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The development of sex differences in the nervous system and behavior of flies, worms, and rodents Dev. Biol. (IF 2.895) Pub Date : 2021-01-20 Kristen L. Meeh; Clare T. Rickel; Alexander J. Sansano; Troy R. Shirangi
Understanding how sex differences in innate animal behaviors arise has long fascinated biologists. As a general rule, the potential for sex differences in behavior is built by the developmental actions of sex-specific hormones or regulatory proteins that direct the sexual differentiation of the nervous system. In the last decade, studies in several animal systems have uncovered neural circuit mechanisms
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Nutrient availability contributes to a graded refractory period for regeneration in Xenopus tropicalis Dev. Biol. (IF 2.895) Pub Date : 2021-01-20 Madison C. Williams; Jeet H. Patel; Anneke D. Kakebeen; Andrea E. Wills
Xenopus tadpoles are a unique model for regeneration in that they exhibit two distinct phases of age-specific regenerative competence. In Xenopus laevis, young tadpoles fully regenerate following major injuries such as tail transection, then transiently lose regenerative competence during the “refractory period” from stages 45-47. Regenerative competence is then regained in older tadpoles before being
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Human gastrulation: The embryo and its models Dev. Biol. (IF 2.895) Pub Date : 2021-01-20 Sabitri Ghimire; Veronika Mantziou; Naomi Moris; Alfonso Martinez Arias
Technical and ethical limitations create a challenge to study early human development, especially following the first 3 weeks of development after fertilization, when the fundamental aspects of the body plan are established through the process called gastrulation. As a consequence, our current understanding of human development is mostly based on the anatomical and histological studies on Carnegie
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Coup-TF: A maternal factor essential for differentiation along the embryonic axes in the sea urchin Paracentrotus lividus Dev. Biol. (IF 2.895) Pub Date : 2021-01-20 Ioannis Tsironis; Periklis Paganos; Georgia Gouvi; Panagiotis Tsimpos; Andriana Stamopoulou; Maria Ina Arnone; Constantin N. Flytzanis
Coup-TF, a member of the nuclear receptor super-family, is present in the pool of maternal mRNAs and proteins in the sea urchin egg. The presence of this protein seems to be essential for the execution of the early developmental program, leading to all three embryonic layers. Our results demonstrate that Pl-Coup-TF morphants, i.e. Pl-Coup-TF morpholino knockdown embryos, resemble blastulae that lack
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microRNA-31 regulates skeletogenesis by direct suppression of Eve and Wnt1 Dev. Biol. (IF 2.895) Pub Date : 2021-01-20 Nina Faye Sampilo; Nadezda A. Stepicheva; Jia L. Song
microRNAs (miRNAs) play a critical role in a variety of biological processes, including embryogenesis and the physiological functions of cells. Evolutionarily conserved microRNA-31 (miR-31) has been found to be involved in cancer, bone formation, and lymphatic development. We previously discovered that, in the sea urchin, miR-31 knockdown (KD) embryos have shortened dorsoventral connecting rods, mispatterned
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The FGF-AKT pathway is necessary for cardiomyocyte survival for heart regeneration in zebrafish Dev. Biol. (IF 2.895) Pub Date : 2021-01-11 Naoyuki Tahara; Ryutaro Akiyama; Justin Wang; Hiroko Kawakami; Yasumasa Bessho; Yasuhiko Kawakami
Zebrafish have a remarkable ability to regenerate the myocardium after injury by proliferation of pre-existing cardiomyocytes. Fibroblast growth factor (FGF) signaling is known to play a critical role in zebrafish heart regeneration through promotion of neovascularization of the regenerating myocardium. Here, we define an additional function of FGF signaling in the zebrafish myocardium after injury
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Synthetic by design: Exploiting tissue self-organization to explore early human embryology Dev. Biol. (IF 2.895) Pub Date : 2021-01-19 Rosado-Olivieri; Edwin A. Brivanlou; H. Ali
Recent advances in synthetic human embryology has provided a previously inexistent molecular portrait of human development. Models of synthetic human embryonic tissues capitalize on the self-organizing capabilities of human embryonic stem cells when they are cultured on biomimetic conditions that simulate in vivo human development. In this Review, we discuss these models and how they have shed light
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Anterior lateral plate mesoderm gives rise to multiple tissues and requires tbx5a function in left-right asymmetry, migration dynamics, and cell specification of late-addition cardiac cells. Dev. Biol. (IF 2.895) Pub Date : 2021-01-19 Lindsey M.F. Mao; Erin A.T. Boyle Anderson; Robert K. Ho
In this study, we elucidate a single cell resolution fate map in the zebrafish in a sub-section of the anterior Lateral Plate Mesoderm (aLPM) at 18 hpf. Our results show that this tissue is not organized into segregated regions but gives rise to intermingled pericardial sac, peritoneum, pharyngeal arch and cardiac precursors. We further report upon asymmetrical contributions of lateral aLPM-derived
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CRISPR-Cas9 editing of non-coding genomic loci as a means of controlling gene expression in the sea urchin Dev. Biol. (IF 2.895) Pub Date : 2021-01-19 Alice Pieplow; Meseret Dastaw; Tetsushi Sakuma; Naoaki Sakamoto; Takashi Yamamoto; Mamiko Yajima; Nathalie Oulhen; Gary M. Wessel
We seek to manipulate gene function here through CRISPR-Cas9 editing of cis-regulatory sequences, rather than the more typical mutation of coding regions. This approach would minimize secondary effects of cellular responses to nonsense mediated decay pathways or to mutant protein products by premature stops. This strategy also allows for reducing gene activity in cases where a complete gene knockout
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Multiple roles for Pax2 in the embryonic mouse eye Dev. Biol. (IF 2.895) Pub Date : 2021-01-09 Bernadett Bosze; Julissa Suarez-Navarro; Abdul Soofi; James D. Lauderdale; Gregory R. Dressler; Nadean L. Brown
The vertebrate eye anlage grows out of the brain and folds into bilayered optic cups. The eye is patterned along multiple axes, precisely controlled by genetic programs, to delineate neural retina, pigment epithelium, and optic stalk tissues. Pax genes encode developmental regulators of key morphogenetic events, with Pax2 being essential for interpreting inductive signals, including in the eye. PAX2
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GRDN-1/girdin regulates dendrite morphogenesis and cilium position in two specialized sensory neuron types in C. elegans Dev. Biol. (IF 2.895) Pub Date : 2021-01-16 Inna Nechipurenko; Sofia Lavrentyeva; Piali Sengupta
Primary cilia are located at the dendritic tips of sensory neurons and house the molecular machinery necessary for detection and transduction of sensory stimuli. The mechanisms that coordinate dendrite extension with cilium position during sensory neuron development are not well understood. Here, we show that GRDN-1, the Caenorhabditis elegans ortholog of the highly conserved scaffold and signaling
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Anomalous incisor morphology indicates tissue-specific roles for Tfap2a and Tfap2b in tooth development Dev. Biol. (IF 2.895) Pub Date : 2021-01-15 Emily D. Woodruff; Galaxy C. Gutierrez; Eric Van Otterloo; Trevor Williams; Martin J. Cohn
Mice possess two types of teeth that differ in their cusp patterns; incisors have one cusp and molars have multiple cusps. The patterning of these two types of teeth relies on fine-tuning of the reciprocal molecular signaling between dental epithelial and mesenchymal tissues during embryonic development. The AP-2 transcription factors, particularly Tfap2a and Tfap2b, are essential components of such
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Early patterning of ABCB, ABCC, and ABCG transporters establishes unique territories of small molecule transport in the embryonic mesoderm and endoderm. Dev. Biol. (IF 2.895) Pub Date : 2021-01-15 Catherine S. Schrankel; Amro Hamdoun
Directed intercellular movement of diverse small molecules, including metabolites, signal molecules and xenobiotics, is a key feature of multicellularity. Networks of small molecule transporters (SMTs), including several ATP Binding Cassette (ABC) transporters, are central to this process. While small molecule transporters are well described in differentiated organs, little is known about their patterns
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ETS factors are required but not sufficient for specific patterns of enhancer activity in different endothelial subtypes Dev. Biol. (IF 2.895) Pub Date : 2021-01-14 Alice Neal; Svanhild Nornes; Pakavarin Louphrasitthiphol; Natalia Sacilotto; Mark D. Preston; Lucija Fleisinger; Sophie Payne; Sarah De Val
Correct vascular differentiation requires distinct patterns of gene expression in different subtypes of endothelial cells. Members of the ETS transcription factor family are essential for the transcriptional activation of arterial and angiogenesis-specific gene regulatory elements, leading to the hypothesis that they play lineage-defining roles in arterial and angiogenic differentiation directly downstream
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A globin-family protein, Cytoglobin 1, is involved in the development of neural crest-derived tissues and organs in zebrafish Dev. Biol. (IF 2.895) Pub Date : 2021-01-11 Kazuki Takahashi; Yuki Ito; Mami Yoshimura; Masataka Nikaido; Tatsuya Yuikawa; Akinori Kawamura; Sachiko Tsuda; Daichi Kage; Kyo Yamasu
The zebrafish is an excellent model animal that is amenable to forward genetics approaches. To uncover unknown developmental regulatory mechanisms in vertebrates, we conducted chemical mutagenesis screening and identified a novel mutation, kanazutsi (kzt). This mutation is recessive, and its homozygotes are embryonic lethal. Mutant embryos suffered from a variety of morphological defects, such as head
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YAP1 and its fusion proteins in cancer initiation, progression and therapeutic resistance Dev. Biol. (IF 2.895) Pub Date : 2021-01-08 Frank Szulzewsky; Eric C. Holland; Valeri Vasioukhin
YAP1 is a transcriptional co-activator whose activity is controlled by the Hippo signaling pathway. In addition to important functions in normal tissue homeostasis and regeneration, YAP1 has also prominent functions in cancer initiation, aggressiveness, metastasis, and therapy resistance. In this review we are discussing the molecular functions of YAP1 and its roles in cancer, with a focus on the different
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Sox10-cre BAC transgenes reveal temporal restriction of mesenchymal cranial neural crest and identify glandular Sox10 expression Dev. Biol. (IF 2.895) Pub Date : 2020-12-13 Karen K. Deal; Jennifer C. Rosebrock; Angela M. Eeds; Jean-Marc L. DeKeyser; Melissa A. Musser; Sara J. Ireland; Aaron A. May-Zhang; Dennis P. Buehler; E. Michelle Southard-Smith
Diversity of neural crest derivatives has been studied with a variety of approaches during embryonic development. In mammals Cre-LoxP lineage tracing is a robust means to fate map neural crest relying on cre driven from regulatory elements of early neural crest genes. Sox10 is an essential transcription factor for normal neural crest development. A variety of efforts have been made to label neural
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Modeling mammalian trunk development in a dish Dev. Biol. (IF 2.895) Pub Date : 2020-12-29 Jesse V. Veenvliet; Bernhard G. Herrmann
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Is zebrafish heart regeneration “complete”? Lineage-restricted cardiomyocytes proliferate to pre-injury numbers but some fail to differentiate in fibrotic hearts Dev. Biol. (IF 2.895) Pub Date : 2020-12-10 Alberto Bertozzi; Chi-Chung Wu; Phong D. Nguyen; Mohankrishna Dalvoy Vasudevarao; Medhanie A. Mulaw; Charlotte D. Koopman; Teun P. de Boer; Jeroen Bakkers; Gilbert Weidinger
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Physiological electric fields induce directional migration of mammalian cranial neural crest cells Dev. Biol. (IF 2.895) Pub Date : 2020-12-24 Abijeet Singh Mehta; Pin Ha; Kan Zhu; ShiYu Li; Kang Ting; Chia Soo; Xinli Zhang; Min Zhao
During neurulation, cranial neural crest cells (CNCCs) migrate long distances from the neural tube to their terminal site of differentiation. The pathway traveled by the CNCCs defines the blueprint for craniofacial construction, abnormalities of which contribute to three-quarters of human birth defects. Biophysical cues like naturally occurring electric fields (EFs) have been proposed to be one of
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Interdependent regulation of stereotyped and stochastic photoreceptor fates in the fly eye Dev. Biol. (IF 2.895) Pub Date : 2020-12-14 Adam C. Miller; Elizabeth A. Urban; Eric L. Lyons; Tory G. Herman; Robert J. Johnston
Diversification of neuronal subtypes often requires stochastic gene regulatory mechanisms. How stochastically expressed transcription factors interact with other regulators in gene networks to specify cell fates is poorly understood. The random mosaic of color-detecting R7 photoreceptor subtypes in Drosophila is controlled by the stochastic on/off expression of the transcription factor Spineless (Ss)
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Taste buds are not derived from neural crest in mouse, chicken, and zebrafish Dev. Biol. (IF 2.895) Pub Date : 2020-12-14 Wenxin Yu; Zhonghou Wang; Brett Marshall; Yuta Yoshida; Renita Patel; Xiaogang Cui; Rebecca Ball; Linlin Yin; Fuminori Kawabata; Shoji Tabata; Wenbiao Chen; Robert N. Kelsh; James D. Lauderdale; Hong-Xiang Liu
Our lineage tracing studies using multiple Cre mouse lines showed a concurrent labeling of abundant taste bud cells and the underlying connective tissue with a neural crest (NC) origin, warranting a further examination on the issue of whether there is an NC derivation of taste bud cells. In this study, we mapped NC cell lineages in three different models, Sox10-iCreERT2/tdT mouse, GFP+ neural fold
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Reassembling gastrulation Dev. Biol. (IF 2.895) Pub Date : 2020-12-19 Alexandra Schauer; Carl-Philipp Heisenberg
During development, a single cell is transformed into a highly complex organism through progressive cell division, specification and rearrangement. An important prerequisite for the emergence of patterns within the developing organism is to establish asymmetries at various scales, ranging from individual cells to the entire embryo, eventually giving rise to the different body structures. This becomes
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Multi-tissue patterning drives anterior morphogenesis of the C. elegans embryo Dev. Biol. (IF 2.895) Pub Date : 2020-12-10 Stéphanie Grimbert; Karina Mastronardi; Victoria Richard; Ryan Christensen; Christopher Law; Khashayar Zardoui; David Fay; Alisa Piekny
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Kcnb1 plays a role in development of the inner ear Dev. Biol. (IF 2.895) Pub Date : 2020-12-11 Justyna Jedrychowska; Eugene V. Gasanov; Vladimir Korzh
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The dynamics of morphogenesis in stem cell-based embryology: Novel insights for symmetry breaking Dev. Biol. (IF 2.895) Pub Date : 2020-12-15 Berna Sozen; Jake Cornwall-Scoones; Magdalena Zernicka-Goetz
Breaking embryonic symmetry is an essential prerequisite to shape the initially symmetric embryo into a highly organized body plan that serves as the blueprint of the adult organism. This critical process is driven by morphogen signaling gradients that instruct anteroposterior axis specification. Despite its fundamental importance, what triggers symmetry breaking and how the signaling gradients are
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Modeling human embryo development with embryonic and extra-embryonic stem cells Dev. Biol. (IF 2.895) Pub Date : 2020-12-14 Bailey A.T. Weatherbee; Tongtong Cui; Magdalena Zernicka-Goetz
Early human post-implantation development involves extensive growth combined with a series of complex morphogenetic events. The lack of precise spatial and temporal control over these processes leads to pregnancy loss. Given the ethical and technical limitations in studying the natural human embryo, alternative approaches are needed to investigate mechanisms underlying this critical stage of human
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Returning to kidney development to deliver synthetic kidneys Dev. Biol. (IF 2.895) Pub Date : 2020-12-14 Melissa H. Little
There is no doubt that the development of transplantable synthetic kidneys could improve the outcome for the many millions of people worldwide suffering from chronic kidney disease. Substantial progress has been made in the last 6 years in the generation of kidney tissue from stem cells. However, the limited scale, incomplete cellular complexity and functional immaturity of such structures suggests
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Stressful development: integrating endoderm development, stress, and longevity Dev. Biol. (IF 2.895) Pub Date : 2020-12-08 Chee Kiang Ewe; Geneva Alok; Joel H. Rothman
In addition to performing digestion and nutrient absorption, the intestine serves as one of the first barriers to the external environment, crucial for protecting the host from environmental toxins, pathogenic invaders, and other stress inducers. The gene regulatory network (GRN) governing embryonic development of the endoderm and subsequent differentiation and maintenance of the intestine has been
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Postembryonic screen for mutations affecting spine development in zebrafish Dev. Biol. (IF 2.895) Pub Date : 2020-12-05 Ryan S. Gray; Roberto Gonzalez; Sarah D. Ackerman; Ryoko Minowa; Johanna F. Griest; Melisa N. Bayrak; Benjamin Troutwine; Stephen Canter; Kelly R. Monk; Diane S. Sepich; Lilianna Solnica-Krezel
The spine gives structural support for the adult body, protects the spinal cord, and provides muscle attachment for moving through the environment. The development and maturation of the spine and its physiology involve the integration of multiple musculoskeletal tissues including bone, cartilage, and fibrocartilaginous joints, as well as innervation and control by the nervous system. One of the most
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Midline morphogenesis of zebrafish foregut endoderm is dependent on Hoxb5b Dev. Biol. (IF 2.895) Pub Date : 2020-12-05 Gökhan Dalgin; Victoria E. Prince
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A role for Cep70 in centriole amplification in multiciliated cells Dev. Biol. (IF 2.895) Pub Date : 2020-12-04 Sun K. Kim; Eva Brotslaw; Virginie Thome; Jen Mitchell; Rosa Ventrella; Caitlin Collins; Brian Mitchell
Centriole amplification in multiciliated cells occurs in a pseudo-cell cycle regulated process that typically utilizes a poorly characterized molecularly dense structure called the deuterosome. We identified the centrosomal protein Cep70 as a novel deuterosome-associated protein that forms a complex with other deuterosome proteins, CCDC78 and Deup1. Cep70 dynamically associates with deuterosomes during
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Dysregulated BMP signaling through ACVR1 impairs digit joint development in fibrodysplasia ossificans progressiva (FOP) Dev. Biol. (IF 2.895) Pub Date : 2020-11-17 O. Will Towler; Sun H. Peck; Frederick S. Kaplan; Eileen M. Shore
The development of joints in the mammalian skeleton depends on the precise regulation of multiple interacting signaling pathways including the bone morphogenetic protein (BMP) pathway, a key regulator of joint development, digit patterning, skeletal growth, and chondrogenesis. Mutations in the BMP receptor ACVR1 cause the rare genetic disease fibrodysplasia ossificans progressiva (FOP) in which extensive
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Loss of Smad4 in the scleraxis cell lineage results in postnatal joint contracture Dev. Biol. (IF 2.895) Pub Date : 2020-11-25 Saundra Y. Schlesinger; Seongkyung Seo; Brian A. Pryce; Sara F. Tufa; Douglas R. Keene; Alice H. Huang; Ronen Schweitzer
Growth of the musculoskeletal system requires precise coordination between bone, muscle, and tendon during development. Insufficient elongation of the muscle-tendon unit relative to bone growth results in joint contracture, a condition characterized by reduction or complete loss of joint range of motion. Here we establish a novel murine model of joint contracture by targeting Smad4 for deletion in
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Mutations in Drosophila crinkled/Myosin VIIA disrupt denticle morphogenesis Dev. Biol. (IF 2.895) Pub Date : 2020-11-25 Jennifer L. Sallee; Janice M. Crawford; Vinay Singh; Daniel P. Kiehart
Actin filament crosslinking, bundling and molecular motor proteins are necessary for the assembly of epithelial projections such as microvilli, stereocilia, hairs, and bristles. Mutations in such proteins cause defects in the shape, structure, and function of these actin - based protrusions. One protein necessary for stereocilia formation, Myosin VIIA, is an actin - based motor protein conserved throughout
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Temperature-Induced uncoupling of cell cycle regulators Dev. Biol. (IF 2.895) Pub Date : 2020-12-03 Hanieh Falahati; Woonyung Hur; Stefano Di Talia; Eric Wieschaus
The early stages of development involve complex sequences of morphological changes that are both reproducible from embryo to embryo and often robust to environmental variability. To investigate the relationship between reproducibility and robustness we examined cell cycle progression in early Drosophila embryos at different temperatures. Our experiments show that while the subdivision of cell cycle
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Mef2c factors are required for early but not late addition of cardiomyocytes to the ventricle Dev. Biol. (IF 2.895) Pub Date : 2020-11-25 Duvaraka Kula-Alwar; Michael S. Marber; Simon M. Hughes; Yaniv Hinits
During heart formation, the heart grows and undergoes dramatic morphogenesis to achieve efficient embryonic function. Both in fish and amniotes, much of the growth occurring after initial heart tube formation arises from second heart field (SHF)-derived progenitor cell addition to the arterial pole, allowing chamber formation. In zebrafish, this process has been extensively studied during embryonic
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BMP signalling is required for extra-embryonic ectoderm development during pre-to-post-implantation transition of the mouse embryo Dev. Biol. (IF 2.895) Pub Date : 2020-11-17 Berna Sozen; Necdet Demir; Magdalena Zernicka-Goetz
At implantation, the mouse embryo undergoes a critical transformation which requires the precise spatiotemporal control of signalling pathways necessary for morphogenesis and developmental progression. The role played by such signalling pathways during this transition are largely unexplored, due to the inaccessibility of the embryo during the implantation when it becomes engulfed by uterine tissues
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Mutations in non-muscle myosin 2A disrupt the actomyosin cytoskeleton in Sertoli cells and cause male infertility Dev. Biol. (IF 2.895) Pub Date : 2020-11-12 Derek C. Sung; Mohsin Ahmad; Connie B. Lerma Cervantes; Yingfan Zhang; Robert S. Adelstein; Xuefei Ma
Mutations in non-muscle myosin 2A (NM2A) encompass a wide spectrum of anomalies collectively known as MYH9-Related Disease (MYH9-RD) in humans that can include macrothrombocytopenia, glomerulosclerosis, deafness, and cataracts. We previously created mouse models of the three mutations most frequently found in humans: R702C, D1424N, and E1841K. While homozygous R702C and D1424N mutations are embryonic
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Molecular detection of maturation stages in the developing kidney Dev. Biol. (IF 2.895) Pub Date : 2020-11-14 Hidekazu Naganuma; Koichiro Miike; Tomoko Ohmori; Shunsuke Tanigawa; Takumi Ichikawa; Mariko Yamane; Masatoshi Eto; Hitoshi Niwa; Akio Kobayashi; Ryuichi Nishinakamura
Recent advances in stem cell biology have enabled the generation of kidney organoids in vitro, and further maturation of these organoids is observed after experimental transplantation. However, the current organoids remain immature and their precise maturation stages are difficult to determine because of limited information on developmental stage-dependent gene expressions in the kidney in vivo. To
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Trim-Away mediated knock down uncovers a new function for Lbh during gastrulation of Xenopus laevis. Dev. Biol. (IF 2.895) Pub Date : 2020-11-04 Emma Weir; Gretchen McLinden; Dominique Alfandari; Hélène Cousin
We previously identified the protein Lbh as necessary for cranial neural crest (CNC) cell migration in Xenopus through the use of morpholinos. However, Lbh is a maternally deposited protein and morpholinos achieve knockdowns through prevention of translation. In order to investigate the role of Lbh in earlier embryonic events, we employed the new technique “Trim-Away” to degrade this maternally deposited
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Non-coding RNAs repressive role in post-transcriptional processing of RUNX2 during the acquisition of the osteogenic phenotype of periodontal ligament mesenchymal stem cells Dev. Biol. (IF 2.895) Pub Date : 2020-11-02 Rahyza I.F. Assis; Geórgia da S. Feltran; Maria Eduarda Salomão Silva; Iasmin Caroline do Rosário Palma; Emanuel Silva Rovai; Taís Browne de Miranda; Marcel Rodrigues Ferreira; Willian F. Zambuzzi; Alexander Birbrair; Denise C. Andia; Rodrigo A. da Silva
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Zebrafish Cdx1b modulates epithalamic asymmetry by regulating ndr2 and lft1 expression Dev. Biol. (IF 2.895) Pub Date : 2020-11-13 Chun-Shiu Wu; Yu-Fen Lu; Yu-Hsiu Liu; Chang-Jen Huang; Sheng-Ping L. Hwang
Nodal signaling is essential for mesoderm and endoderm formation, as well as neural plate induction and establishment of left-right asymmetry. However, the mechanisms controlling expression of Nodal pathway genes in these contexts are not fully known. Previously, we showed that Cdx1b induces expression of downstream Nodal signaling factors during early endoderm formation. In this study, we show that
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The type II integral ER membrane protein VAP-B homolog in C. elegans is cleaved to release the N-terminal MSP domain to signal non-cell-autonomously Dev. Biol. (IF 2.895) Pub Date : 2020-11-05 Hala Zein-Sabatto; Tim Cole; Hieu D. Hoang; Ekta Tiwary; Chenbei Chang; Michael A. Miller
VAMP/synaptobrevin-associated protein B (VAP-B) is a type II ER membrane protein, but its N-terminal MSP domain (MSPd) can be cleaved and secreted. Mutations preventing the cleavage and secretion of MSPd have been implicated in cases of human neurodegenerative diseases. The site of VAP cleavage and the tissues capable in releasing the processed MSPd are not understood. In this study, we analyze the
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The Caudal ParaHox gene is required for hindgut development in the mollusc Tritia (a.k.a. Ilyanassa) Dev. Biol. (IF 2.895) Pub Date : 2020-10-24 Adam B. Johnson; J. David Lambert
Caudal homeobox genes are found across animals, typically linked to two other homeobox genes in what has been called the ParaHox cluster. These genes have been proposed to pattern the anterior-posterior axis of the endoderm ancestrally, but the expression of Caudal in extant groups is varied and often occurs in other germ layers. Here we examine the role of Caudal in the embryo of the mollusc Tritia
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Rethinking embryology in vitro: A synergy between engineering, data science and theory Dev. Biol. (IF 2.895) Pub Date : 2020-11-02 Nicola Gritti; David Oriola; Vikas Trivedi
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Syd/JIP3 controls tissue size by regulating Diap1 protein turnover downstream of Yorkie/YAP Dev. Biol. (IF 2.895) Pub Date : 2020-10-03 Vakil Ahmad; Gangadhar P. Vadla; Chiswili Yves Chabu
How organisms control organ size is not fully understood. We found that Syd/JIP3 is required for proper wing size in Drosophila. JIP3 mutations are associated with organ size defects in mammals. The underlying mechanisms are not well understood. We discovered that Syd/JIP3 inhibition results in a downregulation of the inhibitor of apoptosis protein 1 (Diap1) in the Drosophila wing. Correspondingly
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Tie1 regulates zebrafish cardiac morphogenesis through Tolloid-like 1 expression Dev. Biol. (IF 2.895) Pub Date : 2020-09-21 Claudia Carlantoni; Srinivas Allanki; Zacharias Kontarakis; Andrea Rossi; Janett Piesker; Stefan Günther; Didier Y.R. Stainier
Tie1 is a receptor tyrosine kinase expressed in endothelial cells, where it modulates Angiopoietin/Tie2 signaling. Previous studies have shown that mouse Tie1 mutants exhibit severe cardiovascular defects; however, much remains to be learned about the role of Tie1, especially during cardiac development. To further understand Tie1 function, we generated a zebrafish tie1 mutant line. Homozygous mutant
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Fine-tuning of the PAX-SIX-EYA-DACH network by multiple microRNAs controls embryo myogenesis Dev. Biol. (IF 2.895) Pub Date : 2020-10-17 Camille Viaut; Shannon Weldon; Andrea Münsterberg
MicroRNAs (miRNAs), short non-coding RNAs, which act post-transcriptionally to regulate gene expression, are of widespread significance during development and disease, including muscle disease. Advances in sequencing technology and bioinformatics led to the identification of a large number of miRNAs in vertebrates and other species, however, for many of these miRNAs specific roles have not yet been
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WNT5A-Ca2+-CaN-NFAT signalling plays a permissive role during cartilage differentiation in embryonic chick digit development Dev. Biol. (IF 2.895) Pub Date : 2020-10-12 Alejandro Farrera-Hernández; Jessica Cristina Marín-Llera; Jesús Chimal-Monroy
During digit development, the correct balance of chondrogenic signals ensures the recruitment of undifferentiated cells into the cartilage lineage or the maintenance of cells at the undifferentiated stage. WNT/β catenin maintains the pool of progenitor cells, whereas TGFβ signalling promotes cartilage differentiation by inducing Sox9 expression. Moreover, WNT5A promotes the degradation of β catenin
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Nucleoporin NUP205 plays a critical role in cilia and congenital disease Dev. Biol. (IF 2.895) Pub Date : 2020-10-13 Jonathan Marquez; Dipankan Bhattacharya; C. Patrick Lusk; Mustafa K. Khokha
Ciliopathies affect a variety of tissues during development including the heart, kidneys, respiratory tract, and retina. Though an increasing number of monogenic causes of ciliopathies have been described, many remain unexplained. Recently, recessive variants in NUP93 and NUP205 encoding two proteins of the inner ring of the nuclear pore complex were implicated as causes of steroid resistant nephrotic
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The Neurosphere Simulator: An educational online tool for modeling neural stem cell behavior and tissue growth Dev. Biol. (IF 2.895) Pub Date : 2020-09-28 Günther K.H. Zupanc; Dávid Lehotzky; Isabel P. Tripp
Until very recently, distance education, including digital science labs, served a rather small portion of postsecondary students in the United States and many other countries. This situation has, however, dramatically changed in 2020 in the wake of the COVID-19 pandemic, which forced colleges to rapidly transit from face-to-face instructions to online classes. Here, we report the development of an
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A serpin is required for ectomesoderm, a hallmark of spiralian development Dev. Biol. (IF 2.895) Pub Date : 2020-10-24 Longjun Wu; J. David Lambert
Among animals, diploblasts contain two germ layers, endoderm and ectoderm, while triploblasts have a distinct third germ layer called the mesoderm. Spiralians are a group of triploblast animals that have highly conserved development: they share the distinctive spiralian cleavage pattern as well as a unique source of mesoderm, the ectomesoderm. This population of mesoderm is distinct from endomesoderm
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A transient decrease in mitochondrial activity contributes to establish the ganglion cell fate in retina adapted for high acuity vision Dev. Biol. (IF 2.895) Pub Date : 2020-10-23 Laurent Brodier; Tania Rodrigues; Lidia Matter-Sadzinski; Jean-Marc Matter
Although the plan of the retina is well conserved in vertebrates, there are considerable variations in cell type diversity and number, as well as in the organization and properties of the tissue. The high ratios of retinal ganglion cells (RGCs) to cones in primate fovea and bird retinas favor neural circuits essential for high visual acuity and color vision. The role that cell metabolism could play
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The apical cell – An enigmatic somatic cell in leech ovaries – Structure and putative functions Dev. Biol. (IF 2.895) Pub Date : 2020-10-23 Szymon Gorgoń; Piotr Świątek
Although somatic cells play an integral role in animal gametogenesis, their organization and function are usually poorly characterized, especially in non-model systems. One such example is a peculiar cell found in leech ovaries – the apical cell (AC). A single AC can be found at the apical tip of each ovary cord, the functional unit of leech ovaries, where it is surrounded by other somatic and germline
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There and back again: The mechanisms of differentiation and transdifferentiation in Drosophila blood cells Dev. Biol. (IF 2.895) Pub Date : 2020-10-23 Gábor Csordás; Erika Gábor; Viktor Honti
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In silico analysis of inner ear development using public whole embryonic body single-cell RNA-sequencing data Dev. Biol. (IF 2.895) Pub Date : 2020-10-22 Ryosuke Yamamoto; Hiroe Ohnishi; Koichi Omori; Norio Yamamoto
The inner ear comprises four epithelial domains: the cochlea, vestibule, semicircular canals, and endolymphatic duct/sac. These structures are segregated at embryonic day 13.5 (E13.5). However, these four anatomical structures remain undefined at E10.5. Here, we aimed to identify lineage-specific genes in the early developing inner ear using published data obtained from single-cell RNA-sequencing (scRNA-seq)
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Cloning and characterisation of NMDA receptors in the Pacific oyster, Crassostrea gigas (Thunberg, 1793) in relation to metamorphosis and catecholamine synthesis Dev. Biol. (IF 2.895) Pub Date : 2020-10-22 Susanne Vogeler; Stefano Carboni; Xiaoxu Li; Jacqueline H. Ireland; Penny Miller-Ezzy; Alyssa Joyce
Bivalve metamorphosis is a developmental transition from a free-living larva to a benthic juvenile (spat), regulated by a complex interaction of neurotransmitters and neurohormones such as L-DOPA and epinephrine (catecholamine). We recently suggested an N-Methyl-D-aspartate (NMDA) receptor pathway as an additional and previously unknown regulator of bivalve metamorphosis. To explore this theory further
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Lamellipodia-like protrusions and focal adhesions contribute to collective cell migration in zebrafish Dev. Biol. (IF 2.895) Pub Date : 2020-10-21 Hannah M. Olson; Alex V. Nechiporuk
Collective cell migration is a process where cohorts of cells exhibit coordinated migratory behavior. During individual and collective cellular migration, cells must extend protrusions to interact with the extracellular environment, sense chemotactic cues, and act as points of attachment. The mechanisms and regulators of protrusive behavior have been widely studied in individually migrating cells;
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The developmental biology of kinesins. Dev. Biol. (IF 2.895) Pub Date : 2020-09-19 Mia J Konjikusic,Ryan S Gray,John B Wallingford
Kinesins are microtubule-based motor proteins that are well known for their key roles in cell biological processes ranging from cell division, to intracellular transport of mRNAs, proteins, vesicles, and organelles, and microtubule disassembly. Interestingly, many of the ~45 distinct kinesin genes in vertebrate genomes have also been associated with specific phenotypes in embryonic development. In
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