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Site-specific protein labeling strategies for super-resolution microscopy Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2024-03-14 Made Budiarta, Marcel Streit, Gerti Beliu
Super-resolution microscopy (SRM) has transformed our understanding of proteins' subcellular organization and revealed cellular details down to nanometers, far beyond conventional microscopy. While localization precision is independent of the number of fluorophores attached to a biomolecule, labeling density is a decisive factor for resolving complex biological structures. The average distance between
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Recent advances in ratiometric fluorescence imaging of enzyme activity in vivo Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2024-03-07 Emily D. Cosco, Matthew Bogyo
Among molecular imaging modalities that can monitor enzyme activity optical imaging provides sensitive, molecular-level information at low-cost using safe and non-ionizing wavelengths of light. Yet, obtaining quantifiable optical signals poses significant challenges. Benchmarking using ratiometric signals can overcome dependence on dosing, illumination variability, and pharmacokinetics to provide quantitative
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Lumos maxima – How robust fluorophores resist photobleaching? Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2024-03-02 Yuan Zhang, Jing Ling, Tianyan Liu, Zhixing Chen
Fluorescent dyes synergize with advanced microscopy for researchers to investigate the location and dynamic processes of biomacromolecules with high spatial and temporal resolution. However, the instability of fluorescent dyes, including photobleaching and photoconversion, represent fundamental limits for super-resolution and time-lapse imaging. In this review, we discuss the latest advances in improving
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Versatile roles of cysteine persulfides in tumor biology Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2024-02-28 Klaudia Borbényi-Galambos, Ágnes Czikora, Katalin Erdélyi, Péter Nagy
Rewiring the transsulfuration pathway is recognized as a rapid adaptive metabolic response to environmental conditions in cancer cells to support their increased cysteine demand and to produce Reactive Sulfur Species (RSS) including hydrogen sulfide (HS) and cysteine persulfide. This can directly (via RSS) or indirectly (by supplying Cys) trigger chemical or enzyme catalyzed persulfidation on critical
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D-Amino acid oxidase-derived chemogenetic oxidative stress: Unraveling the multi-omic responses to in vivo redox stress Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2024-02-27 Fotios Spyropoulos, Thomas Michel
Chemogenetic approaches have been developed to define the mechanisms whereby the intracellular oxidant hydrogen peroxide (HO) modulates both physiological and pathological responses. Recombinant yeast D-amino acid oxidase (DAAO) can be exploited to modulate H₂O₂ in target cells and tissues. In vitro studies using cultured cells expressing recombinant DAAO have provided critical new information on the
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A new era of cysteine proteomics – Technological advances in thiol biology Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2024-02-20 Nils Burger, Edward T. Chouchani
Cysteines are amenable to a diverse set of modifications that exhibit critical regulatory functions over the proteome and thereby control a wide range of cellular processes. Proteomic technologies have emerged as a powerful strategy to interrogate cysteine modifications across the proteome. Recent advancements in enrichment strategies, multiplexing capabilities and increased analytical sensitivity
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Biochemistry of the hypoxia-inducible factor hydroxylases Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2024-02-07 Giorgia Fiorini, Christopher J. Schofield
The hypoxia-inducible factors are α,β-heterodimeric transcription factors that mediate the chronic response to hypoxia in humans and other animals. Protein hydroxylases belonging to two different structural subfamilies of the Fe(II) and 2-oxoglutarate (2OG)-dependent oxygenase superfamily modify HIFα. HIFα prolyl-hydroxylation, as catalysed by the PHDs, regulates HIFα levels and, consequently, α,β-HIF
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Metabolomics-guided utilization of beneficial microbes for climate-resilient crops Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2024-01-29 Oluwaseyi Samuel Olanrewaju, Bernard R. Glick, Olubukola Oluranti Babalola
In the rhizosphere, plants and microbes communicate chemically, especially under environmental stress. Over millions of years, plants and their microbiome have coevolved, sharing various chemicals, including signaling molecules. This mutual exchange impacts bacterial communication and influences plant metabolism. Inter-kingdom signal crosstalk affects bacterial colonization and plant fitness. Beneficial
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New frontiers in sulfur and selenium chemical biology Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2024-01-25 Kate S. Carroll
Abstract not available
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Electrophilic metabolites targeting the KEAP1/NRF2 partnership Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2024-01-18 Albena T. Dinkova-Kostova, Henriikka Hakomäki, Anna-Liisa Levonen
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Peroxisomal hydrogen peroxide signaling: A new chapter in intracellular communication research Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2024-01-17 Marc Fransen, Celien Lismont
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Recent antibacterial carbohydrate-based prodrugs, drugs and delivery systems to overcome antimicrobial resistance Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2024-01-13 Catarina Maria, Ana M. de Matos, Amélia P. Rauter
Antimicrobial resistance is an increasing phenomenon that is threatening global health. Tuberculosis causative bacteria and several resistant and multidrug-resistant bacteria are widely spread and listed by the World Health Organization as global priorities for research and development. Hence, new antibacterial agents with new modes of action are urgently required. In this context, carbohydrate-based
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Recent progress in the synthesis of glycosphingolipids Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2024-01-06 Hiromune Ando, Naoko Komura
To accelerate the biological study and application of the diverse functions of glycosphingolipids (GSLs), the production of structurally defined GSLs has been greatly demanded. In this review, we focus on the recent developments in the chemical and chemoenzymatic synthesis of GSLs. In the chemical synthesis section, the syntheses based on glucosyl ceramide cassette, late-stage sialylation, and diversity-oriented
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Recent research progress in glycosylphosphatidylinositol-anchored protein biosynthesis, chemical/chemoenzymatic synthesis, and interaction with the cell membrane Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2024-01-04 Zhongwu Guo, Sayan Kundu
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Recent advances in antibody glycoengineering for the gain of functions Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2024-01-03 Zhi Liu, Xiangman Zou, Feng Tang, Wei Huang
Glycans play important roles in antibody functions, and antibody glycoengineering has long been an important research field. Here, we summarize the significant strategies of antibody glycoengineering, including expressed antibody glycoengineering in mammalian cell expression systems, chemo-enzymatic antibody glycoengineering, and yeast expression system-based antibody engineering, as well as the applications
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Recent chemical synthesis and immunological evaluation of glycans related to bacterial lipopolysaccharides Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2024-01-03 Chunjun Qin, Guangzong Tian, Jing Hu, Xiaopeng Zou, Jian Yin
O-Antigens and core oligosaccharides from bacterial lipopolysaccharides (LPS) are often structurally unique and immunologically active, have become attractive targets in the development of antibacterial vaccines. Structurally well-defined and pure oligosaccharides can be used in identifying protective epitopes of the carbohydrate antigens, which is important for the design of an effective vaccine.
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Recent advances in enzymatic and chemoenzymatic synthesis of N- and O-glycans Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-12-22 Zhi-Fei Hu, Kan Zhong, Hongzhi Cao
Glycosylation is one of the most common post-translational modifications of proteins, which plays essential roles in regulating the biological functions of proteins. Efficient and versatile methods for the synthesis of homogeneous and well-defined N- and O-glycans remain an urgent need for biological studies and biomedical applications. Despite their structural complexity, tremendous progress has been
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Synthesis and application of bacterial exopolysaccharides Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-12-21 Gijs Ruijgrok, Dung-Yeh Wu, Herman S. Overkleeft, Jeroen D.C. Codée
Exopolysaccharides are produced and excreted by bacteria in the generation of biofilms to provide a protective environment. These polysaccharides are generally generated as heterogeneous polymers of varying length, featuring diverse substitution patterns. To obtain well-defined fragments of these polysaccharides, organic synthesis often is the method of choice, as it allows for full control over chain
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Blurred lines: Crossing the boundaries between the chemical exposome and the metabolome Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-12-11 Cristina Balcells, Yitao Xu, Rubén Gil-Solsona, Léa Maitre, Pablo Gago-Ferrero, Hector C. Keun
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Editorial overview: Glycobiology (2023) Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-11-11 Matthew S. Macauley
Abstract not available
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Small-molecule tools for YEATS domain proteins Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-11-03 Michael A. Erb
Chromatin reader domains are protein folds that bind to post-translational modifications of histones and other chromatin-associated proteins. Compared to other families of reader domains, the discovery that YEATS domains bind to acylated lysines is relatively recent. Four human proteins harbor a YEATS domain, and each is present in protein complexes that regulate chromatin and transcription (ENL, AF9
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Recent advances in chemical synthesis of O-linked glycopeptides and glycoproteins: An advanced synthetic tool for exploring the biological realm Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-10-26 Jie Zhao, Farong Ye, Ping Huang, Ping Wang
Glycoproteins play crucial roles in various biological processes. To investigate the relationship between glycan structure and function, researchers have employed various chemical methods to precisely synthesize homogeneous O-glycoproteins. This review summarizes the recent progress of their synthetic strategies, highlighting the significant advancements in this area.
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Strategies for quantifying the enzymatic activities of glycoside hydrolases within cells and in vivo Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-10-17 Matthew C. Deen, Pierre-André Gilormini, David J. Vocadlo
Within their native milieu of the cell, the activities of enzymes are controlled by a range of factors including protein interactions and post-translational modifications. The involvement of these factors in fundamental cell biology and the etiology of diseases is stimulating interest in monitoring enzyme activities within tissues. The creation of synthetic substrates, and their use with different
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Nutritional metabolomics: Recent developments and future needs Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-10-05 Maaria Kortesniemi, Stefania Noerman, Anna Kårlund, Jasmin Raita, Topi Meuronen, Ville Koistinen, Rikard Landberg, Kati Hanhineva
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Metabolic interventions to enhance immunotherapy and targeted therapy efficacy in advanced colorectal cancer Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-10-06 Helena Oliveres, Marta Cascante, Joan Maurel
Current standard-of-care for metastatic colorectal cancer patients includes chemotherapy and anti-angiogenic or anti-epidermal growth factor receptor for microsatellite stable tumors and pembrolizumab for microsatellite instable tumors. However, despite the available therapies, the prognosis remains poor. In recent years, new drugs combined with immune checkpoint inhibitors have been tested in microsatellite
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Immunomodulatory roles of PARPs: Shaping the tumor microenvironment, one ADP-ribose at a time Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-10-04 Deja M. Brooks, Sudarshan Anand, Michael S. Cohen
PARPs encompass a small yet pervasive group of 17 enzymes that catalyze a post-translational modification known as ADP-ribosylation. PARP1, the founding member, has received considerable focus; however, in recent years, the spotlight has shifted to other members within the PARP family. In this opinion piece, we first discuss surprising findings that some FDA-approved PARP1 inhibitors activate innate
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Global approaches for protein thiol redox state detection Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-10-03 Lisa R. Knoke, Lars I. Leichert
Due to its nucleophilicity, the thiol group of cysteine is chemically very versatile. Hence, cysteine often has important functions in a protein, be it as the active site or, in extracellular proteins, as part of a structural disulfide. Within the cytosol, cysteines are typically reduced. But the nucleophilicity of its thiol group makes it also particularly prone to post-translational oxidative modifications
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Editorial overview: Imaging metal ions in biological systems Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-10-01 Daniela Buccella, Christoph J. Fahrni
Abstract not available
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Recent advances in mass spectrometry-based methods to investigate reversible cysteine oxidation Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-09-28 Evan R. Stair, Leslie M. Hicks
The post-translational modification of cysteine to diverse oxidative states is understood as a critical cellular mechanism to combat oxidative stress. To study the role of cysteine oxidation, cysteine enrichments and subsequent analysis via mass spectrometry are necessary. As such, technologies and methods are rapidly developing for sensitive and efficient enrichments of cysteines to further explore
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Recent chemical synthesis of plant polysaccharides Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-09-14 Xiufang Wang, Guozhi Xiao
Here, chemical syntheses of long, branched and complex glycans over 10-mer from plants are summarized, which highlights amylopectin 20-mer from starch, 17-mer from carthamus tinctorius, α-glucan 30-mer from Longan, 19-mer from psidium guajava and 11-mer from dendrobium huoshanense. The glycans assembly strategies, protecting groups utilization and glycosylation methods discussed here will inspire the
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Navigating the terrain of protein aggregation and phase separation - A chemical biology perspective Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-09-04 Cong Liu, Xin Zhang
Abstract not available
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Recent advances in Zn2+ imaging: From organelles to in vivo applications Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-08-24 Hongbao Fang, Yaheng Li, Xiuzhi Yang, Yuncong Chen, Zijian Guo, Weijiang He
Zn2+ is involved in various physiological and pathological processes in living systems. Monitoring the dynamic spatiotemporal changes of Zn2+ levels in organelles, cells, and in vivo is of great importance for the investigation of the physiological and pathological functions of Zn2+. However, this task is quite challenging since Zn2+ in living systems is present at low concentrations and undergoes
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How nature incorporates sulfur and selenium into bioactive natural products Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-08-18 Xiaoyan Chen, Bo Li
Living organisms have evolved various strategies to incorporate sulfur and selenium into bioactive natural products. These chalcogen-containing compounds serve important and diverse biological functions for their producers and many of them are essential medicines against infectious diseases and cancer. We review recent advances in the biosynthesis of some sulfur/selenium-containing natural products
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A translational blueprint for developing intraoperative imaging agents via radiopharmaceutical-guided drug design Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-08-10 Teresa E. Sullivan, Servando Hernandez Vargas, Sukhen C. Ghosh, Solmaz AghaAmiri, Naruhiko Ikoma, Ali Azhdarinia
Cancer imaging is a rapidly evolving field due to the discovery of novel molecular targets and the availability of corresponding techniques to detect them with high precision, accuracy, and sensitivity. Nuclear medicine is the most widely used molecular imaging modality and has a growing toolkit of clinically used radiopharmaceuticals that enable whole-body tumor visualization, staging, and treatment
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Virus-assisted directed evolution of biomolecules Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-08-03 Delilah Jewel, Quan Pham, Abhishek Chatterjee
Directed evolution is a powerful technique that uses principles of natural evolution to enable the development of biomolecules with novel functions. However, the slow pace of natural evolution does not support the demand for rapidly generating new biomolecular functions in the laboratory. Viruses offer a unique path to design fast laboratory evolution experiments, owing to their innate ability to evolve
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Advanced imaging techniques for studying protein phase separation in living cells and at single-molecule level Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-07-29 Gemechu Mekonnen, Nathalie Djaja, Xincheng Yuan, Sua Myong
Protein-protein and protein-RNA interactions are essential for cell function and survival. These interactions facilitate the formation of ribonucleoprotein complexes and biomolecular condensates via phase separation. Such assembly is involved in transcription, splicing, translation and stress response. When dysregulated, proteins and RNA can undergo irreversible aggregation which can be cytotoxic and
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Luminescent lanthanide probes for cations and anions: Promises, compromises, and caveats Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-07-28 Thibaut L.M. Martinon, Valérie C. Pierre
The long luminescence lifetimes and sharp emission bands of luminescent lanthanide complexes have long been recognized as invaluable strengths for sensing and imaging in complex aqueous biological or environmental media. Herein we discuss the recent developments of these probes for sensing metal ions and, increasingly, anions. Underappreciated in the field, buffers and metal hydrolysis influence the
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Peptide-based molecules for the disruption of bacterial Hsp70 chaperones Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-07-27 Aweon Richards, Tania J. Lupoli
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Multimodal and multiscale correlative elemental imaging: From whole tissues down to organelles Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-07-22 Stéphane Roudeau, Asuncion Carmona, Richard Ortega
Chemical elements, especially metals, play very specific roles in the life sciences. The implementation of correlative imaging methods, of elements on the one hand and of molecules or biological structures on the other hand, is the subject of recent developments. The most commonly used spectro-imaging techniques for metals are synchrotron-induced X-ray fluorescence, mass spectrometry and fluorescence
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The influence of alkylating agents on sulfur–sulfur bonds in per- and polysulfides Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-07-18
Per- and polysulfides are sulfane sulfur species produced inside living cells, in organisms as diverse as bacteria, plants and humans, but their biological roles remain to be fully understood. Unfortunately, due to their reactivity, per- and polysulfides are easily altered, interconverted or lost during the processing and analysis of biological material. Thus, all current analytical methods make use
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Recent advances towards mass spectrometry-based clinical lipidomics Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-07-18
The objective of this review is to provide a comprehensive summary of the latest methodological advancements and emerging patterns in utilizing lipidomics in clinical research.In this review, we assess the recent advancements in lipidomics methodologies that exhibit high levels of selectivity and sensitivity, capable of generating numerous molecular lipid species from limited quantities of biological
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The redox riddle of selenium sulfide Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-07-17
Selenium sulfide, in analogy with selenium dioxide, is often considered as SeS2. At closer inspection, however, selenium sulfide represents a large family of rather complicated molecules which differ depending on the mode of preparation. Together, these compounds share extraordinarily low solubility in virtually any solvent with a biological activity rather impressive for such simple molecules. The
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Characteristics of genetic tags for correlative light and electron microscopy Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-07-13
Fluorescence microscopy is indispensable in live cell studies of fluorescently-labeled proteins, but has limited resolution and context. Electron microscopy offers high-resolution imaging of cellular ultrastructure, including membranes, organelles, and other nanoscale features. However, identifying proteins by EM remains a substantial challenge. There is potential to combine the strengths of both FM
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Improving precision surgery: A review of current intraoperative nerve tissue fluorescence imaging Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-07-14
Iatrogenic nerve injury represents one of the most feared surgical complications and remains a major morbidity across many surgical specialties. Currently, no clinically approved technique can directly enhance intraoperative nerve visualization, where intraoperative nerve identification continues to challenge even experienced surgeons. Fluorescence-guided surgery (FGS) has been successfully integrated
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The many facets of sulfur incorporation in natural product biosynthesis Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-07-12
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Analytical techniques in chemical biology Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-07-11
Abstract not available
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The role of metals in the next generation of anticancer therapeutics Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-07-12
Abstract not available
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Activity-based protein profiling in microbes and the gut microbiome Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-07-08 Lin Han, Pamela V. Chang
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Spatial metabolomics principles and application to cancer research Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-07-04 Mélanie Planque, Sebastian Igelmann, Ana Margarida Ferreira Campos, Sarah-Maria Fendt
Mass spectrometry imaging (MSI) is an emerging technology in cancer metabolomics. Desorption electrospray ionization (DESI) and matrix-assisted laser desorption ionization (MALDI) MSI are complementary techniques to identify hundreds of metabolites in space with close to single-cell resolution. This technology leap enables research focusing on tumor heterogeneity, cancer cell plasticity, and the communication
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New advances in cross-linking mass spectrometry toward structural systems biology Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-07-03 Clinton Yu, Lan Huang
Elucidating protein–protein interaction (PPI) networks and their structural features within cells is central to understanding fundamental biology and associations of cell phenotypes with human pathologies. Owing to technological advancements during the last decade, cross-linking mass spectrometry (XL-MS) has become an enabling technology for delineating interaction landscapes of proteomes as they exist
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Activity-based probes in pathogenic bacteria: Investigating drug targets and molecule specificity Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-07-03 Hannah K. Lembke, Erin E. Carlson
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Sensing and regulation of reactive sulfur species (RSS) in bacteria Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-07-01 David P. Giedroc, Giuliano T. Antelo, Joseph N. Fakhoury, Daiana A. Capdevila
The infected host deploys generalized oxidative stress caused by small inorganic reactive molecules as antibacterial weapons. An emerging consensus is that hydrogen sulfide (H2S) and forms of sulfur with sulfur–sulfur bonds termed reactive sulfur species (RSS) provide protection against oxidative stressors and antibiotics, as antioxidants. Here, we review our current understanding of RSS chemistry
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How do metabolic processes age: Evidence from human metabolomic studies Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-06-30 Oliver Robinson, ChungHo E. Lau
Metabolomics, the global profiling of small molecules in the body, has emerged as a promising analytical approach for assessing molecular changes associated with ageing at the population level. Understanding root metabolic ageing pathways may have important implications for managing age-related disease risk. In this short review, relevant studies published in the last few years that have made valuable
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Emergence of (hydro)persulfides as suppressors of lipid peroxidation and ferroptotic cell death Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-06-24 Zijun Wu, Uladzimir Barayeu, Danny Schilling, Tobias P. Dick, Derek A. Pratt
Recognition of the prevalence of hydropersulfides (RSSH) and characterization of their enhanced two-electron reactivity relative to thiols have led to their implication in maintaining cellular redox homeostasis, in addition to other potential roles. Recent attention on the one-electron reactivity of RSSH has uncovered their potent radical-trapping antioxidant activity, which enables them to inhibit
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The multifaceted nature of peroxiredoxins in chemical biology Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-06-27 Sebastián F. Villar, Gerardo Ferrer-Sueta, Ana Denicola
Peroxiredoxins (Prx), thiol-dependent peroxidases, were first identified as H2O2 detoxifiers, and more recently as H2O2 sensors, intermediates in redox-signaling pathways, metabolism modulators, and chaperones. The multifaceted nature of Prx is not only dependent on their peroxidase activity but also strongly associated with specific protein–protein interactions that are being identified, and where
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Small molecule fluorescent probes for the study of protein phase separation Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-06-24 Tze Cin Owyong, Jiamin Zhao, Yuning Hong
Liquid–liquid phase separation (LLPS) and liquid–solid phase transitions (LSPT) play crucial roles in biological systems, including sorting biomolecules, facilitate the transport of substrates for assembly, and accelerate the formation of metabolic and signaling complexes. Efforts towards improved characterization and quantification of phase separated species remain of outstanding interest and priority
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Recent advances in developing degraders & inhibitors of lysine methyltransferases Curr. Opin. Chem. Biol. (IF 7.8) Pub Date : 2023-06-26 Julia Velez, H. Ümit Kaniskan, Jian Jin
Over the last several decades, there has been continued interest in developing novel therapeutic approaches targeting protein lysine methyltransferases (PKMTs). Along with PKMT inhibitors, targeted protein degradation (TPD) has emerged as a promising strategy to attenuate aberrant PKMT activity. Particularly, proteolysis targeting chimeras (PROTACs) effectively eliminate PKMTs of interest, suppressing