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Predicting potential residues associated with lung cancer using deep neural network Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-12-11 Medha Pandey; M. Michael Gromiha
Lung cancer is a prominent type of cancer, which leads to high mortality rate worldwide. The major lung cancers lung adenocarcinoma (LUAD) and lung squamous carcinoma (LUSC) occur mainly due to somatic driver mutations in proteins and screening of such mutations is often cost and time intensive. Hence, in the present study, we systematically analyzed the preferred residues, residues pairs and motifs
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The FHA domain of PNKP is essential for its recruitment to DNA damage sites and maintenance of genome stability Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-11-02 Kaima Tsukada; Mikio Shimada; Rikiya Imamura; Kotaro Saikawa; Masamichi Ishiai; Yoshihisa Matsumoto
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Targeting DNA damage response kinases in cancer therapy Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-11-01 Vanesa Gottifredi
Cancer cells die when their decimated DNA damage response (DDR) unsuccessfully handles DNA damage. This notion has been successfully exploited when targeting PARP (poly ADP-ribose polymerase) in homologous recombination-deficient cells. With the greater understanding of DDR achieved in the last decade, new cancer therapy targets within the DDR network have been identified. Intriguingly, many of the
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The long-term effects of exposure to ionising radiation on gene expression in mice Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-10-11 Ayman Jafer; Nicolas Sylvius; Adeolu B. Adewoye; Yuri E. Dubrova
Despite great advancement in our understanding of the biological response to ionising radiation in mammals, a number of pertinent questions remain unanswered. For instance, the mechanisms underlying the long-term effects of acute radiation in vivo still eludes us. Here we report that acute exposure to X-rays in male mice significantly affects their transcriptome. Using microarrays and miRNA-sequencing
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Distinct epigenetic regulation in patients with multidrug-resistant TB-HIV co-infection and uninfected individuals Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-10-13 Musa Marimani; Suliman Yousef AlOmar; Badr aldahmash; Aijaz Ahmad; Sarah Stacey; Adriano Duse
Background: Mycobacterium tuberculosis (Mtb) is an airborne pathogenic microorganism that causes tuberculosis (TB). This pathogen invades lung tissues causing pulmonary infections and disseminates into other host organs. The Bacillus Calmette-Guérin (BCG) vaccine is employed to provide immune protection against TB; however, its efficacy is dependent on the age, immune status and geographic location
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A novel pathway for the induction of DNA damage in human spermatozoa involving extracellular cell-free DNA. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-09-01 Robert John Aitken,Sara Whiting,Haley Connaughton,Ben Curry,Torsten Reinheimer,Marcel van Duin
DNA damage is a common feature of human spermatozoa associated with an impaired capacity to fertilize the oocyte and an increased mutational load in the offspring. However, the etiology of this damage remains poorly defined. In this study we demonstrate that a major pathway for the induction of DNA damage in mammalian spermatozoa is triggered by exposure to exogenous cell free DNA (cfDNA). Exposure
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Synonymous codon usage and context analysis of genes associated with pancreatic cancer. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-08-11 Supriyo Chakraborty,Sunanda Paul,Durbba Nath,Yashmin Choudhury,Yeongseon Ahn,Yoon Shin Cho,Arif Uddin
Pancreatic cancer is a fatal disorder which originates in pancreas. Its mortality rate is increasing with time. Some studies also reported that pancreatic cancer would be ranked 2nd by the year 2030. Codon usage bias (CUB) arises when synonymous codons for each amino acid are not used randomly in the coding sequences of genes. We used bioinformatic methods to analyze the compositional properties, codon
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Induction of proliferative and mutagenic activity by benzo(a)pyrene in PC-3 cells via JAK2/STAT3 pathway. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-08-12 Meili Gao,Hong Li,Fan Dang,Lan Chen,Xiaojing Liu,Jianghong Gao
Environmental carcinogen benzo(a)pyrene (BaP) is a representative compound of polycyclic aromatic hydrocarbons (PAHs). BaP is strongly associated with prostate carcinogenesis. However, the molecular mechanism of BaP in development of prostate carcinoma remains largely unknown. The aim of this study was to investigate the effect and mechanism of BaP on the development in prostate cancer. PC-3 cells
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Mutational screening of PKD1 and PKD2 in Indian ADPKD patients identified 95 genetic variants. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-08-11 Sonam Raj,Rana Gopal Singh,Parimal Das
Background Mutation screening of autosomal dominant polycystic kidney disease (ADPKD) cases imply the major involvement of PKD1 mutations in 85% of patients while rest of the cases harbor mutation in PKD2, DNAJB11 and GANAB. This essentially indicates that individual’s genotype holds the key for disease susceptibility and its severity. Methods For finding genetic variability underlying the disease
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Nuclear mechanosensing: mechanism and consequences of a nuclear rupture. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-08-05 Gengqiang Xie,Reddick R Walker,Jerome Irianto
The physical connections between the cytoskeletal system and the nucleus provide a route for the nucleus to sense the mechanical stress both inside and outside of the cell. Failure to withstand such stress leads to nuclear rupture, which is observed in human diseases. In this review, we will go through the recent findings and our current understandings of nuclear rupture. Starting with the triggers
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Aurora kinases and DNA damage response. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-07-23 Hoi Tang Ma,Randy Y C Poon
It is well established that Aurora kinases perform critical functions during mitosis. It has become increasingly clear that the Aurora kinases also perform a myriad of non-mitotic functions including DNA damage response. The available evidence indicates that inhibition Aurora kinase A (AURKA) may contribute to the G2 DNA damage checkpoint through AURKA’s functions in PLK1 and CDC25B activation. Both
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Nuclear actin: The new normal. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-07-21 Leonid Serebryannyy,Primal de Lanerolle
The presence of actin in the nucleus has historically been a highly contentious issue. It is now, however, well accepted that actin has physiologically important roles in the nucleus. In this Review, we describe the evolution of our thinking about actin in the nucleus starting with evidence supporting its involvement in transcription, chromatin remodeling and intranuclear movements. We also review
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Moving fast and breaking things: Incidence and repair of DNA damage within ribosomal DNA repeats. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-07-17 Yana P Blokhina,Abigail Buchwalter
The genes that code for ribosomal RNA are present in hundreds of tandemly arrayed copies in the human genome. Ribosomal DNA repeats transcribe vast amounts of ribosomal RNA in order to meet the cell’s relentless demand for ribosome production. Intrinsic features of ribosomal DNA repeats render them uniquely vulnerable to DNA damage. Sensing and repairing damage to ribosomal DNA involves dramatic spatial
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Virtual screening of mutations in antioxidant genes and its putative association with HNSCC: An in silico approach. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-06-09 Vijayashree Priyadharsini J,Paramasivam A
Abnormalities in the antioxidant pathway are usually associated with inflammatory conditions, followed by tissue damage. Cancer is one such disease where there is a build-up of reactive oxygen species leading to pathological consequences. The present study aims to identify the alteration in genes and proteins associated with the common antioxidant pathways among patients with head and neck squamous
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Toxic effect and genotoxicity of carvacrol ethers in Drosophila melanogaster. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-06-18 Mariia Nesterkina,Svitlana Bilokon,Tetiana Alieksieieva,Sabina Chebotar,Iryna Kravchenko
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Chromatin rigidity provides mechanical and genome protection. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-06-17 Andrew D Stephens
The nucleus is the organelle in the cell that contains the genome and its associate proteins which is collectively called chromatin. New work has shown that chromatin and its compaction level, dictated largely through histone modification state, provides rigidity to protect and stabilize the nucleus. Alterations in chromatin, its mechanics, and downstream loss of nuclear shape and stability are hallmarks
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Blood and saliva-derived exomes from healthy Caucasian subjects do not display overt evidence of somatic mosaicism. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-05-18 Nathan E Hall,Jared Mamrot,Chris Frampton,Prue Read,Edward J Steele,Robert J Bischof,Robyn A Lindley
Somatic mosaicism is a normal occurrence during development in the tissues and organs. As part of establishing a “healthy population “(HP) background or base-line, we investigated whether such mosaicism can be routinely detected in the circulating DNA secured from a rigorously designed healthy human liquid biopsy clinical trial (saliva, blood). We deployed next generation (NG) whole exome sequencing
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Haplotype analysis of the CFTR gene on normal and mutant CFTR genes. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-05-23 Nasibeh Karimi,Ali Bidemeshki Pour,Reza Alibakhshi,Shekoufeh Almasi
Background Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene are responsible for Cystic Fibrosis (CF) disease. Since the distribution of polymorphisms varies among populations, a comparison between the frequency of CFTR polymorphisms in patients and healthy population may further identify their role in CF disease. The results obtained from this research may facilitate
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New insight into the biology of R-loops. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-05-28 Prasun Chakraborty
R-loops form when RNA hybridizes with its template DNA generating a three-stranded structure leaving a displaced single strand non-template DNA. During transcription negative supercoiling of DNA behind the advancing RNA polymerase will facilitate the formation of R-loops by the nascent RNA as the DNA is under wound to facilitate transcription. In theory R-loops are classified into pathological and
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Monitoring global chromatin dynamics in response to DNA damage. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-05-26 Haitham A Shaban,Andrew Seeber
DNA damage induced global chromatin motion has been observed in yeast and mammalian cells. Currently, it is unclear what mechanisms may be driving these changes in whole genome dynamics. Recent advances in live-cell microscopy now enable chromatin motion to be quantified throughout the whole nucleus. In addition, much work has improved quantification of single particle trajectories. This topic is particularly
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Polymer perspective of genome mobilization. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-05-26 Colleen J Lawrimore,Josh Lawrimore,Yunyan He,Sergio Chavez,Kerry Bloom
Chromosome motion is an intrinsic feature of all DNA-based metabolic processes and is a particularly well-documented response to both DNA damage and repair. By using both biological and polymer physics approaches, many of the contributing factors of chromatin motility have been elucidated. These include the intrinsic properties of chromatin, such as stiffness, as well as the loop modulators condensin
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Hepatocarcinogen 4-methylquinoline induced G:C to C:G transversions in the cII gene in the liver of lambda/lacZ transgenic mice (Muta™Mouse). Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-05-23 Yuki Kitamura,Takayoshi Suzuki,Arihiro Kohara,Ken-Ichi Saeki
We have previously reported that quinoline increased the mutation frequency of the cII gene in the liver of lambda/lacZ transgenic mice (Muta™Mouse), and G:C to C:G transversions were the molecular signature of quinoline-induced mutations. 4-Methylquinoline (4-MeQ) has the highest mutagenicity among quinoline and isomeric methylquinolines according to the Ames test using Salmonella typhimurium TA 100
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Characterization of an archaeal recombinase paralog that exhibits novel anti-recombinase activity. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-05-07 Corey Knadler,Michael Rolfsmeier,Antonia Vallejo,Cynthia Haseltine
The process of homologous recombination is heavily dependent on the RecA family of recombinases for repair of DNA double-strand breaks. These recombinases are responsible for identifying homologies and forming heteroduplex DNA between substrate ssDNA and dsDNA templates, activities that are modified by various accessory factors. In this work we describe the biochemical functions of the SsoRal2 recombinase
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Identification of nsSNPs of transcription factor E2F1 predisposing individuals to lung cancer and head and neck cancer. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-05-04 Sanjay Singh,Ragothaman M Yennamalli,Manish Gupta,Harish Changotra
E2Fs transcription factors family is involved in the G1/S transition and DNA replication and their deregulated expression have been reported in various human cancers. Studies have shown that the genetic variants of E2F1 family members play an important role in head and neck carcinogenesis. In this study, we predicted six highly deleterious nsSNPs (C227F, R252H, V295D, C298Y, R56W, and Y59C) of E2F1
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Mutations induced by Bleomycin, 4-nitroquinoline-1-oxide, and hydrogen peroxide in the rpoB gene of Escherichia coli: Perspective on Mutational Hotspots. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-03-30 Kristen Fernandez,Sara D'Souza,Jenny J Ahn,Summer Singh,Erin Mae Bacasen,Daniel Mashiach,Daniel Mishail,Timothy Kao,Jasmine Thai,Spring Hwang,Lekha Yaramada,Jeffrey H Miller
We report the mutational spectra in a segment of the E. coli rpoB gene of bleomycin (BLEO), 4-nitroquinoline-1-oxide (NQO), and hydrogen peroxide (H2O2). We compare these spectra with those of other mutagens and repair deficient strains in the same rpoB system, and review the key elements determining mutational hotspots and outline the questions that remain unanswered. We consider three tiers of hotspots
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Clinical potential of ATM inhibitors. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-03-06 Martin F Lavin,Abrey J Yeo
The protein defective in the human genetic disorder ataxia-telangiectasia, ATM, plays a central role in responding to DNA double strand breaks and other lesions to protect the genome against DNA damage and in this way minimize the risk of mutations that can lead to abnormal cellular behaviour. Its function in normal cells is to protect the cell against genotoxic stress but inadvertently it can assist
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Therapeutic opportunities for PLK1 inhibitors: Spotlight on BRCA1-deficiency and triple negative breast cancers. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-02-25 Iris Alejandra García,Cintia Garro,Elmer Fernandez,Gastón Soria
Polo-Like Kinases (PLKs) are central players of mitotic progression in Eukaryotes. Given the intimate relationship between cell cycle progression and cancer development, PLKs in general and PLK1 in particular have been thoroughly studied as biomarkers and potential therapeutic targets in oncology. The oncogenic properties of PLK1 overexpression across different types of human cancers are attributed
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Targeting DNA-PK in cancer. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-02-25 Giovanna Damia
DNA-dependent protein kinase (DNA-PK) is involved in many cellular pathways. It has a key role in the cellular response to DNA damage, in the repair of DNA double-strand break (DNA-DSBs) and as a consequence an important role in maintaining genomic integrity. In addition, DNA-PK has been shown to modulate transcription, to be involved in the development of the immune system and to protect telomeres
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Genome sequencing of ion-beam-induced mutants facilitates detection of candidate genes responsible for phenotypes of mutants in rice. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2020-02-21 Yutaka Oono,Hiroyuki Ichida,Ryouhei Morita,Shigeki Nozawa,Katsuya Satoh,Akemi Shimizu,Tomoko Abe,Hiroshi Kato,Yoshihiro Hase
Ion beams are physical mutagens used for plant and microbe breeding that cause mutations via a mechanism distinct from those of chemical mutagens or gamma rays. We utilized whole-exome sequencing of rice DNA in order to understand the properties of ion beam-induced mutations in a genome-wide manner. DNA libraries were constructed from selected carbon-ion-beam-induced rice mutants by capturing with
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Genomic health status assessed by a cytokinesis-block micronucleus cytome assay in a healthy middle-aged Korean population. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2017 Feb Cho, Nan Young; Kim, Kyung Woon; Kim, Kyoung Kon
The aim of this study was to determine the typical incidence of micronuclei (MNi) in the peripheral blood lymphocytes of healthy middle-aged Koreans using the cytokinesis-block micronucleus cytome (CBMN-Cyt) assay. Non-smoking, low-risk alcohol-drinking healthy Korean men and women aged 30 to 59 years were recruited. Participants were divided into three groups according to age, i.e., 30 to 39, 40 to
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Somatic mutations in cancer: Stochastic versus predictable. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2017 Feb Gold, Barry
The origins of human cancers remain unclear except for a limited number of potent environmental mutagens, such as tobacco and UV light, and in rare cases, familial germ line mutations that affect tumor suppressor genes or oncogenes. A significant component of cancer etiology has been deemed stochastic and correlated with the number of stem cells in a tissue, the number of times the stem cells divide
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In vitro protective effects of botryosphaeran, a (1-->3;1-->6)-beta-d-glucan, against mutagens in normal and tumor rodent cells. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2017 Feb Kerche-Silva, Leandra E; Colus, Ilce M S; Malini, Maressa; Mori, Mateus Prates; Dekker, Robert F H; Barbosa-Dekker, Aneli M
Botryosphaeran (BOT) is an exocellular beta-d-glucan (carbohydrate biopolymer) of the (1-->3;1-->6)-linked type produced by Botryosphaeria rhodina MAMB-05. The cytotoxic, mutagenic, genotoxic, and protective effects of this substance were evaluated in Chinese hamster lung fibroblasts (V79) and rat hepatocarcinoma cells (HTC) by the micronucleus test (MN) and the comet assay. BOT was not genotoxic in
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Nucleoplasmic bridges as a biomarker of DNA damage exposed to radon. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2017 Feb Meenakshi, C; Sivasubramanian, K; Venkatraman, B
Radon is a naturally occurring radionuclide in the environment, during decay it emits high linear energy transfer (LET) alpha particles. When radon exposure is accompanied by smoking it has been reported that lung cancer risk is higher. Blood samples were collected after prior consent, 25 smokers and 25 non smokers (only males) exposed in vitro to radon gas with doses ranging between 0.3-12.6mGy Ionizing
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Assessment of the genotoxicity of the tyrosine kinase inhibitor imatinib mesylate in cultured fish and human cells. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2017 Feb Novak, Matjaz; Zegura, Bojana; Nunic, Jana; Gajski, Goran; Geric, Marko; Garaj-Vrhovac, Vera; Filipic, Metka
The selective tyrosine kinase inhibitor imatinib mesylate (IM) is a widely used anticancer drug. Recent studies showing that IM can induce DNA and chromosomal damage in crustaceans and higher plants prompted us to re-examine its potential genotoxicity. IM was not mutagenic in the Ames assay (Salmonella typhimurium). Cytotoxicity and genotoxicity were evaluated in vitro in zebrafish (Danio rerio) liver
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Whole genome sequencing of mouse lymphoma L5178Y-3.7.2C (TK+/-) reveals millions of mutations and genetic markers. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2017 Feb McKinzie, Page B; Revollo, Javier R
The mouse lymphoma L5178Y-3.7.2C (TK+/-) cell line is extensively used in genetic toxicology to conduct the mouse lymphoma assay (MLA). The MLA is used to establish the mutagenic and clastogenic effects of chemicals and pharmaceuticals, and is one of the few genetic tests widely accepted by regulatory agencies throughout the world. Despite the extensive use and regulatory impact of L5178Y-3.7.2C (TK+/-)
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The DNA damage response of C. elegans affected by gravity sensing and radiosensitivity during the Shenzhou-8 spaceflight. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2017 Jan 0 Gao, Ying; Xu, Dan; Zhao, Lei; Sun, Yeqing
Space radiation and microgravity are recognized as primary and inevitable risk factors for humans traveling in space, but the reports regarding their synergistic effects remain inconclusive and vary across studies due to differences in the environmental conditions and intrinsic biological sensitivity. Thus, we studied the synergistic effects on transcriptional changes in the global genome and DNA damage
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Synergism of Dam, MutH, and MutS in methylation-directed mismatch repair in Escherichia coli. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2017 Jan 0 Hu, Changkun; Zhao, Yunqi; Sun, Huiyun; Yang, Yixin
DNA mismatch repair (MMR) is a critical mutation surveillance system for recognizing and repairing erroneous insertion, deletion, and disincorporation of base. Major components of mismatch repair system consist of MutH, MutL, and MutS. Dam methylates adenine to distinguish newly synthesized daughter strands from the parent strands. Employing a tyrosine-auxotrophic E. coli FX-11 strain, the mutation
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Influence of reduced glutathione on end-joining of DNA double-strand breaks: Cytogenetical and molecular approach. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2017 Jan Ghoshal, Nitin; Sharma, Sheetal; Banerjee, Atanu; Kurkalang, Sillarine; Raghavan, Sathees C; Chatterjee, Anupam
Radiation induced DNA double-strand breaks (DSB) are the major initial lesions whose misrejoining may lead to exchange aberrations. However, the role of glutathione (GSH), a major cellular thiol, in regulating cell's sensitivity to DNA damaging agents is not well understood. Influence of endogenous GSH on the efficiency of X-rays and bleomycin (Blem) induced DNA DSBs end-joining has been tested here
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A novel type of gene interaction in D. melanogaster. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2017 Jan Chadov, B F; Chadova, E V; Fedorova, N B
The genes interact according to classical mechanisms, namely, complementation, modification, polymery, and epistasis, in the cells and organisms carrying these genes. Here we describe a novel type of gene interaction when the interacting genes reside in parents, whereas the interaction event takes place in their progenies lacking these genes. The conditional mutations in the D. melanogaster male X
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Ganoderma lucidum total triterpenes induce apoptosis in MCF-7 cells and attenuate DMBA induced mammary and skin carcinomas in experimental animals. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2017 Jan Smina, T P; Nitha, B; Devasagayam, T P A; Janardhanan, K K
Ganoderma lucidum total triterpenes were evaluated for its apoptosis-inducing and anti-cancer activities. Cytotoxicity and pro-apoptotic effect of total triterpenes were evaluated in human breast adenocarcinoma (MCF-7) cell line using MTT assay and DNA fragmentation analysis. Total triterpenes induced apoptosis in MCF-7 cells by down-regulating the levels of cyclin D1, Bcl-2, Bcl-xL and also by up-regulating
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Assessing the genotoxicity of two commonly occurring byproducts of water disinfection: Chloral hydrate and bromal hydrate. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2017 Jan Manasfi, Tarek; De Meo, Michel; Di Giorgio, Carole; Coulomb, Bruno; Boudenne, Jean-Luc
Water disinfection treatments result in the formation of disinfection byproducts (DBPs) that have been linked to adverse human health outcomes including higher incidence of bladder and colorectal cancer. However, data about the genotoxicity of DBPs is limited to only a small fraction of compounds. Chloral hydrate (CH) and bromal hydrate (BH) are two trihaloacetaldehydes commonly detected in disinfected
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Increased methylation of repetitive elements and DNA repair genes is associated with higher DNA oxidation in children in an urbanized, industrial environment. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2017 Jan Alvarado-Cruz, Isabel; Sanchez-Guerra, Marco; Hernandez-Cadena, Leticia; De Vizcaya-Ruiz, Andrea; Mugica, Violeta; Pelallo-Martinez, Nadia Azenet; Solis-Heredia, Maria de Jesus; Byun, Hyang-Min; Baccarelli, Andrea; Quintanilla-Vega, Betzabet
DNA methylation in DNA repair genes participates in the DNA damage regulation. Particulate matter (PM), which has metals and polycyclic aromatic hydrocarbons (PAHs) adsorbed, among others has been linked to adverse health outcomes and may modify DNA methylation. To evaluate PM exposure impact on repetitive elements and gene-specific DNA methylation and DNA damage, we conducted a cross-sectional study
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Effects of chronic restraint-induced stress on radiation-induced chromosomal aberrations in mouse splenocytes. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2017 Jan Katsube, Takanori; Wang, Bing; Tanaka, Kaoru; Ninomiya, Yasuharu; Vares, Guillaume; Kawagoshi, Taiki; Shiomi, Naoko; Kubota, Yoshihisa; Liu, Qiang; Morita, Akinori; Nakajima, Tetsuo; Nenoi, Mitsuru
Both ionizing radiation (IR) and psychological stress (PS) cause detrimental effects on humans. A recent study showed that chronic restraint-induced PS (CRIPS) diminished the functions of Trp53 and enhanced radiocarcinogenesis in Trp53-heterozygous (Trp53+/-) mice. These findings had a marked impact on the academic field as well as the general public, particularly among residents living in areas radioactively
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Upregulation of NRF2 through autophagy/ERK 1/2 ameliorates ionizing radiation induced cell death of human osteosarcoma U-2 OS. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2017 Jan Chen, Ni; Zhang, Rui; Konishi, Teruaki; Wang, Jun
The antioxidative response mediated by transcription factor NRF2 is thought to be a pivotal cellular defense system against various extrinsic stresses. It has been reported that activation of the NRF2 pathway confers cells with resistance to ionizing radiation-induced damage. However, the underlying mechanism remains largely unknown. In the current research, it was found that alpha-particle radiation
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Biomonitoring of gasoline station attendants exposed to benzene: Effect of gender. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2017 Jan Moro, Angela M; Brucker, Natalia; Charao, Mariele F; Baierle, Marilia; Sauer, Elisa; Goethel, Gabriela; Barth, Anelise; Nascimento, Sabrina N; Gauer, Bruna; Durgante, Juliano; Amaral, Beatriz S; Neto, Francisco R A; Gioda, Adriana; Garcia, Solange C
Women are employed in increasing numbers as gasoline station attendants, a work category with risk of exposure to benzene. We have assessed the effect of gender on biomarkers of occupational benzene exposure. Gasoline station attendants (20 men and 20 women) and 40 control individuals (20 men and 20 women) with no history of occupational benzene exposure were evaluated. Benzene exposure was monitoring
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Sample size determination for the fluctuation experiment. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2016 Dec 1 Zheng, Qi
The Luria-Delbruck fluctuation experiment protocol is increasingly employed to determine microbial mutation rates in the laboratory. An important question raised at the planning stage is "How many cultures are needed?" For over 70 years sample sizes have been determined either by intuition or by following published examples where sample sizes were chosen intuitively. This paper proposes a practical
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Genetic polymorphisms in 19q13.3 genes associated with alteration of repair capacity to BPDE-DNA adducts in primary cultured lymphocytes. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2016 Dec Xiao, Mingyang; Xiao, Sha; Straaten, Tahar van der; Xue, Ping; Zhang, Guopei; Zheng, Xiao; Zhang, Qianye; Cai, Yuan; Jin, Cuihong; Yang, Jinghua; Wu, Shengwen; Zhu, Guolian; Lu, Xiaobo
Benzo[a]pyrene(B[a]P), and its ultimate metabolite Benzo[a]pyrene 7,8-diol 9,10-epoxide (BPDE), are classic DNA damaging carcinogens. DNA damage in cells caused by BPDE is normally repaired by Nucleotide Excision Repair (NER) and Base Excision Repair (BER). Genetic variations in NER and BER can change individual DNA repair capacity to DNA damage induced by BPDE. In the present study we determined the
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The mutagenic assessment of an electronic-cigarette and reference cigarette smoke using the Ames assay in strains TA98 and TA100. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2016 Dec Thorne, D; Crooks, I; Hollings, M; Seymour, A; Meredith, C; Gaca, M
Salmonella typhimurium strains TA98 and TA100 were used to assess the mutagenic potential of the aerosol from a commercially available, rechargeable, closed system electronic-cigarette. Results obtained were compared to those for the mainstream smoke from a Kentucky reference (3R4F) cigarette. Two different test matrices were assessed. Aerosol generated from the e-cigarette was trapped on a Cambridge
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DNA replication after mutagenic treatment in Hordeum vulgare. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2016 Dec Kwasniewska, Jolanta; Kus, Arita; Swoboda, Monika; Braszewska-Zalewska, Agnieszka
The temporal and spatial properties of DNA replication in plants related to DNA damage and mutagenesis is poorly understood. Experiments were carried out to explore the relationships between DNA replication, chromatin structure and DNA damage in nuclei from barley root tips. We quantitavely analysed the topological organisation of replication foci using pulse EdU labelling during the S phase and its
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The insecticide spinosad induces DNA damage and apoptosis in HEK293 and HepG2 cells. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2016 Dec Yang, Mingjun; Xiang, Guanggang; Li, Diqiu; Zhang, Yang; Xu, Wenping; Tao, Liming
Spinosad, a pesticide acting on the central nervous system of insects, is classified as a pesticide with reduced risk. However, spinosad-induced toxicological effects on non-target organisms must not be ignored. This study aimed to evaluate the cytotoxicity and potential genotoxicity of spinosad in HEK293 and HepG2 cell lines. The results showed that spinosad caused a concentration- and time-dependent
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Water-soluble and organic extracts of airborne particulate matter induce micronuclei in human lung epithelial A549 cells. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2016 Dec Palacio, Isabel C; Barros, Silvia B M; Roubicek, Deborah A
The in vitro genotoxic effects of organic and water-soluble fractions of airborne particulate matter (PM10) with the cytokinesis blocked micronucleus (MN) test in human alveolar carcinoma cells A549 were investigated. Samples were collected in three different sites of Sao Paulo State, Brazil, and fifteen soluble metals and the sixteen EPAs priority polycyclic aromatic hydrocarbons (PAH) were chemically
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Evaluation of a single-dose PIGRET assay for cisplatin in rats compared with the RBC Pig-a assay. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2016 Nov 1 Suzuki, Yuta; Goto, Ken; Nakayama, Yoshihiro; Saratani, Masatoshi; Takata, Takuya; Okamoto, Takezo; Okazaki, Shuzo
As a part of a collaborative study of the Pig-a assay by the Mammalian Mutagenicity Study Group of the Japanese Environmental Mutagen Society, a genotoxicity study on cisplatin was performed using red blood cell (RBC) Pig-a and PIGRET assays. The dose levels were set at 0 (vehicle, physiological saline), 0.5, 1, and 2 mg/kg, and cisplatin was administered intravenously once to male F344 rats. The RBC
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Evaluation of red blood cell Pig-a assay and PIGRET assay in rats using chlorambucil. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2016 Nov 1 Maeda, Akihisa; Takahashi, Kei; Tsuchiyama, Hiromi; Oshida, Keiyu
The Pig-a assay is a novel method to assess the in vivo mutagenicity of compounds, and it is expected to be useful for the detection of genotoxicity. In this study, to assess the performance of the Pig-a assay targeting red blood cells (RBCs; RBC Pig-a assay) and reticulocytes (RETs; PIGRET assay), chlorambucil, which is a genotoxicant, was orally administered to male rats once at 10, 20 and 40mg/kg
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Evaluation of the RBC Pig-a assay and the PIGRET assay using benzo[a]pyrene in rats. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2016 Nov 1 Kikuzuki, Ryuta; Sato, Haruka; Fujiwara, Ai; Takahashi, Tomoko; Ogiwara, Yosuke; Sugiura, Mihoko
The red blood cell (RBC) Pig-a assay has the potential to detect the in vivo mutagenicity of chemicals. Recently, use of the Pig-a assay with reticulocytes (the PIGRET assay) reportedly enabled the in vivo mutagenicity of chemicals to be detected earlier than using the RBC Pig-a assay. To evaluate whether the PIGRET assay is useful and effective as a short-term test, compared with the RBC Pig-a assay
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Evaluation for a mutagenicity of aristolochic acid by Pig-a and PIGRET assays in rats. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2016 Nov 1 Koyama, Naomi; Yonezawa, Yutaka; Nakamura, Michi; Sanada, Hisakazu
The Pig-a assay, which uses the endogenous phosphatidylinositol glycan, class A gene (Pig-a) as a reporter of mutation, has been developed as a method for evaluating in vivo mutagenicity. Pig-a gene mutation can be detected by identifying the presence of CD59, the glycosylphosphatidylinositol anchor protein, on the surface of erythrocytes (RBC Pig-a assay) and reticulocytes (PIGRET assay). The International
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Results of rat Pig-a/PIGRET assay with a single dose regimen of 1,3-propane sultone and 2-acetyl aminofluorene. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2016 Nov 1 Shigano, Miyuki; Ishii, Nana; Takashima, Rie; Harada, Hideki; Takasawa, Hironao; Hamada, Shuichi
The Pig-a assay is a useful in vivo mutation detecting test and is easier to perform than the in vivo transgenic mutation assay. This assay is now recognized to be able to detect a number of mutagenic chemicals administered to rats in sub-acute or sub-chronic dose studies. The present investigation was conducted to evaluate the usefulness of peripheral blood Pig-a assays with total red blood cells
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Evaluation of the PIGRET assay as a short-term test using a single dose of diethylnitrosamine. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2016 Nov 1 Wada, Kunio; Nishino, Risako; Fukuyama, Tomoki; Matsumoto, Kyomu
The PIGRET assay, which was developed as the Pig-a assay in reticulocytes, can detect mutagenicity of compounds earlier than the Pig-a assay in total red blood cells (RBC; RBC Pig-a assay). The usefulness of the PIGRET assay as a short-term test has been confirmed in a collaborative study in Japan with 24 chemicals. One of these chemicals, diethylnitrosamine (DEN), which mainly induces liver tumors
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Evaluation of the PIGRET assay in rats by single oral dosing with azidothymidine. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2016 Nov 1 Sanada, Hisakazu; Ohsumi, Tomoka; Koyama, Naomi; Miyashita, Taishi; Hashimoto, Kazuto
In vivo phosphatidylinositol glycan, class A (Pig-a) gene mutation assay using peripheral blood is known to be a novel and useful tool to evaluate the mutagenicity of compounds. Recently, the rat PIGRET assay which is an improved method for measuring Pig-a mutant cells in reticulocytes with magnetic enrichment of CD71 positive cells has been developed. Several reports showed that the PIGRET assay could
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Red blood cell Pig-a assay and PIGRET assay in rats with azathioprine. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2016 Nov 1 Yoshida, Ikuma; Matsumoto, Akemi; Sakai, Yumi; Harada, Yumiko; Hashizume, Tsuneo
A new in vivo gene mutation assay has been developed based on the phosphatidylinositol glycan anchor biosynthesis, Class A gene (Pig-a in rodents) as an endogenous reporter. Using this Pig-a assay, the in vivo mutagenicity of a single dose of azathioprine (Aza) was investigated in red blood cells (RBC Pig-a assay) and reticulocytes (PIGRET) of rats. Eight-week old male rats were orally dosed once with
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Evaluation of mutagenicity of acrylamide using RBC Pig-a and PIGRET assays by single peroral dose in rats. Mutat. Res. Fund. Mol. Mech. Mutagen. (IF 2.463) Pub Date : 2016 Nov 1 Horibata, Katsuyoshi; Ukai, Akiko; Honma, Masamitsu
The Pig-a gene mutation assay, a powerful tool for evaluating in vivo genotoxicity, is based on flow cytometric enumeration of red blood cells (RBCs), which are deficient in glycosylphosphatidylinositol anchored proteins caused by mutation(s) in the Pig-a gene. Various approaches for measuring cells with mutated Pig-a gene have been developed. The Pig-a assay targeting concentrated reticulocytes -