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Anterior gradient 2 is involved in the post-transcriptional regulation of β-dystroglycan Anim. Cells Syst. (IF 0.907) Pub Date : 2021-01-17 Eunyoung Lee; Do Hee Lee
ABSTRACT Anterior gradient 2 (AGR2) is a protein disulfide isomerase over-expressed in numerous types of cancer. Although AGR2 plays a role in ER homeostasis, its function(s) in tumorigenesis is still elusive. Here we demonstrate that AGR2 is involved in the regulation of the β-subunit of dystroglycan (β-DG), a component of the multi-protein complex linking the extracellular matrix and cytoskeletal
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Microfluidic approaches for Caenorhabditis elegans research Anim. Cells Syst. (IF 0.907) Pub Date : 2020-11-03 Erel Levine; Kyung Suk Lee
ABSTRACT In the last decade, microfluidic methods have proven to be powerful tools for Caenorhabditis elegans research, offering advanced manipulation of worms and precise control of experimental conditions. The advantages of microfluidic chips include their capability of immobilization, automated sorting, and longitudinal measurement, and more. In this review, we focus on control components that are
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Risk prediction and marker selection in nonsynonymous single nucleotide polymorphisms using whole genome sequencing data Anim. Cells Syst. (IF 0.907) Pub Date : 2020-12-24 Young-Sup Lee; KyeongHye Won; Donghyun Shin; Jae-Don Oh
ABSTRACT Despite the various existing studies about nonsynonymous single nucleotide polymorphisms (nsSNPs), genome-wide studies based on nsSNPs are rare. NsSNPs alter amino acid sequences, affect protein structure and function, and have deleterious effects. By predicting the deleterious effect of nsSNPs, we determined the total risk score per individual. Additionally, the machine learning technique
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Terminal differentiation into adipocyte and growth inhibition by PPARγ activation in human A549 lung adenocarcinoma cells Anim. Cells Syst. (IF 0.907) Pub Date : 2020-12-02 Dae-Young Kim; Sun-Ha Moon; Jang-Ho Han; Mi-Jeong Kim; Seong-Ju Oh; Dinesh Bharti; Sung-Ho Lee; Jong-Kuen Park; Gyu-Jin Rho; Byeong-Gyun Jeon
ABSTRACT The present study investigated the terminal differentiation capacity into adipocytes and subsequent growth inhibition in A549 cancer cells treated with pioglitazone (PGZ), a PPARγ activator. The rate of cell growth in A549 cells was significantly (P < .05) inhibited in concentrations above 10 μM PGZ while maintaining less cytotoxic effects in MRC-5 fibroblasts. Following 50 μM PGZ treatment
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Inhibitors of synaptic vesicle exocytosis reduce surface expression of postsynaptic glutamate receptors Anim. Cells Syst. (IF 0.907) Pub Date : 2020-11-03 Dong Ho Woo; Young-Na Hur; Minwoo Wendy Jang; C. Justin Lee; Mikyoung Park
ABSTRACT Bafilomycin A1, a vacuolar H+-ATPase inhibitor, and botulinum toxin B and tetanus toxin, both vesicle fusion inhibitors, are widely known exocytosis blockers that have been used to inhibit the presynaptic release of neurotransmitters. However, protein trafficking mechanisms, such as the insertion of postsynaptic receptors and astrocytic glutamate-releasing channels into the plasma membrane
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Suppressive effects of valproic acid on caudal fin regeneration in adult zebrafish Anim. Cells Syst. (IF 0.907) Pub Date : 2020-12-24 Yunkyoung Lee; Dohee Kim; Chang-Joong Lee
ABSTRACT Zebrafish can regenerate fins following injury through an epimorphic process that includes the formation of new tissues and reconstruction of the original morphology. In this study, the effects of valproic acid (VPA), a widely used anti-epileptic drug, on fin regeneration were studied after the caudal fin amputation of adult zebrafish. In the control group, zebrafish formed new tissues and
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Dusp1 modulates activin/smad2 mediated germ layer specification via FGF signal inhibition in Xenopus embryos Anim. Cells Syst. (IF 0.907) Pub Date : 2020-11-27 Zobia Umair; Santosh Kumar; Khezina Rafiq; Vijay Kumar; Zia Ur Reman; Seung-Hwan Lee; SungChan Kim; Jae-Yong Lee; Unjoo Lee; Jaebong Kim
ABSTRACT Activin, a member of the transforming growth factor (TGF-β) superfamily, induces mesoderm, endoderm and neuro-ectoderm formation in Xenopus embryos. Despite several previous studies, the complicated gene regulatory network and genes involved in this induction await more elaboration. We identified expression of various fibroblast growth factor (FGF) genes in activin/smad2 treated animal cap
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CD47 (Cluster of differentiation 47): an anti-phagocytic receptor with a multitude of signaling functions Anim. Cells Syst. (IF 0.907) Pub Date : 2020-09-15 Pascal Leclair; Chinten James Lim
ABSTRACT CD47 is a tumor-associated antigen best known for its ability to bind counter-receptors on the surface of professional phagocytes as an immune-evasion strategy. Recently, CD47 has been shown to play a role as a signaling receptor, involving a number of cell physiological processes. This review provides a comprehensive survey of the signaling pathways triggered by CD47 ligand-mediated cell
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Aquaporin-1 facilitates proliferation and invasion of gastric cancer cells via GRB7-mediated ERK and Ras activation Anim. Cells Syst. (IF 0.907) Pub Date : 2020-10-21 Zhenjie Wang; Yujuan Wang; Yuan He; Ning Zhang; Wei Chang; Yahui Niu
ABSTRACT Gastric cancer, one of the most common malignant tumors of the digestive tract, is devoid of effective treatment owing to its highly invasive ability. Aquaporins (AQPs), transmembrane water channel proteins, has been shown to be involved in the malignancy of gastric cancer. This study aims to investigate the pathophysiological roles of AQP-1 in gastric cancer. We first demonstrated quantitative
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Arachidonic acid induces ER stress and apoptosis in HT-29 human colon cancer cells Anim. Cells Syst. (IF 0.907) Pub Date : 2020-09-01 Sijeong Bae; Min-Kyoung Kim; Hong Seok Kim; Young-Ah Moon
ABSTRACT Polyunsaturated fatty acids (PUFAs) have important functions in biological systems. The beneficial effects of dietary PUFAs against inflammatory diseases, cardiovascular diseases, and metabolic disorders have been shown. Studies using cancer cells have presented the anti-tumorigenic effects of docosahexaenoic acid (DHA), an n-3 PUFA, while arachidonic acid (AA), an n-6 PUFA, has been shown
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Upregulating miR-637 aggravates endoplasmic reticulum stress-induced apoptosis in gastric cancer cells by suppressing Calreticulin Anim. Cells Syst. (IF 0.907) Pub Date : 2020-09-10 Qingli Kong; Zhisheng Zhang; Zhipeng Liang
ABSTRACT Gastric cancer is a leading cause of cancer death worldwide. Endoplasmic reticulum (ER) stress-induced apoptosis has been confirmed to be important in the treatment of gastric cancer. MiR-637 has recently been found to exert inhibitory effects on gastric cancer, and this study aimed to investigate whether miR-637 could regulate apoptosis through ER stress. The results showed that tunicamycin
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miR-122 promotes diabetic retinopathy through targeting TIMP3 Anim. Cells Syst. (IF 0.907) Pub Date : 2020-09-10 Mingliang Wang; Huifen Zheng; Xianbo Zhou; Jiwei Zhang; Guanghui Shao
ABSTRACT Diabetic retinopathy (DR) is a primary complication of diabetes mellitus. DR can cause severe vision loss for patients. miR-122 is elevated in DR patients, while its role in DR is unclear. Hence, the purpose of this study was to analyze the effect of miR-122 on the function of high glucose-induced REC cells and the underlying molecular mechanisms. In this study, our results revealed that miR-122
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IFITM proteins inhibit the late step of feline foamy virus replication Anim. Cells Syst. (IF 0.907) Pub Date : 2020-09-15 Jinsun Kim; Cha-Gyun Shin
ABSTRACT Interferon-induced transmembrane (IFITM) proteins as host restriction factors are known to inhibit the replication of several viruses. In this study, transient IFITM expression vectors were used to investigate whether IFITMs inhibit feline foamy viral (FFV) replication and which step of viral replication is inhibited. In our studies, viral production was significantly reduced when cells were
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Induction of the differentiation of porcine bone marrow mesenchymal stem cells into premature hepatocyte-like cells in an indirect coculture system with primary hepatocytes Anim. Cells Syst. (IF 0.907) Pub Date : 2020-10-04 Imran Ullah; Kangmin Seo; Hayeon Wi; Youngim Kim; Seunghoon Lee; Sun A Ock
ABSTRACT Liver transplantation is currently the only option for patients with end-stage liver disease. Thus, other alternate therapeutic strategies are needed. Bone marrow mesenchymal stem cells (BM-MSCs) are nonhematopoietic cells present in the bone marrow stroma that serve as precursors cells for various other cells. In this study, we evaluated the differentiation of porcine BM-MSCs into hepatocyte-like
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Molt-related changes in the major ampullate silk gland of the barn spider Araneus cavaticus Anim. Cells Syst. (IF 0.907) Pub Date : 2020-10-29 Myung-Jin Moon; Edward K. Tillinghast
ABSTRACT Spiders molt periodically before reaching full maturity, but several spiders continue to molt after sexual maturity. This post-maturity molting (PMM) behavior has been observed in the barn spider Araneus cavaticus (Araneae: Araneidae) among the orb-web spiders. In this study, we investigated molt-related changes in the ampulla and tail regions of the major ampullate gland during the PMM sequences
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Various biological effects of solar radiation on skin and their mechanisms: implications for phototherapy Anim. Cells Syst. (IF 0.907) Pub Date : 2020-08-20 Dong Wook Shin
The skin protects our body from various external factors, such as chemical and physical stimuli, microorganisms, and sunlight. Sunlight is a representative environmental factor that considerably influences the physiological activity of our bodies. The molecular mechanisms and detrimental effects of ultraviolet rays (UVR) on skin have been thoroughly investigated. Chronic exposure to UVR generally causes
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Inhibition of transient receptor potential melastatin 7 (TRPM7) protects against Schwann cell trans-dedifferentiation and proliferation during Wallerian degeneration Anim. Cells Syst. (IF 0.907) Pub Date : 2020-08-05 Young Hwa Kim; Sumin Lee; Hyejin Yang; Yoo Lim Chun; Dokyoung Kim; Seung Geun Yeo; Chan Park; Junyang Jung; Youngbuhm Huh
Irreversible peripheral neurodegenerative diseases such as diabetic peripheral neuropathy are becoming increasingly common due to rising rates of diabetes mellitus; however, no effective therapeutic treatments have been developed. One of main causes of irreversible peripheral neurodegenerative diseases is dysfunction in Schwann cells, which are neuroglia unique to the peripheral nervous system (PNS)
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Plumbagin inhibits proliferation and promotes apoptosis of ovarian granulosa cells in polycystic ovary syndrome by inactivating PI3K/Akt/mTOR pathway Anim. Cells Syst. (IF 0.907) Pub Date : 2020-07-17 Zhaowei Cai; Shaojuan He; Tao Li; Li Zhao; Kerong Zhang
Polycystic ovary syndrome (PCOS) is recognized as a general endocrine disease and reproductive disorder. Although evidence indicates that PCOS has a complex etiology and genetic basis, the pathogenic mechanisms and signal pathway in PCOS remain unclear. In this study, the normal structure of follicle and corpus luteum were observed, and no cyst nor hyperemia was observed under the light microscopic
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Antidepressant effect and neural mechanism of Acer tegmentosum in repeated stress–induced ovariectomized female rats Anim. Cells Syst. (IF 0.907) Pub Date : 2020-08-18 Hyun-Jung Park; Hyun Soo Shim; SongYi Park; Insop Shim
Acer tegmentosum (ATM) has antioxidant and anti-adipogenic activity. However, few studies have investigated the pharmacological activity or mechanism of ATM as an antidepressant agent. We assessed the antidepressant effect of ATM in modulating menopausal depressive symptoms and its mechanisms in ovariectomized (OVX) and repeatedly stressed (RS) female rats. The female rats were randomly divided into
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Capsaicin suppresses liver fat accumulation in high-fat diet-induced NAFLD mice Anim. Cells Syst. (IF 0.907) Pub Date : 2020-08-26 Mi Kyung Shin; Soo-Man Yang; In-Seob Han
Dietary capsaicin exhibits anti-steatosis activity in obese mice. High-fat diet (HFD)-induced mice is a highly studied approach to develop non-alcoholic fatty liver disease (NAFLD). In this study, we determined whether the topical application of capsaicin can improve lesions of NAFLD. The HFD-induced mice were treated with daily topical application of capsaicin for 8 weeks. Topical application of capsaicin
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SPRY4-IT1 promotes survival of colorectal cancer cells through regulating PDK1-mediated glycolysis Anim. Cells Syst. (IF 0.907) Pub Date : 2020-06-25 Shengyuan Liu; Feng Huang; Qing Ye; Yangming Li; Jinhu Chen; Hong Huang
Colorectal cancer (CRC) becomes the third leading cause of cancer-related deaths worldwide recently. The prognosis of CRC is still poor in decades, and targeted therapy is still a potential effective treatment. Long non-coding RNAs (lncRNAs) could regulate series of cellular functions and developmental processes. LncRNA-SPRY4-IT1 (GenBank ID AK024556) is derived from an intron of the SPRY4 gene, which
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Localization of integrin heterodimer α9β1 on the surface of uterine endometrial stromal and epithelial cells in mice Anim. Cells Syst. (IF 0.907) Pub Date : 2020-08-05 Hye Jin Park; Jung Im Yun; Seung Tae Lee
Previously, we reported that endometrial stromal (ES) and endometrial epithelial (EE) cells did not attach to tenascin C, indicating the absence of active integrin α9β1 on the surface of mouse ES and EE cells. However, that study used recombinant tenascin C without fibronectin (FN) type III repeats interacting with integrin heterodimers. Therefore, we re-evaluated the presence of integrin α9β1 actively
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The immunostimulatory effect of indole-6-carboxaldehyde isolated from Sargassum thunbergii (Mertens) Kuntze in RAW 264.7 macrophages Anim. Cells Syst. (IF 0.907) Pub Date : 2020-08-20 Cheol Park; Hyun HwangBo; Hyesook Lee; Gi-Young Kim; Hee-Jae Cha; Sung Hyun Choi; Suhkmann Kim; Heui-Soo Kim; Seok Joong Yun; Wun-Jae Kim; You-Jin Jeon; Yung Hyun Choi
Indole-6-carboxaldehyde (I6CA), an indole derivative isolated from the marine brown algae Sargassum thunbergii, is known to have several beneficial effects, but no studies on immune regulation have been conducted. In this study, the immunomodulatory properties of I6CA on murine RAW 264.7 monocyte/macrophage cells were evaluated. As the concentration of I6CA increased, the morphology of RAW 264.7 cells
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Granulosa cell proliferation is inhibited by PGE2 in the primate ovulatory follicle Anim. Cells Syst. (IF 0.907) Pub Date : 2020-05-21 Patric S. Lundberg; Gil J. Moskowitz; Carmel Bellacose; Esra Demirel; Heidi A. Trau; Diane M. Duffy
ABSTRACT Prostaglandin E2 (PGE2) is a key paracrine mediator of ovulation. Few specific PGE2-regulated gene products have been identified, so we hypothesized that PGE2 may regulate the expression and/or activity of a network of proteins to promote ovulation. To test this concept, Ingenuity Pathway Analysis (IPA) was used to predict PGE2-regulated functionalities in the primate ovulatory follicle. Cynomolgus
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miR-424-5p regulates apoptosis and cell proliferation via targeting Bcl2 in nucleus pulposus cells Anim. Cells Syst. (IF 0.907) Pub Date : 2020-06-23 Hua-tuo Lu; Yong-qing Xu; Hai Wang; Xu-lin Zhang
ABSTRACT Background miRNAs play an important role in the pathogenesis of intervertebral disc degeneration (IDD). The role and the underlying mechanism of miR-424-5p in human nucleus pulposus (NP) are still unknown. We aimed to explore the role of miR-424-5p in IDD. Methods Real-time PCR was used to detect the expression of miR-424-5p and Bcl2 in IDD tissues and idiopathic scoliosis tissues. Human NP
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Antinociceptive effect of chrysin in diabetic neuropathy and formalin-induced pain models Anim. Cells Syst. (IF 0.907) Pub Date : 2020-05-25 Jae-Seung Hong; Jing-Hui Feng; Jung-Seok Park; Hee-Jung Lee; Jae-Yong Lee; Soon-Sung Lim; Hong-Won Suh
ABSTRACT Chrysin, a natural flavonoid, is the main ingredient of many medicinal plants, which shows potent pharmacological properties. In the present study, the antinociceptive effects of chrysin were examined in ICR mice. Chrysin orally administered at the doses of from 10 to 100 mg/kg exerted the reductions of formalin-induced pain behaviors observed during the second phase in the formalin test in
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Treatment of growth hormone attenuates hepatic steatosis in hyperlipidemic mice via downregulation of hepatic CD36 expression Anim. Cells Syst. (IF 0.907) Pub Date : 2020-06-12 Hyung Seok Jang; Kyeongdae Kim; Mi-Ran Lee; Shin-Hye Kim; Jae-Hoon Choi; Mi Jung Park
ABSTRACT The recombinant human growth hormone (GH) has been used for the treatment of growth hormone deficiency (GHD) and diverse short stature state, and its physiological and therapeutic effects are well documented. However, since the effect of GH treatment on metabolic disorders has not been well characterized, we injected GH to Western diet-fed low-density lipoprotein receptor-deficient (Ldlr −/−)
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Construction and validation of a seven-gene signature for predicting overall survival in patients with kidney renal clear cell carcinoma via an integrated bioinformatics analysis Anim. Cells Syst. (IF 0.907) Pub Date : 2020-05-12 Huiming Jiang; Haibin Chen; Nanhui Chen
ABSTRACT Kidney renal clear cell carcinoma (KIRC) remains a significant challenge worldwide because of its poor prognosis and high mortality rate, and accurate prognostic gene signatures are urgently required for individual therapy. This study aimed to construct and validate a seven-gene signature for predicting overall survival (OS) in patients with KIRC. The mRNA expression profile and clinical data
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Genetic integrity and individual identification-based population size estimate of the endangered long-tailed goral, Naemorhedus caudatus from Seoraksan National Park in South Korea, based on a non-invasive genetic approach Anim. Cells Syst. (IF 0.907) Pub Date : 2020-06-25 Ji Eun Jang; Nam Hyeong Kim; Sangjin Lim; Ki Yoon Kim; Hyuk Je Lee; Yung Chul Park
ABSTRACT The long-tailed goral (also called the Amur goral) Naemorhedus caudatus (subfamily Caprinae), a vulnerable and protected species designated by IUCN and CITES, has sharply been declining in the population size and is now becoming critically endangered in South Korea. This species has been conserved as a natural monument by the Korean Cultural Heritage Administration since 1968. In this study
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JAK2-STAT5 signaling is insensitive to porcine growth hormone (pGH) in hepatocytes of neonatal pig. Anim. Cells Syst. (IF 0.907) Pub Date : 2020-04-27 Yang Yu-Jiang,Zheng Xin,Lan Hai-Nan
ABSTRACT Porcine growth hormone (pGH) is most important hormone which is involved in the growth and development of pig. However, a series of studies have indicated that neonatal pig is insensitive to pGH; the reason for this phenomenon is still not fully understood. In this work, we try to investigate this issue from the angle of intracellular signaling induced by pGH. In the present study, porcine
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Trim45 is essential to the development of the diencephalon and eye in zebrafish embryos. Anim. Cells Syst. (IF 0.907) Pub Date : 2020-04-22 Seoyeon Choe,Tae-Lin Huh,Myungchull Rhee
ABSTRACT Trim45 is one of the RING (really interesting new gene) finger containing E3 ligase, which belongs to TRIM (Tripartite motif) protein family. Its molecular biological functions have been well characterized but not in light of developmental aspects. Here, we are reporting its expression patterns and developmental functions in zebrafish embryos. First, maternal transcripts of trim45 were found
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Generation of embryonic stem cells derived from the inner cell mass of blastocysts of outbred ICR mice. Anim. Cells Syst. (IF 0.907) Pub Date : 2020-04-16 Na Rae Han,Song Baek,Hwa-Young Kim,Kwon Young Lee,Jung Im Yun,Jung Hoon Choi,Eunsong Lee,Choon-Keun Park,Seung Tae Lee
ABSTRACT Embryonic stem cells (ESCs) derived from outbred mice which share several genetic characteristics similar to humans have been requested for developing stem cell-based bioengineering techniques directly applicable to humans. Here, we report the generation of ESCs derived from the inner cell mass of blastocysts retrieved from 9-week-old female outbred ICR mice mated with 9-week-old male outbred
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Avenanthramide C suppresses hypoxia-induced cyclooxygenase-2 expression through sirtuin1 activation in non-small-cell lung cancer cells. Anim. Cells Syst. (IF 0.907) Pub Date : 2020-04-02 Wonchung Lim,Chounghun Kang
Summary Avenanthramide C (AVC), found mainly in oats, mediates anti-inflammatory activities by reducing the anti-inflammatory cytokine levels. This study investigated the effects of AVC on hypoxia-induced cyclooxygenase-2 (COX-2) expression in A549 cells. AVC suppressed the hypoxia-induced increase in COX-2 protein levels and promoter activity. We also observed that the effects of AVC were reversed
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Hesperidin depolarizes the pacemaker potentials through 5-HT4 receptor in murine small intestinal interstitial cells of Cajal. Anim. Cells Syst. (IF 0.907) Pub Date : 2020-03-26 Minwoo Hwang,Jeong Nam Kim,Byung Joo Kim
ABSTRACT Hesperidin, a citrus flavonoid, can exert numerous beneficial effects on human health. Interstitial cells of Cajal (ICC) are pacemaker cells in the gastrointestinal (GI) tract. In the present study, we investigated potential effects of hesperidin on pacemaker potential of ICC in murine small intestine and GI motility. A whole-cell patch-clamp configuration was used to record pacemaker potential
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Identification and prevention of heterotopias in mouse neocortical neural cell migration incurred by surgical damages during utero electroporation procedures. Anim. Cells Syst. (IF 0.907) Pub Date : 2020-03-09 Bolin Wang,Liting Ji,Kausik Bishayee,Changyu Li,Sung-Oh Huh
ABSTRACT In utero electroporation (IUE) is a useful technique for gene delivery in embryonic mouse brain. IUE technique is used to investigate the mammalian brain development in vivo. However, according to recent studies, IUE methodology has some limitations like the formation of artificial ectopias and heterotopias at the micro-injection site. Thus far, the artificial heterotopias generated by physical
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Knockdown of LncRNAZFAS1 suppresses cell proliferation and metastasis in non-small cell lung cancer. Anim. Cells Syst. (IF 0.907) Pub Date : 2020-03-02 Yong Zhou,Xiao-Wei Hu,Si-Jia Yang,Zhe Yu
ABSTRACT To evaluate the effects of LncRNAZFAS1 on cell proliferation and tumor metastasis in non-small cell lung cancer (NSCLC), we detected the expression level of LncRNAZFAS1 in NSCLC-related tissues and cells. qRT-PCR results revealed that LncRNAZFAS1 in tumor tissues was significantly higher than that in normal lung tissue, especially significantly up-regulated in stage III / IV and in metastatic
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Palmitate-induced autophagy liberates monounsaturated fatty acids and increases Agrp expression in hypothalamic cells Anim. Cells Syst. (IF 0.907) Pub Date : 2019-12-01 Seokjae Park, Tae Seok Oh, Seolsong Kim, Eun-Kyoung Kim
Fatty acids regulate food intake, although the exact mechanism remains unknown. Emerging evidence suggests that intracellular free fatty acids generated by starvation-induced autophagy regulate food intake. Starvation for 6 h elevated fatty acids such as palmitate, oleate, arachidonate, eicosatrienoate, and docosahexaenoate in the mouse serum. Among them, palmitate induced lipophagy, an autophagic
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Differential cell-type-expression of CYFIP1 and CYFIP2 in the adult mouse hippocampus Anim. Cells Syst. (IF 0.907) Pub Date : 2019-11-24 Yinhua Zhang, Hyae Rim Kang, Kihoon Han
Recent molecular genetic studies have suggested that two members of the cytoplasmic FMR1-interacting protein (CYFIP) gene family, CYFIP1 and CYFIP2, are causally associated with several brain disorders. However, the clinical features of individuals with CYFIP1 and CYFIP2 variants are quite different. In addition, null mice for either Cyfip1 or Cyfip2 are lethal, indicating that these two genes cannot
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AEG-1 aggravates inflammation via promoting NALP3 inflammasome formation in murine endometriosis lesions Anim. Cells Syst. (IF 0.907) Pub Date : 2019-11-21 Juan Zhao, Wei Ma, Weizhi Chen, Jie Gao, Chunling Li, Yahong Tong, Qin Zhou, Xiuling Zhao, Menghua Wang, Huan Xiao, Yanrong Jin
Endometriosis (EMs) is one of the most common gynaecological diseases in women of childbearing age. Astrocyte elevated gene-1 (AEG-1) is associated with the invasion, migration, apoptosis and prognosis of various cancers. However, the roles of AEG-1 in EMs and its corresponding molecular mechanism are still unknown. In this study, animal models of EMs were established and mice were divided into two
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Calcineurin tax-6 regulates male ray development and counteracts with kin-29 kinase in Caenorhabditis elegans Anim. Cells Syst. (IF 0.907) Pub Date : 2019-11-16 Seung Hyun Kim, Hana Jung, Joohong Ahnn, Sun-Kyung Lee
Phosphorylation is one of the critical protein modifications, which can lead to changing the activity of the proteins and regulating a variety of biological processes. Therefore, it is essential to properly maintain the phosphorylation level on proteins by balancing the activity of kinases and phosphatases. In this study, we report that calcineurin, a serine/threonine phosphatase, counteracts with
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Dexmedetomidine ameliorates memory impairment in sleep-deprived mice Anim. Cells Syst. (IF 0.907) Pub Date : 2019-11-16 Lakkyong Hwang, Il-Gyu Ko, Jun-Jang Jin, Sang-Hoon Kim, Chang-Ju Kim, Boksoon Chang, Jeong Ho Rho, Eun-Jin Moon, Jae-Woo Yi
The selective α2-adrenergic receptor agonist dexmedetomidine acts as an analgesic, sedative, and anesthetic adjuvant. The most common consequence of sleep deprivation is memory impairment. We investigated whether dexmedetomidine can counteract memory impairment caused by sleep deprivation and suppress the production of inflammatory factors. For inducing sleep deprivation, adult male mice were placed
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Predicting climate-driven shifts in the breeding phenology of Varied Tits (Sittiparus various) in South Korean forests Anim. Cells Syst. (IF 0.907) Pub Date : 2019-10-10 Min-Su Jeong, Chang-Young Choi, Hankyu Kim, Woo-Shin Lee
Phenological shifts of plants and animals due to climate change can vary among regions and species, requiring study of local ecosystems to understand specific impacts. The reproductive timing of insectivorous songbirds in temperate forests is tightly synchronized with peak prey abundance, and thus they can be susceptible to such shift in timing. We aimed to investigate the effect of future climate
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Reprogramming of spermatogonial stem cells into pluripotent stem cells in the spheroidal state Anim. Cells Syst. (IF 0.907) Pub Date : 2019-10-08 Yukyeong Lee, Minseong Lee, Seung-Won Lee, Na Yong Choi, Seokbeom Ham, Hye Jeong Lee, Kisung Ko, Kinarm Ko
Spermatogonial stem cells (SSCs) are unipotent adult stem cells, capable of differentiating into sperm cells. SSCs can be cultured in vitro for a long time. SSCs expressing Oct4, a pluripotency marker, and are the only adult cells which pluripotency can be induced under defined culture conditions. However, because 2D culture imposes limitations in cell junction formation, cell shape, metabolism, response
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Shwachman-Bodian-Diamond syndrome protein desensitizes breast cancer cells to apoptosis in stiff matrices by repressing the caspase 8-mediated pathway Anim. Cells Syst. (IF 0.907) Pub Date : 2019-09-20 Jieun Lee, Panseon Ko, Eunae You, Jangho Jeong, Seula Keum, Jaegu Kim, Mizanur Rahman, Dong Ho Lee, Sangmyung Rhee
Certain cancer types, including breast cancer, are accompanied with stiffening of the surrounding extracellular matrix (ECM). Previous studies suggest that this stiffened matrix influences cancer cell progression, such as proliferation and invasion, both biochemically and mechanically. However, the contribution of ECM stiffness to cellular response to diverse stresses, which most cancer cells are exposed
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Ubiquitin-specific peptidase 3 induces TPA-mediated leukemia cell differentiation via regulating H2AK119ub. Anim. Cells Syst. (IF 0.907) Pub Date : 2019-09-06 Yun-Cheol Chae,Hyeonsoo Jung,Ji-Young Kim,Dong Ho Lee,Sang-Beom Seo
Acute myeloid leukemia (AML) is the most common type of leukemia in adults. Owing to the chemotherapy associated side effects and toxicity, it is necessary to find a new mechanism, which can identify new potential therapeutic targets at the molecular level. Here, we identified new target genes that are induced during the TPA-induced HL-60 cell differentiation by ChIP-seq and microarray data analysis
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Effects of eicosapentaenoic acid and docosahexaenoic acid on C2C12 cell adipogenesis and inhibition of myotube formation. Anim. Cells Syst. (IF 0.907) Pub Date : 2019-09-03 Saeed Ghnaimawi,Sarah Shelby,Jamie Baum,Yan Huang
Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) modulate cellular metabolic functions and gene expression. This study investigated the impacts of EPA and DHA on gene expression and morphological changes during adipogenic inducement in C2C12 myoblasts. Cells were cultured and treated with differentiation medium with and without 50 μM EPA and DHA. Cells treated with fatty acids had noticeable
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HIF1α promotes prostate cancer progression by increasing ATG5 expression. Anim. Cells Syst. (IF 0.907) Pub Date : 2019-08-28 Kaiyuan Yu,Luxia Xiang,Shaoxun Li,Shuaibin Wang,Chaohao Chen,Haiqi Mu
Prostate cancer (PCa) is the most frequently diagnosed cancer among men. However, the major modifiable risk factors for PCa are poorly known and its specific mechanism of progression remains unclear. Here we reported that, in prostate cancer cells, the autophagy level was elevated under hypoxic condition, as well as the mRNA and protein level of ATG5, which is an important gene related to autophagy
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Prognostic role of the beta-2 adrenergic receptor in clear cell renal cell carcinoma. Anim. Cells Syst. (IF 0.907) Pub Date : 2019-08-25 Mihyang Ha,Dong Woo Kim,Jayoung Kim,Chae Mi Hong,Su Min Park,In Ae Woo,Min Yong Kim,Hyunjun Koo,Jin Namkoong,Jaehyun Kim,Myoung-Eun Han,Parkyong Song,Jin Hur,Chi-Dug Kang,Yun Hak Kim,Dongjun Lee,Sae-Ock Oh
The beta-2 adrenergic receptor (ADRB2) regulates the proliferation, apoptosis, angiogenesis, migration, and metastasis of cancer cells. However, its function in the progression of clear cell renal cell carcinoma (ccRCC) is unknown. Here, we report that ADRB2 can be a novel prognostic factor for patients with ccRCC. The differential expression of ADRB2 in low-stage (stages I and II), high-stage (stages
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Vascular endothelial growth factor improves the therapeutic effects of cyclodextrin in Niemann-Pick type C mice. Anim. Cells Syst. (IF 0.907) Pub Date : 2019-08-25 Min Seock Jeong,Jae-Sung Bae,Hee Kyung Jin
Niemann-Pick type C disease (NP-C) is a fatal neurodegenerative disorder caused by a deficiency in the function of the NPC1 gene. Malfunction of this gene/protein leads to progressive accumulation of unesterified cholesterol and sphingolipids in many organs, including the brain. To date, drugs that target pivotal stages in the pathogenic cascade have been tested as monotherapies or in combination with
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Endogenous leptin promotes autophagy in EBSS-induced PFCs. Anim. Cells Syst. (IF 0.907) Pub Date : 2019-08-16 Deling Jiao,Zhen Yang,Lulu Wang,Binyue Hu,Jing Wang,Anyong Xu,Wenmin Cheng,Baoyu Jia,Yubo Qing,Hong-Ye Zhao,Hong-Jiang Wei
Leptin is an important adipokine and plays a vital role in animals. However, the role of leptin in the autophagic response of pig fibroblast cells (PFCs) has not been fully elucidated. In this study, we investigated the relationship between leptin and autophagy as well as underlying molecular basis. We found that PFCs treated with EBSS could secrete leptin, and the leptin concentration in the supernatant
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Delay of cell growth and loss of stemness by inhibition of reverse transcription in human mesenchymal stem cells derived from dental tissue. Anim. Cells Syst. (IF 0.907) Pub Date : 2019-08-13 Won-Cheol Lee,Dae-Young Kim,Mi-Jeong Kim,Hyeon-Jeong Lee,Dinesh Bharti,Sung-Ho Lee,Young-Hoon Kang,Gyu-Jin Rho,Byeong-Gyun Jeon
The present study investigated the cellular properties in the dental tissue-derived mesenchymal stem cells (DSCs) exposed to nevirapine (NVP), an inhibitor of reverse transcriptase (RTase). After a prolonged exposure of DSCs for 2 weeks, the population doubling time (PDT) was significantly (P < .05) increased by delayed cell growth in the DSCs treated with 250 and 500 μM NVP, compared with untreated
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KAT5 Negatively regulates the proliferation of prostate cancer LNCaP cells via the caspase 3-dependent apoptosis pathway. Anim. Cells Syst. (IF 0.907) Pub Date : 2019-08-01 Chul-Hong Kim,Dong Ho Lee
Prostate cancer is one of the most common cancers in men over the age of sixty. Lysine acetyltransferase 5 (KAT5) is a histone acetyltransferase involved in transcriptional regulation, DNA repair, and cell signaling pathways. Previous studies have shown that KAT5 expression is reduced in the cytoplasm of the prostate cancer cell line LNCaP when exposed to androgen. Moreover, KAT5 has been reported
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Long-term exposure to moderate noise induces neural plasticity in the infant rat primary auditory cortex. Anim. Cells Syst. (IF 0.907) Pub Date : 2019-07-19 Chenchen Xia,Manli Yin,Ping Pan,Fanghao Fang,You Zhou,Yonghua Ji
Previous studies have reported that rearing infant rat pups in continuous moderate-level noise delayed the formation of topographic representational order and the refinement of response selectivity in the primary auditory (A1) cortex. The present study further verified that exposure to long-term moderate-intensity white noise (70 dB sound pressure level) from postnatal day (P) 12 to P30 elevated the
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Comparative analysis of three different protocols for cholinergic neuron differentiation in vitro using mesenchymal stem cells from human dental pulp. Anim. Cells Syst. (IF 0.907) Pub Date : 2019-06-10 Young-Hoon Kang,Sharath Belame Shivakumar,Young-Bum Son,Dinesh Bharti,Si-Jung Jang,Kang-Sun Heo,Won-Uk Park,June-Ho Byun,Bong-Wook Park,Gyu-Jin Rho
A decrease in the activity of choline acetyltransferase, the enzyme responsible for acetylcholine synthesis in the cholinergic neurons cause neurological disorders involving a decline in cognitive abilities, such as Alzheimer’s disease. Mesenchymal stem cells (MSCs) can be used as an efficient therapeutic agents due to their neuronal differentiation potential. Different source derived MSCs may have
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Endothelin-converting enzyme-1 expression in acute and chronic liver injury in fibrogenesis. Anim. Cells Syst. (IF 0.907) Pub Date : 2019-06-09 Tae-Jun Cho,Hyo-Jung Kim,Jaejin Cho
Endothelin-1 (ET-1) induces contraction, proliferation, and collagen synthesis of activated hepatic stellate cells and is a potent mediator of portal hypertension. Endothelin-converting enzyme-1 (ECE-1) generates ET-1 from the inactive precursor big-endothelin-1. The cellular distribution and activity of ECE-1 in the liver is unknown. Hepatic fibrogenesis was induced in rats by CCl4 administration
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Effects of resveratrol and oxyresveratrol on hippocampal cell death induced by kainic acid. Anim. Cells Syst. (IF 0.907) Pub Date : 2019-05-31 Hee-Jung Lee,Jing-Hui Feng,Su-Min Sim,Soon-Sung Lim,Jae-Yong Lee,Hong-Won Suh
In the present study, we have examined the possible neuroprotective effects of resveratrol and oxyresveratrol against kainic-acid (KA)-induced hippocampal neuronal cell death. Either resveratrol or oxyresvertrol was orally administered 30 min prior to intracerebroventricular (i.c.v.) administration with KA (0.05 μg). Oral pretreatment with oxyresveratrol (50 mg/kg) significantly protected KA-induced
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Paternal bias expression of Igf2as is enhancer-dependent on the imprinting cluster of Igf2, H19 and Nctc1 in muscle cells. Anim. Cells Syst. (IF 0.907) Pub Date : 2019-05-16 Jae-Young Kim,Hwa Young Youn,Karl Pfeifer,Bokkee Eun
Igf2, H19, and Nctc1 are linked co-regulated genes on distal mouse chromosome 7. This locus is an important model both for studying mechanisms of monoallelic expression and for elucidating the role of cis-regulatory elements – enhancers and insulators – in organizing chromatin and gene expression across a large domain. In this study we characterize regulated expression of the Igf2 antisense transcript
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Pharmacokinetic, metabolic stability, plasma protein binding and CYP450s inhibition/induction assessment studies of N-(2-pyridylmethyl)-2-hydroxiymethyl-1-pyrrolidinyl-4-(3-chloro-4-methoxy-benzylamino)-5-pyrimidine-carboxamide as potential type 5 phosphodiesterase inhibitors. Anim. Cells Syst. (IF 0.907) Pub Date : 2019-05-16 Haijun Qu,Xiaoxiao Hu,Xiaoli Shi,Chuan Wang,Longyuan Wang,Guoping Wang
N-(2-pyridylmethyl)-2-hydroxiymethyl-1-pyrrolidinyl-4-(3-chloro-4-methoxy-benzylamino)-5-pyrimidine-carboxamide (NHPPC) is a new potential of type 5 phosphodiesterase (PDE5) inhibitors, synthesized from the avanafil analogue for the treatment of erectile dysfunction. The targets of this article were to assess plasma protein binding, liver microsomal metabolic stability, inhibition and induction on
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Metformin inhibits cervical cancer cell proliferation via decreased AMPK O-GlcNAcylation. Anim. Cells Syst. (IF 0.907) Pub Date : 2019-05-14 Min Young Kim,Yoon Sook Kim,Minjun Kim,Mee Young Choi,Gu Seob Roh,Dong Hoon Lee,Hyun Joon Kim,Sang Soo Kang,Gyeong Jae Cho,Jeong Kyu Shin,Wan Sung Choi
Metformin is a widely used drug for the treatment of type 2 diabetes. Antidiabetic drugs are also known to influence cancer progression, as high glucose levels affect both cancer and diabetes. Metformin induces cell cycle arrest in cancer cells, but the underlying mechanism remains unclear in cervical cancer system. Here, we examined how metformin affects cell cycle arrest and apoptosis in cervical
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Anoctamin-1 affects the migration and invasion of anaplastic thyroid carcinoma cells. Anim. Cells Syst. (IF 0.907) Pub Date : 2019-05-10 Jae-Young Kim,Hwa Young Youn,June Choi,Seung Kuk Baek,Soon Young Kwon,Bok Kee Eun,Jae-Yong Park,Kyoung Ho Oh
Anaplastic thyroid carcinoma (ATC) is a rare malignancy with very poor prognosis. The exact cause underlying its strong aggressive nature is not clear. Here, we discovered t