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Editorial evolution: welcoming our new editors-in-chief Curr. Genet. (IF 2.5) Pub Date : 2024-03-16 Michael Polymenis, Lydia Bogomolnaya, Jennifer Gallagher
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GATA family transcription factors in alga Chlamydomonas reinhardtii Curr. Genet. (IF 2.5) Pub Date : 2024-02-14 Pavel A. Virolainen, Elena M. Chekunova
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Ino2, activator of yeast phospholipid biosynthetic genes, interacts with basal transcription factors TFIIA and Bdf1 Curr. Genet. (IF 2.5) Pub Date : 2023-11-10 Maike Engelhardt, Stefan Hintze, Eva-Carina Wendegatz, Julia Lettow, Hans-Joachim Schüller
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Expression of human BRCA2 in Saccharomyces cerevisiae complements the loss of RAD52 in double-strand break repair Curr. Genet. (IF 2.5) Pub Date : 2023-11-07 Sherrice Law, Hannah Park, Eyar Shany, Sumer Sandhu, Mayukha Vallabhaneni, Damon Meyer
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Tips for efficiently maintaining pET expression plasmids Curr. Genet. (IF 2.5) Pub Date : 2023-11-08 Diana Khananisho, Alister J. Cumming, Daria Kulakova, Patrick J. Shilling, Daniel O. Daley
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Functional insights of three RING-finger peroxins in the life cycle of the insect pathogenic fungus Beauveria bassiana Curr. Genet. (IF 2.5) Pub Date : 2023-11-01 Jia-Hui Lei, Ting-Fei Sun, Ming-Guang Feng, Sheng-Hua Ying
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Comparative transcriptome analysis to unveil genes affecting the host cuticle destruction in Metarhizium rileyi Curr. Genet. (IF 2.5) Pub Date : 2023-09-19 Liqin Fan, Xinxin Li, Hongli Li, Bingjie Li, Jiahui Wang, Le He, Zhongkang Wang, Yunlong Lin
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TOF1 and RRM3 reveal a link between gene silencing and the pausing of replication forks Curr. Genet. (IF 2.5) Pub Date : 2023-06-22 Kholoud Shaban, Andrew Dolson, Ashley Fisher, Emma Lessard, Safia Mahabub Sauty, Krassimir Yankulov
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Yeast of Eden: microbial resistance to glyphosate from a yeast perspective Curr. Genet. (IF 2.5) Pub Date : 2023-06-03 Dionysios Patriarcheas, Taizina Momtareen, Jennifer E. G. Gallagher
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Insights from metagenome-assembled genomes on the genetic stability and safety of over-the-counter probiotic products Curr. Genet. (IF 2.5) Pub Date : 2023-05-26 Ghazal Aziz, Arsalan Zaidi, Daniel J. O.’ Sullivan
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Amyloids and prions in the light of evolution Curr. Genet. (IF 2.5) Pub Date : 2023-05-10 Alexey P. Galkin, Evgeniy I. Sysoev, Anna A. Valina
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The antifungal effect induced by itraconazole in Candida parapsilosis largely depends on the oxidative stress generated at the mitochondria Curr. Genet. (IF 2.5) Pub Date : 2023-04-29 Mª Luz Muñoz-Megías, Ruth Sánchez-Fresneda, Francisco Solano, Sergi Maicas, María Martínez-Esparza, Juan-Carlos Argüelles
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The N-terminal disordered region of ChsB regulates its efficient transport to the hyphal apical surface in Aspergillus nidulans Curr. Genet. (IF 2.5) Pub Date : 2023-04-18 Jingyun Jin, Ryo Iwama, Hiroyuki Horiuchi
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Tfs1, transcription elongation factor TFIIS, has an impact on chromosome segregation affected by pka1 deletion in Schizosaccharomyces pombe Curr. Genet. (IF 2.5) Pub Date : 2023-04-13 Kouhei Takenaka, Shiho Nishioka, Yuki Nishida, Makoto Kawamukai, Yasuhiro Matsuo
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Application of nanotags and nanobodies for live cell single-molecule imaging of the Z-ring in Escherichia coli Curr. Genet. (IF 2.5) Pub Date : 2023-04-06 Emma Westlund, Axel Bergenstråle, Alaska Pokhrel, Helena Chan, Ulf Skoglund, Daniel O. Daley, Bill Söderström
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Diverse and dynamic forms of gene regulation by the S. cerevisiae histone methyltransferase Set1 Curr. Genet. (IF 2.5) Pub Date : 2023-03-31 Neha Deshpande, Mary Bryk
Gene transcription is an essential and highly regulated process. In eukaryotic cells, the structural organization of nucleosomes with DNA wrapped around histone proteins impedes transcription. Chromatin remodelers, transcription factors, co-activators, and histone-modifying enzymes work together to make DNA accessible to RNA polymerase. Histone lysine methylation can positively or negatively regulate
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Morphogenic plasticity: the pathogenic attribute of Candida albicans Curr. Genet. (IF 2.5) Pub Date : 2023-03-22 Priya Prasad, Meena Tippana
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Genomic analyses of drug-resistant Salmonella enterica serovar Heidelberg strains isolated from meat and related sources between 2013 and 2017 in the south region of Brazil Curr. Genet. (IF 2.5) Pub Date : 2023-03-15 Felipe Pinheiro Vilela, Dália dos Prazeres Rodrigues, Marc William Allard, Juliana Pfrimer Falcão
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Functional characterization and comparative analysis of gene repression-mediating domains interacting with yeast pleiotropic corepressors Sin3, Cyc8 and Tup1 Curr. Genet. (IF 2.5) Pub Date : 2023-03-01 Julia Lettow, Felix Kliewe, Rasha Aref, Hans-Joachim Schüller
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Mitochondria in Cryptococcus: an update of mitochondrial transcriptional regulation in Cryptococcus Curr. Genet. (IF 2.5) Pub Date : 2023-02-02 Yang Meng, Chen Ding
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The essential liaison of two copper proteins: the Cu-sensing transcription factor Mac1 and the Cu/Zn superoxide dismutase Sod1 in Saccharomyces cerevisiae Curr. Genet. (IF 2.5) Pub Date : 2022-12-01 Dimitra Dialynaki, Athanasia Stavropoulou, Maria Laskou, Despina Alexandraki
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Horizontal transfer of tRNA genes to mitochondrial plasmids facilitates gene loss from fungal mitochondrial DNA Curr. Genet. (IF 2.5) Pub Date : 2022-11-30 Mathijs Nieuwenhuis, Jeroen Groeneveld, Duur K. Aanen
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Novel determinants of cell size homeostasis in the opportunistic yeast Candida albicans Curr. Genet. (IF 2.5) Pub Date : 2022-11-30 Julien Chaillot, Michael A. Cook, Adnane Sellam
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VdERG2 was involved in ergosterol biosynthesis, nutritional differentiation and virulence of Verticillium dahliae Curr. Genet. (IF 2.5) Pub Date : 2022-11-22 Junyuan Lv, Shichao Liu, Xiaojian Zhang, Lihong Zhao, Tao Zhang, Zhigang Zhang, Zili Feng, Feng Wei, Jinglong Zhou, Ruiyuan Zhao, Hongjie Feng, Heqin Zhu, Caihong Li, Yalin Zhang
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Nonribosomal peptide synthetase gene clusters and characteristics of predicted NRPS-dependent siderophore synthetases in Armillaria and other species in the Physalacriaceae Curr. Genet. (IF 2.5) Pub Date : 2022-11-11 Deborah L. Narh Mensah, Brenda D. Wingfield, Martin P. A. Coetzee
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Transcriptional noise adjusted for expression levels reveals genes with high transcriptional noise that are highly expressed, functionally related, and co-regulated in yeast Curr. Genet. (IF 2.5) Pub Date : 2022-10-17 Peter M. Palenchar, Thomas DeStefanis
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Peroxisome biogenesis and inter-organelle communication: an indispensable role for Pex11 and Pex30 family proteins in yeast Curr. Genet. (IF 2.5) Pub Date : 2022-10-15 Nayan Moni Deori, Shirisha Nagotu
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Synergistic roles of the phospholipase B homolog Plb1 and the cAMP-dependent protein kinase Pka1 in the hypertonic stress response of Schizosaccharomyces pombe Curr. Genet. (IF 2.5) Pub Date : 2022-09-16 Yasuhiro Matsuo, Stevan Marcus, Makoto Kawamukai
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The necrosis- and ethylene-inducing peptide 1-like protein (NLP) gene family of the plant pathogen Corynespora cassiicola Curr. Genet. (IF 2.5) Pub Date : 2022-09-13 Thaís Carolina da Silva Dal’Sasso, Vinícius Delgado da Rocha, Hugo Vianna Silva Rody, Maximiller Dal-Bianco Lamas Costa, Luiz Orlando de Oliveira
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Wide mutational analysis to ascertain the functional roles of eL33 in ribosome biogenesis and translation initiation Curr. Genet. (IF 2.5) Pub Date : 2022-08-22 Pilar Martín-Marcos, Álvaro Gil-Hernández, Mercedes Tamame
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A novel Zn2-Cys6 transcription factor clcA contributes to copper homeostasis in Aspergillus fumigatus Curr. Genet. (IF 2.5) Pub Date : 2022-08-16 Yoko Kusuya, Cai Bian, Daisuke Hagiwara, Sayaka Ban, Hiroki Takahashi
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Dysfunction of Ras-GAP protein AfgapA contributes to hypoxia fitness in Aspergillus fumigatus Curr. Genet. (IF 2.5) Pub Date : 2022-08-08 Cai Bian, Yoko Kusuya, Daisuke Hagiwara, Sayaka Ban, Yu Lu, Masaki Nagayama, Hiroki Takahashi
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Metagenomic views on taxonomic and functional profiles of the Himalayan Tsomgo cold lake and unveiling its deterzome potential Curr. Genet. (IF 2.5) Pub Date : 2022-08-05 Ashutosh Kumar Singh, Megha Kumari, Nitish Sharma, Amit Kumar Rai, Sudhir P. Singh
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Nab3 nuclear granule accumulation is driven by respiratory capacity Curr. Genet. (IF 2.5) Pub Date : 2022-08-03 Katherine M. Hutchinson, Jeremy C. Hunn, Daniel Reines
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Insertion orientation within the cassette affects gene-targeting success during ends-out recombination in the yeast Saccharomyces cerevisiae Curr. Genet. (IF 2.5) Pub Date : 2022-07-06 Petar Tomev Mitrikeski
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The vacuolar morphology protein VAC14 plays an important role in sexual development in the filamentous ascomycete Sordaria macrospora Curr. Genet. (IF 2.5) Pub Date : 2022-07-01 Anika Groth, Svenja Ahlmann, Antonia Werner, Stefanie Pöggeler
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Whole genome sequencing and comparative genomic analyses of Pseudomonas aeruginosa strain isolated from arable soil reveal novel insights into heavy metal resistance and codon biology Curr. Genet. (IF 2.5) Pub Date : 2022-06-28 Jayanti Saha, Sourav Dey, Ayon Pal
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Synthetic negative genome screen of the GPN-loop GTPase NPA3 in Saccharomyces cerevisiae Curr. Genet. (IF 2.5) Pub Date : 2022-06-04 Martín Mora-García, Diana Ascencio, Tania Félix-Pérez, Judith Ulloa-Calzonzin, Alejandro Juárez-Reyes, Karina Robledo-Márquez, Yolanda Rebolloso-Gómez, Lina Riego-Ruiz, Alexander DeLuna, Mónica R. Calera, Roberto Sánchez-Olea
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Suggestion for a new bacteriophage genus for the Klebsiella pneumoniae phage vB_KpnS-Carvaje Curr. Genet. (IF 2.5) Pub Date : 2022-06-06 Jéssica C. Sousa, Sanna Sillankorva, Alberta Faustino, Carla M. Carvalho
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Genomic insights into the diversity of non-coding RNAs in Bacillus cereus sensu lato Curr. Genet. (IF 2.5) Pub Date : 2022-05-12 Kátia B. Gonçalves, Renan J. Casarotto Appel, Laurival A. Vilas Bôas, Priscilla F. Cardoso, Gislayne T. Vilas Bôas
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Cold-adaptive traits identified by comparative genomic analysis of a lipase-producing Pseudomonas sp. HS6 isolated from snow-covered soil of Sikkim Himalaya and molecular simulation of lipase for wide substrate specificity Curr. Genet. (IF 2.5) Pub Date : 2022-05-09 Loreni Chiring Phukon, Rounak Chourasia, Srichandan Padhi, Md Minhajul Abedin, Tharangattumana Krishnan Godan, Binod Parameswaran, Sudhir P. Singh, Amit Kumar Rai
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New destination vectors facilitate Modular Cloning for Chlamydomonas Curr. Genet. (IF 2.5) Pub Date : 2022-04-16 Justus Niemeyer, Michael Schroda
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Deciphering β-tubulin gene of carbendazim resistant Fusarium solani isolate and its comparison with other Fusarium species Curr. Genet. (IF 2.5) Pub Date : 2022-04-13 Mrinmay Tarafder, Bejoysekhar Datta
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How adaptive laboratory evolution can boost yeast tolerance to lignocellulosic hydrolyses Curr. Genet. (IF 2.5) Pub Date : 2022-04-01 Yasmine Alves Menegon, Jeferson Gross, Ana Paula Jacobus
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Molecular basis of cycloheximide resistance in the Ophiostomatales revealed Curr. Genet. (IF 2.5) Pub Date : 2022-03-22 Brenda D. Wingfield, Mike J. Wingfield, Tuan A. Duong
Resistance to the antibiotic Cycloheximide has been reported for a number of fungal taxa. In particular, some yeasts are known to be highly resistant to this antibiotic. Early research showed that this resulted from a transition mutation in one of the 60S ribosomal protein genes. In addition to the yeasts, most genera and species in the Ophiostomatales are highly resistant to this antibiotic, which
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Variable penetrance of Nab3 granule accumulation quantified by a new tool for high-throughput single-cell granule analysis Curr. Genet. (IF 2.5) Pub Date : 2022-03-17 Jeremy C. Hunn, Katherine M. Hutchinson, Joshua B. Kelley, Daniel Reines
Reorganization of cellular proteins into subcellular compartments, such as the concentration of RNA-binding proteins into cytoplasmic stress granules and P-bodies, is a well-recognized, widely studied physiological process currently under intense investigation. One example of this is the induction of the yeast Nab3 transcription termination factor to rearrange from its pan-nucleoplasmic distribution
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Application of recyclable CRISPR/Cas9 tools for targeted genome editing in the postharvest pathogenic fungi Penicillium digitatum and Penicillium expansum Curr. Genet. (IF 2.5) Pub Date : 2022-03-17 Sandra Garrigues, Paloma Manzanares, Jose F. Marcos
Penicillium digitatum and Penicillium expansum are plant pathogenic fungi that cause the green and blue mold diseases, respectively, leading to serious postharvest economic losses worldwide. Moreover, P. expansum can produce mycotoxins, which are hazardous compounds to human and animal health. The development of tools that allow multiple and precise genetic manipulation of these species is crucial
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TAL effectors and the predicted host targets of pomegranate bacterial blight pathogen Xanthomonas citri pv. punicae Curr. Genet. (IF 2.5) Pub Date : 2022-03-11 Sruthi Sivaraman, Dineshkumar Krishnamoorthy, Kumar Arvind, Tony Grace, Jyotsana Sharma, Ginny Antony
The molecular mechanism of pomegranate susceptibility to bacterial blight, a serious threat to pomegranate production in India, is largely unknown. In the current study, we have used PacBio and Illumina sequencing of Xanthomonas citri pv. punicae (Xcp) strain 119 genome to identify tal genes and RNA-Seq analysis to identify putative host targets in the susceptible pomegranate variety Bhagwa challenged
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Increased peroxisome proliferation is associated with early yeast replicative ageing Curr. Genet. (IF 2.5) Pub Date : 2022-02-27 Rachayeeta Deb, Suchetana Ghose, Shirisha Nagotu
Peroxisomes are single membrane-bound organelles ubiquitously present in several cell types and are associated with cell and tissue-specific functions. Their role in cellular ageing is under investigation in various model systems. Metabolism of cellular reactive oxygen species is a universal function performed by these organelles. In this study, we investigated alterations in peroxisome number upon
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Yeast Stn1 promotes MCM to circumvent Rad53 control of the S phase checkpoint Curr. Genet. (IF 2.5) Pub Date : 2022-02-12 Hovik Gasparayan, Chris Caridi, Jeff Julius, Wenyi Feng, Jeff Bachant, Constance I. Nugent
Treating yeast cells with the replication inhibitor hydroxyurea activates the S phase checkpoint kinase Rad53, eliciting responses that block DNA replication origin firing, stabilize replication forks, and prevent premature extension of the mitotic spindle. We previously found overproduction of Stn1, a subunit of the telomere-binding Cdc13–Stn1–Ten1 complex, circumvents Rad53 checkpoint functions in
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The yeast Dbf4 Zn2+ finger domain suppresses single-stranded DNA at replication forks initiated from a subset of origins Curr. Genet. (IF 2.5) Pub Date : 2022-02-11 Jeff Bachant, Elizabeth A. Hoffman, Chris Caridi, Constance I. Nugent, Wenyi Feng
Dbf4 is the cyclin-like subunit for the Dbf4-dependent protein kinase (DDK), required for activating the replicative helicase at DNA replication origin that fire during S phase. Dbf4 also functions as an adaptor, targeting the DDK to different groups of origins and substrates. Here we report a genome-wide analysis of origin firing in a budding yeast mutant, dbf4-zn, lacking the Zn2+ finger domain within
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Investigating cell autonomy in microorganisms Curr. Genet. (IF 2.5) Pub Date : 2022-02-04 Sarah Piccirillo, Andrew P. Morgan, Andy Y. Leon, Annika L. Smith, Saul M. Honigberg
Cell–cell signaling in microorganisms is still poorly characterized. In this Methods paper, we describe a genetic procedure for detecting cell-nonautonomous genetic effects, and in particular cell–cell signaling, termed the chimeric colony assay (CCA). The CCA measures the effect of a gene on a biological response in a neighboring cell. This assay can measure cell autonomy for range of biological activities
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Genome-wide analysis reveals a rhamnolipid-dependent modulation of flagellar genes in Pseudomonas aeruginosa PAO1 Curr. Genet. (IF 2.5) Pub Date : 2022-01-30 Michele R. Castro, Graciela M. Dias, Tiago S. Salles, Nubia M. Cabral, Danielly C. O. Mariano, Hadassa L. Oliveira, Eliana S. F. W. Abdelhay, Renata Binato, Bianca C. Neves
Pseudomonas aeruginosa is an opportunistic pathogen and an important model organism for the study of bacterial group behaviors, including cell motility and biofilm formation. Rhamnolipids play a pivotal role in biofilm formation and motility phenotypes in P. aeruginosa, possibly acting as wetting agents and mediating chemotactic stimuli. However, no biochemical mechanism or gene regulatory network
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Prophage-encoded gene VpaChn25_0734 amplifies ecological persistence of Vibrio parahaemolyticus CHN25 Curr. Genet. (IF 2.5) Pub Date : 2022-01-22 Xu, Yingwei, Yang, Lianzhi, Wang, Yaping, Zhu, Zhuoying, Yan, Jizhou, Qin, Si, Chen, Lanming
Vibrio parahaemolyticus is a waterborne pathogen that can cause acute gastroenteritis, wound infection, and septicemia in humans. The molecular basis of its pathogenicity is not yet fully understood. Phages are found most abundantly in aquatic environments and play a critical role in horizontal gene transfer. Nevertheless, current literature on biological roles of prophage-encoded genes remaining in
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Development of versatile and efficient genetic tools for the marine-derived fungus Aspergillus terreus RA2905 Curr. Genet. (IF 2.5) Pub Date : 2022-01-19 Yao, Guangshan, Chen, Xiaofeng, Han, Yijuan, Zheng, Huawei, Wang, Zonghua, Chen, Jianming
Marine-derived Aspergillus terreus produces a variety of structurally novel secondary metabolites, most of which show unique biological activities. However, the lack of efficient genetic tools limits the discovery of new compounds, the elucidation of involved biosynthesis mechanism, as well as the strain engineering efforts. Therefore, in this study, we first established both an effective PEG-mediated
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A positive genetic selection for transmembrane domain mutations in HRD1 underscores the importance of Hrd1 complex integrity during ERAD Curr. Genet. (IF 2.5) Pub Date : 2022-01-18 Nakatsukasa, Kunio, Wigge, Sylvia, Takano, Yuki, Kawarasaki, Tomoyuki, Kamura, Takumi, Brodsky, Jeffrey L.
Misfolded proteins in the endoplasmic reticulum (ER) are retrotranslocated to the cytosol for ubiquitination and degradation by the proteasome. During this process, known as ER-associated degradation (ERAD), the ER-embedded Hrd1 ubiquitin ligase plays a central role in recognizing, ubiquitinating, and retrotranslocating scores of lumenal and integral membrane proteins. To better define the mechanisms
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Histone 3 lysine 4 monomethylation supports activation of transcription in S. cerevisiae during nutrient stress Curr. Genet. (IF 2.5) Pub Date : 2022-01-18 Deshpande, Neha, Jordan, Rachel, Henderson Pozzi, Michelle, Bryk, Mary
Mono-methylation of the fourth lysine on the N-terminal tail of histone H3 was found to support the induction of RNA polymerase II transcription in S. cerevisiae during nutrient stress. In S. cerevisiae, the mono-, di- and tri-methylation of lysine 4 on histone H3 (H3K4) is catalyzed by the protein methyltransferase, Set1. The three distinct methyl marks on H3K4 act in discrete ways to regulate transcription
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Emerging roles of SWI/SNF remodelers in fungal pathogens Curr. Genet. (IF 2.5) Pub Date : 2022-01-10 Balachandra, Vinutha K., Ghosh, Santanu K.
Fungal pathogens constantly sense and respond to the environment they inhabit, and this interaction is vital for their survival inside hosts and exhibiting pathogenic traits. Since such responses often entail specific patterns of gene expression, regulators of chromatin structure contribute to the fitness and virulence of the pathogens by modulating DNA accessibility to the transcriptional machinery
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Molecular characterization of lipase from a psychrotrophic bacterium Pseudomonas sp. CRBC14 Curr. Genet. (IF 2.5) Pub Date : 2021-11-27 Farooq, Saleem, Ganai, Shabir Ahmad, Ganai, Bashir Ahmad, Mohan, Suma, Uqab, Baba, Nazir, Ruqeya
Lipases from Pseudomonas species are particularly useful due to their broader biocatalytic applications and temperature activity. In this study, we amplified the gene encoding wild-type cold-active lipase from the genome of psychrotrophic bacterium isolated from the Himalayan glacier. The isolated CRBC14 strain was identified as Pseudomonas sp. based on the 16S rRNA gene sequence. Lipase activity was
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Beyond the canonical role of TFIIB in eukaryotic transcription Curr. Genet. (IF 2.5) Pub Date : 2021-11-19 O’Brien, Michael J., Ansari, Athar
The role of general transcription factor TFIIB in transcription extends well beyond its evolutionarily conserved function in initiation. Chromatin localization studies demonstrating binding of TFIIB to both the 5’ and 3’ ends of genes in a diverse set of eukaryotes strongly suggested a rather unexpected role of the factor in termination. TFIIB indeed plays a role in termination of transcription. TFIIB