样式: 排序: IF: - GO 导出 标记为已读
-
Cranial morphological variation of Ctenomys lami (Rodentia: Ctenomyidae) in a restricted geographical distribution. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-11-13 Rodrigo Fornel,Renan Maestri,Pedro Cordeiro-Estrela,Daniela Sanfelice,Thales Renato O de Freitas
The relationship between chromosomal and morphological variation in mammals is poorly understood. We analyzed the cranial size and shape variation in Ctenomys lami concerning to the geographic variation in their chromosome numbers. This subterranean rodent occurs in a narrow range of sand-dunes in the Coastal Plain of southern Brazil. This species presents a high karyotypic variation with diploid numbers
-
Chromosomal characterization of three species of Serrasalmini (Serrasalmidae: Characiformes). Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-11-10 Alan Gomes Dos Santos,José Francisco de Sousa E Souza,Simone Cardoso Soares,Celeste Mutuko Nakayama,Eliana Feldberg
The tribe Serrasalmini is a diverse group with paraphyletic genera and taxonomic uncertainties. Several studies have been carried out in this group of fish in order to understand this problem, including the cytogenetic approach. In this study, three species of a clade of Serrasalmini were characterized cytogenetically - Pristobrycon striolatus, Catoprion absconditus and Pygopristis denticulatus. The
-
Insights into the evolutionary history of the most skilled tool-handling platyrrhini monkey: Sapajus libidinosus from the Serra da Capivara National Park. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-11-10 Thaynara Lima,Bibiana Fam,Gustavo Medina Tavares,Tiago Falótico,Camila Cantele,Lucca Fanti,Luane Landau,Lucas Henriques Viscardi,Pedro Vargas-Pinilla,Ossman Barrientos-Diaz,Alcides Pissinatti,Vinicius A Sortica,Eduardo B Ottoni,Ana Lúcia A Segatto,Andreia Carina Turchetto-Zolet,Maria Cátira Bortolini
Sapajus libidinosus members of the Pedra Furada group, living in the Serra da Capivara National Park, use stone tools in a wider variety of behaviors than any other living animal, except humans. To rescue the evolutionary history of the Caatinga S. libidinosus and identify factors that may have contributed to the emergence and maintenance of their tool-use culture, we conducted fieldwork seasons to
-
Phenotypic and molecular basis for genetic variation in jelly palms (Butia sp.): where are we now and where are we headed to? Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-11-10 Camila Fritzen Cidón,Andreia Carina Turchetto-Zolet,Miklos Maximiliano Bajay,Maria Imaculada Zucchi,Enéas Ricardo Konzen
We compiled studies that addressed morphological and physicochemical traits, as well as population genetic studies involving jelly palms, genus Butia (Arecaceae). First, we conducted a bibliometric study with selected articles, by revising the fundamental contributions to unraveling phenotypic traits that have been used for describing the phenotypic variation within and among populations. Moreover
-
Evolution and diversification of the O-methyltransferase (OMT) gene family in Solanaceae. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-11-10 Pedro Henrique Pezzi,Leonardo Tresoldi Gonçalves,Maríndia Deprá,Loreta Brandão de Freitas
O-methyltransferases (OMTs) are a group of enzymes involved in several fundamental biological processes in plants, including lignin biosynthesis, pigmentation, and aroma production. Despite the intensive investigation of the role of OMTs in plant secondary metabolism, the evolution and diversification of this gene family in Solanaceae remain poorly understood. Here, we conducted a genome-wide survey
-
Genome-based taxonomy of Burkholderia sensu lato: Distinguishing closely related species. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-11-03 Evelise Bach,Camila Gazolla Volpiano,Fernando Hayashi Sant'Anna,Luciane Maria Pereira Passaglia
The taxonomy of Burkholderia sensu lato (s.l.) has been revisited using genome-based tools, which have helped differentiate closely related species. Many species from this group are indistinguishable through phenotypic traits and 16S rRNA gene sequence analysis. Furthermore, they also exhibit whole-genome Average Nucleotide Identity (ANI) values in the twilight zone for species circumscription (95-96%)
-
Exploring the diversity of AVPR2 in Primates and its evolutionary implications. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-11-03 Bibiana Sampaio de Oliveira Fam,Pedro Vargas-Pinilla,Pâmela Paré,Luane Landau,Lucas H Viscardi,Alcides Pissinatti,Tiago Falótico,Renan Maestri,Maria Cátira Bortolini
The current study focuses on the investigation of AVPR2 (VTR2C) protein-coupled receptor variants specific to different primate taxa. AVPR2 is activated by the neurohormone AVP, which modulates physiological processes, including water homeostasis. Our findings reveal positive selection at three AVPR2 sites at positions 190, 250, and 346. Variation at position 250 is associated with human Congenital
-
Disentangling the causes of high polymorphism sharing in sympatric Petunia species from subtropical highland grasslands: insights from nuclear diversity. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-10-30 Luize Simon,Luana S Soares,Loreta B Freitas
Genetic polymorphism sharing between closely related and sympatric plant species could result from common ancestry, ancient or recent hybridization. Here we analyzed four Petunia species from the subtropical highland grasslands in southern South America based on nuclear diversity to disentangle the causes of high polymorphism sharing between them. We genotyped microsatellite loci, employed population
-
Capra hircus outliers markers in Brazil: Searching for genomic regions under the action of natural selection. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-10-20 Francisco de A Diniz Sobrinho,Jeane de O Moura,Miklos M Bajay,Leonardo Castelo Branco,Roosevelt D S Bezerra,Adriana M de Araújo
Identifying genome regions subject to selection in local breeds of Brazil is an opportunity to elucidate the C. hircus genome plasticity, flowing the colonization history of the country. Using SNP Bead Chip Illumina 50K genotyping of Brazilian Anglo-Nubian (standardized breed) and Marota (local endangered population from the semiarid area of Brazil) to show outliers loci in genome regions subject to
-
Unstable environment of coastal lagoons drives genetic variation in the amphipod Quadrivisio lutzi. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-10-13 Mariana Sampaio Xavier,Paulo Cesar Paiva,Laura Isabel Weber
The freshwater/brackish amphipod Quadrivisio lutzi inhabits coastal lagoons, highly unstable environments subject to sudden inflow of marine water. Our aim was to evaluate how the genetic composition varies in these populations. Brazilian populations were compared by 16S rRNA and COI gene sequences. The genetic structure of four Rio de Janeiro amphipod populations was evaluated during the period of
-
Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-10-13 Wen Feng,Lei Zhou,Heng Du,Edward Okoth,Raphael Mrode,Wenjiao Jin,Zhengzheng Hu,Jian-Feng Liu
African swine fever (ASF) is an important viral disease of swine caused by the African swine fever virus (ASFV), which threatens swine production profoundly. To better understand the gene expression changes when pig infected with ASFV, RNA sequencing was performed to characterize differentially expressed genes (DEGs) of six tissues from Kenya domestic pigs and Landrace × Yorkshire (L/Y) pigs infected
-
Chromosomal Radiation: A model to explain karyotypic diversity in cryptic species. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-09-25 Karine Frehner Kavalco,Rubens Pasa
We present a concept that explains the pattern of occurrence of widely distributed organisms with large chromosomal diversity, large or small molecular divergence, and the insufficiency or absence of morphological identity. Our model is based on cytogenetic studies associated with molecular and biological data and can be applied to any lineage of sister species, chronospecies, or cryptic species. Through
-
KDELR2 as a diagnostic and prognostic biomarker of bladder urothelial carcinoma and its correlation with immune infiltration. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-09-25 Sai Ma,Longqi Sa,Jitao Zhang,Kuo Jiang,Baoguo Mi,Lequn Shan
KDELR2 has been reported as a promotive factor for the genesis and progression of several malignancies. However, it is uncertain how it affects bladder urothelial carcinoma (BLCA). Using data extracted from online databases, an enhanced expression of KDELR2 in BLCA tissues was verified. Overexpression of KDELR2 was correlated with advanced clinicopathologic characteristics and unfavourable prognosis
-
Effects on gene expression during maize-Azospirillum interaction in the presence of a plant-specific inhibitor of indole-3-acetic acid production. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-09-18 Eliandro Espindula,Edilena Reis Sperb,Brenda Mor,Vânia Carla Silva Pankievicz,Thalita Regina Tuleski,Michelle Zibetti Tadra-Sfeir,Paloma Bonato,Camila Scheid,Josias Merib,Emanuel Maltempi de Souza,Luciane Maria Pereira Passaglia
Amongst the sustainable alternatives to increase maize production is the use of plant growth-promoting bacteria (PGPB). Azospirillum brasilense is one of the most well-known PGPB being able to fix nitrogen and produce phytohormones, especially indole-3-acetic acid - IAA. This work investigated if there is any contribution of the bacterium to the plant's IAA levels, and how it affects the plant. To
-
Multi-level toxicity assessment of the antidepressant venlafaxine in embryos/larvae and adults of zebrafish (Danio rerio). Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-09-08 Ana Clara Oliveira,Maria Luiza Fascineli,Paula Martins de Oliveira,Guilherme Martins Gelfuso,Rolando André Rios Villacis,Cesar Koppe Grisolia
The toxic effects of venlafaxine (VLX) on aquatic organisms have already been verified and therefore are a proven matter of concern. Herein, we evaluated zebrafish embryos/adults after acute exposure to VLX. Embryos/larvae were exposed to different concentrations of VLX (100-1000 mg/L; 1.33 as a dilution factor), to evaluate mortality/developmental changos and to analyze biomarkers (0.002-100 mg/L)
-
Enhancer in cancer pathogenesis and treatment. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-08-04 Zhuo Sun,Jinbo Fan,Yixiong Dang,Yufeng Zhao
Enhancers are essential cis-acting regulatory elements that determine cell identity and tumor progression. Enhancer function is dependent on the physical interaction between the enhancer and its target promoter inside its local chromatin environment. Enhancer reprogramming is an important mechanism in cancer pathogenesis and can be driven by both cis and trans factors. Super enhancers are acquired
-
Repeated evolution of similar phenotypes: Integrating comparative methods with developmental pathways. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-07-14 Anieli Guirro Pereira,Tiana Kohlsdorf
Repeated phenotypes, often referred to as 'homoplasies' in cladistic analyses, may evolve through changes in developmental processes. Genetic bases of recurrent evolution gained attention and have been studied in the past years using approaches that combine modern analytical phylogenetic tools with the stunning assemblage of new information on developmental mechanisms. In this review, we evaluated
-
Phylogeography and karyotypic evolution of some Deuterodon species from southeastern Brazil (Characiformes, Characidae, Stethaprioninae). Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-07-10 Igor Henrique Rodrigues-Oliveira,Pierre Rafael Penteado,Rubens Pasa,Fabiano Bezerra Menegídio,Karine Frehner Kavalco
Deuterodon is a genus of the subfamily Stethaprioninae, a group of Neotropical fish known as tetras. Deuterodon hastatus represents a species complex, which is supported by cytogenetic and molecular data. In this study, we show the results of comparative evolutionary analyses of the ATP synthase subunit 6 gene in four Deuterodon species, in addition to ribosomal markers (18S rDNA and 5S rDNA), of a
-
The rs1800469 T/T and rs1800470 C/C genotypes of the TGFB1 gene confer protection against diabetic retinopathy in a Southern Brazilian population. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-07-07 Aline Rodrigues Costa,Cristine Dieter,Luís Henrique Canani,Taís Silveira Assmann,Daisy Crispim
The transforming growth factor beta 1 (TGFB1) is a pro-inflammatory cytokine that plays a key role in the mechanisms of angiogenesis and breakdown of the blood-retina barrier, which are implicated in the pathogenesis of diabetic retinopathy (DR). Polymorphisms in the TGFB1 gene have been associated with DR; however, results are still contradictory. Therefore, the aim of this study was to investigate
-
Reduced NCK1 participates in unexplained recurrent miscarriage by regulating trophoblast functions and macrophage proliferation at maternal-fetal interface. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-06-23 Chuanfeng Ding,Donghai Zhang,Shihua Bao,Xin Zhao,Yongsheng Yu,Qian Zhou
Recurrent miscarriage (RM) seriously affects the physical and mental health of women of childbearing age, and 50% of the causes are unknown. Thus, it is valuable to investigate the causes of unexplained recurrent miscarriage (uRM). Similarities between tumor development and embryo implantation make us realize that tumor studies are informative for uRM. The non-catalytic region of tyrosine kinase adaptor
-
Epigenomic integrative analysis pinpoint master regulator transcription factors associated with tumorigenesis in squamous cell carcinoma of oral tongue. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-06-19 Larissa Miyuki Okano,Lívia Maria Maciel da Fonseca,Isabela Dias Erthal,Tathiane Maistro Malta
Head and Neck Cancer (HNC) is a heterogeneous group of cancers, which includes cancers arising in the oral cavity, nasopharynx, oropharynx, hypopharynx, and larynx. Epidemiological studies have revealed that several factors such as tobacco and alcohol use, exposure to environmental pollutants, viral infection, and genetic factors are risk factors for developing HNC. The squamous cell carcinoma of oral
-
TMEM147 is a novel biomarker for diagnosis and prognosis of hepatocellular carcinoma. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-06-16 Wen-Jie Fan,Meng-Xi Zhou,Di-Di Wang,Xin-Xin Jiang,Hao Ding
Hepatocellular carcinoma (HCC) is the most common type of liver malignancy with high incidence and poor prognosis. Transmembrane protein 147 (TMEM147) has been implicated in the development of colon cancer. However, the role of TMEM147 in HCC remains unclear. In this study, data of 371 HCC tissues, 50 adjacent nontumor tissues, and 110 normal liver tissues were retrieved from the TCGA and GTEx databases
-
Genetic diversity of Moroccan date palm revealed by microsatellite markers. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-06-05 Siham Khoulassa,Haris Khurshid,Mohamed Fokar,Hicham Eliddrissy,Youssef Outeha,Jamal Elfadil,Adil Essarioui,Mohamed Benlyas,Rachid Mentag,Benaissa Elmoualij
The genetic diversity between 23 Moroccan date palm cultivars collected from the National Palm Collection at the INRA (National Agricultural Research Institute) experimental field in Zagora was assessed using SSR markers that are specifically designed for date palm. Among the 16 tested SSR, 13 were successfully amplified, and were selected to carry out this study. 208 bands were amplified, ranging
-
The lncRNA MALAT1 is upregulated in urine of type 1 diabetes mellitus patients with diabetic kidney disease. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-06-02 Cristine Dieter,Natália Emerim Lemos,Eliandra Girardi,Denise Taurino Ramos,Nathalia Rodrigues de Faria Corrêa,Luís Henrique Canani,Andrea Carla Bauer,Taís Silveira Assmann,Daisy Crispim
Long non-coding RNAs (lncRNAs) are RNAs with >200 nucleotides that are unable to encode proteins and are involved in gene expression regulation. LncRNAs have a key role in many physiological and pathological processes and, consequently, they have been associated with several human diseases, including diabetes chronic complications, such as diabetes kidney disease (DKD). In this context, some studies
-
The complete chloroplast genome sequence of Vincetoxicum mongolicum (Apocynaceae), a perennial medicinal herb. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-06-02 Wangsuo Liu,Zhanjun Wang,Ying Tian,Bo Ji
Vincetoxicum mongolicum Maxim. (1876), is a perennial medicinal herb, widely distributed in the Loess Plateau of China. Here, we sequenced, assembled, and annotated the complete chloroplast (cp) genome of V. mongolicum, and compared the highly variable gene regions and phylogenetic positions between V. mongolicum and other related species. Results showed that the complete cp genome of V. mongolicum
-
LncRNA AK001796 promotes cell proliferation via acting as a ceRNA of miR-150 in hepatocellular carcinoma. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-06-02 Rui Xu,Haitao He,Yue Wang,Qi Peng,Ke Mei,Yan Liu,Qing Yang
Long non-coding RNA AK001796 was initially identified altered in lung cancer. Recent research showed it could participate in the prognosis of hepatocellular carcinoma (HCC). However, the general biological role of AK001796 and its underlying mechanisms in HCC remain unclear. Here we demonstrated that the expression level of AK001796 in HCC tissues and cell lines was up-regulated. Silencing AK001796
-
Hereditary hemochromatosis beyond hyperferritinemia: Clinical and laboratory investigation of the patient's profile submitted to phlebotomy in two reference centers in southern Brazil. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-05-22 Nathalia Kersting,Juliana Cristine Fontana,Fabiane Pohlmann de Athayde,Fernanda Marcante Carlotto,Bruna Accorsi Machado,Cristiane da Silva Rodrigues de Araújo,Leo Sekine,Tor Gunnar Hugo Onsten,Sandra Leistner-Segal
Hereditary Hemochromatosis is a disorder characterized by iron deposition in several organs and hyperferritinemia. The most studied variants are linked to the HFE gene. In Brazil, surveys that characterize this population are scarce, with no sampling in the state of Rio Grande do Sul. Our objective is to carry out a data collection focusing on the profile of this population and the influence of the
-
Chromosome diversity in Buthidae and Chactidae scorpions from Brazilian fauna: Diploid number and distribution of repetitive DNA sequences. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-05-15 Juliana Figueiredo Lima,Leonardo Sousa Carvalho,Marcos André Carvalho,Marielle Cristina Schneider
In this work, we analyzed cytogenetically eight Chactidae and Buthidae, including the localization of repetitive DNA sequences. The chactids possess monocentric chromosomes and the highest diploid numbers (2n=50 in Brotheas amazonicus, 2n=36 in Chactopsis amazonica, 2n=30 in Neochactas sp.) when compared with buthids (2n=10 in Tityus bahiensis, 2n=14 in Tityus apiacas and Tityus metuendus, 2n=18 in
-
A germline-targeted genetic screen for xrn-2 suppressors identifies a novel gene C34C12.2 in Caenorhabditis elegans. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-05-15 Ilkin Aygün,Alicja Rzepczak,Takashi S Miki
XRN2 is an evolutionarily conserved 5'-to-3' exoribonuclease, which degrades or trims various types of RNA in the nucleus. Although XRN-2 is essential for embryogenesis, larval development and reproduction in Caenorhabditis elegans, relevant molecular pathways remain unidentified. Here we create a germline-specific xrn-2 conditional mutant and perform a mutagenesis screen for suppressors of sterility
-
Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice (Oryza sativa L.) ionome. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-05-05 Andriele Wairich,Ariane Vitali,Janete Mariza Adamski,Karina Letícia Lopes,Guilherme Leitão Duarte,Lucas Roani Ponte,Henrique Keller Costa,Paloma Koprovski Menguer,Rinaldo Pires Dos Santos,Janette Palma Fett,Raul Antonio Sperotto,Felipe Klein Ricachenevsky
NAC transcription factors are plant-specific proteins involved in many processes during the plant life cycle and responses to biotic and abiotic stresses. Previous studies have shown that stress-induced OsNAC5 from rice (Oryza sativa L.) is up-regulated by senescence and might be involved in control of iron (Fe) and zinc (Zn) concentrations in rice seeds. Aiming a better understanding of the role of
-
Genetic diversity of toxigenic Fusarium verticillioides associated with maize grains, India. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-04-07 Vishwambar D Navale,Amol M Sawant,Koteswara Rao Vamkudoth
In the present investigation, prevalence, genetic diversity, and mycotoxin producing potential of Fusarium species associated with maize grain samples were studied from different geographical regions of India. The highest prevalence of Fusarium verticillioides was recorded as 88.52%, followed by F. coffeatum, F. foetens, and F. euwallaceae, 6.55%, 3.27%, and 1.63%, respectively. We isolated 54 strains
-
Sampling strategies for sugarcane using either clonal replicates or diverse genotypes can bias the conclusions of RNA-Seq studies. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-04-03 Victor Hugo Mello,Ana Letycia Basso Garcia,Fernando Henrique Correr,Guilherme Kenichi Hosaka,Monalisa Sampaio Carneiro,Gabriel Rodrigues Alves Margarido
A key procedure for ensuring statistical confidence in differential gene expression analyses is to use biological replicates to compare distinct groups. Biological replicates allow the estimation of the residual variation in the gene expression levels among samples of a given experimental condition. In sugarcane, it is possible to obtain an estimate of residual variability at two levels: among samples
-
Wheat blast: The last enemy of hunger fighters. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-04-03 Valeria Oliveira Nizolli,Vívian Ebeling Viana,Camila Pegoraro,Luciano Carlos da Maia,Antonio Costa de Oliveira
Effective strategies for disease control are crucial for sustaining world food production and ensuring food security for the population. Wheat blast, a disease caused by the pathogen Magnaporthe oryzae pathotype Triticum, has been a concern for cereal producers and researchers due to its aggressiveness and rapid expansion. To solve this problem, the development of resistant varieties with durable resistance
-
Exosomes secreted from bone marrow mesenchymal stem cells suppress cardiomyocyte hypertrophy through Hippo-YAP pathway in heart failure. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-03-13 Yu Ren,Yun Wu,Wenshuai He,Yingjie Tian,Xingsheng Zhao
Mesenchymal stem cells-derived exosomes (MSCs-exosomes) reportedly possess cardioprotective effects. This study investigated the therapeutic potential and mechanisms of MSCs-exosomes on heart failure (HF). H9c2 cells were used to establish a cardiomyocyte hypertrophy model by angiotensin II (Ang II) treatment. Isolated MSCs-exosomes were identified by transmission electron microscope and CD63 detection
-
Intercepting biological messages: Antibacterial molecules targeting nucleic acids during interbacterial conflicts. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-03-06 Julia Takuno Hespanhol,Lior Karman,Daniel Enrique Sanchez-Limache,Ethel Bayer-Santos
Bacteria live in polymicrobial communities and constantly compete for resources. These organisms have evolved an array of antibacterial weapons to inhibit the growth or kill competitors. The arsenal comprises antibiotics, bacteriocins, and contact-dependent effectors that are either secreted in the medium or directly translocated into target cells. During bacterial antagonistic encounters, several
-
Genome editing in maize: Toward improving complex traits in a global crop. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-03-03 José Hernandes-Lopes,Juliana Erika de Carvalho Teixeira Yassitepe,Alessandra Koltun,Laurens Pauwels,Viviane Cristina Heinzen da Silva,Ricardo Augusto Dante,Isabel Rodrigues Gerhardt,Paulo Arruda
Recent advances in genome editing have enormously enhanced the effort to develop biotechnology crops for more sustainable food production. CRISPR/Cas, the most versatile genome-editing tool, has shown the potential to create genome modifications that range from gene knockout and gene expression pattern modulations to allele-specific changes in order to design superior genotypes harboring multiple improved
-
Vitamin D and estrogen steroid hormones and their immunogenetic roles in Infectious respiratory (TB and COVID-19) diseases. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-02-06 Maria Eduarda de Albuquerque Borborema,Thays Maria Costa de Lucena,Jaqueline de Azevêdo Silva
The role of steroid hormones against infectious diseases has been extensively studied. From immunomodulatory action to direct inhibition of microorganism growth, hormones D3 (VD3) and 17β-estradiol (E2), and the genetic pathways modulated by them, are key targets for a better understanding pathogenesis of infectious respiratory diseases (IRD) such as tuberculosis (TB) and the coronavirus disease-19
-
Niclosamide is a potential candidate for the treatment of chemo-resistant osteosarcoma. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-02-03 Xiaoling Huang,Wei Wang,Yong Li
Chemotherapy is the main treatment option for advanced osteosarcoma, which is the most common type of primary bone malignancy. However, patients develop resistance rapidly and many succumb to the disease. Niclosamide, an anthelmintic drug, has been recently identified to display potent and selective anti-cancer activity. In this work, we show that niclosamide at sub-micromolar concentrations inhibits
-
Interchromosomal translocation in neural progenitor cells exposed to L1 retrotransposition. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-01-30 Alysson R Muotri
LINE-1 (L1) elements are a class of transposons, comprising approximately 19% and 21% of the mouse and human genomes, respectively. L1 retrotransposons can reverse transcribe their own RNA sequence into a de novo DNA copy integrated into a new genomic location. This activity, known as retrotransposition, may induce genomic alterations, such as insertions and deletions. Interestingly, L1s can retrotranspose
-
Molecular identification of Austrodiplostomum sp., an eye parasite among farmed tambaqui in Amazonia. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-01-30 Eduardo Freitas de Farias,Hallana Cristina Menezes da Silva,Ana Paula Costa de Carvalho,Rodrigo de Melo Martins,Andrea Belem-Costa,Wallice Paxiúba Duncan,Ana Lúcia Silva Gomes,Daniele Aparecida Matoso
Austrodiplostomum Szidat & Nani, 1951 is a genus of parasitic digenetic trematodes widely distributed in the Neotropical region. Infects a wide variety of species, families and requests for freshwater fish. We identify samples of Austrodiplostomum sp, based on metacercariae isolates from eyes of tambaqui (Colossoma macropomum), a fish of high commercial importance in Brazil, and widely consumed by
-
Mutations and insights into the molecular mechanisms of resistance of Mycobacterium tuberculosis to first-line. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-01-23 Nicolas de Oliveira Rossini,Marcio Vinicius Bertacine Dias
Genetically antimicrobial resistance in Mycobacterium tuberculosis is currently one of the most important aspects of tuberculosis, considering that there are emerging resistant strains for almost every known drug used for its treatment. There are multiple antimicrobials used for tuberculosis treatment, and the most effective ones are the first-line drugs, which include isoniazid, pyrazinamide, rifampicin
-
Beyond transcription factors: more regulatory layers affecting soybean gene expression under abiotic stress. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-01-23 Isabel Cristina Cadavid,Natalia Balbinott,Rogerio Margis
Abiotic stresses such as nutritional imbalance, salt, light intensity, and high and low temperatures negatively affect plant growth and development. Through the course of evolution, plants developed multiple mechanisms to cope with environmental variations, such as physiological, morphological, and molecular adaptations. Epigenetic regulation, transcription factor activity, and post-transcriptional
-
Transcribed Ultraconserved Regions: New regulators in cancer signaling and potential biomarkers. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-01-09 Jaqueline Carvalho de Oliveira
The ultraconserved regions (UCRs) are 481 genomic elements, longer than 200 bp, 100% conserved in human, mouse, and rat genomes. Usually, coding regions are more conserved, but more than 80% of UCRs are either intergenic or intronic, and many of them produce long non-coding RNAs (lncRNAs). Recently, the deregulated expression of transcribed UCRs (T-UCRs) has been associated with pathological conditions
-
Comparative cytogenetics among Boana species (Anura, Hylidae): focus on evolutionary variability of repetitive DNA. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-01-06 Sebastião Venancio Neto,Rafael Bueno Noleto,Matheus Azambuja,Camilla Borges Gazolla,Bianca Rocha Santos,Viviane Nogaroto,Marcelo Ricardo Vicari
Boana comprises a diverse genus of Neotropical treefrogs, currently rearranged into seven taxonomic species groups. Although cytogenetic studies have demonstrated diversity in its representatives, the chromosomal mapping of repetitive DNA sequences is still scarce. In this study, Boana albopunctata, Boana faber, and Boana prasina were subjected to in situ localization of different repetitive DNA units
-
Systematically analyzed molecular characteristics of lung adenocarcinoma using metabolism-related genes classification. Genet. Mol. Biol. (IF 2.1) Pub Date : 2023-01-06 Xiaoming Huang,Feng Zhang,Junqi Lin,Shaoming Lin,Guanle Shen,Xiaozhu Chen,Wenbiao Chen
High heterogeneity of lung adenocarcinoma (LUAD) is a major clinical challenge. This study aims to characterize the molecular features of LUAD through classification based on metabolism-related genes. A total of 500 LUAD samples from The Cancer Genome Atlas (TCGA) and 612 from Gene Expression Omnibus (GEO) were integrated with 2,753 metabolism-related genes to determine the molecular classification
-
Molecular evolution and diversification of phytoene synthase (PSY) gene family. Genet. Mol. Biol. (IF 2.1) Pub Date : 2022-12-19 Marcia Pagno Lisboa,Drielli Canal,João Pedro Carmo Filgueiras,Andreia Carina Turchetto-Zolet
Phytoene synthase (PSY) is a crucial enzyme required for carotenoid biosynthesis, encoded by a gene family conserved in carotenoid-producing organisms. This gene family is diversified in angiosperms through distinct duplication events. Understanding diversification patterns and the evolutionary history of the PSY gene family is important for explaining carotenogenesis in different plant tissues. This
-
Protective role of exosomes derived from regulatory T cells against inflammation and apoptosis of BV-2 microglia under oxygen-glucose deprivation/reperfusion challenge. Genet. Mol. Biol. (IF 2.1) Pub Date : 2022-12-19 Changqing Yang,Fei Yuan,Wan Shao,Lihong Yao,Shaoju Jin,Fangfang Han
Regulatory T cells (Tregs) are found to participate in the pathogenesis of cerebral ischemic stroke. Exosomes derived from Tregs (Treg-Exos) were found to mediate the mechanism of Tregs' roles under various physiological and pathological conditions. But the roles of Treg-Exos in cerebral ischemic stroke are still unclear. Here, we explored the protective effects of Treg-Exos against microglial injury
-
Evolutionary and structural aspects of Solanaceae RNases T2. Genet. Mol. Biol. (IF 2.1) Pub Date : 2022-12-16 Claudia Elizabeth Thompson,Lauís Brisolara-Corrêa,Helen Nathalia Thompson,Hubert Stassen,Loreta Brandão de Freitas
Plant RNases T2 are involved in several physiological and developmental processes, including inorganic phosphate starvation, senescence, wounding, defense against pathogens, and the self-incompatibility system. Solanaceae RNases form three main clades, one composed exclusively of S-RNases and two that include S-like RNases. We identified several positively selected amino acids located in highly flexible
-
Molecular evolution and structural analyses of proteins involved in metabolic pathways of volatile organic compounds in Petunia hybrida (Solanaceae). Genet. Mol. Biol. (IF 2.1) Pub Date : 2022-12-16 Lucas C Beltrame,Claudia E Thompson,Loreta B Freitas
The association between plants and their pollinators is essential for increasing the diversity in angiosperms. Morphological and physiological traits, mainly floral scent, can influence the pollination dynamics and select pollinators for each plant species. In this work, we studied two proteins involved in producing volatile organic compounds in plants, conyferyl alcohol acyltransferase (CFAT) and
-
Sugarcane multitrophic interactions: Integrating belowground and aboveground organisms. Genet. Mol. Biol. (IF 2.1) Pub Date : 2022-12-09 Diego Z Gallan,Augusto B Penteriche,Maressa O Henrique,Marcio C Silva-Filho
Sugarcane is a crop of major importance used mainly for sugar and biofuel production, and many additional applications of its byproducts are being developed. Sugarcane cultivation is plagued by many insect pests and pathogens that reduce sugarcane yields overall. Recently emerging studies have shown complex multitrophic interactions in cultivated areas, such as the induction of sugarcane defense-related
-
Genome-wide, evolutionary, and functional analyses of ascorbate peroxidase (APX) family in Poaceae species. Genet. Mol. Biol. (IF 2.1) Pub Date : 2022-12-09 Douglas Jardim-Messeder,Andreia Caverzan,Gabriel Afonso Bastos,Vanessa Galhego,Ygor de Souza-Vieira,Fernanda Lazzarotto,Esther Felix-Mendes,Lucas Lavaquial,José Nicomedes Junior,Márcia Margis-Pinheiro,Gilberto Sachetto-Martins
Ascorbate peroxidases (APXs) are heme peroxidases involved in the control of hydrogen peroxide levels and signal transduction pathways related to development and stress responses. Here, a total of 238 APX, 30 APX-related (APX-R), and 34 APX-like (APX-L) genes were identified from 24 species from the Poaceae family. Phylogenetic analysis of APX indicated five distinct clades, equivalent to cytosolic
-
Functional analysis of alternative castor bean DGAT enzymes. Genet. Mol. Biol. (IF 2.1) Pub Date : 2022-12-09 Thomaz Stumpf Trenz,Andreia Carina Turchetto-Zolet,Rogério Margis,Marcia Margis-Pinheiro,Felipe Dos Santos Maraschin
The diversity of diacylglycerol acyltransferases (DGATs) indicates alternative roles for these enzymes in plant metabolism besides triacylglycerol (TAG) biosynthesis. In this work, we functionally characterized castor bean (Ricinus communis L.) DGATs assessing their subcellular localization, expression in seeds, capacity to restore triacylglycerol (TAG) biosynthesis in mutant yeast and evaluating whether
-
A phylogenetic review of cancer resistance highlights evolutionary solutions to Peto's Paradox. Genet. Mol. Biol. (IF 2.1) Pub Date : 2022-12-05 Mariana F Nery,Mathias Rennó,Agnello Picorelli,Elisa Ramos
Cancer is a genetic disease present in all complex multicellular lineages. Finding ways to eliminate it is a goal of a large part of the scientific community and nature itself. Early, scientists realized that the cancer incidence at the species level was not related to the number of cells or lifespan, a phenomenon called Peto's Paradox. The interest in resolving this paradox triggered a growing interest
-
Comparative genomics and phylogenomics of the Ralstonia solanacearum Moko ecotype and its symptomatological variants. Genet. Mol. Biol. (IF 2.1) Pub Date : 2022-12-02 Ana Karolina Leite Pais,Leandro Victor Silva Dos Santos,Greecy Mirian Rodrigues Albuquerque,Antonio Roberto Gomes de Farias,Wilson José Silva Junior,Valdir de Queiroz Balbino,Adriano Márcio Freire Silva,Marco Aurelio Siqueira da Gama,Elineide Barbosa de Souza
Banana tree bacterial wilt is caused by the Ralstonia solanacearum Moko ecotype. These strains vary in their symptom progression in banana, and are classified as typical Moko variants (phylotype IIA and IIB strains from across Central and South America), Bugtok variant (Philippines), and Sergipe facies (the states of Sergipe and Alagoas, Brazil). This study used comparative genomic and phylogenomic
-
Telomere organization and the interstitial telomeric sites involvement in insects and vertebrates chromosome evolution. Genet. Mol. Biol. (IF 2.1) Pub Date : 2022-11-14 Marcelo Ricardo Vicari,Daniel Pacheco Bruschi,Diogo Cavalcanti Cabral-de-Mello,Viviane Nogaroto
Telomere has a central role in chromosomal stability events. Chromosome ends organized in telomere-loop prevent activation of DNA damage response (DDR) mechanisms, thus keeping the chromosome structure organized. On the other hand, free chromosome ends, dysfunctional telomeres, and interstitial telomeric sequences (ITS) can trigger chromosome rearrangements. Here, the telomere organization, function
-
The complete plastid genome of Bactris riparia (Arecaceae) and a comparative analysis in Bactridinae (Cocoseae, Arecaceae). Genet. Mol. Biol. (IF 2.1) Pub Date : 2022-11-07 Micheli Cristina Dias,Charles Roland Clement,Hugo Pacheco de Freitas Fraga,Raquel Santos da Silva,Doriane Picanço Rodrigues,Leila do Nascimento Vieira
Here we sequenced and characterized the complete plastome of Bactris riparia, a species closely related to B. gasipaes and widely distributed in Western Amazonia. We performed a comparative genomic analysis with B. riparia and the other four Bactridinae species retrieved from GenBank. The plastome of B. riparia was 156,715 bp with a quadripartite structure. Gene content included 86 protein-coding genes
-
Association of PSORS1C3, CARD14 and TLR4 genotypes and haplotypes with psoriasis susceptibility. Genet. Mol. Biol. (IF 2.1) Pub Date : 2022-11-07 Nguyen Thi Thuy Linh,Nguyen Hoang Giang,Nguyen Thi Kim Lien,Bui Kieu Trang,Do Thi Trang,Nguyen Thy Ngoc,Vu Xuan Nghia,Le Tra My,Can Van Mao,Nguyen Huy Hoang,Nguyen Thi Xuan
Psoriasis is a common chronic, immune-mediated inflammatory disease of the skin. PSORS1C3 is a non-protein coding gene, of which the RNA transcript is found in psoriatic patients. CARD14 is mainly expressed in epidermal keratinocytes. TLR4 is a transmembrane protein to recognize microbial antigens. Our study aimed to assess the relationship among PSORS1C3, CARD14 and TLR4 polymorphisms, inflammatory
-
DNA repair-related genes and adipogenesis: Lessons from congenital lipodystrophies. Genet. Mol. Biol. (IF 2.1) Pub Date : 2022-11-07 Julliane Tamara Araújo de Melo Campos,Matheus Sena de Oliveira,Luisa Pessoa Soares,Katarina Azevedo de Medeiros,Leonardo René Dos Santos Campos,Josivan Gomes Lima
Classical and progeroid congenital lipodystrophies are a collection of rare diseases displaying a large genetic heterogeneity. They occur due to pathogenic variants in genes associated with adipogenesis, DNA repair pathways, and genome stability. Subjects with lipodystrophy exhibit an impairment in the homeostasis of subcutaneous white adipose tissue (sWAT), resulting in low leptin and adiponectin
-
Prevalence and molecular characterization of alpha and beta-Thalassemia mutations among Hakka people in southern China. Genet. Mol. Biol. (IF 2.1) Pub Date : 2022-10-24 XiangXing Zeng,ZhiFang Liu,CaiHua He,Jia Wang,LiXiang Yan
Our aim was to investigate molecular features of thalassemia for proper clinical consultation and prevention in Heyuan. In our research, a total of 25,437 positive screening subjects were further subjected to a genetic analysis of α-thalassemia (α-thal) and β-thalassemia (β-thal). The deletion of α-thal mutation was tested by Gap-PCR, while the non-deletion of α-thal and β-thal mutation were identified
-
Low STR variability in the threatened marsh deer, Blastocerus dichotomus, detected through amplicon sequencing in non-invasive samples. Genet. Mol. Biol. (IF 2.1) Pub Date : 2022-10-24 Laura Irene Wolfenson,Gregory R McCracken,Daniel E Ruzzante,Patricia Mirol,Antonio Solé-Cava
Blastocerus dichotomus is the largest deer in South America. We have used 25 microsatellite markers detected and genotyped by Next Generation Sequencing to estimate the genetic variability of B. dichotomus in Argentina, where most of its populations are threatened. Primer design was based on the sequence of a shallow partial genome (15,967,456 reads; 16.66% genome coverage, mean depth 1.64) of a single