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Brazil nut consumption reduces DNA damage in overweight type 2 diabetes mellitus patients Mutat. Res. (IF 1.9) Pub Date : 2024-02-28 Tamires Pavei Macan, Marina Lummertz Magenis, Adriani Paganini Damiani, Isadora de Oliveira Monteiro, Gustavo De Bem Silveira, Rubya Pereira Zaccaron, Paulo Cesar Lock Silveira, João Paulo Fernandes Teixeira, Goran Gajski, Vanessa Moraes de Andrade
Type 2 diabetes mellitus (T2D) is a metabolic disease, which occurs largely due to unhealthy lifestyle. As oxidative stress is believed to promote T2D, by inducing damage to lipids, proteins, and DNA, appropriate dietary interventions seem critical to prevent, manage, and even reverse this condition. Brazil nuts (Bertholletia excelsa, H.B.K.) are nature’s richest source of selenium, a mineral that
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Multi-endpoint in vitro toxicological assessment of snus and tobacco-free nicotine pouch extracts Mutat. Res. (IF 1.9) Pub Date : 2024-02-20 Fan Yu, Emma Bishop, Fabio Miazzi, Rhian Evans, David Smart, Damien Breheny, David Thorne
‘Modern’ oral tobacco-free nicotine pouches (NPs) are a nicotine containing product similar in appearance and concept to Swedish snus. A three-step approach was taken to analyse the biological effects of NPs and snus extracts ToxTracker was used to screen for biomarkers for oxidative stress, cell stress, protein damage and DNA damage. Cytotoxicity, mutagenicity, and genotoxicity were assessed in the
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Constitutional chromosomal anomalies in children, fetal alcohol syndrome, and maternal toxicant exposures: A longitudinal cohort study Mutat. Res. (IF 1.9) Pub Date : 2024-02-09 David A. Geier, Mark R. Geier
DNA alterations in gametes, which may occur either spontaneously or as a result of exposure to genotoxicants, can lead to constitutional chromosomal anomalies in the offspring. Alcohol is an established genotoxicant. The goal of this hypothesis-testing longitudinal cohort study was to evaluate the effect of significant/sustained maternal alcohol exposure on clinically diagnosed constitutional chromosomal
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Hydrogen inhalation: in vivo rat genotoxicity tests Mutat. Res. (IF 1.9) Pub Date : 2024-02-01 Cordélia Salomez-Ihl, Stéphane Tanguy, Jean-Pierre Alcaraz, Chloé Davin, Victor Pascal-Moussellard, Mariem Jabeur, Pierrick Bedouch, Ludovic Le Hegarat, Valérie Fessard, Anne-Louise Blier, Sylvie Huet, Philippe Cinquin, François Boucher
Preclinical and clinical studies have shown that molecular hydrogen (H) has anti-oxidant, anti-inflammatory, and anti-apoptotic properties. Safety data are available in the literature and acute toxicity has been tested in isolated cells and laboratory animals. We have evaluates the genotoxicity of H in rats after 72 h exposure, following the International Council for Harmonization guidelines ICH S2
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Possible contribution of 8-hydroxydeoxyguanosine to gene mutations in the kidney DNA of gpt delta rats following potassium bromate treatment Mutat. Res. (IF 1.9) Pub Date : 2024-01-22 Ken Kuroda, Yuji Ishii, Shinji Takasu, Aki Kijima, Kohei Matsushita, Ken-ichi Masumura, Takehiko Nohmi, Takashi Umemura
8-Hydroxydeoxyguanosine (8-OHdG) is well known not only as an effective biomarker of oxidative stress but also as a mutagenic DNA modification. Incorporation of dAMP at the opposite site of 8-OHdG induces G>T or A>C transversions. However, in vivo analyses of gene mutations caused by potassium bromate (KBrO3), which can induce 8-OHdG at carcinogenic target sites, showed that G>T was prominent in the
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Persons chronically exposed to low doses of ionizing radiation: A cytogenetic dosimetry study Mutat. Res. (IF 1.9) Pub Date : 2024-01-18 Oksana Cherednichenko, Anastassiya Pilyugina, Serikbai Nuraliev, Dinara Azizbekova
The dosimetry and control of exposure for individuals chronically exposed to ionizing radiation are important and complex issues. Assessment may be optimized by evaluating individual adaptation and radiosensitivity, but it is not possible for a single model to account for all relevant parameters. Our goal was to develop approaches for the calculation of doses for persons chronically exposed to ionizing
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Genotoxicity of ultraviolet light and sunlight in the bacterium Caulobacter crescentus: Wavelength-dependence Mutat. Res. (IF 1.9) Pub Date : 2024-01-17 Fabiana Fuentes-León, Nathalia Quintero-Ruiz, Frank S. Fernández-Silva, Veridiana Munford, Marioly Vernhes Tamayo, Carlos Frederico Martins Menck, Rodrigo S. Galhardo, Angel Sánchez-Lamar
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Occupational exposure to radiation among health workers: Genome integrity and predictors of exposure Mutat. Res. (IF 1.9) Pub Date : 2024-01-11 Hayal Çobanoğlu, Akın Çayır
The current study aimed to investigate genomic instabilities in healthcare workers who may experience varying levels of radiation exposure through various radiological procedures. It also sought to determine if factors related to the work environment and dosimeter reading could effectively explain the observed genomic instabilities. Utilizing the cytokinesis-block micronucleus assay (CBMN) on peripheral
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The probable reasons of arsenic susceptibility in a chronically exposed population of West Bengal Mutat. Res. (IF 1.9) Pub Date : 2024-01-12 Ashok Kumar Giri, Nilanjana Banerjee
Arsenic is potent human carcinogen which affects millions of people across the globe. Arsenic induced pre-cancerous and cancerous skin lesions are hall marks of chronic arsenic toxicity. Even then, only 15%–20% of the population manifest arsenic-induced skin lesions but the rest do not, the reason for which in not very clear. Not only that, conjunctival irritations of the eyes, peripheral neuropathy
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The buccal micronucleus cytome assay: New horizons for its implementation in human studies Mutat. Res. (IF 1.9) Pub Date : 2024-01-06 Michael Fenech, Siegfried Knasmueller, Armen Nersesyan, Claudia Bolognesi, Georg Wultsch, Christian Schunck, Emanuela Volpi, Stefano Bonassi
In this report we provide a summary of the presentations and discussion of the latest knowledge regarding the buccal micronucleus (MN) cytome assay. This information was presented at the HUMN workshop held in Malaga, Spain, in connection with the 2023 European, Environmental Mutagenesis and Genomics conference. The presentations covered the most salient topics relevant to the buccal MN cytome assay
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The assessment and communication of genotoxicity test results: moving beyond binary Mutat. Res. (IF 1.9) Pub Date : 2023-12-24 Takashi Omori, Makoto Hayashi
Potential genotoxicity is one of the essential considerations in the safety assessment of chemicals to which humans may be exposed. Several endpoints are used to evaluate genotoxicity, but, in each case, a binary assessment (negative/positive) is demanded by regulators. The use of binary assessment has rarely been questioned, although we have pointed out some questions and difficulties with regard
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Genotoxicity and the stability of N-nitrosomorpholine activity following UVA irradiation Mutat. Res. (IF 1.9) Pub Date : 2023-12-02 Haruna Mochizuki, Yukari Nagazawa, Sakae Arimoto-Kobayashi
This study investigated N-nitrosomorpholine (NMOR) genotoxicity following UVA irradiation without metabolic activation. Following UVA irradiation, the photo treated NMOR (irradiated NMOR) was directly mutagenic, without UVA or metabolic activation, in the Ames test. The activity was relatively stable, and approximately 79% of the activity remained after 10 days of storage at 37 °C, 4 °C, or −20 °C
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Mutagenicity assessment of two potential impurities in preparations of 5-amino-2,4,6 triiodoisophthalic acid, a key intermediate in the synthesis of the iodinated contrast agent iopamidol Mutat. Res. (IF 1.9) Pub Date : 2023-11-28 Silvia Rossi, Simona Bussi, Roberta Bonafè, Carola Incardona, Emanuela Vurro, Massimo Visigalli, Federica Buonsanti, Roberta Fretta
5-Aminoisophthalic acid and 5-nitroisophthalic acid (5-NIPA) are potential impurities in preparations of 5-amino-2,4,6-triiodoisophthalic acid, which is a key intermediate in the synthesis of the iodinated contrast agent iopamidol. We have studied their mutagenicity in silico (quantitative structure-activity relationships, QSAR) and by the bacterial reverse mutation assay (Ames test). First, the compounds
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Oxidative DNA damage: Induction by fructose, in vitro, and its enhancement by hydrogen peroxide Mutat. Res. (IF 1.9) Pub Date : 2023-11-26 Kaoru Midorikawa, Kokoro Kobayashi, Shinya Kato, Shosuke Kawanishi, Hatasu Kobayashi, Shinji Oikawa, Mariko Murata
Sucrose and high-fructose corn syrup comprise nearly equal amounts of glucose and fructose. With the use of high-fructose corn syrup in the food industry, consumption of fructose, which may be a tumor promoter, has increased dramatically. We examined fructose-induced oxidative DNA damage in the presence of Cu(II), with or without the addition of H2O2. With isolated DNA, fructose induced Cu(II)-mediated
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Assessment of the performance of the Ames MPF™ assay: A multicenter collaborative study with six coded chemicals Mutat. Res. (IF 1.9) Pub Date : 2023-11-23 Dimitrios Spiliotopoulos, Cécile Koelbert, Marc Audebert, Ilona Barisch, Deborah Bellet, Mathilde Constans, Andreas Czich, Francis Finot, Véronique Gervais, Laure Khoury, Christian Kirchnawy, Sachiko Kitamoto, Audrey Le Tesson, Laure Malesic, Ryoko Matsuyama, Elisa Mayrhofer, Isabelle Mouche, Birgit Preikschat, Lukas Prielinger, Bernhard Rainer, Kerstin Wäse
The Ames MPF™ is a miniaturized, microplate fluctuation format of the Ames test. It is a standardized, commercially available product which can be used to assess mutagenicity in Salmonella and E. coli strains in 384-well plates using a color change-based readout. Several peer-reviewed comparisons of the Ames MPF™ to the Ames test in Petri dishes confirmed its suitability to evaluate the mutagenic potential
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Mutagenic effects of a commercial glyphosate-based herbicide formulation on the soil filamentous fungus Aspergillus nidulans depending on the mode of exposure Mutat. Res. (IF 1.9) Pub Date : 2023-10-28 Nathalie Oestreicher, Jean-Paul Bourdineaud, Christian Vélot
Glyphosate-based herbicides (GBH) are the most used pesticides worldwide. This widespread dissemination raises the question of non-target effects on a wide range of organisms, including soil micro-organisms. Despite a large body of scientific studies reporting the harmful effects of GBHs, the health and environmental safety of glyphosate and its commercial formulations remains controversial. In particular
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Saxagliptin, a selective dipeptidyl peptidase-4 inhibitor, alleviates somatic cell aneugenicity and clastogenicity in diabetic mice Mutat. Res. (IF 1.9) Pub Date : 2023-10-18 Sabry M. Attia, Sheikh F. Ahmad, Ahmed Nadeem, Mohamed S.M. Attia, Mushtaq A. Ansari, Abdelkader E. Ashour, Norah A. Albekairi, Mohammed A. Al-Hamamah, Ali A. Alshamrani, Saleh A. Bakheet
Diabetes-related complications are becoming increasingly common as the global prevalence of diabetes increases. Diabetes is also linked to a high risk of developing cancer. This raises the question of whether cancer vulnerability is caused by diabetes itself or the use of antidiabetic drugs. Chromosomal instability, a source of genetic modification involving either an altered chromosomal number or
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Could alder buckthorn (Frangula alnus Mill) be a source of chemotherapeutics effective against hepato- and colorectal carcinoma? An in vitro study Mutat. Res. (IF 1.9) Pub Date : 2023-10-14 Stefana Vuletić, Marina Bekić, Sergej Tomić, Biljana Nikolić, Stefana Cvetković, Tea Ganić, Dragana Mitić-Ćulafić
Among numerous types of cancer, hepatocellular and colorectal carcinoma are important causes of mortality. Given the nature of these cancer types and their resistance, it is of great importance to find new chemotherapeutics and therapy targets, so plant products seem to be an excellent choice in such search. The main goal of this study was to investigate anticancer activity of Frangula alnus ethyl-acetate
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Nanoplastics from ground polyethylene terephthalate food containers: Genotoxicity in human lung epithelial A549 cells Mutat. Res. (IF 1.9) Pub Date : 2023-10-10 Mohammad Alzaben, Regina Burve, Katrin Loeschner, Peter Møller, Martin Roursgaard
The ubiquitous pollution of plastic particles in most environmental matrices leads to concern about any potential adverse effects on human health. Most studies on the toxicological effect of nanoplastics has focused on standard particles of polystyrene. In reality humans are exposed to a large variety of different types and sizes of plastic material via oral intake and inhalation. In this study, we
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Hazard assessment of antineoplastic drugs and metabolites using cytotoxicity and genotoxicity assays Mutat. Res. (IF 1.9) Pub Date : 2023-10-05 Mariana de Oliveira Klein, Luiza Flavia Veiga Francisco, Izabela Natália Faria Gomes, Sergio V. Serrano, Rui M. Reis, Henrique C.S. Silveira
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Sulfoquinovosyl acylpropanediol (SQAP): Inhibition of poly(ADP-ribose) metabolism and enhanced cytotoxicity in homologous recombination repair-deficient Chinese hamster-derived cells Mutat. Res. (IF 1.9) Pub Date : 2023-09-30 Junko Maeda, Kaitlyn D. Shellenberger, Wataru Kurihara, Tomohiro Haga, Takamitsu A. Kato
Sulfoquinovosyl acylpropanediol (SQAP; a synthetic derivative of the sulfoglycolipid natural product sulfoquinovosyl acylglycerol, SQAG), has anti-tumor and radiosensitizing activities in tumor xenograft mouse models. Here, we have studied the PARP inhibitory activity of SQAP and synthetic lethality in BRCA2-deficient cells. In initial screening studies with DNA repair-deficient Chinese hamster ovary
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Evidence of genotoxicity, neurotoxicity, and antioxidant imbalance in silver catfish Rhamdia quelen after subchronic exposure to diisopentyl phthalate Mutat. Res. (IF 1.9) Pub Date : 2023-09-28 Laís Fernanda Oya-Silva, Izonete Cristina Guiloski, Taynah Vicari, Bruna Deda, Fellip Rodrigues Marcondes, Rafael Dias Simeoni, Maiara Carolina Perussolo, Anderson Joel Martino-Andrade, Daniela Morais Leme, Helena Cristina Silva de Assis, Marta Margarete Cestari
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Myricetin causes site-specific DNA damage via reactive oxygen species generation by redox interactions with copper ions Mutat. Res. (IF 1.9) Pub Date : 2023-09-18 Yuichiro Hirao, Hatasu Kobayashi, Yurie Mori, Shinya Kato, Shosuke Kawanishi, Mariko Murata, Shinji Oikawa
Myricetin (MYR), found in tea and berries, may have preventive effects on diseases, including Alzheimer’s disease and cancer. However, MYR is also a mutagen, inducing DNA damage in the presence of metal ions. We have studied the molecular mechanisms of DNA damage by MYR in the presence of Cu(II) (MYR+Cu). Using ³²P-5′-end-labeled DNA fragments, we analyzed site-specific DNA damage caused by MYR+Cu
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The calculation of historical control limits in toxicology: Do's, don'ts and open issues from a statistical perspective Mutat. Res. (IF 1.9) Pub Date : 2023-09-18 Max Menssen
For reporting toxicology studies, the presentation of historical control data and the validation of the concurrent control group with respect to historical control limits have become requirements. However, many regulatory guidelines fail to define how such limits should be calculated and what kind of target value(s) they should cover. Hence, this manuscript is aimed to give a brief review on the methods
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Whole-genome high-fidelity sequencing: A novel approach to detecting and characterization of mutagenicity in vivo Mutat. Res. (IF 1.9) Pub Date : 2023-09-09 Vasily N. Dobrovolsky, Tomonari Matsuda, Page McKinzie, Jaime Miranda, Javier R. Revollo
Direct DNA sequencing can be used for characterizing mutagenicity in simple and complex biological models. Recently we described a method of whole-genome sequencing for detecting mutations in simple models of cultured bacteria, mammalian cells, and nematode. In the current proof-of-concept study, we expand and improve our method for evaluating a more complex mammalian biological model in outbred mice
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DNA damage in foundry workers using non-invasive micronucleus cytome assay Mutat. Res. (IF 1.9) Pub Date : 2023-09-09 Hakimeh Nazari Khuniqi, Yahya Rasoulzadeh, Yousef Mohammadian
Workers in the foundry industry are exposed to hazardous chemical agents such as metal fumes, gases, vapor of molten metal, and respirable dust and hazardous physical agents such as heat, noise, and electromagnetic fields. Co-exposures to hazardous physical and chemical agents in foundry workplaces may cause DNA damage in workers. This study aimed to evaluate DNA damage in foundry workers. Thirty-three
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Quantifying telomere transcripts as tool to improve risk assessment for genetic instability and genotoxicity Mutat. Res. (IF 1.9) Pub Date : 2023-09-09 Hedwig Sutterlüty, Maximilian Bargl, Klaus Holzmann
Telomere repeat-containing RNAs (TERRA) are transcribed from telomeres as long non-coding RNAs and are part of the telomere structure with protective function. The genetic stability of cells requires telomeric repeats at the ends of chromosomes. Maintenance of telomere length (TL) is essential for proliferative capacity and chromosomal integrity. In contrast, telomere shortening is a recognized risk
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Evaluation and interpretation of cytogenetic test results based on biological relevance Mutat. Res. (IF 1.9) Pub Date : 2023-09-09 Makoto Hayashi
The evaluation and interpretation of cytogenetic test data are discussed from the perspective of biological relevance. The reliability of tests must be considered, before evaluation and interpretation. Statistical procedures are important for the evaluation of test data, but for human health risk assessment, biological relevance is essential. Cell culture conditions must be carefully considered. Cells
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Micronuclei in human peripheral blood and bone marrow as genotoxicity markers: A systematic review and meta-analysis Mutat. Res. (IF 1.9) Pub Date : 2023-09-04 Evangelia E. Antoniou, Martijn Rooseboom, Neslihan A. Kocabas, Colin M. North, Maurice P. Zeegers
Can human peripheral blood cells be used as a surrogate for bone marrow cells, in evaluating the genotoxic effects of stressors? We searched the Pubmed/Medline and PubChem databases to identify publications relevant to this question. Micronucleus formation was the genotoxicity endpoint. Three publications comparing exposed vs. non-exposed individuals are included in this analysis; the exposures were
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Assessing phytotoxicity and cyto-genotoxicity of two insecticides using a battery of in-vitro biological assays Mutat. Res. (IF 1.9) Pub Date : 2023-08-30 Mohammad Shahid, Mohammad Tarique Zeyad, Asad Syed, Ali H. Bahkali, John Pichtel, Meenakshi Verma
Intensive use of chemical pesticides in agriculture poses environmental risks and may have negative impacts on agricultural productivity. The potential phytotoxicity of two chemical pesticides, chlorpyrifos (CPS) and fensulfothion (FSN), were evaluated using Cicer arietinum and Allium cepa as model crops. Different concentrations (0–100 μgmL-1) of both CPS and FSN decreased germination and biological
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Chemical characterization of Callingcard Vine (Entada polystachya (L.) DC. var. polystachya) aqueous seed extract and evaluation of its cytotoxic, genotoxic and mutagenic properties Mutat. Res. (IF 1.9) Pub Date : 2023-08-27 Juliane Cristina Bugs de Carvalho, Iuri Marques de Oliveira, Cristiano Trindade, André Luiz Mendes Juchem, Miriana da Silva Machado, Temenouga Nikolova Guecheva, Sidnei Moura, Luiz Augusto Gomes de Souza, Marilene Henning Vainstein, João Antonio Pêgas Henriques
Callingcard Vine (Entada polystachya (L.) DC. var. polystachya - Fabaceae) is a common plant in coastal thickets from western Mexico through Central America to Colombia and Brazil, especially in Amazon biome. It has been popularly used as a urinary burning reliever and diuretic. However, the plant chemical constituents are poorly understood and Entada spp. genotoxic potential have not been previously
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Comparison of the transgenic rodent mutation assay, error corrected next generation duplex sequencing, and the alkaline comet assay to detect dose-related mutations following exposure to N-nitrosodiethylamine Mutat. Res. (IF 1.9) Pub Date : 2023-08-24 Joel P. Bercu, Shaofei Zhang, Zhanna Sobol, Patricia A. Escobar, Phu Van, Maik Schuler
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Cytotoxic and genotoxic profiles of the pyrethroid insecticide lambda-cyhalothrin and its microformulation Karate® in CHO-K1 cells Mutat. Res. (IF 1.9) Pub Date : 2023-08-18 Milagros R.R. Laborde, Marcelo L. Larramendy, Sonia Soloneski
Lambda-cyhalothrin (LCT) and its microformulation Karate® (25 % a.i.) were analysed for its genotoxicity and cytotoxicity on Chinese hamster ovary (CHO-K1) cells. Cytokinesis-block micronucleus cytome (CBMN-cyt) and alkaline single-cell gel electrophoresis (SCGE) bioassays were selected to test genotoxicity. Neutral red uptake (NRU), succinic dehydrogenase activity (MTT) and apoptogenic induction were
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In silico prediction of the mutagenicity of nitroaromatic compounds using correlation weights of fragments of local symmetry Mutat. Res. (IF 1.9) Pub Date : 2023-08-18 Andrey A. Toropov, Alla P. Toropova, Alessandra Roncaglioni, Emilio Benfenati
Most quantitative structure-property/activity relationships (QSPRs/QSARs) techniques involve using different programs separately for generating molecular descriptors and separately for building models based on available descriptors. Here, the capabilities of the CORAL program are evaluated. A user of the program should apply as the basis for models the representation of the molecular structure by means
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Alteration of genome-wide DNA methylation in non-uranium miners induced by high level radon exposure Mutat. Res. (IF 1.9) Pub Date : 2023-08-19 Pinhua Zhang, Yunyun Wu, Chunnan Piao, Yanchao Song, Yanfang Zhao, Yumin Lyu, Quanfu Sun, Jianxiang Liu
In China, according to statistics about underground non-uranium mine radon levels, 15% exceed the national standard intervention level of 1000 Bq/m3, and some mines may exceed 10,000 Bq/m3. The relationship between radon exposure in underground miners and lung cancer has already been established, but the mechanisms and biological processes underlying it are poorly understood. In order to identify the
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Blood molecular profile to predict genotoxicity from exposure to antineoplastic drugs Mutat. Res. (IF 1.9) Pub Date : 2023-08-18 Carina Ladeira, Rúben Araújo, Luís Ramalhete, Hélder Teixeira, Cecília R.C. Calado
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DNA integrity under alkaline conditions: An investigation of factors affecting the comet assay Mutat. Res. (IF 1.9) Pub Date : 2023-08-11 Erik Bivehed, Björn Hellman, Yuting Fan, Jakob Haglöf, Sonja Buratovic
The effect of pH on DNA integrity was assessed using a three-step approach. The comet assay was used on a whole genome level, with three different protocols: neutral (no alkaline unwinding), flash (pH 12.5 with 2.5 min unwinding), and the conventional alkaline protocol (pH>13 with 40 min unwinding). Real-time quantitative PCR (RT-qPCR) was then used to study the isolated DNA, revealing that gene amplification
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POT1 involved in telomeric DNA damage repair and genomic stability of cervical cancer cells in response to radiation Mutat. Res. (IF 1.9) Pub Date : 2023-08-07 Qian Li, Xiaofei Wang, Jie Liu, Lijun Wu, Shengmin Xu
Though telomeres play a crucial role in maintaining genomic stability in cancer cells and have emerged as attractive therapeutic targets in anticancer therapy, the relationship between telomere dysfunction and genomic instability induced by irradiation is still unclear. In this study, we identified that protection of telomeres 1 (POT1), a single-stranded DNA (ssDNA)-binding protein, was upregulated
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Adopting duplex sequencing technology for genetic toxicity testing: A proof-of-concept mutagenesis experiment with N-ethyl-N-nitrosourea (ENU)-exposed rats Mutat. Res. (IF 1.9) Pub Date : 2023-08-03 Stephanie L. Smith-Roe, Cheryl A. Hobbs, Victoria Hull, J. Todd Auman, Leslie Recio, Michael A. Streicker, Miriam V. Rivas, Gabriel A. Pratt, Fang Yin Lo, Jacob E. Higgins, Elizabeth K. Schmidt, Lindsey N. Williams, Daniela Nachmanson, Charles C. Valentine III, Jesse J. Salk, Kristine L. Witt
Duplex sequencing (DS) is an error-corrected next-generation sequencing method in which molecular barcodes informatically link PCR-copies back to their source DNA strands, enabling computational removal of errors in consensus sequences. The resulting background of less than one artifactual mutation per 107 nucleotides allows for direct detection of somatic mutations. TwinStrand Biosciences, Inc. has
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Relative mutagenic effectiveness and efficiency of chemical mutagens (Caffeine and EMS) and heavy metals [(Pb(NO3)2 and Cd(NO3)2)] in developing chlorophyll and morphological mutants in lentil Mutat. Res. (IF 1.9) Pub Date : 2023-07-24 Durre Shahwar, Zeba Khan, Mohammad Yunus Khalil Ansari, Younghoon Park
Mutagenic effectiveness and efficiency are the utmost vital indices to determine the effective and efficient mutagenic concentrations for the generation of high frequency of desirable mutation in mutation breeding. Nevertheless, there are meager study are available for employing effective and efficient concentration of caffeine, EMS, Pb(NO3)2 and Cd(NO3)2 for the crop improvement. Thus, the current
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Protective effect of quercetin and thymoquinone against genotoxicity and oxidative stress induced by ZnO nanoparticles in the Wistar rat model Mutat. Res. (IF 1.9) Pub Date : 2023-07-24 Nuzhat Parveen, Mohammad Abdulkader Akbarsha, A.B. Latif Wani, Mohd Owais Ansari, Md Fahim Ahmad, G.G.H.A. Shadab
Zinc oxide nanoparticles (ZnO-NPs) are increasingly used in a variety of consumer and other commercial products. Hence, man faces the risk of exposure to ZnO-NPs and the consequent adverse health effects. Mitigation/prevention of such effects using natural products has drawn the attention of scientists. Therefore, the aim of the present study has been to find the toxic effects associated with exposure
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Micronuclei and nuclear buds in amniotic tissue of rats treated with cyclophosphamide Mutat. Res. (IF 1.9) Pub Date : 2023-07-13
Fetal development can be altered by DNA damage caused by maternal exposure to chemical, physical, or biological agents during gestation. One method of assessing genotoxicity is to detect micronuclei (MNs) and/or nuclear abnormalities. This can be performed in vivo and requires only frequently dividing tissues, such as amniotic tissue (AT), which is in contact with the fetal environment and is composed
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The moderating role of macronutrient intake in relation to body composition and genotoxicity: A study with gym users Mutat. Res. (IF 1.9) Pub Date : 2023-07-18 Diene da Silva Schlickmann, Patrícia Molz, Gabriela Cristina Uebel, Caroline Santos, Caroline Brand, Renato Alberto Weber Colombelli, Thalia Gama da Silva, Juliana Priebe Steffens, Eduarda da Silva Limberger Castilhos, Pedro J. Benito, Alexandre Rieger, Silvia Isabel Rech Franke
In a cross-sectional study of gymnasium users (both sexes, ages = 41.9 ± 14.8 years), we examined the moderating role of macronutrient intake in relation to body composition and genotoxicity. A questionnaire was administered to evaluate characteristics of the participants. To assess macronutrient consumption, we used 24-h food recalls on three non-consecutive days. Body composition (body fat percentage
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Hepatocyte proliferation activity in untreated rats, measured by immunohistochemical detection of Ki-67: The effect of age on the repeated-dose liver micronucleus assay Mutat. Res. (IF 1.9) Pub Date : 2023-07-10 Kensuke Satomoto, Moeko Aoki, Atsushi Wakita, Hiroshi Yamagata, Tatsuya Mitsumoto, Takezo Okamoto, Ryoko Harada, Shuichi Hamada
The repeated-dose liver micronucleus (RDLMN) assay is a widely accepted method for detecting genotoxic substances. We investigated the effect of animal age on this assay. Proliferation activity in the liver tissue of untreated rats at age = 3.5, 6, 8, 10, or 12 weeks was measured via immunohistochemical expression of Ki-67 protein. The percentage of Ki-67-positive hepatocytes decreased markedly with
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Paraoxon and glyphosate induce DNA double-strand breaks but are not type II topoisomerase poisons Mutat. Res. (IF 1.9) Pub Date : 2023-07-06 Regina Montero-Montoya, Karen Suárez-Larios, Luis Serrano-García
We tested the hypothesis that the pesticides paraoxon and glyphosate cause DNA double-strand breaks (DSB) by poisoning the enzyme Type II topoisomerase (topo II). Peripheral lymphocytes in G0 phase, treated with the pesticides, plus or minus ICRF-187, an inhibitor of Topo II, were stimulated to proliferate; induced cytogenetic damage was measured. Micronuclei, chromatin buds, nucleoplasmic bridges
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Cytotoxicity and genotoxicity of tributyltin in the early embryonic chick, Gallus gallus domesticus Mutat. Res. (IF 1.9) Pub Date : 2023-07-01 Abhijit Mandal, Malaya Ghosh, Doli Talukdar, Pubali Dey, Aparajita Das, Sarbani Giri
Tributyltin (TBT) is used in many commercial applications, including pesticides and antifouling paints, due to its biocidal properties. We examined the cytotoxicity and genotoxicity of TBT in the early chick embryo (Gallus gallus domesticus). Chick embryos (11 days) were treated with various doses of TBT to measure LD50 values for 24, 48, and 72 h exposures, which were determined to be 110, 54, and
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Oxidative stress and DNA damage in critically ill patients with sepsis Mutat. Res. (IF 1.9) Pub Date : 2023-06-30 Olgica Mihaljevic, Snezana Zivancevic-Simonovic, Danijela Jovanovic, Svetlana Miletic Drakulic, Jovana Tubic Vukajlovic, Aleksandra Markovic, Marijana Stanojevic Pirkovic, Ivan Srejovic, Vladimir Jakovljevic, Olivera Milosevic-Djordjevic
The aim of our study was to assess the oxidative stress and inflammatory status in critically ill patients with sepsis as well as their relationship with the level of DNA damage. The study also evaluated the influence of all analyzed parameters on the outcome of the patients. The study included 27 critically ill patients with sepsis and 20 healthy subjects. Comet Assay was used for the measurement
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Allium cepa tests: A plant-based tool for the early evaluation of toxicity and genotoxicity of newly synthetized antifungal molecules Mutat. Res. (IF 1.9) Pub Date : 2023-06-29 Carlotta Alias, Donatella Feretti, Gaia V.C. Viola, Ilaria Zerbini, Franco Bisceglie, Giorgio Pelosi, Claudia Zani
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Assessment of radiation doses and DNA damage in pediatric patients undergoing interventional procedures for vascular anomalies Mutat. Res. (IF 1.9) Pub Date : 2023-06-24 Ya Ma, Lei Guo, Lianying Fang, Dianjun Hou, Rui Chen, Xiaoshan Wang, Xuesong Mao, Zihan Zhao, Yingmin Chen
Interventional procedures (IPs) have been widely used to treat vascular anomalies (VA) in recent years. However, patients are exposed to low-dose X-ray ionizing radiation (IR) during these fluoroscopy-guided IPs. We collected clinical information and IR doses during IPs and measured biomarkers including γ-H2AX, chromosome aberrations (CA), and micronuclei (MN), which underpin radiation-induced DNA
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Oxidative genomic damage in humans exposed to high indoor radon levels in Northeast Brazil Mutat. Res. (IF 1.9) Pub Date : 2023-06-23 Luíza Araújo da Costa Xavier, Julio Alejandro Navoni, Viviane Souza do Amaral
Radon gas inhalation is the main source of exposure to ionizing radiation by humans. There is still lack in knowledge concerning the chronic and indirect effects of exposure to this carcinogenic factor. Therefore, the aim of this work is to analyze the levels of oxidative genomic damage in inhabitants of a medium-high background radiation area (HBRA) (N = 82) in Northeastern Brazil and compare them
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Mesenchymal stem cell-derived exosomes attenuate DNA damage response induced by cisplatin and bleomycin Mutat. Res. (IF 1.9) Pub Date : 2023-06-08 Xiaoqiang Hu, Chuncao He, Lijun Zhang, Yunheng Zhang, Liangjing Chen, Chuan Sun, Jun Wei, Lei Yang, Xiaohua Tan, Jun Yang, Yan Zhang
Stem cell-derived exosomes (SC-Exos) have been shown to protect cells from chemical-induced deoxyribonucleic acid (DNA) damage. However, there has been no systematic comparison of the efficacy of exosomes against different types of DNA damage. Therefore, in this study, we assessed the protective effect of exosomes derived from human embryonic stem cell-induced mesenchymal stem cells (hESC-MSC-Exos)
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The small molecule Erk1/2 signaling pathway inhibitor PD98059 improves DNA repair in an experimental autoimmune encephalomyelitis SJL/J mouse model of multiple sclerosis Mutat. Res. (IF 1.9) Pub Date : 2023-06-03 S.M. Attia, S.F. Ahmad, A. Nadeem, M.S.M. Attia, M.A. Ansari, N.B. Alsaleh, A.F. Alasmari, M.A. Al-Hamamah, A. Alanazi, A.A. Alshamrani, S.A. Bakheet, G.I. Harisa
Multiple sclerosis (MS) is a demyelinating disorder in which the myelin sheath covering the central nervous system axons is damaged or lost, disrupting action potential conduction and leading to various neurological complications. The pathogenesis of MS remains unclear, and no effective therapies are currently available. MS is triggered by environmental factors in genetically susceptible individuals
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Error-corrected duplex sequencing enables direct detection and quantification of mutations in human TK6 cells with strong inter-laboratory consistency Mutat. Res. (IF 1.9) Pub Date : 2023-05-20 Eunnara Cho, Carol D. Swartz, Andrew Williams, Miriam Rivas, Leslie Recio, Kristine L. Witt, Elizabeth K. Schmidt, Jeffry Yaplee, Thomas H. Smith, Phu Van, Fang Yin Lo, Charles C. Valentine, Jesse J. Salk, Francesco Marchetti, Stephanie L. Smith-Roe, Carole L. Yauk
Error-corrected Duplex Sequencing (DuplexSeq) enables direct quantification of low-frequency mutations and offers tremendous potential for chemical mutagenicity assessment. We investigated the utility of DuplexSeq to quantify induced mutation frequency (MF) and spectrum in human lymphoblastoid TK6 cells exposed to a prototypical DNA alkylating agent, N-ethyl-N-nitrosourea (ENU). Furthermore, we explored
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Methylated and ethylated dialkylphosphate metabolites of organophosphate pesticides: DNA damage in bone marrow cells of Balb/c mice Mutat. Res. (IF 1.9) Pub Date : 2023-05-06 David Sebastián Hernandez-Toledano, Andrea Ixtchel Salazar-Osorio, Dunia Margarita Medina-Buelvas, Jessica Romero-Martínez, Elizabet Estrada-Muñiz, Libia Vega
Dialkylphosphates (DAPs), metabolites of organophosphate (OP) pesticides, are widely distributed in the environment and are often used as biomarkers of OP exposure. Recent reports indicate that DAPs may be genotoxic, both in vitro and in vivo. We have examined the genotoxicity of the methylated DAPs dimethyldithiophosphate (DMDTP) and dimethylphosphate (DMTP) and the ethylated DAPs diethyldithiophosphate
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Mycobacterium tuberculosis infection and cytogenetic abnormalities among people with HIV Mutat. Res. (IF 1.9) Pub Date : 2023-04-28 Joseph Baruch Baluku, Sharon Namiiro, Brenda Namanda, Shamim Katusabe, Dinah Namusoke, Reagan Nkonge, Tonny Okecha, Carol Nassaazi, Nixon Niyonzima, Naghib Bogere, Edwin Nuwagira, Martin Nabwana, Phillip Ssekamatte, Irene Andia-Biraro, William Worodria, Robert Salata, Sayoki Mfinanga, Stanton Gerson, Bruce Kirenga
Objective To compare cytogenetic abnormalities among people living with HIV (PLWH) with and without previous exposure to Mycobacterium tuberculosis (Mtb) (both latent tuberculosis infection [LTBI] and active tuberculosis [TB]). Methods Adult PLWH (≥18 years) were randomly selected at three HIV clinics in Uganda. Previous active TB was confirmed in the clinics’ TB records. LTBI was defined as a positive
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Low-dose ionizing radiation: Effects on the proliferation and migration of lens epithelial cells via activation of the Wnt/β-catenin pathway Mutat. Res. (IF 1.9) Pub Date : 2023-04-20 Ping Wang, Yu-Wen Li, Xue Lu, Ya Liu, Xue-Lei Tian, Ling Gao, Qing-Jie Liu, Li Fan, Mei Tian
Eye lens opacification (cataract) induced by ionizing radiation is an important concern for radiation protection. Human lens epithelial cells (HLE-B3) were irradiated with γ-rays and radiation effects, including cell proliferation, cell migration, cell cycle distribution, and other changes related to the β-catenin pathway, were determined after 8–72 h and 7 d. In an in vivo model, mice were irradiated;
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A panel of visual bacterial biosensors for the rapid detection of genotoxic and oxidative damage: A proof of concept study Mutat. Res. (IF 1.9) Pub Date : 2023-04-23 Chang-ye Hui, Shun-yu Hu, Xue-qin Yang, Yan Guo