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Expression of ERV3-1 in leukocytes of acute myelogenous leukemia patients Gene (IF 2.984) Pub Date : 2020-12-16 So Nakagawa; Masaharu Kawashima; Yuji Miyatake; Kai Kudo; Ryutaro Kotaki; Kiyoshi Ando; Ai Kotani
Acute myelogenous leukemia (AML) is one of the major hematological malignancies. In the human genome, several have been found to originate from retroviruses, and some of which are involved in the progression of various cancers. Hence, to investigate whether retroviral-like genes are associated with AML development, we conducted a transcriptome sequencing analysis of 12 retroviral-like genes of 150
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Detection and genetic characterization of porcine circovirus 4 (PCV4) in Guangxi, China Gene (IF 2.984) Pub Date : 2020-12-29 Wenchao Sun; Qian Du; Zhixiao Han; Jinshan Bi; Tian Lan; Wei Wang; Min Zheng
Porcine circovirus type 4 (PCV4), a novel circovirus, was identified in pigs with serious symptoms, including porcine dermatitis and nephropathy syndrome (PDNS)-like signs, in China in 2019. This study investigated the prevalence and genome diversity of PCV4 in pigs from Guangxi Province, China, between 2015 and 2019. Thirteen of 257 (5.1%) samples were positive for PCV4, 9 of these (69.2%) PCV4-positive
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Electron transfer flavoprotein and its role in mitochondrial energy metabolism in health and disease Gene (IF 2.984) Pub Date : 2021-01-13 Bárbara J. Henriques; Rikke Katrine Jentoft Olsen; Cláudio M. Gomes; Peter Bross
Electron transfer flavoprotein (ETF) is an enzyme with orthologs from bacteria to humans. Human ETF is nuclear encoded by two separate genes, ETFA and ETFB, respectively. After translation, the two subunits are imported to the mitochondrial matrix space and assemble into a heterodimer containing one FAD and one AMP as cofactors. ETF functions as a hub taking up electrons from at least 14 flavoenzymes
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Super-enhancers for RUNX3 are required for cell proliferation in EBV-infected B cell lines Gene (IF 2.984) Pub Date : 2021-01-12 Hiroki Hosoi; Akiko Nambu; Giselle Sek Suan Nah; Avinash Govind Bahirvani; Michelle Meng Huang Mok; Takaomi Sanda; Alan Prem Kumar; Daniel G. Tenen; Yoshiaki Ito; Takashi Sonoki; Motomi Osato
Epstein-Barr virus nuclear antigens 2 (EBNA2) mediated super-enhancers, defined by in silico data, localize near genes associated with B cell transcription factors including RUNX3. However, the biological function of super-enhancer for RUNX3 gene (seR3) remains unclear. Here, we show that two seR3s, tandemly-located at 59- and 70-kb upstream of RUNX3 transcription start site, named seR3 -59h and seR3
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Association study of rs7799039, rs1137101 and rs8192678 gene variants with disease susceptibility/severity and corresponding LEP, LEPR and PGC1A gene expression in multiple sclerosis Gene (IF 2.984) Pub Date : 2021-01-12 Ivana Kolić; Ljiljana Stojković; Aleksandra Stanković; Milan Stefanović; Evica Dinčić; Maja Zivković
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Genetic variants in the cholesterol biosynthesis pathway genes and risk of prostate cancer Gene (IF 2.984) Pub Date : 2021-01-12 Yifei Cheng; Yixuan Meng; Shuwei Li; Dongliang Cao; Shuai Ben; Chao Qin; Lixin Hua; Gong Cheng
Previous studies have found the relationship between cholesterol biosynthesis pathway genes and the risk or prognosis of prostate cancer (PCa), while there is no definite evidence that genetic variants in the cholesterol biosynthesis pathway gene is related to PCa risk. Consequently, we performed this study to explore the associations of single-nucleotide polymorphisms (SNPs) in the cholesterol biosynthesis
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Exome-wide scan identifies significant association of rs4788084 in IL27 promoter with increase in hepatic fat content among Indians Gene (IF 2.984) Pub Date : 2021-01-12 Ankita Chatterjee; Analabha Basu; Kausik Das; Abhijit Chowdhury; Priyadarshi Basu
Background Non-alcoholic fatty liver disease (NAFLD) is a global epidemic that often progresses to liver cirrhosis and hepatocellular carcinoma. In contrast to most world populations where NAFLD is mostly prevalent among obese, NAFLD among Indians and generally among South and South-East Asians is unique and highly prevalent among individuals who are lean. Genetics of NAFLD in Indian populations is
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Bone metastatic breast cancer cells display downregulation of PKC-ζ with enhanced glutamine metabolism Gene (IF 2.984) Pub Date : 2021-01-12 Manish Tandon; Ahmad H. Othman; Marcus Winogradzki; Jitesh Pratap
Background Breast cancer is the most commonly diagnosed cancer among women and its metastases results in poor survival rates in patients. The ability to alter metabolism is a key attribute cancer cells use to survive within different metastatic microenvironments and cause organ failure. We hypothesized that evaluation of metabolic alterations within tumor cells could provide a better understanding
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Screening and identification of haptoglobin showing its important role in pathophysiological process of gallbladder carcinoma Gene (IF 2.984) Pub Date : 2021-01-12 Li Guo; Mengting Liu; Yuyang Dou; Rui Duan; Lulu Shen; Lin Jia; Jun Wang; Changxian Li; Xiangcheng Li; Tingming Liang
Gallbladder cancer (GBC) with poor prognosis has been a major cause of cancer-related deaths worldwide. In this study, we aimed to screen and identify crucial genes in GBC through integrative analysis of multiple datasets and further experimental validation. A candidate crucial gene, up-regulated haptoglobin (HP), was firstly screened, and then further analysis and validation mainly focused on whether
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GPR10 gene deletion in mice increases basal neuronal activity, disturbs insulin sensitivity and alters lipid homeostasis Gene (IF 2.984) Pub Date : 2021-01-12 Veronika Pražienková; Jiří Funda; Zdenko Pirník; Alena Karnošová; Lucie Hrubá; Lucia Kořínková; Barbora Neprašová; Petra Janovská; Michal Benzce; Michaela Kadlecová; Jaroslav Blahoš; Jan Kopecký; Blanka Železná; Jaroslav Kuneš; Kristina Bardová; Lenka Maletínská
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Gender Bias in the Genetic Vulnerability Towards Type 2 Diabetes and Diabetic nephropathy: Role of Forkhead Box Protein3 Transcription Factor Gene Variants Gene (IF 2.984) Pub Date : 2021-01-12 Swetha Chikoti; Umme Najiya Mahwish; Sree Bhushan Raju; Gaddam. Sumanlatha; Parveen Jahan
Forkhead Box Protein3 Transcription Factor (FOXP3) gene is an essential role player in the function and differentiation of regulatory T cells. Polymorphisms/mutations in FOXP3 gene cause Treg cell dysfunction, promote autoimmunity and inflammation. Based on this presumption, we screened 600 subjects from south India (equal number of diabetic (T2DM), diabetic nephropathy (T2DN) and healthy controls)
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Effects of HSP27 gene expression on the resistance to Escherichia coli infection in piglets Gene (IF 2.984) Pub Date : 2021-01-12 Qiufang. Zong; Pengfei Jing; Shouyong Sun; Haifei Wang; Shenglong Wu; Wenbin Bao
Heat shock protein 27 (HSP27) plays an important role in protecting cells from various stress factors. This study aimed to investigate the function of HSP27 gene and its regulatory mechanism as infected by Escherichia coli (E. coli) at the tissue and cellular levels. Real-time PCR was used to detect the differential expression of HSP27 gene in F18 resistant and sensitive Sutai pigs and the differential
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Transcriptome analysis to identify key genes involved in terpenoid and rosmarinic acid biosynthesis in lemon balm (Melissa officinalis) Gene (IF 2.984) Pub Date : 2021-01-12 Mehdi Mansouri; Fatemeh Mohammadi
Melissa officinalis (lemon balm) is a well-known pharmaceutical plant in traditional medicine around the world because of the high-value secondary metabolites. Nowadays, advances in computational biology and bioinformatics have opened new avenues to plant-based natural product drug discovery. Despite the pharmacological importance, there is low information about the genes encoding the important biosynthetic
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Comparative analysis of chloroplast genomes of seven perennial Helianthus species Gene (IF 2.984) Pub Date : 2021-01-12 Kirill Azarin; Alexander Usatov; Maksim Makarenko; Vladimir Khachumov; Vera Gavrilova
Sequencing and a comparative analysis of the complete chloroplast genomes of seven perennial Helianthus species were carried out. The chloroplast genomes have a typical quadripartite structure, including large and small single regions and a pair of inverted repeats. Genome sizes were between 151,152 bp and 151,289 bp. The genome of H. grosseserratus was the smallest, while that of H. microcephalus
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Characterization of microbiologically influenced corrosion by comprehensive metagenomic analysis of an inland oil field Gene (IF 2.984) Pub Date : 2021-01-12 Badoor Nasser; Yoshimoto Saito; Mohammed Alarawi; Abdulmohsen A. Humam; Katsuhiko Mineta; Takashi Gojobori
Corrosion in pipelines and reservoir tanks in oil plants is a serious problem in the global energy industry because it causes substantial economic losses associated with frequent part replacement and can lead to potential damage to entire crude oil fields. Previous studies revealed that corrosion is mainly caused by microbial activities in a process currently termed microbiologically influenced corrosion
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Gene expression of type II collagen is regulated by direct interaction with Kruppel-like factor 4 and AT-rich interactive domain 5B Gene (IF 2.984) Pub Date : 2020-12-29 Xiang Zhang; Giang Thi Hien Nham; Kazuo Ito; Tamayuki Shinomura
We have previously found and characterized two pairs of enhancer elements, E1 and E2, in the type II collagen alpha 1 chain (COL2A1) gene. Subsequent studies have suggested that these enhancers function differently in the regulation of gene expression. For example, histone deacetylase 10 modifies only the E2 enhancer region to affect gene expression. Therefore, in this study, we aimed to clarify the
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The Escherichia coli QseB/QseC signaling is required for correct timing of replication initiation and cell motility Gene (IF 2.984) Pub Date : 2020-12-23 Dan Wu; Luvsandorj Baigalmaa; Yuan Yao; Guotao Li; Ming Su; Lifei Fan; Morigen
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A reliable, quick and universally applicable method for monitoring aptamer SELEX progress Gene (IF 2.984) Pub Date : 2021-01-11 Shahnawaz A. Baba; Shubham Jain; Naveen K. Navani
Nucleic acid aptamers for biosensing are developed from a complex ssDNA library through Systematic Evolution of Ligands by Exponential Enrichment (SELEX) process. Monitoring of SELEX process is crucial for generating high-affinity aptamers. Extant methods for monitoring aptamer selection are either arduous or give false-positive signals, which adversely impact the outcome of selection. We describe
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Immunogene expression analysis in betanodavirus infected-Senegalese sole using an OpenArray® platform Gene (IF 2.984) Pub Date : 2021-01-11 Juan J. Gémez-Mata; Alejandro M. Labella; Isabel Bandín; Juan J. Borrego; Esther García-Rosado
The transcriptomic response of Senegalese sole (Solea senegalensis) triggered by two betanodaviruses with different virulence to that fish species has been assessed using an OpenArray® platform based on TaqMan™ quantitative PCR. The transcription of 112 genes per sample has been evaluated at two sampling times in two organs (head kidney and eye/brain-pooled samples). Those genes were involved in several
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Vitamin E can promote spermatogenesis by regulating the expression of proteins associated with the plasma membranes and protamine biosynthesis Gene (IF 2.984) Pub Date : 2021-01-05 Yuefeng Gao; Luyang Jian; Wei Lu; Ying Xue; Zoltan Machaty; Hailing Luo
Vitamin E is generally believed to promote the production of ovine sperm mainly through its antioxidant effect. Our previous studies have shown that some non-antioxidant genes may also be key in mediating this process. The objective of this study was to identify key candidate proteins that were differentially expressed in response to a treatment with Vitamin E. Prepubertal ovine testicular cells were
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Progress and Prospect of Single-molecular ClpX ATPase Researching system-a mini-review Gene (IF 2.984) Pub Date : 2021-01-09 Zhen-Hui Kang; Yi-Ting Liu; Yang Gou; Qi-Rui Deng; Zi-yu Hu; Guan-Rong Li
ClpXP in Escherichia coli is a proteasome degrading protein substrates. It consists of one hexamer of ATPase (ClpX) and two heptamers of peptidase (ClpP). The ClpX binds ATP and translocates the substrate protein into the ClpP chamber by binding and hydrolysis of ATP. At single molecular level, ClpX harnesses cycles of power stroke (dwell and burst) to unfold the substrates, then releases the ADP and
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Molecular cloning, characterization, and expression analysis of a sex-biased transcriptional factor sox9 gene of mud crab Scylla paramamosain Gene (IF 2.984) Pub Date : 2021-01-09 Haifu Wan; Jiaqian Liao; Ziping Zhang; Xianyuan Zeng; Keying Liang; Yilei Wang
Sox9 gene, a crucial member of the Sox gene family, is present in various organisms and involved in many physiological processes, especially in sex determination and gonad development. In this study, we reported a sox9 gene (designated as Spsox9) from Scylla paramamosain through analyzing published gonad transcriptome data. Meanwhile, the accuracy was validated by PCR technology, and the 3’ sequences
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Relative expression of putative genes involved in galanthamine and other Amaryllidaceae alkaloids biosynthesis in Narcissus field and in vitro tissues Gene (IF 2.984) Pub Date : 2021-01-09 Ferdausi Aleya; Chang Xianmin; Hall Anthony; Jones Meriel
The Narcissus pseudonarcissus cv. Carlton contains Amaryllidaceae alkaloids namely galanthamine, lycorine, homolycorine, narciclasine, which are noted for their pharmaceutical properties such as for the treatment of early to mid-stage Alzheimer's diseases, cancer, tumor etc. Alkaloid biosynthesis using plant in vitro systems has been considered as a tool for drug discovery and the pathways are starting
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Copy number variation of the HPGDS gene in the Ashidan yak and its associations with growth traits Gene (IF 2.984) Pub Date : 2020-12-26 Chun Huang; Fei Ge; Wenwen Ren; Yongfeng Zhang; Xiaoyun Wu; Qiang Zhang; Xiaoming Ma; Pengjia Bao; Xian Guo; Min Chu; Ping Yan; Chunnian Liang
Copy number variation (CNV) is a structural variation at the submicroscopic level of the genome, which can affect gene-related phenotypes by changing genes dosage and transcript structure. Hematopoietic prostaglandin D synthase (HPGDS) is a member whose functions are closely related to weight gain and inflammatory diseases of the glutathione S-transferase (GSTs) family. In this study, the growth characteristics
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NBIGV-DB: A dedicated database of non-B cell derived immunoglobulin variable region Gene (IF 2.984) Pub Date : 2020-12-23 Chi Zhang; Lin Xiao; Yan Huang; Li Zhang; Dongyang Jiang; Wenwei Shao; Jie Zheng; Fanlei Hu; Ming Chu; Jing Huang; Xiaoting Gong; Yuan Zhou; Xiaoyan Qiu
Immunoglobulins (Ig) are important immune molecules that possess highly diverse variable region sequences enabling antigen recognition. According to classical immune theory, B lymphocytes have been considered the only source of Ig production (B-Igs). However, accumulating evidence have suggested that Igs are also produced by many non-B cells (non-B Igs), including epithelial cells, neurons, germ cells
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Adaptive evolution of β-globin gene in subterranean in South America octodontid rodents Gene (IF 2.984) Pub Date : 2020-12-20 Mariana Pejo; Ivanna H. Tomasco
The convergent evolution of subterranean rodents is an excellent model to study how natural selection operates and the genetic bases of these adaptations, but the study on the different taxa has been very uneven and still insufficient. In the octodontoid caviomorph rodent superfamily there are two independent lineages where they have recently evolved into totally underground lifestyles: the genera
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Genetic evidence of shared ancestry among diverse ethno-linguistic human populations of Himachal Pradesh Gene (IF 2.984) Pub Date : 2020-12-23 Abhishek Singh; Vivek Sahajpal; Satish Kumar; Malay Shukla; Kailash Chandra; Lalit Kumar Sharma; Mukesh Thakur
In Indian montane system, human populations often exhibit an unparallel social organization where inter-caste marriages are still not common. This attribute affects the demography and population genetic structure of the resident populations. Further, human populations residing in the mountains in India are poorly studied for their genetic make-up and allele distribution patterns. In the present study
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Identification of functional methylated CpG loci in PD-L1 promoter as the novel epigenetic biomarkers for primary gastric cancer Gene (IF 2.984) Pub Date : 2020-12-23 Mohammad Amini; Maryam Hejazi; Khodayar Ghorban; Ahad Mokhtarzadeh; Behzad Baradaran
Gastric cancer (GC) is considered one of the most lethal malignancies worldwide due to poor prognosis. Aberrant methylation has been demonstrated to be involved in PD-L1 dysregulated expression in human cancers and possesses a great value as a diagnostic biomarker. Given that, in this study, we investigated the methylation status of PD-L1 as a promising biomarker in primary gastric tumors and identified
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Upregulation of potential regulatory signaling molecules correlate with androgen receptor splice variants AR-V7 and AR-V567es in prostate cancer metastasis Gene (IF 2.984) Pub Date : 2021-01-05 Ilker Kiliccioglu; Cenk Y. Bilen; Sinan Sozen; Ece Konac
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Transcriptome sequencing and differential gene expression analysis reveal the mechanisms involved in seed germination and protocorm development of Calanthe tsoongiana Gene (IF 2.984) Pub Date : 2021-01-05 Yating Jiang; Min Tian; Caixia Wang; Ying Zhang
Calanthe tsoongiana is a rare orchid species native to China. Asymbiotic seed germination is of great importance in the ex situ conservation of this species. Based on morphological characteristics and anatomical structures, the C. tsoongiana developmental process from seeds to seedlings was divided into four stages (SA, PB, PC and PD), and subsequently, changes in endogenous hormone contents and gene
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Potential roles of extracellular vesicles in osteonecrosis of femoral head: A systematic review Gene (IF 2.984) Pub Date : 2020-12-30 Xinjie Wu; Yanlei Wang; Wei Sun; Mingsheng Tan
Extracellular vesicles (EVs), have been utilised for the diagnosis and treatment of osteonecrosis of femoral head (ONFH). Hence, we aimed to review the available evidence on the potential roles of EVs in ONFH systematically. The PubMed, EMBASE, and Science Citation Index databases were searched exhaustively from inception to June 2020. All in vitro and in vivo studies on the use of EVs in ONFH diagnosis
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Silencing FAM135B enhances radiosensitivity of esophageal carcinoma cell Gene (IF 2.984) Pub Date : 2020-12-17 Liangwen Bi; Haijing Wang; Ye Tian
FAM135B (family with sequence similarity 135, member B) is related to the progression of esophageal squamous cell carcinoma (ESCC). However, the role played by the gene in radiosensitivity remains unknown. Herein, we examined the relationship between FAM135B and radiosensitivity. According to the results, FAM135B is highly expressed in ESCC cells, and ESCC cells with high levels of FAM135B are resistant
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The kinase CIPK11 functions as a positive regulator in cadmium stress response in Arabidopsis Gene (IF 2.984) Pub Date : 2020-12-18 Shaobo Gu; Xin Wang; Jiuyuan Bai; Tao Wei; Manli Sun; Lin Zhu; Maolin Wang; Yun Zhao; Wei Wei
Cadmium (Cd) pollution in agricultural soil has always been a knotty problem, which made it necessary to find the mechanism related to Cd transport in plant. In this study, we found a novel character of the CIPK11 modulating the transport of Cd in Arabidopsis thaliana. Over-expression of CIPK11 (CIPK11OE#1-7, CIPK11OE#8-5) resulted in the increased tolerance to Cd stress, which embodied in higher fresh
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New insights into TNFα/PTP1B and PPARγ pathway through RNF213- a link between inflammation, obesity, insulin resistance, and Moyamoya disease Gene (IF 2.984) Pub Date : 2020-12-15 Priyanka Sarkar; Kavitha Thirumurugan
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Characterization of the Drosophila suzukii β2-tubulin gene and the utilization of its promoter to monitor sex separation and insemination Gene (IF 2.984) Pub Date : 2020-12-18 Ying Yan; Jonas Schwirz; Marc F. Schetelig
The Drosophila melanogaster β2-tubulin gene (Dm-β2t) controls the function of microtubules in the testis and sperm, and has been evaluated for use in biocontrol strategies based on the sterile insect technique, including sexing and the induction of male sterility. The spotted-wing Drosophila (Drosophila suzukii) is native to eastern Asia but has spread globally as an invasive pest of fruit crops, so
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Whole-genome resequencing reveals diversity and selective signals in Longlin goat Gene (IF 2.984) Pub Date : 2020-12-17 Qiuming Chen; Yingfei Huang; Zihao Wang; Shaohua Teng; Quratulain Hanif; Chuzhao Lei; Junli Sun
The Longlin goat is one of the most valuable livestock species in Guangxi Autonomous Region of China, but its genomic diversity and selective signals are not clearly elucidated. Here we compared 20 genomes of Longlin goat to 66 genomes of other seven goat breeds worldwide to analyze patterns of Longlin goat genetic variation. We found the lowest linkage disequilibrium at the large distances between
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Identification of novel Taz isoforms and functional comparison in pluripotency maintenance of mouse embryonic stem cells Gene (IF 2.984) Pub Date : 2020-12-29 Yixiao Xu; Lianlian Liu; Xueyue Wang; Yuyan Huang; Yuda Cheng; Junlei Zhang; Jiali Wang; Yanping Tian; Jiaxiang Xiong; Yi Yang; Bangqi Ren; Wei Wu; Ping He; Yue Zhang; Binyu Zhao; Jiaqi Wang; Meng Yu; Jiangjun Wang; Yan Ruan
Alternative splicing (AS) is a key process to expand the diversity of mRNA and protein from the genome and it is crucial for fate determination of embryonic stem cells (ESCs) by encoding isoforms with different functions to regulate the balance between pluripotency maintenance and differentiation. Since the role of the Hippo pathway in ESCs is controversial, there may be novel isoforms of Taz, a key
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Deciphering the multi-scale mechanisms of Tephrosia purpurea against polycystic ovarian syndrome (PCOS) and its major psychiatric comorbidities: studies from network pharmacological perspective Gene (IF 2.984) Pub Date : 2020-12-29 Neha Choudhary; Shilpa Choudhary; Arun Kumar; Vikram Singh
Tephrosia purpurea (T. purpurea), a plant belonging to Fabaceae (pea) family, is a well-known Ayurvedic herb and commonly known as Sarapunkha in traditional Indian medicinal system. Described as “Sarwa wranvishapaka”, i.e. having a capability to heal all types of wounds, it is particularly recognized for its usage in splenomegaly. Towards exploring the comprehensive effects of T. purpurea against polycystic
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ANXA1 affects murine hair follicle growth through EGF signaling pathway Gene (IF 2.984) Pub Date : 2020-12-14 Hao Zhang; Xiaoqiao Fu; Yue Ao; Miaomiao Nan; Ziyu Qiu; Xiuzhi Jia; Ying Xiao; Dongjun Liu; Xudong Guo
Annexin A1 (ANXA1), a calcium-dependent phospholipid binding protein expressed in animals, plants and microorganisms, participates in various cellular physiological activities. Previous proteomics analysis indicates that the level of ANXA1 in mice dorsal skin changes during hair growth cycle, we speculate that ANXA1 may play an important role in hair follicle (HF) development. Thus, Anxa1 knock-out
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Adiponectin promotes myogenic differentiation via a Mef2C-AdipoR1 positive feedback loop Gene (IF 2.984) Pub Date : 2020-12-28 An Yu; Yu Zheng; Yuqing Gong; Ruofan Yu; Zaiqing Yang; Xiaodong Chen
Adiponectin is an important hormone that regulates systemic metabolism, and it has been reported that globular adiponectin promotes myogenic differentiation. However, the mechanisms by which adiponectin promotes myogenic differentiation is not fully understood. In the present study, we show that adiponectin and its receptor 1 are significantly up-regulated during myogenic differentiation and that adiponectin
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Hypoxia-reoxygenation stress modulates the hepatopancreas transcriptome of Chinese mitten crab Eriocheir sinensis Gene (IF 2.984) Pub Date : 2020-12-16 Yu Xu; Zhen Miao; Xuguang Li; Hai Lin; Yu Cheng; Jianlin Pan; Zhiqiang Xu
Hypoxia is a critical, but frequently overlooked problem, which commonly exists in Chinese mitten crab rearing. However, little information is available on the molecular mechanisms of the detrimental effects of hypoxia in this species. In the present study, crabs were subjected to acute hypoxia stress (DO 1.0 mg/L), followed by reoxygenation (DO 6.8 mg/L). Hepatopancreas from five groups of crabs (three
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Circular RNAs: Promising biomarkers for cancer diagnosis and prognosis Gene (IF 2.984) Pub Date : 2020-12-18 Guohao Wei; Jing Zhu; Hai-Bo Hu; Jia-Qiang Liu
Circular RNAs (circRNAs), a group of non-coding RNA characterized by the presence of covalent bonds linking 3′ and 5′ ends, act as miRNA sponges to participate in the tumorigenesis. Being stable, conserved and cell- or tissue-specific, circRNAs have shown their potentials as molecular markers for cancer. Convenient and noninvasive approaches may be developed based on the roles of circRNAs to diagnose
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Recognition of plausible therapeutic agents to combat COVID-19: An omics data based combined approach Gene (IF 2.984) Pub Date : 2020-12-17 Mohammad Uzzal Hossain; Arittra Bhattacharjee; Md. Tabassum Hossain Emon; Zeshan Mahmud Chowdhury; Md. Golam Mosaib; Muntahi Mourin; Keshob Chandra Das; Chaman Ara Keya; Md. Salimullah
Coronavirus disease-2019 (COVID-19), caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), has become an immense threat to global public health. In this study, we performed complete genome sequencing of a SARS-CoV-2 isolate. More than 67,000 genome sequences were further inspected from Global Initiative on Sharing All Influenza Data (GISAID). Using several in silico techniques, we
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Inhibitory role of oleanolic acid and esculetin in HeLa cells involve multiple signaling pathways Gene (IF 2.984) Pub Date : 2020-12-18 Prajitha Mohandas Edathara; Shivakanth Chintalapally; Venkata Krishna Kanth Makani; Chitrakshi Pant; Suresh Yerramsetty; Manohar D. Rao; Manika Pal Bhadra
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Comparative genomics with a multidrug-resistant Klebsiella pneumoniae isolate reveals the panorama of unexplored diversity in Northeast Brazil Gene (IF 2.984) Pub Date : 2020-12-26 Rodrigo Profeta; Núbia Seyffert; Sandeep Tiwari; Marcus V.C. Viana; Arun Kumar Jaiswal; Ana Carolina Caetano; Daniel Henrique Bücker; Luciana Tavares de Oliveira; Roselane Santos; Alfonso Gala-Garcia; Rodrigo B. Kato; Francine F. Padilha; Isabel B. Lima-Verde; Preetam Ghosh; Debmalya Barh; Aristóteles Góes-Neto; Henrique C.P. Figueiredo; Siomar C. Soares; Thiago L.P. Castro
The emergence of community acquired infections increases the public health concern on K. pneumoniae and closely related bacteria among which antimicrobial resistance spreads. We report a multidrug-resistant K. pneumoniae isolate, B31, of a patient infected in the community and admitted to an intensive care unit in Northeast Brazil. Antimicrobial susceptibility and genome information were thoroughly
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Molecular profiling and functional delineation of peroxiredoxin 3 (HaPrx3) from the big-belly seahorses (Hippocampus abdominalis) and understanding their immunological responses Gene (IF 2.984) Pub Date : 2020-12-15 Anushka Vidurangi Samaraweera; M.D. Neranjan Tharuka; Thanthrige Thiunuwan Priyathilaka; Hyerim Yang; Sukkyoung Lee; Jehee Lee
Peroxiredoxins (Prxs) are ubiquitously expressed antioxidant proteins that can protect aerobic organisms from oxidative stress. Here, we characterized the HaPrx3 homolog at the molecular level from big-belly seahorse (Hippocampus abdominalis) and analyzed its functional activities. The coding sequence of HaPrx3 consists of 726 bp, which encodes 241 amino acids. The predicted molecular weight and theoretical
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Genetic factors in the pathogenesis of ameloblastoma, dentigerous cyst and odontogenic keratocyst Gene (IF 2.984) Pub Date : 2020-12-17 Soudeh Ghafouri-Fard; Saede Atarbashi-Moghadam; Mohammad Taheri
Ameloblastoma (AB), dentigerous cyst (DC) and Odontogenic keratocyst (OKC) are odontogenic lesions with propensity to malignant transformation or local invasion. The molecular mechanisms of development of these lesions are not fully understood. However, some researches have reported dysregulation of tumor suppressor genes or oncogenes in these lesions. Down-regulation of P53 gene has been reported
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Single-cell profiles of human bone marrow-derived mesenchymal stromal cells after IFN-γ and TNF-α licensing Gene (IF 2.984) Pub Date : 2020-12-15 Shuanglong Lu; Xiaohong Qiao
Background Pre-licensing mesenchymal stromal cells (MSCs) with IFN-γ and TNF-α can empower their immune fate and induce a more effective immune regulation. However, the cellular heterogeneity of MSCs limits our understanding of this inflammatory licensing. Methods The publicly available Gene Expression Omnibus single-cell RNA sequencing (scRNA-seq) data of human bone marrow-derived MSCs with or without
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Association of xenobiotic receptor polymorphisms with carbamazepine response in epilepsy patients Gene (IF 2.984) Pub Date : 2020-12-15 Fan-Cheng Kong; Chun-Lai Ma; Li-Qin Lang; Ming-Kang Zhong
Purpose Drug-resistant epilepsy is a problem worldwide. Xenobiotic receptors may play a significant role in the establishment of resistance to antiepileptic agents. Previous studies have confirmed that the metabolism and efficacy of carbamazepine (CBZ) can be influenced by xenobiotic receptors, especially pregnane X receptor (PXR), constitutive androstane receptor (CAR), and aryl hydrocarbon receptor
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Some pathogenic SETX variants are partially conserved during evolution Gene (IF 2.984) Pub Date : 2020-12-15 Huma Tariq; Iqra Tariq; Thomas Bourinaris; Henry Houlden; Sadaf Naz
Variants in SETX have been implicated in recessively and dominantly inherited disorders, ataxia with oculomotor apraxia type 2 (AOA2 OMIM# 606002) and amyotrophic lateral sclerosis (ALS4, OMIM# 602433) respectively, in humans. We report two novel bi-allelic pathogenic variants in SETX in patients suffering from ataxia with oculomotor apraxia type 2, extending the allelic spectrum of the gene variants
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Down regulation of VDR gene expression in metabolic syndrome and atherosclerosis’ patients: Cause or consequence? Gene (IF 2.984) Pub Date : 2020-12-15 Maria Eduarda de Albuquerque Borborema; Dinaldo C. Oliveira; Jaqueline de Azevêdo Silva
Background Metabolic syndrome (MS) features a set of clinical manifestations with an increased risk of atherosclerosis development. Vitamin D3 (VD3) pathway influences upon clinical features in MS as well in the formation of atherosclerotic plaque. VD3 acts through the vitamin D receptor (VDR), regulating the transcription of several genes involved in the immune response, growth and homeostasis. Aim
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Phylogeography of sika deer (Cervus nippon) inferred from mitochondrial cytochrome-b gene and microsatellite DNA Gene (IF 2.984) Pub Date : 2020-12-23 Huamiao Liu; Yan Ju; Hidetoshi Tamate; Tianjiao Wang; Xiumei Xing
The genetic diversity and phylogenetic relationships of sika deer of different subspecies are uncertain. In order to explore the phylogenetic relationship of different sika deer subspecies, this study used a wider sample collection to analyze mitochondrial sequences and nuclear microsatellites of sika deer. The full lengths of cytochrome-b gene of 134 sika deer were sequenced, and 16 haplotypes were
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Puerarin alleviates coronary heart disease via suppressing inflammation in a rat model Gene (IF 2.984) Pub Date : 2020-12-15 Liangping Zhao; Li Wang; Daimin Zhang; Yuqi Chen; Fulu Jin
Background Puerarin shows inhibitory effects on inflammation in chronic heart failure (CHF), but its efficacy in coronary heart disease (CHD) remained vague. Methods Rat CHD model was constructed, and serum parameters were determined using a blood liquid biochemical analyzer. Also, contents of creatine kinase (CK), creatine kinase MB isoenzyme (CK-MB), lactate dehydrogenase (LDH) and cardiac troponin
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High expression of MYEOV reflects poor prognosis in non-small cell lung cancer Gene (IF 2.984) Pub Date : 2020-12-02 Rui Zhang; Aiqing Ma
Background The myeloma overexpressed gene (MYEOV) plays a critical role in tumorigenesis in a variety of cancers. However, little is known of the prognosis and immune infiltration associated with MYEOV in non-small cell lung cancer (NSCLC). Methods We used several databases (Oncomine, TCGA, and GEO) to analysis the expression, prognosis, and immune infiltration, associated with MYEOV in NSCLC. We also
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Comparison of gene mutation spectra in younger and older Chinese acute myeloid leukemia patients and its prognostic value Gene (IF 2.984) Pub Date : 2020-12-14 Wei-jie Zhong; Xiu-dan Liu; Li-ye Zhong; Kang-bao Li; Qi-xin Sun; Xin Xu; Ting Wei; Qing-shan Li; Zhi-gang Zhu
Differences in the gene mutation spectra of younger and older Chinese adult AML patients and the prognostic significance of these differentially presented gene mutations are rarely reported. One hundred and thirteen newly diagnosed Chinese adults with AML, divided into groups of younger and older patients, were enrolled in this study. Bone marrow samples from the patients were analyzed using targeted
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Genome-wide association study of flowering time reveals complex genetic heterogeneity and epistatic interactions in rice Gene (IF 2.984) Pub Date : 2020-12-15 Chang Liu; Yuan Tu; Shiyu Liao; Xiangkui Fu; Xingming Lian; Yuqing He; Weibo Xie; Gongwei Wang
Since domestication, rice has cultivated in a wide range of latitudes with different day lengths. Selection of diverse natural variations in heading date and photoperiod sensitivity is critical for adaptation of rice to different geographical environments. To unravel the genetic architecture underlying natural variation of rice flowering time, we conducted a genome wide association study (GWAS) using
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RNA-sequencing reveals transcriptional signature of pathological remodeling in the diaphragm of rats after myocardial infarction Gene (IF 2.984) Pub Date : 2020-12-15 Svetlana Yegorova; Oleg Yegorov; Leonardo F. Ferreira
The diaphragm is the main inspiratory muscle, and the chronic phase post-myocardial infarction (MI) is characterized by diaphragm morphological, contractile, and metabolic abnormalities. However, the mechanisms of diaphragm weakness are not fully understood. In the current study, we aimed to identify the transcriptome changes associated with diaphragm abnormalities in the chronic stage MI. We ligated
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Interactive associations of the INAFM2 rs67839313 variant and egg consumption with type 2 diabetes mellitus and fasting blood glucose in a Chinese population: A family-based study Gene (IF 2.984) Pub Date : 2020-12-14 Xiaowen Wang; Zijing Wang; Junhui Wu; Mengying Wang; Jiating Wang; Tao Wu; Dafang Chen; Xun Tang; Xueying Qin; Yiqun Wu; Yonghua Hu
Background INAFM2 rs67839313 was associated with type 2 diabetes (T2DM) in Japanese populations but not in other populations. We aimed to validate the association of rs67839313 with T2DM and explore interactive associations of INAFM2 rs67839313 and egg consumption with T2DM and fasting blood glucose (FBG) in a Chinese population. Methods In total, 7175 participants (4202 T2DM cases) from 3980 families
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Krüppel-like factor 12 suppresses bladder cancer growth through transcriptionally inhibition of enolase 2 Gene (IF 2.984) Pub Date : 2020-12-03 Cai Tang; Miao Wang; Yi Dai; Xin Wei
Krüppel-like factors (KLFs) are transcription factors and play important roles in bladder cancer (BC). Clarifying the function of KLFs will provide new strategies for clinical treatment of BC. In this study, we found that Krüppel-like factor 12 (KLF12) was decreased in BC tissues and cells. Knockdown of KLF12 by siRNA dramatically elevated the proliferation and colony formation of BC cells. By contrast
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Serine/Threonine Phosphatases in Osteoclastogenesis and Bone Resorption Gene (IF 2.984) Pub Date : 2020-12-16 Ismael Y. Karkache; Jeyaram R. Damodaran; David H.H. Molstad; Elizabeth W. Bradley
Maintenance of optimal bone mass is controlled through the concerted functions of several cell types, including bone resorbing osteoclasts. Osteoclasts function to remove calcified tissue during developmental bone modeling, and degrade bone at sites of damage during bone remodeling. Changes to bone homeostasis can arise with alterations in osteoclastogenesis and/or catabolic activity that are not offset