitory killer-cell immunoglobulin-like receptors (KIR) which have MHC class I ligands. KIR2DS2 is an activating KIR, that binds conserved viral peptides in the context of HLA-C and has been associated with protective responses to both cancer and viral

    更新日期:2020-05-29
  • Comprehensive Transcriptomic Analysis of COVID-19 Blood, Lung, and Airway
    bioRxiv. Immunol. Pub Date : 2020-05-28
    Andrea R Daamen; Prathyusha Bachali; Katherine A Owen; Kathryn M Kingsmore; Erika L Hubbard; Adam C Labonte; Robert Robl; Sneha Shrotri; Amrie C Grammer; Peter E Lipsky

    SARS-CoV2 is a previously uncharacterized coronavirus and causative agent of the COVID-19 pandemic. The host response to SARS-CoV2 has not yet been fully delineated, hampering a precise approach to therapy. To address this, we carried out a comprehensive analysis of gene expression data from the blood, lung, and airway of COVID-19 patients. Our results indicate that COVID-19 pathogenesis is driven

    更新日期:2020-05-28
  • Evidence for anti-viral effects of complete Freunds adjuvant in the mouse model of enterovirus infection
    bioRxiv. Immunol. Pub Date : 2020-05-28
    Arunakumar Gangaplara; Chandirasegaran Massilamany; Ninaad Lasrado; David Steffen; Jay Reddy

    Group B Coxsackieviruses belonging to the genus, Enterovirus, contain six serotypes that induce various diseases, whose occurrence may involve the mediation of more than one serotype. We recently identified immunogenic epitopes within CVB3 viral protein 1 that induce antiviral T cell responses in mouse models of CVB infections. In our investigations to determine the protective responses of the viral

    更新日期:2020-05-28
  • Single-dose replicating RNA vaccine induces neutralizing antibodies against SARS-CoV-2 in nonhuman primates
    bioRxiv. Immunol. Pub Date : 2020-05-28
    Jesse H Erasmus; Amit P Khandhar; Alexandra C Walls; Emily A Hemann; Megan A O'Connor; Patience Murapa; Jacob Archer; Shanna Leventhal; Jim Fuller; Thomas Lewis; Kevin E Draves; Samantha Randall; Kathryn A Guerriero; Malcolm S Duthie; Darrick Carter; Steven G Reed; David W Hawman; Heinz Feldmann; Michael Gale; David Veesler; Peter Berglund; Deborah Heydenburg Fuller

    The ongoing COVID-19 pandemic, caused by infection with SARS-CoV-2, is having a dramatic and deleterious impact on health services and the global economy. Grim public health statistics highlight the need for vaccines that can rapidly confer protection after a single dose and be manufactured using components suitable for scale-up and efficient distribution. In response, we have rapidly developed repRNA-CoV2S

    更新日期:2020-05-28
  • The antidepressant sertraline provides a novel host directed therapy module for augmenting TB therapy
    bioRxiv. Immunol. Pub Date : 2020-05-28
    Deepthi Shankaran; Anjali Singh; Stanzin Dawa; Prabhakar Arumugam; Sheetal Gandotra; Vivek Rao

    A prolonged therapy regimen, primarily responsible for development of drug resistance by Mycobacterium tuberculosis (Mtb), the causative agent of human TB, obligates any new regimen to not only reduce treatment duration but also escape pathogen resistance mechanisms. With the aim of harnessing the host response in providing additional support to existing regimens (host directed therapy- HDT), we established

    更新日期:2020-05-28
  • Atypical B cells are a normal component of immune responses to vaccination and infection in humans
    bioRxiv. Immunol. Pub Date : 2020-05-28
    Henry J. Sutton; Racheal Aye; Azza H. Idris; Rachel Vistein; Eunice Nduati; Oscar Kai; Jedidia Mwacharo; Li Xi; Xin Gao; Dan Andrews; Marios Koutsakos; Thi H.O. Nguyen; Maxim Nekrasov; Peter Milburn; Auda Ethala; Andrea A. Berry; Natasha KC; Sumana Chakravarty; B. Kim Lee Sim; Adam K. Wheatley; Stephen J. Kent; Stephen L. Hoffman; Kirsten E. Lyke; Philip Bejon; Fabio Luciani; Katherine Kedzierska;

    The full diversity of the circulating human B cell compartment is unknown. Flow cytometry analysis suggests that in addition to naive and memory B cells, there exists a population of CD11c+, CD27- CD21- atypical B cells, that are associated with chronic or recurrent infection and autoimmunity. We used single cell RNA-seq approaches to examine the diversity of both antigen-specific B cells and total

    更新日期:2020-05-28
  • Neutrophils Mediate Kidney Inflammation Following Acute Skin Exposure to UVB Light
    bioRxiv. Immunol. Pub Date : 2020-05-28
    Sladjana Skopelja-Gardner; Joyce Tai; Xizhang Sun; Lena Tanaka; James A Kuchenbecker; Jessica M Snyder; Paul Kubes; Tomas Mustelin; Keith B Elkon

    Photosensitivity to ultraviolet (UV) light affects up to ~80% of lupus patients and can exacerbate local skin disease as well as systemic disease, including lupus nephritis. While neutrophils have been implicated in local tissue injury in lupus in response to immune complex deposition, whether and how they play a role in photosensitivity induced systemic disease is unknown. Here, we show that following

    更新日期:2020-05-28
  • Human IgG neutralizing monoclonal antibodies block SARS-CoV-2 infection
    bioRxiv. Immunol. Pub Date : 2020-05-28
    Jinkai Wan; Shenghui Xing; Longfei Ding; Yongheng Wang; Dandan Zhu; Bowen Rong; Siqing Wang; Kun Chen; Chenxi He; Songhua Yuan; Chengli Qiu; Chen Zhao; Xiaoyan Zhang; Xiangxi Wang; Yanan Lu; Jianqing Xu; Fei Lan

    The coronavirus induced disease 19 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a worldwide threat to human lives, and neutralizing antibodies present a great therapeutic potential in curing affected patients. We purified more than one thousand memory B cells specific to SARS-CoV-2 S1 or RBD (receptor binding domain) antigens from 11 convalescent

    更新日期:2020-05-28
  • Adaptive modification of lipid droplets mediated by Plin1 functions in infection-induced pathogenesis in Drosophila
    bioRxiv. Immunol. Pub Date : 2020-05-28
    Lei Wang; Jiaxin Lin; Junjing Yu; Zhiqin Fan; Hong Tang; Lei Pan

    Lipid droplets (LDs) are dynamic intracellular organelles critical for lipid metabolism. Alterations in the dynamics and functions of LDs during innate immune response to infections and the underlying mechanisms however, remain largely unknown. Herein, we describe the morphological dynamics of LDs in fat body of Drosophila, which vary between transient and sustained bacterial infections. Detailed analysis

    更新日期:2020-05-28
  • Angiotensin-converting enzyme 2, a SARS-CoV-2 receptor, is upregulated by interleukin-6 via STAT3 signaling in rheumatoid synovium
    bioRxiv. Immunol. Pub Date : 2020-05-27
    Sho Mokuda; Tadahiro Tokunaga; Junya Masumoto; Eiji Sugiyama

    Detected in December 2019, the coronavirus disease 2019 (COVID-19) has since spread all over the world, resulting in a global pandemic. The disease is caused by severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), and its symptoms usually include cough, fever, and gastrointestinal problems. Although the prevalence of rheumatoid arthritis (RA) is about 1 % of the global population and RA patients

    更新日期:2020-05-27
  • Development and validation of IMMUNO-COV: a high-throughput clinical assay for detecting antibodies that neutralize SARS-CoV-2
    bioRxiv. Immunol. Pub Date : 2020-05-27
    Rianna Vandergaast; Timothy Carey; Samantha Reiter; Patrycja Lech; Clement Gnanadurai; Mulu Tesfay; Jason Buehler; Lukkana Suksanpaisan; Shruthi Naik; Bethany Brunton; Jordan Recker; Michelle Haselton; Christopher Ziegler; Anne Roesler; John R. Mills; Elitza Theel; Scott C. Weaver; Grace Rafael; Matthew M. Roforth; Clavin Jerde; Sheryl Tran; Rosa Maria Diaz; Alice Bexon; Alina Baum; Christos A. Kyratsous;

    We here describe the development and validation of IMMUNO-COV, a high-throughput clinical test to quantitatively measure SARS-CoV-2-neutralizing antibodies, the specific subset of anti-SARS-CoV-2 antibodies that block viral infection. The test measures the capacity of serum or purified antibodies to neutralize a recombinant Vesicular Stomatitis Virus (VSV) encoding the SARS-CoV-2 spike glycoprotein

    更新日期:2020-05-27
  • Different pattern of pre-existing SARS-COV-2 specific T cell immunity in SARS-recovered and uninfected individuals
    bioRxiv. Immunol. Pub Date : 2020-05-27
    Nina Le Bert; Anthony Tanoto Tan; Kamini Kunasegaran; Christine Y. L. Tham; Morteza Hafezi; Adeline Chia; Melissa Chng; Meiyin Lin; Nicole Tan; Martin Linster; Wan Ni Chia; Mark I-Cheng Chen; Lin-Fa Wang; Eng Eong Ooi; Shirin Kalimuddin; Paul Anantharajal Tambyah; Jenny Guek-Hong Low; Yee-Joo Tan; Antonio Bertoletti

    Memory T cells induced by previous infections can influence the course of new viral infections. Little is known about the pattern of SARS-CoV-2 specific pre-existing memory T cells in human. Here, we first studied T cell responses to structural (nucleocapsid protein, NP) and non-structural (NSP-7 and NSP13 of ORF1) regions of SARS-CoV-2 in convalescent from COVID-19 (n=24). In all of them we demonstrated

    更新日期:2020-05-27
  • Mouse model of SARS-CoV-2 reveals inflammatory role of type I interferon signaling
    bioRxiv. Immunol. Pub Date : 2020-05-27
    Benjamin Goldman-Israelow; Eric Song; Tianyang Mao; Peiwen Lu; Amit Meir; Feimei Liu; Mia Madel Alfajaro; Jin Wei; Huiping Dong; Robert Homer; Aaron Ring; Craig B Wilen; Akiko Iwasaki

    Severe Acute Respiratory Syndrome- Coronavirus 2 (SARS-Cov-2) has caused over 5,000,000 cases of Coronavirus disease (COVID-19) with significant fatality rate. Due to the urgency of this global pandemic, numerous therapeutic and vaccine trials have begun without customary safety and efficacy studies. Laboratory mice have been the stalwart of these types of studies; however, they do not support infection

    更新日期:2020-05-27
  • Tolerance induction in memory CD4 T cells is partial and reversible
    bioRxiv. Immunol. Pub Date : 2020-05-27
    Joshua I Gray; Shaima Al Khabouri; Fraser Morton; Eric Clambey; Laurent Gapin; Jennifer L Matsuda; John W Kappler; Phillipa Marrack; Paul Garside; Thomas D Otto; Megan KL MacLeod

    Memory T cells respond rapidly in part because they are less reliant on heightened levels of costimulatory molecules. This presents challenges to silencing memory T cells in tolerance strategies for autoimmunity or allergy. We find that memory CD4 T cells generated by infection or immunisation survive secondary activation with antigen delivered without adjuvant, regardless of their location in secondary

    更新日期:2020-05-27
  • Construction of Nanobody Library in Mammalian Cells by Linear-double-stranded DNA Based AND Gate Genetic Circuit
    bioRxiv. Immunol. Pub Date : 2020-05-27
    Yanjie Zhao; Xin Tong; Chunze Zhang; Shuai Li

    Nanobody is one special type of single-domain antibody fragment with multiple advantages over traditional antibody. Our previous work established linear-double-stranded DNA (ldsDNA, or PCR amplicon) as novel biological parts for building AND gate genetic circuits in mammalian cells. During this AND-gate circuit formation process, the co-transfected up- and down-stream ldsDNAs could be linked together

    更新日期:2020-05-27
  • TLR9-activated B cells improve their regulatory function by endogenously produced catecholamines
    bioRxiv. Immunol. Pub Date : 2020-05-27
    Nadine Honke; Torsten Lowin; Birgit Opgenoorth; Namir Shaabani; Alexander Lautwein; John R. Teijaro; Matthias Schneider; Georg Pongratz

    The sympathetic nervous system (SNS) contributes to immune balance by promoting anti-inflammatory B cells. However, whether B cells possess a self-regulating mechanism by which they modulate regulatory B cell (Breg) function is not well understood. In this study, we investigated the ability of B cells to synthesize catecholamines upon stimulation with different B cell activators. We found, that expression

    更新日期:2020-05-27
  • PYK2 controls intestinal inflammation via activation of IRF5 in macrophages
    bioRxiv. Immunol. Pub Date : 2020-05-27
    Grigory Ryzhakov; Hannah Almuttaqi; Alastair L Corbin; Tariq Khoyratty; Dorothee Berthold; Samuel Bullers; Hayley L Eames; Zhichao Ai; Sarah Bonham; Roman Fischer; Luke Jostins-Dean; Simon P.L. Travis; Benedikt M Kessler; Irina A Udalova

    Inflammatory bowel disease (IBD) is a group of inflammatory disorders of the gastro-intestinal tract caused by a complex combination of genetic and environmental factors. Interferon regulating factor 5 (IRF5) is a multifunctional regulator of immune responses, which plays a key pathogenic role in mouse colitis models and is a genetic risk factor for IBD. A screen of a protein kinase inhibitor library

    更新日期:2020-05-27
  • Immunomodulatory effects of extract of Ganoderma lucidum basidiocarps cultivated on alternative substrate
    bioRxiv. Immunol. Pub Date : 2020-05-26
    Biljana Božić Nedeljković; Jasmina Cilerdzić; Dragana Zmijanjac; Milan Markovic; Tanja Dzopalic; Sasa Vasilijic; Mirjana Stajic; Dragana Vucevic

    The aim of the study was to investigate if there are any differences in effects of extracts of commercially (GC) and alternatively (wheat straw) (GA) cultivated Ganoderma lucidum basidiocarps on properties of peritoneal macrophages (PM) and monocyte-derived dendritic cells (MoDCs). GA extract stronger stimulated the metabolic and phagocytic activity of PMs, their adhesion capability and ability to

    更新日期:2020-05-26
  • A Vaccine Composed of Antigen Fused to a Ligand for a Receptor on Immature Dendritic Cells Elicits Elevated Antibody Responses to Malaria Sporozoites in Infant Macaques
    bioRxiv. Immunol. Pub Date : 2020-05-26
    Richard B. Markham; Kun Luo; James Gordy; Fidel Zavala

    Infants and young children are the groups at greatest risk for severe disease resulting from Plasmodium falciparum infection. We previously demonstrated in mice that a protein vaccine composed of the chemokine macrophage inflammatory protein 3α genetically fused to the minimally truncated circumsporozoite protein of P. falciparum (MCSP) elicits high concentrations of specific antibody and significant

    更新日期:2020-05-26
  • Identification and physiological significance of temporal NFκB signaling codewords deployed by macrophages to classify immune threats
    bioRxiv. Immunol. Pub Date : 2020-05-26
    Brooks Taylor; Adewunmi Adelaja; Yi Liu; Stefanie Luecke; Alexander Hoffmann

    Acute and chronic inflammatory pathologies involve misregulation of macrophage functions. Physiologically, macrophages are immune sentinels that initiate inflammatory responses via the transcription factor NFκB. The temporal pattern of NFκB activity determines which genes are expressed, suggesting that a temporal signaling code specifies a stimulus-appropriate immune response. To identify the signaling

    更新日期:2020-05-26
  • T-cell hyperactivation and paralysis in severe COVID-19 infection revealed by single-cell analysis
    bioRxiv. Immunol. Pub Date : 2020-05-26
    Bahire Kalfaoglu; José Almeida-Santos; Chanidapa Adele Tye; Yorifumi Satou; Masahiro Ono

    Severe COVID-19 patients can show respiratory failure, T-cell reduction, and cytokine release syndrome (CRS), which can be fatal in both young and aged patients and is a major concern of the pandemic. However, the pathogenetic mechanisms of CRS in COVID-19 are poorly understood. Here we show single cell-level mechanisms for T-cell dysregulation in severe SARS-CoV-2 infection, and thereby demonstrate

    更新日期:2020-05-26
  • Jointly modeling prevalence, sensitivity and specificity for optimal sample allocation
    bioRxiv. Immunol. Pub Date : 2020-05-26
    Daniel B Larremore; Bailey K Fosdick; Sam Zhang; Yonatan H Grad

    The design and interpretation of prevalence studies rely on point estimates of the performance characteristics of the diagnostic test used. When the test characteristics are not well defined and a limited number of tests are available, such as during an outbreak of a novel pathogen, tests can be used either for the field study itself or for additional validation to reduce uncertainty in the test characteristics

    更新日期:2020-05-26
  • Severe murine schistosomiasis results from disruption of the modulation of anti-egg CD4 T cell response by immunodominance of a single egg epitope
    bioRxiv. Immunol. Pub Date : 2020-05-26
    Eduardo Finger; Alessandra Finardi Dastoli; Thaissa Melo Galante Coimbra

    In murine schistosomiasis, previous studies show that failure to modulate the anti-egg CD4 T helper to a Th2-type response, correlates with the exacerbated immunodominance of the major egg antigen Sm-p40 234-246 and the development of severe liver immunopathology. To better understand this connection, we developed a strategy to adjust the level of the immunodominance and test its effect on the severity

    更新日期:2020-05-26
  • Machine learning analysis of the bleomycin-mouse model reveals the spatial and temporal pulmonary inflammatory fingerprint
    bioRxiv. Immunol. Pub Date : 2020-05-26
    Natalie Bordag; Valentina Biasin; Diana Schnoegl; Valzano Francesco; Katharina Jandl; Bence M Nagy; Neha Sharma; Malgorzata Wygrecka; Grazyna Kwapiszewska; Leigh M Marsh

    The bleomycin mouse model is the most extensively used animal model to study pulmonary fibrosis. Despite this, the inflammatory cell kinetics and cell compartmentalisation is still incompletely understood. Here we simultaneously analysed 16 inflammatory cell populations in 303 samples and applied advanced data modelling to conclusively detail these kinetics. Three days post-bleomycin, the inflammatory

    更新日期:2020-05-26
  • Long term sterile immunity induced by an adjuvant-containing live-attenuated AIDS virus
    bioRxiv. Immunol. Pub Date : 2020-05-26
    Tomotaka Okamura; Yuya Shimizu; Tomohiro Kanuma; Yusuke Tsujimura; Masamitsu N Asaka; Kazuhiro Matsuo; Takuya Yamamoto; Yasuhiro Yasutomi

    Antigen 85B (Ag85B) is one of the most dominant proteins secreted from most mycobacterial species, and it induces Th1-type immune responses as an adjuvant. We genetically constructed a live attenuated simian human immunodeficiency virus to express the adjuvant molecule Ag85B (SHIV-Ag85B). SHIV-Ag85B could not be detected 4 weeks after injection in cynomolgus macaques, and strong SHIV-specific T cell

    更新日期:2020-05-26
  • NK cells and CTLs are required to clear solid tumor in a novel model of patient-derived-xenograft
    bioRxiv. Immunol. Pub Date : 2020-05-26
    Duy Tri Le; Bryan Burt; George Van Buren; Shawn A Abeynaike; Cristina Zalfa; Rana Nikzad; Farrah Kheradmand; Silke Paust

    Existing patient-derived-xenograft (PDX) mouse models of solid tumors lack a fully tumor-donor matched 'syngeneic' and functional immune system. We developed such a model by engrafting lymphopenic recipient mice with a fresh undisrupted piece of solid tumor, whereby tumor-infiltrating lymphocytes (TIL) expanded in the recipient mice for several weeks. Tumors engrafted in about seventy to eighty percent

    更新日期:2020-05-26
  • Evidence for prescribed NK cell Ly49 developmental pathways in mice
    bioRxiv. Immunol. Pub Date : 2020-05-26
    Alberto J. Millan; Bryan A. Hom; Jeremy B. Libang; Suzanne Sindi; Jennifer O. Manilay

    Previous studies of NK cell inhibitory Ly49 receptors suggested their expression is stochastic. However, relatively few studies have examined this stochasticity in conjunction with activating Ly49 receptors. We hypothesized that the expression of activating Ly49 receptors is not stochastic and is influenced by inhibitory Ly49 receptors. We analyzed NK cell clusters defined by combinatorial expression

    更新日期:2020-05-26
  • Antibody repertoire and gene expression dynamics of diverse human B cell states during affinity maturation.
    bioRxiv. Immunol. Pub Date : 2020-05-26
    Hamish W King; Nara Orban; John C Riches; Andrew J Clear; Gary Warnes; Sarah A Teichmann; Louisa K James

    In response to antigen challenge, B cells clonally expand, undergo selection and differentiate to produce mature B cell subsets and high affinity antibodies. However, the interplay between dynamic B cell states and their antibody-based selection is challenging to decipher in primary human tissue. We have applied an integrated analysis of bulk and single-cell antibody repertoires paired with single-cell

    更新日期:2020-05-26
  • Regulatory T cells control the dynamic and site-specific polarization of CD4 T cells following Salmonella infection
    bioRxiv. Immunol. Pub Date : 2020-05-25
    Slater L Clay; Alberto Bravo-Blas; Daniel M Wall; Megan K L MacLeod; Simon W F Milling

    FoxP3+ regulatory T cells (Tregs) control inflammation and maintain mucosal homeostasis, but their functions during infection are poorly understood. Th1, Th2 and Th17 cells can be identified by master transcription factors (TFs) T-bet, GATA3 and RORγT; Tregs also express these TFs. While T-bet+ Tregs can selectively suppress Th1 cells, it is unclear whether distinct Treg populations can alter Th bias

    更新日期:2020-05-25
  • Quantitative modeling of the effect of antigen dosage on B-cell affinity distributions in maturating germinal centers
    bioRxiv. Immunol. Pub Date : 2020-05-25
    Marco Molari; Klaus Eyer; Jean Baudry; Simona Cocco; Remi Monasson

    Affinity maturation is a complex dynamical process allowing the immune system to generate antibodies capable of recognizing antigens. We introduce a model for the evolution of the distribution of affinities across the antibody population in germinal centers. The model is amenable to detailed mathematical analysis, and gives insight on the mechanisms through which antigen availability controls the rate

    更新日期:2020-05-25
  • Dectin-1 Molecular Aggregation and Signaling is Sensitive to β-Glucan Structure and Glucan Exposure on Candida albicans Cell Walls
    bioRxiv. Immunol. Pub Date : 2020-05-25
    Eduardo U Anaya; Akram Etemadi Amin; Michael J Wester; Mike Danielson; Kyle Michel; Aaron K Neumann

    Dectin-1A is a C-type Lectin innate immunoreceptor that recognizes β-(1,3:1,6)-glucan, a structural component of Candidaspecies cell walls. The higher order structure of β-glucans ranges from random coil to insoluble fiber due to varying degrees of tertiary (helical) and quaternary structure. Model Saccharomyces cerevisiaeβ-glucans of medium and high molecular weight (MMW and HMW, respectively) are

    更新日期:2020-05-25
  • INeo-Epp: A novel T-cell HLA class-I immunogenicity or neoantigenic epitope prediction method based on sequence related amino acid features
    bioRxiv. Immunol. Pub Date : 2020-05-25
    guangzhi wang; huihui wan; xingxing jian; jian ouyang; yuyu li; xiaoxiu tan; yong zhao; yong lin; Lu Xie

    In silico T-cell epitope prediction plays an important role in immunization experimental design and vaccine preparation. Currently, most epitope prediction research focuses on peptide processing and presentation, e.g. proteasomal cleavage, transporter associated with antigen processing (TAP) and major histocompatibility complex (MHC) combination. To date, however, the mechanism for immunogenicity of

    更新日期:2020-05-25
  • Targeting a Long Non-Coding RNA splicing by novel candidate drug ABX464 to produce the anti-inflammatory microRNA miR-124
    bioRxiv. Immunol. Pub Date : 2020-05-25
    Laurent Manchon; Audrey Vautrin; Aude Garcel; Noelie Campos; Jamal Tazi

    Many nascent long non-coding RNAs have received considerable attention in recent years because of their major regulatory roles in gene expression and signaling pathways at various levels. Indeed long non-coding RNAs undergo the same maturation steps as pre-mRNAs of protein- coding genes, but they are less efficiently spliced and polyadenylated in comparison to them. Here we focus on a specific human

    更新日期:2020-05-25
  • Pro-inflammatory response of non-inflammatory Classical monocytes stimulated with LPS in vitro.
    bioRxiv. Immunol. Pub Date : 2020-05-25
    Claudio Antonio Karsulovic; Fabian Tempio; Mercedez Lopez; Julia Guerrero; Annelise Goecke

    BACKGROUND: CD14 (Monocyte identifying Toll-Like Receptor) and CD16 (FcyRIII co-receptor, marker of inflammatory monocytes) were used to define 3 subpopulations of circulating monocytes with different attributes in terms of inflammatory and phagocytic capabilities. There are contradictory reports regarding response of circulating monocytes to pro-inflammatory or non-inflammatory stimuli in vitro. Here

    更新日期:2020-05-25
  • Immunoglobulin T genes in Neopterygii
    bioRxiv. Immunol. Pub Date : 2020-05-24
    Serafin Mirete-Bachiller; David N Olivieri; Francisco Gambon-Deza

    In teleost fishes there are three immunoglobulin isotypes named immunoglobulin M (IgM), D (IgD) and T (IgT). IgT has been the last to be described and is considered a teleosts-fish specific isotype. From the recent availability of genome sequences of fishes, an in-depth analysis of Actinopterygii immunoglobulin heavy chain genes was undertaken. With the aid of a bioinformatics pipeline, a machine learning

    更新日期:2020-05-24
  • Deep immune profiling of COVID-19 patients reveals patient heterogeneity and distinct immunotypes with implications for therapeutic interventions
    bioRxiv. Immunol. Pub Date : 2020-05-23
    Divij Mathew; Josephine R Giles; Amy E Baxter; Allison R Greenplate; Jennifer E Wu; Cecile Alanio; Derek A Oldridge; Leticia Kuri-Cervantes; M Betina Pampena; Kurt D'Andrea; Sasikanth Manne; Zeyu Chen; Yinghui Jane Huang; John P Reilly; Ariel R Weisman; Caroline A.G. Ittner; Oliva Kuthuru; Jeanette Dougherty; Kito Nzingha; Nicholas Han; Justin Kim; Ajinkya Pattekar; Eileen C Goodwin; Elizabeth M Anderson;

    COVID-19 has become a global pandemic. Immune dysregulation has been implicated, but immune responses remain poorly understood. We analyzed 71 COVID-19 patients compared to recovered and healthy subjects using high dimensional cytometry. Integrated analysis of ~200 immune and >30 clinical features revealed activation of T cell and B cell subsets, but only in some patients. A subgroup of patients had

    更新日期:2020-05-23
  • Intestinal inflammation modulates the expression of ACE2 and TMPRSS2 and potentially overlaps with the pathogenesis of SARS-CoV-2 related disease
    bioRxiv. Immunol. Pub Date : 2020-05-23
    Mayte Suárez-Fariñas; Minami Tokuyama; Gabrielle Wei; Ruiqi Huang; Alexandra Livanos; Divya Jha; Anais Levescot; Roman Kosoy; Haritz Irizar; Sascha Cording; Wenhui Wang; Ryan Ungaro; Antonio Di Narzo; Gustavo Martinez; Maria Suprun; Michael J Corley; Aleksandar Stojmirovic; Sander Houten; Mark Curran; Carrie Brodmerkel; Jacqueline Perrigoue; Joshua R Friedman; Ke Hao; Eric E Schadt; Jun Zhu; Huaibin

    Immune dysregulation and cytokine release syndrome have emerged as pathological hallmarks of severe Coronavirus Disease 2019 (COVID-19), leading to the evaluation of cytokine antagonists as therapeutic agents. A number of immune-directed therapies being considered for COVID-19 patients are already in clinical use in chronic inflammatory conditions like inflammatory bowel disease (IBD). These considerations

    更新日期:2020-05-23
  • T cells Mediate Progression of Load-Induced Osteoarthritis
    bioRxiv. Immunol. Pub Date : 2020-05-23
    Tibra A. Wheeler; Adrien Y. Antoinette; Matthew J. Kim; Marjolein C.H. van der Meulen; Ankur Singh

    Osteoarthritis (OA) is a degenerative disease that manifests as joint damage and synovial inflammation. To date, most studies have focused on the decrease in cartilage stiffness, chondrocyte viability, and changes in matrix-degrading enzymes. With the exception of a few inflammatory cytokines and macrophages, the immune response in OA is poorly characterized, and the crosstalk of joint damage with

    更新日期:2020-05-23
  • Systemic and mucosal antibody secretion specific to SARS-CoV-2 during mild versus severe COVID-19
    bioRxiv. Immunol. Pub Date : 2020-05-23
    Carlo Cervia; Jakob Nilsson; Yves Zurbuchen; Alan Valaperti; Jens Schreiner; Aline Wolfensberger; Miro E. Raeber; Sarah Adamo; Marc Emmenegger; Sara Hasler; Philipp P. Bosshard; Elena De Cecco; Esther Baechli; Alain Rudiger; Melina Stuessi-Helbling; Lars C. Huber; Annelies S. Zinkernagel; Dominik J. Schaer; Adriano Aguzzi; Ulrike Held; Elsbeth Probst-Mueller; Silvana K. Rampini; Onur Boyman

    Background: Infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes an acute illness termed coronavirus disease 2019 (COVID-19). Humoral immune responses likely play an important role in containing SARS-CoV-2, however, the determinants of SARS-CoV-2-specific antibody responses are unclear. Methods: Using immunoassays specific for the SARS-CoV-2 spike protein, we determined

    更新日期:2020-05-23
  • Lipopolysaccharide preconditioning augments phagocytosis of malaria-parasitized red blood cells through induced bone marrow-derived macrophages in the liver, thereby increasing the murine survival after Plasmodium yoelii infection
    bioRxiv. Immunol. Pub Date : 2020-05-23
    Takeshi Ono; Yoko Yamaguchi; Hiroyuki Nakashima; Masahiro Nakashima; Takuya Ishikiriyama; Shuhji Seki; Manabu Kinoshita

    Malaria remains a grave concern for humans, as effective medical countermeasures for malaria infection have yet to be developed. Phagocytic clearance of parasitized red blood cells (pRBCs) by macrophages is an important front-line innate host defense against malaria infection. We previously showed that repeated injections of low-dose lipopolysaccharide (LPS) prior to bacterial infection, called LPS

    更新日期:2020-05-23
  • Intrinsic defects in lymph node stromal cells underpin poor germinal center responses during aging
    bioRxiv. Immunol. Pub Date : 2020-05-23
    Alice E Denton; Alyssa Silva-Cayetano; James Dooley; Danika L Hill; Edward J Carr; Philippe A Robert; Michael Meyer-Hermann; Adrian Liston; Michelle A Linterman

    The failure to generate enduring humoral immunity after vaccination is a hallmark of advancing age. This can be attributed to a reduction in the germinal center response, which generates long-lived antibody-secreting cells that provide protection against (re)infection. Despite intensive investigation into the effect of age on the lymphoid compartment, the primary cellular defect that causes impaired

    更新日期:2020-05-23
  • PD-L1 expression in equine malignant melanoma and functional effects of PD-L1 blockade
    bioRxiv. Immunol. Pub Date : 2020-05-22
    Otgontuya Ganbaatar; Satoru Konnai; Tomohiro Okagawa; Yutaro Nojima; Naoya Maekawa; Erina Minato; Atsushi Kobayashi; Ryo Ando; Nobuya Sasaki; Daisuke Miyakosi; Osamu Ichi; Yukinari Kato; Yasuhiko Suzuki; Shiro Murata; Kazuhiko Ohashi

    Abstract Programmed death-1 (PD-1) is an immunoinhibitory receptor expressed on exhausted T cells during chronic illness. Interaction of PD-1 with its ligand PD-ligand 1 (PD-L1) delivers inhibitory signals and impairs proliferation, cytokine production, and cytotoxicity of T cells. We reported that the PD-1/PD-L1 pathway is closely associated with T-cell exhaustion and disease progression in bovine

    更新日期:2020-05-22
  • A modular framework for the development of targeted Covid-19 blood transcript profiling panels
    bioRxiv. Immunol. Pub Date : 2020-05-22
    Darawan Rinchai; Basirudeen Kabeer; Mohammed Toufiq; Zohreh Calderone; Sara Deola; Tobias Brummaier; Mathieu Garand; Ricardo Branco; Nicole Baldwin; Mohamed Alfaki; Matthew Altman; Alberto Ballestrero; Matteo Bassetti; Gabriele Zoppoli; Andrea De Maria; Benjamin Tang; Davide Bedognetti; Damien Chaussabel

    Covid-19 morbidity and mortality are associated with a dysregulated immune response. Tools are needed to enhance existing immune profiling capabilities in affected patients. Here we aimed to develop an approach to support the design of focused blood transcriptome panels for profiling the immune response to SARS-CoV-2 infection. We designed a pool of candidates based on a pre-existing and well-characterized

    更新日期:2020-05-22
  • Potently neutralizing human antibodies that block SARS-CoV-2 receptor binding and protect animals
    bioRxiv. Immunol. Pub Date : 2020-05-22
    Seth J Zost; Pavlo Gilchuk; James Brett Case; Elad Binshtein; Rita E Chen; Joseph X Reidy; Andrew Trivette; Rachel S Nargi; Rachel E Sutton; Naveen Suryadevara; Lauren E Williamson; Elaine C Chen; Taylor Jones; Samuel Day; Luke Myers; Ahmed O Hassan; Natasha M Kafai; Emma S Winkler; Julie M Fox; James J Steinhardt; Kuishu Ren; Yueh-Ming Loo; Nicole L Kallewaard; David R Martinez; Alexandra Schafer;

    The COVID-19 pandemic is a major threat to global health for which there are only limited medical countermeasures, and we lack a thorough understanding of mechanisms of humoral immunity. From a panel of monoclonal antibodies (mAbs) targeting the spike (S) glycoprotein isolated from the B cells of infected subjects, we identified several mAbs that exhibited potent neutralizing activity with IC50 values

    更新日期:2020-05-22
  • Perforin-2 limits pathogen proliferation at the maternal-fetal interface
    bioRxiv. Immunol. Pub Date : 2020-05-22
    Petoria Gayle; Vanessa McGaughey; Rosmely Hernandez; Ka Lam Nguyen; Marshall Arons; Laura Padula; Natasa Strbo; Marina Wylie; Rachel C Colletti; Kurt Schesser

    Placental immune responses are highly regulated to strike a balance between protection and tolerance. For relatively mild infections, protection encompasses both the mother and fetus; however, during worsening conditions, protection becomes exclusively reserved for the mother. Previously, we and others have shown that the host factor Perforin-2 plays a central role in protecting mice and cells against

    更新日期:2020-05-22
  • Antibody recognition of the Pneumovirus fusion protein trimer interface
    bioRxiv. Immunol. Pub Date : 2020-05-22
    Jiachen Huang; Darren Diaz; Jarrod James Mousa

    Human metapneumovirus is a leading cause of viral respiratory infection in children, and can cause severe lower respiratory infection in infants, the elderly, and immunocompromised patients. However, there remain no licensed vaccines or specific treatments for hMPV infection. Although the hMPV fusion (F) protein is the sole target of neutralizing antibodies, the immunological properties of hMPV F are

    更新日期:2020-05-22
  • CD4+ T-cell epitope prediction by combined analysis of antigen conformational flexibility and peptide-MHCII binding affinity
    bioRxiv. Immunol. Pub Date : 2020-05-22
    Tysheena P Charles; Daniel L. Moss; Pawan Bhat; Peyton W Moore; Kummer A Nicholas; Avik Bhattacharya; Ramgopal R. Mettu; Samuel J Landry

    Antigen processing in the class II MHC pathway depends on conventional proteolytic enzymes, potentially acting on antigens in native-like conformational states. CD4+ epitope dominance arises from a competition between antigen folding, proteolysis, and MHCII binding. Protease-sensitive sites, linear antibody epitopes, and CD4+ T-cell epitopes were mapped in the plague vaccine candidate F1-V to evaluate

    更新日期:2020-05-22
  • CD45 pre-exclusion from the tips of microvilli establishes a phosphatase-free zone for early TCR triggering
    bioRxiv. Immunol. Pub Date : 2020-05-22
    Yunmin Jung; Lai Wen; Amnon Altman; Klaus Ley

    The tyrosine phosphatase CD45 is a major gatekeeper for restraining T cell activation. Its exclusion from the immunological synapse (IS) is crucial for TCR signal transduction. Here, we used expansion super-resolution microscopy to reveal that CD45 is pre-excluded from the tips of microvilli on primary T cells prior to antigen encounter. This pre-exclusion was diminished by depleting cholesterol or

    更新日期:2020-05-22
  • Netome: The Molecular Characterization of Neutrophil Extracellular Traps (NETs)
    bioRxiv. Immunol. Pub Date : 2020-05-22
    David Scieszka; Yi-Han Lin; Weizhong Li; Saibyasachi Choudhury; Yanbao Yu; Marcelo Freire

    Neutrophils are the most abundant type of white blood cells in humans with biological roles relevant to inflammation and fighting infections. The release of neutrophil extracellular DNA aims to control invasion by bacteria, viruses, fungi, and tissue damage. Neutrophil Extracellular Traps (NETs) act as antimicrobial agents triggering immune signaling through the release of the nuclear content into

    更新日期:2020-05-22
  • Yeast-Expressed SARS-CoV Recombinant Receptor-Binding Domain (RBD219-N1) Formulated with Alum Induces Protective Immunity and Reduces Immune Enhancement
    bioRxiv. Immunol. Pub Date : 2020-05-22
    Wen-Hsiang Chen; Xinrong Tao; Bi-Hung Peng; Anurodh Agrawal; Abdullah Algaissi; Jeroen Pollet; Ulrich Strych; Maria Elena Bottazzi; Peter J Hotez; Sara Lustigman; Lanying Du; Shibo Jiang; Chien-Te K Tseng

    We developed a severe acute respiratory syndrome (SARS) subunit recombinant protein vaccine candidate based on a high-yielding, yeast-engineered, receptor-binding domain (RBD219-N1) of the SARS beta-coronavirus (SARS-CoV) spike (S) protein. When formulated with Alhydrogel®, RBD219-N1 induced high-level neutralizing antibodies against both pseudotyped virus and a clinical (mouse-adapted) isolate of

    更新日期:2020-05-22
  • Convergent Antibody Responses to SARS-CoV-2 Infection in Convalescent Individuals
    bioRxiv. Immunol. Pub Date : 2020-05-22
    Davide F. Robbiani; Christian Gaebler; Frauke Muecksch; Julio Cetrulo Lorenzi; Zijun Wang; Alice Cho; Marianna Agudelo; Christopher Barnes; Shlomo Finkin; Thomas Hagglof; Thiago Oliveira; Charlotte Viant; Arlene Hurley; Katrina Millard; Rhonda Kost; Melissa Cipolla; Anna Gazumyan; Kristie Gordon; Filippo Bianchini; Spencer Chen; Victor Ramos; Roshni Patel; Juan Dizon; Irina Shimeliovich; Pilar Mendoza;

    During the COVID-19 pandemic, SARS-CoV-2 infected millions of people and claimed hundreds of thousands of lives. Virus entry into cells depends on the receptor binding domain (RBD) of the SARS-CoV-2 spike protein (S). Although there is no vaccine, it is likely that antibodies will be essential for protection. However, little is known about the human antibody response to SARS-CoV-2. Here we report on

    更新日期:2020-05-22
  • An insertion unique to SARS-CoV-2 exhibits superantigenic character strengthened by recent mutations
    bioRxiv. Immunol. Pub Date : 2020-05-21
    Mary Hongying Cheng; She Zhang; Rebecca A. Porritt; Moshe Arditi; Ivet Bahar

    Multisystem Inflammatory Syndrome in Children (MIS-C) associated with Coronavirus Disease 2019 (COVID-19) is a newly recognized condition in which children with recent SARS-CoV-2 infection present with a constellation of symptoms including hypotension, multiorgan involvement, and elevated inflammatory markers. These symptoms and the associated laboratory values strongly resemble toxic shock syndrome

    更新日期:2020-05-21
  • IgY antibodies against Ebola virus possess post-exposure protection and excellent thermostability
    bioRxiv. Immunol. Pub Date : 2020-05-21
    Limin Yang; Yuan Zhang; Yanqiu Wei; Yunlong Li; Xuan Wang; Yang Liu; Deyu Tian; Xiaojuan Jia; Rui Gong; Wenjun Liu

    Ebola virus (EBOV) is the most virulent pathogens that cause hemorrhagic fever with high mortality rates in humans and nonhuman primates. The postexposure antibody therapies to prevent EBOV infection are considered efficient. However, due to the poor thermal stability of mammalian antibody, their application in the tropics has been limited. Here, we developed a thermostable therapeutic antibody against

    更新日期:2020-05-21
  • Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signaling
    bioRxiv. Immunol. Pub Date : 2020-05-21
    Jasper J Koning; Anusha Rajaraman; Rogier M Reijmers; Tanja Konijn; Junliang Pan; Carl F Ware; Eugene C Butcher; Reina E Mebius

    Specialized stromal cells occupy and help define B- and T cell domains, which is crucial for proper functioning of our immune system. Signaling through lymphotoxin and TNF-receptors is crucial for development of different stromal subsets which are thought to arise from a common precursor. However, mechanisms that control the selective generation of the different stromal phenotypes are not known. Here

    更新日期:2020-05-21
  • Schistosoma mansoni infection reprograms the metabolic potential of the myeloid lineage in a mouse model of metabolic syndrome
    bioRxiv. Immunol. Pub Date : 2020-05-21
    Diana Cortes-Selva; Lisa Gibbs; J. Alan Maschek; Tyler Van Ry; Bartek Rajwa; James E Cox; Eyal Amiel; Keke C Fairfax

    Despite strong evidence that helminth infections are protective against the development of metabolic disease, a major gap exists in understanding the mechanism(s) underlying this. We have previously found that Schistosoma mansoni induces profound alterations to the metabolic transcriptome of hepatic macrophages and protects male ApoE -/- on high fat diet from the development of obesity, glucose intolerance

    更新日期:2020-05-21
  • A defect in COPI-mediated transport of STING causes immune dysregulation in COPA syndrome
    bioRxiv. Immunol. Pub Date : 2020-05-20
    Anthony K Shum; Zimu Deng; Zhenlu Chong; Christopher S Law; Kojiro Mukai; Frances O Ho; Tereza Martinu; Bradley J Backes; Walter L Eckalbar; Tomohiko Taguchi

    Pathogenic COPA variants cause a Mendelian syndrome of immune dysregulation with elevated type I interferon signaling. COPA is a subunit of coat protein complex I (COPI) that mediates Golgi to ER transport. Missense mutations that disrupt the WD40 domain impair binding and sorting of proteins targeted for retrieval to the ER but how this causes disease remains unknown. Given the importance of COPA

    更新日期:2020-05-20
Contents have been reproduced by permission of the publishers.
导出
全部期刊列表>>
智控未来
聚焦商业经济政治法律
跟Nature、Science文章学绘图
控制与机器人
招募海内外科研人才,上自然官网
隐藏1h前已浏览文章
课题组网站
新版X-MOL期刊搜索和高级搜索功能介绍
ACS材料视界
x-mol收录
湖南大学化学化工学院刘松
上海有机所
廖良生
南方科技大学
西湖大学
伊利诺伊大学香槟分校
徐明华
中山大学化学工程与技术学院
试剂库存
天合科研
down
wechat
bug