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Expression analysis of chick Frizzled receptors during spinal cord development Gene Expr. Patterns (IF 0.897) Pub Date : 2021-01-16 Abdulmajeed Fahad Alrefaei; Andrea E. Münsterberg; Grant N. Wheeler
Frizzleds (Fzds) are transmembrane receptors that can transduce signals dependent upon binding of Wnts, a large family of secreted glycoproteins homologous to the Drosophila wingless gene. FZDs are critical for a wide variety of normal and pathological developmental processes. In the nervous system, Wnts and Frizzleds play an important role in anterior-posterior patterning, cell fate decisions, proliferation
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Full Title: miR-302 family, miR-145 and miR-296 temporal expression profile along the cell cycle of human pluripotent stem cells Gene Expr. Patterns (IF 0.897) Pub Date : 2021-01-24 María Soledad Rodríguez-Varela; Sofía Mucci; Guillermo Agustín Videla-Richardson; Luciana Isaja; Gustavo Emilio Sevlever; María Elida Scassa; Leonardo Romorini
Human pluripotent stem cells (hPSCs), like embryonic (hESCs) and induced pluripotent stem cells (hiPSCs), exhibit an unusual cell cycle structure characterized by a short G1 phase and cells being most of time in S phase. hPSCs are receptive to differentiation cues during their transition through G1 phase when lineage determination is decided. Although several MicroRNAs (miRNAs) have been shown to target
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Prediction of Future Gene Expression Profile By Analyzing Its Past Variation Pattern Gene Expr. Patterns (IF 0.897) Pub Date : 2021-01-12 Parinaz Eskandarian; Jamshid Bagherzadeh; Habibollah Pirnejad; Zahra Niazkhani
A number of initial Hematopoietic Stem Cells (HSC) are considered in a container that are able to divide into HSCs or differentiate into various types of descendant cells. In this paper, a method is designed to predict an approximate gene expression profile (GEP) for future descendant cells resulted from HSC division/differentiation. First, the GEP prediction problem is modeled into a multivariate
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phoenixin(smim20), a gene coding for a novel reproductive ligand, is expressed in the brain of adult zebrafish Gene Expr. Patterns (IF 0.897) Pub Date : 2020-12-29 R. Ceriani; C. Calfún; K.E. Whitlock
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Dynamic changes in endoglin expression in the developing mouse heart Gene Expr. Patterns (IF 0.897) Pub Date : 2020-12-26 E. Singh; H.M. Phillips; H.M. Arthur
Endoglin (ENG) is essential for cardiovascular development and is expressed in the heart from its earliest developmental stages. ENG expression has been reported in the cardiac crescent, endocardium, valve mesenchyme and coronary vascular endothelial cells. However, its expression in these cell types is non-uniform and the dynamic changes in ENG expression during heart development have not been systematically
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Cloning, expression and functional analysis of the desert hedgehog (dhh) gene in Chinese tongue sole (Cynoglossus semilaevis) Gene Expr. Patterns (IF 0.897) Pub Date : 2020-12-28 Qian Qi; Zhongdian Dong; Ning Zhang; Liang Wang; Changwei Shao; Wenteng Xu
Desert hedgehog (dhh) is a gene that is crucial for spermatogenesis and Leydig cell differentiation, but little is known regarding its influence on gonadal differentiation and development in fish. To understand its function, we cloned and characterized the dhh gene from Cynoglossus semilaevis (csdhh). The full length csdhh cDNA was 2473 bp, including a 1386 bp open reading frame (ORF), a 475 bp 5′-UTR
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Comparative sequence analysis and functional validation identified a retina-specific enhancer around zic5 and zic2a Gene Expr. Patterns (IF 0.897) Pub Date : 2020-12-28 Qi Zhang; Tingting Li; Yinglan Zhang; Jia Lin; Xudong Chen; Yiting Gui; Qiang Li
The spatiotemporal development of vertebrate retina is regulated by a variety of genes. The zinc finger transcription factors zic5 and zic2a are located close to each other in the chromosome. They have similar expression patterns, and both play important roles in the development of the retina and nervous system. Here, we used ECR browser and gfp fluorescence report experiment to identify a 290bp enhancer
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Cloning and expression of Hoxc6 gene from Pampus argenteus and its relationship with pelvic fin absence Gene Expr. Patterns (IF 0.897) Pub Date : 2020-12-09 Lingzhu Hu; Shun Zhang; Yu Zhou; Kai Liao; Shanliang Xu; Danli Wang
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De Novo Assembly and Transcriptome Analysis of the Momordica charantia Seedlings Responding to Methyl Jasmonate Using 454 Pyrosequencing Gene Expr. Patterns (IF 0.897) Pub Date : 2020-11-28 Shanyong Yi; Xiangwen Song; Wangyang Yu; Rongfei Zhang; Wei Wang; Yucheng Zhao; Bangxing Han; Yanan Gai
Momordica charantia, a medicinal and edible species of the Cucurbitaceae family, has been widely used as a vegetable around the world. Hundreds of pharmacological compounds isolated from the M. charantia have been reported. However, the mechanism of action of the secondary metabolites has not been fully elucidated. In this study, 118,590 unigenes were gained by de novo assembly based on the raw data
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Dact-4 is a Xenopus laevis Spemann organizer gene related to the Dapper/Frodo antagonist of β-catenin family of proteins Gene Expr. Patterns (IF 0.897) Pub Date : 2020-11-10 Gabriele Colozza; Edward M. De Robertis
Dact/Dapper/Frodo members belong to an evolutionarily conserved family of Dishevelled-binding proteins present in mammals, birds, amphibians and fishes that are involved in the regulation of Wnt and TGF-β signaling. In addition to the three established genes (Dact1-3) that compose the Dact family, a fourth paralogue group of related proteins has been recently identified and named Dact-4. Interestingly
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Differential expression pattern of two Brachyury genes in amphioxus embryos Gene Expr. Patterns (IF 0.897) Pub Date : 2020-10-25 Liang Yuan; Yiquan Wang; Guang Li
Cephalochordate amphioxus contain two Brachyury genes (AmphiBra1 and AmphiBra2). Using probes from the highly conserved coding regions, a summation of their expression profiles in amphioxus embryos have been investigated by several previous studies. However, their respective expression patterns have not been determined up to date. We here address this issue using both qRT-PCR and in situ hybridization
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Transcription of the WUSCHEL-RELATED HOMEOBOX 4 gene in Arabidopsis thaliana Gene Expr. Patterns (IF 0.897) Pub Date : 2020-10-14 Satish Kumar Eeda; Wolfgang Werr
Phylogenetic shadowing and chromatin accessibility data suggested that essential regulatory elements are absent in the 2.9 kb immediate upstream region of the published WOX4pro::YFP cambium marker. Inclusion of an additional 6.3 kb of upstream promoter sequence and confocal imaging with different fluorophores in transgenic Arabidopsis lines revealed a much wider cell-type-specific expression pattern
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The mesenchymal property of mouse mammary anlagen repopulating cell population is associated with its stemness Gene Expr. Patterns (IF 0.897) Pub Date : 2020-10-22 Jiazhe Song; Wenyong Ding; Yinhui Liu; Lin Lin; Meng Jia; Shujun Liu; Kai Xue
During early embryogenesis, mammary glands are derived from surface ectoderm and their morphogenesis is controlled by mammary stem cells (MaSCs) and epithelial-mesenchymal transition (EMT). Mammary anlagen stage (E13.5–15.5) is an important stage for fetal mice to achieve EMT dependent mammary morphogenesis. And the characteristics of mammary anlagen repopulating cell population (MaRC) should be identified
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Developmental expression patterns of six6: A gene linked with spawning ecotypes in Atlantic salmon Gene Expr. Patterns (IF 0.897) Pub Date : 2020-09-30 Jacqueline Emmanuel Moustakas-Verho; Johanna Kurko; Andrew H. House; Jaakko Erkinaro; Paul Debes; Craig Robert Primmer
The Atlantic salmon has been studied extensively, particularly as a model for understanding the genetic and environmental contributions to the evolution and development of life history traits. Expression pattern analysis in situ, however, is mostly lacking in salmon. We examine the embryonic developmental expression of six6, a candidate gene previously identified to be associated with spawning ecotypes
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Molecular characterization and expression pattern of inhibin α and βb in Chinese tongue sole (Cynoglossus semilaevis) Gene Expr. Patterns (IF 0.897) Pub Date : 2020-09-24 Ning Zhang; Zhongdian Dong; Wenteng Xu; Zhongkai Cui; Qian Wang; Songlin Chen
Inhibin plays important roles in vertebrate reproduction and development. In this study, we have cloned two genes encoding inhibin subunits, inhα and ihnβb, in Chinese tongue sole. inhα consists of 1032 bp, encoding a 343 amino-acid protein. inhβb is composed of 1275 bp, encoding a 424 amino-acid protein. Phylogenetic tree analysis indicated that INHα and INHβB were independently evolved. qPCR showed
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Expression analysis of mammalian mitochondrial ribosomal protein genes Gene Expr. Patterns (IF 0.897) Pub Date : 2020-09-25 Agnes Cheong; Ranjana Lingutla; Jesse Mager
Mitochondrial ribosomal proteins (MRPs) are essential components for the structural and functional integrity of the mitoribosome complex. Throughout evolution, the mammalian mitoribosome has acquired new Mrp genes to compensate for loss of ribosomal RNA. More than 80 MRPs have been identified in mammals. Here we document expression pattern of 79 Mrp genes during mouse development and adult tissues
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Quantitative gene expression dynamics of key placode signalling factors in the embryonic chicken scleral ossicle system. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-08-02 Jennifer L Giffin,Tamara A Franz-Odendaal
Development of the scleral ossicles, a ring of bony elements within the sclera, is directed by a series of papillae that arise from placodes in the conjunctival epithelium over a 1.5-day induction period in the chicken embryo. The regular spacing of the papillae around the corneal-scleral limbus suggests that their induction may be regulated by a reaction-diffusion mechanism, similar to other epithelial
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Comparative sequence analysis across Brassicaceae, regulatory diversity in KCS5 and KCS6 homologs from Arabidopsis thaliana and Brassica juncea, and intronic fragment as a negative transcriptional regulator. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-09-16 Swati Singh,R Geeta,Sandip Das
Intra- and epicuticular-waxes primarily comprising of very long chain aliphatic lipid (VLCFA), terpenoids and secondary metabolites such as sterol and flavonoids played a major role in successful colonization of terrestrial ecosystem by aquatic plants and are thus considered as a key evolutionary innovation. The key rate limiting step of Fatty Acid (FA) biosynthesis of condensation/elongation are catalyzed
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Genome-wide analysis of the RGP gene family in Populus trichocarpa and their expression under nitrogen treatment. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-09-06 Xue Sun,Lina Cao,Shuang Zhang,Jiajie Yu,Xiuyue Xu,Caifeng Xu,Zhiru Xu,Chunpu Qu,Guanjun Liu
Reversible glycosylation polypeptide (RGP) is a type of plant-specific protein, primarily involved in the biosynthesis of cell wall polysaccharides, which in turn changes the shape of the cell walls and affects the wood properties of plants. Poplar is a major industrial timber species, and the RGP gene has not been studied. This study uses bioinformatics methods to predict physical and chemical characters
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The expression of zebrafish NAD(P)H:quinone oxidoreductase 1(nqo1) in adult organs and embryos. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-09-01 Min Liu,Jinping Guo,Bingyu Chen,Jiamin Xia,Xiaohua Pu,Xinyu Zou,Min Yang,Sizhou Huang
NQO1, NAD(P)H: quinone oxidoreductase 1, was first identified in rat and its role has been extensively studied. Even the roles of NQO1 in the maintenance of physiological function and disease were largely addressed, whether the tissue specific functions of the NQO1 in organ development remains unknown. In the current study, we identified two NQO1 isoforms (isoform 1 and isoform 2) and examined the
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The modular expression patterns of three pigmentation genes prefigure unique abdominal morphologies seen among three Drosophila species. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-08-21 William A Dion,Mujeeb O Shittu,Tessa E Steenwinkel,Komal K B Raja,Prajakta P Kokate,Thomas Werner
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Distinct expression patterns of seven crucial microRNAs during early embryonic development in medaka (Oryzias latipes). Gene Expr. Patterns (IF 0.897) Pub Date : 2020-08-13 Xuegeng Wang,Xiaohong Song,Ramji K Bhandari
MicroRNAs (i.e. miRNAs) are small non-coding RNAs that play essential modulation roles in embryonic development in vertebrates. Paternal and maternal miRNAs contribute to the development of post-fertilization embryo and zygotic genome activation. The pattern of expression and their roles in embryonic development of medaka are not clearly understood. The present study, therefore, examined a temporal
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Stage-specific expression patterns of ER stress-related molecules in mice molars: Implications for tooth development. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-08-03 Yam Prasad Aryal,Eui-Seon Lee,Tae-Young Kim,Shijin Sung,Ji-Youn Kim,Seo-Young An,Jae-Kwang Jung,Jung-Hong Ha,Jo-Young Suh,Hitoshi Yamamoto,Wern-Joo Sohn,Sung-Won Cho,Youngkyun Lee,Chang-Hyeon An,Jae-Young Kim
The endoplasmic reticulum (ER) is a site where protein folding and posttranslational modifications occur, but when unfolded or misfolded proteins accumulate in the ER lumen, an unfolded protein response (UPR) occurs. A UPR activates ER-stress signalling genes, including inositol-requiring enzyme-1 (Ire1), activating transcription factor 6 (Atf6), and double-stranded RNA-activated protein kinase-like
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Identification and functional analysis of the doublesex gene in the redclaw crayfish, Cherax quadricarinatus. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-07-24 Jianbo Zheng,Lina Cai,Yongyi Jia,Meili Chi,Shun Cheng,Shili Liu,Fei Li,Zhimi Gu
DM-domain (Zn-finger motif domain) genes play an important role in the sex determination and differentiation among animal kingdom. In the present study, the gene of Doublesex (Cqdsx) was identified and characterized for the first time in the redclaw crayfish, Cherax quadricarinatus. The full-length cDNA was 1271 bp, comprising a 155 bp 5′-untranslated region (5′-UTR), an 885 bp predicted open reading
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Myelopoiesis specific gene expression profiling in human CD34+ hematopoietic stem cells. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-07-22 Madhuri Joshi,Gurudutta Gangenahalli
Differentiation of the HSCs into myeloid lineage is primarily monitored by transcription factor PU.1. GATA1 acts as a negative regulator by antagonizing the function of PU.1 by bindings its β3/β4 domain. In this study, a mutation was induced in PU.1 which blocks its interaction with GATA1. The pure form of this mutant protein i.e Y244D was loaded on poly (lactic-co-glycolic acid) nanoparticles to transfect
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TWIST1 upregulates matrix metalloproteinase (MMP) genes family in esophageal squamous carcinoma cells. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-07-22 Sima Ardalan Khales,Mohammad Reza Abbaszadegan,Ahmad Majd,Mohammad Mahdi Forghanifard
Twist-related protein 1 (TWIST1), a highly conserved basic helix-loop-helix transcription factor, stimulates epithelial-mesenchymal transition (EMT) and plays a crucial role in the regulation of the extracellular matrix (ECM) and cell-cell adhesion. Our aim in this study was to evaluate the functional correlation between TWIST1 and MMP genes in human ESCC cell lines, KYSE-30 and YM-1. To generate recombinant
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Expression patterns of activating transcription factor 5 (atf5a and atf5b) in zebrafish. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-07-11 Roberto Rodríguez-Morales,Viveca Vélez-Negrón,Aranza Torrado-Tapias,Gaurav Varshney,Martine Behra
The Activating Transcription Factor 5 (ATF5) is a basic leucine-zipper (bZIP) transcription factor (TF) with proposed stress-protective, anti-apoptotic and oncogenic roles which were all established in cell systems. In whole animals, Atf5 function seems highly context dependent. Atf5 is strongly expressed in the rodent nose and mice knockout (KO) pups have defective olfactory sensory neurons (OSNs)
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On the origin of vertebrate body plan: Insights from the endoderm using the hourglass model. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-06-27 Chunpeng He,Tingyu Han,Xin Liao,Rui Guan,J-Y Chen,Kimberly D Tremblay,Zuhong Lu
The vertebrate body plan is thought to be derived during the early Cambrian from a worm-like chordate ancestor. While all three germ layers were clearly involved in this innovation, the role of the endoderm remains elusive. According to the hourglass model, the optimal window for investigating the evolution of vertebrate endoderm-derived structures during cephalochordate development is from the Spemann's
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DOF, MYB and TCP transcription factors: Their possible roles on barley germination and seedling establishment. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-06-27 Merve Seven,Hulya Akdemir
Seed germination is a multi-staged complex process during seed plant life cycle, and it is tightly regulated through a coordinated expression of diverse genes in diverse tissues. As regulatory molecules of gene expression, determination of transcription factors is crucial to understanding molecular basis and regulatory network of germination process and seedling establishment. However, limited data
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Overexpression of a sour jujube gene ZjPYR1, encoding a putative abscisic acid receptor, increases sensitivity of the stomata and roots to ABA in Arabidopsis thaliana. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-05-22 Lanting Qi,Yuguang Zheng,Peiyan Wang,Junna Song,Songsong Jing,Lijun Xu,Xuanyu Zhou,Zongqian Hao,Yuping Yan,Zhao Liu
The abscisic acid (ABA) receptor binds to ABA in plants and can activate the ABA signaling pathway to initiate stress resistance. Sour jujube (Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. Chou) is an economic tree crop in North China, which is strongly adapted to drought and salt stress. Here, we cloned and overexpressed the ZjPYR1 gene in Arabidopsis thaliana. After ABA treatment, the accumulation
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Expression of three P4-phospholipid flippases-atp11a, atp11b, and atp11c in zebrafish (Danio rerio). Gene Expr. Patterns (IF 0.897) Pub Date : 2020-04-25 Alexia Hawkey-Noble,Jurgienne Umali,Gerissa Fowler,Curtis R French
Cellular membrane asymmetry is a hallmark characteristic of all eukaryotic cells. The balance of phospholipid composition within the cytoplasmic inner leaflet and the extracellular outer leaflet of the plasma membrane (PM) maintains cellular function and vitality. The proper exposure of particular phospholipids is necessary to maintain cellular signalling, controlled apoptosis, and vesicle transportation
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Expression of zebrafish cpsf6 in embryogenesis and role of protein domains on subcellular localization. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-04-21 Carla M Borini Etichetti,Albano Tenaglia,María Nicol Arroyo,Javier E Girardini
CPSF6 is a component of the CFIm complex, involved in mRNA 3′end processing. Despite increasing interest on this protein as a consequence of proposed roles in cancer and HIV infection, several aspects of CPSF6 biological function are poorly understood. In this work we studied the expression of the zebrafish ortholog cpsf6 in early stages of embryo development. Quantitative RT-PCR studies showed that
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Physiological and transcriptomic analysis of 'Whangkeumbae' pear core browning during low-temperature storage. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-04-20 Hongsheng Zhou,Mengyao Tian,Wen Huang,Shufen Luo,Huali Hu,Yingtong Zhang,Leigang Zhang,Pengxia Li
Core browning of ‘Whangkeumbae’ pear has become an urgent problem in the Chinese pear industry, which often appears after several months of low-temperature storage. However, little is known regarding the crosstalk between physiology and molecular mechanisms regulating the core browning process of the pear. In this study, the physiological and genetic responses of the core were identified during storage
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Molecular characterization and expression profiles of transcription factor Sox gene family in Culter alburnus. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-04-04 Jianbo Zheng,Yongyi Jia,Shili Liu,Meili Chi,Shun Cheng,Zhimi Gu
Sox protein family is characterized by the presence of the conserved high-mobility group (HMG) box. Sox transcription factors are involved in diverse developmental process in animals, including sex-determination, organogenesis, embryogenesis, neurogenesis, and cell fate decision. In this study, 23 Sox genes were identified based on the Culter alburnus whole-genome sequence and categorized into six
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The spatiotemporal expression pattern of Syndecans in murine embryonic teeth. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-03-24 Jingyi Wu,Hong Li,Lu Han,Tianyu Sun,Ye Tian,Xiaofang Wang
The hierarchical interactions between the dental epithelium and dental mesenchyme represent a common paradigm for organogenesis. During tooth development, various morphogens interact with extracellular components in the extracellular matrix and on the cell surfaces to transmit regulatory signaling into cells. We recently found pivotal roles of FAM20B-catalyzed proteoglycans in the control of murine
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Expression pattern of the small muscle protein, X-linked (smpx) gene during zebrafish embryonic and larval developmental stages. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-03-19 Anna Ghilardi,Alberto Diana,Laura Prosperi,Luca Del Giacco
The small muscle protein, X-linked (SMPX) gene encodes a cytoskeleton-associated protein, highly expressed in both cardiac and skeletal muscles, as well as in fetal inner ears, with suggested roles as mechanotransductor. Recently, several mutations in the SMPX gene have been associated with X-chromosomal progressive deafness in human. However, very little information is known concerning the roles of
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Distribution of transcripts of the GFOD gene family members gfod1 and gfod2 in the zebrafish central nervous system. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-03-18 Carina G Lechermeier,Andrea D'Orazio,Marcel Romanos,Christina Lillesaar,Carsten Drepper
The glucose-fructose oxidoreductase domain containing gene family (GFOD) is small and contains only two members in human (GFOD1 and GFOD2). Information about its function is scarce. As the name implies the proteins contain an enzyme-defining domain, however, if this is functional and has enzymatic activity remains to be shown. A single nucleotide polymorphism situated in an intron of GFOD1 was found
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Developmental expression of doublesex-related transcripts in the common house spider, Parasteatoda tepidariorum. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-02-24 Mindy Gruzin,Marina Mekheal,Kelsey Ruhlman,Madison Winkowski,Jessica Petko
Sex determination is a rapidly evolving biological process controlled by differential gene expression. One family of transcription factors that initiate sex-specific gene expression and differentiation in many animal species are the Doublesex and Mab-3 (DM) domain proteins. While much is known about Doublesex-related proteins in various insect orders and commonly studied model systems, little is known
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Analysis of zebrafish cryptochrome2 and 4 expression in UV cone photoreceptors. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-02-21 Spencer D Balay,Sonya A Widen,Andrew J Waskiewicz
Cryptochromes (Cry) are ancient flavoproteins known to regulate circadian rhythms. In plants and some animals, Cry is sensitive to blue light due to its ability to bind the chromophore flavin adenine dinucleotide (FAD). Cry is also suggested to function in magnetoreception, since it can create light-dependent radical pairs with FAD that are sensitive to magnetic fields (Ritz2000; Liedvogel et al.,
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Expression pattern of Nav2 in the murine CNS with development. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-02-18 Caitlin Pook,Jamie M Ahrens,Margaret Clagett-Dame
Neuron navigator 2 (NAV2, RAINB1, POMFIL2, HELAD1, unc53H2) is essential for nervous system development. In the present study the spatial distribution of Nav2 transcript in mouse CNS during embryonic, postnatal and adult life is examined. Because multiple NAV2 proteins are predicted based on alternate promoter usage and RNA splicing, in situ hybridization was performed using probes designed to the
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Expression of NFIA and NFIB within the murine spinal cord. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-02-15 Elise Matuzelski,Tracey J Harvey,Danyon Harkins,Thuan Nguyen,Marc J Ruitenberg,Michael Piper
The Nuclear factor I proteins comprise a family of transcription factors that are expressed in many developing and mature cell populations, including within the central nervous system. Within the embryonic mouse spinal cord, NFIA and NFIB are expressed by neural progenitor cells lining the central canal, where they act to promote astrocytic and oligodendrocytic lineage specification. Cells lining the
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Detailed process analysis for glomerular capillary formation by immunofluorescence on ultra-thick sections. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-02-03 Ting Yu,Fang Zhang,Yan Wu,Jianli Chen,Lu Dai,Furong Li,Xiaoliang Liu,Chi Liu,Jinghong Zhao
Glomerular capillary formation is one of the fundamental mysteries in renal developmental biology. However, there are still debates on this issue, and its detailed formation process has not been clarified. To resolve this problem, we performed antibody staining with ultra-thick section on embryonic and postnatal mouse kidneys. We obtained the expression patterns of several genes that play an important
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Expression patterns of pcbp gene family members during zebrafish embryogenesis. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-01-31 Jie Wang,Xiaoling Guo,Dongxian Wang,Shulan Yang
The poly(C)-binding protein (PCBP) family members belong to a subtype of RNA-binding proteins that are ubiquitously expressed with diverse functions. In mammals, PCBP family, also known as hnRNP E family, is composed of four proteins, namely PCBP1, PCBP2, PCBP3 and PCBP4. So far, no study has been documented on the physiological roles of each member in vertebrate development. Here we analysed the spatiotemporal
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Identification and characterization of germ cell genes vasa and dazl in a protogynous hermaphrodite fish, orange-spotted grouper (Epinephelus coioides). Gene Expr. Patterns (IF 0.897) Pub Date : 2020-01-30 Ling Qu,Xi Wu,Meifeng Liu,Chaoyue Zhong,Hongyan Xu,Shuisheng Li,Haoran Lin,Xiaochun Liu
Vasa and dazl genes have been reported to play pivotal roles in germ cell development and differentiation both in vertebrates and invertebrates; however, little is known about their functions in germ cell differentiation during gametogenesis and sex reversal in hermaphroditic fish. In the present study, vasa (Ecvasa) and dazl (Ecdazl) cDNA were cloned from orange-spotted grouper (Epinephelus coioides)
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Expression of MYOSIN VIIA in developing mouse cochleovestibular ganglion neurons. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-01-07 Sarah Law,Molly Stout,Amanda Rensch,Jennifer M Rowsell
Myosins make up a large super family of motor proteins responsible for actin-based motility in most eukaryotic cells. Myosin VIIA is essential for the development and function of sensory hair cells in the inner ear. The role of Myosin VIIA in the development of cochleovestibular ganglion (CVG) neurons in the mouse is largely unknown. Neurons of the CVG innervate sensory hair cells of the cochlea and
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A new member of the forkhead box protein family in zebrafish: Domain composition, phylogenetic implication and embryonic expression pattern. Gene Expr. Patterns (IF 0.897) Pub Date : 2020-01-07 Chih-Wei Zeng,Jin-Chuan Sheu,Huai-Jen Tsai
In this study, we reported a novel member of Forkhead box (Fox) proteins found in model zebrafish (Danio rerio). This new gene we cloned was primarily assigned as zgc:162612, which locates on Chromosome 3 at 26,108,033–26,109,322 in the zebrafish genome, but encodes an uncharacterized protein, LOC100037333. After we determined the nucleotide and deduced amino acid sequences of zgc:162612, we found
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Dynamic Delta-like1 expression in presomitic mesoderm cells during somite segmentation Gene Expr. Patterns (IF 0.897) Pub Date : 2019-12-31 Akari Takagi, Akihiro Isomura, Kumiko Yoshioka-Kobayashi, Ryoichiro Kageyama
During somite segmentation, the expression of clock genes such as Hes7 oscillates synchronously in the presomitic mesoderm (PSM). This synchronous oscillation slows down in the anterior PSM, leading to wave-like propagating patterns from the posterior to anterior PSM. Such dynamic expression depends on Notch signaling and is critical for somite formation. However, it remains to be determined how slowing
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Differential gene and protein expression in gastrocnemius and tibialis anterior muscle following tibial and peroneal nerve injury in rats Gene Expr. Patterns (IF 0.897) Pub Date : 2019-12-04 Yaofa Lin, Zheng Xie, Jun Zhou, Gang Yin, Haodong Lin
Peripheral nerve injury is encountered quite commonly in the clinic, and treatment results are often not satisfactory. Therefore, promoting nerve regeneration and functional recovery is a primary goal of neuroscience research. Recovery of corresponding target muscle can differ following peripheral nerve injury, but the reasons are unknown. Herein, we investigated differential gene and protein expression
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Differential expression of foxo genes during embryonic development and in adult tissues of Xenopus tropicalis. Gene Expr. Patterns (IF 0.897) Pub Date : 2019-11-23 Li Zheng,Cheng-Zhou Mao,Yun-Qian Bi,Yi-Min Zhou,Zhou Zhang,Hui Zhao,Kyu-Sang Park,Ruijin Huang,Dong-Qing Cai,Xu-Feng Qi
The forkhead-box transcription factors of O subfamily (FOXO) play important roles in regulation of various biological functions. We cloned foxo1, foxo3, foxo4, and foxo6 from Xenopus tropicalis (hereafter X. tropicalis), and examined their expression in embryos and adult tissues. Maternal transcripts of foxo1 and foxo3 genes are detected within the animal half of the early embryo, their zygotic transcripts
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Expression patterns of three JAK-STAT pathway genes in feather follicle development during chicken embryogenesis. Gene Expr. Patterns (IF 0.897) Pub Date : 2019-11-20 Yingfeng Tao,Xiaoliu Zhou,Zhiwei Liu,Xiaokang Zhang,Yangfan Nie,Xinting Zheng,Shaomei Li,Xuewen Hu,Ge Yang,Qianqian Zhao,Chunyan Mou
The Janus kinase (JAK)–signal transducer and activator of transcription (STAT) (JAK–STAT) pathway is shown to restrain the hair follicles in catagen and telogen and prevent anagen reentry in murine hair follicle cycling. The early roles of JAK-STAT pathway genes in skin development remain uncharacterized in mouse and chicken models. Here, we revealed the expression patterns of three JAK-STAT pathway
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GTP binding protein 10 is a member of the OBG family of proteins and is differentially expressed in the early Xenopus embryo. Gene Expr. Patterns (IF 0.897) Pub Date : 2019-03-05 Razhan Jerry,Jessica Sullivan-Brown,Michael D Yoder
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Sox9 in mouse urogenital sinus epithelium mediates elongation of prostatic buds and expression of genes involved in epithelial cell migration. Gene Expr. Patterns (IF 0.897) Pub Date : 2019-10-25 Andrew J Schneider,Joseph Gawdzik,Chad M Vezina,Tracie R Baker,Richard E Peterson
Previous studies identified Sox9 as a critical mediator of prostate development but the precise stage when Sox9 acts had not been determined. A genetic approach was used to delete Sox9 from mouse urogenital sinus epithelium (UGE) prior to prostate specification. All prostatic bud types (anterior, dorsolateral and ventral) were stunted in Sox9 conditional knockouts (cKOs) even though the number of prostatic
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Expression of nucleosome assembly protein 1 like genes in zebrafish embryos. Gene Expr. Patterns (IF 0.897) Pub Date : 2019-10-24 Shuna Sun,Xuan Li,Ziyin Liu,Guoping Zhang,Chunjie Yang,Qiu Jiang,Yunzeng Zou
Nucleosome assembly protein 1-like (Nap1l) family plays numerous biological roles including nucleosome assembly, transcriptional regulation, and cell cycle progression. However, the tissue specific in vivo functions of the Nap1l family members remain largely unknown. In this study, we finished the complete expression patterns of nap1l1 and nap1l4a in zebrafish embryos by whole-mount in situ hybridization
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Armadillo-like helical domain containing-4 is dynamically expressed in both the first and second heart fields. Gene Expr. Patterns (IF 0.897) Pub Date : 2019-10-24 Simon J Conway,Reagan McConnell,Olga Simmons,Paige L Snider
Armadillo repeat and Armadillo-like helical domain containing proteins form a large family with diverse and fundamental functions in many eukaryotes. Herein we investigated the spatiotemporal expression pattern of Armadillo-like helical domain containing 4 (or Armh4) as an uncharacterized protein coding mouse gene, within the mouse embryo during the initial stages of heart morphogenesis. We found Armh4
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The expression of FoxG1 in the early development of the European river lamprey Lampetra fluviatilis demonstrates significant heterochrony with that in other vertebrates. Gene Expr. Patterns (IF 0.897) Pub Date : 2019-09-28 Galina V Ermakova,Alexandr V Kucheryavyy,Andrey G Zaraisky,Andrey V Bayramov
FoxG1, a member of the Fox/Forkhead family of winged helix transcription factors, plays key roles in the induction and spatial compartmentalization of the telencephalon in vertebrates. Loss- and gain-of-function experiments have established FoxG1 as a maintenance factor for neural progenitors and a crucial player in the specification of the ventral telencephalon (subpallium). For the first time in
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Novel cis-regulatory regions in ecdysone responsive genes are sufficient to promote gene expression in Drosophila ovarian cells. Gene Expr. Patterns (IF 0.897) Pub Date : 2019-09-26 Samantha I McDonald,Allison N Beachum,Taylor D Hinnant,Amelia J Blake,Tierra Bynum,E Parris Hickman,Joseph Barnes,Kaely L Churchill,Tamesia S Roberts,Denise E Zangwill,Elizabeth T Ables
The insect steroid hormone ecdysone is a key regulator of oogenesis in Drosophila melanogaster and many other species. Despite the diversity of cellular functions of ecdysone in oogenesis, the molecular regulation of most ecdysone-responsive genes in ovarian cells remains largely unexplored. We performed a functional screen using the UAS/Gal4 system to identify non-coding cis-regulatory elements within
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Selection and validation of castor bean (Ricinus communis) reference genes for quantitative PCR (RT-qPCR) in developing and germinating seeds and expression pattern of four ricin-family genes. Gene Expr. Patterns (IF 0.897) Pub Date : 2019-09-16 Antônio José Rocha,Mario Ramos de Oliveira Barsottini,Soraya Lília da Rocha
This study aims to expand the set of internal control genes used for RT-qPCR experiments with Castor bean (Ricinus communis) seeds by evaluating candidate genes across several seed tissues and developmental stages. Nine reference genes were selected, including actin-11 (ACT11), tubulin alpha-2 (Tα2), elongation factor 1-alpha (EF1-α), protein phosphatase 2A-2 (PP2A2), polyubiquitin-3 (PUB3) and gl
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Ontogeny and tissue-specific expression of immune-relevant genes in Catla catla (Hamilton). Gene Expr. Patterns (IF 0.897) Pub Date : 2019-09-14 P Arya,P K Pradhan,Anutosh Paria,R Sharma,Dev K Verma,Ravindra,G Rathore,N Sood
India is the second largest fish producing country in the world with production of 12.6 million tonnes (mt) in 2017–18, and Indian major carps (IMCs) contribute bulk of this fish production. Catla, Catla catla is the fastest growing species among IMCs. However, the survival rate of catla during larval rearing is normally lower than the other IMCs i.e rohu Labeo rohita, and mrigal Cirrhinus mrigala
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Comparative gene expression analysis of the engulfment and cell motility (ELMO) protein family in the mouse brain. Gene Expr. Patterns (IF 0.897) Pub Date : 2019-09-12 Yumi Sato,Akira Sato,Shota Mizuno,Jun-Na Hirota,Shuhei Fujima,Chiaki Ishii,Yoshitake Sano,Teiichi Furuichi
Engulfment and cell motility (ELMO) proteins bind to Dock180, a guanine nucleotide exchange factor (GEF) of the Rac family, and regulate GEF activity. The resultant ELMO/Dock180/Rac module regulates cytoskeletal reorganization responsible for the engulfment of apoptotic cells, cell migration, and neurite extension. The expression and function of Elmo family proteins in the nervous system, however,
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Spatiotemporal expression profile of embryonic and adult ankyrin repeat and EF-hand domain containing protein 1-encoding genes ankef1a and ankef1b in zebrafish. Gene Expr. Patterns (IF 0.897) Pub Date : 2019-09-12 Jeffrey G Daniel,Jennifer R Panizzi
Recent human next-generation sequencing (NGS) studies indicate a correlation between ANKEF1 (ankyrin repeat and EF-hand domain containing protein 1) expression and cilia formation or function. Additionally, a single study conducted in the African clawed frog (Xenopus laevis) showed ankef1 is down-regulated after pharmacological fibroblast growth factor (FGF) inhibition and plays a role in protocadherin-mediated