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Impacts of high fat diet on ocular outcomes in rodent models of visual disease Exp. Eye Res. (IF 3.011) Pub Date : 2021-01-11 Danielle A. Clarkson-Townsend; Amber J. Douglass; Anayesha Singh; Rachael S. Allen; Ivie N. Uwaifo; Machelle T. Pardue
High fat diets (HFD) have been utilized in rodent models of visual disease for over 50 years to model the effects of lipids, metabolic dysfunction, and diet-induced obesity on vision and ocular health. HFD treatment can recapitulate the pathologies of some of the leading causes of blindness, such as age-related macular degeneration (AMD) and diabetic retinopathy (DR) in rodent models of visual disease
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Neuroprotective effect of the somatostatin receptor 5 agonist L-817,818 on retinal ganglion cells in experimental glaucoma Exp. Eye Res. (IF 3.011) Pub Date : 2021-01-16 Yi Zhang; Na Wu; Qian Li; Xin Hu; Li Wang; Jian-Guo Sun; Zhongfeng Wang; Xing-Huai Sun
Somatostatin plays important roles in modulating neuronal functions by activating the five specific G-protein coupled receptors (sst1-sst5). Previous studies have demonstrated that sst5 were expressed in retinal ganglion cells (RGCs) and sst5 agonist attenuated the α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid-induced retinal neurotoxicity. In this study, we investigated effects and underlying
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Piezo1 plays a role in optic nerve head astrocyte reactivity Exp. Eye Res. (IF 3.011) Pub Date : 2021-01-16 Jiafeng Liu; Yong Yang; Yang Liu
Piezo1 (also known as Fam38A) is a mechanosensing channel required for mechanotransduction in various cell types. In astrocytes, Piezo1 activation is associated with the pathogenesis of central nervous system neurodegeneration. Expression of Piezo1 has been detected in mouse optic nerve head astrocytes, however, the functional role of Piezo1 has not been identified. In this study, we investigated the
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Epigallocatechin-3-gallate increases autophagic activity attenuating TGF-β1-induced transformation of human Tenon's fibroblasts Exp. Eye Res. (IF 3.011) Pub Date : 2021-01-16 Yu Lin Zhang; Yu Qiao Zhang; Hong Liang Lin; Yong Jie Qin; Jin Zeng; Yan Lei Chen; Yong Yi Niu; Chi Pui Pang; Wai Kit Chu; Hong Yang Zhang
We previously found that epigallocatechin-3-gallate (EGCG) could inhibit the myofibroblast transformation of human Tenon's fibroblasts, however, the underlying mechanism remained unclear. We therefore investigated whether the autophagic regulation involved in the anti-fibrotic function of EGCG. The fibroblasts were subjected to transforming growth factor beta-1 (TGF-β1) induction followed by EGCG treatments
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Integrated differential DNA methylation and gene expression of formalin-fixed paraffin-embedded uveal melanoma specimens identifies genes associated with early metastasis and poor prognosis Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-30 Charlotte Ness; Kirankumar Katta; Øystein Garred; Theresa Kumar; Ole Kristoffer Olstad; Goran Petrovski; Morten C. Moe; Agate Noer
Purpose Uveal melanoma (UM) is an aggressive malignancy, in which nearly 50% of the patients die from metastatic disease. Aberrant DNA methylation is recognized as an important epigenomic event in carcinogenesis. Formalin-fixed paraffin-embedded (FFPE) samples represent a valuable source of tumor tissue, and recent technology has enabled the use of these samples in genome-wide DNA methylation analyses
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Intravitreal Interleukin-2 modifies retinal excitatory circuits and retinocollicular innervation Exp. Eye Res. (IF 3.011) Pub Date : 2021-01-15 S.A. Espírito-Santo; N. Nunes-Tavares; H.R. Mendonça; C.A. Serfaty; A. Sholl-Franco; P. Campello-Costa
Interleukin-2 is a classical immune cytokine whose neural functions have received little attention. Its levels have been found to be increased in some neuropathologies, such as Alzheimer's disease, multiple sclerosis and uveitis. Mechanistically, it has been demonstrated the role of IL-2 in regulating glutamate and acetylcholine transmission, thus being relevant for CNS physiology. In fact, our previous
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An in vitro system to investigate IOL: Lens capsule interaction Exp. Eye Res. (IF 3.011) Pub Date : 2021-01-07 Arjun Jaitli; Joyita Roy; Sara Mcmahan; Jun Liao; Liping Tang
Posterior capsule opacification (PCO) is the most common complication associated with intraocular lens (IOL) implantation. Unfortunately, current in vitro models cannot be used to assess the potential of PCO due to their failure to simulate the posterior curvature of the lens capsule (LC) and IOL, a factor known to affect PCO pathogenesis in clinic. To overcome such a challenge, a new system to study
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Hydrogen sulfide is synthesized endogenously in both retinal artery and retina mostly via CSE Exp. Eye Res. (IF 3.011) Pub Date : 2021-01-14 Selçuk Takır; Ayça Toprak Semiz; B.Sönmez Uydeş Doğan
Hydrogen sulfide (H2S) is an important gasotransmitter expressed in various tissues of the organism, including the eye. It is known that H2S is localized especially in the retina and corneal layers in bovine eye. The enzymes that mediate H2S synthesis are 3-mercaptopyruvate sulfurtransferase (3-MST), cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE). Herein, we aimed to investigate the
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Ionising radiation causes vision impairment in neonatal B6C3F1 mice Exp. Eye Res. (IF 3.011) Pub Date : 2021-01-14 Daniel Pawliczek; Helmut Fuchs; Valerie Gailus-Durner; Martin Hrabě de Angelis; Jochen Graw; Claudia Dalke
Ionising radiation interacts with lenses and retinae differently. In human lenses, posterior subcapsular cataracts are the predominant observation, whereas retinae of adults are comparably resistant to even relatively high doses. In this study, we demonstrate the effects of 2 Gy of low linear energy transfer ionising radiation on eyes of B6C3F1 mice aged postnatal day 2. Optical coherence tomography
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Anterior scleral thickness and shape changes with different levels of simulated convergence Exp. Eye Res. (IF 3.011) Pub Date : 2021-01-06 Hamed Niyazmand; Scott A. Read; David A. Atchison; David Alonso-Caneiro; Michael J. Collins
Purpose Convergence plays a fundamental role in the performance of near visual tasks. We measured the effect of two levels of convergence on anterior scleral thickness and shape in emmetropes, low to moderate myopes and high myopes. Methods Forty-five healthy young adults aged between 18 and 35 years including 15 emmetropes, 15 low/moderate myopes, and 15 high myopes were recruited. Anterior segment
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Topically instilled caffeine selectively alters emmetropizing responses in infant rhesus monkeys Exp. Eye Res. (IF 3.011) Pub Date : 2021-01-09 Earl L. Smith; Li-Fang Hung; Zhihui She; Krista Beach; Lisa A. Ostrin; Monica Jong
Oral administration of the adenosine receptor (ADOR) antagonist, 7-methylxanthine (7-MX), reduces both form-deprivation and lens-induced myopia in mammalian animal models. We investigated whether topically instilled caffeine, another non-selective ADOR antagonist, retards vision-induced axial elongation in monkeys. Beginning at 24 days of age, a 1.4% caffeine solution was instilled in both eyes of
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Establishment of a pigmented murine model abundant with characteristics of retinal vein occlusion Exp. Eye Res. (IF 3.011) Pub Date : 2021-01-13 Sugao Miyagi; Anri Nishinaka; Takumi Yamamoto; Wataru Otsu; Shinsuke Nakamura; Masamitsu Shimazawa; Takashi Kitaoka; Hideaki Hara
Retinal vein occlusion (RVO) is a vascular disease that represents characteristic retinal hemorrhage and dilated retinal veins. Despite its clinical importance, its pathogenesis remains largely unknown because of limited opportunities to acquire human retinal samples. Therefore, an animal model that reproduces the clinical features of RVO patients is required for further investigation. In this study
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Efficacy of topotecan nanoparticles for intravitreal chemotherapy of retinoblastoma Exp. Eye Res. (IF 3.011) Pub Date : 2021-01-13 Elham Delrish; Mahmoud Jabbarvand; Fariba Ghassemi; Fahimeh Asadi Amoli; Fatemeh Atyabi; Alireza Lashay; Masoud Soleimani; Leila Aghajanpour; Rassoul Dinarvand
Retinoblastoma (Rb) is the most common intraocular malignancy in children that accounts for approximately 4% of all pediatric malignancies. Since chemotherapy is a widely practiced treatment for Rb, there is a growing interest in developing new and effective drugs to overcome systemic and local side effects of chemotherapy to improve the quality of life and increase the chances of survival. This study
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Intravitreal melphalan hydrochloride vs propylene glycol-free melphalan for retinoblastoma vitreous seeds: Efficacy, toxicity and stability in rabbits models and patients Exp. Eye Res. (IF 3.011) Pub Date : 2021-01-11 Carley M. Bogan; Janene M. Pierce; Stephanie Doss; Yuankai K. Tao; Sheau-chiann Chen; Kelli L. Boyd; Albert Liao; Terry Hsieh; David H. Abramson; Jasmine H. Francis; Debra L. Friedman; Ann Richmond; Anthony B. Daniels
The use of intravitreal chemotherapy has revolutionized the treatment of advanced intraocular retinoblastoma, as intravitreal melphalan has enabled difficult-to-treat vitreous tumor seeds to be controlled, leading to many more eyes being saved. However, melphalan hydrochloride (MH) degrades rapidly in solution, increasing logistical complexity with respect to time between medication preparation and
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Knockdown of TRIM5α or TRIM11 increases lentiviral vector transduction efficiency of human muller cells Exp. Eye Res. (IF 3.011) Pub Date : 2021-01-10 Monica M. Sauter; Curtis R. Brandt
The goal of this study was to determine the expression and distribution of the host restriction factors (RFs) TRIM5α and TRIM11 in non-human primate (NHP) neural retina tissue and the human Muller cell line MIO-M1. In addition, experiments were performed to determine the effect of TRIM5α and TRIM11 knockdown on FIVGFP transduction of MIO-M1 cells with the goal of devising strategies to increase the
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The protective function of invariant natural killer T cells in the relapse of experimental autoimmune uveoretinitis Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-19 Natsumi Tajiri; Taiki Kato; Masashi Satoh; Misao Iizuka; Masaru Taniguchi; Nobuyoshi Kitaichi; Kazuya Iwabuchi
Experimental autoimmune uveoretinitis (EAU) in mice provides a useful platform to study the pathogenesis and experimental therapeutics of human uveitis. One often used EAU model employs C57BL/6 (B6) mice sensitized with a peptide residue having 1 to 20 amino acids of human interphotoreceptor retinoid binding protein (hIRBP1-20). The model using the B6 background has permitted a liberal use of genetically
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The effect of nerve growth factor on corneal nerve regeneration and dry eye after LASIK Exp. Eye Res. (IF 3.011) Pub Date : 2021-01-02 Qianwen Gong; Shasha Zhang; Lu Jiang; Meng Lin; Zhiqiang Xu; Ye Yu; Qinmei Wang; Fan Lu; Liang Hu
Dry eye is the most common complication after refractive surgery, especially after laser in situ keratomileusis (LASIK), in which nerves may be cut when making the corneal flap. Nerve growth factor (NGF) has been demonstrated to stimulate corneal sensitivity and nerve regeneration and NGF has been suggested as a potential treatment for dry eye disease (DED). Hence, this study aimed to investigate the
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Minocycline prevents the inflammatory response after retinal detachment, where microglia phenotypes being regulated through A20 Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-14 Wenna Gao; Jiantong Du; Ying Chi; Ruilin Zhu; Xinran Gao; Liu Yang
Retinal detachment (RD) is a severe sight-threatening complication that can be caused by a multitude of retinal diseases. It has been evidenced that minocycline exerts neuroprotective effects by targeting microglia in the pathogenesis of massive ocular lesions including RD, but mechanisms remain elusive. We carried out this research to elucidate the potential mediators that link RD-induced vision loss
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Effect of aflibercept on proliferative vitreoretinopathy: Proteomic analysis in an experimental animal model Exp. Eye Res. (IF 3.011) Pub Date : 2021-01-05 Isil Uslubas; Aylin Kanli; Murat Kasap; Gurler Akpinar; Levent Karabas
Purpose The aim of this study was to monitor inflammatory, proliferative and progressive effects of proliferative vitreoretinopathy (PVR) and aflibercept treatment in dispase induced PVR rat model by proteomic analysis. Material and methods A total of 35 male Long Evans pigmented rats were divided into three groups, namely, PVR (dispase+saline), PVR+aflibercept (dispase+aflibercept) and control. The
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Using bioinformatic protein sequence similarity to investigate if SARS CoV-2 infection could cause an ocular autoimmune inflammatory reactions? Exp. Eye Res. (IF 3.011) Pub Date : 2021-01-02 Işıl Kutlutürk Karagöz; Marion R. Munk; Mücahit Kaya; René Rückert; Mustafa Yıldırım; Levent Karabaş
Although severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) infection have emerged globally, findings related to ocular involvement and reported cases are quite limited. Immune reactions against viral infections are closely related to viral and host proteins sequence similarity. Molecular Mimicry has been described for many different viruses; sequence similarities of viral and human tissue
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circRNA-miRNA-mRNA network in age-related macular degeneration: From construction to identification Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-29 Yu Su; Yuexiong Yi; Lu Li; Changzheng Chen
The aim of the present study was to investigate the pathogenesis of age-related macular degeneration (AMD) by constructing a regulatory circRNA-miRNA-mRNA network. By adjusting the P value to <0.05 and the absolute log value of fold change to >0.25, 2920 and 1057 differentially expressed mRNAs were identified from GSE50195 and GSE29801, respectively. Based on a literature review, Starbase database
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Descriptive analysis of Fibulin-3 and the extracellular vesicle marker, Alix, in drusen from a small cohort of postmortem human eyes Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-30 Stephanie L. Grillo; Justin D. Etzel; Sarah R. Weber; Cassandra Ondeck; Weiwei Wang; Yuanjun Zhao; Alistair J. Barber; Jeffrey M. Sundstrom
Fibulin-3 (Fib3) is a secreted glycoprotein that is expressed in the retina and has been associated with drusen formation in age-related macular degeneration (AMD). The purpose of this study was to assess whether Fib3 is associated with extracellular vesicles (EVs) in drusen from non-diseased and AMD human donors. De-identified sections of human eyes were received from the National Disease Research
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Automated segmentation and analysis of retinal microglia within ImageJ Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-24 Neil F. Ash; Michael T. Massengill; Lindsey Harmer; Ahmed Jafri; Alfred S. Lewin
Microglia are immune cells of the central nervous system capable of distinct phenotypic changes and migration in response to injury. These changes most notably include the retraction of fine dendritic structures and adoption of a globular, phagocytic morphology. Due to their characteristic responses, microglia frequently act as histological indicators of injury progression. While algorithms seeking
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Age-related increase of let-7 family microRNA in rat retina and vitreous Exp. Eye Res. (IF 3.011) Pub Date : 2021-01-04 Priscilla Sayami Akamine; Cilene Rebouças Lima; Gabriela Jesus Lustoza Costa; Cesar Seigi Fuziwara; Carolina Beltrame Del Debbio; Edna Teruko Kimura; Marinilce Fagundes Santos; Dânia Emi Hamassaki
Vitreous alterations occur from early stages and continue through the normal aging, with gradual lamellae formation and the appearance of liquefied spaces, which eventually leads to complications, such as retinal tear, retinal detachment, and intravitreal hemorrhage. The aim of the present study was to investigate the expression of let-7 miRNA family in the vitreous and retina in newborn (1-3- day-old)
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Visualization of different anatomical parts of the enucleated human eye using X-ray micro-CT imaging Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-10 Sergey Y. Tkachev; Boris I. Mitrin; Nikolay S. Karnaukhov; Evgeniy V. Sadyrin; Mark V. Voloshin; Alexey Y. Maksimov; Anna S. Goncharova; Ekaterina A. Lukbanova; Ekaterina V. Zaikina; Anastasiya V. Volkova; Darya V. Khodakova; Maria V. Mindar; Marina A. Yengibarian; Tatyana P. Protasova; Sergey O. Kit; Alexey M. Ermakov; Sergey V. Chapek; Marina S. Tkacheva
Abstract. Micro-CT visualization allows reconstruction of eye structures with the resolution of light microscopy and estimation of tissue densities. Moreover, this method excludes damaging procedures and allows further histological staining due to the similar steps in the beginning. We have shown the feasibility of the lab-based micro-CT machine usage for visualization of clinically important compartments
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In-vivo imaging for assessing tumor growth in mouse models of ocular melanoma Exp. Eye Res. (IF 3.011) Pub Date : 2021-01-03 Ortal Zaks; Dimitri Gaber; Keren Ben-Yaakov; Maya Sharvit-Bader; Alexa Goz; Ziv Rotfogel; Shahar Ish-Shalom; Evgenia Filipova; Ady Yosipovich; Hana Leiba; Martine J. Jager; Arie L. Marcovich; Michal Aharoni-Simon
Uveal melanoma (UM) and conjunctival melanoma (CM) are ocular malignancies that give rise to life-threatening metastases. Although local disease can often be treated successfully, it is often associated with significant vision impairment and treatments are often not effective against metastatic disease. Novel treatment modalities that preserve vision may enable elimination of small tumors and may prevent
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Roots of Lithospermum erythrorhizon promotes retinal cell survival in optic nerve crush-induced retinal degeneration Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-28 Tae Kyeom Kang; Tam Thi Le; Kyung-A Kim; Young-Joo Kim; Wook-Bin Lee; Sang Hoon Jung
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Effect of inhalation anesthesia with isoflurane on circadian rhythm of murine intraocular pressure Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-24 Shunsuke Tsuchiya; Tomomi Higashide; Sachiyo Hatake; Kazuhisa Sugiyama
Purpose For research on circadian intraocular pressure (IOP), rebound tonometers are widely used with or without general anesthesia as a non-invasive approach to obtain IOP values. However, whether general anesthesia such as inhalation anesthesia with isoflurane affects the circadian rhythm of IOP and in turn IOP measurements is currently unclear. As such, data reporting IOP values obtained under general
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Transcriptomics analysis of Ccl2/Cx3cr1/Crb1rd8 deficient mice provides new insights into the pathophysiology of progressive retinal degeneration Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-26 Anna Badia; Anna Salas; Anna Duarri; Bárbara Ferreira-de-Souza; Miguel Ángel Zapata; Laura Fontrodona; José García-Arumí
Chronic oxidative stress and immune dysregulation are key mechanisms involved in the pathogenesis of most retinal degenerative diseases, including age-related macular degeneration. The Ccl2−/−/Cx3cr1−/−/Crb1rd8/rd8 mouse model develops a progressive degeneration phenotype, with photoreceptor atrophy, drusen-like lesions or pigment alterations at an early age; however, the role of oxidative stress and
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Multimodal imaging and electroretinography highlights the role of VEGF in the laser-induced subretinal fibrosis of monkey Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-29 Yujiao Wang; Qiyao Fang; Chaomao Zhang; Yongjiang Chen; Tao Gou; Qinglin Cai; Hongyu Yin; Yunxia Gao; Yuliang Feng; Shuang Qiu; Ming Zhang; Xiaobo Cen; Hui Zhang; Danian Chen
Age-related macular degeneration (AMD) is a leading cause of blindness. Laser-induced nonhuman primate choroidal neovascularization (CNV) is a widely used animal model of neovascular AMD. Subretinal fibrosis (SFb) is the major limiting factor of effective anti-VEGF therapy for neovascular AMD, yet SFb has never been systematically analyzed in the primate CNV model and if VEGF directly affect SFb is
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Alterations in the conjunctival surface bacterial microbiome in bacterial keratitis patients Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-23 Sisinthy Shivaji; Rajagopalaboopathi Jayasudha; Sama Kalyana Chakravarthy; Chinthala Reddy SaiAbhilash; Gumpili Sai Prashanthi; Savitri Sharma; Prashant Garg; Somasheila I. Murthy
Microbial keratitis is an infectious disease of the eye, in which the cornea is inflamed. Under severe conditions, keratitis can lead to significant loss of vision and enucleation of the eye. Ocular trauma is the major risk factor causing keratitis and microorganisms viz., bacteria, fungi, viruses are the causative agents. The current study characterized the conjunctival bacterial microbiomes of healthy
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A new hand-held holder optimizes the parameters of the laser-induced choroidal neovascularization model in mice Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-15 Bai Xue; Shanshan Zhang; Li Gan; Weifeng Lu; Jie Li
Background The laser-induced choroidal neovascularization (CNV) mouse model, as the most classic animal model of age-related macular degeneration (AMD), has been widely used. We designed a hand-held mouse holder to optimize mouse fixation in the laser-induced CNV modelling process, which was inconvenient until now. This study aimed to evaluate the effectiveness of our in-house hand-held mouse holder
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Effects of mammalian target of rapamycin inhibitors on fibrosis after trabeculectomy Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-24 Nozomi Igarashi; Megumi Honjo; Makoto Aihara
Glaucoma, the second leading cause of blindness worldwide, is characterized by aberrant elevations of intraocular pressure (IOP), which can damage the optic nerve. IOP reduction is the only effective therapy for prevention of visual impairment and blindness in both hypertensive and normotensive individuals, and in some cases, trabeculectomy is a major surgical procedure that can lower IOP in patients
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Effect of isolation method on human corneal stromal cell behaviour Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-25 Thomas L.A. Volatier; Francisco C. Figueiredo; Che J. Connon
Current research on healthy corneal stromal cells will typically use primary cells as they are the most representative of in vivo behaviour. Primary cells are normally isolated from the limbus of discarded donor peripheral corneal tissue left over from transplantation (due to its relative abundance). Therefore, the central part of the cornea is less used in research as this tissue is usually used for
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Involvement of the NLRC4 inflammasome in promoting retinal ganglion cell death in an acute glaucoma mouse model Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-14 Ke Yao; Yin Zhao; Peiming Jin; Xiaotong Lou; Zhaoxia Luo; Hong Zhang; Fei Li
Purpose To explore the role of nucleotide-binding oligomerization domain-like receptors (NLRs) family caspase-activation and the recruitment domain containing 4 (NLRC4) inflammasome in retinal ganglion cell (RGC) injury induced by an acute glaucoma mouse model. Method A mouse model of acute ocular hypertension, which can lead to retinal ischemia-reperfusion (I/R) injury, was established. The expression
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Evolution of oxidative stress, inflammation and neovascularization in the choroid and retina in a subretinal lipid induced age-related macular degeneration model Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-08 Soo-Young Kim; Siva P. Kambhampati; Imran A. Bhutto; D. Scott McLeod; Gerard A. Lutty; Rangaramanujam M. Kannan
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Spectrum-frequency and genotype–phenotype analysis of rhodopsin variants Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-18 Hualei Luo; Xueshan Xiao; Shiqiang Li; Wenmin Sun; Zhen Yi; Panfeng Wang; Qingjiong Zhang
Mutations in RHO are the most common cause of autosomal dominant retinitis pigmentosa. However, the pathogenicity of many RHO variants is questionable. This study was designed to investigate the genotype–phenotype correlation for RHO variants. These RHO variants were collected from the in-house exome sequencing data of 7092 probands suffering from different types of eye conditions. The variants were
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Rapamycin ameliorates corneal injury after alkali burn through methylation modification in mouse TSC1 and mTOR genes Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-23 Jiande Li; Shaobo Du; Yongpeng Shi; Jiangyuan Han; Zhanyu Niu; Li Wei; Pengfei Yang; Linchi Chen; Huanbing Tian; Lan Gao
Alkali burn to the cornea is one of the most intractable injuries to the eye due to the opacity resulting from neovascularization (NV) and fibrosis. Numerous studies have focused on studying the effect of drugs on alkali-induced corneal injury in mouse, but fewer on the involvement of alkali-induced DNA methylation and the PI3K/AKT/mTOR signaling pathway in the mechanism of alkali-induced corneal injury
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Plasma metabolomic profiling of central serous chorioretinopathy Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-14 Hui Xu; Lvzhen Huang; Enzhong Jin; Zhiqiao Liang; Mingwei Zhao
Our study aimed to investigate metabolites alterations in the blood plasma of central serous chorioretinopathy (CSC) patients and to identify the key biomarkers to increase the understanding of the mechanism of CSC at the molecular level. Quantitative and targeted metabolomics using liquid chromatography tandem-mass spectrometry (LCMS, Biocrates P500) assays were performed on plasma samples from the
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Progress in developing rodent models of age-related macular degeneration (AMD) Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-17 Sudha Priya Soundara Pandi; J. Arjuna Ratnayaka; Andrew J. Lotery; Jessica L. Teeling
Age-related macular degeneration (AMD) is the leading cause of irreversible central vision loss, typically affecting individuals from mid-life onwards. Its multifactorial aetiology and the lack of any effective treatments has spurred the development of animal models as research and drug discovery tools. Several rodent models have been developed which recapitulate key features of AMD and provide insights
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Development and optimization of a personalized fibrin membrane derived from the plasma rich in growth factors technology Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-14 Eduardo Anitua; María de la Fuente; Francisco Muruzabal; Jesús Merayo-Lloves
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Iatrogenic corneal diseases or conditions Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-17 José Álvaro Pereira Gomes; José Arthur Pinto Milhomens Filho
Any prescribed or self-administered therapy carries inherent risks of secondary adverse events. While the volume of treatments being administered through healthcare systems has been increasing, scientific advancements in our understanding of the mechanisms of pharmaceutical side effects and complications from procedures now allow us to reduce the risk of non-intentional damage to ocular health. This
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A comparison of the ultrastructure of the cornea of the pre- and post-metamorphic axolotl (Ambystoma mexicanum, Amphibia) Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-09 Shaun P. Collin; H. Barry Collin
The corneal ultrastructure of the pre- and post-metamorphic stages of the neotenic axolotl Ambystoma mexicanum is examined using light microscopy and both scanning and transmission electron microscopy to reveal whether there are any morphological changes associated with a switch in lifestyle. Although the complement of corneal layers remains the same, there are significant quantitative changes in corneal
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Update on the genetics of keratoconus Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-13 Yelena Bykhovskaya; Yaron S. Rabinowitz
In the past few years we have seen a great acceleration of discoveries in the field of keratoconus including new treatments, diagnostic tools, genomic and molecular determinants of disease risk. Recent genome-wide association studies (GWAS) of keratoconus cases and population wide studies of variation in central corneal thickness and in corneal biomechanical properties confirmed already identified
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USH2A-retinopathy: From genetics to therapeutics Exp. Eye Res. (IF 3.011) Pub Date : 2020-10-27 Lyes Toualbi; Maria Toms; Mariya Moosajee
Bilallelic variants in the USH2A gene can cause Usher syndrome type 2 and non-syndromic retinitis pigmentosa. In both disorders, the retinal phenotype involves progressive rod photoreceptor loss resulting in nyctalopia and a constricted visual field, followed by subsequent cone degeneration, leading to the loss of central vision and severe visual impairment. The USH2A gene raises many challenges for
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Quantification of ocular surface microcirculation by computer assisted video microscopy and diffuse reflectance spectroscopy Exp. Eye Res. (IF 3.011) Pub Date : 2020-10-22 Anne Kari Kvernebo; Takuma Miyamoto; Ane Hasle Sporastøyl; Liv Kristin Wikslund; Svein-Erik Måsøy; Liv Drolsum; Morten Carstens Moe; Goran Salerud; Kiyotaka Fukamachi; Knut Kvernebo
In piglets we tested the applicability of digital video microscopy and diffuse reflectance spectroscopy for non-invasive assessments of limbal and bulbar conjunctival microcirculation. A priori we postulated that the metabolic rate is higher in limbal as compared to bulbar conjunctiva, and that this difference is reflected in microvascular structure or function between the two locations. Two study
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Association of plasma trace element levels with neovascular age-related macular degeneration Exp. Eye Res. (IF 3.011) Pub Date : 2020-10-21 Thomas J. Heesterbeek; Mansour Rouhi-Parkouhi; Stephanie J. Church; Yara T. Lechanteur; Laura Lorés-Motta; Nikolaos Kouvatsos; Simon J. Clark; Paul N. Bishop; Carel B. Hoyng; Anneke I. den Hollander; Richard D. Unwin; Anthony J. Day
Although the triggers causing angiogenesis in the context of neovascular age-related macular degeneration (nAMD) are not fully understood, oxidative stress is likely involved. Oxidative stress in the eye can occur through exposure of macular tissues to sunlight and local or systemic exposure to oxidative stressors associated with environmental or lifestyle factors. Because trace elements have been
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The human Descemet's membrane and lens capsule: Protein composition and biomechanical properties Exp. Eye Res. (IF 3.011) Pub Date : 2020-11-02 Willi Halfter; Suzette Moes; Kathrin Halfter; Monica S. Schoenenberger; Christophe A. Monnier; Joanna Kalita; Daphne Asgeirsson; Tatjana Binggeli; Paul Jenoe; Hendrik P.N. Scholl; Paul Bernhard Henrich
The Descemet's membrane (DM) and the lens capsule (LC) are two ocular basement membranes (BMs) that are essential in maintaining stability and structure of the cornea and lens. In this study, we investigated the proteomes and biomechanical properties of these two materials to uncover common and unique properties. We also screened for possible protein changes during diabetes. LC-MS/MS was used to determine
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Differential Lysyl oxidase like 1 expression in pseudoexfoliation glaucoma is orchestrated via DNA methylation Exp. Eye Res. (IF 3.011) Pub Date : 2020-11-12 Alison G. Greene; Sarah B. Eivers; Fiona McDonnell; Edward W.J. Dervan; Colm J. O'Brien; Deborah M. Wallace
Pseudoexfoliation syndrome (PXF) is the most common cause of secondary open angle glaucoma worldwide. Single nucleotide polymorphisms (SNPs) in the gene Lysyl oxidase like 1 (LOXL1) are strongly associated with the development of pseudoexfoliation glaucoma (PXFG). However, these SNPs are also present in 50–80% of the general population, suggestive of other factors being involved in the pathogenesis
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Optical transmittance and ultrastructure of SMILE-derived lenticules subjected to three different preservative methods Exp. Eye Res. (IF 3.011) Pub Date : 2020-11-13 Fei Xia; Jing Zhao; Dan Fu; Ye Xu; Peijun Yao; Meiyan Li; Aruma Aruma; Xingtao Zhou
Purpose To investigate the optical transmittance and ultrastructure of small incision lenticule extraction (SMILE)-derived lenticules preserved via three different methods. Methods A total of 90 lenticules obtained from myopic patients undergoing SMILE surgery were divided into control and experimental groups. Fresh lenticules served as the control. The preserved lenticules of the experimental group
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Inflammatory basis for dry eye disease flares Exp. Eye Res. (IF 3.011) Pub Date : 2020-10-08 Victor L. Perez; Michael E. Stern; Stephen C. Pflugfelder
Most patients with chronic dry eye disease (DED) have episodic flares, which can be triggered by a variety of activities and environmental stresses. These flares are typically associated with rapid exacerbation of discomfort symptoms, followed by prolonged elevation of inflammation. In an acute flare, ocular surface inflammation begins with a nonspecific innate immune response, in some cases followed
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H2O2-induced cataract as a model of age-related cataract: Lessons learned from overexpressing the proteasome activator PA28αβ in mouse eye lens Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-09 Malin Hernebring; Julia Adelöf; John Wiseman; Anne Petersen; Madeleine Zetterberg
Cataract, the world-leading cause of blindness, is formed when crystallin aggregates cloud the eye lens. We overexpressed PA28αβ, a proteasome activator with properties protective against aggregation and oxidative stress, and examined whether they are less prone to develop cataract arisen from aging and/or hydrogen peroxide (H2O2) treatment. Another objective of this work was to compare the H2O2-induced
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Optimal timing for activation of sigma 1 receptor in the Pde6brd10/J (rd10) mouse model of retinitis pigmentosa Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-09 Jing Wang; Haiyan Xiao; Shannon Barwick; Yutao Liu; S.B. Smith
Sigma 1 Receptor (Sig1R), a pluripotent modulator of cell survival, is a promising target for treatment of retinal degenerative diseases. Previously, we reported that administration of the high-affinity, high-specificity Sig1R ligand (+)-pentazocine, ((+)-PTZ) beginning at post-natal day 14 (P14) and continuing every other day improves visual acuity and delays loss of photoreceptor cells (PRCs) in
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A novel organic cation transporter involved in paeonol transport across the inner blood-retinal barrier and changes in uptake in high glucose conditions Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-08 Asmita Gyawali; Myung-Hee Kim; Young-Sook Kang
Paeonol exerts various pharmacological effects owing to its antiangiogenic, antioxidant, and antidiabetic activities. We aimed to investigate the transport mechanism of paeonol across the inner blood-retinal barrier both in vitro and in vivo. The carotid artery single injection method was used to investigate the retina uptake index of paeonol. The retina uptake index (RUI) value of [³H]paeonol was
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Clinical and genetic analysis of the ABCA4 gene associated retinal dystrophy in a large Chinese cohort Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-07 Zixi Sun; Lizhu Yang; Hui Li; Xuan Zou; Lei Wang; Shijing Wu; Tian Zhu; Xing Wei; Yong Zhong; Ruifang Sui
ABCA4 gene associated retinal dystrophies (ABCA4-RD) are a group of inherited eye diseases caused by ABCA4 gene mutations, including Stargardt disease, cone-rod dystrophy and retinitis pigmentosa. With the development of next-generation sequencing (NGS), numerous clinical and genetic studies on ABCA4-RD have been performed, and the genotype and phenotype spectra have been elucidated. However, most
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Different modes of full-thickness macular hole formation Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-07 Andreas Bringmann; Jan Darius Unterlauft; Thomas Barth; Renate Wiedemann; Matus Rehak; Peter Wiedemann
Full-thickness macular holes (FTMH) are an important cause of visual deterioration. However, different modes of FTMH formation are less investigated. It is also not clear whether the development of edematous cysts contributes to FTMH formation. In this retrospective case series of 30 eyes of 30 patients, we describe using spectral-domain optical coherence tomography different modes of FTMH formation
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Connexin43 hemichannel block inhibits NLRP3 inflammasome activation in a human retinal explant model of diabetic retinopathy Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-05 Henry H. Louie; Avik Shome; Charisse YJ. Kuo; Ilva D. Rupenthal; Colin R. Green; Odunayo O. Mugisho
Diabetic retinopathy (DR), the most common ocular complication associated with diabetes, is a chronic vascular and inflammatory disease that leads to vision loss. The inflammasome pathway, a key part of the innate immune system, is required to activate chronic inflammation in DR. Unfortunately, current therapies for DR target pathological signs that are downstream of the inflammasome pathway, making
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GSDMD, an executor of pyroptosis, is involved in IL-1β secretion in Aspergillus fumigatus keratitis Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-03 Wenyi Zhao; Hua Yang; Leyu Lyu; Jie Zhang; Qiang Xu; Nan Jiang; Guibo Liu; Limei Wang; Haijing Yan; Chengye Che
The protein GSDMD is an important performer of pyroptosis and a universal substrate for the inflammatory caspase. However, the role and regulatory mechanism of GSDMD in Aspergillus fumigatus keratitis is remains unknown. Here we detected GSDMD protein in the cornea of normal and fungal-infected C57BL/6 mice. Human corneal epithelial cell (HCECs) were preincubated with a hydrochloride solution (IFNR
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Ectatic diseases Exp. Eye Res. (IF 3.011) Pub Date : 2020-12-01 Marcella Q. Salomão; Ana Luisa Hofling- Lima; Louise Pellegrino Gomes Esporcatte; Fernando Faria Correa; Bernardo Lopes; Nelson Sena; Daniel G. Dawson; Renato Ambrósio
Ectatic corneal disease (ECD) comprises a group of disorders characterized by progressive thinning and subsequent bulging of the corneal structure. Different phenotypes have been recognized, including keratoglobus, pellucid marginal degeneration (PMD), and keratoconus (KC). Keratoconus has been widely investigated throughout the years, but the advent of laser refractive surgery boosted an immediate
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An efficient inducible RPE-Selective cre transgenic mouse line Exp. Eye Res. (IF 3.011) Pub Date : 2020-11-29 Ming Chen; Lily Kim; Carolyn W. Lu; Hong Zeng; Douglas Vollrath