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A re-examination of the West European species of Boreonectes Angus, 2010, with particular reference to B. multilineatus (Falkenström, 1922) (Coleoptera, Dytiscidae) Comp. Cytogenet. (IF 1.036) Pub Date : 2021-01-14 Robert B. Angus
The West European species of Boreonectes Angus, 2010 are reviewed. B. multilineatus (Falkenström, 1922) is shown to be widely distributed in the Pyrenees, where it is the only species known to occur. The chromosomes of all five west European species are found to have, in addition their different numbers of chromosomes, differences in the number and locations of secondary constrictions, and in some
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Chromosomal and DNA barcode analysis of the Polyommatus (Agrodiaetus) damone (Eversmann, 1841) species complex (Lepidoptera, Lycaenidae) Comp. Cytogenet. (IF 1.036) Pub Date : 2021-01-04 Vladimir A. Lukhtanov; Alexander V. Dantchenko
The Polyommatus (Agrodiaetus) damone (Eversmann, 1841) species complex comprises from 5 to 8 species distributed in southeastern Europe and southern Siberia. Here we used chromosomal and DNA-barcode markers in order to test the taxonomic hypotheses previously suggested for this complex. We revealed that all taxa within this group demonstrate chromosomal stasis and share the same or very similar haploid
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Corrigenda: Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907. Comparative Cytogenetics 14(2): 231–242. https://doi.org/10.3897/CompCytogen.v14i2.49513 Comp. Cytogenet. (IF 1.036) Pub Date : 2020-12-29 Caio Augusto Gomes Goes; Sandro Natal Daniel; Lucas Henrique Piva; George Shigueki Yasui; Roberto Ferreira Artoni; Diogo Teruo Hashimoto; Fausto Foresti; Fábio Porto-Foresti
Astyanax Baird et Girard, 1854, is one of the largest genera in the family Characidae and comprises 177 valid species. This genus has been the focus of cytogenetic studies primarily owing to the presence of B chromosomes and high karyotypic diversity among different populations. The intense genetic variability in Astyanax is one of the factors responsible for the occurrence of species complexes, which
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Detection of cryptic diversity in lizards (Squamata) from two Biosphere Reserves in Mesoamerica Comp. Cytogenet. (IF 1.036) Pub Date : 2020-12-22 Riccardo Castiglia; Oscar Alberto Flores-Villela; Alexandra M. R. Bezerra; Ekaterina Gornung; Flavia Annesi; Luis Antonio Muñoz-Alonso; Emanuela Solano
A combined approach based on karyology and DNA taxonomy allowed us to characterize the taxonomic peculiarities in 10 Mesoamerican lizard species, belonging to six genera and five families, inhabiting two Biosphere Reserve in Chiapas, Mexico: La Sepultura Biosphere Reserve, and Montes Azules Biosphere. The karyotypes of four species, Phyllodactylus sp. 3 (P. tuberculosus species group) (2n = 38), Holcosus
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Comparative karyotype study of three Cyprinids (Cyprinidae, Cyprininae) in Thailand by classical cytogenetic and FISH techniques Comp. Cytogenet. (IF 1.036) Pub Date : 2020-12-22 Sumalee Phimphan; Patcharaporn Chaiyasan; Chatmongkon Suwannapoom; Montri Reungsing; Sippakorn Juntaree; Alongklod Tanomtong; Weerayuth Supiwong
Three species of ornamental fishes in the subfamily Cyprininae (family Cyprinidae) namely, Epalzeorhynchos frenatum (Fowler, 1934), Puntigrus partipentazona (Fowler, 1934), Scaphognathops bandanensis Boonyaratpalin et Srirungroj, 1971 were studied by classical cytogenetic and fluorescent in situ hybridization (FISH) techniques. Chromosomes were directly prepared from kidney tissues and stained by using
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Homologous series by Nikolai Vavilov in the phylogeny of Homoptera Comp. Cytogenet. (IF 1.036) Pub Date : 2020-12-17 Ilya A. Gavrilov-Zimin
The paper briefly discusses the most impressive examples of the Nikolai Vavilov’s “Law of homologous series” in the evolution of one of the largest animal groups, homopterous insects, which comprise about 65,000 recent species in the world fauna. Different taxonomic and phylogenetic characters (morpho-anatomical, cytogenetic, reproductive and others) are considered at the taxonomic ranks of the order
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New cytogenetic data for three species of Pentatomidae (Heteroptera): Dichelops melacanthus (Dallas, 1851), Loxa viridis (Palisot de Beauvois, 1805), and Edessa collaris (Dallas, 1851) Comp. Cytogenet. (IF 1.036) Pub Date : 2020-11-17 Jaqueline Fernanda Dionísio; Joana Neres da Cruz Baldissera; Angélica Nunes Tiepo; José Antônio Marin Fernandes; Daniel Ricardo Sosa-Gómez; Renata da Rosa
In this paper, we present new cytogenetic data for three species of the family Pentatomidae: Dichelops melacanthus (Dallas, 1851), Loxa viridis (Palisot de Beauvois, 1805), and Edessa collaris (Dallas, 1851). All studied species presented holocentric chromosomes and inverted meiosis for the sex chromosomes. D. melacanthus has 2n = 12 (10A + XY); L. viridis showed 2n = 14 (12A + XY); and E. collaris
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Karyotype and DNA barcode of Polyommatus (Agrodiaetus) cyaneus (Staudinger, 1899) from its type locality: implication for taxonomic and evolutionary research in Polyommatus blue butterflies (Lepidoptera, Lycaenidae) Comp. Cytogenet. (IF 1.036) Pub Date : 2020-11-17 Vladimir A. Lukhtanov; Alexander V. Dantchenko; Karine V. Balayan; Anastasia V. Gagarina
Chromosomal and molecular analyses of rapidly evolving organisms such as Polyommatus Latreille, 1804 blue butterflies are essential for understanding their taxonomy and evolutionary history, and the studies of populations from their type localities are crucially important for resolving problems of nomenclature and species identity. Here we present data on the topotypical population of the blue butterfly
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Karyotypes diversity in some Iranian Pamphagidae grasshoppers (Orthoptera, Acridoidea, Pamphagidae): new insights on the evolution of the neo-XY sex chromosomes Comp. Cytogenet. (IF 1.036) Pub Date : 2020-11-10 Olesya Buleu; Ilyas Jetybayev; Mohsen Mofidi-Neyestanak; Alexander Bugrov
For the first time, cytogenetic features of grasshoppers from Iran have been studied. In this paper we conducted a comparative cytogenetic analysis of six species from the family Pamphagidae. The species studied belong to subfamilies Thrinchinae Stål, 1876 (Eremopeza bicoloripes (Moritz, 1928), E. saussurei (Uvarov, 1918)) and Pamphaginae (Saxetania paramonovi (Dirsh, 1927), Tropidauchen escalerai
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First cytogenetic information on four checkered beetles (Coleoptera, Cleridae) Comp. Cytogenet. (IF 1.036) Pub Date : 2020-10-27 Atılay Yağmur Okutaner
The karyotypes of four species of Cleridae (Coleoptera): Trichodes favarius (Illiger, 1802), Trichodes quadriguttatus Adams, 1817, Trichodes reichei (Mulsant et Rey, 1863), and Tilloidea transversalis (Charpentier, 1825) were reported for the first time with this study. The chromosome numbers of these four species were determined as 2n = 18, sex chromosome system Xyp, and all chromosomes were metacentric
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A checklist of chromosome numbers and a review of karyotype variation in Odonata of the world Comp. Cytogenet. (IF 1.036) Pub Date : 2020-10-22 Valentina G. Kuznetsova; Natalia V. Golub
The ancient insect order Odonata is divided into three suborders: Anisoptera and Zygoptera with approximately 3000 species worldwide each, and Anisozygoptera with only four extant species in the relict family Epiophlebiidae. An updated list of Odonata species studied regarding chromosome number, sex chromosome mechanism and the occurrence of m-chromosomes (= microchromosomes) is given. Karyotypes of
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DNA-barcoding and a new data about the karyotype of Myotis petax (Chiroptera, Vespertilionidae) in the Russian Far East Comp. Cytogenet. (IF 1.036) Pub Date : 2020-10-19 Uliana V. Gorobeyko; Irina V. Kartavtseva; Irina N. Sheremetyeva; Denis V. Kazakov; Valentin Yu. Guskov
The DNA-barcoding and chromosomal study of the eastern water bat, Myotis petax Hollister, 1912, from the earlier unexplored localities in the Russian Far East are carried out. The COI barcoding obtained for 18 from a total of 19 individuals captured in five localities in the Russian Far East showed the low nucleotide variability with the prevalence of the central, the most abundant haplotype. The chromosomal
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Random chromosome distribution in the first meiosis of F1 disomic substitution line 2R(2D) x rye hybrids (ABDR, 4× = 28) occurs without bipolar spindle assembly Comp. Cytogenet. (IF 1.036) Pub Date : 2020-10-09 Dina B. Loginova; Anastasia A. Zhuravleva; Olga G. Silkova
The assembly of the microtubule-based spindle structure in plant meiosis remains poorly understood compared with our knowledge of mitotic spindle formation. One of the approaches in our understanding of microtubule dynamics is to study spindle assembly in meiosis of amphyhaploids. Using immunostaining with phH3Ser10, CENH3 and α-tubulin-specific antibodies, we studied the chromosome distribution and
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Comparative cytogenetic of six species of Amazonian Peacock bass (Cichla, Cichlinae): intrachromosomal variations and genetic introgression among sympatric species Comp. Cytogenet. (IF 1.036) Pub Date : 2020-09-17 Janice Quadros; Alex M. V. Ferreira; Patrik F. Viana; Leandro Marajó; Ezequiel Oliveira; Efrem Ferreira; Eliana Feldberg
Cytogenetic data for the genus Cichla Bloch et Schneider, 1801 are still very limited, with only four karyotype descriptions to date. The sum of the available cytogenetic information for Cichla species, points to a maintenance of the diploid number of 48 acrocentric chromosomes, considered a typical ancestral feature in cichlids. In the current study, we performed molecular and classical cytogenetic
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Rapid chromosomal evolution in the bush-cricket Gonatoxia helleri Hemp, 2016 (Orthoptera, Phaneropterinae). Comp. Cytogenet. (IF 1.036) Pub Date : 2020-08-28 Elżbieta Warchałowska-Śliwa,Beata Grzywacz,Anna Maryańska-Nadachowska,Klaus-Gerhard Heller,Claudia Hemp
Gonatoxia helleri Hemp, 2016 is one of the most widespread bush-crickets of the genus Gonatoxia Karsch, 1889 in East Africa. This species with seven large chromosomes (2n♂ = 7) differs from other representatives of the genus Gonatoxia drastically by its reduced chromosome number, the asymmetrical karyotype including karyomorphs rarely found in tettigoniids, as well as in irregularities in the course
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Chromosomes of parasitic wasps of the superfamily Chalcidoidea (Hymenoptera): An overview Comp. Cytogenet. (IF 1.036) Pub Date : 2020-08-25 Vladimir E. Gokhman
An overview of the current knowledge of chromosome sets of the parasitoid superfamily Chalcidoidea is given. Karyotypes of approximately 240 members of this group, i.e. just above one percent of described species, are studied up to now. Techniques for obtaining and analyzing preparations of chalcid chromosomes are outlined, including the so-called “traditional” and “modern” methods of differential
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First cytogenetic information for five Nilotic elephantfishes and a problem of ancestral karyotype of the family Mormyridae (Osteoglossiformes). Comp. Cytogenet. (IF 1.036) Pub Date : 2020-08-19 Sergey Simanovsky,Dmitry Medvedev,Fekadu Tefera,Alexander Golubtsov
The elephantfish family Mormyridae is the most diverse lineage of the primitive teleostean clade Osteoglossomorpha distributed in inland waters of all continents except Antarctica and Europe. The family Mormyridae is endemic to Africa and includes 22 genera and almost 230 species. The evolutionary radiation of mormyrids most probably should be attributed to their capability of both generating and receiving
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Comparative FISH-mapping of TTAGG telomeric sequences to the chromosomes of leafcutter ants (Formicidae, Myrmicinae): is the insect canonical sequence conserved? Comp. Cytogenet. (IF 1.036) Pub Date : 2020-08-14 Carini Picardi Morais de Castro,Danon Clemes Cardoso,Ricardo Micolino,Maykon Passos Cristiano
Telomeric sequences are conserved across species. The most common sequence reported among insects is (TTAGG)n, but its universal occurrence is not a consensus because other canonical motifs have been reported. In the present study, we used fluorescence in situ hybridization (FISH) using telomeric probes with (TTAGG)6 repeats to describe the telomere composition of leafcutter ants. We performed the
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The highly rearranged karyotype of the hangingfly Bittacus sinicus (Mecoptera, Bittacidae): the lowest chromosome number in the order. Comp. Cytogenet. (IF 1.036) Pub Date : 2020-07-30 Ying Miao,Bao-Zhen Hua
Cytogenetic features of the hangingfly Bittacus sinicus Issiki, 1931 were investigated for the first time using C-banding and DAPI (4',6-diamidino-2-phenylindole) staining. The karyotype analyses show that the male B. sinicus possesses the lowest chromosome number (2n = 15) ever observed in Mecoptera, and an almost symmetric karyotype with MCA (Mean Centromeric Asymmetry) of 12.55 and CVCL (Coefficient
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Chromosomal and reproductive features of some Oriental and Australasian scale insects (Homoptera, Coccinea). Comp. Cytogenet. (IF 1.036) Pub Date : 2020-07-20 Ilya A Gavrilov-Zimin
Fourteen species of scale insects from the families Margarodidae s.l., Pseudococcidae, Eriococcidae, and Coccidae were investigated for the first time in respect to karyotypes, genetic systems, modes of reproduction and general anatomy of the female reproductive system. One of the studied species, Steatococcus samaraius Morrison, 1927, showed hermaphroditic reproduction of the female-like specimens
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Cytogenetic characterization, rDNA mapping and quantification of the nuclear DNA content in Seriolella violacea Guichenot, 1848 (Perciformes, Centrolophidae). Comp. Cytogenet. (IF 1.036) Pub Date : 2020-07-14 Cristian Araya-Jaime,Claudio Palma-Rojas,Elisabeth Von Brand,Alfonso Silva
Seriolella violacea Guichenot, 1848 is an important component of the fish fauna of the Chilean coast and is of great economic interest. Cytogenetic information for the family Centrolophidae is lacking and the genomic size of five of the twenty-eight species described for this family are is barely known. This study aimed to describe for the first time the karyotype structure via classical and molecular
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Notable homologous variation in chromosomal races of the common shrew. Comp. Cytogenet. (IF 1.036) Pub Date : 2020-07-14 Nina Bulatova
This paper is a review of the rare phenomenon of chromosome intraspecies variation manifested in monobrachial homology series in the comprehensively investigated karyotype of the common shrew Sorex araneus Linnaeus, 1758 (Eulipotyphla, Mammalia). The detailed dataset on the account of this mammalian species was drawn from the recently published monograph by Searle et al. (2019) “Shrews, Chromosomes
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The epigenetic regulation of centromeres and telomeres in plants and animals. Comp. Cytogenet. (IF 1.036) Pub Date : 2020-07-07 Magdalena Achrem,Izabela Szućko,Anna Kalinka
The centromere is a chromosomal region where the kinetochore is formed, which is the attachment point of spindle fibers. Thus, it is responsible for the correct chromosome segregation during cell division. Telomeres protect chromosome ends against enzymatic degradation and fusions, and localize chromosomes in the cell nucleus. For this reason, centromeres and telomeres are parts of each linear chromosome
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Comparative molecular cytogenetic characterization of five wild Vigna species (Fabaceae). Comp. Cytogenet. (IF 1.036) Pub Date : 2020-06-26 Chao-Wen She,Ying Mao,Xiang-Hui Jiang,Chun-Ping He
To extend our knowledge on karyotype variation of the genus Vigna Savi, 1824, the chromosomal organization of rRNA genes and fluorochrome banding patterns of five wild Vigna species were studied. Sequential combined PI (propidium iodide) and DAPI (4',6-diamidino-2-phenylindole) (CPD) staining and fluorescence in situ hybridization (FISH) with 5S and 45S rDNA probes were used to analyze the karyotypes
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Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907. Comp. Cytogenet. (IF 1.036) Pub Date : 2020-05-27 Caio Augusto Gomes Goes,Sandro Natal Daniel,Lucas Henrique Piva,George Shigueki Yasui,Roberto Ferreira Artoni,Diogo Teruo Hashimoto,Fausto Foresti,Fábio Porto-Foresti
Astyanax Baird et Girard, 1854, is one of the largest genera in the family Characidae and comprises 177 valid species. This genus has been the focus of cytogenetic studies primarily owing to the presence of B chromosomes and high karyotypic diversity among different populations. The intense genetic variability in Astyanax is one of the factors responsible for the occurrence of species complexes, which
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Induction and evaluation of colchitetraploids of two species of Tinospora Miers, 1851. Comp. Cytogenet. (IF 1.036) Pub Date : 2020-05-20 Rakesh Kr Thakur,Vijay Rani Rajpal,Satyawada Rama Rao,Apekshita Singh,Lata Joshi,Pankaj Kaushal,Soom Nath Raina
Autotetraploidy, both natural and/or induced, has potential for genetic improvement of various crop species including that of medicinal importance. Tinospora cordifolia (Willdenow, 1806) Miers, 1851 ex Hooker et Thomson, 1855 and T. sinensis (Loureiro, 1790) Merrill, 1934 are two diploid species, which are dioecious, deciduous and climbing shrubs with high medicinal importance. Among the three methods
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Archives: The law of homologous series in variation (N. I. Vavilov). Comp. Cytogenet. (IF 1.036) Pub Date : 2020-07-14 Nina Bulatova
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Karyotype and putative chromosomal inversion suggested by integration of cytogenetic and molecular data of the fungus-farming ant Mycetomoellerius iheringi Emery, 1888. Comp. Cytogenet. (IF 1.036) Pub Date : 2020-05-07 Ricardo Micolino,Maykon Passos Cristiano,Danon Clemes Cardoso
Comparative cytogenetic analyses are being increasingly used to collect information on species evolution, for example, diversification of closely related lineages and identification of morphologically indistinguishable species or lineages. Here, we have described the karyotype of the fungus-farming ant Mycetomoellerius iheringi Emery, 1888 and investigated its evolutionary relationships on the basis
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Cloning and preliminary verification of telomere-associated sequences in upland cotton. Comp. Cytogenet. (IF 1.036) Pub Date : 2020-04-06 Yuling Liu,Zhen Liu,Yangyang Wei,Yanjun Wang,Jiaran Shuang,Renhai Peng
Telomeres are structures enriched in repetitive sequences at the end of chromosomes. In this study, using the telomere primer AA(CCCTAAA)3CCC for the single primer PCR, two DNA sequences were obtained from Gossypium hirsutum (Linnaeus, 1753) accession (acc.) TM-1. Sequence analysis showed that the two obtained sequences were all rich in A/T base, which was consistent with the characteristic of the
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Patterns of chromosomal variation in Mexican species of Aeschynomene (Fabaceae, Papilionoideae) and their evolutionary and taxonomic implications. Comp. Cytogenet. (IF 1.036) Pub Date : 2020-03-11 Fernando Tapia-Pastrana,Alfonso Delgado-Salinas,Javier Caballero
A cytogenetic analysis of sixteen taxa of the genus Aeschynomene Linnaeus, 1753, which includes species belonging to both subgenera Aeschynomene (Léonard, 1954) and Ochopodium (Vogel, 1838) J. Léonard, 1954, was performed. All studied species had the same chromosome number (2n = 20) but exhibited karyotype diversity originating in different combinations of metacentric, submetacentric and subtelocentric
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New data on karyotype, spermatogenesis and ovarian trophocyte ploidy in three aquatic bug species of the families Naucoridae, Notonectidae, and Belostomatidae (Nepomorpha, Heteroptera) Comp. Cytogenet. (IF 1.036) Pub Date : 2020-03-05 Desislava Stoianova; Nikolay Simov; Manh Quang Vu; Duc Minh Nguyen; Snejana Grozeva
We report the karyotype, some aspects of spermatogenesis, and ovarian trophocytes ploidy in three aquatic bug species: Ilyocoris cimicoides (Linnaeus, 1758), Notonecta glauca Linnaeus, 1758, and Diplonychus rusticus Fabricius, 1871 from previously unexplored regions – South Europe (Bulgaria) and Southeast Asia (Vietnam). Our results add considerable support for the published karyotype data for these
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Cytogenetics of entelegyne spiders (Arachnida, Araneae) from southern Africa Comp. Cytogenet. (IF 1.036) Pub Date : 2020-03-04 František Šťáhlavský; Martin Forman; Pavel Just; Filip Denič; Charles R. Haddad; Vera Opatova
Spiders represent one of the most studied arachnid orders. They are particularly intriguing from a cytogenetic point of view, due to their complex and dynamic sex chromosome determination systems. Despite intensive research on this group, cytogenetic data from African spiders are still mostly lacking. In this study, we describe the karyotypes of 38 species of spiders belonging to 16 entelegyne families
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Relationship between meiotic behaviour and fertility in backcross-1 derivatives of the [(Gossypium hirsutum × G. thurberi)2 × G. longicalyx] trispecies hybrid. Comp. Cytogenet. (IF 1.036) Pub Date : 2020-01-28 N'guessan Olivier Konan,Guy Mergeai
Wild cotton species are an important source of desirable genes for genetic improvement of cultivated cotton Gossypium hirsutum Linnaeus, 1763. For the success of such an improvement, chromosome pairings and recombinations in hybrids are fundamental. The wild African species G. longicalyx Hutchinson & Lee, 1958 could be used as donor of the desirable trait of fiber fineness. Twelve BC1 plants obtained
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Comparative cytogenetics of the ground frogs Eupsophus emiliopugini Formas, 1989 and E. vertebralis Grandison, 1961 (Alsodidae) with comments on their inter- and intraspecific chromosome differentiation. Comp. Cytogenet. (IF 1.036) Pub Date : 2020-01-27 Camila A Quercia,Elkin Y Suárez-Villota,Fausto Foresti,José J Nuñez
South American frogs of the genus Eupsophus Fitzinger, 1843 comprise 10 species. Two of them, Eupsophus vertebralis Grandison, 1961 and E. emiliopugini Formas, 1989 belong to the Eupsophus vertebralis group, exhibiting 2n = 28. Fundamental number differences between these species have been described using conventional chromosome staining of few specimens from only two localities. Here, classical techniques
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Cytogenetic data for sixteen ant species from North-eastern Amazonia with phylogenetic insights into three subfamilies. Comp. Cytogenet. (IF 1.036) Pub Date : 2020-01-22 Hilton Jeferson Alves Cardoso de Aguiar,Luísa Antônia Campos Barros,Linda Inês Silveira,Frédéric Petitclerc,Sandrine Etienne,Jérôme Orivel
Ants play essential roles in most terrestrial ecosystems and may be considered pests for agriculture and agroforestry. Recent morphological and molecular data have challenged conventional ant phylogeny and the interpretation of karyotypic variations. Existing Neotropical ant cytogenetic data focus on Atlantic rainforest species, and provide evolutionary and taxonomic insight. However, there are data
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Interspecific cytogenetic relationships in three Acestrohynchus species (Acestrohynchinae, Characiformes) reveal the existence of possible cryptic species. Comp. Cytogenet. (IF 1.036) Pub Date : 2020-01-15 Alber Sousa Campos,Ramon Marin Favarato,Eliana Feldberg
The karyotypes and chromosomal characteristics of three Acestrorhynchus Eigenmann et Kennedy, 1903 species were examined using conventional and molecular protocols. These species had invariably a diploid chromosome number 2n = 50. Acestrorhynchus falcatus (Block, 1794) and Acestrorhynchus falcirostris (Cuvier, 1819) had the karyotype composed of 16 metacentric (m) + 28 submetacentric (sm) + 6 subtelocentric
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Cytogenetic and pollen identification of genus Gagnepainia (Zingiberaceae) in Thailand. Comp. Cytogenet. (IF 1.036) Pub Date : 2020-01-13 Paramet Moonkaew,Nattapon Nopporncharoenkul,Thaya Jenjittikul,Puangpaka Umpunjun
Gagnepainia godefroyi K. Schumann, 1904 and G. harmandii K. Schumann, 1904 belong to the genus Gagnepainia K. Schumann, 1904 of the Ginger family. They have the potential to be developed as medicinal and attractive ornamental plants. To date, the knowledge on the cytological and reproductive aspects of Gagnepainia have not been publicly available. Therefore, the aims of this research are to investigate
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Triploidy in Chinese parthenogenetic Helophorus orientalis Motschulsky, 1860, further data on parthenogenetic H. brevipalpis Bedel, 1881 and a brief discussion of parthenogenesis in Hydrophiloidea (Coleoptera). Comp. Cytogenet. (IF 1.036) Pub Date : 2020-01-13 Robert B Angus,Fenglong Jia
The chromosomes of triploid parthenogenetic Helophorus orientalis Motschulsky, 1860 are described from material from two localities in Heilongjiang, China. 3n = 33. All the chromosomes have clear centromeric C-bands, and in the longest chromosome one replicate appears to be consistently longer than the other two. The chromosomes of additional triploid parthenogenetic H. brevipalpis Bedel, 1881, from
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Molecular-cytogenetic analysis of diploid wheatgrass Thinopyrum bessarabicum (Savul. and Rayss) A. Löve. Comp. Cytogenet. (IF 1.036) Pub Date : 2019-12-03 Ekaterina D Badaeva,Sergei A Surzhikov,Alexander V Agafonov
Thinopyrum bessarabicum (T. Săvulescu & T. Rayss, 1923) A. Löve, 1980 is diploid (2n=2x=14, JJ or EbEb), perennial self-fertilizing rhizomatous maritime beach grass, which is phylogenetically close to another diploid wheatgrass species, Agropyron elongatum (N. Host, 1797) P. de Beauvois, 1812. The detailed karyotype of Th. bessarabicum was constructed based on FISH with six DNA probes representing
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Description of the karyotype of Sphyracephala detrahens (Diptera, Diopsidae). Comp. Cytogenet. (IF 1.036) Pub Date : 2019-12-03 Ayumi Kudo
The eye stalks in Diopsidae (Bilberg, 1820) have been widely examined, but the evolutionary origin of this unique trait remains unclear. Thus, further studies of Sphiracephala (Say, 1828), the extant genus forming a basal branch of Diopsinae, are needed. The present study aimed to identify the karyotype of Sphyracephala detrahens (Walker, 1860) with conventional Giemsa staining. Cytogenetic analysis
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Karyotype of Polyommatus (Agrodiaetus) eriwanensis Forster, 1960 and taxonomic position of P. (A.) interjectus de Lesse, 1960 (Lepidoptera, Lycaenidae). Comp. Cytogenet. (IF 1.036) Pub Date : 2019-11-26 Vladimir A Lukhtanov,Alexander V Dantchenko
The karyotype of Polyommatus (Agrodiaetus) eriwanensis Forster, 1960 from the type locality ("Eriwan" [Yerevan, Armenia]) and other localities in Armenia was investigated. The number of chromosomal elements (bivalents+ multivalents) observed in male meiosis I was found to vary from 29 to 34. In individuals with n = 34, all observed elements were represented by bivalents. In other specimens, heterozygosity
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Karyotype characteristics, chromosomal polymorphism and gene COI sequences of Chironomus heteropilicornis Wülker, 1996 (Diptera, Chironomidae) from the South Caucasus. Comp. Cytogenet. (IF 1.036) Pub Date : 2019-11-15 Mukhamed Kh Karmokov
The study presents data on the karyotype characteristics, features of chromosomal polymorphism and the gene COI sequences of Chironomus heteropilicornis Wülker, 1996 (Diptera, Chironomidae) from the South Caucasus. We found 8 banding sequences in the Caucasian population. Overall, The Caucasian population of the species can be characterized as having a low level of polymorphism. We found one new banding
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Recurrent variation in the active NOR sites in the monkey frogs of the genus Pithecopus Cope, 1866 (Phyllomedusidae, Anura). Comp. Cytogenet. (IF 1.036) Pub Date : 2019-11-05 Joana Moura Gama,Camilla Borges Gazolla,Deborah Yasmin de Souza,Shirlei Maria Recco-Pimentel,Daniel Pacheco Bruschi
Treefrogs of the genus Pithecopus Cope, 1866 exhibit expressive chromosomal homogeneity which contrasts with a high variation frequency of the nucleolus organizer region (NOR) related to the group. Currently, the genus contains eleven species and no chromosomal data are available on P. palliatus Peters, 1873, P. ayeaye Lutz, 1966 and P. megacephalus Miranda-Ribeiro, 1926. Here, we describe the karyotypes
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Karyotype reinvestigation does not confirm the presence of two cryptic species and interspecific hybridization in the Polyommatus (Agrodiaetus) damocles complex in the Crimea (Lepidoptera, Lycaenidae). Comp. Cytogenet. (IF 1.036) Pub Date : 2019-10-31 Vladimir A Lukhtanov,Konstantin A Efetov,Alexander V Dantchenko
The karyotype of the blue butterflies from the Angarskiy Pass (Crimea), previously attributed to Polyommatus (Agrodiaetus) poseidon (Herrich-Schäffer, 1851), was re-examined. In all 19 studied individuals, we found the haploid chromosome number n = 26, including 7 pairs of relatively large and 19 pairs of relatively small chromosomes. According to the chromosome number and karyotype structure, the
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Chromosome spreading of the (TTAGGG)n repeats in the Pipa carvalhoi Miranda-Ribeiro, 1937 (Pipidae, Anura) karyotype. Comp. Cytogenet. (IF 1.036) Pub Date : 2019-10-28 Michelle Louise Zattera,Luana Lima,Iraine Duarte,Deborah Yasmin de Sousa,Olívia Gabriela Dos Santos Araújo,Thiago Gazoni,Tamí Mott,Shirlei Maria Recco-Pimentel,Daniel Pacheco Bruschi
Pipidae is a clade of Anura that diverged relatively early from other frogs in the phylogeny of the group. Pipids have a unique combination of morphological features, some of which appear to represent a mix of adaptations to aquatic life and plesiomorphic characters of Anura. The present study describes the karyotype of Pipa carvalhoi Miranda-Ribeiro, 1937, including morphology, heterochromatin distribution
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New evidence for the presence of the telomere motif (TTAGG) n in the family Reduviidae and its absence in the families Nabidae and Miridae (Hemiptera, Cimicomorpha). Comp. Cytogenet. (IF 1.036) Pub Date : 2019-10-04 Snejana Grozeva,Boris A Anokhin,Nikolay Simov,Valentina G Kuznetsova
Male karyotype and meiosis in four true bug species belonging to the families Reduviidae, Nabidae, and Miridae (Cimicomorpha) were studied for the first time using Giemsa staining and FISH with 18S ribosomal DNA and telomeric (TTAGG)n probes. We found that Rhynocoris punctiventris (Herrich-Schäffer, 1846) and R. iracundus (Poda, 1761) (Reduviidae: Harpactorinae) had 2n = 28 (24 + X1X2X3Y), whereas
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A cytogenetic analysis in two species of Cassidinae (Coleoptera, Chrysomelidae). Comp. Cytogenet. (IF 1.036) Pub Date : 2019-10-04 Eduard Petitpierre
Two species of Cassidinae have been chromosomally analyzed, Cassida humeralis Kraatz, 1874 from France, with 2n = 18, 8 + Xyp meioformula and Anacassis fuscata (Klug, 1829) from Uruguay, with 2n = 30, 14 + Xy meioformula. The karyotype of the former is composed of similar meta/submetacentric autosomes, a small X-chromosome and a tiny y-chromosome, as many other Cassida and tribe Cassidini species,
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Banded karyotype of Nelore cattle (Bos taurus indicus Linnaeus, 1758). Comp. Cytogenet. (IF 1.036) Pub Date : 2019-09-29 Andréia Pires Amancio,Sabrina Sara Moreira Duarte,Rafael Carneiro Silva,Alex Silva da Cruz,Danilo Conrado Silva,Claudio Carlos da Silva,Aparecido Divino da Cruz
Chromosome banding techniques were applied and standardized to obtain karyotype characteristics for the first time in Brazil of Nelore cattle - Bos taurus indicus Linnaeus, 1758 - (bovine subspecies most prominent in Brazilian livestock). Blood samples were collected from the animals of the School of Agrarian and Biological Sciences of the Pontifical Catholic University of Goiás, two males and two
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Karyotype structure and NOR activity in Brazilian Smilax Linnaeus, 1753 species (Smilacaceae). Comp. Cytogenet. (IF 1.036) Pub Date : 2019-09-19 Daniel Pizzaia,Vanessa M Oliveira-Maekawa,Aline R Martins,Mateus Mondin,Margarida L R Aguiar-Perecin
The genus Smilax Linnaeus, 1753 (Smilacaceae) is a large genus of dioecious plants distributed in tropical, subtropical and temperate regions. Some Smilax species have medicinal importance and their identification is important for the control of raw material used in the manufacture of phytotherapeutical products. The karyotypes of seven Brazilian Smilax species were investigated. Mitotic metaphases
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Development of new cytogenetic markers for Thinopyrum ponticum (Podp.) Z.-W. Liu & R.-C. Wang. Comp. Cytogenet. (IF 1.036) Pub Date : 2019-08-24 Pavel Yu Kroupin,Victoria M Kuznetsova,Ekaterina A Nikitina,Yury Ts Martirosyan,Gennady I Karlov,Mikhail G Divashuk
Thinopyrum ponticum (Podpěra, 1902) Z.-W. Liu & R.-C.Wang, 1993 is an important polyploid wild perennial Triticeae species that is widely used as a source of valuable genes for wheat but its genomic constitution has long been debated. For its chromosome identification, only a limited set of FISH probes has been used. The development of new cytogenetic markers for Th. ponticum chromosomes is of great
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Molecular cytogenetic characterization and phylogenetic analysis of four Miscanthus species (Poaceae). Comp. Cytogenet. (IF 1.036) Pub Date : 2019-08-21 Yan-Mei Tang,Liang Xiao,Yasir Iqbal,Jian-Feng Liao,Long-Qian Xiao,Zi-Li Yi,Chao-Wen She
Chromosomes of four Miscanthus (Andersson, 1855) species including M. sinensis (Andersson, 1855), M. floridulus (Schumann & Lauterb, 1901), M. sacchariflorus (Hackel, 1882) and M. lutarioriparius (Chen & Renvoize, 2005) were analyzed using sequentially combined PI and DAPI (CPD) staining and fluorescence in situ hybridization (FISH) with 45S rDNA probe. To elucidate the phylogenetic relationship among
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Dysfunction telomeres in embryonic fibroblasts and cultured in vitro pluripotent stem cells of Rattus norvegicus (Rodentia, Muridae). Comp. Cytogenet. (IF 1.036) Pub Date : 2019-08-14 Natalya S Zhdanova,Evgenia A Vaskova,Tatyana V Karamysheva,Julia M Minina,Nykolay B Rubtsov,Suren M Zakian
We studied the level of spontaneous telomere dysfunction in Rattus norvegicus (Berkenhout, 1769) (Rodentia, Muridae) embryonic fibroblasts (rEFs) and in cultured in vitro rat pluripotent stem cells (rPSCs), embryonic stem cells (rESCs) and induced pluripotent stem cells (riPSCs), on early passages and after prolonged cultivation. Among studied cell lines, rESCs showed the lowest level of telomere dysfunction
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Different behaviour of C-banded peri-centromeric heterochromatin between sex chromosomes and autosomes in Polyphagan beetles. Comp. Cytogenet. (IF 1.036) Pub Date : 2019-07-23 Anne-Marie Dutrillaux,Bernard Dutrillaux
Heterochromatin variation was studied after C-banding of male karyotypes with a XY sex formula from 224 species belonging to most of the main families of Coleoptera. The karyotypes were classified in relation with the ratio heterochromatin/euchromatin total amounts and the amounts of heterochromatin on autosomes and gonosomes were compared. The C-banded karyotypes of 19 species, representing characteristic
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Rapid chromosomal evolution in enigmatic mammal with XX in both sexes, the Alay mole vole Ellobiusalaicus Vorontsov et al., 1969 (Mammalia, Rodentia). Comp. Cytogenet. (IF 1.036) Pub Date : 2019-07-06 Irina Bakloushinskaya,Elena A Lyapunova,Abdusattor S Saidov,Svetlana A Romanenko,Patricia C M O'Brien,Natalia A Serdyukova,Malcolm A Ferguson-Smith,Sergey Matveevsky,Alexey S Bogdanov
Evolutionary history and taxonomic position for cryptic species may be clarified by using molecular and cytogenetic methods. The subterranean rodent, the Alay mole vole Ellobiusalaicus Vorontsov et al., 1969 is one of three sibling species constituting the subgenus Ellobius Fischer, 1814, all of which lost the Y chromosome and obtained isomorphic XX sex chromosomes in both males and females. E.alaicus
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Molecular and cytogenetic differentiation within the Lariophagusdistinguendus (Förster, 1841) species complex (Hymenoptera, Pteromalidae). Comp. Cytogenet. (IF 1.036) Pub Date : 2019-06-27 Christian König,Sina Paschke,Marie Pollmann,Ronja Reinisch,Cornelia Gantert,Justus Weber,Lars Krogmann,Johannes L M Steidle,Vladimir E Gokhman
Several strains of the apparently well-known cosmopolitan synanthropic parasitoid of coleopteran stored-product pests, Lariophagusdistinguendus (Förster, 1841) from Western Europe, were studied using DNA sequencing and chromosomal analysis. The presence of at least two cryptic species with different COI sequences and chromosome numbers (n = 5 and 6) was supported. The species with n = 6 is associated
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Identification of sex chromosomes in Eremiasvelox (Lacertidae, Reptilia) using lampbrush chromosome analysis. Comp. Cytogenet. (IF 1.036) Pub Date : 2019-06-01 Artem P Lisachov,Svetlana A Galkina,Alsu F Saifitdinova,Svetlana A Romanenko,Daria A Andreyushkova,Vladimir A Trifonov,Pavel M Borodin
Reptiles are good objects for studying the evolution of sex determination, since they have different sex determination systems in different lineages. Lacertid lizards have been long-known for possessing ZZ/ZW type sex chromosomes. However, due to morphological uniformity of lacertid chromosomes, the Z chromosome has been only putatively cytologically identified. We used lampbrush chromosome (LBC) analysis
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Karyotypes of three species of Hyperophora Brunner von Wattenwyl, 1878 (Tettigoniidae, Phaneropterinae) enable morphologically similar species to be distinguished. Comp. Cytogenet. (IF 1.036) Pub Date : 2019-05-03 Bruno Cansanção Silva,Lucas Henrique Bonfim Souza,Juliana Chamorro-Rengifo,Douglas Araujo
Phaneropterinae is the largest subfamily of Tettigoniidae, distributed across the globe. There are few cytogenetic studies regarding this group, as in the case of the genus group Aniarae, which represents only two karyotyped species. The current study aims to analyze cytogenetically three species of Hyperophora Brunner von Wattenwyl, 1878 from Brazil. The male diploid number of Hyperophoraminor Brunner
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Comparative cytogenetics among Leporinusfriderici and Leporellusvittatus populations (Characiformes, Anostomidae): focus on repetitive DNA elements. Comp. Cytogenet. (IF 1.036) Pub Date : 2019-05-02 Thais Aparecida Dulz,Carla Andrea Lorscheider,Viviane Demetrio Nascimento,Rafael Bueno Noleto,Orlando Moreira-Filho,Viviane Nogaroto,Marcelo Ricardo Vicari
Anostomidae are a neotropical fish family rich in number of species. Cytogenetically, they show a conserved karyotype with 2n = 54 chromosomes, although they present intraspecific/interspecific variations in the number and chromosomal location of repetitive DNA sequences. The aim of the present study was to perform a comparative description of the karyotypes of two populations of Leporinusfriderici
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Analysis of chromosome karyotype and genome size in echiuran Urechisunicinctus Drasche, 1880 (Polychaeta, Urechidae). Comp. Cytogenet. (IF 1.036) Pub Date : 2019-03-29 Zhenkui Qin,Xueyu Li,Danwen Liu,Qing Wang,Li Lu,Zhifeng Zhang
Karyotype and genome size are two primary cytogenetic characteristics of species, which are of great significance to the study of cytogenetics, taxonomy, phylogenesis, evolution as well as molecular biology. However, this basic cytogenetic information in echiurans is lacking. Therefore, we analyzed characteristics of karyotype and genome size in the echiuran worm Urechisunicinctus Drasche, 1880. In