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Piperonyl butoxide, a synergist of pesticides can elicit male-mediated reproductive toxicity Reprod. Toxicol. (IF 3.121) Pub Date : 2021-01-27 Jeong-Won Bae; Woo-Sung Kwon
A semi-synthetic methylenedioxyphenyl compound piperonyl butoxide (PBO) has been used as a ubiquitous synergist to increase the insecticidal effect of pesticides for agricultural and household use. Despite previously demonstrated effects of PBO, the detailed mechanism of PBO in spermatozoa and reproductive toxic effects on male germ cells have not been fully elucidated. Therefore, this study evaluated
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Food grade safflower concentrate: no evidence for reproduction and early developmental toxicity Reprod. Toxicol. (IF 3.121) Pub Date : 2021-01-27 Geertje Lewin; Madeleine Joel; Birgit Peter; Manfred Lützow
Safflower (Carthamus tinctorius) petals have been used for centuries as a spice, in tea blends and in traditional Asian medicine. Aqueous extracts of Safflower petals have been used as a colouring food over the last 30 years due to their bright colour. Publications in the past raised concerns about fertility impairing, maternal toxicity, fetotoxic and teratogenic properties in rodents. As the tested
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PARENTAL EXPOSURE TO BENZO(A)PYRENE IN THE PERIPUBERTAL PERIOD IMPACTS REPRODUCTIVE ASPECTS OF THE F1 GENERATION IN RATS Reprod. Toxicol. (IF 3.121) Pub Date : 2021-01-26 Bárbara Campos Jorge; Ana Carolina Casali Reis; Julia Stein; Paola da Silva Balin; Érika Tissiana Sterde; Mariana Gazoli Barbosa; Ariana Musa de Aquino; Cândida Aparecida Leite Kassuya; Arielle Cristina Arena
Benzo(a)pyrene (BaP) is an ubiquitous environmental pollutant which can lead to adverse effects on male reproduction. However, the persistence of these changes on a multigenerational scale has not been sufficiently explored. This study evaluated if peripubertal exposure to BaP in male rats can induce reproductive impairment in offspring. Male rats received BaP at environmentally relevant doses (0,
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A human relevant mixture of persistent organic pollutants (POPs) and perfluorooctane sulfonic acid (PFOS) differentially affect glutamate induced excitotoxic responses in chicken cerebellum granule neurons (CGNs) in vitro Reprod. Toxicol. (IF 3.121) Pub Date : 2021-01-23 Ajay Yadav; Steven Verhaegen; Evelien Verbruggen; Marie Kerhoas; Eva Henriette Willemijn Huiberts; Mussie Ghezu Hadera; Hanne Friis Berntsen; Karin Elisabeth Zimmer; Erik Ropstad; Ragnhild Elisabeth Paulsen
Primary cultures of cerebellar granule neurons (CGNs) derived from chicken embryos were used to explore the effects on developmental neurotoxicity by a complex defined mixture of persistent organic pollutants (POPs). Its chemical composition and concentrations were based on blood levels in the Norwegian/Scandinavian population. Perfluorooctane sulfonic acid (PFOS) alone, its most abundant compound
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RADIOFREQUENCY RADIATION: A POSSIBLE THREAT TO MALE FERTILITY Reprod. Toxicol. (IF 3.121) Pub Date : 2021-01-23 Himanshi; Umesh Rai; Rajeev Singh
Radiofrequency exposure from man-made sources has increased drastically with the era of advanced technology. People could not escape from such RF radiations as they have become the essential part of our routine life such as Wi-Fi, microwave ovens, TV, mobile phones, etc. Although non-ionizing radiations are less damaging than ionizing radiations but its long term exposure effect cannot be avoided.
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A pilot study: Relationship between Bisphenol A, Bisphenol A glucuronide and sex steroid hormone levels in cord blood in A South African population Reprod. Toxicol. (IF 3.121) Pub Date : 2021-01-13 Verena Gounden; Mohamed Zain Warasally; Thabo Magwai; Rajen Naidoo; Anil Chuturgoon
Exposure to Bisphenol A (BPA) during early development particularly in- utero has been linked to a wide range of pathology. The aim of this study was to examine the relationship of BPA and its naturally occurring metabolite BPA-glucuronide (BPA-g) with sex steroid hormone levels in South African mother-child pairs. Third-trimester serum maternal samples and matching cord blood samples were analyzed
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Panel of suitable reference genes and its gender differences of fetal rat liver under physiological conditions and exposure to dexamethasone during pregnancy Reprod. Toxicol. (IF 3.121) Pub Date : 2021-01-14 Heze Liu; Liang Liu; Hui Han; Kexin Liu; Hui Wang
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Macrolide and lincosamide antibiotic exposure in the first trimester of pregnancy and risk of congenital anomaly: A European case-control study Reprod. Toxicol. (IF 3.121) Pub Date : 2021-01-14 Aminkeng Zawuo Leke; Helen Dolk; Maria Loane; Karen Casson; Vera Nelen; Ingeborg Barišić; Ester Garne; Anke Rissman; Mary O’Mahony; Amanda J Neville; Anna Pierini; Jorieke E.H. Bergman; Kari Klungsøyr; Anna Materna-Kiryluk; Anna Latos Bielenska; Clara Cavero Carbonell; Marie-Claude Addor; David Tucker
This study investigated the risk of congenital heart defects (CHD) and other congenital anomalies (CA) associated with first trimester use of macrolide antibiotics (mainly erythromycin, spiramycin, clarithromycin and azithromycin) and lincosamides (clindamycin) using a case-malformed control design. Data included 145,936 babies with a CA diagnosis (livebirths, stillbirths and terminations of pregnancy
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Cadmium exposure reduces invasion of the human trophoblast-derived HTR-8/SVneo cells by inhibiting cell adhesion and matrix metalloproteinase-9 secretion Reprod. Toxicol. (IF 3.121) Pub Date : 2021-01-07 Laura Diaz-Cueto; Pablo Dominguez-Lopez; Lucero Paniagua; Rebeca Martinez-Quezada; Fabian Arechavaleta-Velasco
Preeclampsia and intrauterine growth restriction, multisystemic disorders characterized by a shallow trophoblast invasion, have been associated with maternal cadmium (Cd) exposure. The molecular mechanisms of this association remain unknown. Cell adhesion and matrix metalloproteinase production are essential for an adequate trophoblast invasion. Thus, the aim of this study was to determine the effect
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Assessment of chemical mixtures using biomarkers of combined biological activity: A screening study in human placentas Reprod. Toxicol. (IF 3.121) Pub Date : 2021-01-11 Andrea Rodríguez-Carrillo; Anna Kjerstine Rosenmai; Vicente Mustieles; Stephan Couderq; Jean-Baptiste Fini; Fernando Vela-Soria; Jose Manuel Molina-Molina; Patricia Ferrando-Marco; Maria Wielsøe; Manhai Long; Eva Cecilie Bonefeld-Jorgensen; Nicolás Olea; Anne Marie Vinggaard; Mariana F. Fernández
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Neither soy nor isoflavone intake affects male reproductive hormones: An expanded and updated meta-analysis of clinical studies Reprod. Toxicol. (IF 3.121) Pub Date : 2020-12-28 Katharine E. Reed; Juliana Camargo; Jill Hamilton-Reeves; Mindy Kurzer; Mark Messina
Concerns that the phytoestrogens (isoflavones) in soy may feminize men continue to be raised. Several studies and case-reports describing feminizing effects including lowering testosterone levels and raising estrogen levels in men have been published. For this reason, the clinical data were meta-analyzed to determine whether soy or isoflavone intake affects total testosterone (TT), free testosterone
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Cyclic depsipeptide mycotoxin exposure may cause human endocrine disruption: Evidence from OECD in vitro stably transfected transcriptional activation assays Reprod. Toxicol. (IF 3.121) Pub Date : 2020-12-17 Yooheon Park; Hee-Seok Lee
The presence of cyclic depsipeptide mycotoxins in foods and feedstuffs could potentially cause endocrine disrupting effects on humans and wildlife by their inhibition of active steroidogenesis. Therefore, we attempted to assess the human estrogen receptor (ER) and androgen receptor (AR) agonistic/antagonistic effects of representative cyclic depsipeptide mycotoxins, enniatin A1 (ENN A1), and enniatin
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Maternal use of fluconazole and congenital malformations in the progeny: A meta-analysis of the literature Reprod. Toxicol. (IF 3.121) Pub Date : 2020-12-28 Maria Cristina Budani; Stefania Fensore; Marco Di Marzio; Gian Mario Tiboni
Fluconazole is a bis-triazole agent used in the treatment of superficial and systemic fungal infections, with vaginal candidiasis being one of the commonest indications to fluconazole treatment. There is increasing concern regarding the teratogenic potential of fluconazole. The aim of this meta-analysis is to pool the literature data in order to evaluate the possible association between fluconazole
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Oxidative stress in retrotrapezoid nucleus/parafacial respiratory group and impairment of central chemoreception in rat offspring exposed to maternal cigarette smoke Reprod. Toxicol. (IF 3.121) Pub Date : 2020-12-28 Fang Lei; Wen Wang; Yating Fu; Ji Wang; Yu Zheng
We have reported that smoking during pregnancy is associated with deficit in neonatal central chemoreception. However, the underlying mechanism is not well clarified. In this study, we developed a rat model of maternal cigarette smoke (CS) exposure. Pregnant rats were exposed to CS during gestational day 1−20. Offspring were studied on postnatal day 2. Reactive oxygen species (ROS) content and expressions
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Exposure to human relevant mixtures of halogenated persistent organic pollutants (POPs) alters neurodevelopmental processes in human neural stem cells undergoing differentiation Reprod. Toxicol. (IF 3.121) Pub Date : 2020-12-14 Nichlas Davidsen; Anna Jacobsen Lauvås; Oddvar Myhre; Erik Ropstad; Donatella Carpi; Emilio Mendoza-de Gyves; Hanne Friis Berntsen; Hubert Dirven; Ragnhild E Paulsen; Anna Bal-Price; Francesca Pistollato
Halogenated persistent organic pollutants (POPs) like perfluorinated alkylated substances (PFASs), brominated flame retardants (BFRs), organochlorine pesticides and polychlorinated biphenyls (PCBs) are known to cause cancer, immunotoxicity, neurotoxicity and interfere with reproduction and development. Concerns have been raised about the impact of POPs upon brain development and possibly neurodevelopmental
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Paraben exposure alters cell cycle progression and survival of spontaneously immortalized secretory murine oviductal epithelial (MOE) cells Reprod. Toxicol. (IF 3.121) Pub Date : 2020-12-24 Ayelet Ziv-Gal; Malia D. Berg; Matthew Dean
The mammalian oviduct is a central organ for female reproduction as it is the site of fertilization and it actively transports the embryo to the uterus. The oviduct is responsive to ovarian steroids and thus, it is a potential target of endocrine disrupting chemicals. Parabens are antimicrobial compounds that are prevalently found in daily-used products. However, recent studies suggest that some parabens
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Host microRNAs regulate expression of hepatitis B virus genes during transmission from patients’ sperm to embryo Reprod. Toxicol. (IF 3.121) Pub Date : 2020-12-15 Ji-Hua Huang; Ting-Ting Han; Ling-Xiao Li; Ting Qu; Xin-Yue Zhang; Xue Liao; Ying Zhong
Human sperm nucleus contains diverse RNA populations. This study aimed to screen and identify host microRNAs (miRs) that regulate gene expression of hepatitis B virus (HBV) during transmission from patients’ sperm to sperm-derived embryos. Using microarrays, 336 miRs were found to be differentially expressed. After validation using real-time quantitative RT–PCR (RT-qPCR), four miRs were selected as
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Effects of bisphenol A and bisphenol S on microRNA expression during bovine (Bos taurus) oocyte maturation and early embryo development Reprod. Toxicol. (IF 3.121) Pub Date : 2020-12-04 Reem Sabry; Angela C. Saleh; Leanne Stalker; Jonathan LaMarre; Laura A. Favetta
Bisphenol A (BPA) and its alternative, bisphenol S (BPS), are widespread endocrine disrupting compounds linked in several studies to poor female fertility. Sufficient oocyte competence and subsequent embryo development are highly dependent on oocyte maturation, an intricate process that is vulnerable to BPA. These effects as well as the effects of its analog, BPS, have not been fully elucidated. Although
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Delay in puberty indices of Wistar rats caused by Cadmium. Focus on the redox system in reproductive organs Reprod. Toxicol. (IF 3.121) Pub Date : 2020-11-26 Joel Hernández-Rodríguez; Ana Laura López; Sergio Montes; Herlinda Bonilla-Jaime; Ivis Morales; Ofelia Limón-Morales; Camilo Ríos; Marisela Hernández-González; Rosa María Vigueras-Villaseñor; Marcela Arteaga-Silva
Puberty is a transitional period from juvenile stage to adulthood, followed by the functional maturation of gonads and reproductive organs. This period is sensitive to environmental pollutants like cadmium (Cd), a heavy metal that represents a serious health risk. Cd is an endocrine disruptor that interferes with reproduction by causing oxidative stress in the reproductive organs, affecting the sexual
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Perinatal selective serotonin reuptake inhibitor (SSRI) and other antidepressant exposure effects on anxiety and depressive behaviors in offspring: A review of findings in humans and rodent models Reprod. Toxicol. (IF 3.121) Pub Date : 2020-11-27 Sarah M. Hutchison; Louise C. Mâsse; Jodi L. Pawluski; Tim F. Oberlander
The developmental impact of selective serotonin reuptake inhibitor (SSRI) and other antidepressant treatments during gestation and postpartum on anxiety and depression behaviors in offspring is unclear. This review focuses on how perinatal exposure to SSRI and other antidepressant may have long term consequences for these affective behaviors during early childhood and beyond. Outcomes vary and consideration
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SARS-COV-2 (Covid-19) and male fertility: Where are we? Reprod. Toxicol. (IF 3.121) Pub Date : 2020-11-26 Temidayo S Omolaoye; Adeloye A Adeniji; Walter D. Cardona Maya; Stefan S du Plessis
Severe acute respiratory syndrome coronavirus 2 (SARS−COV-2), a single-stranded RNA virus, was found to be the causal agent of the disease called coronavirus disease. During December 2019, China informed the World Health Organization (WHO) of an outbreak of cases of pneumonia of unknown etiology, which caused severe-acute respiratory distress. The disease was termed coronavirus disease 2019 (Covid-19)
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A pilot investigation of couple-level phthalates exposure and in vitro fertilization (IVF) outcomes Reprod. Toxicol. (IF 3.121) Pub Date : 2020-11-30 Thoin F. Begum; Victor Y. Fujimoto; Roy Gerona; Alexandra McGough; Nikolaus Lenhart; Rebecca Wong; Evelyn Mok-Lin; Jonathan Melamed; Celeste D. Butts; Michael S. Bloom
Phthalates are reproductive toxicants in experimental animal studies and exposure has been associated with infertility in human populations, although the results have been inconsistent. To help to address the data gap, we conducted a hypothesis-generating investigation of associations between urinary phthalate metabolites and reproductive outcomes among women (n = 56) and their male partners (n = 43)
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25th anniversary of the Berlin Workshop on Developmental Toxicology: DevTox database update, challenges in risk assessment of developmental neurotoxicity and alternative methodologies in bone development and growth Reprod. Toxicol. (IF 3.121) Pub Date : 2020-12-02 Philip Marx-Stoelting; Marize de L.M. Solano; Hiroaki Aoyama; Ralf H. Adams; Anna Bal-Price; Jochen Buschmann; Ibrahim Chahoud; Ruth Clark; Tian Fang; Michio Fujiwara; Michael Gelinsky; Konstanze Grote; Masao Horimoto; Susanne Hougaard Bennekou; Rupert Kellner; Makiko Kuwagata; Marcel Leist; Annemarie Lang; Rol Solecki
25 years after the first Berlin Workshop on Developmental Toxicity this 10th Berlin Workshop aimed to bring together international experts from authorities, academia and industry to consider scientific, methodologic and regulatory aspects in risk assessment of developmental toxicity and to debate alternative strategies in testing developmental effects in the future. Proposals for improvement of the
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Recognized trophoblast-like cells conversion from human embryonic stem cells by BMP4 based on convolutional neural network Reprod. Toxicol. (IF 3.121) Pub Date : 2020-11-26 Yajun Liu; Yi Zhang; Jinquan Cui
The use of models of stem cell differentiation to trophoblastic cells provides an effective perspective for understanding the early molecular events in the establishment and maintenance of human pregnancy. In combination with the newly developed deep learning technology, the automated identification of this process can greatly accelerate the contribution to relevant knowledge. Based on the transfer
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Herbicides glyphosate and glufosinate ammonium negatively affect human sperm mitochondria respiration efficiency Reprod. Toxicol. (IF 3.121) Pub Date : 2020-11-26 Alessandra Ferramosca; Stefano Lorenzetti; Mariangela Di Giacomo; Francesco Murrieri; Lamberto Coppola; Vincenzo Zara
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Prenatal developmental toxicity studies on fumes from bitumen in the rat Reprod. Toxicol. (IF 3.121) Pub Date : 2020-11-26 Peter J. Boogaard; Jochen Buschmann; Rainer Fuhst; Katharina Blümlein; Katharina Schwarz; Dirk Schaudien; Wolfgang Koch; Christine McAlinden; Lize Deferme; Mathieu Vaissiere; Hans B. Ketelslegers; Anna Steneholm
The prenatal developmental toxicity of bitumen fume was tested by nose-only inhalation in the rat. The fumes for exposure were collected from the headspace of a storage tank filled with a bitumen corresponding in composition to an anticipated worst-case occupational exposure. The composition of these fumes was compared to actual paving site fumes to ensure its representativeness for workplace exposures
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Different effects of an N-phenylimide herbicide on heme biosynthesis between human and rat erythroid cells Reprod. Toxicol. (IF 3.121) Pub Date : 2020-11-26 Satoshi Kawamura; Mitsuhiro Otani; Taiki Miyamoto; Jun Abe; Ryo Ihara; Kunifumi Inawaka; Alan G. Fantel
Rat developmental toxicity including embryolethality and teratogenicity (mainly ventricular septal defects and wavy ribs) were produced by S-53482, an N-phenylimide herbicide that inhibits protoporphyrinogen oxidase (PPO) common to chlorophyll and heme biosynthesis. The sequence of key biological events in the mode of action has been elucidated as follows: inhibition of PPO interferes with normal heme
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Pregnancy outcome following in-utero exposure to ondansetron: A prospective comparative observational study Reprod. Toxicol. (IF 3.121) Pub Date : 2020-11-16 Razan Sakran; Svetlana Shechtman; Judy Arnon; Orna Diav-Citrin
The objective of the study was to evaluate the rate of major congenital anomalies after first trimester exposure to ondansetron for nausea and vomiting of pregnancy (NVP). The design is a prospective, comparative, observational cohort study, performed at the Israeli Teratology Information Service between 2010 and 2014. Follow-up was obtained for 195 ondansetron-exposed, 110 metoclopramide-exposed,
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No Obviously Adverse Pregnancy Complications and Outcomes of the Recovered Pregnant Women from COVID-19 Reprod. Toxicol. (IF 3.121) Pub Date : 2020-11-26 Cuifang Fan; Yuping Guo; Peng Qu; Suqing Wang; Ming Wang; Jingping Yuan; Chunyan Li; Likun Gao; Yiming Pang; Zhiwen Li; Bin Wang
The effects of SARS-COV-2 infection on the pregnant women and their fetus growth have attracted worldwide concern. Our case study aimed to investigate the neonatal clinical outcomes of the recovered pregnant women from COVID-19 in China, expecting to provide the clinical references of urgent need for other countries. Our study recruited a total of 12 recovered pregnant women from COVID-19 prior to
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Hypoxia-induced GPR4 suppresses trophoblast cell migration and proliferation through the MAPK signaling pathway Reprod. Toxicol. (IF 3.121) Pub Date : 2020-11-05 Haining Qi; Cuiyun Yao; Jianhong Xing; Ying Qin
Pre-eclampsia (PE), a common pregnancy-systemic syndrome, is characterized by proteinuria and hypertension and is the leading cause of maternal and fetal mortality. Thus, we aim to investigate the role of G-Protein Coupled Receptor 4 (GPR4) in PE and the underlying molecular mechanism. In this study, GSE66273 microarray data were obtained from the Gene Expression Omnibus(GEO) database of the National
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Retinoid Signaling in Skeletal Development: Scoping the System for Predictive Toxicology Reprod. Toxicol. (IF 3.121) Pub Date : 2020-11-14 Thomas B. Knudsen; Jocylin D. Pierro; Nancy C. Baker
All-trans retinoic acid (ATRA), the biologically active form of vitamin A, is instrumental in regulating the patterning and specification of the vertebrate embryo. Various animal models demonstrate adverse developmental phenotypes following experimental retinoid depletion or excess during pregnancy. Windows of vulnerability for altered skeletal patterning coincide with early specification of the body
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Valproic acid alters nitric oxide status in neurulating mouse embryos Reprod. Toxicol. (IF 3.121) Pub Date : 2020-11-04 Gian Mario Tiboni; Adalisa Ponzano; Alessio Ferrone; Sara Franceschelli; Lorenza Speranza; Antonia Patruno
The molecular bases of the teratogenic effects elicited by valproic acid (VPA) are not fully defined. It was previously shown that inhibition of nitric oxide (NO) synthesis is associated with an enhancement of the teratogenic effects of VPA, while amplification of NO signal by sildenafil prompted a dose-dependent reduction of VPA-induced neural tube defects. In this study, for the first time, the effect
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Cell-free mitochondrial DNA increases granulosa cell apoptosis and reduces aged oocyte blastocyst development in the mouse Reprod. Toxicol. (IF 3.121) Pub Date : 2020-11-02 Yu Liu; Qiuzi Shen; Huiying Li; Wenpei Xiang; Ling Zhang
Cell-free mitochondrial DNA (cf-mtDNA) released into the extracellular environment can cause cellular inflammatory responses and damage. Here, we investigated the effects of cf-mtDNA on mouse ovarian granulosa cell function and on the developmental competence of oocytes matured in vitro. Granulosa cells in the cf-mtDNA treatment group had a lower ATP content (P < 0.05), a higher apoptotic cell percentage
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Exposure to perfluorobutane sulfonate and perfluorooctanesulfonic acid disrupts the production of angiogenesis factors and stress responses in human placental syncytiotrophoblasts Reprod. Toxicol. (IF 3.121) Pub Date : 2020-11-02 Angela Pham; Jun Zhang; Liping Feng
Poly- and per-fluoroalkyl substances (PFAS) have attracted widespread attention in recent years due to their bioaccumulation, toxicity, and ubiquitous nature. We and others have reported that maternal exposure to PFAS is associated with adverse birth outcomes due to altered placental functions. In this study, we investigated the effects of two major PFAS compounds, perfluorobutane sulfonate (PFBS)
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Focus on germ-layer markers: A human stem cell-based model for in vitro teratogenicity testing Reprod. Toxicol. (IF 3.121) Pub Date : 2020-11-02 Manuela Jaklin; Jitao David Zhang; Paul Barrow; Martin Ebeling; Nicole Clemann; Marcel Leist; Stefan Kustermann
Human induced pluripotent stem cells (hiPSC) were used to develop an assay format that may deliver information on teratogenicity of drugs. A human pluripotent stem cell scorecard panel was used to monitor the expression of 96 marker genes that are indicative of the stem cell state or differentiation into the ectoderm, mesoderm and endoderm lineages. We selected a human episomal iPS cell line for the
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Prenatal exposure to an environmentally relevant phthalate mixture accelerates biomarkers of reproductive aging in a multiple and transgenerational manner in female mice Reprod. Toxicol. (IF 3.121) Pub Date : 2020-10-28 Emily Brehm; Changqing Zhou; Liying Gao; Jodi A. Flaws
Phthalates are known endocrine-disrupting chemicals that are found in many consumer products. Our laboratory previously developed a relevant phthalate mixture consisting of six phthalates and found that it disrupted female fertility in mice. However, it is unknown if prenatal exposure to phthalate mixtures can accelerate reproductive aging and if this occurs in multiple generations. Thus, we tested
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MDR-1 function protects oocyte mitochondria against the transgenerational effects of nitrogen mustard exposure Reprod. Toxicol. (IF 3.121) Pub Date : 2020-10-23 Haley Clark; Barbara Pereira Vera; Zijing Zhang; Lynae M. Brayboy
Oocytes are vulnerable to alkylating agents like nitrogen mustard (NM), which can cause mitochondrial dysfunction associated with increased oxidative stress. Because mitochondria are maternally inherited, NM exposure affects oocyte mitochondrial physiology and compromises future progeny. Multidrug resistance transporters (MDRs) are transmembrane proteins that efflux such cytotoxic substances; MDR-1
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The optimal compound reference genes for qRT-PCR analysis in the developing rat long bones under physiological conditions and prenatal dexamethasone exposure model Reprod. Toxicol. (IF 3.121) Pub Date : 2020-10-18 Hui Han; Liang Liu; Ming Chen; Yi Liu; Hui Wang; Liaobin Chen
Quantitative real-time polymerase chain reaction (qRT-PCR) is a powerful tool for evaluating gene expression, but its accuracy is affected by the stability of the reference genes used for normalization. The Minimum Information for Publication of Quantitative Real-time PCR Experiments (MIQE) guidelines indicated that it was important to use multiple stable reference genes as compound reference genes
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Omega-3 polyunsaturated fatty acids alleviate hydrogen sulfide-induced blood-testis barrier disruption in the testes of adult mice Reprod. Toxicol. (IF 3.121) Pub Date : 2020-10-15 Shuxian Wang; Qiwei Chen; Yu Zhang; Feng Zheng; Tongmin Xue; Xie Ge; Rujun Ma; Xiaoyan Li; Ronghua Wu; Kuan Liang; Zhang Qian; Yifeng Ge; Jinzhao Ma; Bing Yao
Hydrogen sulfide (H2S), a gaseous intracellular signal transducer, participates in multiple physiological and pathological conditions, including reproductive conditions, and disrupts spermatogenesis. The blood-testis barrier (BTB) plays a vital role in spermatogenesis. However, the effect of H2S on the BTB and the underlying mechanism remain unclear. Herein, we examined the effect of H2S and omega-3
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Knockout mouse models are predictive of malformations or embryo-fetal death in drug safety evaluations Reprod. Toxicol. (IF 3.121) Pub Date : 2020-10-13 Natasha R. Catlin; Christine Stethem; Christopher J. Bowman; Sarah N. Campion; William S. Nowland; Gregg D. Cappon
Traditionally, understanding potential developmental toxicity from pharmaceutical exposures has been based on the results of ICH guideline studies in two species. However, support is growing for the use of weight of evidence approaches when communicating the risk of developmental toxicity, where the intended pharmacologic mode of action affects fundamental pathways in developmental biology or phenotypic
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Intrauterine growth restriction: clinical consequences on health and disease at adulthood Reprod. Toxicol. (IF 3.121) Pub Date : 2020-10-10 JB Armengaud; C Yzydorczyk; B Siddeek; AC Peyter; U Simeoni
Intrauterine growth restriction (IUGR) affects 10 to 15% of all pregnancies worldwide. IUGR may result from maternal, placental or fetal origin. Maternal malnutrition before and during pregnancy represents the most prevalent non-genetic or placental cause. IUGR reflects an abnormal adaptive fetal growth in a deleterious environment. Individuals born after IUGR are more susceptible to develop diseases
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Effect of Vitamin E deficiency on spermatogenesis in mice and its similarity to aging Reprod. Toxicol. (IF 3.121) Pub Date : 2020-10-10 Hirokatsu Saito; Kenshiro Hara; Satoshi Kitajima; Kentaro Tanemura
Vitamin E (VE) plays numerous important roles in mammals because of its antioxidant activity. As a result, VE deficiency (VED) leads to the dysfunction of central nervous, reproductive, and immune systems. However, few studies have reported the effects of VED on the male reproductive system. In this study, we investigated the effects of VED on male reproductive function and examined its relationship
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lncRNA involved in triptonide-induced cytotoxicity in mouse germ cells Reprod. Toxicol. (IF 3.121) Pub Date : 2020-10-09 Xiaoning Zhang; Chen Chen; Yuanyuan Zhong; Xuhui Zeng
Triptonide reportedly has strong antitumor and anti-inflammatory activities. However, its severe toxicity, including to the reproductive system, has greatly restricted its use in patients who wish to preserve fertility. lncRNAs play crucial roles in male fertility and reportedly regulate triptonide’s antitumor activity. However, whether lncRNAs are involved in triptonide-induced reproductive toxicity
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Rutin prevents cisplatin-induced ovarian damage via antioxidant activity and regulation of PTEN and FOXO3a phosphorylation in mouse model Reprod. Toxicol. (IF 3.121) Pub Date : 2020-10-05 Thae Lanne B.G. Lins; Bruna B. Gouveia; Ricássio S. Barberino; Regina L.S. Silva; Alane P.O. Monte; Joisyleide G.C. Pinto; Daniela S.P. Campinho; Raimundo C. Palheta; Maria H.T. Matos
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Identification and Verification of hub microRNAs in varicocele rats through High-throughput Sequencing and Bioinformatics Analysis Reprod. Toxicol. (IF 3.121) Pub Date : 2020-10-03 Ningjing Ou; Yuxuan Song; Yawei Xu; Yongjiao Yang; Xiaoqiang Liu
Varicocele (VC) is the most common treatable cause of infertility, but it is difficult to distinguish fertile from infertile VC populations because the pathogenesis is unclear. In order to study the related mechanism of VC causing male sterility, we made VC rats model by surgery, analyzed the rat epididymal sperm, and use the transcriptome sequencing compared all the miRNA expression differences in
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Vitamin C protects early mouse embryos against juglone toxicity Reprod. Toxicol. (IF 3.121) Pub Date : 2020-10-01 Xue Zhang; Changyin Zhou; Wenxiu Cheng; Ruixin Tao; Hongxia Xu; Honglin Liu
Juglone, a naphthoquinone isolated from many species of the Juglandaceae (walnut) family, has been used in traditional Chinese medicine for centuries for its various pharmacological effects. Our previous research found its toxic effects on oocytes maturation. But we still know a little about its toxic effects on embryo development. Here, we used mouse embryo as a model to explore the effects of juglone
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Integrating biokinetics and in vitro studies to evaluate developmental neurotoxicity induced by chlorpyrifos in human iPSC-derived neural stem cells undergoing differentiation towards neuronal and glial cells Reprod. Toxicol. (IF 3.121) Pub Date : 2020-10-01 Emma Di Consiglio; Francesca Pistollato; Emilio Mendoza-De Gyves; Anna Bal-Price; Emanuela Testai
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Effects of prepubertal exposure to forchlorfenuron through prenatal and postnatal gavage administration in developing Sprague-Dawley rats Reprod. Toxicol. (IF 3.121) Pub Date : 2020-09-28 Difeng Zhu; Li Ping; Xiaofei Shen; Yawen Hong; Qinjie Weng; Qiaojun He; Jiajia Wang; Jincheng Wang
Forchlorfenuron (CPPU), a plant growth regulator, is widely used in agriculture. However, its long-term exposure effects on humans, especially neonates, remain unclear. Therefore, we investigated the developmental toxicity of prenatal and postnatal gavage administration of CPPU in rats. Pregnant Sprague-Dawley rats were administered 300 mg/kg/day CPPU by gavage from day 6 of gestation to the cessation
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Perfluorooctane sulfonic acid (PFOS) exposure during Pregnancy Increases Blood Pressure and Impairs Vascular Relaxation Mechanisms in the Adult Offspring Reprod. Toxicol. (IF 3.121) Pub Date : 2020-09-24 Sri Vidya Dangudubiyyam; Jay S. Mishra; Hanjie Zhao; Sathish Kumar
Perfluorooctanesulfonate (PFOS) is a persistent environmental agent. We examined whether PFOS exposure during pregnancy alters blood pressure in male and female offspring, and if this is related to sex-specific changes in vascular mechanisms. PFOS was administered through drinking water (50 µg/ml) to pregnant Sprague-Dawley rats from gestational day 4 until delivery. PFOS-exposure decreased maternal
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Lead and Cadmium exposure induces male reproductive dysfunction by modulating the expression profiles of apoptotic and survival signal proteins in tea-garden workers Reprod. Toxicol. (IF 3.121) Pub Date : 2020-09-22 Sreyashi Mitra; Alex C. Varghese; Syamsundar Mandal; Sayantan Bhattacharyya; Parag Nandi; Syed M. Rahman; Kushal Kumar Kar; Rinku Saha; Shubhadeep Roychoudhury; Nabendu Murmu
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Essential trace elements in umbilical cord tissue and risk for neural tube defects Reprod. Toxicol. (IF 3.121) Pub Date : 2020-09-21 Mengyuan Liu; Lei Jin; Jinhui Yu; Zaiming Su; Ying Sun; Yaqiong Liu; Qing Xie; Zhiwen Li; Linlin Wang; Aiguo Ren
Deficient or excessive quantities of essential trace elements (ETEs)1 in the fetal environment can compromise developmental processes. We investigated whether concentrations of zinc (Zn), manganese (Mn), selenium (Se), cobalt (Co), molybdenum (Mo), and nickel (Ni) in umbilical cord tissue are associated with risk for neural tube defects (NTDs). Umbilical cord tissues from 166 cases of NTD cases and
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Perfluorooctanoic acid (PFOA) inhibits the gap junction intercellular communication and induces apoptosis in human ovarian granulosa cells Reprod. Toxicol. (IF 3.121) Pub Date : 2020-09-21 Yuanyuan Zhou; Hongping Li; Chuanping Lin; Yuchan Mao; Jinpeng Rao; Yiyun Lou; Xinyun Yang; XiangRong Xu; Fan Jin
Perfluorooctanoic acid (PFOA) has attracted widespread research attention as it is very stable, bioaccumulates, and causes reproductive toxicity. Data from several animal experiments and epidemiological studies indicate that female fertility may decline because of ovarian granulosa cell (GC) apoptosis as oocyte quality is positively associated with effective gap junctional intercellular communication
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Perinatal exposure to Bisphenol A disturbs the early differentiation of male germ cells. Reprod. Toxicol. (IF 3.121) Pub Date : 2020-09-18 Romina Pagotto,Clarisa G Santamaría,Harreguy María Belén,Julián Abud,María Laura Zenclussen,Laura Kass,Martina Crispo,Mónica M Muñoz-de-Toro,Horacio A Rodriguez,Mariela Bollati-Fogolín
Understanding the effects of Bisphenol A (BPA) on early germ cell differentiation and their consequences in adult life is an area of growing interest in the field of endocrine disruption. Herein, we investigate whether perinatal exposure to BPA affects the differentiation of male germ cells in early life using a transgenic mouse expressing the GFP reporter protein under the Oct4 promoter. In this model
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Inhalation of ammonium sulfate and ammonium nitrate adversely affect sperm function. Reprod. Toxicol. (IF 3.121) Pub Date : 2020-08-29 Jeong-Won Bae,Hong Ju Kwon,So-Hye Kim,Lei Ma,Hobin Im,Eungyung Kim,Myoung Ok Kim,Woo-Sung Kwon
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An efficient neuron-astrocyte differentiation protocol from human embryonic stem cell-derived neural progenitors to assess chemical-induced developmental neurotoxicity. Reprod. Toxicol. (IF 3.121) Pub Date : 2020-09-12 Victoria C de Leeuw,Conny T M van Oostrom,Remco H S Westerink,Aldert H Piersma,Harm J Heusinkveld,Ellen V S Hessel
Human embryonic stem cell neuronal differentiation models provide promising in vitro tools for the prediction of developmental neurotoxicity of chemicals. Such models mimic essential elements of human relevant neuronal development, including the differentiation of a variety of brain cell types and their neuronal network formation as evidenced by specific gene and protein biomarkers. However, the reproducibility
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The effects of a technical mixture of naphthenic acids on placental trophoblast cell function. Reprod. Toxicol. (IF 3.121) Pub Date : 2020-08-29 Laiba Jamshed,Sergio Raez-Villanueva,Genevieve A Perono,Philippe J Thomas,Alison C Holloway
There is considerable concern that naphthenic acids (NA) related to oil extraction can negatively impact reproduction in mammals, yet the mechanisms are unknown. Since placental dysfunction is central to many adverse pregnancy outcomes, the goal of this study was to determine the effects of NA exposure on placental trophoblast cell function. HTR-8/SVneo cells were exposed to a commercial technical
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An ontology for developmental processes and toxicities of neural tube closure. Reprod. Toxicol. (IF 3.121) Pub Date : 2020-09-11 Harm J Heusinkveld,Yvonne C M Staal,Nancy C Baker,George Daston,Thomas B Knudsen,Aldert Piersma
In recent years, the development and implementation of animal-free approaches to chemical and pharmaceutical hazard and risk assessment has taken off. Alternative approaches are being developed starting from the perspective of human biology and physiology. Neural tube closure is a vital step that occurs early in human development. Correct closure of the neural tube depends on a complex interplay between
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Concentrations of organochlorine pesticides in placental tissue are not associated with risk for fetal orofacial clefts. Reprod. Toxicol. (IF 3.121) Pub Date : 2020-09-10 Xin Pi,Yiran Qiao,Chengrong Wang,Zhiwen Li,Jufen Liu,Linlin Wang,Lei Jin,Aiguo Ren
Previous epidemiological studies have shown that prenatal exposure to organochlorine pesticides (OCPs) entails a variety of adverse impacts on fetal health, but it is not yet known whether it is associated with risk for orofacial clefts (OFCs). This study of 103 fetuses or newborns with a diagnosis of OFCs (cases) and 103 healthy newborns without malformations (controls) examined whether prenatal exposure
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Comparative effects of TiO2 and ZnO nanoparticles on growth and ultrastructure of ovarian antral follicles. Reprod. Toxicol. (IF 3.121) Pub Date : 2020-08-13 Ramsés Santacruz-Márquez,Aidee Solorio-Rodríguez,Sirenia González-Posos,Sihomara Patricia García-Zepeda,Jaime Santoyo-Salazar,Andrea De Vizcaya-Ruiz,Isabel Hernández-Ochoa
Titanium dioxide (TiO2) and zinc oxide (ZnO) nanoparticles (NP) have been demonstrated to reach the ovary. However, the potential detrimental effects of these metal-based NP on ovarian antral follicles and whether they can be directly taken up by follicular cells are unknown. The aim of this study was to evaluate whether TiO2 and ZnO NP internalize into the antral follicle, and further compared any
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