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NF‐Y is critical for the proper growth of zebrafish embryonic heart and its cardiomyocyte proliferation genesis (IF 1.76) Pub Date : 2021-01-08 Jing Chen
The ubiquitous NF‐Y gene regulates the expression of different genes in various signaling pathways. However, the function of NF‐Y in zebrafish heart development is largely unknown. Previously we identified a same group of cell cycle related gene cluster (CCRG) was downregulated in the embryonic hearts with impeded growth due to various stresses. The promoter regions of these CCRG genes shared a most
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Xenopus epidermal and endodermal epithelia as models for mucociliary epithelial evolution, disease, and metaplasia genesis (IF 1.76) Pub Date : 2021-01-05 Peter Walentek
The Xenopus embryonic epidermis is a powerful model to study mucociliary biology, development, and disease. Particularly, the Xenopus system is being used to elucidate signaling pathways, transcription factor functions, and morphogenetic mechanisms regulating cell fate specification, differentiation and cell function. Thereby, Xenopus research has provided significant insights into potential underlying
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The power of zebrafish models for understanding the co‐occurrence of craniofacial and limb disorders genesis (IF 1.76) Pub Date : 2021-01-04 Brittany T. Truong; Kristin B. Artinger
Craniofacial and limb defects are two of the most common congenital anomalies in the general population. Interestingly, these defects are not mutually exclusive. Many patients with craniofacial phenotypes, such as orofacial clefting and craniosynostosis, also present with limb defects, including polydactyly, syndactyly, brachydactyly, or ectrodactyly. The gene regulatory networks governing craniofacial
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Xenopus leads the way: Frogs as a pioneering model to understand the human brain genesis (IF 1.76) Pub Date : 2020-12-27 Cameron R. T. Exner; Helen Rankin Willsey
From its long history in the field of embryology to its recent advances in genetics, Xenopus has been an indispensable model for understanding the human brain. Foundational studies that gave us our first insights into major embryonic patterning events serve as a crucial backdrop for newer avenues of investigation into organogenesis and organ function. The vast array of tools available in Xenopus laevis
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Using Xenopus to analyze neurocristopathies like Kabuki syndrome genesis (IF 1.76) Pub Date : 2020-12-22 Janina Schwenty‐Lara; Silke Pauli; Annette Borchers
Neurocristopathies are human congenital syndromes that arise from defects in neural crest (NC) development and are typically associated with malformations of the craniofacial skeleton. Genetic analyses have been very successful in identifying pathogenic mutations, however, model organisms are required to characterize how these mutations affect embryonic development thereby leading to complex clinical
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Reduction of histamine and enhanced spinning behavior of Daphnia magna caused by scarlet mutant genesis (IF 1.76) Pub Date : 2020-12-21 Nur Izzatur Binti Ismail; Yasuhiko Kato; Tomoaki Matsuura; Cristian Gómez‐Canela; Carlos Barata; Hajime Watanabe
The ABC transporter, Scarlet, and its binding partner, White are involved in pigment synthesis in the insect eye and mutations in these genes are used as genetic markers. Recent studies have suggested that these transporters also have additional functions in the neuronal system. In our previous study, we generated scarlet mutant in the small crustacean, Daphnia magna and showed that the mutant lacked
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The plastic genome: The impact of transposable elements on gene functionality and genomic structural variations genesis (IF 1.76) Pub Date : 2020-11-24 Marco Fambrini; Gabriele Usai; Alberto Vangelisti; Flavia Mascagni; Claudio Pugliesi
Transposable elements (TEs) are DNA sequences that can change their position within genomes. TEs are present in most organisms and can be an important genomic component. Their activities are manifold: restructuring of genome size, chromosomal rearrangements, induction of gene mutations, and alteration of gene activity by insertion near or within promoters, intronic regions, or enhancer. There are several
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Caloric restriction upregulates the expression of DNMT3.1, lacking the conserved catalytic domain, in Daphnia magna genesis (IF 1.76) Pub Date : 2020-11-18 Nhan Duc Nguyen; Tomoaki Matsuura; Yasuhiko Kato; Hajime Watanabe
DNA methylation plays an important role in many aspects of biology, including development, disease, and phenotypic plasticity. In the branchiopod crustacean, Daphnia, de novo DNA methylation has been detected in specific environmental contexts. However, fundamental information on de novo DNA methyltransferase DNMT3 orthologs, including domain organization, developmental expression, and response to
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Activation of WNT signaling restores the facial deficits in a zebrafish with defects in cholesterol metabolism genesis (IF 1.76) Pub Date : 2020-11-16 Victoria L. Castro; Nayeli G. Reyes‐Nava; Brianna B. Sanchez; Cesar G. Gonzalez; David Paz; Anita M. Quintana
Inborn errors of cholesterol metabolism occur as a result of mutations in the cholesterol synthesis pathway (CSP). Although mutations in the CSP cause a multiple congenital anomaly syndrome, craniofacial abnormalities are a hallmark phenotype associated with these disorders. Previous studies have established that mutation of the zebrafish hmgcs1 gene (Vu57 allele), which encodes the first enzyme in
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Ray flower initiation in the Helianthus radula inflorescence is influenced by a functional allele of the HrCYC2c gene genesis (IF 1.76) Pub Date : 2020-12-01 Marco Fambrini; Rodolfo Bernardi; Claudio Pugliesi
The radiate pseudanthium, with actinomorphic disk flowers surrounded by showy marginal zygomorphic ray flowers, is the most common inflorescence in the Helianthus genus. In Helianthus radula, ray flower primordia are normally absent at the dorsal domain of the inner phyllaries (discoid heads) while the occurrence of radiate inflorescences is uncommon. In Helianthus spp., flower symmetry and inflorescence
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An E3 ubiquitin ligase, cullin‐4 regulates retinal differentiation in Drosophila eye genesis (IF 1.76) Pub Date : 2020-09-29 Meghana Tare; Anuradha Venkatakrishnan Chimata; Neha Gogia; Sonia Narwal; Prajakta Deshpande; Amit Singh
During organogenesis, cell proliferation is followed by the differentiation of specific cell types to form an organ. Any aberration in differentiation can result in developmental defects, which can result in a partial to a near‐complete loss of an organ. We employ the Drosophila eye model to understand the genetic and molecular mechanisms involved in the process of differentiation. In a forward genetic
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Using an aquatic model, Xenopus laevis, to uncover the role of chromodomain 1 in craniofacial disorders. genesis (IF 1.76) Pub Date : 2020-09-11 Brent H Wyatt,Thomas O Raymond,Lisa A Lansdon,Benjamin W Darbro,Jeffrey C Murray,John Robert Manak,Amanda J G Dickinson
The chromodomain family member chromodomain 1 (CHD1) has been shown to have numerous critical molecular functions including transcriptional regulation, splicing, and DNA repair. Complete loss of function of this gene is not compatible with life. On the other hand, missense and copy number variants of CHD1 can result in intellectual disabilities and craniofacial malformations in human patients including
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Cover Image, Volume 58, Issue 8 genesis (IF 1.76) Pub Date : 2020-08-26 Sapna Syal, Chris Ng, Yunah Kim, Javier Janbieh, Shubha Govind, Girish Deshpande
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The following two articles for this Special Issue were previously published and can be found in their respective issues online. genesis (IF 1.76) Pub Date : 2020-08-26
Reactive oxygen species signaling in primordial germ cell development in Drosophila embryos (58:6)
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Intron with transgenic marker (InTraM) facilitates high-throughput screening of endogenous gene reporter lines. genesis (IF 1.76) Pub Date : 2020-08-11 Russell T Collins,Baptiste Coxam,Ines Fechner,Iris Unterweger,Anna Szymborska,Katja Meier,Holger Gerhardt
The generation and maintenance of genome edited zebrafish lines is typically labor intensive due to the lack of an easy visual read‐out for the modification. To facilitate this process, we have developed a novel method that relies on the inclusion of an artificial intron with a transgenic marker (InTraM) within the knock‐in sequence of interest, which upon splicing produces a transcript with a precise
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Primordial germ cell-specific expression of eGFP in transgenic chickens. genesis (IF 1.76) Pub Date : 2020-08-09 Yota Hagihara,Yuya Okuzaki,Kazuma Matsubayashi,Hidenori Kaneoka,Takayuki Suzuki,Shinji Iijima,Ken-Ichi Nishijima
PR domain zinc finger protein 14 (PRDM14) plays an essential role in the development of primordial germ cells (PGCs) in mice. However, its functions in avian species remain unclear. In the present study, we used CRISPR/Cas9 to edit the PRDM14 locus in chickens in order to demonstrate its importance in development. The eGFP gene was introduced into the PRDM14 locus of cultured chicken PGCs to knockout
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Exploring germline recombination in Nestin-Cre transgenic mice using floxed androgen receptor. genesis (IF 1.76) Pub Date : 2020-08-03 Victoria M McLeod,Brittany Cuic,Mathew D F Chiam,Chew L Lau,Bradley J Turner
The Cre‐loxP strategy for tissue selective gene deletion has become a widely employed tool in neuroscience research. The validity of these models is largely underpinned by the temporal and spatial selectivity of recombinase expression under the promoter of the Cre driver line. Ectopic Cre‐recombinase expression gives rise to off‐target effects which can confound results and is especially detrimental
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Cover Image, Volume 58, Issue 7 genesis (IF 1.76) Pub Date : 2020-07-13 Joshua Sanchez, Risa Miyake, Andrew Cheng, Ting Liu, Sachiko Iseki, Tsutomu Kume
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Generation of transgenic mice that conditionally express microRNA miR-145. genesis (IF 1.76) Pub Date : 2020-07-10 Dwitiya Sawant,Emilie Klevenow,Jeremy T Baeten,Shelby Thomas,Sathiyanarayanan Manivannan,Simon J Conway,Brenda Lilly
MicroRNAs are modulators of cellular phenotypes and their functions contribute to development, homeostasis, and disease. miR‐145 is a conserved microRNA that has been implicated in regulating an array of phenotypes. These include supporting smooth muscle differentiation, repression of stem cell pluripotency, and inhibition of tumor growth and metastasis. Previously, our lab demonstrated that miR‐145
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Diphtheria toxin-mediated transposon-driven poly (A)-trapping efficiently disrupts transcriptionally silent genes in embryonic stem cells. genesis (IF 1.76) Pub Date : 2020-07-09 Jie Bai,Ryohei Kondo,N Ika Mayasari,Toshiaki Shigeoka,Ayako Isotani,Masahito Ikawa,Goro Sashida,Masashi Kawaichi,Yasumasa Ishida
Random gene trapping is the application of insertional mutagenesis techniques that are conventionally used to inactivate protein‐coding genes in mouse embryonic stem (ES) cells. Transcriptionally silent genes are not effectively targeted by conventional random gene trapping techniques, thus we herein developed an unbiased poly (A) trap (UPATrap) method using a Tol2 transposon, which preferentially
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A novel Axin2 knock-in mouse model for visualization and lineage tracing of WNT/CTNNB1 responsive cells. genesis (IF 1.76) Pub Date : 2020-07-09 Anoeska Agatha Alida van de Moosdijk,Yorick Bernardus Cornelis van de Grift,Saskia Madelon Ada de Man,Amber Lisanne Zeeman,Renée van Amerongen
Wnt signal transduction controls tissue morphogenesis, maintenance and regeneration in all multicellular animals. In mammals, the WNT/CTNNB1 (Wnt/β‐catenin) pathway controls cell proliferation and cell fate decisions before and after birth. It plays a critical role at multiple stages of embryonic development, but also governs stem cell maintenance and homeostasis in adult tissues. However, it remains
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Generation of Pecam1 endothelial specific dual reporter mouse model. genesis (IF 1.76) Pub Date : 2020-07-03 Donghua Hu,Lu Zhang,Fan Yang,Fuxue Chen,Deqiang Li,Chen-Leng Cai
Endothelial cells are specialized epithelium lining the interior surface of vessels and play fundamental roles in angiogenesis, vascular permeability, and immune response. To identify endothelial cells in vivo, we constructed a Pecam1nlacZ‐H2B‐GFP/+ knock‐in mouse model in which the endothelial cells are labeled by nuclear LacZ (nlacZ) expression. When Pecam1nlacZ‐H2B‐GFP/+ mice are bred with germline
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A cell type annotation Jamboree-Revival of а communal science forum. genesis (IF 1.76) Pub Date : 2020-06-25 Leonid Peshkin,Marc W Kirschner
Cell Atlases are currently being constructed for human tissues as well as several model organisms. New technologies make creation of vast datasets in many species possible, but the value of such data crucially depends on the quality of annotation. The tools of annotating single cell data and creating knowledge representations comparable across organisms have been lagging. We argue that successfully
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Reporter mice for isolating and auditing cell type-specific extracellular vesicles in vivo. genesis (IF 1.76) Pub Date : 2020-06-16 James V McCann,Steven R Bischoff,Yu Zhang,Dale O Cowley,Veronica Sanchez-Gonzalez,George D Daaboul,Andrew C Dudley
Extracellular vesicles (EVs) are abundant, lipid‐enclosed vectors that contain nucleic acids and proteins, they can be secreted from donor cells and freely circulate, and they can be engulfed by recipient cells thus enabling systemic communication between heterotypic cell types. However, genetic tools for labeling, isolating, and auditing cell type‐specific EVs in vivo, without prior in vitro manipulation
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Development and characterization of an Otp conditional loss of function allele. genesis (IF 1.76) Pub Date : 2020-05-28 Yu Hu,Jiamin Li,Yuyuan Zhu,Mengqi Li,Jianbang Lin,Lixin Yang,Chuan Wang,Zhonghua Lu
Orthopedia (Otp) is a homeodomain transcription factor that plays an essential role in the development of hypothalamic neurosecretory systems. Loss of Otp results in the failure of differentiation of key hypothalamic neuroendocrine cell types, and pups die soon after birth. Although the constitutive knockout Otp mouse model (Otp KO) has significantly expanded our understanding of Otp's function in
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A reporter mouse for in vivo detection of DNA damage in embryonic germ cells. genesis (IF 1.76) Pub Date : 2020-04-28 Jordana C Bloom,John C Schimenti
Maintaining genome integrity in the germline is essential for survival and propagation of a species. In both mouse and human, germ cells originate during fetal development and are hypersensitive to both endogenous and exogenous DNA damaging agents. Currently, mechanistic understanding of how primordial germ cells respond to DNA damage is limited in part by the tools available to study these cells.
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A zebrafish hox gene acts before gastrulation to specify the hemangioblast. genesis (IF 1.76) Pub Date : 2020-04-17 Changqing Zhang,Mark Featherstone
Hox genes encode transcription factors that have been implicated in embryonic, adult and disease processes. The earliest developmental program known to be directed by Hox genes is the timing of ingression of presumptive axial mesoderm during gastrulation. We previously used morpholino (MO)‐based knockdown to implicate the zebrafish hoxd4a gene in the specification of the hemangioblast, an event occurring
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Reactive oxygen species signaling in primordial germ cell development in Drosophila embryos. genesis (IF 1.76) Pub Date : 2020-04-17 Sapna Syal,Chris Ng,Yunah Kim,Javier Janbieh,Shubha Govind,Girish Deshpande
REDOX mechanisms that induce biosynthesis of the reactive oxygen species (ROS) have attracted considerable attention due to both the deleterious and beneficial responses elicited by the reactive radical. In several organisms including Drosophila melanogaster, modulation of ROS activity is thought to be crucial for the maintenance of cell fates in developmental contexts. Interestingly, REDOX mechanisms
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Generation of B6-Ddx4em1(CreERT2)Utr , a novel CreERT2 knock-in line, for germ cell lineage by CRISPR/Cas9. genesis (IF 1.76) Pub Date : 2020-04-15 Hoai Thu Le,Yoshikazu Hasegawa,Yoko Daitoku,Kanako Kato,Saori Miznuo-Iijima,Tra Thi Huong Dinh,Yumeno Kuba,Yuki Osawa,Natsuki Mikami,Kento Morimoto,Shinya Ayabe,Yoko Tanimoto,Kazuya Murata,Ken-Ichi Yagami,Satoru Takahashi,Seiya Mizuno,Fumihiro Sugiyama
Germ cell development is essential for maintaining reproduction in animals. In postpubertal females, oogenesis is a highly complicated event for producing fertilizable oocytes. It starts when dormant primordial oocytes undergo activation to become growing oocytes. In postpubertal males, spermatogenesis is a differentiation process for producing sperm from spermatogonial stem cells. To obtain full understanding
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Simple embryo injection of long single-stranded donor templates with the CRISPR/Cas9 system leads to homology-directed repair in Xenopus tropicalis and Xenopus laevis. genesis (IF 1.76) Pub Date : 2020-04-11 Takuya Nakayama,Robert M Grainger,Sang-Wook Cha
We report model experiments in which simple microinjection of fertilized eggs has been used to effectively perform homology‐directed repair (HDR)‐mediated gene editing in the two Xenopus species used most frequently for research: X. tropicalis and X. laevis. We have used long single‐stranded DNAs having phosphorothioate modifications as donor templates for HDR at targeted genomic sites using the Clustered
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Generation and characterization of Six2 conditional mice. genesis (IF 1.76) Pub Date : 2020-04-10 Chaochang Li,Han Liu,Yueh-Chiang Hu,Yu Lan,Rulang Jiang
Heterozygous deletion of Six2, which encodes a member of sine oculis homeobox family transcription factors, has recently been associated with the frontonasal dysplasia syndrome FND4. Previous studies showed that Six2 is expressed in multiple tissues during craniofacial development in mice, including embryonic head mesoderm, postmigratory frontonasal neural crest cells, and epithelial and mesenchymal
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Conditional inactivation of Foxc1 and Foxc2 in neural crest cells leads to cardiac abnormalities. genesis (IF 1.76) Pub Date : 2020-04-07 Joshua Sanchez,Risa Miyake,Andrew Cheng,Ting Liu,Sachiko Iseki,Tsutomu Kume
Cardiac neural crest cells (cNCCs) are required for normal heart development. cNCCs are a multipotent and migratory cell lineage that differentiates into multiple cell types. cNCCs migrate into the developing heart to contribute to the septation of the cardiac outflow tract (OFT). Foxc1 and Foxc2 are closely related members of the FOX (Forkhead box) transcription factor family and are expressed in
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Cover Image, Volume 58, Issue 3‐4 genesis (IF 1.76) Pub Date : 2020-03-19 Junnan Fang, Dorothy A. Lerit
Cover illustration: The front cover image is based on the Original Article Drosophila Pericentrin‐like protein promotes the formation of primordial germ cells by Dorothy Lerit and Junnan Fang, https://doi.org/10.1002/dvg.23347.
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Cover Image, Volume 58, Issue 3‐4 genesis (IF 1.76) Pub Date : 2020-03-19 Qun Lin, Yan Huang, Frank J. Giordano, Zhong Yun
Cover illustration: The back cover image is based on the Short Communication Generation of a hypoxia‐sensing mouse model by Zhong Yun, Qun Lin, Frank, J. Giordano et al., https://doi.org/10.1002/dvg.23352.
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Generation of an Fsp1 (fibroblast-specific protein 1)-Flpo transgenic mouse strain. genesis (IF 1.76) Pub Date : 2020-03-19 Victoire Cardot-Ruffino,Véronique Chauvet,Cassandre Caligaris,Adrien Bertrand-Chapel,Nicolas Chuvin,Roxane M Pommier,Ulrich Valcourt,David Vincent,Sylvie Martel,Sophie Aires,Bastien Kaniewski,Pierre Dubus,Philippe Cassier,Stéphanie Sentis,Laurent Bartholin
Recombination systems represent a major breakthrough in the field of genetic model engineering. The Flp recombinases (Flp, Flpe, and Flpo) bind and cleave DNA Frt sites. We created a transgenic mouse strain ([Fsp1‐Flpo]) expressing the Flpo recombinase in fibroblasts. This strain was obtained by random insertion inside mouse zygotes after pronuclear injection. Flpo expression was placed under the control
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CRISPR/Cas9 engineering of albino cystinuria Type A mice. genesis (IF 1.76) Pub Date : 2020-02-20 Thomas M Beckermann,Richard C Welch,Felisha M Williams,Douglas P Mortlock,Feng Sha,Talat A Ikizler,Lauren E Woodard,Matthew H Wilson
Cystinuria Type A is a relatively common genetic kidney disease occurring in 1 in 7,000 people worldwide that results from mutation of the cystine transporter rBAT encoded by Slc3a1. We used CRISPR/Cas9 technology to engineer cystinuria Type A mice via genome editing of the C57BL/6NHsd background. These mice are an improvement on currently available models as they are on a coisogenic genetic background
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Neural crest and placode roles in formation and patterning of cranial sensory ganglia in lamprey. genesis (IF 1.76) Pub Date : 2020-02-12 Tian Yuan,Joshua R York,David W McCauley
Vertebrates possess paired cranial sensory ganglia derived from two embryonic cell populations, neural crest and placodes. Cranial sensory ganglia arose prior to the divergence of jawed and jawless vertebrates, but the developmental mechanisms that facilitated their evolution are unknown. Using gene expression and cell lineage tracing experiments in embryos of the sea lamprey, Petromyzon marinus, we
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A rat H1t-GFP transgene recapitulates endogenous H1t expression pattern in mouse. genesis (IF 1.76) Pub Date : 2020-01-28 Emma Lipschutz,Anindya Dasgupta,Yongjuan Guan,W Stephen Kistler,P Jeremy Wang
H1 histones bind to linker DNA. H1t (H1f6), a testis‐specific linker histone variant, is present in pachytene spermatocytes and spermatids. The expression of H1t histone coincides with the acquisition of metaphase I competence in pachytene spermatocytes. Here we report the generation of H1t‐GFP transgenic mice. The H1t‐GFP (H1 histone testis‐green fluorescence protein) fusion protein expression recapitulates
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Cover Image, Volume 58, Issue 1 genesis (IF 1.76) Pub Date : 2020-01-14 Taro Okunomiya, Hiroyuki Hioki, Chika Nishimura, Satoshi Yawata, Itaru Imayoshi, Ryoichiro Kageyama, Ryosuke Takahashi, Dai Watanabe
Cover illustration: The cover image is based on the Short Communication Generation of a MOR‐CreER knock‐in mouse line to study cells and neural circuits involved in mu opioid receptor signaling by Dai Watanabe et al., https://doi.org/10.1002/dvg.23341.
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miR‐199 plays both positive and negative regulatory roles in Xenopus eye development genesis (IF 1.76) Pub Date : 2020-01-07 Ruth A. Ritter, Christina H. Ulrich, Bogna N. Brzezinska, Vrutant V. Shah, Melissa J. Zamora, Lisa E. Kelly, Heithem M. El‐Hodiri, Amy K. Sater
To investigate microRNA (miR) functions in early eye development, we asked whether eye field transcription factors (EFTFs) are targets of miR‐dependent regulation in Xenopus embryos. Argonaute (AGO) ribonucleoprotein complexes, including miRs and targeted mRNAs, were coimmunoprecipitated from transgenic embryos expressing myc‐tagged AGO under the control of the rax1 promoter; mRNAs for all EFTFs coimmunoprecipitated
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Generation of a hypoxia‐sensing mouse model genesis (IF 1.76) Pub Date : 2019-12-24 Qun Lin, Yan Huang, Frank J. Giordano, Zhong Yun
Oxygen (O2) homeostasis is essential to the metazoan life. O2‐sensing or hypoxia‐regulated molecular pathways are intimately involved in a wide range of critical cellular functions and cell survival from embryogenesis to adulthood. In this report, we have designed an innovative hypoxia sensor (O2CreER) based on the O2‐dependent degradation domain of the hypoxia‐inducible factor‐1α and Cre recombinase
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Development and functional characterization of a lncRNA‐HIT conditional loss of function allele genesis (IF 1.76) Pub Date : 2019-12-14 Hanqian L. Carlson, H. Scott Stadler
Analysis of the human and murine transcriptomes has identified long noncoding RNAs (lncRNAs) as major functional components in both species. Transcriptional profiling of the murine limb led to our discovery of lncRNA‐HIT, which our previous in vitro analyses suggested a potential role for this lncRNA in the development of limb, craniofacial, and genitourinary tissues (Carlson et al., 2015). To test
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Sleep deprivation decreases the reproductive capacity by affecting the arrival of morulas in the uterus genesis (IF 1.76) Pub Date : 2019-12-09 Bruno Frederico Aguilar Calegare, Augusto Azzolini, Julia Ribeiro da Silva Vallim, Edson Guimarães Lo Turco, Priscila Farias Tempaku, Vanessa Cavalcante da Silva, Sergio Tufik, Vânia D'Almeida
A previous animal study by our group found that sleep deprivation during preimplantation was associated with decreased pregnancy maintenance. Given its impact on human society, we aimed in the current study to assess whether sleep deprivation affects blastocyst gene expression and/or the implantation process. For this, pregnant mice (gestational day 0 [GD 0]) were assigned into paradoxical sleep deprivation
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Cover Image, Volume 57, Issue 11‐12 genesis (IF 1.76) Pub Date : 2019-12-02 Maiko Terada, Masaki Kawamata, Ryota Kimura, Sayaka Sekiya, Go Nagamatsu, Katsuhiko Hayashi, Kenichi Horisawa, Atsushi Suzuki
Cover illustration: The cover image is based on the Technology Report Generation of Nanog reporter mice that distinguish pluripotent stem cells from unipotent primordial germ cells by Atsushi Suzuki et al., https://doi.org/10.1002/dvg.23334.
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Drosophila pericentrin-like protein promotes the formation of primordial germ cells. genesis (IF 1.76) Pub Date : 2019-11-27 Junnan Fang,Dorothy A Lerit
Primordial germ cells (PGCs) are the precursors to the adult germline stem cells that are set aside early during embryogenesis and specified through the inheritance of the germ plasm, which contains the mRNAs and proteins that function as the germline fate determinants. In Drosophila melanogaster, formation of the PGCs requires the microtubule and actin cytoskeletal networks to actively segregate the
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Developmental aspects of the tympanic membrane: Shedding light on function and disease. genesis (IF 1.76) Pub Date : 2019-11-25 Mona Mozaffari,Dan Jiang,Abigail S Tucker
The ear drum, or tympanic membrane (TM), is a key component in the intricate relay that transmits air‐borne sound to our fluid‐filled inner ear. Despite early belief that the mammalian ear drum evolved as a transformation of a reptilian drum, newer fossil data suggests a parallel and independent evolution of this structure in mammals. The term “drum” belies what is in fact a complex three‐dimensional
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Generation of PECAM-1 (CD31) conditional knockout mice. genesis (IF 1.76) Pub Date : 2019-11-15 Huiying Zhi,Taisuke Kanaji,Guoping Fu,Debra K Newman,Peter J Newman
Platelet endothelial cell adhesion molecule 1 (PECAM‐1) is an adhesion and signaling receptor that is expressed on endothelial and hematopoietic cells and plays important roles in angiogenesis, vascular permeability, and regulation of cellular responsiveness. To better understanding the tissue specificity of PECAM‐1 functions, we generated mice in which PECAM1, the gene encoding PECAM‐1, could be conditionally
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The role of chick Ebf genes in the mediolateral patterning of the somites. genesis (IF 1.76) Pub Date : 2019-11-13 Mohammed A El-Magd,Shafika A Elsayed,Eman S El-Shetry,Ahmed Abdelfattah-Hassan,Ayman A Saleh,Steve Allen,Imelda McGonnell,Ketan Patel
This study was conducted to check whether the three chick Early B‐cell Factor (Ebf) genes, particularly cEbf1, would be targets for Shh and Bmp signals during somites mediolateral (ML) patterning. Tissue manipulations and gain and loss of function experiments for Shh and Bmp4 were performed and the results revealed that cEbf1 expression was initiated in the cranial presomitic mesoderm by low dose of
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Serotonin and MucXS release by small secretory cells depend on Xpod, a SSC specific marker gene. genesis (IF 1.76) Pub Date : 2019-11-08 Yvonne Kurrle,Katharina Kunesch,Susanne Bogusch,Axel Schweickert
Mucus secretion and ciliary motility are hallmarks for muco‐ciliary epithelia (MCE). Both, mammalian airways as well as the less complex epidermis of Xenopus embryos show cilia‐driven mucus flow to protect the organism against harmful effects by exogenous pathogens or pollutants. Four cell types set up the epidermal MCE in Xenopus. Multi‐ciliated cells (MCCs) generate an anterior to posterior flow
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Mypt1 regulates Bmp signaling to promote embryonic exocrine pancreas growth in zebrafish. genesis (IF 1.76) Pub Date : 2019-11-08 Guoping Dong,Yueyue Huang,Huimei Ding,Lingfei Luo,Yaoguang Zhang,Honghui Huang,Hua Ruan
Myosin phosphatase targeting subunit 1 (Mypt1) is the regulatory subunit of myosin phosphatase which dephosphorylates the light chain of myosin II to inhibit its contraction. Although biochemical properties of Mypt1 have been characterized in detail, its biological functions in organisms are not well understood. The zebrafish mypt1 sq181 allele was found defective in the ventral pancreatic bud and
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Reduced maternal vitamin A status increases the incidence of normal aortic arch variants. genesis (IF 1.76) Pub Date : 2019-11-05 Kelsey F Sugrue,Irene E Zohn
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Identification of transcripts potentially involved in neural tube closure using RNA sequencing. genesis (IF 1.76) Pub Date : 2019-10-12 Lexy M Kindt,Alicia R Coughlin,Tianna R Perosino,Haley N Ersfeld,Marshall Hampton,Jennifer O Liang
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Preface: Celebrating 150 Years of Neural Crest Research. genesis (IF 1.76) Pub Date : 2018-08-23 Sophie Creuzet,Jean-Pierre Saint-Jeannet
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Genesis special issue: New models for arthropod research. genesis (IF 1.76) Pub Date : 2017-05-10 Helena Araujo
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Generation of a MOR-CreER knock-in mouse line to study cells and neural circuits involved in mu opioid receptor signaling. genesis (IF 1.76) Pub Date : 2019-10-25 Taro Okunomiya,Hiroyuki Hioki,Chika Nishimura,Satoshi Yawata,Itaru Imayoshi,Ryoichiro Kageyama,Ryosuke Takahashi,Dai Watanabe
Mu opioid receptor (MOR) is involved in various brain functions, such as pain modulation, reward processing, and addictive behaviors, and mediates the main pharmacologic effects of morphine and other opioid compounds. To gain genetic access to MOR‐expressing cells, and to study physiological and pathological roles of MOR signaling, we generated a MOR‐CreER knock‐in mouse line, in which the stop codon
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Creation of zebrafish knock-in reporter lines in the nefma gene by Cas9-mediated homologous recombination. genesis (IF 1.76) Pub Date : 2019-09-30 Alexis Eschstruth,Sylvie Schneider-Maunoury,François Giudicelli
CRISPR/Cas9‐based strategies are widely used for genome editing in many organisms, including zebrafish. Although most applications consist in introducing double strand break (DSB)‐induced mutations, it is also possible to use CRISPR/Cas9 to enhance homology directed repair (HDR) at a chosen genomic location to create knock‐ins with optimally controlled precision. Here, we describe the use of CRISPR/Cas9‐targeted
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Increased activity of mesenchymal ALK2-BMP signaling causes posteriorly truncated microglossia and disorganization of lingual tissues. genesis (IF 1.76) Pub Date : 2019-09-30 Mohamed Ishan,Guiqian Chen,Chenming Sun,Shi-You Chen,Yoshihiro Komatsu,Yuji Mishina,Hong-Xiang Liu
Proper development of taste organs including the tongue and taste papillae requires interactions with the underlying mesenchyme through multiple molecular signaling pathways. The effects of bone morphogenetic proteins (BMPs) and antagonists are profound, however, the tissue‐specific roles of distinct receptors are largely unknown. Here, we report that constitutive activation (ca) of ALK2‐BMP signaling
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