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Effects of aflatoxin and fumonisin on gene expression of growth factors and inflammation-related genes in a human hepatocyte cell line Mutagenesis (IF 2.7) Pub Date : 2024-03-12 Hang Wu, Ya Xu, Yun Yun Gong, John Huntriss, Michael N Routledge
Aflatoxin B1 (AFB1) and fumonisin B1 (FB1) are mycotoxins widely distributed in maize and maized-based products, often occurring together. The implications of co-exposure to aflatoxin and fumonsin for human health are numerous, but a particular concern is the potential of FB1 to modulate AFB1 hepatotoxicity. This study evaluated the toxicity of these mycotoxins, alone or combined, in a human non-tumorigenic
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Inflammation and Gut Barrier Function-Related Genes and Colorectal Cancer Risk in Western European Populations Mutagenesis (IF 2.7) Pub Date : 2024-03-05 Hannah B Mandle, Mazda Jenab, Marc J Gunter, Anne Tjønneland, Anja Olsen, Christina C Dahm, Jie Zhang, Pierre-Emmanuel Sugier, Joseph Rothwell, Gianluca Severi, Rudolf Kaaks, Verena A Katzke, Matthias B Schulze, Giovanna Masala, Sabina Sieri, Salvatore Panico, Carlotta Sacerdote, Catalina Bonet, Maria-Jose Sánchez, Pilar Amiano, José María Huerta, Marcela Guevara, Richard Palmqvist, Thyra Löwenmark
Gut barrier dysfunction and related inflammation are known to be associated with the development and progression of colorectal cancer (CRC). We investigated associations of 292 single-nucleotide polymorphisms (SNPs) from 27 genes related to endotoxins/lipopolysaccharide (LPS) sensing and tolerance, mucin synthesis, inflammation, and Crohn’s disease with colon and rectal cancer risks. Incident CRC cases
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Genetic And Environmental Associations Of Nonspecific Chromosomal Aberrations Mutagenesis (IF 2.7) Pub Date : 2024-02-28 Kari Hemminki, Yasmeen Niazi, Ludmila Vodickova, Pavel Vodicka, Asta Försti
Nonspecific structural chromosomal aberrations (CAs) are found in around 1% of circulating lymphocytes from healthy individuals but the frequency may be higher after exposure to carcinogenic chemicals or radiation. CAs have been used in the monitoring of persons exposed to genotoxic agents and radiation. Previous studies on occupationally exposed individuals have shown associations between the frequency
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Next-Generation Therapeutics for Rare Genetic Disorders Mutagenesis (IF 2.7) Pub Date : 2024-02-09 Akhila Sankar, Ravi Kumar Y.S., Anjali Singh, Riya Roy, Rashmi Shukla, Bhupendra Verma
The therapeutic potential of the human genome has been explored through the development of next-generation therapeutics, which have had a high impact on treating genetic disorders. Classical treatments have traditionally focused on common diseases that require repeated treatments. However, with the recent advancements in the development of nucleic acids, utilizing DNA and RNA to modify or correct gene
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An integrated in vitro carcinogenicity test that distinguishes between genotoxic carcinogens, non-genotoxic carcinogens, and non-carcinogens Mutagenesis (IF 2.7) Pub Date : 2024-02-01 Katherine E Chapman, Ume-Kulsoom Shah, Jessica F Fletcher, George E Johnson, Shareen H Doak, Gareth J S Jenkins
Chemical safety testing plays a crucial role in product and pharmacological development, as well as chemoprevention; however, in vitro genotoxicity safety tests do not always accurately predict the chemicals that will be in vivo carcinogens. If chemicals test positive in vitro for genotoxicity but negative in vivo, this can contribute to unnecessary testing in animals used to confirm erroneous in vitro
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Measurement of chromosomal instability and level of DNA damage in peripheral blood mononuclear cells of endometrial cancer patients Mutagenesis (IF 2.7) Pub Date : 2024-01-25 Aleksandra Marković, Darko Grujičić, Marija Živković Radojević, Olivera Milošević-Djordjević
Endometrial cancer is one of the most common invasive gynecologic malignancies in developed countries. The aim of this study was to evaluate chromosomal instability and level of DNA damage in peripheral blood mononuclear cells (PBMCs) of newly diagnosed endometrial cancer patients in relation to health status (diagnosis), age, histological grade of cancer, residence, smoking, number of pregnancies
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N-Nitrosodimethylamine investigations in Muta™Mouse define point-of-departure values and demonstrate less-than-additive somatic mutant frequency accumulations Mutagenesis (IF 2.7) Pub Date : 2024-01-06 Anthony M Lynch, Jonathan Howe, Deon Hildebrand, James S Harvey, Mark Burman, Danielle S G Harte, Liangfu Chen, Casey Kmett, Wei Shi, Charles McHugh, Kinnari Patel, Venkat Junnotula, Julia Kenny, Richard Howarth, John W Wills
The N-nitrosamine, N-nitrosodimethylamine (NDMA), is an environmental mutagen and rodent carcinogen. Small levels of NDMA have been identified as an impurity in some commonly used drugs, resulting in several product recalls. In this study, NDMA was evaluated in an OECD TG-488 compliant Muta™Mouse gene mutation assay (28-day oral dosing across 7 daily doses of 0.02-4 mg/kg/day) using an integrated design
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Assessment of the three-test genetic toxicology battery for groundwater metabolites Mutagenesis (IF 2.7) Pub Date : 2024-01-06 Paul Fowler, Alessandra Bearzatto, Carol Beevers, Ewan Booth, E Maria Donner, Lin Gan, Kerstin Hartmann, Krista Meurer, Maaike E Schutte, Raja S Settivari
The two-test in vitro battery for genotoxicity testing (Ames and micronucleus) has, in the majority of cases replaced the three-test battery (as two test plus mammalian cell gene mutation assay) for the routine testing of chemicals, pharmaceuticals, cosmetics and agrochemical metabolites originating from food and feed as well as from water treatment. The guidance for testing of agrochemical groundwater
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Expression and clinical diagnostic value of CCHE1 in breast cancer Mutagenesis (IF 2.7) Pub Date : 2023-12-27 Feng Zhao, Wenjuan Zhou, Ran Xin, Xin Miao
Objective Breast cancer is a malignant tumor in the epithelial tissue of the breast gland. This study aimed to unveil the expression and clinical diagnostic value of lncRNA cervical cancer high-expressed 1 (CCHE1) in breast cancer. Methods CCHE1 expression in breast cancer tissues was evaluated by RT-qPCR. The relationship between the CCHE1 expression and clinicopathological features of breast cancer
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Ames Test study designs for nitrosamine mutagenicity testing: qualitative and quantitative analysis of key assay parameters Mutagenesis (IF 2.7) Pub Date : 2023-12-19 Dean N Thomas, John W Wills, Helen Tracey, Sandy J Baldwin, Mark Burman, Abbie N Williams, Danielle S G Harte, Ruby A Buckley, Anthony M Lynch
The robust control of genotoxic N-nitrosamine (NA) impurities is an important safety consideration for the pharmaceutical industry, especially considering recent drug product withdrawals. NAs belong to the “Cohort of Concern” list of genotoxic impurities (ICH M7) because of the mutagenic and carcinogenic potency of this chemical class. In addition, regulatory concerns exist regarding the capacity of
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Inhibition of homologous recombination repair by Mirin in ovarian cancer ameliorates carboplatin therapy response in vitro Mutagenesis (IF 2.7) Pub Date : 2023-12-14 Josef Horak, Dominika Vallusova, Andrea Cumova, Petr Holy, Pavel Vodicka, Alena Opattova
Chemoresistance poses one of the most significant challenges of cancer therapy. Carboplatin (CbPt) is one of the most used chemotherapeutics in ovarian cancer (OVC) treatment. MRE11 constitutes a part of homologous recombination (HR), which is responsible for the repair of CbPt-induced DNA damage, particularly DNA crosslinks. The study’s main aim was to address the role of HR in CbPt chemoresistance
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Maternal exercise during pregnancy modulates genotoxicity caused by high fructose consumption in mice offspring Mutagenesis (IF 2.7) Pub Date : 2023-11-30 Marina Lummertz Magenis, Adriani Paganini Damiani, Isadora de Oliveira Monteiro, Ligia Salvan Dagostin, Nicollas dos Santos da Silva, Rahisa Scussel, Seigo Nagashima, Sabine A S Langie, Ricardo Aurino Pinho, Vanessa Moraes de Andrade
Pregnancy is a period that is characterized by several metabolic and physiological changes and requires special attention, especially with regard to the relationship between feeding and fetal development. Therefore, the objective of this study was to evaluate whether the practice of voluntary physical exercise in combination with chronic consumption of fructose from the beginning of life and/or until
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Long or short? Telomere length and pancreatic cancer and its precursor lesions, a narrative review Mutagenesis (IF 2.7) Pub Date : 2023-11-16 Daniele Campa, Alessio Felici, Chiara Corradi, Giulia Peduzzi, Manuel Gentiluomo, Riccardo Farinella, Cosmeri Rizzato
Pancreatic ductal adenocarcinoma (PDAC) is the most common and lethal form of pancreatic cancer, with a survival approaching only 11% at five years after diagnosis. In the last 15 years, telomere length measured in leukocyte (LTL) has been studied in relation to PDAC risk. The majority of the studies reported an association between short LTL and increased PDAC risk, but the results are heterogeneous
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Matrine and Oxymatrine: Evaluating the gene mutation potential using in silico tools and the bacterial reverse mutation assay (Ames test). Mutagenesis (IF 2.7) Pub Date : 2023-10-25 Benjamin Christian Fischer,Yemurai Musengi,Jeannette König,Benjamin Sachse,Stefanie Hessel-Pras,Bernd Schäfer,Carsten Kneuer,Kristin Herrmann
The quinolizidine alkaloids matrine and its N-oxide oxymatrine occur in plants of the genus Sophora. Recently, matrine was sporadically detected in liquorice products. Morphological similarity of the liquorice plant Glycyrrhiza glabra with Sophora species and resulting confusion during harvesting may explain this contamination, but use of matrine as pesticide has also been reported. The detection of
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Obituary to John Ashby (19 April 1943-03 October 2022). Mutagenesis (IF 2.7) Pub Date : 2023-10-14 David Kirkland,George Douglas
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The relationship between lymphocyte DNA damage and coronary artery disease, blood trace elements Mutagenesis (IF 2.7) Pub Date : 2023-10-11 İsmail Erdoğu, Emrah Dural, Hüseyin Altundağ, Tülay Oymak, Halit Sinan Süzen
Somatic DNA damage and causative factors (occupational exposures, foods and habits etc.) are thought to contribute to the pathogenesis of atherosclerosis, although knowledge about their role in coronary artery disease is still insufficient. This study aimed to determine the effects of lymphocyte-DNA damage and blood-trace element concentrations on coronary artery disease (CAD). The single-cell alkaline
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The known unknowns of mitochondrial carcinogenesis: de novo NUMTs and intercellular mitochondrial transfer Mutagenesis (IF 2.7) Pub Date : 2023-10-07 Tigran Harutyunyan
The translocation of mitochondrial DNA (mtDNA) sequences into the nuclear genome, resulted in the occurrence of nuclear sequences of mitochondrial origin (NUMTs) which can be detected in nearly all sequenced eukaryotes. However, de novo mtDNA insertions can contribute to the development of pathological conditions including cancer. Recent data indicate that de novo mtDNA translocation into chromosomes
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In vitro chemopreventive and cytotoxic effects of Amazon mosses Leucobryum martianum (Hornsch.) and Leucobryum laevifolium (Broth) extracts Mutagenesis (IF 2.7) Pub Date : 2023-09-30 Andreia da Silva Fernandes, Carine Gonçalves de Oliveira, Heitor Evangelista, Maria Sulamita DS, Carlos Fernando Araujo-Lima, Israel Felzenszwalb
Several bioactive compounds, such as polyphenols, demonstrate low toxicity and prominent effects on cancer cells with antioxidant, anti-inflammatory, and antitumor activities. Such compounds can be found in Amazon mosses Leucobryum martianum (Hornsch.) Hampe ex Müll. Hal. (Hornsch.) and Leucobryum laevifolium (Broth). Antimutagenic assay with Salmonella enterica serovar Typhimurium and cytotoxicity
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Effect of Iron and Calcium on radiation sensitivity in Prostate Cancer patients relative to controls Mutagenesis (IF 2.7) Pub Date : 2023-09-29 Varinderpal S Dhillon, Permal Deo, Michael Fenech
High intake of red meat and/or dairy products may increase the concentration of iron and calcium in plasma - a risk factor for prostate cancer (PC). Despite our understandings of nutrients and their effects on the genome, studies on the effects of iron and calcium on radiation sensitivity of PC patients are lacking. Therefore, we tested the hypothesis that high plasma levels of iron and calcium could
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TNS1 regulated by HLF represses the progression of prostate cancer Mutagenesis (IF 2.7) Pub Date : 2023-09-14 Hao Zhou, Fang Wang
Hepatic leukemia factor (HLF), a transcription factor, is dysregulated in many cancers. This study investigates the function of HLF in prostate cancer (PCa) and its relation to tensin 1 (TNS1). Clinical tissues were collected from 24 PCa patients. DU145 and PC3 cells overexpressing HLF were established. HLF signaling was downregulated in PCa tissues compared to adjacent tissues and in DU145 and PC3
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Multi-endpoint analysis of Cadmium Chloride induced genotoxicity shows role for reactive oxygen species and p53 activation in DNA damage induction, cell cycle irregularities and cell size aberrations Mutagenesis (IF 2.7) Pub Date : 2023-08-09 Leanne Stannard, Ann Doherty, Kate Chapman, Shareen Doak, Gareth Jenkins
Cadmium chloride (CdCl2) is a known genotoxic carcinogen, with a mechanism of action thought to partly involve the generation of reactive oxygen species (ROS). We applied here a multi-endpoint approach in vitro to explore the impact of CdCl2 on both the genome and on wider cell biology pathways relevant to cancer. Multi-endpoint approaches are believed to offer greater promise in terms of understanding
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Action-at-a-distance mutations induced by 8-oxo-7,8-dihydroguanine is dependent on APOBEC3 Mutagenesis (IF 2.7) Pub Date : 2023-07-20 Ruriko Fukushima, Tetsuya Suzuki, Akari Kobayakawa, Hiroyuki Kamiya
DNA oxidation is a serious threat to genome integrity and involved in mutations and cancer initiation. The G base is most frequently damaged, and 8-oxo-7,8-dihydroguanine (G O, 8-hydroxyguanine) is one of the predominant damaged bases. In human cells, G O causes a G:C➔T:A transversion mutation at the modified site, and also induces untargeted substitution mutations at the G bases of 5'-GpA-3' dinucleotides
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Glutathione-Related Antioxidant Defence, DNA Damage and DNA Repair in Patients Suffering from Post-COVID Conditions Mutagenesis (IF 2.7) Pub Date : 2023-07-09 Selin Kankaya, Fatih Yavuz, Alper Tari, Ahmet Bera Aygün, Esra Gizem Güneş, Bahar Bektan Kanat, Gulru Ulugerger Avci, Hakan Yavuzer, Yıldız Dinçer
Post-COVID conditions are defined as the continuation of the symptoms of COVID-19 (Coronavirus Disease 2019) 3 months after the initial SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) infection, with no other explanation. Post-COVID conditions are seen among 30–60% of patients with asymptomatic or mild forms of COVID-19. The underlying pathophysiological mechanisms of post-COVID conditions
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Impact of Mobile Phone Specific Electromagnetic Fields on DNA Damage Caused by Occupationally Relevant Exposures: Results of ex vivo Experiments with Peripheral Blood Mononuclear Cells from Different Demographic Groups Mutagenesis (IF 2.7) Pub Date : 2023-07-05 Miroslav Mišík, Michael Kundi, Nadine Worel, Franziska Ferk, Hans-Peter Hutter, Michael Grusch, Armen Nersesyan, Denise Herrera Morales, Siegfried Knasmueller
Aim of this study was to investigate if age and body mass of humans have an impact on the DNA damaging properties of high-frequency mobile phone-specific electromagnetic fields (HF-EMF, 1950 MHz, UMTS signal) and if this form of radiation has an impact on the genotoxic effects of occupationally relevant exposures. Pooled peripheral blood mononuclear cells (PBMC) from three groups (young normal weight
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DNA strand break levels in cryopreserved mononuclear blood cell lines measured by the alkaline comet assay: results from the hCOMET ring trial Mutagenesis (IF 2.7) Pub Date : 2023-06-26 Peter Møller, Amaya Azqueta, Adriana Rodriguez-Garraus, Tamara Bakuradze, Elke Richling, Ezgi Eyluel Bankoglu, Helga Stopper, Victoria Claudino Bastos, Sabine A S Langie, Annie Jensen, Sara Ristori, Francesca Scavone, Lisa Giovannelli, Maria Wojewódzka, Marcin Kruszewski, Vanessa Valdiglesias, Blanca Laffon, Carla Costa, Solange Costa, João Paulo Teixeira, Mirko Marino, Cristian Del Bo’, Patrizia Riso
The comet assay is widely used in biomonitoring studies for the analysis of DNA damage in leukocytes and peripheral blood mononuclear cells. Rather than processing blood samples directly, it can be desirable to cryopreserve whole blood or isolated cells for later analysis by the comet assay. However, this creates concern about artificial accumulation of DNA damage during cryopreservation. In this study
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Long-term cryopreservation of potassium bromate positive assay controls for measurement of oxidatively damaged DNA by the Fpg-modified comet assay: results from the hCOMET ring trial Mutagenesis (IF 2.7) Pub Date : 2023-06-24 Peter Møller, Amaya Azqueta, Adriana Rodriguez-Garraus, Tamara Bakuradze, Elke Richling, Ezgi Eyluel Bankoglu, Helga Stopper, Victoria Claudino Bastos, Sabine A S Langie, Annie Jensen, Sara Ristori, Francesca Scavone, Lisa Giovannelli, Maria Wojewódzka, Marcin Kruszewski, Vanessa Valdiglesias, Blanca Laffon, Carla Costa, Solange Costa, João Paulo Teixeira, Mirko Marino, Cristian Del Bo’, Patrizia Riso
The formamidopyrimidine DNA glycosylase (Fpg)-modified comet assay is widely used for the measurement of oxidatively generated damage to DNA. However, there has not been a recommended long-term positive control for this version of the comet assay. We have investigated potassium bromate as a positive control for the Fpg-modified comet assay because it generates many Fpg-sensitive sites with little concurrent
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Genotoxicity of cytokines at chemotherapy-induced ‘storm’ concentrations in a model of the human bone marrow Mutagenesis (IF 2.7) Pub Date : 2023-06-16 Harshini S H Asurappulige, Adam D Thomas, H Ruth Morse
Donor cell leukaemia (DCL) is a complication of haematopoietic stem cell transplantation where donated cells become malignant within the patient’s bone marrow. As DCL predominates as acute myeloid leukaemia, we hypothesised that the cytokine storm following chemotherapy played a role in promoting and supporting leukaemogenesis. Cytokines have also been implicated in genotoxicity, thus we explored a
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Next Generation Sequencing Workshop at the Royal Society of Medicine (London, May 2022): How genomics is on the path to modernizing Genetic Toxicology Mutagenesis (IF 2.7) Pub Date : 2023-06-10 Anthony M Lynch, Thalita B Zanoni, Jesse J Salk, Inigo Martincorena, Robert R Young, Jill Kucab, Charles C Valentine, Carole Yauk, Patricia A Escobar, Kristine L Witt, Roland Frötschl, Simon H Reed, Anne Ashford
The use of error-corrected Next Generation Sequencing (ecNG) to determine mutagenicity has been a subject of growing interest and potentially a disruptive technology that could supplement, and in time, replace current testing paradigms in preclinical safety assessment. Considering this, a Next Generation Sequencing Workshop was held at the Royal Society of Medicine in London in May 2022, supported
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Current status and future challenges of genotoxicity OECD test guidelines (TGs) for nanomaterials: a workshop report Mutagenesis (IF 2.7) Pub Date : 2023-05-26 Shareen H Doak, Cristina Andreoli, Michael J Burgum, Qasim Chaudhry, Eric A J Bleeker, Cecilia Bossa, Josefa Domenech, Damjana Drobne, Valerie Fessard, Nina Jeliazkova, Eleonora Longhin, Elise Rundén-Pran, Maciej Stepnik, Naouale El Yamani, Julia Catalán, Maria Dusinska
Genotoxicity testing for nanomaterials remains challenging as standard testing approaches require some adaptation, and further development of nano-specific OECD Test Guidelines (TGs) and Guidance Documents (GDs) are needed. However, the field of genotoxicology continues to progress and new approach methodologies (NAMs) are being developed that could provide relevant information on the range of mechanisms
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Visual comet scoring revisited: a guide to scoring comet assay slides and obtaining reliable results Mutagenesis (IF 2.7) Pub Date : 2023-05-26 Peter Møller, Amaya Azqueta, Julen Sanz-Serrano, Tamara Bakuradze, Elke Richling, Ezgi Eyluel Bankoglu, Helga Stopper, Victoria Claudino Bastos, Sabine A S Langie, Annie Jensen, Francesca Scavone, Lisa Giovannelli, Maria Wojewódzka, Marcin Kruszewski, Vanessa Valdiglesias, Blanca Laffon, Carla Trindade Costa, Solange Costa, João Paulo Teixeira, Mirko Marino, Cristian Del Bo’, Patrizia Riso, Congying
Measurement of DNA migration in the comet assay can be done by image analysis or visual scoring. The latter accounts for 20-25% of the published comet assay results. Here we assess the intra- and inter-investigator variability in visual scoring of comets. We include three training sets of comet images, which can be used as reference for researchers who wish to use visual scoring of comets. Investigators
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Genotoxicity by rapeseed methyl ester and hydrogenated vegetable oil combustion exhaust products in lung epithelial (A549) cells Mutagenesis (IF 2.7) Pub Date : 2023-05-26 Monika Hezareh Rothmann, Peter Møller, Yona J Essig, Louise Gren, Vilhelm B Malmborg, Martin Tunér, Joakim Pagels, Annette M Krais, Martin Roursgaard
Biofuel is an attractive substitute for petrodiesel because of its lower environmental footprint. For instance, the polycyclic aromatic hydrocarbons (PAH) emission per fuel energy content is lower for rapeseed methyl ester (RME) than for petrodiesel. The present study assesses genotoxicity by extractable organic matter (EOM) of exhaust particles from combustion of petrodiesel, RME and hydrogenated
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Inter-laboratory variation in measurement of DNA damage by the alkaline comet assay in the hCOMET ring trial Mutagenesis (IF 2.7) Pub Date : 2023-05-25 Peter Møller, Amaya Azqueta, Miguel Collia, Tamara Bakuradze, Elke Richling, Ezgi Eyluel Bankoglu, Helga Stopper, Victoria Claudino Bastos, Sabine A S Langie, Annie Jensen, Sara Ristori, Francesca Scavone, Lisa Giovannelli, Maria Wojewódzka, Marcin Kruszewski, Vanessa Valdiglesias, Blanca Laffon, Carla Costa, Solange Costa, João Paulo Teixeira, Mirko Marino, Cristian Del Bo’, Patrizia Riso, Congying
The comet assay is a simple and versatile method for measurement of DNA damage in eukaryotic cells. More specifically, the assay detects DNA migration from agarose gel-embedded nucleoids, which depends on assay conditions and the level of DNA damage. Certain steps in the comet assay procedure have substantial impact on the magnitude of DNA migration (e.g. electric potential and time of electrophoresis)
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Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes Mutagenesis (IF 2.7) Pub Date : 2023-05-25 Andrea Pirković, Vesna Lazić, Biljana Spremo-Potparević, Lada Živković, Dijana Topalović, Sanja Kuzman, Jelena Antić-Stanković, Dragana Božić, Milica Jovanović-Krivokuća, Jovan M Nedeljković
Dry olive leaf extract (DOLE) and its active component oleuropein (OLE) were applied as reducing and stabilizing agents to prepare colloidal 20-25 nm silver nanoparticles (Ag NPs). The Ag NPs were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The cytotoxic actions of coated Ag NPs, and their inorganic and organic components, were examined
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Evaluation of endpoints for the study and diagnosis of mitochondrial toxicity and disease: A narrative review Mutagenesis (IF 2.7) Pub Date : 2023-05-05 Prashamsa Gharti, Jessica F Fletcher, Katherine E Chapman
Mitochondrial DNA mutation and toxicity have been linked to several inherited and acquired diseases; however, these are challenging to diagnose and characterise due to clinical and genetic heterogeneity. This review investigates current techniques for the analysis of mitochondrial perturbations, and novel, emerging endpoints for routine application within the clinical setting. Particular focus is given
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Reconstructed human intestinal comet assay, a possible alternative in vitro model for genotoxicity assessment Mutagenesis (IF 2.7) Pub Date : 2023-04-28 Christopher Owen Hughes, Hui Kheng Lim, Joseph Choon Wee Tan, David Ian Leavesley, Benjamin Paul Chapman Smith
The aim of the present study was to evaluate the compatibility of reconstructed 3D human small intestinal microtissues to perform the in vitro comet assay. The comet assay is a common follow-up genotoxicity test to confirm or supplement other genotoxicity data. Technically, it can be performed utilising a range of in vitro and in vivo assay systems. Here, we have developed a new reconstructed human
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Announcing the UKEMS Next Generation Sequencing special interest group (NGS SIG). Mutagenesis (IF 2.7) Pub Date : 2023-06-20 Anthony Lynch,Darren Kidd,Anne Ashford
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Measuring blood cell DNA damage using the PIG-A mutation and CBMN assay in pancreatic cancer patients: a pilot study Mutagenesis (IF 2.7) Pub Date : 2023-04-03 Lucy Nichols, Rachel Lawrence, Hasan Haboubi, Bilal Al-Sarireh, Shareen Doak, Gareth Jenkins
Pancreatic cancer still has one of the worst prognoses of all solid malignancies, despite developments in cancer knowledge and care. Research into pancreatic cancer has not fully translated into clinical improvements and as a result, fewer than 1% of patients survive 10-years post-diagnosis. This bleak outlook for patients could be improved by earlier diagnosis. The human erythrocyte phosphatidylinositol
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Effects of folate on telomere length and chromosome stability of human fibroblasts and melanoma cells in vitro: A comparison of folic acid and 5-methyltetrahydrofolate Mutagenesis (IF 2.7) Pub Date : 2023-03-18 Han Wang, Juan Ni, Xihan Guo, Jinglun Xue, Xu Wang
Telomere length (TL), which is maintained by hTERT (component of telomerase) and/or TRF1/TRF2 (core components of shelterin) via different mechanisms, is essential for chromosomal stability and cell survival. Folates comprise a group of essential B9 vitamin that involve in DNA synthesis and methylation. This study aimed to evaluate the effects of folic acid (FA) and 5-methyltetrahydrofolate (5-MeTHF)
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Investigation Of Genotoxic Effect Of Octyl Gallate Used As An Antioxidant Food Additive In In Vitro Test Systems Mutagenesis (IF 2.7) Pub Date : 2023-03-08 Ece Avuloglu Yilmaz, Deniz Yuzbasioglu, Fatma Unal
Several antioxidant food additives are added to oils, soups, sauces, chewing gum, potato chips etc. One of them is octyl gallate. The purpose of this study was to evaluate the potential genotoxicity of octyl gallate in human lymphocytes, using in vitro chromosomal abnormalities (CA), sister chromatid exchange (SCE), cytokinesis block micronucleus cytome (CBMN-Cyt), micronucleus-FISH (MN-FISH), and
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Functional analysis of polymorphism haplotypes of MGMT in residents of high background radiation area. Mutagenesis (IF 2.7) Pub Date : 2023-05-12 Linqian Zhou,Sufen Zhang,WeiXu Huang,Lingyu Zhang,Yashi Cai,Weiyi Ke,Lina Cai,Jianming Zou,Huifeng Chen
To investigate the distribution of polymorphisms and their frequent haplotypes in the regulatory region of MGMT in residents of high background radiation area (HBRA) and their impacts on transcriptional activity, we collected DNA samples from 83 healthy Chinese residents in HBRA and searched for genetic polymorphisms in the regulatory region of MGMT. Haplotypes were characterized by Haploview analysis
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Dry tobacco leaves: an in vivo and in silico approach to the consequences of occupational exposure. Mutagenesis (IF 2.7) Pub Date : 2023-05-12 Daiana Dalberto,Ana L H Garcia,Melissa R De Souza,Juliana Picinini,Solange Soares,Guilherme M S De Souza,Paola Chytry,Johnny F Dias,Mirian Salvador,Fernanda R Da Silva,Juliana Da Silva
Exposure of tobacco workers handling dried tobacco leaves has been linked to an increased risk of toxicity and respiratory illness due to the presence of nicotine and other chemicals. This study aimed to evaluate the DNA damage caused by the exposure of tobacco growers during the dry leaf classification process and the relation to cellular mechanisms. A total of 86 individuals participated in the study
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Genome instability in peripheral blood lymphocytes of patients with heart failure and reduced ejection fraction Mutagenesis (IF 2.7) Pub Date : 2023-02-04 Jovana Tubić Vukajlović, Ivan Simić, Zorica Smiljanić, Darko Grujičić, Olivera Milošević-Djordjević
Heart failure (HF) is a complex clinical condition characterized by functional and structural defects of the myocardium, but genetic and environmental factors are considered to play an important role in the development of the disease. In the present study, we investigated the genome instability (DNA and chromosomal damage) in patients with heart failure with reduced ejection fraction (HFrEF) ≤40% and
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EEMGS New Investigators: rising stars in environmental mutagenesis. Mutagenesis (IF 2.7) Pub Date : 2023-02-03 Ezgi Eyluel Bankoglu,Fiona Chapman,Marko Gerić
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Impact of experimental design factors on the potency of genotoxicants in in vitro tests. Mutagenesis (IF 2.7) Pub Date : 2022-12-08 Julie Sanders,Anouck Thienpont,Roel Anthonissen,Tamara Vanhaecke,Birgit Mertens
Previous studies have shown that differences in experimental design factors may alter the potency of genotoxic compounds in in vitro genotoxicity tests. Most of these studies used traditional statistical methods based on the lowest observed genotoxic effect levels, whereas more appropriate methods, such as the benchmark dose (BMD) approach, are now available to compare genotoxic potencies under different
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Ex vivo explant model of adenoma and colorectal cancer to explore mechanisms of action and patient response to cancer prevention therapies. Mutagenesis (IF 2.7) Pub Date : 2022-12-08 Sam Khan,Gareth J Miles,Constantinos Demetriou,Zahirah Sidat,Nalini Foreman,Kevin West,Ankur Karmokar,Lynne Howells,Catrin Pritchard,Anne L Thomas,Karen Brown
Colorectal cancer (CRC) is the second leading cause of cancer death in the UK. Novel therapeutic prevention strategies to inhibit the development and progression of CRC would be invaluable. Potential contenders include low toxicity agents such as dietary-derived agents or repurposed drugs. However, in vitro and in vivo models used in drug development often do not take into account the heterogeneity
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In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach-the Joint Danube Survey 4 Case Study. Mutagenesis (IF 2.7) Pub Date : 2023-02-03 Jovana Jovanović Marić,Stoimir Kolarević,Jelena Đorđević,Karolina Sunjog,Ivan Nikolić,Ana Marić,Marija Ilić,Predrag Simonović,Nikiforos Alygizakis,Kelsey Ng,Peter Oswald,Jaroslav Slobodnik,Bojana Žegura,Branka Vuković-Gačić,Momir Paunović,Margareta Kračun-Kolarević
Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus
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Air pollution in Sarajevo, Bosnia and Herzegovina, assessed by plant comet assay. Mutagenesis (IF 2.7) Pub Date : 2023-02-03 Mujo Hasanovic,Tamara Cetkovic,Bertrand Pourrut,Lejla Caluk Klacar,Maida Hadzic Omanovic,Adaleta Durmic-Pasic,Sanin Haveric,Anja Haveric
Bosnia and Herzegovina (B&H) is among the European countries with the highest rate of air pollution-related death cases and the poorest air quality. The main causes are solid fuel consumption, traffic, and the poorly developed or implemented air pollution reduction policies. In addition, the city of Sarajevo, the capital of B&H, suffers temperature inversion episodes in autumn/winter months, which
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Probiotic bacteria isolated from fermented meat displays high antioxidant and anti-inflammatory potential. Mutagenesis (IF 2.7) Pub Date : 2023-02-03 Deni Kostelac,Marko Gerić,Goran Gajski,Jadranka Frece
One of the ways to impact emerging problems of unhealthy diet such as microbiota dysbiosis, inflammation, and oxidative stress is the application of probiotics and their incorporation into different food matrices. Discovery and selection of appropriate probiotic bacteria is challenging procedure especially for fermented meat products that have also been described as a potential source of resilient
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In vitro genotoxicity assessment of French fries from mass catering companies: a preliminary study Mutagenesis (IF 2.7) Pub Date : 2022-10-15 Julen Sanz-Serrano, Roncesvalles Garayoa, Ana Isabel Vitas, Adela López de Cerain, Amaya Azqueta
It is generally assumed that French fries are likely to have weak in vitro mutagenic activity, but most studies thereof have only assessed gene mutations. In this article, the genotoxicity of 10 extracts of French fries was assessed using the in vitro micronucleus test (following the principles of the OECD 487 guidelines). Each sample was obtained from a different mass catering company in Navarra (Spain)
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Interspecific differences in oxidative DNA damage after hydrogen peroxide exposure of sea urchin coelomocytes. Mutagenesis (IF 2.7) Pub Date : 2023-02-03 Fengjia Liu,Kim S Last,Theodore B Henry,Helena C Reinardy
Interspecific comparison of DNA damage can provide information on the relative vulnerability of marine organisms to toxicants that induce oxidative genotoxicity. Hydrogen peroxide (H2O2) is an oxidative toxicant that causes DNA strand breaks and nucleotide oxidation and is used in multiple industries including Atlantic salmon aquaculture to treat infestations of ectoparasitic sea lice. H2O2 (up to
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A pilot biomonitoring study of air pollution in the urban area of Sarajevo, Bosnia and Herzegovina: genotoxicity assessment in buccal cells. Mutagenesis (IF 2.7) Pub Date : 2023-02-03 Tamara Cetkovic,Anja Haveric,Selma Behmen,Maida Hadzic Omanovic,Lejla Caluk Klacar,Alen Dzaferspahic,Irma Durmisevic,Mahira Mehanovic,Sanin Haveric
Air pollution, recognized as a human carcinogen, is a significant cause of death in industrial and developing countries, and Bosnia and Herzegovina (B&H) is one of the leading countries for air pollution-caused death rate and has the poorest urban air quality in Europe. Despite a population decrease, urban air pollution in B&H has increased due to traffic pollution and still intensive use of solid
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The roles of mutated SPINK1 gene in prostate cancer cells. Mutagenesis (IF 2.7) Pub Date : 2022-12-08 Xiuyi Pan,Junya Tan,Xiaoxue Yin,Qianqi Liu,Linmao Zheng,Zhengzheng Su,Qiao Zhou,Ni Chen
SPINK1-positive prostate cancer (PCa) has been identified as an aggressive PCa subtype. However, there is a lack of definite studies to elucidate the underlying mechanism of the loss of SPINK1 expression in most PCa cells except 22Rv1 cells, which are derived from a human prostatic carcinoma xenograft, CWR22R. The aim of this study was to investigate the mechanisms of SPINK1 protein positive/negative
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Genome-wide analysis of DNaseI hypersensitivity unveils open chromatin associated with histone H3 modifications after areca nut with lime exposure. Mutagenesis (IF 2.7) Pub Date : 2022-10-26 Pooja Swargiary,Nabamita Boruah,Chongtham Sovachandra Singh,Anupam Chatterjee
Research over the years revealed that precocious anaphase, securin overexpression, and genome instability in both target and nontarget cells are significantly associated with the increased risk of areca nut (AN) and lime-induced oral, esophageal, and gastric cancers. Further, hyperphosphorylation of Rb and histone H3 epigenetic modifications both globally and in the promoter region of the securin gene
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Cytoprotective and genoprotective effects of taxifolin against oxidative damage in HTR-8/SVneo human trophoblast cells Mutagenesis (IF 2.7) Pub Date : 2022-09-09 Marija Bruić, Andrea Pirković, Aleksandra Vilotić, Milica Jovanović-Krivokuća, Biljana Spremo-Potparević
An increase of reactive oxygen species in the placenta and oxidative disbalance has been recognized as a significant factor contributing to pregnancy complications. Dietary intake of food rich in antioxidants during pregnancy could exert a protective role in the prevention of adverse outcomes such as preeclampsia, miscarriage, and others. Flavonoid taxifolin has shown numerous health-promoting effects
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Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis) Mutagenesis (IF 2.7) Pub Date : 2022-09-09 Stoimir Kolarević, Margareta Kračun-Kolarević, Jovana Jovanović Marić, Jelena Djordjević, Branka Vuković-Gačić, Danijela Joksimović, Rajko Martinović, Oliver Bajt, Andreja Ramšak
In this study, the possible ‘vector effect’ within the exposure of Mediterranean mussels (Mytilus galloprovincialis) to polystyrene microplastics with adsorbed fluoranthene was investigated by applying the multibiomarker approach. The major focus was placed on genotoxicological endpoints as to our knowledge there are no literature data on the genotoxicity of polystyrene microparticles alone or with
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Transferability and reproducibility of the EpiSkin™ Micronucleus Assay Mutagenesis (IF 2.7) Pub Date : 2022-09-06 Lizao Chen, Fang Huang, CaiChun Kei, Jinsong Zhang, Jing Sang, Ying Yang, Rong Kuang, Xikun Xiong, Qing Li, Yanfeng Liu, Qin Qin, E Zhao, Nathalie Alépée, Gladys Ouedraogo, Nan Li, Zhenzi Cai
A novel in vitro 3D micronucleus assay was developed in China using the EpiSkin™ 3D human skin model. This EpiSkin™ Micronucleus Assay showed good predictivity and reproducibility during internal validation and is expected to contribute to in vitro genotoxicity testing as a follow-up for positive results from 2D micronucleus assay. Having developed the assay in one laboratory, further work focused
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Characterization of benzo[a]pyrene and colchicine based on an in vivo repeat-dosing multi-endpoint genotoxicity quantitative assessment platform Mutagenesis (IF 2.7) Pub Date : 2022-07-23 Zhu Zeng, Jiao Huo, Xuejiao Zhu, Yunjie Liu, Ruirui Li, Yiyi Chen, Lishi Zhang, Jinyao Chen
Two prototypical genotoxicants, benzo[a]pyrene (B[a]P) and colchicine (COL), were selected as model compounds to deduce their quantitative genotoxic dose–response relationship at low doses in a multi-endpoint genotoxicity assessment platform. Male Sprague-Dawley rats were treated with B[a]P (2.5–80 mg/kg bw/day) and COL (0.125–2 mg/kg bw/day) daily for 28 days. The parameters included were as follows:
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Optimizing machine-learning models for mutagenicity prediction through better feature selection. Mutagenesis (IF 2.7) Pub Date : 2022-10-26 Nicolas K Shinada,Naoki Koyama,Megumi Ikemori,Tomoki Nishioka,Seiji Hitaoka,Atsushi Hakura,Shoji Asakura,Yukiko Matsuoka,Sucheendra K Palaniappan
Assessing a compound's mutagenicity using machine learning is an important activity in the drug discovery and development process. Traditional methods of mutagenicity detection, such as Ames test, are expensive and time and labor intensive. In this context, in silico methods that predict a compound mutagenicity with high accuracy are important. Recently, machine-learning (ML) models are increasingly
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DNA damage in peripheral blood lymphocytes of severely ill COVID-19 patients in relation to inflammatory markers and parameters of hemostasis. Mutagenesis (IF 2.7) Pub Date : 2022-10-26 Olgica Mihaljevic,Snezana Zivancevic-Simonovic,Vojislav Cupurdija,Milos Marinkovic,Jovana Tubic Vukajlovic,Aleksandra Markovic,Marijana Stanojevic-Pirkovic,Olivera Milosevic-Djordjevic
Bearing in the mind that a variety of agents can contribute to genome instability, including viral infections, the aim of this study was to analyze DNA damage in hospitalized COVID-19 patients and its relationship with certain laboratory parameters. The potential impact of applied therapy and chest X-rays on DNA damage was also estimated. The study population included 24 severely COVID-19 patients