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Identification of Potential Biomarkers of Septic Shock Based on Pathway and Transcriptome Analyses of Immune-Related Genes Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Jie Wang, Jie Cai, Linlin Yue, Xixi Zhou, Chunlin Hu, Hongquan Zhu, Ramachandran Vasudevan
Immunoregulation is crucial to septic shock (SS) but has not been clearly explained. Our aim was to explore potential biomarkers for SS by pathway and transcriptional analyses of immune-related genes to improve early detection. GSE57065 and GSE95233 microarray data were used to screen differentially expressed genes (DEGs) in SS. Gene Ontology and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway
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Characterization and Prognosis of Biological Microenvironment in Lung Adenocarcinoma through a Disulfidptosis-Related lncRNAs Signature Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Zhuo Yang, Shenglan Cao, Fangli Wang, Kangming Du, Fang Hu, Hongda Liu
Background. The role of disulfidptosis-related lncRNAs remains unclear in lung adenocarcinoma. Methods. Analysis in R software was conducted using different R packages, which are based on the public data from The Cancer Genome Atlas (TCGA) database. The transwell assay was used to evaluate the invasion and migration abilities of lung cancer cells. Results. In our study, we identified 1401 lncRNAs significantly
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Identification of DUSP7 as an RNA Marker for Prognostic Stratification in Acute Myeloid Leukemia: Evidence from Large Population Cohorts Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Xin Gao, Hongda Liu
Background. The problem of prognostic stratification in acute myeloid leukemia (AML) patients still has limitations. Methods. The expression profile data and clinical features of AML patients were obtained from multiple publicly available sources, including GSE71014, TCGA-LAML, and TARGET-AML. Single-cell analysis was performed using the TISCH project. All the analysis was conducted in the R software
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Advanced Glycation End Products’ Receptor DNA Methylation Associated with Immune Infiltration and Prognosis of Lung Adenocarcinoma and Lung Squamous Cell Carcinoma Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Jun Yang, Mingqiang Lin, Mengyan Zhang, Zhiping Wang, Hancui Lin, Yilin Yu, Qunhao Zheng, Xiaohui Chen, Yahua Wu, Qiwei Yao, Jiancheng Li, Kanhaiya Singh
Background. Advanced glycation end products’ receptor (AGER) is a multiligand receptor that interacts with a wide range of ligands. Previous studies have shown that abnormal AGER expression is closely related to immune infiltration and tumorigenesis. However, the AGER DNA methylation relationship between prognosis and infiltrating immune cells in LUAD and LUSC is still unclear. Methods. AGER expression
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Identification of PANoptosis-Based Prognostic Signature for Predicting Efficacy of Immunotherapy and Chemotherapy in Hepatocellular Carcinoma Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Xiaofeng Xiong, Qianben Song, Mengjia Jing, Wei Yan, Hongda Liu
Background. PANoptosis has been a research hotspot, but the role of PANoptosis in hepatocellular carcinoma (HCC) remains widely unknown. Drug resistance and low response rate are the main limitations of chemotherapy and immunotherapy in HCC. Thus, construction of a prognostic signature to predict prognosis and recognize ideal patients for corresponding chemotherapy and immunotherapy is necessary. Method
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Bioinformatics-Based Identification of CircRNA-MicroRNA-mRNA Network for Calcific Aortic Valve Disease Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Linghong Song, Yubing Wang, Yufei Feng, Hao Peng, Chengyan Wang, Juncang Duan, Kejian Liu, Xihua Shen, Wenyi Gu, Yan Qi, Shan Jin, Lijuan Pang, Abd El-Latif Hesham
Background. Calcific aortic valve disease (CAVD) is the most common native valve disease. Valvular interstitial cell (VIC) osteogenic differentiation and valvular endothelial cell (VEC) dysfunction are key steps in CAVD progression. Circular RNA (circRNAs) is involved in regulating osteogenic differentiation with mesenchymal cells and is associated with multiple disease progression, but the function
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Systematically Prognostic Analyses of Gastric Cancer Patients with Ovarian Metastasis Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Peng Peng, Xiuyuan Liu, Lin Yang, Zhenguang Gu, Lin Cai, Hongda Liu
Ovarian metastasis of gastric cancer indicates that the disease has reached the late stage and the opportunity for radical surgery is restricted. However, the clinical characteristics and prognosis of patients with gastric cancer ovarian metastasis (GCOM) remain to be illustrated. Here, we retrieved the information of 780 GCOM cases from the Surveillance, Epidemiology, and End Results (SEERs) database
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Regulation of Hepatocytes in G0 and G1 Phases by NOTCH3 mRNA, miR-369-3p, and rno-Rmdn2_0006 during the Initial Stage of Rat Liver Regeneration Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Xiayan Zang, Zihui Wang, Yafei Li, Han Gao, Jianlin Guo, Wei Jin, Cuifang Chang, Juntang Lin, Kuicheng Zhu, Cunshuan Xu, Hongda Liu
The key event of liver regeneration initiation (LRI) is the switch of hepatocytes from the G0 phase to the G1 phase. This study aimed to use the data from large-scale quantitatively detecting and analyzing (LQDA) to reveal the regulation of hepatocytes in the G0 or G1 phase by competing endogenous RNAs (ceRNAs) during LRI. The hepatocytes of the rat liver right lobe were isolated 0, 6, and 24 h after
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CSRP1 Promotes Colon Adenocarcinoma Growth and Serves as an Independent Risk Biomarker for Worse Prognosis Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Senlong Yu, Haifeng Zhao, Hongjie Meng, Shengguang Shi, Shenghui Cao, Tianhua Bian, Canping Ruan, Xiaoye Jin
Background. Cysteine and Glycine Rich Protein 1 (CSRP1) belongs to the cysteine-rich protein family, which contains a unique double-zinc finger motif and is important for development and cellular differentiation. Abnormal expression of CSRP1 was reported within several malignancies such as prostate cancer and acute myeloid leukemia. Here, we explored function of CSRP1 within colon adenocarcinoma (COAD)
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Immunotherapy and Immune Infiltration in Patients with Clear Cell Renal Cell Carcinoma: A Comprehensive Analysis Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Lin Hou, Xinyue Liu, Hongda Liu
On a global scale, renal cell carcinoma (RCC) is the second most common form of cancer and the 10th leading cause of cancer-related deaths. There are about 70% of cases of RCC that are clear cell renal cell carcinomas (ccRCCs). This study explores possible targets for immune therapy in patients with RCC. In the recent years, immunotherapy has been applied to RCC patients. In order to identify genes
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Novel KDM2B/SAV1 Signaling Pathway Promotes the Progression of Gastric Cancer Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Ning Li, Haifei Song, Zhiqin Chen, Chen Chen, Ming Quan, Yaying Sun
Salvador homologue 1 (SAV1), which is reported to act as a tumor suppressor in different types of cancer, is one of the key components of the Hippo pathway. However, the expression and mechanisms of SAV1 in the development and progression of gastric cancer (GC) remain to be elucidated. Immunohistochemistry (IHC) was performed in the present study to assess the expression levels of SAV1 and lysine-specific
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Integrated Bioinformatics Analysis to Screen Hub Gene Signatures for Fetal Growth Restriction Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Jingjin Yang, Yuxin Liu, Minyue Dong, Ying-Kun Xu
Background. Fetal growth restriction (FGR) is the impairment of the biological growth potential of the fetus and often leads to adverse pregnancy outcomes. The molecular mechanisms for the development of FGR, however, are still unclear. The purpose of this study is to identify critical genes associated with FGR through an integrated bioinformatics approach and explore the potential pathogenesis of
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RNA Sequencing Reveals the Differentially Expressed circRNAs between Stable and Unstable Carotid Atherosclerotic Plaques Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Xueguang Lin, Ying Deng, Lujuan Ye, Bo Chen, Jindong Tong, Weijun Shi, Bo Wang, Bo Yu, Jingdong Tang, Hongda Liu
Objective. This study aimed to identify circular RNA profiles (circRNAs) via high-throughput RNA sequencing and distinguish the differentially expressed (DE) circRNAs between stable and unstable plaques. Methods. RNA sequencing was performed on unstable and stable carotid plaque samples obtained from patients with carotid artery stenosis. DE circRNAs were screened, and six DE circRNAs were verified
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The Progress of Research on Genetic Factors of Recurrent Pregnancy Loss Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Qinlan Li, Shuting Chen, Xinyi Dong, Sen Fu, Tianyu Zhang, Weiwei Zheng, Yonghong Tian, Donghui Huang, Nadeem Sheikh
Recurrent pregnancy loss (RPL) is both mental and physical health problem affecting about 1–5% of women of childbearing age. The etiology of RPL is complex, involving chromosomal abnormalities, autoimmune diseases, metabolic disorders, and endometrial dysfunction. The causes of abortion are still unknown in more than 50% of these cases. With the development of science and technology, an increasing
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Functional Analysis of Monkeypox and Interrelationship between Monkeypox and COVID-19 by Bioinformatic Analysis Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Eun Jung Sohn, John Charles Rotondo
The outbreak of monkeypox may be considered a novel and urgent threat after the coronavirus disease (COVID-19). No wide-ranging studies have been conducted on this disease since it was first reported. We systematically assessed the functional role of gene expression in cells infected with the monkeypox virus using transcriptome profiling and compared the functional relation with that of COVID-19. Based
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Clinical Significance of NKD Inhibitor of WNT Signaling Pathway 1 (NKD1) in Glioblastoma Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Lijun Li, Ruiying Gao, Weizhong Huangfu, Fang Zhang, Ruixia Wang, Hongda Liu
Introduction. As the most malignant type of gliomas, glioblastoma is characterized with disappointing prognosis. Here, we aimed to investigate expression and function of NKD inhibitor of Wnt signaling pathway 1 (NKD1), an antagonist of Wnt-beta-catenin signaling pathways, in glioblastoma. Methods. The mRNA level of NKD1 was firstly retrieved from TCGA glioma dataset to evaluate its correlation with
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The Identification of Immune-Related Biomarkers for Osteoarthritis Immunotherapy Based on Single-Cell RNA Sequencing Analysis Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Zhe Tan, Rong Chen, Hanyu Lin, Hong Wang, Hongda Liu
Osteoarthritis (OA) is a chronic musculoskeletal disease affecting approximately 500 million people worldwide. Globally, OA is one of the most common and leading causes of disability. Several genetic factors are involved in OA, including inherited genes, genetic susceptibility, and genetic predisposition. As the pathogenesis of OA is unknown, there are almost no effective treatments available to prevent
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Sex-Biased Expression of Genes Allocated in the Autosomal Chromosomes: Blood LC-MS/MS Protein Profiling in Healthy Subjects Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Hayder A. Giha, Rabab A. Abdulwahab, Jaafar Abbas, Zakia Shinwari, Ayodele Alaiya, John Charles Rotondo
Background. Sex and gender have a large impact in human health and disease prediction. According to genomic/genetics, men differ from women by a limited number of genes in Y chromosome, while the phenotypes of the 2 sexes differ markedly. Methods. In this study, serum samples from six healthy Bahraini men and women were analyzed by liquid chromatography–mass spectrometry (LC-MS/MS). Bioinformatics
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Comprehensive Analysis Based on the Cancer Immunotherapy and Immune Activation of Gastric Cancer Patients Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Feng Jiang, Qilong Ma, Hongda Liu
When it comes to aggressiveness and prognosis, immune cells play an important role in the microenvironment of gastric cancer (GC). Currently, there is no well-established evidence that immune status typing is reliable as a prognostic tool for gastric cancer. This study aimed to develop a genetic signature based on immune status typing for the stratification of gastric cancer risk. TCGA data were used
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miR-19-3p Targets PTEN to Regulate Cervical Cancer Cell Proliferation, Invasion, and Autophagy Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Wei Wang, Lu Liu, Yongjian Tian, John Charles Rotondo
Background. Cervical cancer is the second most common cancer among women worldwide. Extensive studies have shown that microRNAs (miRNA/miR) can regulate the formation, progression, and metastasis of cancer. The purpose of this study was to investigate the effect of miR-19-3p on the proliferation, invasion, and autophagy of cervical cancer cells and to explore the underlying mechanism. Methods. SiHa
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LncRNA MBNL1-AS1 Suppresses Cell Proliferation and Metastasis of Pancreatic Adenocarcinoma through Targeting Carcinogenic miR-301b-3p Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Chouman Sulidankazha, Alidake, Hai Lin, Tieying He, Wei Han, Qilong Chen, Hongda Liu
Pancreatic adenocarcinoma (PAAD) has been a huge challenge to public health due to its increasing incidence, frequent early metastasis, and poor outcome. The molecular basis of tumorigenesis and metastasis in PAAD is largely unclear. Here, we identified a novel tumor-suppressor long noncoding RNA (lncRNA) MBNL1-AS1, in PAAD and revealed its downstream mechanism. Quantitative real-time PCR (qRT-PCR)
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A Survival Model Based on the ASB Genes and Used to Predict the Prognosis of Kidney Renal Clear Cell Carcinoma Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Deqian Xie, Lu Dai, Xiaolei Yang, Tao Huang, Wei Zheng, Ningke Ruan
Kidney renal clear cell carcinoma (KIRC) is increasing in incidence worldwide, with poor and unpredictable patient prognosis limited by diagnostic and therapeutic approaches. New genes are urgently needed to improve this situation. The ankyrin repeat and suppressor of the cytokine signaling (SOCS) box (ASB) family are a promising class of tumorigenesis-related genes. We examined the expression and
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Association of Androgen-Receptor Gene Mutations with the Copy Number of Androgen-Receptor Silk Protein A Complex and Glutathione-S-Transferases T1 and M1 in Prostate Cancer Patients Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Yan Zhang, Xiangdi Meng, Zhaosen Ma, Zhou Sun, Zhixin Wang, Vasudevan Ramachandran
Objective. The purpose of our work was to explore the association of mutations in the androgen receptor gene and copy numbers of the androgen-receptor silk protein A complex with glutathione-S-transferases T1 and M1 in prostate cancer patients. Materials and Methods. Eighty-five patients with PC and 85 healthy controls were included in the study. Fasting peripheral venous blood was collected, whole
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Establishment of a Lymph Node Metastasis-Associated Prognostic Signature for Lung Adenocarcinoma Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Jiao Yu, Gang Li, Yingxuan Tian, Shufen Huo, Muhammad Babar Khawar
Background. Lung adenocarcinoma (LUAD) is the most common histological subtype of non-small cell lung cancer (NSCLC) with a low 5-year survival rate, which may be associated with the presence of metastatic tumors at the time of diagnosis, especially lymph node metastasis (LNM). This study aimed to construct a LNM-related gene signature for predicting the prognosis of patients with LUAD. Methods. RNA
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Exploration of the Long Noncoding RNAs Involved in the Crosstalk between M2 Macrophages and Tumor Metabolism in Lung Cancer Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Fang Fang, Yuanshan Yao, Zhe Ma, Hongda Liu
Background. Complex regulation exists between tumor metabolism and M2 macrophages. Long noncoding RNAs (lncRNAs) are famous for their wide regulatory role. This study aimed to identify the lncRNAs involved in the crosstalk between tumor metabolism and M2 macrophages. Methods. The Cancer Genome Atlas was responsible for the public data. R software was responsible for the analysis of public data. Results
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Molecular Targets of Shenqi Dihuang, A Traditional Chinese Herbal Medicine, and Its Potential Role in Renal Cell Carcinoma Therapy Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Xinglin Chen, Tongtong Zhang, Xiangyang Zhan, Xinyue Zang, Xinyu Zhai, Zhong Wan, Minyao Ge, Mingyue Tan, Jianyi Gu, Dongliang Xu, Hongda Liu
Chinese herbal medicine (CHM), which includes herbal slices and proprietary products, is widely used in China. Shenqi Dihuang (SQDH) is a traditional Chinese medicine (TCM) formula with ingredients that affect tumor growth. Despite recent advances in prognosis, patients with renal cell carcinoma (RCC) cannot currently receive curative treatment. The present study aimed to explore the potential target
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Lack of Association of C677T Methylenetetrahydrofolate Reductase Polymorphism with Breast Cancer Risk in Mali Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Brehima Diakite, Yaya Kassogue, Mamoudou Maiga, Guimogo Dolo, Oumar Kassogue, Jane L. Holl, Brian Joyce, Jun Wang, Kadidiatou Cisse, Fousseyni Diarra, Mamadou L. Keita, Cheick B. Traore, Bakarou Kamate, Sidi B. Sissoko, Bourama Coulibaly, Adama S. Sissoko, Drissa Traore, Fatoumata M. Sidibe, Sekou Bah, Ibrahim Teguete, Madani Ly, Sellama Nadifi, Hind Dehbi, Kyeezu Kim, Robert Murphy, Lifang Hou, Muhammad
Methylenetetrahydrofolate reductase (MTHFR) plays a major role in the metabolism of folates and homocysteine, which in turn can affect gene expression and ultimately promote the development of breast cancer. Thus, mutations in the MTHFR gene could influence homocysteine, methionine, and S-adenosylmethionine levels and, indirectly, nucleotide levels. Imbalance in methionine and S-adenosylmethionine
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Genetic Diversity and Population Structure of Selected Ethiopian Indigenous Cattle Breeds Using Microsatellite Markers Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Shelema Kelbessa Bora, Tesfaye Sisay Tessema, Gebrerufael Girmay, Xiaoye Jin
Background. In Ethiopia, livestock contributes 45% of agricultural GDP. Despite the economic role played by the sector, there have been little efforts to genetically improve the indigenous cattle. Morphological characterization of selected Ethiopian indigenous cattle has been made for (Bonga, Jimma, and Kerayu) cattle types. But, the selected indigenous cattle were not characterized at molecular level
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Identification of Key Biomarkers and Candidate Molecules in Non-Small-Cell Lung Cancer by Integrated Bioinformatics Analysis Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Liyan Yu, Xuemei Liang, Jianwei Wang, Guangxiang Ding, Jinhai Tang, Juan Xue, Xin He, Jingxuan Ge, Xianzhang Jin, Zhiyi Yang, Xianwei Li, Hehuan Yao, Hongtao Yin, Wu Liu, Shengchen Yin, Bing Sun, Junxiu Sheng, Ying Kun Xu
Background. Non-small cell lung cancer (NSCLC) is the most prevalent malignant tumor of the lung cancer, for which the molecular mechanisms remain unknown. In this study, we identified novel biomarkers associated with the pathogenesis of NSCLC aiming to provide new diagnostic and therapeutic approaches for NSCLC by bioinformatics analysis. Methods. From the Gene Expression Omnibus database, GSE118370
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Allelic and Genotype Frequencies of CYP2B6∗2 (64C > T) and CYP2B6∗3 (777C > A) in Three Dominant Ethnicities of the Iranian Population Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Armin Khavandegar, Bahareh Tavakoli-Far, Sarina Ansari, Parisa Veis-Karami, Faezeh Ghasemi, Samira Sheibaninia, Roshanak Jazayeri, Massoud Houshmand, Chiara Mazziotta
Background. Cytochrome P450 complex plays a key role in drug metabolism. CYP2B6 has an essential part in Cytochrome P450 complex metabolism. This study aims to determine the allelic distribution of CYP2B6∗2 and CYP2B6∗3 in three main Iranian ethnicities: Fars, Turk, and Kurd. Methods. The study was conducted on 174 unrelated healthy volunteers from three main Iranian ethnicities. After DNA extraction
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A Recurrent Nonsense Mutation in NECTIN4 Underlying Ectodermal Dysplasia-Syndactyly Syndrome with a Novel Phenotype in a Consanguineous Kashmiri Family Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Ghazanfar Ali, Sadia Sadia, Syeda Ain-ul- Batool, Zahid Azeem, Naheed Bashir Awan, Syed Akif Raza Kazmi, Zia- Ur- Rehman, Zeeshan Anjum, Fazal- Ur- Rehman, Abdul Wali, Kafaitullah Khan, Nasib Zaman, Muhammad Ayub, Muhammad Sajid, Noor Hassan, Saadullah Khan
EDSS1, a syndrome characterized by ectodermal dysplasia-syndactyly, is inherited in an autosomal recessive manner due to mutations in the NECTIN4/PVRL4 gene. Clinical manifestations of the syndrome include defective nail plate, sparse to absent scalp and body hair, spaced teeth with enamel hypoplasia, and bilateral cutaneous syndactyly in the fingers and toes. Here, we report a consanguineous family
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XRCC1 R194W and R399Q Polymorphisms and Colorectal Cancer Risk in a Northeastern Mexican Population Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Juan Pablo Meza-Espinoza, Valeria Peralta-Leal, Jorge Durán-González, Nelly Macías-Gómez, Anabel Bocanegra-Alonso, Evelia Leal-Ugarte, Xiaoye Jin
Colorectal cancer (CRC) is one of the most common cancers worldwide. Its etiopathogenesis is complex, mainly influenced by genetic instability caused by the accumulation of mutations. The XRCC1 gene, which is involved in DNA repair, has been associated with CRC through the R194W (C194T) and R399Q (G399A) polymorphisms, but the results are inconsistent. Here, we analyzed the association of these polymorphisms
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Orthodenticle Homeobox OTX1 Promotes Papillary Thyroid Carcinoma Progression and Is a Potential Prognostic Biomarker Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Jing Wei, Xin Wang, Kai Jiao, Hongda Liu
Papillary thyroid carcinoma (PTC) is the most common type of thyroid neoplasms, characterized by evidence of follicular cell differentiation. Orthodenticle homeobox 1 (OTX1) is a transcription factor which has been implicated in numerous diseases, including malignancies. The objective of this research was to explore the function of OTX1 in PTC. Immunohistochemistry (IHC) was employed to determine the
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Pharmacogenetic Approach for the Prevention of Rivaroxaban’s ADRs: A Systematic Review and Meta-Analysis Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Parham Mardi, Bahareh Abbasi, Arman Shafiee, Tara Afsharmoghaddam, Vasudevan Ramachandran
Introduction. Pharmacogenetics is a potential approach that can be applied to decline the burden of rivaroxaban’s ADRs. The current systematic review and meta-analysis aim to identify genetic variants correlated with rivaroxaban exposure and evaluate their importance. Methods. We systematically searched PubMed, Web of Science, and Scopus databases for all observational and interventional studies. The
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The Clinical Relevance and Functional Implications of Thymosin Beta-10 in Glioma Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Weimin Li, Jinliang Chen, Chengwei Xiang, Yong Long, Ke Wu, Juan Li, Hongda Liu
Glioma is a highly aggressive form of brain cancer characterized by limited treatment options and poor patient prognosis. In this study, we aimed to elucidate the oncogenic role of thymosin beta-10 (TMSB10) in glioma through comprehensive analyses of patient data from the TCGA and GTEx databases. Our investigation encompassed several key aspects, including the analysis of patients’ clinical characteristics
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Genetic Variations in the Human Angiotensin-Converting Enzyme 2 and Susceptibility to Coronavirus Disease-19 Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Taravat Talebi, Tannaz Masoumi, Katayoun Heshmatzad, Mahshid Hesami, Majid Maleki, Samira Kalayinia, Xiaoye Jin
Background. Health and economies are both affected by the coronavirus disease-19 (COVID-19) global pandemic. Angiotensin-converting enzyme 2 (ACE2) is a polymorphic enzyme that is a part of the renin-angiotensin system, and it plays a crucial role in viral entry. Previous investigations and studies revealed that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and ACE2 have a considerable
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The Clinical and Cellular Impact of RBMS2 on the Progression and Prognosis of Kidney Renal Clear Cell Carcinoma Genet. Res. (IF 1.5) Pub Date : 2024-01-01 Zhixiang Gao, Shouren Fan, Hongda Liu
This research delves into the implications of the RNA binding motif, single stranded interacting protein 2 (RBMS2)—a gene associated with tumor-suppressing functions—in the context of kidney renal clear cell carcinoma (ccRCC). Through meticulous exploration of online databases, we have identified a negative association between RBMS2 expression and adverse clinico-pathological features, such as advanced
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Assessment of Combined Karyotype Analysis and Chromosome Microarray Analysis in Prenatal Diagnosis: A Cohort Study of 3710 Pregnancies. Genet. Res. (IF 1.5) Pub Date : 2022-12-29 Jin Wang,Danni Wang,Yan Yin,Yi Deng,Mengling Ye,Ping Wei,Zhuo Zhang,Chun Chen,Shengfang Qin,Xueyan Wang
Objective The current study aimed to compare the characteristics of chromosome abnormalities detected by conventional G-banding karyotyping, chromosome microarray analysis (CMA), or fluorescence in situ hybridization (FISH)/CNVplex analysis and further explore the application value of combined karyotype analysis and CMA in prenatal diagnosis with a larger sample size. Methods From March 2019 to March
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Identification of the Immune Cell Infiltration Landscape in Head and Neck Squamous Cell Carcinoma (HNSC) for the Exploration of Immunotherapy and Prognosis. Genet. Res. (IF 1.5) Pub Date : 2022-12-28 Chunli Huang,Jifeng Liu
It is generally believed that the majority of head and neck cancers develop in the mucosal epithelial cells of the mouth, pharynx, and larynx, which is collectively known as head and neck squamous cell carcinoma (HNSC). As a complex pathological process, HNSC develops through a variety of cellular and molecular events. Cancerous cells and immune cells infiltrating tumors are the main components of
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Positive Expression of Retinol-Binding Protein 4 Is Related to the Malignant Clinical Features Leading to Poor Prognosis of Glioblastoma. Genet. Res. (IF 1.5) Pub Date : 2022-12-26 Xinqing Deng,Jian Ren,Zhongsheng Bi,Zhenghao Fu
Backgrounds Retinol-binding protein 4 (RBP4) is a monomeric-binding protein belonging to the lipocalin protein family, which has been reported to be dysregulated in several malignancies such as breast cancer and lung cancer. However, the expression and function of RBP4 in glioblastoma (GBM) are completely unknown. Materials and Methods TCGA datasets were used for analyzing the mRNA level of RBP4 in
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Bioinformatics Analysis of miRNAs Targeting TRAF5 in DLBCL Involving in NF-κB Signaling Pathway and Affecting the Apoptosis and Signal Transduction. Genet. Res. (IF 1.5) Pub Date : 2022-12-23 Chunyao Li,Lanshan Huang,Yongqin Wen,Muhua Yi,Min Gao
Background Diffuse large B-cell lymphoma (DLBCL) is an aggressive B-cell lymphoma with high heterogeneity. There is an unmet need to investigate valid indicators for the diagnosis and therapy of DLBCL. Methods GEO database was utilized to screen for differentially expressed genes (DEGs) and differential miRNAs in DLBCL tissues. The Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes
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OPN Promotes Cell Proliferation and Invasion through NF-κB in Human Esophageal Squamous Cell Carcinoma. Genet. Res. (IF 1.5) Pub Date : 2022-12-15 Bolin Chen,Shuzhi Liang,Haibin Guo,Li Xu,Jia Li,Jie Peng
Background Osteopontin (OPN) is a phosphorylated glycoprotein. There is increasing evidence that the OPN gene played a major role in the progression of solid organ tumors. However, few studies have clarified how OPN regulated the functional role of human esophageal squamous cell carcinoma (ESCC). This study was designed to investigate the effect of OPN in esophageal squamous cell carcinoma. Methods
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Analysis of Long Noncoding RNAs-Related Regulatory Mechanisms in Duchenne Muscular Dystrophy Using a Disease-Related lncRNA-mRNA Pathway Network. Genet. Res. (IF 1.5) Pub Date : 2022-12-14 Bing Xu,Chunlei Zheng
Objective This study aimed to investigate the molecular regulatory mechanisms underpinning Duchenne muscular dystrophy (DMD). Methods Using microarray data, differentially expressed long noncoding RNAs (DELs) and DMD-related differentially expressed mRNAs (DEMs) were screened based on the comparative toxicogenomics database, using a cutoff of |log2 fold change| > 1 and false discovery rate (FDR) < 0
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Cell Differentiation Trajectory Predicts Prognosis and Immunotherapeutic Response in Clear Cell Renal Cell Carcinoma. Genet. Res. (IF 1.5) Pub Date : 2022-11-29 Jin Xu,Xi Chen,Yinyu Chen,Qiushuang Wang,Yingliang Jin,Huashuo Zhao
Clear cell renal cell carcinoma (ccRCC) is the main type of malignancy in kidney related to glucose metabolism. Primary single cell culture and single cell sequencing are novel research technologies. In this study, we explored the differentiation status of ccRCC cells and its significance in prognosis and immunotherapeutic response through bioinformatics. We characterized distinct differentiation states
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Exploration of the Mechanism of Linoleic Acid Metabolism Dysregulation in Metabolic Syndrome. Genet. Res. (IF 1.5) Pub Date : 2022-11-28 Yan Wen,Yawen Shang,Qing Wang
We aimed to explore the mechanism of the linoleic acid metabolism in metabolic syndrome (MetS). RNA-seq data for 16 samples with or without MetS from the GSE145412 dataset were collected. Gene set variation analysis (GSVA), gene set enrichment analysis (GSEA), and gene differential expression analysis were performed. Expression data of differentially expressed genes (DEGs) involved in the linoleic
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LncRNA HOXA-AS2 Promotes Temozolomide Resistance in Glioblastoma by Regulated miR-302a-3p/IGF1 Axis. Genet. Res. (IF 1.5) Pub Date : 2022-11-21 Ligang Lin,Da Lin,Lingjiang Jin,Junyou Wang,Zheng Lin,Shuai Zhang,Gaojun Lin
Background Glioblastoma (GBM) is a highly prevalent brain tumor characterized by high rates of morbidity, recurrence, and mortality. While temozolomide (TMZ) is commonly used as a first-line treatment for this cancer, the emergence of TMZ resistance limits its utility. The long noncoding RNA HOXA-AS2 reportedly drives GBM progression, but whether it can influence therapeutic resistance to TMZ has yet
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Prognosis Signature of Cuprotosis-Related lncRNAs Associated with Kidney Renal Clear Cell Carcinoma. Genet. Res. (IF 1.5) Pub Date : 2022-11-17 Yiren Gao,Zhen Jia
Cuprotosis is a novel cell death mechanism that can be explored to treat various tumors. A few studies on the role of cuprotosis-related long noncoding RNA (lncRNA) in the development and prognosis of kidney renal clear cell carcinoma (KIRC) have been reported. We aimed to study the relationship between the prognosis of patients suffering from KIRC and lncRNAs associated with cuprotosis. The Cancer
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Triptolide Inhibits Th17 Response by Upregulating microRNA-204-5p and Suppressing STAT3 Phosphorylation in Psoriasis. Genet. Res. (IF 1.5) Pub Date : 2022-11-17 Qi He,Xingyue Wu,Quan Shi
Background Psoriasis is an immune and inflammation-related skin disease. Triptolide with immunosuppressive and anti-inflammatory properties has been utilized for psoriasis treatment. However, the potential immunological mechanisms of triptolide have not been fully elucidated. Methods Using an imiquimod (IMQ)-induced psoriatic mouse model, we detected the effects of triptolide on psoriasis-like lesions
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Upregulated Solute Carrier SLC39A1 Promotes Gastric Cancer Proliferation and Indicates Unfavorable Prognosis. Genet. Res. (IF 1.5) Pub Date : 2022-11-04 Dan Yu,Yong Chen,Ming Luo,Yanjin Peng,Shengen Yi
Backgrounds Solute carrier 39A1 (SLC39A1) is an indirect zinc transporter which showed diverse tumor-related functions in different malignancies. Here, we aimed to investigate its expression and role in gastric adenocarcinoma. Methods A retrospective gastric adenocarcinoma cohort (n = 154) was collected from our hospital to test their tissue expression of SLC39A1 through immunohistochemical staining
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Identification of Glucose Metabolism-Related Genes in the Progression from Nonalcoholic Fatty Liver Disease to Hepatocellular Carcinoma. Genet. Res. (IF 1.5) Pub Date : 2022-11-03 Siyuan Wang,Yiling Li,Ning Liu,Wei Shen,Wenhao Xu,Peng Yao
Nonalcoholic fatty liver disease (NAFLD) is a manifestation of hepatic metabolic syndrome that varies in severity. Hepatocellular carcinoma progresses from NAFLD when there is heterogeneity in the infiltration of immune cells and molecules. A precise molecular classification of NAFLD remains lacking, allowing further exploration of the link between NAFLD and hepatocellular carcinoma. In this work,
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Genome Survey and SSR Analysis of Camellia nitidissima Chi (Theaceae). Genet. Res. (IF 1.5) Pub Date : 2022-11-02 Yu Bai,Lin Ye,Kang Yang,Hui Wang
Camellia nitidissima Chi (CNC), a species of golden Camellia, is well known as "the queen of camellias." It is an ornamental, medicinal, and edible plant grown in China. In this study, we conducted a genome survey sequencing analysis and simple sequence repeat (SSR) identification of CNC using the Illumina sequencing platform. The 21-mer analysis predicted its genome size to be 2,778.82 Mb, with heterozygosity
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Inhibition of KCTD10 Affects Diabetic Retinopathy Progression by Reducing VEGF and Affecting Angiogenesis. Genet. Res. (IF 1.5) Pub Date : 2022-10-26 Yun Feng,Cong Wang,Guangwei Wang
Aim We purposed to evaluate the KCTD10 effects of angiogenesis in diabetic retinopathy (DR). Methods We induced a DR cell model using high glucose (HG) treatment of HRECs and ARPE-19 cells. A DR rat was established by injecting streptozotocin. Small interference RNA targeted KCTD10 (si-KCTD10) was used to mediate KCTD10 inhibition in cell and animal models. The roles of KCTD10 on cell viability, apoptosis
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Downregulation of hsa-miR-135b-5p Inhibits Cell Proliferation, Migration, and Invasion in Colon Adenocarcinoma. Genet. Res. (IF 1.5) Pub Date : 2022-10-21 Yunxin Zhang,Wentao Zhang,Wenlong Xia,Junwei Xia,Haishan Zhang
Colon cancer is the most common malignant tumor of the gastrointestinal tract, and approximately 80%-90% of colon cancers are colon adenocarcinomas (COADs). This study aimed to screen key microRNAs (miRNAs) associated with COAD. Differentially expressed (DE) miRNAs were screened between COAD and adjacent cancer samples based on the Gene Expression Omnibus (GEO) and the Cancer Genome Atlas obtained
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Relationship between CYP2C19 Polymorphism and Clopidogrel Resistance in Patients with Coronary Heart Disease and Ischemic Stroke in China. Genet. Res. (IF 1.5) Pub Date : 2022-10-21 Rong Chang,Wenqin Zhou,Yi Ye,Xiaofei Zhang,Yanmin Liu,Jinchun Wu
Objective Clopidogrel is widely used for preventing ischemic complications related to cardiovascular diseases. However, many patients experience clopidogrel resistance (CR). The polymorphisms of CYP2C19 have been implicated in CR, but CYP2C19 polymorphism considerably varies with both ethnic group and geographical location. This study aimed to investigate the association between CYP2C19 polymorphisms
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Upregulation of Klotho Aggravates Insulin Resistance in Gestational Diabetes Mellitus Trophoblast Cells. Genet. Res. (IF 1.5) Pub Date : 2022-10-21 Li Lin,Xinyu Wang,Weihua Zhao,Yaxuan Chen
Objective Insulin resistance (IR) plays a key role in gestational diabetes mellitus (GDM) pathogenesis. The antiaging protein klotho has been proven to be closely related to IR. The purpose of this study was to investigate the effect of klotho on IR in GDM trophoblast cells. Methods The GDM cell model of HTR-8/SVneo cells was induced by high glucose (HG). Plasmid transfection was used to mediate the
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Frequency of CYP2B6 Alleles in Major Iranian Ethnicities, Affecting Response to Efavirenz. Genet. Res. (IF 1.5) Pub Date : 2022-10-19 Parham Mardi,Bahareh Tavakoli-Far,Samira Sheibani Nia,Roshanak Jazayeri,Massoud Houshmand
Introduction Efavirenz is an antihuman immunodeficiency virus (HIV) drug metabolized by cytochrome P450 2B6 (CYP2B6) enzyme. Cytochrome P450 2B6 is an enzyme that in humans is encoded by the CYP2B6 gene. Polymorphisms of this gene play a crucial role in the metabolism of drugs such as Efavirenz. This study aims to evaluate the frequency of three clinically significant CYP2B6 polymorphisms (CYP2B6 ∗
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Bioinformatics Analysis Identifies TNFRSF1A as a Biomarker of Liver Injury in Sepsis TNFRSF1A is a Biomarker for Septic Liver Injury. Genet. Res. (IF 1.5) Pub Date : 2022-10-15 Shangxun Zhou,Wei Zhao,Junjie Li,Yang Huang,Jing Yang,Qianmei Wang,Yunyun Xu,Chujun Duan,Yutong Wang,Wen Yin
Sepsis is a severe disease with high mortality, and liver injury is an independent risk factor for sepsis morbidity and mortality. We analyzed co-differentially expressed genes (co-DEGs) to explore potential biomarkers and therapeutic targets for sepsis-related liver injury. Three gene expression datasets (GSE60088, GSE23767, and GSE71530) were downloaded from the Gene Expression Omnibus (GEO). DEGs
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Associations between Extracellular Matrix Protein 1 Gene Polymorphism and Progression of Liver Disease. Genet. Res. (IF 1.5) Pub Date : 2022-10-14 Xiuting He,Ting Liu,Rui Zhang,Xu Li
Background Our study aimed to investigate the relationship between extracellular matrix 1 (ECM1) gene polymorphism and progression of liver fibrosis in the Chinese population. Methods A total 656 patients with hepatitis B virus (HBV) infection and 298 healthy individuals of the Chinese Han population were recruited for a retrospective case-control study. Of the disease group, 104 cases had chronic
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Molecular Characterization of Wild and Cultivated Strawberry (Fragaria × ananassa) through DNA Barcode Markers. Genet. Res. (IF 1.5) Pub Date : 2022-10-11 Awais Qarni,Khushi Muhammad,Abdul Wahab,Amir Ali,Chandni Khizar,Izhar Ullah,Abeer Kazmi,Tahira Sultana,Asma Hameed,Muhammad Younas,Mehdi Rahimi
Background DNA barcoding is a useful technique for the identification, conservation, and diversity estimation at the species level in plants. The current research work was carried out to characterize selected Fragaria species from northern Pakistan using DNA barcode markers. Methodology. Initially, the efficacy of eight DNA barcode markers was analyzed based on the amplification and sequencing of the
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Overexpressed Histocompatibility Minor 13 was Associated with Liver Hepatocellular Carcinoma Progression and Prognosis. Genet. Res. (IF 1.5) Pub Date : 2022-10-03 Rui-Qing Zong,Hong-Yan Zhang,Xiao-Ying Li,Yi-Ran Li,Ying Chen
Among primary liver carcinoma cases, the proportion of liver hepatocellular carcinoma (LIHC) cases is 75%-85%. Current treatments for LIHC include chemotherapy, surgical excision, and liver transplantation, which are effective for early LIHC treatment. Nevertheless, the early symptoms of liver carcinoma are atypical, so a large proportion of LIHC patients are diagnosed at an advanced stage. Histocompatibility