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Crosstalk between Signaling Pathways and Energy Metabolism in Pluripotency. Int. J. Stem Cells (IF 2.3) Pub Date : 2024-03-18 Keun-Tae Kim, Seong-Min Kim, Hyuk-Jin Cha
The sequential change from totipotency to multipotency occurs during early mammalian embryo development. However, due to the lack of cellular models to recapitulate the distinct potency of stem cells at each stage, their molecular and cellular characteristics remain ambiguous. The establishment of isogenic naïve and primed pluripotent stem cells to represent the pluripotency in the inner cell mass
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Inducing Pluripotency in Somatic Cells: Historical Perspective and Recent Advances. Int. J. Stem Cells (IF 2.3) Pub Date : 2024-01-29 Junmyeong Park, Jueun Kim, Borami Shin, Hans R Schöler, Johnny Kim, Kee-Pyo Kim
Inducing pluripotency in somatic cells is mediated by the Yamanaka factors Oct4, Sox2, Klf4, and c-Myc. The resulting induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine by virtue of their ability to differentiate into different types of functional cells. Specifically, iPSCs derived directly from patients offer a powerful platform for creating in vitro disease models
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Navigating the Landscape of Intestinal Regeneration: A Spotlight on Quiescence Regulation and Fetal Reprogramming. Int. J. Stem Cells (IF 2.3) Pub Date : 2024-01-25 Su-Jeong Oh, Yoojin Seo, Hyung-Sik Kim
Tissue-specific adult stem cells are pivotal in maintaining tissue homeostasis, especially in the rapidly renewing intestinal epithelium. At the heart of this process are leucine-rich repeat-containing G protein-coupled receptor 5-expressing crypt base columnar cells (CBCs) that differentiate into various intestinal epithelial cells. However, while these CBCs are vital for tissue turnover, they are
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Human Pluripotent Stem Cell-Derived Retinal Organoids: A Viable Platform for Investigating the Efficacy of Adeno-Associated Virus Gene Therapy. Int. J. Stem Cells (IF 2.3) Pub Date : 2024-01-22 Hyeon-Jin Na, Jae-Eun Kwon, Seung-Hyun Kim, Jiwon Ahn, Ok-Seon Kwon, Kyung-Sook Chung
With recent advances in adeno-associated virus (AAV)-based gene therapy, efficacy and toxicity screening have become essential for developing gene therapeutic drugs for retinal diseases. Retinal organoids from human pluripotent stem cells (hPSCs) offer a more accessible and reproducible human test platform for evaluating AAV-based gene therapy. In this study, hPSCs were differentiated into retinal
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Exosomes Reshape the Osteoarthritic Defect: Emerging Potential in Regenerative Medicine-A Review. Int. J. Stem Cells (IF 2.3) Pub Date : 2024-01-22 Jaishree Sankaranarayanan, Seok Cheol Lee, Hyung Keun Kim, Ju Yeon Kang, Sree Samanvitha Kuppa, Jong Keun Seon
Osteoarthritis (OA) is a joint disorder caused by wear and tear of the cartilage that cushions the joints. It is a progressive condition that can cause significant pain and disability. Currently, there is no cure for OA, though there are treatments available to manage symptoms and slow the progression of the disease. A chondral defect is a common and devastating lesion that is challenging to treat
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Cytoplasmatic Localization of Six1 in Male Testis and Spermatogonial Stem Cells. Int. J. Stem Cells (IF 2.3) Pub Date : 2024-01-16 Mingming Qin, Linzi Ma, Wenjing Du, Dingyao Chen, Guoqun Luo, Zhaoting Liu
Sine oculis homeobox 1 (Six1) is an important factor for embryonic development and carcinoma malignancy. However, the localization of Six1 varies due to protein size and cell types in different organs. In this study, we focus on the expression and localization of Six1 in male reproductive organ via bioinformatics analysis and immunofluorescent detection. The potential interacted proteins with Six1
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Host-Microbe Interactions Regulate Intestinal Stem Cells and Tissue Turnover in Drosophila. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-12-21 Ji-Hoon Lee
With the activity of intestinal stem cells and continuous turnover, the gut epithelium is one of the most dynamic tissues in animals. Due to its simple yet conserved tissue structure and enteric cell composition as well as advanced genetic and histologic techniques, Drosophila serves as a valuable model system for investigating the regulation of intestinal stem cells. The Drosophila gut epithelium
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Corrigendum to "Cyclic Phytosphingosine-1-Phosphate Primed Mesenchymal Stem Cells Ameliorate LPS-Induced Acute Lung Injury in Mice". Int. J. Stem Cells (IF 2.3) Pub Date : 2023-11-30 Youngheon Park,Jimin Jang,Jooyeon Lee,Hyosin Baek,Jaehyun Park,Sang-Ryul Cha,Se Bi Lee,Sunghun Na,Jae-Woo Kwon,Young Jun Park,Myeong Jun Choi,Kye-Seong Kim,Seok-Ho Hong,Se-Ran Yang
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Endothelial Progenitor Cells: A Brief Update. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-11-30 Amna Rashid Tariq, Mijung Lee, Manho Kim
An enormous amount of current data has suggested involvement of endothelial progenitor cells (EPCs) in neovasculogenesis in both human and animal models. EPC level is an indicator of possible cardiovascular risk such as Alzheimer disease. EPC therapeutics requires its identification, isolation, differentiation and thus expansion. We approach here the peculiar techniques through current and previous
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The Role of Exosomes from Mesenchymal Stem Cells in Spinal Cord Injury: A Systematic Review. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-11-29 Haoyu Wang,Chunxia Zhao,Qingqing Rong,Jinghe Cao,Hongyi Chen,Ruolin Li,Bin Zhang,Peng Xu
Spinal cord injury (SCI) is a serious nervous system disease that usually leads to the impairment of the motor, sensory, and autonomic nervous functions of the spinal cord, and it places a heavy burden on families and healthcare systems every year. Due to the complex pathophysiological mechanism of SCI and the poor ability of neurons to regenerate, the current treatment scheme has very limited effects
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Mimicking the Human Articular Joint with In Vitro Model of Neurons-Synoviocytes Co-Culture. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-11-24 Jakub Chwastek, Marta Kędziora, Małgorzata Borczyk, Michał Korostyński, Katarzyna Starowicz
The development of in vitro models is essential in modern science due to the need for experiments using human material and the reduction in the number of laboratory animals. The complexity of the interactions that occur in living organisms requires improvements in the monolayer cultures. In the work presented here, neuroepithelial stem (NES) cells were differentiated into peripheral-like neurons (PLN)
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Recent Research Trends in Stem Cells Using CRISPR/Cas-Based Genome Editing Methods. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-10-31 Da Eun Yoon,Hyunji Lee,Kyoungmi Kim
The clustered regularly interspaced short palindromic repeats (CRISPR) system, a rapidly advancing genome editing technology, allows DNA alterations into the genome of organisms. Gene editing using the CRISPR system enables more precise and diverse editing, such as single nucleotide conversion, precise knock-in of target sequences or genes, chromosomal rearrangement, or gene disruption by simple cutting
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Exploring the Molecular and Developmental Dynamics of Endothelial Cell Differentiation. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-10-26 Yu Jung Shin,Jung Hyun Lee
The development and differentiation of endothelial cells (ECs) are fundamental processes with significant implications for both health and disease. ECs, which are found in all organs and blood vessels, play a crucial role in facilitating nutrient and waste exchange and maintaining proper vessel function. Understanding the intricate signaling pathways involved in EC development holds great promise for
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Expression of Major Histocompatibility Complex during Neuronal Differentiation of Somatic Cell Nuclear Transfer-Human Embryonic Stem Cells. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-10-26 Jin Saem Lee,Jeoung Eun Lee,Shin-Hye Yu,Taehoon Chun,Mi-Yoon Chang,Dong Ryul Lee,Chang-Hwan Park
Human pluripotent stem cells (hPSCs) such as human embryonic stem cells (hESCs), induced pluripotent stem cells, and somatic cell nuclear transfer (SCNT)-hESCs can permanently self-renew while maintaining their capacity to differentiate into any type of somatic cells, thereby serving as an important cell source for cell therapy. However, there are persistent challenges in the application of hPSCs in
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Integrin α4 Positive Subpopulation in Adipose Derived Stem Cells Effectively Reduces Infarct Size through Enhanced Engraftment into Myocardial Infarction. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-10-19 Zihui Yuan, Juan Tan, Jian Wang
The efficacy of adipose-derived stem cells (ASCs) on myocardial infarction is limited due to poor survival and engraftment. Integrin-mediated cell adhesion is a prerequisite for its survival and homing. ASCs expressed insufficient integrin α4, limiting their homing capacity. This study aims to characterize integrin α4+ ASC subpopulation and investigate their therapeutic efficacy in myocardial infarction
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Nervonic Acid Inhibits Replicative Senescence of Human Wharton's Jelly-Derived Mesenchymal Stem Cells. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-10-12 Sun Jeong Kim, Soojin Kwon, Soobeen Chung, Eun Joo Lee, Sang Eon Park, Suk-Joo Choi, Soo-Young Oh, Gyu Ha Ryu, Hong Bae Jeon, Jong Wook Chang
Cellular senescence causes cell cycle arrest and promotes permanent cessation of proliferation. Since the senescence of mesenchymal stem cells (MSCs) reduces proliferation and multipotency and increases immunogenicity, aged MSCs are not suitable for cell therapy. Therefore, it is important to inhibit cellular senescence in MSCs. It has recently been reported that metabolites can control aging diseases
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Lung Organoid on a Chip: A New Ensemble Model for Preclinical Studies. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-10-11 Hyung-Jun Kim, Sohyun Park, Seonghyeon Jeong, Jihoon Kim, Young-Jae Cho
The lung is a complex organ comprising a branched airway that connects the large airway and millions of terminal gas-exchange units. Traditional pulmonary biomedical research by using cell line model system have limitations such as lack of cellular heterogeneity, animal models also have limitations including ethical concern, race-to-race variations, and physiological differences found in vivo. Organoids
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Differentiation and Characterization of Cystic Fibrosis Transmembrane Conductance Regulator Knockout Human Pluripotent Stem Cells into Salivary Gland Epithelial Progenitors. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-09-06 Shuang Yan, Yifei Zhang, Siqi Zhang, Shicheng Wei
The differentiation of pluripotent stem cells has been used to study disease mechanisms and development. We previously described a method for differentiating human pluripotent stem cells (hPSCs) into salivary gland epithelial progenitors (SGEPs). Here, cystic fibrosis transmembrane conductance regulator (CFTR) knockout hPSCs were differentiated into SGEPs derived from CFTR knockout hESCs (CF-SGEPs)
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RNF43 and ZNRF3 in Wnt Signaling - A Master Regulator at the Membrane. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-08-30 Fiona Farnhammer, Gabriele Colozza, Jihoon Kim
The Wnt β-catenin signaling pathway is a highly conserved mechanism that plays a critical role from embryonic development and adult stem cell homeostasis. However, dysregulation of the Wnt pathway has been implicated in various diseases, including cancer. Therefore, multiple layers of regulatory mechanisms tightly control the activation and suppression of the Wnt signal. The E3 ubiquitin ligases RNF43
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Transcriptional Signature of Valproic Acid-Induced Neural Tube Defects in Human Spinal Cord Organoids. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-08-30 Ju-Hyun Lee, Mohammed R Shaker, Si-Hyung Park, Woong Sun
In vertebrates, the entire central nervous system is derived from the neural tube, which is formed through a conserved early developmental morphogenetic process called neurulation. Although the perturbations in neurulation caused by genetic or environmental factors lead to neural tube defects (NTDs), the most common congenital malformation and the precise molecular pathological cascades mediating NTDs
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In Vivo Stem Cell Imaging Principles and Applications. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-08-30 Seongje Hong, Dong-Sung Lee, Geun-Woo Bae, Juhyeong Jeon, Hak Kyun Kim, Siyeon Rhee, Kyung Oh Jung
Stem cells are the foundational cells for every organ and tissue in our body. Cell-based therapeutics using stem cells in regenerative medicine have received attracting attention as a possible treatment for various diseases caused by congenital defects. Stem cells such as induced pluripotent stem cells (iPSCs) as well as embryonic stem cells (ESCs), mesenchymal stem cells (MSCs), and neuroprogenitors
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Preclinical Study on Biodistribution of Mesenchymal Stem Cells after Local Transplantation into the Brain. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-08-30 Narayan Bashyal, Min Gyeong Kim, Jin-Hwa Jung, Rakshya Acharya, Young Jun Lee, Woo Sup Hwang, Jung-Mi Choi, Da-Young Chang, Sung-Soo Kim, Haeyoung Suh-Kim
Therapeutic efficacy of mesenchymal stem cells (MSCs) is determined by biodistribution and engraftment in vivo. Compared to intravenous infusion, biodistribution of locally transplanted MSCs are partially understood. Here, we performed a pharmacokinetics (PK) study of MSCs after local transplantation. We grafted human MSCs into the brains of immune-compromised nude mice. Then we extracted genomic DNA
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Adipose Tissue-Derived Mesenchymal Stromal Cells from Ex-Morbidly Obese Individuals Instruct Macrophages towards a M2-Like Profile In Vitro. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-08-30 Daiana V Lopes Alves, Cesar Claudio-da-Silva, Marcelo C A Souza, Rosa T Pinho, Wellington Seguins da Silva, Periela S Sousa-Vasconcelos, Radovan Borojevic, Carmen M Nogueira, Hélio Dos S Dutra, Christina M Takiya, Danielle C Bonfim, Maria Isabel D Rossi
Obesity, which continues to increase worldwide, was shown to irreversibly impair the differentiation potential and angiogenic properties of adipose tissue mesenchymal stromal cells (ADSCs). Because these cells are intended for regenerative medicine, especially for the treatment of inflammatory conditions, and the effects of obesity on the immunomodulatory properties of ADSCs are not yet clear, here
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Extracellular Vesicles Derived from Mesenchymal Stem Cells as Cell-Free Therapy for Intrauterine Adhesion. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-06-30 Chao Li, Yuanjing Hu
Intrauterine adhesion (IUA) can occur after trauma to the basal layer of the endometrium, contributing to severe complications in females, such as infertility and amenorrhea. To date, the proposed therapeutic strategies are targeted to relieve IUA, such as hysteroscopic adhesiolysis, Foley catheter balloon, and hyaluronic acid injection have been applied in the clinic. However, these approaches showed
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Parathyroid Hormone-Related Protein Promotes the Proliferation of Patient-Derived Glioblastoma Stem Cells via Activating cAMP/PKA Signaling Pathway. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-06-30 Zhenyu Guo, Tingqin Huang, Yingfei Liu, Chongxiao Liu
Glioblastoma (GBM) is an aggressive primary brain tumor characterized by its heterogeneity and high recurrence and lethality rates. Glioblastoma stem cells (GSCs) play a crucial role in therapy resistance and tumor recurrence. Therefore, targeting GSCs is a key objective in developing effective treatments for GBM. The role of Parathyroid hormone-related peptide (PTHrP) in GBM and its impact on GSCs
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Applications of Bioinspired Platforms for Enhancing Immunomodulatory Function of Mesenchymal Stromal Cells. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-06-30 Ok-Hyeon Kim, Tae Jin Jeon, Young In So, Yong Kyoo Shin, Hyun Jung Lee
Mesenchymal stromal cells (MSCs) have attracted scientific and medical interest due to their self-renewing properties, pluripotency, and paracrine function. However, one of the main limitations to the clinical application of MSCs is their loss of efficacy after transplantation in vivo. Various bioengineering technologies to provide stem cell niche-like conditions have the potential to overcome this
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Peripheral Neuron-Organoid Interaction Induces Colonic Epithelial Differentiation via Non-Synaptic Substance P Secretion. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-06-30 Young Hyun Che, In Young Choi, Chan Eui Song, Chulsoon Park, Seung Kwon Lim, Jeong Hee Kim, Su Haeng Sung, Jae Hoon Park, Sun Lee, Yong Jun Kim
The colonic epithelial layer is a complex structure consisting of multiple cell types that regulate various aspects of colonic physiology, yet the mechanisms underlying epithelial cell differentiation during development remain unclear. Organoids have emerged as a promising model for investigating organogenesis, but achieving organ-like cell configurations within colonic organoids is challenging. Here
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Low-Intensity Pulsed Ultrasound Promotes BMP9 Induced Osteoblastic Differentiation in Rat Dedifferentiated Fat Cells. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-06-30 Fumiaki Setoguchi, Kotaro Sena, Kazuyuki Noguchi
Dedifferentiated fat cells (DFATs) isolated from mature adipocytes have a multilineage differentiation capacity similar to mesenchymal stem cells and are considered as promising source of cells for tissue engineering. Bone morphogenetic protein 9 (BMP9) and low-intensity pulsed ultrasound (LIPUS) have been reported to stimulate bone formation both in vitro and in vivo. However, the combined effect
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Measuring Glutathione Regeneration Capacity in Stem Cells. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-06-30 Jihye Kim, Yi-Xi Gong, Eui Man Jeong
Glutathione (GSH) is a chief cellular antioxidant, affecting stem cell functions. The cellular GSH level is dynamically altered by the redox buffering system and transcription factors, including NRF2. Additionally, GSH is differentially regulated in each organelle. We previously reported a protocol for monitoring the real-time GSH levels in live stem cells using the reversible GSH sensor FreSHtracer
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Assessment of Risks and Benefits of Using Antibiotics Resistance Genes in Mesenchymal Stem Cell-Based Ex-vivo Therapy. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-06-30 Narayan Bashyal, Young Jun Lee, Jin-Hwa Jung, Min Gyeong Kim, Kwang-Wook Lee, Woo Sup Hwang, Sung-Soo Kim, Da-Young Chang, Haeyoung Suh-Kim
Recently, ex-vivo gene therapy has emerged as a promising approach to enhance the therapeutic potential of mesenchymal stem cells (MSCs) by introducing functional genes in vitro. Here, we explored the need of using selection markers to increase the gene delivery efficiency and evaluated the potential risks associated with their use in the manufacturing process.
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Notch is Not Involved in Physioxia-Mediated Stem Cell Maintenance in Midbrain Neural Stem Cells. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-04-30 Anne Herrmann,Anne K Meyer,Lena Braunschweig,Lisa Wagenfuehr,Franz Markert,Deborah Kolitsch,Vladimir Vukicevic,Christiane Hartmann,Marlen Siebert,Monika Ehrhart-Bornstein,Andreas Hermann,Alexander Storch
Background and Objectives The physiological oxygen tension in fetal brains (∼3%, physioxia) is beneficial for the maintenance of neural stem cells (NSCs). Sensitivity to oxygen varies between NSCs from different fetal brain regions, with midbrain NSCs showing selective susceptibility. Data on Hif-1α/Notch regulatory interactions as well as our observations that Hif-1α and oxygen affect midbrain NSCs
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Cyclic Phytosphingosine-1-Phosphate Primed Mesenchymal Stem Cells Ameliorate LPS-Induced Acute Lung Injury in Mice. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-04-30 Youngheon Park,Jimin Jang,Jooyeon Lee,Hyosin Baek,Jaehyun Park,Sang-Ryul Cha,Se Bi Lee,Sunghun Na,Jae-Woo Kwon,Seok-Ho Hong,Se-Ran Yang
Background and Objectives O-cyclic phytosphingosine-1-phosphate (cP1P) is a synthetic chemical and has a structure like sphingosine-1-phosphate (S1P). S1P is known to promote cell migration, invasion, proliferation, and anti-apoptosis through hippocampal signals. However, S1P mediated cellular-, molecular mechanism is still remained in the lung. Acute lung injury (ALI) and its severe form acute respiratory
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Dissection of Cellular Communication between Human Primary Osteoblasts and Bone Marrow Mesenchymal Stem Cells in Osteoarthritis at Single-Cell Resolution. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-04-30 Ying Liu,Yan Chen,Xiao-Hua Li,Chong Cao,Hui-Xi Zhang,Cui Zhou,Yu Chen,Yun Gong,Jun-Xiao Yang,Liang Cheng,Xiang-Ding Chen,Hui Shen,Hong-Mei Xiao,Li-Jun Tan,Hong-Wen Deng
Background and Objectives Osteoblasts are derived from bone marrow mesenchymal stem cells (BMMSCs) and play important role in bone remodeling. While our previous studies have investigated the cell subtypes and heterogeneity in osteoblasts and BMMSCs separately, cell-to-cell communications between osteoblasts and BMMSCs in vivo in humans have not been characterized. The aim of this study was to investigate
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Alterations and Co-Occurrence of C-MYC, N-MYC, and L-MYC Expression are Related to Clinical Outcomes in Various Cancers. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-04-30 Moonjung Lee,Jaekwon Seok,Subbroto Kumar Saha,Sungha Cho,Yeojin Jeong,Minchan Gil,Aram Kim,Ha Youn Shin,Hojae Bae,Jeong Tae Do,Young Bong Kim,Ssang-Goo Cho
Background and Objectives MYC, also known as an oncogenic reprogramming factor, is a multifunctional transcription factor that maintains induced pluripotent stem cells (iPSCs). Although MYC is frequently upregulated in various cancers and is correlated with a poor prognosis, MYC is downregulated and correlated with a good prognosis in lung adenocarcinoma. MYC and two other MYC family genes, MYCN and
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Hypoxia Differentially Affects Chondrogenic Differentiation of Progenitor Cells from Different Origins. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-04-30 Mira Hammad,Alexis Veyssiere,Sylvain Leclercq,Vincent Patron,Catherine Baugé,Karim Boumédiene
Background and Objectives Ear cartilage malformations are commonly encountered problems in reconstructive surgery, since cartilage has low self-regenerating capacity. Malformations that impose psychological and social burden on one's life are currently treated using ear prosthesis, synthetic implants or autologous flaps from rib cartilage. These approaches are challenging because not only they request
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Effect of Xenogeneic Substances on the Glycan Profiles and Electrophysiological Properties of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-04-30 Yong Guk Kim,Jun Ho Yun,Ji Won Park,Dabin Seong,Su-Hae Lee,Ki Dae Park,Hyang-Ae Lee,Misun Park
Background and Objectives Human induced pluripotent stem cell (hiPSC)-derived cardiomyocyte (CM) hold great promise as a cellular source of CM for cardiac function restoration in ischemic heart disease. However, the use of animal-derived xenogeneic substances during the biomanufacturing of hiPSC-CM can induce inadvertent immune responses or chronic inflammation, followed by tumorigenicity. In this
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Transition Substitution of Desired Bases in Human Pluripotent Stem Cells with Base Editors: A Step-by-Step Guide. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-02-28 Ju-Chan Park, Keun-Tae Kim, Hyeon-Ki Jang, Hyuk-Jin Cha
The recent advances in human pluripotent stem cells (hPSCs) enable to precisely edit the desired bases in hPSCs to be used for the establishment of isogenic disease models and autologous ex vivo cell therapy. The knock-in approach based on the homologous directed repair with Cas9 endonuclease, causing DNA double-strand breaks (DSBs), produces not only insertion and deletion (indel) mutations but also
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Strategic Application of Epigenetic Regulators for Efficient Neuronal Reprogramming of Human Fibroblasts. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-02-28 Gary Stanley Fernandes, Rishabh Deo Singh, Debojyoti De, Kyeong Kyu Kim
Cellular reprogramming in regenerative medicine holds great promise for treating patients with neurological disorders. In this regard, small molecule-mediated cellular conversion has attracted special attention because of its ease of reproducibility, applicability, and fewer safety concerns. However, currently available protocols for the direct conversion of somatic cells to neurons are limited in
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The Essential Function of miR-5739 in Embryonic Muscle Development. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-02-28 Ji-Heon Lee, Min Sup Kim, Jin-Seop Lee, Dong Hyun Lee, Chansol Park, Dong Hyuk Lee, Eun-Young Kim, Hyung Min Chung
Embryologically, mesodermal development is closely related to the development of various organs such as muscles, blood vessels, and hearts, which are the main organs that make up the body. However, treatment for mesoderm developmental disorders caused by congenital or acquired factors has so far relied on surgery and drug treatment for symptom relief, and more fundamentally, treatment for mesoderm
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The Suppression Effects of Fat Mass and Obesity Associated Gene on the Hair Follicle-Derived Neural Crest Stem Cells Differentiating into Melanocyte by N6-Methyladenosine Modifying Microphthalmia-Associated Transcription Factor. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-02-28 Zhiwei Shang, Haixia Feng, Liye Xia
Melanocyte (MC), derived from neural crest stem cell (NCSC), are involved in the production of melanin. The mechanism by which NCSC differentiates to MC remains unclear. N6-methyladenosine (m6A) modification was applied to discuss the potential mechanism.
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The Role of SDF-1α-CXCR4/CXCR7 in Migration of Human Periodontal Ligament Stem Cells. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-02-28 Jialei Xu, Fan Yang, Shuhan Luo, Yuan Gao, Dingming Huang, Lan Zhang
Regenerative endodontic procedures (REPs) are a research hotspot in the endodontic field. One of the biggest problems of REPs is that it is difficult to realize regeneration of pulp-dentin complex and functional reconstruction. The reason is still not clear. We hypothesize that the migration may be different in different dental stem cells. Periodontal ligament stem cells (PDLSCs) may migrate faster
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Exosomes from Tension Force-Applied Periodontal Ligament Cells Promote Mesenchymal Stem Cell Recruitment by Altering microRNA Profiles. Int. J. Stem Cells (IF 2.3) Pub Date : 2023-02-28 Maolin Chang, Qianrou Chen, Beike Wang, Zhen Zhang, Guangli Han
To investigate the role of exosomes from periodontal ligament cells (PDLCs) in bone marrow mesenchymal stem cell (BMSC) migration.
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Regenerative Effect of Adipose Derived Mesenchymal Stem Cells on Ganglion Cells in the Hypoxic Organotypic Retina Culture. Int. J. Stem Cells (IF 2.3) Pub Date : 2022-12-31 Meital Ben Dov,Bryan Krief,Moshe Benhamou,Ainat Klein,Shula Schwartz,Anat Loewenstein,Adiel Barak,Aya Barzelay
Background and Objectives To examine whether ischemic retinal ganglion cells (RGCs) will be salvaged from cell death by human adipose-derived mesenchymal stem cells (ADSCs) in an organotypic retina model. Methods and Results Deprived of arterial oxygen supply, whole mice retinas were cultured as an ex vivo organotypic cultures on an insert membrane in a 24-well plate. The therapeutic potential of ADSCs
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The Calcineurin-Drp1-Mediated Mitochondrial Fragmentation Is Aligned with the Differentiation of c-Kit Cardiac Progenitor Cells. Int. J. Stem Cells (IF 2.3) Pub Date : 2022-12-31 Attaur Rahman,Yuhao Li,Nur Izzah Ismail,To-Kiu Chan,Yuzhen Li,Dachun Xu,Hao Zhou,Sang-Bing Ong
Objective The heart contains a pool of c-kit+ progenitor cells which is believed to be able to regenerate. The differentiation of these progenitor cells is reliant on different physiological cues. Unraveling the underlying signals to direct differentiation of progenitor cells will be beneficial in controlling progenitor cell fate. In this regard, the role of the mitochondria in mediating cardiac progenitor
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Generation of Induced Pluripotent Stem Cells from Lymphoblastoid Cell Lines by Electroporation of Episomal Vectors. Int. J. Stem Cells (IF 2.3) Pub Date : 2022-12-31 Myunghyun Kim,Junmyeong Park,Sujin Kim,Dong Wook Han,Borami Shin,Hans Robert Schöler,Johnny Kim,Kee-Pyo Kim
Background and Objectives Lymphoblastoid cell lines (LCLs) deposited from disease-affected individuals could be a valuable donor cell source for generating disease-specific induced pluripotent stem cells (iPSCs). However, generation of iPSCs from the LCLs is still challenging, as yet no effective gene delivery strategy has been developed. Methods and Results Here, we reveal an effective gene delivery
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Reduced Cytotoxicity by Repetitive mRNA Transfection in Differentiated Neurons. Int. J. Stem Cells (IF 2.3) Pub Date : 2022-12-31 Seung Hwan Ko,Jin Sun Kang,Sang-Mi Kim,Eun-Hye Lee,Chang-Hwan Park
Background and Objectives mRNA-based protein expression technology has been used to express functional proteins. We have previously generated dopamine neurons from rat-embryo derived neural precursor cells (NPCs) through repeated transfection of synthetic transcription factor mRNA encoding dopamine-inducible genes. However, NPCs began to die approximately 10 d post-transfection. In this study, we examined
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Inhibition of Class I Histone Deacetylase Enhances Self-Reprogramming of Spermatogonial Stem Cells into Pluripotent Stem Cells. Int. J. Stem Cells (IF 2.3) Pub Date : 2022-12-31 Yukyeong Lee,Seung-Won Lee,Dahee Jeong,Hye Jeong Lee,Na Young Choi,Jin Seok Bang,Seokbeom Ham,Kinarm Ko
Background and Objectives Spermatogonial stem cells (SSCs) are the most primitive cells in spermatogenesis and are the only adult stem cells capable of passing on the genome of a given species to the next generation. SSCs are the only adult stem cells known to exhibit high Oct4 expression and can be induced to self-reprogram into pluripotent cells depending on culture conditions. Epigenetic modulation
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Establishing Rationale for the Clinical Development of Cell Therapy Products: Consensus between Risk and Benefit. Int. J. Stem Cells (IF 2.3) Pub Date : 2022-12-31 Seunghoon Han,Hyeon Woo Yim,Hyunsuk Jeong,Suein Choi,Sungpil Han
Despite long-term research achievements, the development of cell therapy (CT) products remains challenging. This is because the risks experienced by the subject and therapeutic effects in the clinical trial stage are unclear due to the various uncertainties of CT when administered to humans. Nevertheless, as autologous cell products for systemic administration have recently been approved for marketing
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Therapeutic Effect of Three-Dimensional Cultured Adipose-Derived Stem Cell-Conditioned Medium in Renal Ischemia-Reperfusion Injury. Int. J. Stem Cells (IF 2.3) Pub Date : 2022-10-31 Yu Seon Kim, Joomin Aum, Bo Hyun Kim, Myoung Jin Jang, Jungyo Suh, Nayoung Suh, Dalsan You
We evaluated the effect of adipose-derived stem cell-derived conditioned medium (ADSC-CM) on the renal function of rats with renal ischemia-reperfusion injury (IRI)-induced acute kidney injury.
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Lymphoid Lineage γδ T Cells Were Successfully Generated from Human Pluripotent Stem Cells via Hemogenic Endothelium. Int. J. Stem Cells (IF 2.3) Pub Date : 2022-10-31 Soo-Been Jeon, A-Reum Han, Yoo Bin Choi, Ah Reum Lee, Ji Yoon Lee
γδ T cells are a rare and unique prototype of T cells that share properties with natural killer cells in secondary lymphoid organs. Although many studies have revealed the function and importance of adult-derived γδ T cells in cancer biology and regenerative medicine, the low numbers of these cells hamper their application as therapeutic cell sources in the clinic. To solve this problem, pluripotent
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Embryonic Stem Cells Lacking DNA Methyltransferases Differentiate into Neural Stem Cells that Are Defective in Self-Renewal. Int. J. Stem Cells (IF 2.3) Pub Date : 2022-10-31 Bong Jong Seo, Tae Kyung Hong, Sang Hoon Yoon, Jae Hoon Song, Sang Jun Uhm, Hyuk Song, Kwonho Hong, Hans Robert Schöler, Jeong Tae Do
DNA methyltransferases (Dnmts) play an important role in regulating DNA methylation during early developmental processes and cellular differentiation. In this study, we aimed to investigate the role of Dnmts in neural differentiation of embryonic stem cells (ESCs) and in maintenance of the resulting neural stem cells (NSCs).
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In Vitro Generation of Luminal Vasculature in Liver Organoids: From Basic Vascular Biology to Vascularized Hepatic Organoids. Int. J. Stem Cells (IF 2.3) Pub Date : 2022-10-31 Hyo Jin Kim, Gyeongmin Kim, Kyun Yoo Chi, Jong-Hoon Kim
Liver organoids have gained much attention in recent years for their potential applications to liver disease modeling and pharmacologic drug screening. Liver organoids produced in vitro reflect some aspects of the in vivo physiological and pathological conditions of the liver. However, the generation of liver organoids with perfusable luminal vasculature remains a major challenge, hindering precise
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Transcriptomic Comparison Analysis between Ameloblastoma and AM-1 Cell Line. Int. J. Stem Cells (IF 2.3) Pub Date : 2022-10-31 Shujin Li, Dong-Joon Lee, Hyun-Yi Kim, Hidemitsu Harada, Young-Soo Jung, Han-Sung Jung
Cancer initiation and progression are profoundly along with the crosstalk between cancer cells and the surrounding stroma. Accumulating evidence has shown that the therapy targeting the extracellular matrix (ECM) would regress tumor growth and invasion in the most common carcinomas. However, it remains largely unexplored in several rare tumors like odontogenic tumors. Ameloblastoma (AM) is the representative
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Wedelolactone Promotes the Chondrogenic Differentiation of Mesenchymal Stem Cells by Suppressing EZH2. Int. J. Stem Cells (IF 2.3) Pub Date : 2022-10-31 Wei Qin, Lin Yang, Xiaotong Chen, Shanyu Ye, Aijun Liu, Dongfeng Chen, Kunhua Hu
Osteoarthritis (OA) is a degenerative disease that leads to the progressive destruction of articular cartilage. Current clinical therapeutic strategies are moderately effective at relieving OA-associated pain but cannot induce chondrocyte differentiation or achieve cartilage regeneration. We investigated the ability of wedelolactone, a biologically active natural product that occurs in Eclipta alba
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Synergistic Effect of Hydrogen and 5-Aza on Myogenic Differentiation through the p38 MAPK Signaling Pathway in Adipose-Derived Mesenchymal Stem Cells. Int. J. Stem Cells (IF 2.3) Pub Date : 2022-08-31 Wenyong Fei, Erkai Pang, Lei Hou, Jihang Dai, Mingsheng Liu, Xuanqi Wang, Bin Xie, Jingcheng Wang
This study aims to clarify the systems underlying regulation and regulatory roles of hydrogen combined with 5-Aza in the myogenic differentiation of adipose mesenchymal stem cells (ADSCs).
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Tracking of Stem Cells from Human Exfoliated Deciduous Teeth Labeled with Molday ION Rhodamine-B during Periodontal Bone Regeneration in Rats. Int. J. Stem Cells (IF 2.3) Pub Date : 2022-08-31 Nan Zhang, Li Xu, Hao Song, Chunqing Bu, Jie Kang, Chuanchen Zhang, Xiaofei Yang, Fabin Han
Chronic periodontitis can lead to alveolar bone resorption and eventually tooth loss. Stem cells from exfoliated deciduous teeth (SHED) are appropriate bone regeneration seed cells. To track the survival, migration, and differentiation of the transplanted SHED, we used super paramagnetic iron oxide particles (SPIO) Molday ION Rhodamine-B (MIRB) to label and monitor the transplanted cells while repairing
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Modulation of Osteogenic Differentiation of Adipose-Derived Stromal Cells by Co-Treatment with 3, 4'-Dihydroxyflavone, U0126, and N-Acetyl Cysteine. Int. J. Stem Cells (IF 2.3) Pub Date : 2022-06-30 Kwonwoo Song, Gwang-Mo Yang, Jihae Han, Minchan Gil, Ahmed Abdal Dayem, Kyeongseok Kim, Kyung Min Lim, Geun-Ho Kang, Sejong Kim, Soo Bin Jang, Balachandar Vellingiri, Ssang-Goo Cho
Flavonoids form the largest group of plant phenols and have various biological and pharmacological activities. In this study, we investigated the effect of a flavonoid, 3, 4'-dihydroxyflavone (3, 4'-DHF) on osteogenic differentiation of equine adipose-derived stromal cells (eADSCs).
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Promotes Endothelial Differentiation and Angiogenesis of Periodontal Ligament Stem Cells by Activating Piezo1. Int. J. Stem Cells (IF 2.3) Pub Date : 2022-06-30 Rui Hu, Zheng-Yan Yang, Yue-Heng Li, Zhi Zhou
Low-intensity pulsed ultrasound (LIPUS) promotes differentiation and regulates biological functions of various stem cells, but its effect on the endothelial differentiation of periodontal ligament stem cells (PDLSCs) is unclear. This study investigated the effect of LIPUS on endothelial differentiation and angiogenesis in PDLSCs and the role of the mechanically sensitive ion channel Piezo1 in this
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Generation of Urothelial Cells from Mouse-Induced Pluripotent Stem Cells. Int. J. Stem Cells (IF 2.3) Pub Date : 2022-06-30 Dongxu Zhang, Fengze Sun, Huibao Yao, Di Wang, Xingjun Bao, Jipeng Wang, Jitao Wu
The search for a suitable alternative for urethral defect is a challenge in the field of urethral tissue engineering. Induced pluripotent stem cells (iPSCs) possess multipotential for differentiation. The in vitro derivation of urothelial cells from mouse-iPSCs (miPSCs) has thus far not been reported. The purpose of this study was to establish an efficient and robust differentiation protocol for the
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NBCe1 Regulates Odontogenic Differentiation of Human Dental Pulp Stem Cells via NF-κB. Int. J. Stem Cells (IF 2.3) Pub Date : 2022-06-30 Qin Li, Yanqin Ju, Changlong Jin, Li Liu, Shouliang Zhao
Dental pulp stem cells (DPSCs) play an important role in the repair of tooth injuries. Electrogenic sodium bicarbonate cotransporter 1 (NBCe1) is a Na+-coupled HCO3- transporter encoded by the solute carrier 4A4 (SLC4A4) gene and plays a crucial role in maintaining the pH of DPSCs. Our previous research confirmed that NBCe1 is highly expressed in odontoblasts during the development of the tooth germ