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Drp1-dependent mitochondrial fragmentation mediates photoreceptor abnormalities in type 1 diabetic retina Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-11 Shuyu Tang, Mengling Huang, Ruixuan Wang, Ming Li, Ning Dong, Ronghan Wu, Zailong Chi, Ling Gao
Recent studies have highlighted that retinal neurodegeneration precedes microvascular changes in diabetic retinopathy (DR), but the specific mechanisms remain unclear. Given the pivotal role of dysfunctional mitochondria and oxidative stress in early DR, our objective was to observe mitochondria-related alterations in the neural retina of type one diabetic mellitus mice with no evidence of DR (T1DM-NDR)
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Optical development in the murine eye lens of accelerated senescence-prone SAMP8 and senescence-resistant SAMR1 strains Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-11 Kehao Wang, Yutian Pu, Leran Chen, Masato Hoshino, Kentaro Uesugi, Naoto Yagi, Xiaoyong Chen, Yuu Usui, Akira Hanashima, Ken Hashimoto, Satoshi Mohri, Barbara K. Pierscionek
The eye lens is responsible for focusing objects at various distances onto the retina and its refractive power is determined by its surface curvature as well as its internal gradient refractive index (GRIN). The lens continues to grow with age resulting in changes to the shape and to the GRIN profile. The present study aims to investigate how the ageing process may influence lens optical development
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Rho-kinase inhibitor alleviates CD4+T cell mediated corneal graft rejection by modulating its STAT3 and STAT5 activation Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-11 Shang Li, Peng Zhang, Ao Li, Jiayu Bao, Zhiqiang Pan, Ying Jie
Penetrating keratoplasty remains the most common treatment to restore vision for corneal diseases. Immune rejection after corneal transplantation is one of the major causes of graft failure. In recent years, Rho-associated protein kinase (ROCK) inhibitors have been found to be associated with the activation of the STATs pathway and are widely studied in autoimmune diseases. Therefore, it may be possible
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Modeling autosomal dominant retinitis pigmentosa by using patient-specific retinal organoids with a class-3 RHO mutation Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-11 Xiao Lin, Zhuo-Lin Liu, Xiao Zhang, Wen Wang, Zhi-Qin Huang, Shu-Ning Sun, Zi-Bing Jin
Rhodopsin-mediated autosomal dominant retinitis pigmentosa (RHO-adRP) causes progressive vision loss and is potentially incurable, accounting for 25% of adRP cases. Studies on RHO-adRP mechanism were at large based on the biochemical and cellular properties, especially class-3. Nonetheless, the absence of an appropriate model for class-3 RHO-adRP has impeded comprehensive exploration. Here, induced
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Stop codon variant in EFEMP1 is associated with primary open-angle glaucoma due to impaired regulation of aqueous humor outflow Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-11 Junkai Tan, Suping Cai, Xiaolin Luo, Qiang Li, Yanbing Chen, Zijie Chen, Yukai Mao, Guo Liu, Mingming Yang, Xuyang Liu
It is known that the actin cytoskeleton and its associated cellular interactions in the trabecular meshwork (TM) and juxtacanalicular tissues mainly contribute to the formation of resistance to aqueous outflow of the eye. Fibulin-3, encoded by gene, has a role in extracellular matrix (ECM) modulation, and interacts with enzymatic ECM regulators, but the effects of fibulin-3 on TM cells has not been
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Sphingolipid biosynthetic inhibitor L-cycloserine prevents oxidative-stress-mediated death in an in vitro model of photoreceptor-derived 661W cells Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-08 Faiza Tahia, Sandip K. Basu, Amanda Prislovsky, Koushik Mondal, Dejian Ma, Harry Kochat, Kennard Brown, Daniel J. Stephenson, Charles E. Chalfant, Nawajes Mandal
Oxidative stress plays a pivotal role in the pathogenesis of several neurodegenerative diseases. Retinal degeneration causes irreversible death of photoreceptor cells, ultimately leading to vision loss. Under oxidative stress, the synthesis of bioactive sphingolipid ceramide increases, triggering apoptosis in photoreceptor cells and leading to their death. This study investigates the effect of L-Cycloserine
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Intraocular pressure across the lifespan of Tg-MYOCY437H mice Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-05 Xiaoyan Zhang, Gaiping Xi, Pengchao Feng, Cong Li, Markus H. Kuehn, Wei Zhu
Transgenic C57BL/6 mice expressing human myocilin (Tg-MYOC) are a well-established model for primary open-angle glaucoma (POAG). While the reduced trabecular meshwork (TM) cellularity due to severe endoplasmic reticulum (ER) stress has been characterized as the etiology of this model, there is a limited understanding of how glaucomatous phenotypes evolve over the lifespan of Tg-Myoc mice. In this study
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Analysis of the mucosal chemokines CCL28, CXCL14, and CXCL17 in dry eye disease: An in vitro and clinical investigation Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-05 Alfredo Domínguez-López, Marta Blanco-Vázquez, Andrés Ángel Calderón-García, Carmen García-Vázquez, María J. González-García, Margarita Calonge, Amalia Enríquez-de-Salamanca
Mucosal chemokines have antimicrobial properties and play an important role in mucosal immunity. However, little is known about their expression on the ocular surface. This study aimed to analyze the expression of the mucosal chemokines CCL28, CXCL14 and CXCL17 in corneal and conjunctival epithelial cells under dry eye (DE) conditions, and in conjunctival samples from healthy subjects and DE patients
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Targeting mechanics-induced trabecular meshwork dysfunction through YAP-TGFβ Ameliorates high myopia-induced ocular hypertension Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-05 Qianwen Bu, Hai Zhu, Guangliang Cao, Ganyu Gong, Ying Su, Qingshu Ge, Wei Zhu, Zongyi Li, Xiaojing Pan
High myopia is a risk factor for primary open angle glaucoma (POAG). The pathological mechanism of high myopia induced POAG occurrence is not fully understood. In this study, we successfully established the guinea pig model of ocular hypertension with high myopia, and demonstrated the susceptibility of high myopia for the occurrence of microbead-induced glaucoma compared with non-myopia group and the
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Oleic acid induces lipogenesis and NLRP3 inflammasome activation in organotypic mouse meibomian gland and human meibomian gland epithelial cells Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-05 Ren Liu, Mengqian Tu, Jianwen Xue, Bing Xiao, Jing Li, Lingyi Liang
The accumulation of oleic acid (OA) in the meibum from patients with meibomian gland dysfunction (MGD) suggests that it may contribute to meibomian gland (MG) functional disorder, as it is a potent stimulator of acne-related lipogenesis and inflammation in sebaceous gland. Therefore, we investigate whether OA induces lipogenesis and inflammasome activation in organotypic cultured mouse MG and human
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Characterization of lncRNA and mRNA profiles in ciliary body in experimental myopia Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-29 Shanshan Wu, Jiawen Hao, Dadong Guo, Zhongyu Ma, Qiuxin Wu, Ming Zhang, Hongsheng Bi
Currently, researchers have mainly focused on the role of the tissues of the posterior segment of the eyes in the development of myopia. However, the ciliary body, an anterior ocular tissue that contracts to initiate the process of accommodation, may also play an important role in the progression of myopia due to the increased demand for near work. In the present study, we established a lens-induced
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Causal associations of refractive error and early age-related macular degeneration: A Mendelian randomization study Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-27 Lingling Wang, Wenlong Wei, YongJi Zhao, Sixi Chen, Dongjing Wu, Mengjun Tu
This study aims to determine the risk associated with early age-related macular degeneration (AMD) due to refractive errors (RE) using an analysis of genome-wide association study (GWAS) data through the two-sample Mendelian randomization approach. Single-nucleotide polymorphisms (SNPs) linked to refractive errors (RE) were obtained from numerous GWAS studies involving individuals of European descent
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Detection of retinal dysfunction induced by HCN channel inhibitors using multistep light stimulus and long-duration light stimulus ERG in rats Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-23 Naohisa Umeya, Yuki Yoshizawa, Kosuke Fukuda, Keigo Ikeda, Mami Kamada, Hiroshi Inada, Toru Usui, Izuru Miyawaki
Ivabradine, a hyperpolarization-activated cyclic nucleotide–gated (HCN) channel inhibitor, has been reported to induce photosensitivity-related visual disturbances such as phosphene in humans. Ivabradine-induced visual disturbances are caused by inhibition of HCN channels in the retina, and the mechanisms have been verified using HCN channel knockout mice and electroretinography (ERG). However, in
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Extracellular vesicle biomarkers in ocular fluids associated with ophthalmic diseases Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-22 Thomas Michael Shiju, Alex Yuan
Extracellular vesicles (EVs) are released as highly stable lipid bilayer particles carrying proteins, lipids, glycans and miRNAs. The contents of EVs vary based on the cellular origin, biogenesis route and the functional state of the cell suggesting certain diseased conditions. A growing body of evidence show that EVs carry important molecules implicated in the development and progression of ophthalmic
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Isolation and characterization of rabbit limbal niche cells Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-22 Guanyu Su, Xiaojie Guo, Lingjuan Xu, Bihui Jin, Yongyao Tan, Xiao Zhou, Wei Wang, Xinyu Li, Shusheng Wang, Guigang Li
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TGF-β2-induced alteration of m6A methylation in hTERT RPE-1 cells Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-21 Xue Li, Xueru Zhao, Ruijie Yin, Min Yuan, Yongya Zhang, Xiaohua Li
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Therapeutic effect and mechanism of action of pterostilbene nano drugs in dry eye models Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-20 Kexin Li, Meng Lin, Kaiyan Huang, Jiaxin Han, Linzhi Wei, Lijie Miao, Huijuan Chen, Qianwen Gong, Xingyi Li, Liang Hu
Dry eye disease is a multifactorial dysfunction of the tear film and ocular surface, with etiology involving inflammation and oxidative stress on the ocular surface. Pterostilbene (PS) is a secondary metabolite extracted from plants, which possesses remarkable anti-inflammatory and antioxidant effects. However, its application is limited by light instability and very poor water solubility. We modified
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Human lens epithelial-secreted exosomes attenuate ocular angiogenesis via inhibiting microglial activation Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-20 Yan Wu, Jiagui Wang, Ting Pan, Jie Lei, Yuanyuan Fan, Jingfan Wang, Changlin Xu, Qinyuan Gu, Xingxing Wang, Tianhao Xiao, Qinghuai Liu, Ping Xie, Zizhong Hu
The lens is an avascular tissue, where epithelial cells (LECs) are the primary living cells. The role of LECs-derived exosomes (LEC-exos) is largely unknown. In our study, we determined the anti-angiogenic role of LEC-exos, manifested as regressed retinal neovascularization (NV) using the oxygen-induced retinopathy (OIR), and reduced choroidal NV size and pathological vascular leakage using the laser-induced
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The response of retinal ganglion cells to optical defocused visual stimuli in mouse retinas Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-19 Chunghim So, Ting Zhang, Qin Wang, Chunting Qiu, De Lestrange-Anginieur Elie, Feng Pan
Myopia and astigmatism are two primary types of refractive errors characterized by inaccurate focusing images on the retina. This study aimed to investigate the response characteristics of Retinal Ganglion Cells (RGCs), represented by alpha (α) RGCs, when exposed to focused, simulated defocused pattern images and astigmatically defocused images projected onto mouse retinas.
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Long noncoding RNA ZFAS1: A novel anti-apoptotic target in Fuchs endothelial corneal dystrophy Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-17 Jini Qiu, Ruiping Gu, Qian Shi, Xueling Zhang, Jiayu Gu, Jun Xiang, Jianjiang Xu, Yujing Yang, Kun Shan
Fuchs endothelial corneal dystrophy (FECD) is the leading cause of endothelial keratoplasty without efficacious drug treatment. Recent studies have emphasized the involvement of epigenetic regulation in FECD development. Long non-coding RNAs (lncRNAs) are recognized as crucial epigenetic regulators in diverse cellular processes and ocular diseases. In this study, we revealed the expression patterns
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Influence of the calcium voltage-gated channel auxiliary subunit (CACNA2D1) absence on intraocular pressure in mice Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-17 Levi Lankford, Rupalatha Maddala, Monica M. Jablonski, P. Vasantha Rao
The etiology of elevated intraocular pressure (IOP), a major risk factor for glaucoma (optic nerve atrophy), is poorly understood despite continued efforts. Although the gene variant of (encoding α2δ1), a calcium voltage-gated channel auxiliary subunit, has been reported to be associated with primary open-angle glaucoma, and the pharmacological mitigation of α2δ1 activity by pregabalin lowers IOP,
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Allele-specific antisense oligonucleotides for the treatment of BEST1-Related dominantly inherited retinal diseases: An in vitro model Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-16 Beren Karaosmanoglu, Gozde Imren, Eda Utine, Hande Taylan Sekeroglu, Ekim Z. Taskiran
Retinal dystrophies are a common health problem worldwide that are currently incurable due to the inability of retinal cells to regenerate. Inherited retinal diseases (IRDs) are a diverse group of disorders characterized by progressive vision loss caused by photoreceptor cell dysfunction. The eye has always been an attractive organ for the development of novel therapies due to its independent access
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Oxymatrine mitigates Aspergillus fumigatus keratitis by suppressing fungal activity and restricting pyroptosis Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-15 Weichen Liu, Xue Tian, Lingwen Gu, Bing Yu, Ziyi Wang, Menghui Chi, Jing Lin, Qian Wang, Guibo Liu, Guiqiu Zhao
Fungal keratitis (FK) is a refractory keratitis caused by excessive inflammation and fungal damage. Excessive inflammation can lead to tissue damage and corneal opacity, resulting in a poor prognosis for FK. Oxymatrine (OMT) is a natural alkaloid, which has rich pharmacological effects, such as antioxidant and anti-inflammation. However, its antifungal activity and the mechanism of action in FK have
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Gut microbiota compositional profile in patients with posner-schlossman syndrome Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-13 Ning Wang, Chengyang Sun, Yahan Ju, Lulu Huang, Yixin Liu, Mengyang Gu, Chenrui Xu, Minghan Wang, Yue Wu, Dandan Zhang, Li Xu, Wenyi Guo
The cause of Posner-Schlossman syndrome (PSS) remains unknown and its frequent recurrence may eventually lead to irreversible damage of the optic nerve. The influence of immune factors in the pathophysiology of PSS is gaining more and more interest. Increasing evidence suggests that gut dysbiosis plays vital roles in a variety of neurodegenerative and immune-related diseases. However, alterations of
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Verification of the gene and protein expression of the aquaglyceroporin AQP3 in the mammalian lens Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-12 Rosica S. Petrova, Nishanth Francis, Kevin L. Schey, Paul J. Donaldson
Transport of water is critical for maintaining the transparency of the avascular lens, and the lens is known to express at least five distinctly different water channels from the Aquaporin (AQP) family of proteins. In this study we report on the identification of a sixth lens AQP, AQP3 an aquaglyceroporin, which in addition to water also transports glycerol and HO. AQP3 was identified at the transcript
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CircRNA expression profiles and regulatory networks in the vitreous humor of people with high myopia Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-12 Liyue Zhang, Xin Yu, Nan Hong, Yutong Xia, Xuhong Zhang, Liyin Wang, Chen Xie, Feng Dong, Jianping Tong, Ye Shen
Myopia is a global health and economic issue. Circular RNAs (circRNAs) have been shown to play an important role in the pathogenesis of many ocular diseases. We first evaluated the circRNA profiles and possible roles in vitreous humor samples of individuals with high myopia by a competitive endogenous RNA (ceRNA) array. Vitreous humor samples were collected from 15 high myopic (5 for ceRNA array, and
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MiR-181a-5p may regulate cell proliferation and autophagy in myopia and the associated retinopathy Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-12 Bo Jiang, Nan Hong, Liyue Zhang, Baisheng Xu, Qin He, Xilin Qian, Feidi Li, Feng Dong
The mechanism of myopia and the associated retinopathy remains unclear, and dysregulated microRNAs (miRNAs) are implicated in this disease. In this research, we purposed to find out the regulatory function that miRNAs play in myopia and the associated retinopathy. We first performed miRNA microarray analysis in a lens-induced myopia mouse model and found that miR-9-5p, miR-96–5p, miR-182–5p, miR-183–5p
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Revisiting rabbit models for keratoconus: A long-term study on collagenase-induced disease progression Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-11 Sujithra Shankar, Minal Thacker, Abhishek Sahoo, Aniruth Reddy Aindla, Khyathi Ratna Padala, Jilu Jaffet, Kiran Kumar Bokara, Sayan Basu, Vivek Singh
Keratoconus (KC) is one of the degenerative corneal disorders consequential of degradation of network of collagen fibrils in cornea leading to thinning and cone shaped cornea. Various studies have established animal models of KC by inducing collagenase II to gain a better understanding of the pathogenesis, however, long-term monitoring/follow-up of the models were not reported so far. This study evaluates
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GSDMD mediated pyroptosis induced inflammation of Graves’ orbitopathy via the NF-κB/ AIM2/ Caspase-1 pathway Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-09 Xiaotong Sha, Huijing Ye, Xing Wang, Zhihui Xu, Anqi Sun, Wei Xiao, Te Zhang, Shenglan Yang, Huasheng Yang
Gasdermin D (GSDMD) is a key executor which triggers pyroptosis as well as an attractive checkpoint in various inflammatory and autoimmune diseases but it has yet to prove its function in Graves'orbitopathy (GO). Our aim was to investigate GSDMD levels in orbital connective tissue and serum of GO patients and then assess the association between serum levels and patients' clinical activity score (CAS)
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Comprehensive analysis of the miRNA-mRNA regulatory network involved in spontaneous recovery of an H2O2-induced zebrafish cataract model Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-09 Jiawei Luo, Mu Zhang, Yanhua Chen, Guowei Zhang, Tianqiu Zhou, Lihua Kang, Xiaoqing Chen, Huaijin Guan
To identify the hub miRNAs and mRNAs contributing to the spontaneous recovery of an HO-induced zebrafish cataract model. Zebrafishes were divided into three groups, i.e., Group A, which included normal control fish (day 0), and Groups B and C, where fish were injected with 2.5% hydrogen peroxide into the anterior chamber and reared for 14 and 30 days, respectively. Fish eyes were examined by stereomicroscope
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CircSTRBP contributes to H2O2-induced lens epithelium cell dysfunction through increasing NOX4 mRNA stability by recruiting IGF2BP1 Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-09 Di Li, Xuanyi Che, Ningning Gao, Jing Li
Previous studies have shown that the development of age-related cataract (ARC) is involved in lens epithelium dysfunction, which is associated with abnormally expressed circular RNAs (circRNAs). The current work aims to probe the role of circSTRBP (hsa_circ_0088,427) in hydrogen peroxide (HO)-induced lens epitheliums. Lens epithelium tissues were harvested from ARC or normal subjects (n = 23). CircSTRBP
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Compensation of inner retina to early-stage photoreceptor degeneration in a Rho mouse model of retinitis pigmentosa Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-08 Bingjie Wang, Riley K. Arbuckle, Katherine A. Davoli, Owen D. Clinger, Richard Brown, José-Alain Sahel, Yuanyuan Chen, Shaohua Pi
Retinitis pigmentosa (RP) is an inherited retinal disorder characterized by the degeneration of photoreceptors. mice, which carry a Pro23His mutation in the gene, are one of the most studied animal models for RP. However, except for the photoreceptors, other retinal neural cells have not been fully investigated in this model. Here, we record the temporal changes of the retina by optical coherence tomography
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Heterogenous thinning of peripapillary tissues occurs early during high myopia development in juvenile tree shrews Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-07 Mahmoud T. KhalafAllah, Preston A. Fuchs, Fred Nugen, Mustapha El Hamdaoui, Alexander M. Levy, Brian C. Samuels, Rafael Grytz
Myopia is an independent risk factor for glaucoma, but the link between both conditions remains unknown. Both conditions induce connective tissue remodeling at the optic nerve head (ONH), including the peripapillary tissues. The purpose of this study was to investigate the thickness changes of the peripapillary tissues during experimental high myopia development in juvenile tree shrews. Six juvenile
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Isorhamnetin suppresses the epithelial-mesenchymal transition of the retinal pigment epithelium both in vivo and in vitro through Nrf2-dependent AKT/GSK-3β pathway Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-07 Qinyi Hui, Ning Yang, Caijian Xiong, Siqi Zhou, Xin Zhou, Qingzi Jin, Xinrong Xu
Age-related macular degeneration (AMD) is a major cause of blindness in the elderly worldwide. Multiple studies have shown that epithelial-mesenchymal transition (EMT) plays a pivotal role in the pathogenesis of AMD. Isorhamnetin (Isor) is a flavonoid compound that inhibits EMT in tumor cells. However, whether it can also attenuate EMT in the retinal pigment epithelium (RPE) is unknown. Therefore,
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Lysyl oxidase like-1 deficiency in optic nerve head astrocytes elicits reactive astrocytosis and alters functional effects of astrocyte derived exosomes Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-06 Harsh N. Hariani, Anita K. Ghosh, Sasha M. Rosen, Huen-Yee Tso, Cassidy Kessinger, Chongyu Zhang, W. Keith Jones, Rebecca M. Sappington, Claire H. Mitchell, Evan B. Stubbs, Vidhya R. Rao, Simon Kaja
Glaucoma is a multifactorial progressive ocular pathology that manifests clinically with damage to the optic nerve (ON) and the retina, ultimately leading to blindness. The optic nerve head (ONH) shows the earliest signs of glaucoma pathology, and therefore, is an attractive target for drug discovery. The goal of this study was to elucidate the effects of reactive astrocytosis on the elastin metabolism
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Impact of culture media on primary human corneal endothelial cells derived from old donors Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-03 Alessia Merra, Eleonora Maurizi, Graziella Pellegrini
Corneal endothelial dysfunction is a major indication for corneal transplantation. However, a global shortage of donor corneal tissues and risks associated with corneal surgeries have prompted exploration of alternative options, including tissue-engineered grafts or cell injection therapy. Nonetheless, these approaches require a controlled culture of primary human corneal endothelial cells (HCEnCs)
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Establishment of mouse model of neurotrophic keratopathy through TRPV1 neuronal ablation Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-02 Leilei Zhao, Rong Chen, Jingyu Qu, Lingling Yang, Ya Li, Linyan Ma, Xinyi Zang, Xia Qi, Xiaolei Wang, Qingjun Zhou
Neurotrophic keratopathy (NK) is a challenging disease with the reduced innervation to the cornea. To establish a genetic and stable mouse model of NK, we utilized the TRPV1-DTR mice with intraperitoneal injection of diphtheria toxin (DT) to selectively eliminate TRPV1 neurons. After DT administration, the mice exhibited robust ablation of TRPV1 neurons in the trigeminal ganglion, accompanied with
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Transcriptome alterations in sf3b4-depleted zebrafish: Insights into cataract formation in retinitis pigmentosa model Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-02 Zulvikar Syambani Ulhaq, Yukiko Ogino, William Ka Fai Tse
Posterior subcapsular cataract (PSC) frequently develops as a complication in patients with retinitis pigmentosa (RP). Despite numerous scientific investigations, the intricate pathomechanisms underlying cataract formation in individuals affected by RP remain elusive. Therefore, our study aims to elucidate the potential pathogenesis of cataracts in an RP model using splicing factor subunit 3b () mutant
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A method for generating zonular tension in the murine eye by embedding and compressing the globe in a hydrogel Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-02 Wade Rich, Manqi Pan, Jun Liu, Katelyn E. Swindle-Reilly, Matthew A. Reilly
The ocular lens is the primary organ within the eye responsible for accommodation. During accommodation, the lens is subject to biomechanical forces. We previously demonstrated that stretching the porcine lens can increase lens epithelial cell proliferation. Although murine lenses are commonly employed in lens research, murine lens stretching has remained unexplored. Murine lens stretching thus represents
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Investigating the therapeutic potential of Allium cepa extract in combating pesticide exposure induced ocular damage Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-02 Shalini Sanyal, Praveen K. Vemula, Sujata Law
The ocular surface is subject to a range of potentially hazardous environmental factors and substances, owing to its anatomical location, sensitivity, and physiological makeup. Xenobiotic stress exerted by chronic pesticide exposure on the cornea is primarily responsible for ocular irritation, excessive tear production (hyper-lacrimation), corneal abrasions and decreased visual acuity. Traditional
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In vivo quasi-elastic light scattering detects molecular changes in the lenses of adolescents with down syndrome Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-02 Srikant Sarangi, Olga Minaeva, Danielle M. Ledoux, Douglas S. Parsons, Juliet A. Moncaster, Caitlin A. Black, Jeffrey Hollander, Yorghos Tripodis, John I. Clark, David G. Hunter, Lee E. Goldstein
Down syndrome (DS) is the most common chromosomal disorder in humans. DS is associated with increased prevalence of several ocular sequelae, including characteristic blue-dot cerulean cataract. DS is accompanied by age-dependent accumulation of Alzheimer's disease (AD) amyloid-β (Aβ) peptides and amyloid pathology in the brain and comorbid early-onset Aβ amyloidopathy and colocalizing cataracts in
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In Memoriam: M. Cristina Kenney, MD, PhD (1949–2023) Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-01 Baruch D. Kuppermann, Krzysztof Palczewski
Abstract not available
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Comprehensive exploration of hub genes involved in oxidative stress in rhegmatogenous retinal detachment based on bioinformatics analysis Exp. Eye Res. (IF 3.4) Pub Date : 2024-01-29 Qin Xu, Linbi Luo, Xiaohong Xiang, Yalin Feng, Yang Cao, Jun Zeng, Hongbin Lv
Rhegmatogenous retinal detachment (RRD) is a type of ophthalmologic emergency, if left untreated, the blindness rate approaches 100%. The RRD patient postoperative recovery of visual function is unsatisfactory, most notably due to photoreceptor death. We conducted to identify the key genes for oxidative stress (OS) in RRD through bioinformatics analysis and clinical validation, thus providing new ideas
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High Vasohibin-2 expression correlated with autophagy in proliferative diabetic retinopathy Exp. Eye Res. (IF 3.4) Pub Date : 2024-01-24 Yuzhi Ding, Na Su, Jie Luan, Jian Xu, Shanhu Qiu, Zilin Sun
Vasohibin-2 (VASH2) is confirmed to be associated with angiogenesis. To investigate the vitreous levels of VASH2 and how VASH2 induces angiogenesis in proliferative diabetic retinopathy (PDR), a total of 120 eyes were enrolled in this prospective and randomized controlled study and the vitreous level of VASH2 was quantified by Luminex liquid suspension chip. Vector systems were applied in human retinal
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Deciphering the molecular symphony: Unraveling endothelial-to-mesenchymal transition in corneal endothelial cells Exp. Eye Res. (IF 3.4) Pub Date : 2024-01-20 Zhaoxiang Lu, Haimiao Lin, Jinming Li, Yun Feng
Understanding the molecular complexity of this phenomenon provides innovative targets for maintaining phenotypic integrity during in vitro expansion, thereby advancing corneal endothelial tissue engineering. In this study, we established an in vitro model to simulate endothelial-to-mesenchymal transition (EndMT) in corneal endothelial cells. Through RNA sequencing, we identified 452 upregulated and
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Evaluation of biophysical alterations in the epithelial and endothelial layer of patients with Bullous Keratopathy Exp. Eye Res. (IF 3.4) Pub Date : 2024-01-20 Erick Rafael Dias Rates, Charles Duarte Almeida, Elaine de Paula Fiod Costa, Roberta Jansen de Mello Farias, Ralph Santos-Oliveira, Luciana M. Rebelo Alencar
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Shotgun lipidomics of human subretinal fluids under rod-dominant retina reveals cone-dominated lipids Exp. Eye Res. (IF 3.4) Pub Date : 2024-01-24 Jianzhong Chen, Christine A. Curcio, Jason N. Crosson
Subretinal fluid (SRF) accumulates between photoreceptor outer segments and retinal pigment epithelium during rhegmatogenous retinal detachment. Biomolecular components such as lipids originate from cells surrounding the SRF. Knowledge of the composition of these molecules in SRF potentially provides mechanistic insight into the physiologic transfer of lipids between retinal tissue compartments. Using
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Genistein stimulates the viability and prevents myofibroblastic transformation in human trabecular meshwork cells stimulated by TGF-β Exp. Eye Res. (IF 3.4) Pub Date : 2024-01-23 Robert Wasilewicz, Julia Wasilewicz, Ewa Pruszyńska-Oszmałek, Kinga Stuper – Szablewska, Natalia Leciejewska, Paweł Antoni Kołodziejski
Primary open-angle glaucoma (POAG) is the most common type of glaucoma leading to blindness. The search for ways to prevent/treat this entity is one of the main challenges of today's ophthalmology. One of such solution seems to be biologically active substances of natural origin, such as genistein (GEN), which can affect the function of isolated trabecular meshwork by the inhibition of protein tyrosine
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Identification of dysregulation of sphingolipids in retinoblastoma using liquid chromatography-mass spectrometry Exp. Eye Res. (IF 3.4) Pub Date : 2024-01-19 Omkar Surendra Khade, Sruthy Sasidharan, Ankit Jain, Bhavani Shankar Maradani, Amit Chatterjee, Divya Gopal, Ranjith Kumar Ravi Kumar, Subramaniyan Krishnakumar, Akhilesh Pandey, Narayanan Janakiraman, Sailaja V. Elchuri, Seetaramanjaneyulu Gundimeda
Retinoblastoma (RB) is a rare ocular cancer seen in children that counts for approximately 3% of all childhood cancers. It is found that mutation in RB1, a tumour Suppressor Gene on chromosome 13 as the cause of malignancy. Retinoblastoma protein is the target for ceramide to cause apoptosis. We studied lipidomics of two RB cell lines, one aggressive cell line (NCC–RbC-51) derived from a metastatic
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Metabolomic profiling of ocular tissues in rabbit myopia: Uncovering differential metabolites and pathways Exp. Eye Res. (IF 3.4) Pub Date : 2024-01-18 Chengpeng Liang, Fayuan Li, Chengqi Gu, Ling Xie, Wen Yan, Xiaoye Wang, Rong Shi, Shaorong Linghu, Taixiang Liu
To investigate the metabolic difference among tissue layers of the rabbits’ eye during the development of myopia using metabolomic techniques and explore any metabolic links or cascades within the ocular wall. Ultra Performance Liquid Chromatography - Mass Spectrometry (UPLC-MS) was utilized for untargeted metabolite screening (UMS) to identify the significant differential metabolites produced between
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Permanent transduction of retinal ganglion cells by rAAV2-retro Exp. Eye Res. (IF 3.4) Pub Date : 2024-01-19 Yicen J. Zheng, Mikayla D. Dilbeck, John R. Economides, Jonathan C. Horton
Adeno-associated virus (AAV) is widely used as a vector for delivery of gene therapy. Long term therapeutic benefit depends on perpetual expression of the wild-type gene after transduction of host cells by AAV. To address this issue in a mass population of identified single cells, 4 rats received an injection of a 1:1 mixture of rAAV2-retro-hSyn-EGFP and rAAV2-retro-hSyn-mCherry into each superior
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Expression of Nephronectin in the Descemet's membrane of mouse corneas during development and adult homeostasis Exp. Eye Res. (IF 3.4) Pub Date : 2024-01-19 Matthew Garis, Matthew D. Meyer, Peter Lwigale
Nephronectin (Npnt) is an extracellular matrix (ECM) protein with pleiotropic functions during organogenesis, disease, and homeostasis. Although the ECM plays a crucial role during development and homeostasis of the adult cornea, little is known about the expression of Npnt in the mammalian cornea. Here, we investigated the expression of Npnt during early embryonic and postnatal development, and in
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Corneal stromal localization of TGF beta isoforms in spontaneous persistent epithelial defects after PRK in rabbits Exp. Eye Res. (IF 3.4) Pub Date : 2024-01-17 Valeria Villabona-Martinez, Barbara A.L. Dutra, Lycia P. Sampaio, Marcony R. Santhiago, Steven E. Wilson
The purpose of this study was to evaluate transforming growth factor beta (TGFβ) isoform localization in rabbit corneas with spontaneous persistent epithelial defects (PEDs) after photorefractive keratectomy (PRK). Four cryofixed corneas from a previously reported series of PEDs in rabbits that had PRK were evaluated with triplex immunohistochemistry (IHC) for TGFβ3, myofibroblast marker alpha-smooth
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Scaling up polarized RPE cell supernatant production on parylene membrane Exp. Eye Res. (IF 3.4) Pub Date : 2024-01-17 Dimitrios Pollalis, Alejandra Gonzalez Calle, Juan Carlos Martinez-Camarillo, Kabir Ahluwalia, Cassidy Hinman, Debbie Mitra, Jane Lebkowski, Sun Young Lee, Biju B. Thomas, Faizah Ahmed, Victoria Chan, Jason A. Junge, Scott Fraser, Stan Louie, Mark Humayun
Age-related macular degeneration (AMD), a leading cause of vision loss, primarily arises from the degeneration of retinal pigment epithelium (RPE) and photoreceptors. Current therapeutic options for dry AMD are limited. Encouragingly, cultured RPE cells on parylene-based biomimetic Bruch's membrane demonstrate characteristics akin to the native RPE layer. In this study, we cultivated human embryonic
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Automated hyperspectral imaging for non-invasive characterization of human eye vasculature: A potential tool for ocular vascular evaluation Exp. Eye Res. (IF 3.4) Pub Date : 2024-01-14 Yasser H. El-Sharkawy
The vascular supply to the human eye plays a vital role in maintaining ocular health, making its non-invasive evaluation essential for diagnosing and managing various ocular disorders. This paper presents a novel approach utilizing hyperspectral imaging (HSI) to non-invasively characterize human eye vasculature. The proposed system aims to specifically identify the blood atrium and veins of the human
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Effects of hypoxia in the diabetic corneal stroma microenvironment Exp. Eye Res. (IF 3.4) Pub Date : 2024-01-14 Purnima Sharma, Jian-Xing Ma, Dimitrios Karamichos
Corneal dysfunctions associated with Diabetes Mellitus (DM), termed diabetic keratopathy (DK), can cause impaired vision and/or blindness. Hypoxia affects both Type 1 (T1DM) and Type 2 (T2DM) surprisingly, the role of hypoxia in DK is unexplored. The aim of this study was to examine the impact of hypoxia in vitro on primary human corneal stromal cells derived from Healthy (HCFs), and diabetic (T1DMs
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TRPV: An emerging target in glaucoma and optic nerve damage Exp. Eye Res. (IF 3.4) Pub Date : 2024-01-08 Xiaotong Zhang, Feng Wang, Ying Su
Transient receptor potential vanilloid (TRPV) channels are members of the TRP channel superfamily, which are ion channels that sense mechanical and osmotic stimuli and participate in Ca2+ signalling across the cell membrane. TRPV channels play important roles in maintaining the normal functions of an organism, and defects or abnormalities in TRPV channel function cause a range of diseases, including
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Early regional changes in retina and choroid of chicks following monocular hemifield form deprivation Exp. Eye Res. (IF 3.4) Pub Date : 2024-01-09 Xiaoliao Peng, Yangyi Huang, Yuliang Wang, Jianmin Shang, Yang Shen, Zhi Chen, Xingtao Zhou, Tian Han
To investigate regional changes in the chick retina and choroid after hemifield form deprivation (HFD). Ten chicks were randomly and equally divided into a temporal retinal deprivation (TRD) and nasal retinal deprivation (NRD) group. HFD was induced with half-lateral translucent plastic goggles in the right eye; the left eye was kept untreated. Swept-source optical coherence tomography (SS-OCT) images
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Combined treatment of human mesenchymal stem cells and green tea extract on retinal ganglion cell regeneration in rats after optic nerve injury Exp. Eye Res. (IF 3.4) Pub Date : 2024-01-10 Qichen Yang, Yanxuan Xu, Xin Bin, Kwok Ping Chan, Shaowan Chen, Zhen Qian, Yao Yao, Xiang-Ling Yuan, Kunliang Qiu, Yuqiang Huang, Tsz Kin Ng
Retinal ganglion cell (RGC) death and axonal loss cause irreversible vision loss upon optic nerve (ON) injury. We have independently demonstrated that mesenchymal stem cells (MSCs) and green tea extract (GTE) promote RGC survival and axonal regeneration in rats with ON injury. Here we aimed to evaluate the combined treatment effect of human bone marrow-derived MSCs (hBM-MSCs) and GTE on RGC survival