-
Screening and Identifying Reference Genes for Erythrocyte Production from Cord Blood CD34+ Cells Exposed to Hypoxia. DNA Cell Biol. (IF 3.1) Pub Date : 2023-11-23 Jun Xiao,Zhicai Li,Xiaowei Li,Huifen Lei,Fangyuan Meng,Cuiying Li
Cord blood (CB) CD34+ cells have the potential to be used to achieve artificial hematopoiesis because of their ability to expand and differentiate in multiple directions. However, the mechanism and molecular changes underlying such differentiation are still unclear. The differentiation of CB CD34+ cells is generally driven by subtle changes in gene expression. A crucial method for examining gene expression
-
Single Nucleotide Polymorphisms Mutation of Tropoelastin Gene Affects Tropoelastin mRNA and Elastin Expressions in Human Aortic Smooth Muscle Cells. DNA Cell Biol. (IF 3.1) Pub Date : 2023-11-21 Jie Yue,You-Fei Qi,Wen-Bo Zhang,Sa-Hua Liu,Hao Chen,Zhen-Zhen Li,Hong-Fei Wu
We aimed to explore the effects of single nucleotide polymorphisms (SNPs) in tropoelastin gene on tropoelastin mRNA and elastin expressions in human aortic smooth muscle cells (HASMCs). Two SNP loci, rs2071307 (G/A) and rs1785598 (G/C), were selected to construct recombinant lentivirus vectors carrying wild-type and mutant tropoelastin gene. Recombinant plasmids including pWSLV-02-ELN, pWSLV-02-ELN-mut1
-
Ferroptosis-Mediated Immune Microenvironment and Therapeutic Response in Inflammatory Bowel Disease. DNA Cell Biol. (IF 3.1) Pub Date : 2023-11-10 Haiming Tang,Peng Li,Xiutian Guo
Inflammatory bowel disease (IBD) is a chronic inflammatory disorder characterized by dysregulated immune responses in the gastrointestinal tract. One intriguing aspect of IBD is the potential involvement of ferroptosis, but the mechanism remains incompletely understood. In this study, 27 ferroptosis-related genes (FRGs) were identified differentially expressed between IBD and non-IBD control samples
-
A Novel Role of ARA70 in Regulating Ferritinophagy of RGCs During Retinal Ischemia Reperfusion. DNA Cell Biol. (IF 3.1) Pub Date : 2023-10-30 Xin Dong,Zijian Zhang,Nannan Yu,Huanqi Shi,Lili Lin,Yongsheng Hou
Although the contribution of ferroptosis, an iron-dependent cell death, to ischemia reperfusion (IR)-induced retinal injury has been reported before, to optimize therapeutic strategy, there is still an urgent need to identify potential candidates involved in this process. Androgen Receptor-Associated Protein of 70 kDa (ARA70) is a cargo receptor for ferritinophagy, and its role in retinal ferroptosis
-
-
Oridonin Promotes Apoptosis in Rheumatoid Arthritis Fibroblast-like Synoviocytes Through PERK/eIF2α/CHOP of Endoplasmic Reticulum Stress Pathway. DNA Cell Biol. (IF 3.1) Pub Date : 2023-10-20 Shoudi He,Changsheng Huang,Ning Tan,Jianyong Zhang
Oridonin (ORI), derived from Chinese herbs Rabdosia rubescens, has anti-inflammatory, proapoptotic, anticancer effects. Previous studies have found that ORI induces apoptosis in rheumatoid arthritis fibroblast synovial cells (RA-FLSs), but this mechanism is not clear. We will investigate the apoptosis mechanism of ORI on RA-FLSs. RA-FLSs were treated with various concentrations of ORI (0, 5, 10, 15
-
Analysis of the Imprinting Status and Expression of the MAGEL2 Gene During Initiation at Puberty in the Dolang Sheep. DNA Cell Biol. (IF 3.1) Pub Date : 2023-10-16 Yongjie Zhang,Zhiyuan Sui,Zhishuai Zhang,Chenguang Wang,Xiaojun Li,Feng Xing
Genomic imprinting refers to the expression of parent-specific genes in diploid mammalian cells. MAGEL2 gene is a maternally imprinted gene that has been identified in mice and humans and is associated with the onset of puberty. The purpose of this study was to investigate its imprinting status and its relationship with the onset of puberty in Dolang sheep. The sequence of 3734 bp cDNA of MAGEL2 in
-
LncRNA RP1-276N6.2 Expression and RP1-276N6.2 Gene Polymorphisms Contribute to the Risk of Coronary Artery Disease in Chinese Han Population. DNA Cell Biol. (IF 3.1) Pub Date : 2023-10-16 Lijuan Chen,Mingming Zhao,Mingsha Zhou,Jia Luo,Shan Li,Xing Liu,Zheng Cheng,Yang Zhuo,Weiqi Zeng,Zhiyu Zhang,Li Zhou
Long noncoding RNAs (lncRNAs) have been implicated in coronary artery disease (CAD) processes. However, the relationship between the gene polymorphisms of lncRNA RP1-276N6.2 as a novel molecule and susceptibility to CAD remains unclear. In our case-control study, 949 CAD patients and 892 healthy controls were genotyped using the TaqMan genotyping assay. The quantitative real-time polymerase chain reaction
-
The Molecular Mechanisms Involved in Axonal Degeneration and Retrograde Retinal Ganglion Cell Death. DNA Cell Biol. (IF 3.1) Pub Date : 2023-10-11 Zhaoyang Zuo,Ziyuan Zhang,Siming Zhang,Bin Fan,Guangyu Li
Axonal degeneration is a pathologic change common to multiple retinopathies and optic neuropathies. Various pathologic factors, such as mechanical injury, inflammation, and ischemia, can damage retinal ganglion cell (RGC) somas and axons, eventually triggering axonal degeneration and RGC death. The molecular mechanisms of somal and axonal degeneration are distinct but also overlap, and axonal degeneration
-
Key Subdomains of Cerebral Dopamine Neurotrophic Factor Regulate Its Protective Function in 6-Hydroxydopamine-Lesioned PC12 Cells. DNA Cell Biol. (IF 3.1) Pub Date : 2023-10-09 Hao Liu,Haibin Dong,Chunxiao Wang,Wenjuan Jia,Guangqiang Wang,Hua Wang,Lin Zhong,Lei Gong
Cerebral dopamine neurotrophic factor (CDNF) is a unique neurotrophic factor (NTF) that has shown significant neuroprotective and neurorestorative functions on midbrain dopaminergic neurons. The secondary structure of human CDNF protein contains eight α-helices. We previously found that two key helices, α1 and α7, regulated the intracellular trafficking and secretion of CDNF protein in different manners
-
Homozygous Mutations in GDAP1 and MFN2 Genes Resulted in Autosomal Recessive Forms of Charcot-Marie-Tooth Disease in Consanguineous Pakistani Families. DNA Cell Biol. (IF 3.1) Pub Date : 2023-10-05 Muhammad Asif,Chien-Chun Chiou,Malik Fiaz Hussain,Manzoor Hussain,Zureesha Sajid,Muhammad Gulsher,Afifa Raheem,Adil Khan,Nasreen Nasreen,Andrzej Kloczkowski,Mubashir Hassan,Furhan Iqbal,Chien-Chin Chen
Charcot-Marie-Tooth disease (CMT) is a heritable neurodegenerative disease of peripheral nervous system diseases in which more than 100 genes and their mutations are associated. Two consanguineous families Dera Ghazi Khan (PAK-CMT1-DG KHAN) and Layyah (PAK-CMT2-LAYYAH) with multiple CMT-affected subjects were enrolled from Punjab province in Pakistan. Basic epidemiological data were collected for the
-
Identification of A Novel Variant of Filamin A Destroying the Attraction Between Benzene Rings and Sulfhydryl in Developmental Dysplasia of the Hip. DNA Cell Biol. (IF 3.1) Pub Date : 2023-10-01 Yi-Lei Lu,Qin Wang,Min Wang,Si-Hua Chang,Ji-Qiang He,Rong Xiang,Ju-Yu Tang,Jie-Yuan Jin
Developmental dysplasia of the hip (DDH), characterized by acetabular deformity that manifests from loose ligaments to complete dislocation of the hip, can cause notable pain and dysfunction and lead to hip dislocation, secondary fractures, scoliosis, and osteoarthritis of hip. Variants in FLNA may produce a spectrum of malformations in multiple organs, especially the skeleton. This study aimed to
-
Astragaloside IV Inhibited Podocyte Pyroptosis in Diabetic Kidney Disease by Regulating SIRT6/HIF-1α Axis. DNA Cell Biol. (IF 3.1) Pub Date : 2023-09-25 Mingyu Zhang,Wenyuan Liu,Yuxiang Liu,Ziyuan Zhang,Yaling Hu,Dalin Sun,Sufen Li,Jingai Fang
To investigate the effect of astragaloside IV (AS) on podocytes pyroptosis in diabetic kidney disease (DKD). Forty male Sprague-Dawley rats were randomly divided into normal group (n = 10) and model group (n = 30). Rats in model group were intraperitoneally injected streptozotocin (60 mg/kg) for 3 days to induce DKD. Then rats were divided into DKD group, AS group, and UBCS group. The AS group was
-
Plasma β-1,3 Glucan Binding Protein Mediated Opsono-Phagocytosis by Hemocytes In Vitro of Marine Mussel Perna viridis. DNA Cell Biol. (IF 3.1) Pub Date : 2023-09-11 A Johnpaul,M Arumugam
We have shown in the past decade, for the first time in a bivalve mollusc, detection, isolation, and purification of β-1,3 glucan binding protein (β-GBP) in the plasma of the marine mussel Perna viridis and demonstrated its role in a nonself-induced activation of plasma prophenoloxidase system. In this study, we present evidence for its ability to function as an opsonin during phagocytosis of trypsinized
-
Antitumor Effects of β-Elemene Through Inducing Autophagy-Mediated Apoptosis in Ewing Sarcoma Family Tumor Cells. DNA Cell Biol. (IF 3.1) Pub Date : 2023-08-22 Ting Zhang,Tianhua Zhang,Chuanzhou Gao,Sajid Jalal,Ruqiang Yuan,Hongming Teng,Cong Li,Lin Huang
Ewing sarcoma family tumors (ESFTs) are a group of aggressive tumors mainly affecting children and young people. A compound derived from Curcuma wenyujin plant or lemon grass, β-elemene, has exhibited antitumor effects to ESFT cells, the mechanism of which remains to be clarified further. Autophagy is involved in the antitumor effects of various drugs, whereas the role of autophagy in the antitumor
-
Altered G-Protein Transduction Protein Gene Expression in the Testis of Infertile Patients with Nonobstructive Azoospermia. DNA Cell Biol. (IF 3.1) Pub Date : 2023-08-22 Danial Hashemi Karoii,Hossein Azizi,Thomas Skutella
Recent studies have shown that several members of the G-protein-coupled receptors (GPCR) superfamily play crucial roles in the maintenance of ion-water homeostasis of the sperm and Sertoli cells, development of the germ cells, formation of the blood barrier, and maturation of sperm. The GPCR, guanyl-nucleotide exchange factor, membrane traffic protein, and small GTPase genes were analyzed by microarray
-
LncRNA Gm44206 Promotes Microglial Pyroptosis Through NLRP3/Caspase-1/GSDMD Axis and Aggravate Cerebral Ischemia-Reperfusion Injury. DNA Cell Biol. (IF 3.1) Pub Date : 2023-08-11 Liangliang Yang,Yang Gao,Jinlong Huang,Hantao Yang,Puyuan Zhao,Chen Li,Zhigang Yang
Inhibition of the inflammatory response triggered by microglial pyroptosis inflammatory activation may be one of the effective ways to alleviate cerebral ischemia-reperfusion injury, the specific mechanism of which remains unclear. In this study, BV-2 microglia with or without oxygen-glucose deprivation/reoxygenation (OGD/R) or long noncoding RNA (lncRNA) Gm44206 knockdown were used as cell models
-
De Novo Mutation in KRT1 Leads to Epidermolytic Palmoplantar Keratoderma: from Chinese Traditional Treatment to Prenatal Diagnosis Using Whole-Exome Sequencing-Plus. DNA Cell Biol. (IF 3.1) Pub Date : 2023-08-10 Mengdi Ge,Chunmin Ji,Huanzhen Li,Huan Huang
Congenital skin disorders are a class of complex genetic diseases that are difficult to diagnose and treat. We developed trio whole-exome sequencing-plus (WES-plus) for detecting de novo mutations and evaluated the use of traditional Chinese medicine (TCM) for treating congenital skin disorders. In this study, we successively performed panel-based next-generation sequencing (NGS) and Trio WES-plus
-
Genotypic Analysis of COL4A1 Gene in Diabetic Nephropathy and Type 2 Diabetes Mellitus Patients: A Comparative Genetic Study. DNA Cell Biol. (IF 3.1) Pub Date : 2023-08-04 Neha Shukla,Shivani Kumari,Poornima Verma,Atar Singh Kushwah,Monisha Banarjee,S N Sankhwar,Aneesh Srivastava,M S Ansari,Naveen Kumar Gautam
Diabetic nephropathy (DN) is specified by microalbuminuria, glomerular lesions, and renal fibrosis leading to end-stage renal disease. The pathophysiology of DN is multifactorial as a result of gene-environment interaction. Clinical studies suggested that gene mutations affect various pathways involved in DN, including extracellular matrix (ECM). During chronic hyperglycemia, collagen type-4-mediated
-
RNA-Sequencing Analysis Indicates That N-Cadherin Promotes Prostate Cancer Progression by the Epigenetic Modification of Key Genes. DNA Cell Biol. (IF 3.1) Pub Date : 2023-08-04 Yongjun Quan,Hao Ping,Mingdong Wang,Xiaodong Zhang
N-cadherin (cadherin-2 [CDH2]) is widely known as the promoter of prostate cancer (PCa) invasion and castration resistance. However, the biological mechanism of N-cadherin in PCa progression is unclear. In this study, we overexpressed N-cadherin in LNCaP cells and downregulated N-cadherin in PC3 cells by lentiviral transduction. Then, differentially expressed genes (DEGs) and dysregulated biological
-
Lipid Metabolic Regulatory Crosstalk Between Cancer Cells and Tumor-Associated Macrophages. DNA Cell Biol. (IF 3.1) Pub Date : 2023-08-03 Shu Liu,Ying Ying Shen,Li Yang Yin,Jianghua Liu,Xuyu Zu
In the tumor microenvironment, tumor-associated macrophages (TAMs) are one of the most abundant cell populations, playing key roles in tumorigenesis, chemoresistance, immune evasion, and metastasis. There is an important interaction between TAMs and cancer cells: on the one hand, tumors control the function of infiltrating macrophages, contributing to reprogramming of TAMs, and on the other hand, TAMs
-
Novel Long Noncoding RNAs, LINC01093 and MYLK-AS1, Serve as Potential Diagnostic and Prognostic Biomarkers or Hepatocellular Carcinoma. DNA Cell Biol. (IF 3.1) Pub Date : 2023-08-01 Yanming Qin,Xin Tu,Meifang Huang,Caifang Ma,Qiongqing Huang,Qiqi Huang,Hong Shu,Chao Ou
Hepatocellular carcinoma (HCC) is one of the most fatal human malignancies worldwide. In this research, we aimed to identify long noncoding RNAs (lncRNAs) as biomarkers for HCC diagnosis and prognosis. lncRNA expression profiles were obtained from Gene Expression Omnibus and The Cancer Genome Atlas databases. The differentially expressed lncRNAs between HCC and adjacent tissues were analyzed with bioinformatic
-
Downregulation of miR-92a in Decidual Stromal Cells Suppresses Migration Ability of Trophoblasts by Promoting Macrophage Polarization. DNA Cell Biol. (IF 3.1) Pub Date : 2023-08-01 Huansheng Zhou,Hui Wang,Xiaohan Liu,Bei Liu,Yanci Che,Rendong Han
Preeclampsia (PE) is a severe pregnancy complication that accounts for about 14% of maternal deaths. Its clinical manifestations commonly include hypertension and proteinuria. However, it is largely limited in understanding its pathogenetic mechanism. In this study, we used bioinformatics to compare differential gene expressions in decidual stromal cells from PE patients and healthy donors. The result
-
Association of MMP7 T > C Gene Variant (rs10502001) and Expression in Chronic Obstructive Pulmonary Disease. DNA Cell Biol. (IF 3.1) Pub Date : 2023-08-01 Saurabh Kumar,Suchit Swaroop,Akancha Sahu,Surya Kant,Monisha Banerjee
Patients with chronic obstructive pulmonary disease (COPD) have obstructed airflow through their lungs. Single nucleotide polymorphisms in matrix metalloproteinases (MMPs) genes and the risk of COPD have been the subject of numerous studies, with conflicting results. This investigation was conducted to determine whether the MMP7 (T>C) gene variant (rs10502001) was associated with an increased risk
-
The Role of Fibroblast Growth Factor Signaling in Somitogenesis. DNA Cell Biol. (IF 3.1) Pub Date : 2023-07-18 Angad Singh Chandel,Matthew Stocker,Ertuğrul M Özbudak
Fibroblast growth factor (FGF) signaling is conserved from cnidaria to mammals (Ornitz and Itoh, 2022) and it regulates several critical processes such as differentiation, proliferation, apoptosis, cell migration, and embryonic development. One pivotal process FGF signaling controls is the division of vertebrate paraxial mesoderm into repeated segmented units called somites (i.e., somitogenesis). Somite
-
Genome-Wide Association Studies to Drug: Identifying Retinoic Acid Metabolism Blocking Agents to Suppress Mechanoflammation in Osteoarthritis. DNA Cell Biol. (IF 3.1) Pub Date : 2023-07-07 Linyi Zhu,Tonia L Vincent
Osteoarthritis (OA) is a highly prevalent debilitating joint disease for which there are currently no licensed disease-modifying treatments. The pathogenesis of OA is complex, involving genetic, mechanical, biochemical, and environmental factors. Cartilage injury, arguably the most important driving factor in OA development, is able to activate both protective and inflammatory pathways within the tissue
-
Rosalind Franklin Society Proudly Announces the 2022 Award Recipient for DNA and Cell Biology. DNA Cell Biol. (IF 3.1) Pub Date : 2023-07-01 Carolin M Lieber
-
Aquaporin 1 Facilitates Ferroptosis, M1 Polarization, Mitochondrial Dysfunction, and Autophagy Damage on Lipopolysaccharide-Induced Macrophage Through Down-Regulation of P53 Signaling Pathway. DNA Cell Biol. (IF 3.1) Pub Date : 2023-06-28 Wuyang Lv,Lei Liang,Dongyang Liu,Cuicui Li,Liao Jia,Yingyu Jin
This study was designed to investigate the role of aquaporin 1 (AQP1) in ferroptosis, macrophage polarization, mitochondrial dysfunction, and impaired autophagy of lipopolysaccharide (LPS)-stimulated RAW264.7 cells and explored the underlying mechanisms. Si-AQP1-mediated AQP1 silencing RAW264.7 cells was constructed. Si-P53-mediated P53 silencing or pcDNA-P53 overexpression RAW264.7 cells was constructed
-
Hypomethylation of Long Interspersed Nucleotide Elements and Aldehyde Dehydrogenase in Patients of Alcohol Use Disorder with Cirrhosis. DNA Cell Biol. (IF 3.1) Pub Date : 2023-06-27 Bhagyalakshmi Shankarappa,Jayant Mahadevan,Pratima Murthy,Meera Purushottam,Biju Viswanath,Sanjeev Jain,Harshad Devarbhavi,Ashok Mysore Visweswariah
Alcohol use disorder (AUD) and cirrhosis are key outcomes of excessive alcohol use, and a genetic influence in these outcomes is increasingly recognized. While 80-90% of heavy alcohol users show evidence of fatty liver, only 10-20% progress to cirrhosis. There is currently no clear understanding of the causes of this difference in progression. The aim of this study is to evaluate genetics and epigenetics
-
Loss of circIGF1R Suppresses Cardiomyocytes Proliferation by Sponging miR-362-5p. DNA Cell Biol. (IF 3.1) Pub Date : 2023-06-22 Jun-Hui Zeng,Hong-Ji Li,Kun Liu,Chi-Qian Liang,Hai-Yan Wu,Wu-Yun Chen,Ming-Hui Tang,Wan-Ling Zhao,Dong-Qing Cai,Xu-Feng Qi
Circular RNAs (circRNAs) are generally formed by the back-splicing of precursor mRNA. Increasing evidence implicates the important role of circRNAs in cardiovascular diseases. However, the role of circ-insulin-like growth factor 1 receptor (circIGF1R) in cardiomyocyte (CM) proliferation remains unclear. Here, we investigated the potential role of the circIGF1R in the proliferation of CMs. We found
-
Targeting Hub Genes Involved in Muscle Injury Induced by Jumping Load Based on Transcriptomics. DNA Cell Biol. (IF 3.1) Pub Date : 2023-07-28 Xiaolan Shi,Yijie Wang,Haitao Liu,Rui Han
The purpose of this study was to find hub genes that may play key roles in skeletal muscle injury induced by jumping load. Twelve female Sprague Dawley rats were divided into the normal control (NC) group and the jumping-induced muscle injury (JI) group. After 6 weeks of jumping, transmission electron microscopy, hematoxylin-eosin staining, transcriptomics sequencing and genes analysis, interaction
-
lncRNA MIR31HG Regulates Proliferation and Migration by Targeting Matrix Gla Protein in Nonsyndromic Cleft Lip With or Without Cleft Palate. DNA Cell Biol. (IF 3.1) Pub Date : 2023-06-15 Xiaofeng Li,Xinze Xu,Luwei Liu,Yu Tian,Yue Gao,Guirong Zhu,Shu Lou,Weijie Zhong,Dandan Li,Yongchu Pan
Nonsyndromic cleft lip with or without cleft palate (NSCL/P) is a common craniofacial birth defect with complex etiologies. Recently, the dysregulation of long noncoding RNAs (lncRNAs) has been implicated in many developmental diseases, including NSCL/P. However, the functions and mechanisms of lncRNAs in NSCL/P have not been fully elucidated. In this study, we found that lncRNA MIR31HG in NSCL/P patients
-
Investigation of Properties of the Mitochondrial Permeability Transition Pore Using Whole-Mitoplast Patch-Clamp Technique. DNA Cell Biol. (IF 3.1) Pub Date : 2023-06-13 Maria A Neginskaya,Evgeny V Pavlov
The mitochondrial permeability transition pore (mPTP) is a channel in the mitochondrial inner membrane that is activated by excessive calcium uptake. In this study, we used a whole-mitoplast patch-clamp approach to investigate the ionic currents associated with mPTP at the level of the whole single mitochondrion. The whole-mitoplast conductance was at the level of 5 to 7 nS, which is consistent with
-
Effect of Low Dietary Folate on Mouse Spermatogenesis and Spindle Assembly Checkpoint Dysfunction May Contribute to Folate Deficiency-Induced Chromosomal Instability in Cultured Mouse Spermatogonia. DNA Cell Biol. (IF 3.1) Pub Date : 2023-06-08 Huanhuan Ren,Kaixian Wang,Zirui Liu,Xuansheng Zhong,Meng Liang,Yaping Liao
Folate, as the initial substrate in one-carbon metabolism, is involved in the synthesis of important substances such as DNA, RNA, and protein. Folate deficiency (FD) is associated with male subfertility and impaired spermatogenesis, yet the underlying mechanisms are poorly understood. In the present study, we established an animal model of FD to investigate the effect of FD on spermatogenesis. GC-1
-
Bioinformatics Analysis Reveals the Vital Role of AKR1B1 in Immune Infiltration and Clinical Outcomes of Gastric Cancer. DNA Cell Biol. (IF 3.1) Pub Date : 2023-06-07 Zhiyue Zhao,Zhibin Hao,Zheng Zhang,Xianbao Zhan
Infiltrated immune cells are an important constitute of tumor microenvironment, which exert complex effects on gastric cancer (GC) pathogenesis and progression. By using weighted gene co-expression network analysis, integrating the data from The Cancer Genome Atlas-stomach adenocarcinoma and GSE62254, we identify Aldo-Keto Reductase Family 1 Member B (AKR1B1) as a hub gene for immune regulation in
-
Mitochondrial Dynamics of Podocyte in Various International Society of Nephrology/Renal Pathology Society Class Lupus Nephritis Patients. DNA Cell Biol. (IF 3.1) Pub Date : 2023-05-30 Qijiao Wei,Qing Yan,Shengli Zhang,Fei Gao,Zhihan Chen,He Lin,Li Sun
Morphological changes of podocyte mitochondria are observed in patients with mitochondrial cytopathy and nephrotic syndrome. However, whether mitochondrial dynamics involved in podocyte in lupus nephritis (LN) is still not clear. This study aims to investigate the associations between mitochondrial morphology and podocyte lesions and laboratory and pathological features in LN. The foot process width
-
FTO Promotes the Stemness of Gastric Cancer Cells. DNA Cell Biol. (IF 3.1) Pub Date : 2023-05-25 Mengqing Li,Xuan Wu,Guan Li,Guoqing Lv,Shubin Wang
The full name of the FTO gene is fat mass and obesity-associated gene. In recent years, it has also been found that FTO is involved in m6A demethylation and regulates the progression of multiple cancers, including gastric cancer. The cancer stem cell theory argues that cancer stem cells are key factors in cancer metastasis, and inhibiting the expression of stemness genes is a good method to inhibit
-
The Novel Circular RNA circTRIO Silence Inhibits the Progression of Laryngeal Squamous Cell Carcinoma. DNA Cell Biol. (IF 3.1) Pub Date : 2023-05-22 Jing Wang,Shujin He,Dingding Wang,Yanlei Jing,Wenping Shen,Anqi Huang,Zhe Zhou,Honggang Liu
Laryngeal squamous cell carcinoma (LSCC) is the most common and prevalent malignant tumor in head and neck squamous cell carcinoma. Recent studies have shown that circular RNAs (circRNAs) play a vital role in cancer development, but their specific role in the tumorigenesis and development of LSCC remains unclear. We selected five pairs of LSCC tumor tissues and paracancerous tissues for RNA sequencing
-
Selenium Nanoparticles Show Anticancer Activity Through Regulation of HIF-1α and HIF-2α Under Hypoxic Condition in Liver Cancer Cells. DNA Cell Biol. (IF 3.1) Pub Date : 2023-05-22 Sancharan Acharya,Subramaniyam Nithyananthan,Chinnasamy Thirunavukkarasu
Tumor microenvironment has significant influence in therapeutic response and clinical outcome. Combination therapy is more effective in cancer treatment compared with monotherapy. Any chemical or drug that targets tumor microenvironment pathway, will be a boon to combination cancer chemotherapy. Combination therapy through micronutrient may have added advantage in clinical applications. Selenium (Se)
-
Virus-Induced Lysis of Tumor and Other Pathogenic Unicellular Entities and Its Potential to Treat Leishmaniasis. DNA Cell Biol. (IF 3.1) Pub Date : 2023-05-16 Janaina Fernandes
This article is focused on the main pathways used by viruses to achieve infection and lysis of unicellular eukaryotes described as pathogenic for multicellular organisms. In light of the recent discussions on how tumor cells exhibit unicellular behavior, highly malignant cells can be considered as another unicellular pathogenic entity, but with endogenous origin. Thus, a comparative panel of viral
-
Effect of the Mitogen-Activated Protein Kinase Pathway on the Erastin-Induced Ferroptosis of Molt-4 Cells. DNA Cell Biol. (IF 3.1) Pub Date : 2023-05-04 Nana Liu,Ge Liu,Haihong Jiang,Jing Yu,Yunqin Jin,Hong Wang
The role of ferroptosis in human acute lymphoblastic leukemia and its possible molecular mechanisms of action are still unknown. In this study, harvested Molt-4 cells were exposed to different concentrations of erastin, and their proliferation capacity was tested by using the cell counting kit-8 assay. Lipid peroxidation levels were detected through flow cytometry. Mitochondrial alterations were observed
-
Endoplasmic Reticulum Stress: A Key Regulator of Cardiovascular Disease. DNA Cell Biol. (IF 3.1) Pub Date : 2023-05-03 Zhao Chen,Shi-Liang Zhang
The problems associated with economic development and social progress have led to an increase in the occurrence of cardiovascular diseases (CVDs), which affect the health of an increasing number of people and are a leading cause of disease and population mortality worldwide. Endoplasmic reticulum stress (ERS), a hot topic of interest for scholars in recent years, has been confirmed in numerous studies
-
ZNF8-miR-552-5p Axis Modulates ACSL4-Mediated Ferroptosis in Hepatocellular Carcinoma. DNA Cell Biol. (IF 3.1) Pub Date : 2023-04-26 Hao Yang,Wensheng Sun,Tao Bi,Jiahao Sun,Zhihua Lu,Jie Li,Honglong Wei
Hepatocellular carcinoma (HCC) is a common malignancy that is associated with poor prognosis in humans. Despite the development of targeted drugs, overall survival remains a significant challenge, and new therapeutic strategies are urgently needed. The aim of this study was to investigate the function of miR-552-5p in ferroptosis and the underlying mechanism, as well as to explore novel strategies
-
Mycobacterium tuberculosis PE8 (Rv1040c) Promotes the Intracellular Survival of Recombinant Mycobacterium by Regulating Host Inflammatory Cytokines and Inhibiting Cell Late Apoptosis. DNA Cell Biol. (IF 3.1) Pub Date : 2023-04-18 Tao Xu,Chutong Wang,Minying Li,Meili Yuan,Jing Wei,Baiqing Li,Zhongqing Qian,Ting Wang,Xiaojing Wang,Hongtao Wang
Tuberculosis is an important chronic and often fatal infectious disease mainly caused by the bacterium Mycobacterium tuberculosis (Mtb). Mtb is one of the most successful pathogens that harbors several potential virulence factors not found in nonpathogenic mycobacteria. As the Mtb cell envelope is closely associated with its virulence and resistance, it is very important to understand the cell envelope
-
MicroRNA-126 Regulates Thrombosis Through Endothelial Progenitor Cells. DNA Cell Biol. (IF 3.1) Pub Date : 2023-04-10 Qian Xiao,Dan Wang,Yingda Sheng,Jing Huang,Xiaoqin Ha
Thrombosis is a common problem with potentially severe consequences. Endothelial progenitor cells (EPCs) show great potential as a thrombosis therapy due to their angiogenesis-promoting, thrombus-relieving, and anticoagulant functions. However, cell therapies present more clinical challenges than small molecule solutions. MicroRNAs (miRNAs) are small noncoding single-stranded RNAs with wide-ranging
-
Oncolytic Reoviruses: Can These Emerging Zoonotic Reoviruses Be Tamed and Utilized? DNA Cell Biol. (IF 3.1) Pub Date : 2023-04-04 Zhen Yun Siew,Alson Loh,Sharrada Segeran,Pooi Pooi Leong,Kenny Voon
Orthoreovirus is a nonenveloped double-stranded RNA virus under the Reoviridae family. This group of viruses, especially mammalian orthoreovirus (MRV), are reported with great therapeutic values due to their oncolytic effects. In this review, the life cycle and oncolytic effect of MRV and a few emerging reoviruses were summarized. This article also highlights the challenges and strategies of utilizing
-
A Deletion in Duchenne Muscular Dystrophy Gene Found Through Whole Exome Sequencing in Iran. DNA Cell Biol. (IF 3.1) Pub Date : 2023-03-30 Saman Ameri-Mahabadi,Ali Nikfar,Mojdeh Mansouri,Hossein Chiti,Gita Fatemi Abhari,Negin Parsamanesh
Duchenne muscular dystrophy (DMD) is a severe progressive X-linked neuromuscular illness that affects movement through mutations in dystrophin gene. The mutation leads to insufficient, lack of, or dysfunction of dystrophin. The cause of DMD was determined in an Iranian family. Exome sequencing was carried out along with a complete physical examination of the family. In silico methods were applied to
-
NF1-Related MicroRNA Gene Polymorphisms and the Susceptibility to Soft Tissue Sarcomas: A Case-Control Study. DNA Cell Biol. (IF 3.1) Pub Date : 2023-03-29 Peng Zhang,Lingling Huang,Xinling Li,Fulan Hu,Xiaoying Niu,Yang Sun,Weitao Yao,Wen Tian
Soft tissue sarcomas (STS) are rare malignant tumors of mesenchymal origin, which are easy to metastasize and relapse and are a great threat to human health. In our previous study, the abnormal expression of neurofibromin 1 (NF1) is observed in tumor tissue of STS, and the NF1 gene is regulated by miRNAs. The study aimed to assess the association between NF1-related miRNA gene polymorphisms and the
-
Correlation Analysis of CTSB Promoter Polymorphism and Function in Patients with Dilated Cardiomyopathy. DNA Cell Biol. (IF 3.1) Pub Date : 2023-03-28 Yu Zhou,Shuang Gao,Liangcai Ding,Han Yan,Shuchao Pang,Bo Yan
Dilated cardiomyopathy (DCM) is caused by a combination of genetic susceptibility and environmental factors. Cathepsin B affects the pathogenesis of DCM; however, its molecular mechanism is still unclear. In this study, we examined the association of rare CTSB variants with the occurrence of DCM. This case-control study involved 394 participants: 142 patients with DCM and 252 healthy controls. DNA
-
-
A Single-Cell RNA-Sequencing Analysis of Distinct Subsets of Synovial Macrophages in Rheumatoid Arthritis. DNA Cell Biol. (IF 3.1) Pub Date : 2023-03-20 Xiaoyu Li,Hao Sun,Hao Li,Deng Li,Zhiqing Cai,Jie Xu,Ruofan Ma
The polarization states and molecular signatures of macrophages in the synovium of patients with rheumatoid arthritis (RA) are not well understood. We aimed to identify specific subpopulations of macrophages and their features in RA synovium thereby providing a theoretical basis for treatment of RA. Single-cell RNA sequencing (scRNA-seq) was used to identify cell subsets and their gene signatures in
-
E3 Ubiquitin Ligase Constitutive Photomorphogenic 1 Regulates Differentiation and Inflammation via MAPK Signaling Pathway in Rabbit Articular Chondrocytes. DNA Cell Biol. (IF 3.1) Pub Date : 2023-03-20 Young Seok Eom,Byung Su Ko,Fahad Hassan Shah,Song Ja Kim
Constitutive photomorphogenic 1 (COP1), is an E3 ubiquitin ligase that plays a role in the regulation of various cellular processes including cell growth, differentiation, and survival in mammals. In certain conditions such as overexpression or loss of function, COP1 acts either as an oncogenic protein or as a tumor suppressor by targeting specific proteins for ubiquitination-mediated degradation.
-
Cancer-Related Unconventional Protein Secretion: A New Role of the Endoplasmic Reticulum. DNA Cell Biol. (IF 3.1) Pub Date : 2023-03-17 Kohji Yamada,Kiyotsugu Yoshida
Unconventional protein secretion (UPS) is a crucial mechanism controlling the localization of cytosolic proteins lacking signal peptides and is implicated in inflammation, neurodegenerative diseases, and cancer. Several previous studies on immune cells have demonstrated the mechanisms of UPS. In cancer, the active secretion of several cytosolic proteins, including PKCδ and nucleolin, has been described
-
Clinical Diagnostic Values of Transfer RNA-Derived Fragment tRF-41-YDLBRY73W0K5KKOVD and its Effects on the Growth of Gastric Cancer Cells. DNA Cell Biol. (IF 3.1) Pub Date : 2023-03-03 Yao Wang,Zhe Li,Qiuyan Weng,Ying Zheng,Yifan Lin,Junming Guo,Guoliang Ye
Gastric cancer (GC) is a serious disease with high mortality and poor prognosis. It is known that tRNA halves play key roles in the progression of cancer. This study explored the function of the tRNA half tRF-41-YDLBRY73W0K5KKOVD in GC. Quantitative real-time reverse transcription-polymerase chain reaction was used to measure RNA levels. The level of tRF-41-YDLBRY73W0K5KKOVD in GC cells was regulated
-
Aberrant DNA Methylation Patterns of Deleted in Liver Cancer 1 Isoforms in Hepatocellular Carcinoma. DNA Cell Biol. (IF 3.1) Pub Date : 2023-03-01 Junhai Pan,Duguang Li,Xiaoxiao Fan,Jiaxi Cheng,Shengxi Jin,Peng Chen,Hui Lin,Yirun Li
Hepatocellular carcinoma (HCC), a common primary liver cancer, is the third leading cause of death worldwide. DNA methylation changes are common in HCC and have been studied to be associated with hepatocarcinogenesis. In our study, we used the MassARRAY® EpiTYPER technology to investigate the methylation differences of deleted in liver cancer 1 (DLC1) (isoform 1 and 3) promoter between HCC tissues
-
Global Long Noncoding RNA Expression Profiling of MSTN and FGF5 Double-Knockout Sheep Reveals the Key Gatekeepers of Skeletal Muscle Development. DNA Cell Biol. (IF 3.1) Pub Date : 2023-03-01 Mingming Chen,Di Lian,Yan Li,Yue Zhao,Xueling Xu,Zhimei Liu,Jinlong Zhang,Xiaosheng Zhang,Sujun Wu,Shiyu Qi,Shoulong Deng,Kun Yu,Zhengxing Lian
Improving livestock and poultry growth rates and increasing meat production are urgently needed worldwide. Previously, we produced a myostatin (MSTN) and fibroblast growth factor 5 (FGF5) double-knockout (MF-/-) sheep by CRISPR Cas9 system to improve meat production, and also wool production. Both MF-/- sheep and the F1 generation (MF+/-) sheep showed an obvious "double-muscle" phenotype. In this study
-
Expression of the Ovine Gene and the Relationship Between Its Polymorphism and Feed Efficiency Traits. DNA Cell Biol. (IF 3.1) Pub Date : 2023-02-24 Xiwen Zeng,Weimin Wang,Deyin Zhang,Xiaolong Li,Yukun Zhang,Yuan Zhao,Liming Zhao,Jianghui Wang,Dan Xu,Jiangbo Cheng,Wenxin Li,Bubo Zhou,Changchun Lin,Xiaobin Yang,Rui Zhai,Zongwu Ma,Jia Liu,Panpan Cui,Xiuxiu Weng,Weiwei Wu,Xiaoxue Zhang,Wenxin Zheng
In the mutton industry, feed efficiency traits have the greatest influence on the economic benefits of sheep raised in housing conditions. In this study, quantitative real-time PCR (qRT-PCR), Sanger sequencing, and KASPar methods were used to detect the expression levels of the B cell scaffold protein with ankyrin repeats 1 (BANK1) gene and the relationship between its polymorphism and feed efficiency
-
Analysis of Methylation and mRNA Expression of Lin28B Gene Promoter Region in the Hypothalamus of Dolang Sheep During Pubertal Initiation. DNA Cell Biol. (IF 3.1) Pub Date : 2023-02-21 Zhiyuan Sui,Zhishuai Zhang,Yongjie Zhang,Jihu Zhang,Qingjin Li,Feng Xing
Lin28B plays an important role in puberty initiation in sheep. This study aimed to discuss the correlation between different growth periods and the methylation status of cytosine-guanine dinucleotide (CpG) islands in the promoter region of the Lin28B gene in the Dolang sheep's hypothalamus. In this study, the sequence of the Lin28B gene promoter region in Dolang sheep was obtained by cloning and sequencing
-
Diverse Functions of MiR-425 in Human Cancer. DNA Cell Biol. (IF 3.1) Pub Date : 2023-02-16 Zhichao Wang,Wenjie Xie,Hongzai Guan
miRNAs are a type of small endogenous noncoding RNA composed of 20-22 nucleotides that can regulate gene expression by targeting the 3' untranslated region of mRNA. Many investigations have discovered that miRNAs have a role in the development and progression of human cancer. Several aspects of tumor development are affected by miR-425, including growth, apoptosis, invasion, migration, epithelial-mesenchymal
-
Diagnostics and Therapeutic Potential of miR-205 and miR-34a in Ovarian Cancer Management: A miRNA-Target-Based Analysis. DNA Cell Biol. (IF 3.1) Pub Date : 2023-02-13 Vivek Kumar,Archana Pandey,Arisha Arora,Priyanka Gautam,Deepa Bisht,Sameer Gupta,Amrita Chaurasia,Manisha Sachan
Epithelial ovarian cancer (EOC) treatment strategies mainly focused on surgery combined with chemotherapy. Recent targeted therapy techniques emerge as milestone and could be used for management of ovarian cancer (OC) progression with more efficacy. The aim is to evaluate the therapeutic and diagnostic potential of microRNA (miRNA) in management of EOC using in silico and quantitative real-time PCR