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Integrative comparison of cadmium and iron oxide as yellow pigment in terms of cellular stress and genotoxicity in vitro and in vivo Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2021-01-18 Hyun Soo Kim, Hyun Jin Lee, Won-Young Jung, Jong-Il Weon, Dong Yeop Shin, Yujin Jang, Young Rok Seo, Preeyaporn Koedrith
Backgrounds Heavy metals cadmium has been used as a yellow pigment due to its bright and lasting color. Considering the toxicity of cadmium, yellow iron oxide has been suggested as a substitute due to its cost-effectiveness. However, cellular and molecular safety information of yellow iron oxide is not fully understood. Methods Metal-mediated cellular stress indicated by metallothionein 1 expression
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Keratinocyte-specific knockout mice models via Cre–loxP recombination system Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-12-18 Ji Won Son, Jung Jin Shin, Min-Gyu Kim, Jaehyung Kim, Sang Wook Son
Purpose of review The Cre recombinase/loxP (Cre/loxP) system has emerged as a useful tool in genetic manipulations. Generally, any DNA sequence of interest can be deleted when flanked with loxP sites. The conditional (timely or spatially controlled) expression of Cre recombinase enables to determine, where (e.g. in which cell type or tissue) and when (at which time of life or of developmental stage
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Development of virtual reality visualization model (VRVM) with relative spatiotemporality for visual contents in molecular toxicology education Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-12-15 Seunghyun Kim, Ryoun Heo, Yeonji Chung, Sunghyo Park, Jung Min Kim, Michelle P. Kwon, Gil-Hong Park, Meyoung-Kon Kim
Background Visualizing educational contents makes learning more efficient and effective especially in the area such as molecular toxicology, which is time consuming and intellectually taxing to learn. Objective A design principle based on cognitive neuroscience was developed for spatiotemporality of information and optimized virtual reality (VR) for molecular toxicology. We modeled VR with the tricarboxylic
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Correction to: Aqueous extract of Phragmites commun is rhizomes attenuates phototoxicity in skin cells Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-12-14 Sung Hyeok Kim, Chang Woo Ha, Hyosun Lim, Sohee Jang, Seung Namkoong, Sungsil Hong, Youn Kyu Kim, Jae-Young Kim, Sung Ryul Lee, Eun-Hwa Sohn
Unfortunately the second affiliation of the author Eun-Hwa Sohn has been omitted.
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MiR-193b enhanced proliferation and migration and inhibits apoptosis through targeting RAB7A in osteosarcoma cell Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-12-14 Yuan-yuan Zhang, Hai-yan Xu, Jing-jing Dai
Background Accumulating study indicated that microRNA (miRNA) played critical role in the osteosarcoma (OS). The role and mechanisms of miR-193b in OS cell lines were still unknown. Objective We resolved the miR-193b expression in OS cell line and normal cell by RT-PCR assay. The effects of upregulated miR-193b on OS cell proliferation, migration, invasion and apoptosis were evaluated using CCK-8 assay
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The pine wood nematode Bursaphelenchus xylophilus and molecular diagnostic methods Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-12-07 Jin-Pyo Lee, Simranjeet Singh Sekhon, Ji Hun Kim, Sun Chang Kim, Byung-Kwan Cho, Ji-Young Ahn, Yang-Hoon Kim
Background Bursaphelenchus xylophilus, commonly known as the pine wood nematode (PWN), is one of the most destructive nematode species. It causes pine wilt, a slow progressive disease that results in economic loss worldwide. Objective Here, we review the nematode detection methods, including morphological, molecular, chemical, and protein-based methods, and assess how the nematode’s distribution, virulence
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Aqueous extract of Phragmites commun is rhizomes attenuates phototoxicity in skin cells Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-11-08 Sung Hyeok Kim, Chang Woo Ha, Hyosun Lim, Sohee Jang, Seung Namkoong, Sungsil Hong, Youn Kyu Kim, Jae-Young Kim, Sung Ryul Lee, Eun-Hwa Sohn
Background Excessive sunlight exposure leads to photodamaged skin, resulting in wrinkles, roughness, relaxation, and pigmentation. We examined photoprotective effects of aqueous extracts of dried Phragmites communis rhizome (PCWE) on ultraviolet B radiation (UVB)-mediated photodamage in skin cells. Methods Human dermal fibroblasts (HDFs), melanocytes (B16F10 cells), and keratinocytes (HaCaT cells)
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N-type Ca v channel inhibition by spider venom peptide of Argiope bruennichi Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-11-06 In-Wook Hwang, Min Kyoung Shin, Yoo-Jung Lee, Seung Tae Kim, Sue Yeon Lee, Byungjo Lee, Wonhee Jang, Joo-Hong Yeo, Seungki Lee, Jung-Suk Sung
Background The spider venom is composed of various bioactive peptides and has well-known physiological characteristics, such as cytolytic and neurotoxic activities. However, there have been few studies on neurotoxic peptides derived from domestic indigenous spiders in Korea. Objective The study aimed to characterize and identify the venom peptide through genomic analysis from the domestic indigenous
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Genetic association between the rs12252 SNP of the interferon-induced transmembrane protein gene and influenza A virus infection in the Korean population Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-11-04 Yong-Chan Kim, Min-Ju Jeong, Byung-Hoon Jeong
Background Interferon-induced transmembrane protein 3 (IFITM3) is a potent host antiviral effector protein that blocks the invasion of various viruses, including the influenza A virus (IAV). The C allele of the rs12252 single nucleotide polymorphism (SNP) shows vulnerability to the pandemic 2009 H1N1 IAV in European and Asian populations. Objective Here, we estimated the disease susceptibility of the
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Dual role of reactive oxygen species in autophagy and apoptosis induced by compound PN in prostate cancer cells Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-11-02 Hyeun Deok Choi, Kwang-Youn Kim, Kwang Il Park, Sang-Hun Kim, Sul-Gi Park, Sun-Nyoung Yu, Young-Woo Kim, Dong Seob Kim, Kyung Tae Chung, Soon-Cheol Ahn
Background Pharbitis nil (L.) Choisy (PN) is used as a traditional herb in East Asia and exhibits anti-parasitic, purgative, diuretic, anti-inflammatory, and anti-cancer activities. However, the molecular mechanisms underlying the anti-cancer activity are not well understood. Objective This study aims to elucidate the effects of reactive oxygen species (ROS), generated after treatment with the compound
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Correction to: The neuroprotective effects of stimulation of NMDA receptors against POX-induced neurotoxicity in hippocampal cultured neurons; a morphometric study Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-10-06 Farideh Bahrami, Zahra Bahari, Reyhaneh Abolghasemi, Lida Golmanesh, Gholam Hossein Meftahi
Due to an unfortunate oversight, one of the author names has been given erroneously.
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Decreased plasma α-synuclein in idiopathic Parkinson’s disease patients after adjusting hemolysis factor Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-09-15 Kyu Hwan Shim, Seung Chan Kim, Young Chul Youn, Young-Hee Sung, Seong Soo A. An
Background Lewy bodies are pathological hallmarks for the diagnosis of Parkinson’s disease, where the core components are composed of aggregated forms of α-synuclein (α-syn). Although α-syn has been investigated as a potential biomarker for PD, its usage has been limited and still remains controversial. Objective An accurate enzyme-linked immunosorbent assay (ELISA) was developed for the detection
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Curcumin promotes oxidative stress, apoptosis and autophagy in H9c2 rat cardiomyoblasts Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-09-11 Iván Zepeda-Quiróz, Helen Sánchez-Barrera, Zaira Colín-Val, Diana Xochiquetzal Robledo-Cadena, Sara Rodríguez-Enríquez, Rebeca López-Marure
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Effects of α-mangostin on embryonic development and liver development in zebrafish Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-09-11 Wittaya Pimtong, Wannakarn Kitipaspallop, Hang-Suk Chun, Woo-Keun Kim
Background Alpha-mangostin has potential as a chemopreventive agent but there is little information on its toxicological profile and developmental toxicity. Objective We evaluated the effects of α-mangostin on embryonic development and hepatogenesis in zebrafish. Result Exposure of embryos to 0.25–4 μM α-mangostin from 4–120 h post-fertilization (hpf) caused mortality of embryos with LC50 1.48 ± 0
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Propofol ameliorated diabetic peripheral neuropathic pain via modulating miR-150/EPHB2 axis Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-09-09 Dongyi Fan, Simin Yang, Jingyu Yao, Yuxiang Han, Lukun Yang
Background Diabetic peripheral neuropathic pain (DPNP) is a kind of common diabetic chronic complications and affects life quality of patients. Given the protective function of propofol in multiple diseases, the aim of this study is to investigate the mechanism of propofol in DPNP. Methods The streptozocin (STZ)-induced DPNP rat models were established for in vivo study and the high glucose (HG)-induced
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Capture and toxicity assessment of Ag citrate nanoparticles using jellyfish extract Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-09-08 Jiwon Ha, Euna Kim, Bong Gu Lee, Min-Kyeong Yeo
Backgrounds In this study, Jellyfish extract at immunity reaction (JEI) captured Ag citrate nanoparticles (Ag citrate NPs) and reduce toxicity was investigated. Methods JEI obtained from Aurelia aurita was used to assess the capture of Ag citrate NPs (10 mg/L, 2 mg/L) during 24 h, and the toxicity of JEI was assessed using a human cell line BEAS-2B. Results JEI was to reduce Ag citrate NPs in water
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Indole-6-carboxaldehyde prevents oxidative stress-induced mitochondrial dysfunction, DNA damage and apoptosis in C2C12 skeletal myoblasts by regulating the ROS-AMPK signaling pathway Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-09-07 Cheol Park, Hyesook Lee, Shin-Hyung Park, Su Hyun Hong, Kyoung Seob Song, Hee-Jae Cha, Gi-Young Kim, Young-Chae Chang, Suhkmann Kim, Heui-Soo Kim, Yung Hyun Choi
Background Indole-6-carboxaldehyde (I6CA), a natural indole derivative derived from the brown algae Sargassum thunbergii (Mertens) Kuntze, is known to have several pharmacological activities. However, the antioxidant effects of I6CA have not been identified. Objective The study aimed to investigate the protective effect of I6CA and its underlying mechanism against oxidative stress-induced damage in
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Exposure to mercury induced early apoptotic signals in human placental BeWo cells through alteration of cell cycle regulation Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-08-11 Mi Jin Kim, Chul-Hong Kim, Mi-Jin An, Geun-Seup Shin, Hyun-Min Lee, Ji-Young Kim, Jae Yoon Hwang, Ju-Hyun Lee, Jung-Woong Kim
Backgrounds Heavy metals are environmental pollutants and their breakdown is regarded as a serious risk to human health. Despite increasing evidence that heavy metals have adverse effects in vivo and in vitro, there is no evidence of the effect of heavy metals during placental formation. Objective We determined the effect of heavy metals on cell viability of BeWo human placental cells using MTS assay
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Methylparaben induces cell-cycle arrest and caspase-3-mediated apoptosis in human placental BeWo cells Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-08-08 Ji-Young Kim, Mi Jin Kim, Mi-Jin An, Geun-Seup Shin, Hyun-Min Lee, Chul-Hong Kim, Jung-Woong Kim
Background Parabens are frequently used as antimicrobial preservatives in various commercials, pharmaceutical products, and foods. Recent studies have suggested that the exposure of pregnant women to methylparaben has adverse effects on the fetus. However, the biological mechanisms underlying the effects of methylparaben on human placental cells are yet to be elucidated. Objective In this study, we
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Additional diagnostic testing of the 2019 novel coronavirus (SARS-CoV-2). Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-07-07 Van Giau Vo,Eva Bagyinszky,Kyuhwan Shim,Yoon Soo Park,Seong Soo A An
Purpose of review Within the last two decades several members of the Coronaviridae family namely Severe Respiratory Syndrome (SARS-CoV) and Middle East Respiratory Syndrome (MERS-CoV) have demonstrated epidemic potential. In late, 2019 an unnamed genetic relative, later named SARS-CoV-2 realized its potential in the highly populous neighborhoods of Wuhan, China. Unchecked, the virus rapidly spread
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Graviola leaves extract enhances the anticancer effect of cisplatin on various cancer cell lines Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-06-29 Mai G. Awad, Ramadan A. Ali, Dalia D. Abd El-Monem, Mohammed A. El-Magd
Background Cisplatin (CIS) is widely applied as an anticancer drug for various cancer types, including liver, breast, colorectal, and pancreatic cancers; however, its usage is limited due to side effects. Objective We investigated whether combined therapy of Graviola (Annona muricata) leaves extract (GLE) and CIS could reduce CIS doses without decreasing its anticancer potential. Methods The MCF7,
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The neuroprotective effects of stimulation of NMDA receptors against POX-induced neurotoxicity in hippocampal cultured neurons; a morphometric study Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-06-29 Farideh Bahrami, Zahra Bahari, Reihaneh Abolghasemi, Lida Golmanesh, Gholam Hossein Meftahi
Background Organophosphorus agents are potent neurotoxins that widely used as insecticides and also as poison in warfare. In the current study, we examined the neuroprotective role of NMDA receptors in Paraoxon (POX)-induced neurotoxicity of hippocampal cultured neurons. Objective Hippocampal neurons were isolated from the brains of neonatal rats. The cells treated with POX (100 µM) for 48 h or NMDA
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Comparative analysis of acute and chronic stress-induced neurobehavioral alteration and liver injury in mice Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-06-26 Tae Woo Oh, Kwang-Youn Kim, Hyun Ju Do, Young-Woo Kim, Kwang-Il Park
Backgrounds Recent research has shown that stress has a significant influence on the functions of many organs. Objective The present study investigated the effect of acute or chronic restraint stress on physiological and psychological processes. Results In the acute stress group, body weight and food intake did not change, but in the chronic stress group, food intake significantly increased and body
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Paclitaxel inhibits proliferation and promotes apoptosis through regulation ROS and endoplasmic reticulum stress in osteosarcoma cell Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-06-26 Ming Li, Liming Yin, Lili Wu, Yunsen Zhu, Xi Wang
Background Paclitaxel is a chemotherapeutic drug for cancer, which is isolated from the Pacific yew tree. However, and the molecular mechanism and the antitumor effects of paclitaxel on osteosarcoma cell remain to be explored. Objective The aim of our study was to explore the possible molecular mechanisms of apoptosis in osteosarcoma induced by paclitaxel. Results Paclitaxel can obviously decrease
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lncRNA NONHSAT021963, which upregulates VEGF in A549 cells, mediates PM2.5 exposure-induced angiogenesis in Shenyang, China Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-06-24 Biao Yang, Han Tian, Chunling Xiao
Background PM2.5 is airborne particulate matter that is involved in air pollution and has become an important problem endangering human health. LncRNAs play important roles in malignant tumors. However, few studies have reported the mechanisms by which PM2.5 affects lung cancer. Objective To investigate the functional changes in PM2.5-exposed cells, we conducted tubule formation assays to determine
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HS-1793 protects C2C12 cells from oxidative stress via mitochondrial function regulation Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-06-17 Jubert Marquez, Nammi Park, Maria Victoria Faith Garcia, Hyoung Kyu Kim, Jin Han
Background HS1793, a novel analogue of resveratrol, was previously determined to be more potent at lower dosages by improving mitochondrial function and increased mitochondrial biogenesis-related proteins. In this study, we focused on targeting the mitochondria to address muscle wasting with HS-1793. Method Dosage screening was performed by evaluating for cytotoxicity and cell proliferation. Mitochondrial
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Toxic effect of titanium dioxide nanoparticles on human mesenchymal stem cells Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-06-10 Peralta-Vega Adriana-Berenice, Parra-Barrera Alberto, Ramos-Godínez María del Pilar, López-Marure Rebeca, Arellano-Galindo José, Gisela Gutiérrez-Iglesias
Background Titanium dioxide (TiO2) is employed in the production of cosmetics, sunscreen, food, and drugs; however, TiO2 is toxic at the nanometric scale. Objective To analyze the in vitro toxic effect of TiO2 NPs on mesenchymal stem cells (hMSCs). Methods The hMSCs from Wharton`s jelly were exposed to 4, 62.5 and 500 μg/mL of TiO2 NPs. Viability and cell proliferation tests were carried out at 2,
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Myricetin induces apoptosis mediated by oxidative stress in 4T1 and E0771 mammary cancer cells Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-06-08 Allison Knickle, Andrea Rasmussen, David W. Hoskin
Background Myricetin is a polyphenolic compound that is cytostatic and/or cytotoxic for several cancer cell types; however, little is known about its effect on mammary carcinoma cells. Objective The objective of this study is to determine whether myricetin affects the growth of mammarycarcinoma cells. Results Myricetin inhibited the growth of 4T1 and E0771 mouse mammary carcinoma cells to a greater
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Aptamer-linked immobilized sorbent assay for detecting GMO marker, phosphinothricin acetyltransferase (PAT) Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-05-26 Woo-Ri Shin, Mun-Jong Lee, Simranjeet Singh Sekhon, Ji Hun Kim, Sun Chang Kim, Byung-Kwan Cho, Ji-Young Ahn, Yang-Hoon Kim
Background Development of genetically modified crops has rapidly increased in last few years. The most widely grown GM crops express genes that confer herbicide tolerance and insect resistance. Detection system of GM crops is important for safety evaluation before its consumption. Objective The purpose of this research is to detect GM crops, especially PAT, in food-samples. Results The bar gene (PAT
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Wax-printed well pads and colorimetric LAMP detection of ApxIA toxin gene Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-05-25 SeonHyung Lee, Ji Hun Kim, Beom-Ku Han, Won-Il Kim, Byung-Kwan Cho, Sung Min Woo, Yang-Hoon Kim, Ji-Young Ahn
Background Actinobacillus pleuropneumoniae (A. pleuropneumoniae) produces hemolytic cytotoxins, ApxI, ApxII, ApxIII, and ApxIV, causing a huge loss to the swine industry. Objective We developed the PadLysis protocol for extracting genomic DNA (gDNA) for the LAMP—LeucoCrystal Violet (LCV) colorimetric detection. A paper-based well pad for gDNA extraction was fabricated using a wax printing method. Furthermore
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The role of miRNA-155 in monocrotaline-induced pulmonary arterial hypertension through c-Fos/NLRP3/caspase-1 Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-05-25 Shou-Dong Chai, Zhen-Kun Li, Rui Liu, Tao Liu, Ming-Feng Dong, Pei-Zhe Tang, Jian-Tang Wang, Sheng-Jun Ma
Background Nisoldipine can effectively suppress pulmonary arterial smooth muscle cell proliferation and c-Fos expression. Objective To identify the mechanism of the anti-inflammatory effects in monocrotaline-induced pulmonary arterial hypertension (PAH), focusing on the c-Fos/NLRP3/caspase-1 pathway. Results In a mice model of monocrotaline-induced PAH, miRNA-155 expression was increased. In an in
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Polystyrene microplastics induce mortality through acute cell stress and inhibition of cholinergic activity in a brine shrimp Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-05-18 Hye-Jin Eom, Sang-Eun Nam, Jae-Sung Rhee
Backgrounds The environmental impacts of microplastics (MPs) are increasingly of concern, particularly for their sources, global distribution, and persistency in aquatic ecosystems. Although the ubiquitous and persistent nature of MPs in waterbodies is evident, their effects and health implications for aquatic animals are still open to debate. Objective The brine shrimp, Artemia franciscana was exposed
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Pulmonary inflammation caused by silica dioxide nanoparticles in mice via TXNIP/NLRP3 signaling pathway Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-05-11 Je-Oh Lim, Je-Won Ko, Tae-Yang Jung, Woong-Il Kim, So-Won Pak, In-Sik Shin, Won-Kee Yun, Hyoung-Chin Kim, Jeong-Doo Heo, Jong-Choon Kim
Background Silica dioxide nanoparticles (SiONPs) have been used for various medical applications, including therapeutics and imaging, and the use of SiONPs has increased gradually over the years. However, despite an increase in the use of SiONPs, not much is known about mechanism of action of SiONPs and their pulmonary toxicity. Objective The present study investigated the pulmonary toxicity of SiONPs
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Critical role of bile acid (BA) in the cellular entry and permissiveness of Hepatitis B virus in vitro Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-05-08 Hong-Jai Lee, Bo-Young Shin, Jae-Seung Moon, Ailyn Fadriquela, Selikem Abla Nuwormegbe, Chun-Chang Ho, Jin-Su Shin, Jee-Sang Yoon, Sang-Kyou Lee, Soo-Ki Kim
Background Lack of universal replication system for hepatitis B virus with narrow host range and organ tropism has hampered to uncover the pathogenesis of HBV. Previously, we reported the essentiality of humoral milieu and its components toward HBV and hepatitis C virus survival/viability in vitro. Of these components, the precise role of enterohepatic humoral milieu such as bile acid (BA) on HBV cultivation
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Deciphering the possible role of H 2 O 2 in methylmercury-induced neurotoxicity in Xenopus laevis Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-05-08 Xufeng Fu, Xiuyu Yang, Xing Du, Qinghua Cui
Backgrounds Methylmercury (MeHg) is regarded as a developmental neurotoxicant but the detailed mechanism remains not completely clear. Methods The Xenopus laevis embryos were exposed to methylmercury chloride and the expression of neurodevelopment and oxidative stress genes was detected by qRT-PCR or Western blotting. PC12 cells were exposed to various levels of H2O2, and then cell cycle, neurite length
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Characteristic molecular signature of pericardial effusion identifies malignant cancer in pericardial disorder patients Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-04-03 Sang Yean Kim, Jung Woo Eun, Hyung Seok Kim, Hee Doo Yang, Min Jeong Na, Young Min Ahn, Hae Ok Jung, Suk Woo Nam
Background Pericardial effusion (PE) can develop in patients with virtually any condition that affects the pericardium, including acute pericarditis and a variety of systemic disorders. Thus, definite differentiation of malignant pericardial effusion and rapid diagnosis are known to have therapeutic and prognostic importance. Objective The aim of this study was to identify novel molecular markers for
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Screening of cytotoxic and genotoxic potency of two pesticides (malathion and cypermethrin) on Allium cepa L. Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-04-03 Nilofer Sheikh, Himangshu Patowary, Rafiul Amin Laskar
Background Malathion (organophosphate) and cypermethrin (pyrethroid) are widely in use to facilitate protection of major food crops in agriculture, due to which it is important to understand their toxic potential. Allium cepa L. has been considered as a reliable genetic model to detect the toxicity of all sorts of pollutants. Objective The objective of the present work is to determine the cytotoxic
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Trichloroethylene injures rat liver and elevates the level of peroxisomal bifunctional enzyme (Ehhadh) Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-04-02 Nuanyuan Luo, Qunqun Chang, Xiaohu Ren, Peiwu Huang, Wei Liu, Li Zhou, Yungang Liu, Jianjun Liu
Background Trichloroethylene (TCE) is a common industrial solvent and an occupational toxicant. TCE exposure can cause severe hepatotoxicity, but its mode of action is poorly understood. Many studies have shown TCE-induced liver damage in mice, while few have examined the effects of TCE in rats. Objective To explore the effects of TCE in Sprague–Dawley (SD) rats and the potential mechanisms in TCE-induced
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Green tea ameliorates the side effects of the silver nanoparticles treatment of Ehrlich ascites tumor in mice Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-03-31 Ahmed Magdy, Emad Sadaka, Nemany Hanafy, Mohammed A. El-Magd, Nasr Allahloubi, Maged El Kemary
Background Silver nanoparticles (AgNPs) have anti-cancer effects with fewer side effects than standard chemotherapeutic agents, however, they exert oxidative stress-based adverse effects on normal cells and so their applications have raised concern about possible health and environmental risks. Objective We evaluated whether green tea extract (GTE), which contains potent antioxidants, could ameliorate
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Mitochondrial DNA content and deletion ratio are associated with metabolic syndrome in a general population exposed to pesticide Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-03-31 Jung Ran Choi, Sungjin Park, Sung-Kyung Kim, Jae-Yeop Kim, Kyungsuk Lee, Sung-Soo Oh, Sang Baek Koh
Background Mitochondrial DNA (mtDNA) copy number and deletion ratio may be asymmetrically determined using physiologic or pathologic conditions. Mitochondrial dysfunction is associated with an increased risk of developing metabolic syndrome (MetS). Objective Here, we investigated the association between mtDNA copy number and/or deletion ratio and the risk of developing MetS in a general population
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Molecular hydrogen protects against oxidative stress-induced RAW 264.7 macrophage cells through the activation of Nrf2 and inhibition of MAPK signaling pathway Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-03-17 Rahima Begum, Cheol-Su Kim, Ailyn Fadriquela, Johny Bajgai, Xingyu Jing, Dong-Heui Kim, Soo-Ki Kim, Kyu-Jae Lee
Background Oxidative stress is involved in the development of many inflammatory, metabolic and aging diseases. Objective In this study we investigated, the protective effects of H2 on RAW 264.7 macrophage cell against LPS-and H2O2-induced oxidative stress by the inhibition of MAPK pathway and also activate the Nrf2 pathway. Results Our results showed H2 increased the macrophage cell proliferation and
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Glypican 2 regulates cell proliferation and metastasis in thyroid cancer cells Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-03-12 Tao Zhang, Yahong Liu, Xuan Ren, Zhuanping Wang, Hongjuan Wang
Background Glypicans play a role in the growth and metastasis of several human cancers. However, little is known about its function in thyroid cancer. Therefore, this study aimed to investigate the specific functions of glypican 2 (GPC2) in thyroid cancer cells. Objective The expression of six glypican family members (GPC1 to GPC6) was detected in different thyroid cancer cell lines. The abnormal expression
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The role and mechanism of lncRNA NEAT1 in the fibrosis of pulmonary epithelial cell Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-02-19 Hui Xu, YanBo Chen, Jinqiang Zhuang, Shun Zhu, Bing Xu, Jiang Hong
Background Pulmonary fibrosis is a serious clinical fatal disease. Epithelial–mesenchymal transition (EMT) and lncRNA NEAT1 have been implied in its development and progression. Objective To study the role of lncRNA NEAT1 in the progression of fibrosis in human pulmonary epithelial cells (BEAS-2B). Specifically, BEAS-2B was transfected with NEAT1 and miR-29c, EMT and cell proliferation were measured
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Flavonoids in Resina Draconis protect against pulmonary fibrosis via the TGF-β1/NOTCH1 pathway Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-01-22 Liteng Yang, Xin Liu, Ning Zhang, Gaohui Wu, Lifang Chen, Jingyi Xu, Xi Ren, Xiaoming Jiang, Zhijing Huang
Background It is known that flavonoids in Resina Draconis (FRD) have anti-inflammatory and analgesic effects, but the function and mechanisms of FRD against pulmonary fibrosis remain unknown. Objective The study aimed to study the effect and mechanism of FRD on pulmonary fibrosis. Methods Pulmonary fibroblasts were isolated and identified. After treatment with transforming growth factor (TGF)-β1 and
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Anti-inflammatory effect of Rosa laevigata extract on in vitro and in vivo model of allergic asthma via the suppression of IgE and related cytokines Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-01-09 Seung-Hyeon Lee, Seung-Han Choi, In-Seung Lee, Yumi Kim, Eun-Jin An, Hyeung-Jin Jang
Backgrounds Exposure to a toxic stress environment leads to excessive inflammatory reactions and induces allergic asthma resulting in airway hyper-responsiveness. We investigated whether Rosa laevigata Michx. (RL) exhibits anti-inflammatory effects related allergic asthma in both an in vitro and in vivo studies. Methods To investigate the preventive effect of RL, A549 cells were pretreated with non-toxic
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Attenuated anti-tumor activity of NK-92 cells by invasive human breast carcinoma MDA-MB-231 cells Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-01-08 Hwan Hee Lee, Hyosun Cho
Background Natural killer (NK) cells are typical innate lymphocytes that directly kill cancer cells. However, the anti-tumor function of NK cells can be modulated by various cancers in a tumor microenvironment. Objective We investigated whether the functional characteristics of NK cells are affected by coculture with human breast carcinoma MCF-7 or MDA-MB231 cells and further examined the underlying
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3,4-Dichloroaniline promotes fatty liver in zebrafish larvae Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-01-08 Ji-Seon Park, Jeongah Song, Jong-Su Park, Sangwoo Lee, Jieon Lee, Han-Jin Park, Woo-Keun Kim, Seokjoo Yoon, Hang-Suk Chun
Backgrounds 3,4-Dichloroaniline (3,4-DCA) is a transformation product of herbicides that is commonly used as a reference in developmental toxicity studies (OECD TG 236) (Bonnet et al. in Environ Toxicol 22:78–91, 2007). However, the mechanisms underlying 3,4-DCA-induced hepatotoxicity are not well known. Methods We exposed zebrafish larvae at 72 hpf to 3,4-DCA for 3 days and observed lipid accumulation
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Abnormal differentiation of regulatory T cells and Th17 cells induced by perinatal bisphenol A exposure in female offspring mice Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-01-08 You-dan Dong, Liang Gao, Feng-juan Wu, Ren Lin, Yuan Meng, Li-hong Jia, Xiao-fei Wang
Background Bisphenol A (BPA) is an environmental estrogen widely exposed to human beings, and there are more studies on its reproductive toxicity, endocrine disruption and neurobehavioral disorders. Recent few studies have found that BPA has immunotoxicity, and its mechanism is not clear. Therefore, the effects of BPA on immune system have attracted extensive attention. The aim of this study was to
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MiRNA-338-5p reduced inflammation through TXNIP/NLRP3 inflammasome axis by CXCR4 in DSS-induced colitis Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-01-08 Xunru Huang, Yijuan Lin, Xueyan Zheng, Chengdang Wang
Backgrounds miRNAs, about 20–25 bases in length, are short-chain non-coding RNAs existing in the body, which are widely involved in the biological regulation of the organism. miRNAs inhibit the expression of target genes by specifically binding to target genes, thereby exerting biological effects. Methods We investigated the function of miRNA-338-5p on inflammation and its possible mechanisms in colitis
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Particulate matter (PM) 2.5 affects keratinocytes via endoplasmic reticulum (ER) stress-mediated suppression of apoptosis Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-01-08 J. H. Kim, J. W. Son, J. Kim, M. G. Kim, S. H. Jeong, T. J. Park, S. W. Son, H. J. Ryu
Background Particulate matter (PM)2.5 is a concern for public health nowadays. Although few studies have reported the skin diseases associated with PM2.5, its effects on keratinocytes have yet to be elucidated. Objective The goal of this experiment was to analyze and identify the changes of gene expression in PM2.5-treated keratinocytes using RNA-sequencing (RNA-Seq) data. Results PM2.5-treated keratinocytes
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Methylation quantitative trait loci analysis in Korean exposome study Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2020-01-08 Jaehyun Park, Sung Ok Kwon, San-Ha Kim, Seung Jun Kim, Eun Jung Koh, Sungho Won, Woo Jin Kim, Seung Yong Hwang
Background Environmental exposure and genotype variation influence DNA methylation. Studies on the effects of genotype variation were performed mainly on European ancestries. We analyzed the genetic effects on cord blood methylation of Koreans. Methods As part of the Korean Exposome study project, DNA was extracted from 192 cord blood samples for analysis. Cord blood samples were genotyped via Asian
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Toxicoproteomic analysis of deltamethrin exposure in neuroblastoma cell lines Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2019-12-12 Min-Joo Kang, Mi-Young Lee
Background Deltamethrin, a type II pyrethroid insecticide, has been widely used due to its high efficacy against insects and low toxicity to mammals. However, recently, potential adverse health effects during deltamethrin exposure have been reported. Methods A proteomic analysis using two-dimensional electrophoresis with mass spectrometry was performed, and the proteomic data were validated by western
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Adverse effects of subchronic exposure to cooking oil fumes on the gonads and the GPR30-mediated signaling pathway in female rats Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2019-12-11 Xiaoyang Zhang,Yonghua He,Quanhui Lin,Lili Huang,Qunwei Zhang,Youqiong Xu
Background Cooking oil fumes (COFs) are composed of particulate matter, polycyclic aromatic hydrocarbons, volatile organic compounds, aldehydes, and ketones, and are currently a global health concern. Some agents in COFs are mutagenic and carcinogenic. However, only a few reports have addressed the hazardous effects of COF exposure on the female reproductive system. In this study, we explored the effects
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Impact of zinc oxide nanoparticles on the bacterial community of Hydra magnipapillata Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2019-12-09 Ade Yamindago,Nayun Lee,Seonock Woo,Seungshic Yum
Backgrounds Zinc oxide nanoparticles (ZnO NPs) are extensively used for various products. In this study, the effects of ZnO NPs exposure in diversity and community composition of the bacteria associated with H. magnipapillata were investigated. This study provides insight into possible impacts of ZnO NPs on aquatic organisms. Methods 454-pyrosequencing analysis of the bacterial 16S rRNA gene was applied
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Hydra protein reduces the toxicity of Ag–PVP nanoparticles in a 3D A549 cell line Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2019-12-09 Jin Ah Lee, Min-Kyeong Yeo, Soon Seok Kim
Backgrounds Our previous work has confirmed that Hydra magnipapillata extract reduces the toxicity of nanomaterials. We investigated the effects of the Hydra protein (HP) and its role in reducing the toxicity of PVP-coated silver nanoparticles (Ag–PVP NPs) using the A549 lung epithelial cell line. Results The viability of cells exposed to Ag–PVP NPs or Ag–PVP NPs + HP was 77.93 ± 6.96% or 95.04 ± 9
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Propylparaben induces apoptotic cell death in human placental BeWo cells via cell cycle arrest and enhanced caspase-3 activity Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2019-12-06 Mi Jin Kim,Chul-Hong Kim,Mi-Jin An,Ju-Hyun Lee,Geun-Seup Shin,Jae Yoon Hwang,Jinhong Park,Mina Song,Hoyoung Woo,Yeong-hwa Kim,Sangmyung Rhee,Jung-Woong Kim
Backgrounds Propylparaben is one of the most commonly used preservatives in pharmaceuticals, food items, and personal care products. However, growing evidence demonstrates that propylparaben has a harmful effect on various human tissues. In this study, we investigated the cytotoxic effects of propylparaben on the human placental cell line, BeWo. Objective We investigated whether propylparaben caused
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Lipopolysaccharide-dependent transcriptional regulation of PU.1 in microglial cells Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2019-12-05 Chanchal Mandal, Taeho Yoon, Ji Yoon Park, Kyoung Hwa Jung, Young Gyu Chai
Backgrounds PU.1 is a pioneer transcription factor and a master regulator of the myeloid lineage. However, the role of PU.1 in lipopolysaccharide-dependent microglial activation has yet to be investigated. So, this study was conducted to determine the effects of PU.1 in LPS-induced activation of microglial cells. Methods We knocked out PU.1 in murine BV-2 cells using the CRISPR-Cas9 system to investigate
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Multi-tissue lipotoxicity caused by high-fat diet feeding is attenuated by the supplementation of Korean red ginseng in mice Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2019-12-03 Seon-A Jang,Seung Namkoong,Sung Ryul Lee,Jin Woo Lee,Yuna Park,Gyeongseop So,Sung Hyeok Kim,Mi-Ja Kim,Ki-Hyo Jang,Alberto P. Avolio,Sumudu V. S. Gangoda,Hyun Jung Koo,Myung Kyum Kim,Se Chan Kang,Eun-Hwa Sohn
Background Excessive intake of fat, one of the causes of obesity, is associated with low-grade inflammation in various susceptible organs and eventually causes tissue toxicity. This study examines the multifaceted suppressive effects of Korean red ginseng extract (KRG) on high-fat diet (HFD)-induced lipotoxicity and inflammatory responses in the aorta, liver, and brain. Methods Male C57BL/6 mice were
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A toxicogenomics study of two chemicals in coffee roasting process Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2019-12-03 Kyung-Taek Rim,Soo-Jin Kim
Background Roasted coffee beans are broadly consumed in nearly all classes of the population. 2,3-Butanedione is a food flavor that causes obstructive bronchiolitis in microwave popcorn manufacturing workers. It can be naturally produced when coffee is roasted. Objective To determine effects of 2,3-butanedione (diacetyl) and 2,3-pentanedione on workers’ health during the coffee roasting process, we
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Apoptosis and necroptosis-inducing effects of arctigenin on nasal septum carcinoma RPMI-2650 cells in 2D and 3D culture Mol. Cell. Toxicol. (IF 1.66) Pub Date : 2019-12-03 Yoon-Jin Lee, Kwan-Sik Park, Byoung Joon Baek, Kyong-Ae Lee, Sang-Han Lee