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Deficits in olfactory system neurogenesis in neurodevelopmental disorders genesis (IF 1.5) Pub Date : 2024-03-16 Sean C. Sweat, Claire E. J. Cheetham
SummaryThe role of neurogenesis in neurodevelopmental disorders (NDDs) merits much attention. The complex process by which stem cells produce daughter cells that in turn differentiate into neurons, migrate various distances, and form synaptic connections that are then refined by neuronal activity or experience is integral to the development of the nervous system. Given the continued postnatal neurogenesis
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Molecular mechanisms of differentiation and class choice of olfactory sensory neurons genesis (IF 1.5) Pub Date : 2024-03-08 Junji Hirota
SummaryThe sense of smell is intricately linked to essential animal behaviors necessary for individual survival and species preservation. During vertebrate evolution, odorant receptors (ORs), responsible for detecting odor molecules, have evolved to adapt to changing environments, transitioning from aquatic to terrestrial habitats and accommodating increasing complex chemical environments. These evolutionary
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Cover Image, Volume 62, Issue 1 genesis (IF 1.5) Pub Date : 2024-02-26 Mingyi Zhang, Jifan Feng, Yue Li, Peter Z. Qin, Yang Chai
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Activity-dependent formation of the topographic map and the critical period in the development of mammalian olfactory system genesis (IF 1.5) Pub Date : 2024-01-30 Ai Fang, C. Ron Yu
Neural activity influences every aspect of nervous system development. In olfactory systems, sensory neurons expressing the same odorant receptor project their axons to stereotypically positioned glomeruli, forming a spatial map of odorant receptors in the olfactory bulb. As individual odors activate unique combinations of glomeruli, this map forms the basis for encoding olfactory information. The
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The Prl3d1-Cre mouse line selectively induces the expression of Cre recombinase in parietal trophoblast giant cells genesis (IF 1.5) Pub Date : 2023-12-20 Linqing Pan, Fuquan Zhu, Aochen Yu, Yuan Jiang, Dayu Wang, Minglian Zhou, Chao Jia, Yugui Cui, Lisha Tang, Huaiyun Tang, Juan Li
The placenta plays a pivotal role in the maintenance of normal pregnancy, but how it forms, matures, and performs its function remains poorly understood. Here, we describe a novel mouse line (Prl3d1-iCre) that expresses iCre recombinase under the control of the endogenous prl3d1 promoter. Prl3d1 has been proposed as a marker for distinguishing trophoblast giant cells (TGCs) from other trophoblast cells
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Generation of a Dcx-CreERT2 knock-in mouse for genetic manipulation of newborn neurons genesis (IF 1.5) Pub Date : 2023-12-16 Gabriella A. Perez, Kyung-Won Park, Denise Lanza, Jenna Cicardo, M. Danish Uddin, Joanna L. Jankowsky
A wide variety of CreERT2 driver lines are available for genetic manipulation of adult-born neurons in the mouse brain. These tools have been instrumental in studying fate potential, migration, circuit integration, and morphology of the stem cells supporting lifelong neurogenesis. Despite a wealth of tools, genetic manipulation of adult-born neurons for circuit and behavioral studies has been limited
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Generation of tamoxifen-inducible Tfap2b-CreERT2 mice using CRISPR-Cas9 genesis (IF 1.5) Pub Date : 2023-12-08 Mingyi Zhang, Jifan Feng, Yue Li, Peter Z. Qin, Yang Chai
Tfap2b, a pivotal transcription factor, plays critical roles within neural crest cells and their derived lineage. To unravel the intricate lineage dynamics and contribution of these Tfap2b+ cells during craniofacial development, we established a Tfap2b-CreERT2 knock-in transgenic mouse line using the CRISPR-Cas9-mediated homologous direct repair. By breeding with tdTomato reporter mice and initiating
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Ciona, an ideal research organism to study the role of enhancers genesis (IF 1.5) Pub Date : 2023-11-27 Emma Kirsten Farley
Watching documentaries as a child, I became fascinated by how genomes encode the instructions to make all the cells of an organism. I studied Biochemistry at Oxford University as the subject seemed to provide a mechanistic understanding of living systems. During my time at Oxford, I completed my part II thesis (similar to a master's project) in Prof. Doug Higgs' lab. I learned about the regulation
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Ascidian gene regulation and bioadhesion genesis (IF 1.5) Pub Date : 2023-11-27 Ute Rothbächer
During my university studies in Munich, Germany, I explored Zoology, Biochemistry, Parasitology, and Immunology to focus on tumor biology and melanoma formation in my Diploma and PhD projects in Judy Johnson's lab. She encouraged, guided, and provided maximal freedom for scientific thinking and all basic methods. Cell specification and the plasticity of cell fate in response to surrounding signals
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Women in tunicate research: Pioneers of the past and their present legacy genesis (IF 1.5) Pub Date : 2023-11-27 Marie L. Nydam, Mary Beth Saffo, Anna Di Gregorio
The search for female scientists who pioneered the research on tunicates is hindered by the tradition of reporting only the first initials of authors' names on scientific publications using only the initials of their first names. While this practice has the theoretical merit of broadening the readership by preventing the possible bias that could be caused by the gender of the author(s) in some of the
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Searching for marine embryos, finding my path. genesis (IF 1.5) Pub Date : 2023-11-22 Anna Di Gregorio
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Generation and characterization of Chd7-iCreERT2-tdTomato mice genesis (IF 1.5) Pub Date : 2023-11-22 Zi'ang Han, Ze Wang, Zhuxi Huang, Weijun Feng
Heterozygous mutation of CHD7 gene causes a severe developmental disorder called CHARGE syndrome. In order to further explore the expression and function of Chd7 in vivo, we generated a Chd7-P2A-iCreERT2-P2A-tdTomato (in short, Chd7-CT-tdT) knockin mouse line using the CRISPR/Cas9 technology. The specificity and efficiency of two knockin genetic elements were validated. The Chd7-CT-tdT reporter gene
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Unveiling the impact of DNA methylation machinery: Dnmt1 and Dnmt3a in orchestrating oocyte development and cellular homeostasis genesis (IF 1.5) Pub Date : 2023-11-20 Fatma Uysal, Gozde Sukur, Nazlican Bozdemir, Ozgur Cinar
DNA methylation can be considered the most prominent in controlling the gene expression responsible for the balance between cell proliferation and cell death. In this study, we aimed to analyze the distinct contributions of Dnmt1 and Dnmt3a enzymes in oocyte maturation, survival, autophagy, reactive oxygen species (ROS) production, and compensation capacity of Dnmt3b and Dnmt3l enzymes in mouse oocytes
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1st International Symposium on Women in Tunicate Biology: Meeting report genesis (IF 1.5) Pub Date : 2023-11-20 Anna Di Gregorio, Marie L. Nydam
The 1st International Symposium on Women in Tunicate Biology was held online on March 28 and 29, 2023. This global symposium was attended by 45–50 researchers from countries including Austria, Brazil, India, Italy, Japan, New Zealand, Turkey, and the United States. Figure 1 is a photograph of some of the participants from the March 28th session. FIGURE 1 Open in figure viewerPowerPoint A worldwide
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Bop1 is required to establish precursor domains of craniofacial tissues genesis (IF 1.5) Pub Date : 2023-11-16 Stephanie Keer, Karen M. Neilson, Helene Cousin, Himani D. Majumdar, Dominique Alfandari, Steven L. Klein, Sally A. Moody
Bop1 can promote cell proliferation and is a component of the Pes1-Bop1-WDR12 (PeBoW) complex that regulates ribosomal RNA processing and biogenesis. In embryos, however, bop1 mRNA is highly enriched in the neural plate, cranial neural crest and placodes, and potentially may interact with Six1, which also is expressed in these tissues. Recent work demonstrated that during development, Bop1 is required
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Women researchers in tunicate biology at the Stazione Zoologica Anton Dohrn in Napoli. genesis (IF 1.5) Pub Date : 2023-11-15 Anna Di Gregorio,Annamaria Locascio,Filomena Ristoratore,Antonietta Spagnuolo
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Reflections on forty years as a female tunicate researcher. genesis (IF 1.5) Pub Date : 2023-11-09 Billie J Swalla
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EMT and primary ciliogenesis: For better or worse in sickness and in health genesis (IF 1.5) Pub Date : 2023-11-09 Camille E. Tessier, Aurore M. M. Dupuy, Thomas Pelé, Philippe P. Juin, Jacqueline A. Lees, Vincent J. Guen
Epithelial-mesenchymal transition (EMT) and primary ciliogenesis are two cell-biological programs that are essential for development of multicellular organisms and whose abnormal regulation results in many diseases (i.e., developmental anomalies and cancers). Emerging studies suggest an intricate interplay between these two processes. Here, we discuss physiological and pathological contexts in which
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A journey with ascidians in the pigmentation world. genesis (IF 1.5) Pub Date : 2023-11-08 Filomena Ristoratore
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Dynamics of transcription factors regulating ascidian embryogenesis. genesis (IF 1.5) Pub Date : 2023-11-07 Izumi Oda-Ishii
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Giovanna Zaniolo: An inspiring scientist, teacher, mentor, and colleague. Active: 1967–2012 genesis (IF 1.5) Pub Date : 2023-11-03 Lucia Manni, Chiara Anselmi
Giovanna Zaniolo (Figure 1a,b) is a researcher who has made significant contributions to the study of the tunicate Botryllus schlosseri in Italy, as part of a flourishing national community with a rich history dating back to the eighteenth century (Manni et al., 2019). Born in 1942 in Vicenza (Italy), Zaniolo enrolled in Biological Science at the University of Padova (Italy) in the early 1960s. She
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Stem cell-mediated development, regeneration, chimerism, and aging in the colonial chordate Botryllus schlosseri genesis (IF 1.5) Pub Date : 2023-10-27 Ayelet Voskoboynik
Stem cells are units of biological organization, responsible for tissue and organ development and regeneration. I study stem cell biology, aging, and the evolution of immunity using the colonial chordate Botryllus schlosseri as a model system. This organism is uniquely suited for this study because it is closely related to vertebrates, undergoes weekly cycles of stem cell mediated regeneration, is
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WntA and Wnt4 during the regeneration of internal organs in the holothurian Eupentacta fraudatrix genesis (IF 1.5) Pub Date : 2023-10-17 A. S. Girich
Over the past few years, it has been established that wnt genes are involved in the regenerative processes of holothurians. The wnt4 gene was identified as one of the most active genes in Eupentacta fraudatrix regeneration using differential gene expression analysis and qPCR of individual genes. Also, the wntA gene was found in holothurians, which is present only in invertebrates and can perform unique
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Antennapedia: The complexity of a master developmental transcription factor genesis (IF 1.5) Pub Date : 2023-10-13 Marco Rosales-Vega, Diana Reséndez-Pérez, Martha Vázquez
Hox genes encode transcription factors that play an important role in establishing the basic body plan of animals. In Drosophila, Antennapedia is one of the five genes that make up the Antennapedia complex (ANT-C). Antennapedia determines the identity of the second thoracic segment, known as the mesothorax. Misexpression of Antennapedia at different developmental stages changes the identity of the
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Engrailed: Pathological and physiological effects of a multifunctional developmental gene genesis (IF 1.5) Pub Date : 2023-10-13 Xiang Ma, Liang-Liang Zhao, Yi-Chun Yu, Yan Cheng
Engrailed-1 (EN1) is a developmental gene that encodes En1, a highly conserved transcription factor involved in regionalization during early embryogenesis and in the later maintenance of normal neurons. After birth, EN1 still plays a role in the development and physiology of the body; for example, it exerts a protective effect on midbrain dopaminergic (mDA) neurons, and loss of EN1 causes mDA neurons
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Sea squirts adventures genesis (IF 1.5) Pub Date : 2023-10-12 Noa Shenkar
Growing up by the shores of the Mediterranean Sea, I developed a strong curiosity regarding marine lifeforms, along with a strong desire to protect them. I focused my efforts on pursuing an academic career as a marine biologist, developing my own field of expertise. As Jerry Garcia of The Grateful Dead once said: “It's not enough to be the best at what you do; you must be perceived to be the only one
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Ascidian biodiversity in Brazil and other Latin American countries. genesis (IF 1.5) Pub Date : 2023-10-07 Rosana Moreira da Rocha
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Giuseppina Ortolani (1951-2009): A "grande dame" in ascidian embryology. genesis (IF 1.5) Pub Date : 2023-10-05 Fiorenza De Bernardi
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Tumor dormancy: EMT beyond invasion and metastasis genesis (IF 1.5) Pub Date : 2023-09-30 Patrick Aouad, Hazel M. Quinn, Adeline Berger, Cathrin Brisken
More than two-thirds of cancer-related deaths are attributable to metastases. In some tumor types metastasis can occur up to 20 years after diagnosis and successful treatment of the primary tumor, a phenomenon termed late recurrence. Metastases arise from disseminated tumor cells (DTCs) that leave the primary tumor early on in tumor development, either as single cells or clusters, adapt to new environments
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Two Ciona sister species are not just complex, but wonderful: A study of maternal mRNAs to safeguard life on earth. genesis (IF 1.5) Pub Date : 2023-09-28 Atsuko Sato
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Evidences of differential methylation in the genome during development in the cactophilic Drosophila species genesis (IF 1.5) Pub Date : 2023-09-26 Adriano S. Santos, Ester S. Ramos, Vera L. S. Valente-Gaiesky, Fábio de Melo Sene, Maura H. Manfrin
DNA methylation with 5-methylcytosine (5mC) has been reported in the genome of several eukaryotes, with marked differences between vertebrates and invertebrates. DNA methylation is poorly understood as its role in evolution in insects. Drosophila gouveai (cluster Drosophila buzzatii) presents larvae that develop obligatorily in necrotic tissues of cacti in nature, with the distribution of populations
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A long and winding but exciting road: Biodiversity, phylogenetic, and biogeographic relationships of ascidians in the Southwest Atlantic. genesis (IF 1.5) Pub Date : 2023-09-24 M Carla de Aranzamendi
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The story of my research with ascidians. genesis (IF 1.5) Pub Date : 2023-09-24 Fiorenza De Bernardi
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V. K. Meenakshi: The first lady of ascidian research in India. Active: 1994-present. genesis (IF 1.5) Pub Date : 2023-09-21 Jhimli Mondal
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“Beyond transcription: How post-transcriptional mechanisms drive neural crest EMT” genesis (IF 1.5) Pub Date : 2023-09-21 Mariann Guzman-Espinoza, Minyoung Kim, Cindy Ow, Erica J. Hutchins
The neural crest is a stem cell population that originates from the ectoderm during the initial steps of nervous system development. Neural crest cells delaminate from the neuroepithelium by undergoing a spatiotemporally regulated epithelial-mesenchymal transition (EMT) that proceeds in a coordinated wave head-to-tail to exit from the neural tube. While much is known about the transcriptional programs
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Diversity and distribution of ascidians genesis (IF 1.5) Pub Date : 2023-09-15 Lauren M. Stefaniak
My research focuses on the biodiversity and biogeography of ascidians, the mechanisms controlling the distribution of species and communities, and how human activities, such as shipping, coastal hardening, and climate changes can alter those distributions. Working with my masters and undergraduate student researchers, I use morphological and molecular taxonomy (Nydam et al., 2022; Reinhardt et al.
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Signals, grids, and geometry: In pursuit of understanding cell fate switches genesis (IF 1.5) Pub Date : 2023-09-15 Clare Hudson
After a degree in Biology at the University of Nottingham in the early 90s, I studied for a PhD focusing on frog endoderm formation with Prof. Hugh Woodland at the University of Warwick (Hudson et al., 1997). When I was looking for a lab to do postdoctoral studies, I was undecided whether to continue with Xenopus or switch to a different system. My fate was sealed at a postdoc interview with Patrick
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Regulatory mechanisms for sperm chemotaxis and flagellar motility. genesis (IF 1.5) Pub Date : 2023-09-12 Kogiku Shiba
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Beryl Iris Brewin (1910–1999)—Accomplished New Zealand scientist and promoter of marine science genesis (IF 1.5) Pub Date : 2023-08-30 Megan J. Wilson
EARLY LIFE AND EDUCATION OF BERYL BREWIN Beryl Brewin, born in December 1910, was the youngest of two daughters to Frank and Lucy Brewin. Her parents, despite not having the opportunity to attend high school themselves, instilled in Beryl a love for learning and education. They engaged in various activities together, fostering her interest in science. Beryl fondly recalled daily walks with her father
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Correlation between E-cadherin/β-catenin, Vimentin expression, clinicopathologic features and drug resistance prediction in naïve prostate cancer: A molecular and clinical study genesis (IF 1.5) Pub Date : 2023-08-30 Rahma Said, Javier Hernández-Losa, Amine Derouiche, Teresa Moline, Rosa Somoza Lopez de Haro, Skander Zouari, Ahlem Blel, Soumaya Rammeh, Slah Ouerhani
Although epithelial–mesenchymal markers play an important role in prostate cancer (PC), further research is needed to better understand their utility in diagnosis, cancer progression prevention, and treatment resistance prediction. Our study included 111 PC patients who underwent transurethral resection, as well as 16 healthy controls. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
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A gene regulatory system that directs gene expression at the 32-cell stage. genesis (IF 1.5) Pub Date : 2023-08-28 Miki Tokuoka
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The colonial tunicate Botryllus schlosseri: A key species for evolutionary developmental studies genesis (IF 1.5) Pub Date : 2023-08-28 Lucia Manni
I discovered tunicates while attending classes of Comparative Anatomy of Vertebrates as a Biology student at the University of Padua, Italy. I was fascinated by the evolutionary developmental (evo-devo) approach while I was analyzing vertebrate adaptations looking at their sister group, the simple tunicates. I graduated in 1988 and subsequently entered my university's graduate program in Bioscience
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Gretchen Lambert: taxonomist, explorer, and historian of the ascidian community. Active: 1968-present genesis (IF 1.5) Pub Date : 2023-08-21 Marie L. Nydam
Gretchen Lambert (nee Shapiro) was born in Duluth, Minnesota, USA in 1941, and spent a large portion of her childhood at a wilderness fly-in fishing and hunting resort in Ontario, Canada. Her interest in marine biology was ignited in the 1950s when she visited the Florida Keys with her father. Lambert earned her Bachelor of Arts from the University of Minnesota in Zoology in 1963 (magna cum laude)
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Building hearts for undergraduate research with tunicates. genesis (IF 1.5) Pub Date : 2023-08-16 Heather J Evans Anderson
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Ascidian evolution and ecology genesis (IF 1.5) Pub Date : 2023-08-16 Marie L. Nydam
I earned a Bachelor of Science degree in Evolution and Ecology (summa cum laude) from the University of California, Davis. At UC Davis, I worked with Jay Stachowicz to understand the recruitment patterns of fouling invertebrates and the role of predators in fouling community structure. At this time, I was introduced to ascidians because they were important members of the fouling community. I transitioned
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A temporally-restricted pattern of endothelial cell collagen 4 alpha 1 expression during embryonic development determined with a novel knockin Col4a1-P2A-eGFP mouse line genesis (IF 1.5) Pub Date : 2023-07-27 Nathaniel L. Lartey, Martijn van der Ent, Roxann Alonzo, Di Chen, Philip D. King
Classical collagen type IV comprising of a heterotrimer of two collagen IV alpha 1 chains and one collagen IV alpha 2 chain is the principal type of collagen synthesized by endothelial cells (EC) and is a major constituent of vascular basement membranes. In mouse and man, mutations in genes that encode collagen IV alpha 1 and alpha 2 result in vascular dysfunction. In addition, mutations in genes that
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Cover Image, Volume 61, Issue 3–4 genesis (IF 1.5) Pub Date : 2023-07-22 Vineet K. Maurya, Yan Ying, Denise G. Lanza, Jason D. Heaney, John P. Lydon
Cover illustration: The cover image is based on the Technical Note A CRISPR/Cas9-engineered mouse carrying a conditional knockout allele for the early growth response-1 transcription factor by Vineet K. Maurya et al., https://doi.org/10.1002/dvg.23515.
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Ascidians and their microbial symbionts genesis (IF 1.5) Pub Date : 2023-07-14 Susanna López-Legentil
I obtained my PhD in Zoology in 2005 studying the biology and ecology of Mediterranean ascidians at both the University of Barcelona (UB; Spain) and the University of Perpignan (France). In 2006, I obtained a Fulbright postdoctoral fellowship to continue my studies at the University of North Carolina Wilmington (UNCW; USA). I returned to Spain in 2010 and joined the faculty at UB. While there, my husband
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The molecular basis of ascidian whole body regeneration genesis (IF 1.5) Pub Date : 2023-07-14 Megan J. Wilson
I studied Biochemistry and Genetics for my BSc, due to my keen interest in molecular biology. For my Hons and then PhD I studied, delving into exploring a sigma factor: a gene regulatory protein involved in infection and virulence in the pathogenic bacteria Pseudomonas aeruginosa. My journey into developmental biology was rooted in personal curiosity. During my PhD, I sought to understand my brother's
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Resolving the mechanisms underlying epithelial-to-mesenchymal transition of the lateral plate mesoderm genesis (IF 1.5) Pub Date : 2023-07-13 Axel H. Newton, Craig A. Smith
Formation of the vertebrate limb buds begins with a localized epithelial-to-mesenchymal transition (EMT) of the somatic lateral plate mesoderm (LPM). While the processes that drive proliferation and outgrowth of the limb mesenchyme are well established, the fundamental mechanisms that precede this process and initiate EMT are less understood. In this review, we outline putative drivers of EMT of the
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Generation and characterization of a Dkk4-Cre knock-in mouse line genesis (IF 1.5) Pub Date : 2023-07-12 Houda Khatif, Hisham Bazzi
Ectodermal appendages in mammals, such as teeth, mammary glands, sweat glands and hair follicles, are generated during embryogenesis through a series of mesenchymal–epithelial interactions. Canonical Wnt signaling and its inhibitors are implicated in the early steps of ectodermal appendage development and patterning. To study the activation dynamics of the Wnt target and inhibitor Dickkopf4 (Dkk4)
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Tunicate Eco-Evo-Devo laboratory in IMS-METU genesis (IF 1.5) Pub Date : 2023-07-11 Arzu Karahan
I completed my undergraduate education in Atatürk University, Education Faculty, Biology Department. Then pursued my graduate education at the Biology Department of Mersin University. Both my master's and PhD theses were on the biological and population genetics features of various fish species. My initial encounter with tunicates dates back to my Postdoc at Israel Oceanographic and Limnologic Research
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A novel qPCR-based technique for identifying avian sex: An illustration within embryonic craniofacial bone genesis (IF 1.5) Pub Date : 2023-06-24 Claire J. Houchen, Maria Bergman-Gonzalez, Erin E. Bumann
Sex is a biological variable important to consider in all biomedical experiments. However, doing so in avian embryos can be challenging as sex can be morphologically indistinguishable. Unlike humans, female birds are the heterogametic sex with Z and W sex chromosomes. The female-specific W chromosome has previously been identified in chick using a species-specific polymerase chain reaction (PCR) technique
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Epithelial–mesenchymal plasticity in kidney fibrosis genesis (IF 1.5) Pub Date : 2023-06-22 Sudarat Hadpech, Visith Thongboonkerd
Epithelial–mesenchymal transition (EMT) is an important biological process contributing to kidney fibrosis and chronic kidney disease. This process is characterized by decreased epithelial phenotypes/markers and increased mesenchymal phenotypes/markers. Tubular epithelial cells (TECs) are commonly susceptible to EMT by various stimuli, for example, transforming growth factor-β (TGF-β), cellular communication
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Expanding EMC foldopathies: Topogenesis deficits alter the neural crest genesis (IF 1.5) Pub Date : 2023-06-15 Jonathan Marquez, Faiza Aslam, Mustafa K. Khokha
The endoplasmic reticulum (ER) membrane protein complex (EMC) is essential for the insertion of a wide variety of transmembrane proteins into the plasma membrane across cell types. Each EMC is composed of Emc1-7, Emc10, and either Emc8 or Emc9. Recent human genetics studies have implicated variants in EMC genes as the basis for a group of human congenital diseases. The patient phenotypes are varied
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Meeting report: Third Franco-Japanese developmental biology meeting “New Frontiers in developmental biology: Celebrating the diversity of life” genesis (IF 1.5) Pub Date : 2023-06-14 Oginuma Masayuki, Anne-Cécile Reymann
The French and Japanese Developmental Biology Societies, teaming up with Human Frontier Science Program, were eager to meet back in person in November 2022 in the lovely city of Strasbourg. Top scientists in the developmental biology field from France and Japan, but also from United States, United Kingdom, Switzerland or Germany shared their exciting science during the 4 days of this meeting. Core
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Spatio-temporal control of targeted gene expression in combination with CRISPR/Cas and Tet-On systems in Medaka genesis (IF 1.5) Pub Date : 2023-05-25 Daichi Kayo, Sayaka Kimura, Touko Yamazaki, Kiyoshi Naruse, Hideaki Takeuchi, Satoshi Ansai
Spatial and temporal control of transgene expression is a powerful approach to understand gene functions in specific cells and tissues. The Tet-On system is a robust tool for controlling transgene expression spatially and temporally; however, few studies have examined whether this system can be applied to postembryonic stages of Medaka (Oryzias latipes) or other fishes. Here, we first improved a basal