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Sphingolipid diversity in Candida auris: unraveling interclade and drug resistance fingerprints FEMS Yeast Res. (IF 3.2) Pub Date : 2024-03-04 Basharat Ali, Mohit Kumar, Praveen Kumar, Anshu Chauhan, Sana Akhtar Usmani, Shivaprakash M Rudramurthy, Jacques F Meis, Arunaloke Chakrabarti, Ashutosh Singh, Naseem A Gaur, Alok K Mondal, Rajendra Prasad
In this study, we explored the sphingolipid (SL) landscape in Candida auris, which plays pivotal roles in fungal biology and drug susceptibility. The composition of SLs exhibited substantial variations at both the SL class and molecular species levels among clade isolates. Utilizing Principal Component Analysis, we successfully differentiated the five clades based on their SL class composition. While
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Engineering of Ogataea polymorpha strains with ability for high-temperature alcoholic fermentation of cellobiose FEMS Yeast Res. (IF 3.2) Pub Date : 2024-02-22 Roksolana Vasylyshyn, Olena Dmytruk, Andriy Sibirny, Justyna Ruchala
Successful conversion of cellulosic biomass into biofuels requires organisms capable of efficiently utilizing xylose as well as cellodextrins and glucose. Ogataea (Hansenula) polymorpha is the natural xylose-metabolizing organism and is one of the most thermotolerant yeasts known, with a maximum growth temperature above 50°C. Cellobiose-fermenting strains, derivatives of an improved ethanol producer
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Mutations of ribosomal protein genes induce overexpression of catalase in Saccharomyces cerevisiae FEMS Yeast Res. (IF 3.2) Pub Date : 2024-01-25 Ching-Hsiang Hsu, Ching-Yu Liu, L O Kai-Yin
Ribosome assembly defects result in ribosomopathies, primarily caused by inadequate protein synthesis and induced oxidative stress. This study aimed to investigate the link between deleting one ribosomal protein gene (RPG) paralog and oxidative stress response. Our results indicated that RPG mutants exhibited higher oxidant sensitivity than the wild type (WT). The concentrations of H2O2 were increased
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Construction of an economical Xylose-utilizing Saccharomyces cerevisiae and its ethanol fermentation FEMS Yeast Res. (IF 3.2) Pub Date : 2024-01-25 Fan Li, Wenxin Bai, Yuan Zhang, Zijian Zhang, Deguo Zhang, Naidong Shen, Jingwei Yuan, Guomiao Zhao, Xiaoyan Wang
Traditional industrial Saccharomyces cerevisiae could not metabolize xylose due to the lack of a specific enzyme system for the reaction from xylose to xylulose. This study aims to metabolically remould industrial S. cerevisiae for the purpose of utilizing both glucose and xylose with high efficiency. Heterologous gene xylA from Piromyces and homologous genes related to xylose utilization were selected
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Characterization of a novel 4-guanidinobutyrase from Candida parapsilosis FEMS Yeast Res. (IF 3.2) Pub Date : 2024-01-17 Santoshkumar R Gaikwad, Narayan S Punekar, Ejaj K Pathan
Enzymes of the ureohydrolase superfamily are specific in recognizing their substrates. While looking to broaden the substrate specificity of 4-guanidinobutyrase (GBase), we isolated a yeast, typed as Candida parapsilosis (NCIM 3689), that efficiently utilized both 4-guanidinobutyrate (GB) and 3-guanidinopropionate (GP) as a sole source of nitrogen. A putative GBase sequence was identified from its
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Natural trait variation across Saccharomycotina species FEMS Yeast Res. (IF 3.2) Pub Date : 2024-01-13 Johnson J-T Wang, Jacob L Steenwyk, Rachel B Brem
Among molecular biologists, the group of fungi called Saccharomycotina is famous for its yeasts. These yeasts in turn are famous for what they have in common—genetic, biochemical, and cell-biological characters that serve as models for plants and animals. But behind the apparent homogeneity of Saccharomycotina species lie a wealth of differences. In this review, we discuss traits that vary across the
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Application of unimodal probability distribution models for morphological phenotyping of budding yeast FEMS Yeast Res. (IF 3.2) Pub Date : 2024-01-02 Yoshikazu Ohya, Farzan Ghanegolmohammadi, Kaori Itto-Nakama
Morphological phenotyping of the budding yeast Saccharomyces cerevisiae has helped to greatly clarify the functions of genes and increase our understanding of cellular functional networks. It is necessary to understand cell morphology and perform quantitative morphological analysis (QMA) but assigning precise values to morphological phenotypes has been challenging. We recently developed the Unimodal
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The cell morphological diversity of Saccharomycotina yeasts FEMS Yeast Res. (IF 3.2) Pub Date : 2023-12-24 Christina M Chavez, Marizeth Groenewald, Amanda B Hulfachor, Gideon Kpurubu, Rene Huerta, Chris Todd Hittinger, Antonis Rokas
The ∼1 200 known species in subphylum Saccharomycotina are a highly diverse clade of unicellular fungi. During its lifecycle, a typical yeast exhibits multiple cell types with various morphologies; these morphologies vary across Saccharomycotina species. Here, we synthesize the evolutionary dimensions of variation in cellular morphology of yeasts across the subphylum, focusing on variation in cell
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Heterologous pulcherrimin production in Saccharomyces cerevisiae confers inhibitory activity on Botrytis conidiation FEMS Yeast Res. (IF 3.2) Pub Date : 2023-12-23 Florian M Freimoser, Marina Mahler, Mark McCullough, Alexander O Brachmann, Lukas Nägeli, Maja Hilber-Bodmer, Jörn Piel, Stefan Hoffmann, Yizhi Cai
Pulcherrimin is an iron (III) chelate of pulcherriminic acid that plays a role in antagonistic microbial interactions, iron metabolism and stress responses. Some bacteria and yeasts produce pulcherriminic acid, but so far, pulcherrimin could not be produced in Saccharomyces cerevisiae. Here, multiple integrations of the Metschnikowia pulcherrima PUL1 and PUL2 genes in the S. cerevisiae genome resulted
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Degradation of citrate synthase lacking the mitochondrial targeting sequence is inhibited in cells defective in Hsp70/Hsp40 chaperones under heat stress conditions FEMS Yeast Res. (IF 3.2) Pub Date : 2023-12-22 Mayuko Hayashi, Tomoyuki Kawarasaki, Kunio Nakatsukasa
Most nucleus-encoded mitochondrial precursor proteins are synthesized in the cytosol and imported into mitochondria in a post-translational manner. In recent years, the quality control mechanisms of non-imported mitochondrial proteins have been intensively studied. In a previous study, we established that in budding yeast a mutant form of citrate synthase 1 (N∆Cit1) that lacks the N-terminal mitochondrial
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Exploring the antioxidant activity of Fe(III), Mn(III)Mn(II), and Cu(II) compounds in Saccharomyces cerevisiae and Galleria mellonella models of study FEMS Yeast Res. (IF 3.2) Pub Date : 2023-12-21 Larissa M M Mattos, Hyan M Hottum, Daniele C Pires, Bruna B Segat, Adolfo Horn, Christiane Fernandes, Marcos D Pereira
Reactive oxygen species (ROS) are closely related to oxidative stress, aging, and the onset of human diseases. To mitigate ROS-induced damages, extensive research has focused on examining the antioxidative attributes of various synthetic/natural substances. Coordination compounds serving as synthetic antioxidants have emerged as a promising approach to attenuate ROS toxicity. Herein, we investigated
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QTL mapping reveals novel genes and mechanisms underlying variations in H2S production during alcoholic fermentation in Saccharomyces cerevisiae FEMS Yeast Res. (IF 3.2) Pub Date : 2023-12-16 Irene De Guidi, Céline Serre, Jessica Noble, Anne Ortiz-Julien, Bruno Blondin, Jean-Luc Legras
Saccharomyces cerevisiae requirement for reduced sulfur to synthesise methionine and cysteine during alcoholic fermentation, is mainly fulfilled through the sulfur assimilation pathway. S. cerevisiae reduces sulfate into sulfur dioxide (SO2) and sulfide (H2S), whose overproduction is a major issue in winemaking, due to its negative impact on wine aroma. The amount of H2S produced is highly strain-specific
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Promoter-proximal introns impact recombinant amylase expression in Saccharomyces cerevisiae FEMS Yeast Res. (IF 3.2) Pub Date : 2023-10-28 Kirstie S Schwerdtfeger, Marthinus W Myburgh, Willem H van Zyl, Marinda Viljoen-Bloom
Consolidated bioprocessing (CBP) of starch requires recombinant Saccharomyces cerevisiae strains that produce raw starch-degrading enzymes and ferment the resultant sugars to ethanol in a single step. In this study, the native S. cerevisiae COX4 and RPS25A promoter-proximal introns were evaluated for enhanced expression of amylase genes (ateA, temA or temG_Opt) under the control of an S. cerevisiae
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Metabolic reconstruction of the human pathogen Candida auris: using a cross-species approach for drug target prediction FEMS Yeast Res. (IF 3.2) Pub Date : 2023-10-17 Romeu Viana, Tiago Carreiro, Diogo Couceiro, Oscar Dias, Isabel Rocha, Miguel Cacho Teixeira
Candida auris is an emerging human pathogen, associated with antifungal drug resistance and hospital candidiasis outbreaks. In this work, we present iRV973, the first reconstructed Genome-scale metabolic model (GSMM) for C. auris. The model was manually curated and experimentally validated, being able to accurately predict the specific growth rate of C. auris and the utilization of several sole carbon
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Sustainable production of single-cell oil and protein from wastepaper hydrolysate: identification and optimization of a Rhodotorula mucilaginosa strain as a promising yeast FEMS Yeast Res. (IF 3.2) Pub Date : 2023-10-05 Amador Campos-Valdez, Manuel R Kirchmayr, Iliana Barrera Martínez, Leticia Casas Godoy
This study investigated the potential of wastepaper hydrolysate as a sustainable and low-cost carbon source for single-cell oil and protein production, attending to the growing need for alternative feedstocks and waste management strategies. Wastepaper, characterized by its high carbohydrate content, was subjected to enzymatic and chemo-enzymatic treatments for carbohydrate release. The chemo-enzymatic
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Non-Saccharomyces yeast probiotics: revealing relevance and potential FEMS Yeast Res. (IF 3.2) Pub Date : 2023-09-29 Silvia Cristina Vergara Alvarez, María José Leiva Alaniz, María Victoria Mestre Furlani, Fabio Vazquez, María Cristina Nally, Yolanda Paola Maturano
Non-Saccharomyces yeasts are unicellular eukaryotes that play important roles in diverse ecological niches. In recent decades, their physiological and morphological properties have been reevaluated and reassessed, demonstrating the enormous potential they possess in various fields of application. Non-Saccharomyces yeasts have gained relevance as probiotics, and in vitro and in vivo assays are very
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Comparing the hierarchy of inter- and intraspecies interactions with population dynamics of wine yeast cocultures FEMS Yeast Res. (IF 3.2) Pub Date : 2023-08-31 Eléonore Pourcelot, Cleo Conacher, Thérèse Marlin, Florian Bauer, Virginie Galeote, Thibault Nidelet
In winemaking, the development of new fermentation strategies, such as the use of mixed starter cultures with Saccharomyces cerevisiae (Sc) yeast and non-Saccharomyces (NS) species, requires a better understanding of how yeasts interact, especially at the beginning of fermentation. Despite the growing knowledge on interactions between Sc and NS, few data are available on the interactions between different
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Production of Rhizopus oryzae lipase using optimized Yarrowia lipolytica expression system FEMS Yeast Res. (IF 3.2) Pub Date : 2023-07-27 Lea Vidal, Zehui Dong, Kim Olofsson, Eva Nordberg Karlsson, Jean-Marc Nicaud
Yarrowia lipolytica is an alternative yeast for heterologous protein production. Based on auto-cloning vectors, a set of 18 chromogenic cloning vectors was developed, each containing one of the excisable auxotrophic selective markers URA3ex, LYS5ex and LEU2ex, and one of six different promoters: the constitutive pTEF, the phase dependent hybrid pHp4d, and the erythritol-inducible promoters from pEYK1
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PCR-based gene targeting in Hanseniaspora uvarum FEMS Yeast Res. (IF 3.2) Pub Date : 2023-07-26 Jennifer Badura, Niël van Wyk, Kerstin Zimmer, Isak S Pretorius, Christian von Wallbrunn, Jürgen Wendland
Lack of gene-function analyses tools limits studying the biology of Hanseniaspora uvarum, one of the most abundant yeasts on grapes and in must. We investigated a rapid PCR-based gene targeting approach for one-step gene replacement in this diploid yeast. To this end, we generated and validated two synthetic antibiotic resistance genes, pFA-hygXL and pFA-clnXL, providing resistance against hygromycin
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The genome sequence of the Champagne-Epernay-Geisenheim wine yeast reveals its hybrid nature FEMS Yeast Res. (IF 3.2) Pub Date : 2023-07-26 Beatrice Bernardi, Florian Michling, Judith Muno-Bender, Katrin Matti, Jürgen Wendland
Lager yeasts are hybrids between Saccharomyces cerevisiae and S. eubayanus. Wine yeast biodiversity, however, has only recently been discovered to include besides pure S. cerevisiae strains also hybrids between different Saccharomyces yeasts as well as introgressions from non-Saccharomyces species. Here we analysed the genome of the Champagne-Epernay-Geisenheim (CEG) wine yeast. This yeast is an allotetraploid
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CRISPR-Cas9 engineering in the hybrid yeast zygosaccharomyces parabailii can lead to loss of heterozygosity in target chromosomes FEMS Yeast Res. (IF 3.2) Pub Date : 2023-07-17 Pooja Jayaprakash, Liliane Barroso, Matteo Vajente, Letizia Maestroni, Edward J Louis, John P Morrissey, Paola Branduardi
The hybrid yeast Zygosaccharomyces parabailii holds potential as a cell factory mainly because of its robustness in withstanding stressors that often characterize bio-based processes. However, a complex genome and a lack of gene editing tools hinder the capacity to engineer this yeast. In this work, we developed a CRISPR-Cas9 gene editing system for Z. parabailii that allows simultaneous disruption
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My Journey with Yeast FEMS Yeast Res. (IF 3.2) Pub Date : 2023-06-23 Charles Abbas
Advances in yeast biotechnology rely on the application of knowledge gained using modern biotechnological tools to harness the metabolic repertoire of various yeast genera that have been studied in detail. In my work, I have attempted to combine knowledge gained from academic research with industrial knowhow in practical cost-effective ways to scale up commercial yeast fermentations from one hundred
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Polymorphisms of rDNA genes in Cyberlindnera yeast suggest birth and death evolution events FEMS Yeast Res. (IF 3.2) Pub Date : 2023-06-09 Rodolfo Bizarria, Tatiane de Castro Pietrobon, Henrique Ferreira, Andre Rodrigues
In eukaryotes, the ribosome machinery is encoded by repeats of the ribosomal RNA genes: 26/28S, 18S, 5.8S, and 5S, structured in tandem arrays and frequently homogenized within a genome. This homogenization is thought to be driven by concerted evolution, evolving as a unit, which contributes to its target as the species barcode in modern taxonomy. However, high heterogeneity of rDNA genes has been
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Hydrogen sulfide production during early yeast fermentation correlates with volatile sulfur compound biogenesis but not thiol release FEMS Yeast Res. (IF 3.2) Pub Date : 2023-06-09 Ruoyu Hou, Rebecca E Jelley, Katryna A van Leeuwen, Farhana R Pinu, Bruno Fedrizzi, Rebecca C Deed
Yeasts undergo intensive metabolic changes during the early stages of fermentation. Previous reports suggest the early production of hydrogen sulfide (H2S) is associated with the release of a range of volatile sulfur compounds (VSCs), as well as the production of varietal thiol compounds 3-sulfanylhexan-1-ol (3SH) and 3-sulfanylhexyl acetate (3SHA) from six-carbon precursors including (E)-hex-2-enal
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Rice-based fermented products: The functional properties of the microorganisms in the defined starter contributing to melanogenesis inhibition activity FEMS Yeast Res. (IF 3.2) Pub Date : 2023-05-28 Orrarat Sangkaew, Chulee Yompakdee
Rice contains numerous nutrients and biologically active compounds. The phytochemical composition of rice varies among cultivars that leads to diversities in biological activities. Fermentation is an efficient way for improving nutrient bioavailability and functional properties of raw materials. It enhances and/or synthesizes the compounds with health promoting or decreased antinutritive compounds
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Malassezia sympodialis Mala s 1 allergen is a potential KELCH protein that cross reacts with human skin FEMS Yeast Res. (IF 3.2) Pub Date : 2023-05-16 Dora E Corzo Leon, Annika Scheynius, Donna M MacCallum, Carol A Munro
Malassezia are the dominant commensal yeast species of the human skin microbiota and are associated with inflammatory skin diseases, such as atopic eczema (AE). The Mala s 1 allergen of Malassezia sympodialis is a β-propeller protein, inducing both IgE and T-cell reactivity in AE patients. We demonstrate by immuno-electron microscopy that Mala s 1 is mainly located in the M. sympodialis yeast cell
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Using the AKAR3-EV biosensor to assess Sch9p- & PKA-signalling in budding yeast FEMS Yeast Res. (IF 3.2) Pub Date : 2023-05-13 Dennis Botman, Sineka Kanagasabapathi, Philipp Savakis, Bas Teusink
Budding yeast uses the TORC1-Sch9p and cAMP-PKA signalling pathways to regulate adaptations to changing nutrient environments. Dynamic and single-cell measurements of the activity of these cascades will improve our understanding of cellular adaptation of yeast. Here, we employed the AKAR3-EV biosensor developed for mammalian cells to measure the cellular phosphorylation status determined by Sch9p and
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How do engineered Yarrowia lipolytica strains secrete free fatty acids: hints from comparative transcriptomics FEMS Yeast Res. (IF 3.2) Pub Date : 2023-05-08 José Manuel Salvador López, Lea Vidal, Michelle Patricia Adiutama, Filip Van Nieuwerburgh, Dieter Deforce, Jean-Marc Nicaud, Inge Noëlle Adrienne Van Bogaert
Yarrowia lipolytica has been considered one of the most promising platforms for the microbial production of fatty acids and derived products. The deletion of the faa1 gene coding for an acyl-CoA synthetase leads to the accumulation and secretion of free fatty acids (FFAs) into the extracellular space. The secretion of products is beneficial for the development of microbial cell factories to avoid intracellular
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Production of single cell oil by two novel nonconventional yeast strains of Curvibasidium sp. isolated from medicinal lichen FEMS Yeast Res. (IF 3.2) Pub Date : 2023-04-26 Long Bai, Cheng Cheng, Meng-Lin Sun, Jun Li, Yue Zou, Quanyu Zhao, Xin-Qing Zhao
Oleaginous yeasts utilize renewable resources to produce lipids, which benefits sustainable development, and it is of great interest to screen robust lipid producers. Curvibasidium sp. belongs to nonconventional yeast that are very limitedly studied. Here, two cold-adaptive strains of Curvibasidium sp., namely, Y230 and Y231, isolated from the medicinal lichen Usnea diffracta were investigated for
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Travels with Metschnikowia FEMS Yeast Res. (IF 3.2) Pub Date : 2023-04-19 Marc-André Lachance
For over four decades, I have explored hotspots of biodiversity in search of yeasts that could increase our understanding of the meaning of species as the concept applies to yeasts. This led to the discovery, description, and characterization of many Metschnikowia and other species. What published species descriptions do not report is the context of their discoveries, the people and the places involved
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Finding a correct species assignment for a Metschnikowia strain: insights from the genome sequencing of strain DBT012 FEMS Yeast Res. (IF 3.2) Pub Date : 2023-04-06 Eleonora Troiano, Ilaria Larini, Renato L Binati, Veronica Gatto, Sandra Torriani, Pietro Buzzini, Benedetta Turchetti, Elisa Salvetti, Giovanna E Felis
Metschnikowia pulcherrima is an important yeast species that is attracting increased interest thanks to its biotechnological potential, especially in agri-food applications. Phylogenetically related species of the so-called ‘pulcherrima clade’ were first described and then reclassified in one single species, which makes the identification an intriguing issue. Starting from the whole-genome sequencing
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Yca1 metacaspase: Diverse functions determine how yeast live and let die FEMS Yeast Res. (IF 3.2) Pub Date : 2023-04-01 Darren K Lam, Gavin Sherlock
The Yca1 metacaspase was discovered due to its role in the regulation of apoptosis in Saccharomyces cerevisiae. However, the mechanisms that drive apoptosis in yeast remain poorly understood. Additionally, Yca1 and other metacaspase proteins have recently been recognized for their involvement in other cellular processes, including cellular proteostasis and cell cycle regulation. In this Minireview
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Insights into the transcriptional regulation of poorly characterized alcohol acetyltransferase-encoding genes (HgAATs) shed light into the production of acetate esters in the wine yeast Hanseniaspora guilliermondii. FEMS Yeast Res. (IF 3.2) Pub Date : 2023-01-04 Isabel Seixas,Diogo Santos,Isabel Vasconcelos,Nuno P Mira,Ana Mendes-Ferreira
Hanseniaspora guilliermondii is a well-recognized producer of acetate esters associated with fruity and floral aromas. The molecular mechanisms underneath this production or the environmental factors modulating it remain unknown. Herein, we found that, unlike Saccharomyces cerevisiae, H. guilliermondii over-produces acetate esters and higher alcohols at low carbon-to-assimilable nitrogen (C:N) ratios
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The essential role of spontaneous and starter yeasts in cocoa and coffee fermentation. FEMS Yeast Res. (IF 3.2) Pub Date : 2023-01-04 Rosane Freitas Schwan,Ana Paula Pereira Bressani,Silvia Juliana Martinez,Nadia Nara Batista,Disney Ribeiro Dias
Yeasts are important microorganisms used in different fermentation processes. The cocoa beans must go through a correct fermentation process to obtain good-quality chocolate, which involves the action of yeasts and bacteria, and yeasts play a crucial role since they act in the first days of fermentation. In coffee, several studies have shown that the microbiota in the fruits is also a relevant factor
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Development of vitamin B12 dependency in Saccharomyces cerevisiae FEMS Yeast Res. (IF 3.2) Pub Date : 2023-03-21 Sandra Lehner, Eckhard Boles
For decades the industrial vitamin B12 (cobalamin) production has been based on bacterial producer strains. Due to limited methods for strain optimization and difficult strain handling, the desire for new vitamin B12-producing hosts has risen. As a vitamin B12-independent organism with a big toolbox for genomic engineering and easy-to-handle cultivation conditions, Saccharomyces cerevisiae has high
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Metformin extends the chronological lifespan of fission yeast by altering energy metabolism and stress resistance capacity FEMS Yeast Res. (IF 3.2) Pub Date : 2023-03-21 Ceren Şeylan, Çağatay Tarhan
The anti-aging properties of metformin used for the treatment of type 2 diabetes mellitus have been studied extensively but there is more to discover regarding underlying mechanisms. Here we show that metformin significantly prolongs the chronological lifespan (CLS) of Schizosaccharomyces pombe through mechanisms similar to those observed in mammalian cells and other model organisms. While the presence
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The chromosomal evolutionary lineage of the genus Zygosaccharomyces FEMS Yeast Res. (IF 3.2) Pub Date : 2023-03-11 Atsushi Sato, Yasuo Ohnishi
Genome ploidy of Zygosaccharomyces rouxii is an intriguing topic in the field of industrial yeast research. However, the evolutionary relationship between the genome of Z. rouxii and other Zygosaccharomyces species is complex and not completely understood. In this study, we determined the genome sequences of Z. rouxii NCYC 3042, also referred to as “Z. pseudorouxii,” and Z. mellis CBS 736T. We also
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Split-marker-mediated genome editing improves homologous recombination frequency in the CTG clade yeast Candida intermedia FEMS Yeast Res. (IF 3.2) Pub Date : 2023-03-10 Kameshwara V R Peri, Fábio Faria-Oliveira, Adam Larsson, Alexander Plovie, Nicolas Papon, Cecilia Geijer
Genome editing toolboxes are essential for the exploration and exploitation of non-conventional yeast species as cell factories, as they facilitate both genome studies and metabolic engineering. The non-conventional yeast Candida intermedia is a biotechnologically interesting species due to its capacity to convert a wide range of carbon sources, including xylose and lactose found in forestry and dairy
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Current Trends and Future Perspective of Probiotic Yeasts Research in Indonesia FEMS Yeast Res. (IF 3.2) Pub Date : 2023-03-03 Rika Indri Astuti, Muhammad Eka Prastya, Rahayu Wulan, Khairul Anam, Anja Meryandini
Indonesia is a mega biodiversity country with various local wisdom, including the enormous variety of fermented foods and beverages. Indonesian researchers have conducted an intensive study to understand the diversity of microbes on those fermented products, one of which shows probiotic properties. Compared to that lactic acid bacteria, the study on probiotic yeasts is less explored. Probiotic yeast
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Metabolic engineering of saccharomyces cerevisiae for glycerol utilization FEMS Yeast Res. (IF 3.2) Pub Date : 2023-03-01 Ziqian Yu, Zhao Chang, Yinhua Lu, Han Xiao
Due to its wide availability, glycerol is considered as a promising alternative feedstock for microbial fermentation. As a model eukaryote, Saccharomyces cerevisiae is commonly adopted for bioproduction of various bulk and value-added chemicals, but it does not efficiently utilize glycerol. In this review, the metabolic pathway of glycerol and its regulation in S. cerevisiae are first introduced. Then
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Improvement of valine and isobutanol production in sake yeast by Ala31Thr substitution in the regulatory subunit of acetohydroxy acid synthase FEMS Yeast Res. (IF 3.2) Pub Date : 2023-02-23 Shota Isogai, Akira Nishimura, Naoyuki Murakami, Natsuki Hotta, Atsushi Kotaka, Yoichi Toyokawa, Hiroki Ishida, Hiroshi Takagi
The fruit-like aroma of two valine-derived volatiles, isobutanol and isobutyl acetate, has great impact on the flavor and taste of alcoholic beverages, including sake, a traditional Japanese alcoholic beverage. With the growing worldwide interest in sake, breeding of yeast strains with intracellular valine accumulation is a promising approach to meet a demand for sakes with a variety of flavor and
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Diversity of yeasts in Indian fermented foods and alcoholic beverages FEMS Yeast Res. (IF 3.2) Pub Date : 2023-02-21 Jyoti Prakash Tamang, Sonam Lama
Ethnic Indian people have been domesticating beneficial microorganisms (bacteria, yeasts and moulds) by their wisdom of ethno-microbiological knowledge for production of flavoured and socio-culturally preferred fermented foods and alcoholic beverages for more than 8000 years. The purpose of this review is to collate the available literatures of diversity of Saccharomyces and non- Saccharomyces species
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Biology and physiology of hanseniaspora vineae: metabolic diversity and increase flavour complexity for food fermentation FEMS Yeast Res. (IF 3.2) Pub Date : 2023-02-10 Francisco Carrau, Eduardo Dellacassa, Eduardo Boido, Karina Medina, Maria Jose Valera, Laura Fariña, Gabriel Perez, Valentina Martin, Fernando Alvarez-Valin, Lucia Balestrazzi
Apiculate yeasts belonging to the genus Hanseniaspora are predominant on grapes and other fruits. While some species, such as Hanseniaspora uvarum, are well known for their abundant presence in fruits, they are generally characterized by their detrimental effect on fermentation quality because the excessive production of acetic acid. However, the species Hanseniaspora vineae is adapted to fermentation
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Wide distribution of D-xylose dehydrogenase in yeasts reveals a new element in the D-xylose metabolism for bioethanol production FEMS Yeast Res. (IF 3.2) Pub Date : 2023-02-02 Juliana P Galhardo, André P Piffer, Mateus B Fiamenghi, Guilherme Borelli, Duguay R M da Silva, Adrielle A Vasconcelos, Marcelo F Carazzolle, Gonçalo A G Pereira, Juliana José
D-xylose utilization by yeasts is an essential feature for improving second-generation ethanol production. However, industrial yeast strains are incapable of consuming D-xylose. Previous analyzes of D-xylose-consuming or fermenting yeast species reveal that the genomic features associated with this phenotype are complex and still not fully understood. Here we present a previously neglected yeast enzyme
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Importance and mechanisms of S-adenosylmethionine and folate accumulation in sake yeast FEMS Yeast Res. (IF 3.2) Pub Date : 2023-02-02 Muneyoshi Kanai, Masaki Mizunuma, Tsutomu Fujii, Haruyuki Iefuji
Sake yeasts have a range of brewing characteristics that are particularly beneficial for sake making including high ethanol fermentability, high proliferative capacity at low temperatures, lactic acid tolerance, and high ester productivity. On the other hand, sake yeasts also accumulate a diverse range of functional components. For example, significantly greater accumulation of S-adenosylmethionine
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Mixed yeast communities contribute to regionally distinct wine attributes FEMS Yeast Res. (IF 3.2) Pub Date : 2023-02-02 Diana Lynne Hawkins, Jess Ryder, Soon A Lee, Katie Parish-Virtue, Bruno Fedrizzi, Matthew R Goddard, Sarah J Knight
There is evidence that vineyard yeast communities are regionally differentiated, but the extent to which this contributes to wine regional distinctiveness is not yet clear. This study represents the first experimental test of the hypothesis that mixed yeast communities—comprising multiple, region-specific, isolates and species—contribute to regional wine attributes. Yeast isolates were sourced from
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Tuning the expression of the bacterial relBE toxin-antitoxin system in Saccharomyces cerevisiae allows characterizing the subsequent growth inhibition FEMS Yeast Res. (IF 3.2) Pub Date : 2023-01-30 Maëlle Duperray, Jean-Marie François, Jean-Pascal Capp
The bacterial toxin-antitoxin systems are each composed of a toxin, which severely inhibits bacterial cells growth, and a specific neutralizing antitoxin. Some toxin-antitoxin systems are functional when expressed in the yeast Saccharomyces cerevisiae. For instance, the expression of the relE toxin gene leads to a strong growth defect in yeast, whereas the expression of the relB antitoxin gene restores
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Engineering a fermenting yeast able to produce the fragrant β-ionone apocarotenoid for enhanced aroma properties in wine FEMS Yeast Res. (IF 3.2) Pub Date : 2023-01-25 J J B Timmins, N van Wyk, H Kroukamp, R S K Walker, S Fritsch, D Rauhut, C von Wallbrunn, I S Pretorius, I T Paulsen
Wine is composed of multitudinous flavour components and volatile organic compounds that provide this beverage with its attractive properties of taste and aroma. The perceived quality of a wine can be attributed to the absolute and relative concentrations of favourable aroma compounds; hence, increasing the detectable levels of an attractive aroma, such as β-ionone with its violet and berry notes,
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Elevated energy costs of biomass production in mitochondrial respiration-deficient Saccharomyces cerevisiae FEMS Yeast Res. (IF 3.2) Pub Date : 2023-01-25 Pranas Grigaitis, Samira L van den Bogaard, Bas Teusink
Microbial growth requires energy for maintaining the existing cells and producing components for the new ones. Microbes therefore invest a considerable amount of their resources into proteins needed for energy harvesting. Growth in different environments is associated with different energy demands for growth of yeast Saccharomyces cerevisiae, although the cross-condition differences remain poorly characterized
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Identification of the main proteins secreted by Kluyveromyces marxianus and their possible roles in antagonistic activity against fungi FEMS Yeast Res. (IF 3.2) Pub Date : 2023-01-21 Ana Maria dos Santos, Fernanda Matias Albuini, Géssica Cabral Barros, Olinto Liparini Pereira, Wendel Batista da Silveira, Luciano Gomes Fietto
Lytic enzymes secreted by Kluyveromyces marxianus can lyse Saccharomyces cerevisiae cells. Their ability to hydrolyze yeast cell walls can be used in biotechnological applications, such as the production of glucans and protoplasts, as well as a biological control agent against plant pathogenic fungi. Herein, 27 proteins secreted by K. marxianus were identified by mass spectrometry analyses. Importantly
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The Influence of Torulaspora delbrueckii on Beer Fermentation FEMS Yeast Res. (IF 3.2) Pub Date : 2023-01-21 Firuze Kayadelen, Bilal Agirman, Neil P Jolly, Huseyin Erten
In this study, the effect of five different Torulaspora delbrueckii strains in combination with an ale type Saccharomyces cerevisiae on physical, chemical, microbiological, aroma composition and sensory profiles of beer were examined. The ethyl alcohol content of produced beers ranged from 5.46% (v/v) to 5.93% (v/v), while the highest alcohol amount was obtained using a pure culture of Saccharomyces
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pCEC-red: a new vector for easier and faster CRISPR-Cas9 genome editing in Saccharomyces cerevisiae FEMS Yeast Res. (IF 3.2) Pub Date : 2023-01-14 Letizia Maestroni, Pietro Butti, Vittorio Giorgio Senatore, Paola Branduardi
CRISPR-Cas9 technology is widely used for precise and specific editing of Saccharomyces cerevisiae genome to obtain marker-free engineered hosts. Targeted Double Strand Breaks (DSB) is controlled by a guide RNA (gRNA), a chimeric RNA containing a structural segment for Cas9 binding and a 20-mer guide sequence that hybridises to the genomic DNA target. Introducing the 20-mer guide sequence into gRNA
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The prevalence of killer yeasts and double-stranded RNAs in the budding yeast Saccharomyces cerevisiae. FEMS Yeast Res. (IF 3.2) Pub Date : 2023-01-04 Angela M Crabtree,Nathan T Taggart,Mark D Lee,Josie M Boyer,Paul A Rowley
Killer toxins are antifungal proteins produced by many species of "killer" yeasts, including the brewer's and baker's yeast Saccharomyces cerevisiae. Screening 1270 strains of S. cerevisiae for killer toxin production found that 50% are killer yeasts, with a higher prevalence of yeasts isolated from human clinical samples and winemaking processes. Since many killer toxins are encoded by satellite double-stranded
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Retraction of: Transcription factor Liv4 is required for growth and pathogenesis of Cryptococcus neoformans. FEMS Yeast Res. (IF 3.2) Pub Date : 2023-01-04
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Expanding the genome editing toolbox of Saccharomyces cerevisiae with the endonuclease ErCas12a. FEMS Yeast Res. (IF 3.2) Pub Date : 2023-01-04 Nicole X Bennis,Jonah P Anderson,Siebe M C Kok,Jean-Marc G Daran
ErCas12a is a class 2 type V CRISPR-Cas nuclease isolated from Eubacterium rectale with attractive fundamental characteristics, such as RNA self-processing capability, and lacks reach-through royalties typical for Cas nucleases. This study aims to develop a ErCas12a-mediated genome editing tool applicable in the model yeast Saccharomyces cerevisiae. The optimal design parameters for ErCas12a editing
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Zuo1, a ribosome-associated J protein, is involved in glucose repression in Saccharomyces cerevisiae. FEMS Yeast Res. (IF 3.2) Pub Date : 2023-01-04 Yoichi Yamada,Atsuki Shiroma,Suguru Hirai,Jun Iwasaki
In Saccharomyces cerevisiae, the J-protein Zuo1 and the nonconventional Hsp70 homologue Ssz1 stimulate the ATPase activity of the chaperone proteins Ssb1 and Ssb2 (Ssb1/2), which are associated with the ribosomes. The dephosphorylation of sucrose nonfermenting 1 (Snf1) on Thr210 is required for glucose repression. The Ssb1/2 and 14-3-3 proteins Bmh1 and Bmh2 appear to be responsible for the dephosphorylation
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A new hypothesis for the origin of the lager yeast Saccharomyces pastorianus. FEMS Yeast Res. (IF 3.2) Pub Date : 2023-01-04 Mathias Hutzler,John P Morrissey,Andreas Laus,Franz Meussdoerffer,Martin Zarnkow
Saccharomyces pastorianus, which is responsible for the production of bottom-fermented lager beer, is a hybrid species that arose from the mating of the top-fermenting ale yeast Saccharomyces cerevisiae and the cold-tolerant Saccharomyces eubayanus around the start of the 17th century. Based on detailed analysis of Central European brewing records, we propose that the critical event for the hybridization
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How to think about and do successful research What you probable did not learn when you first entered the laboratory. FEMS Yeast Res. (IF 3.2) Pub Date : 2023-01-04 Terrance G Cooper
There is a logic to doing successful research, but graduate students and indeed postdoctoral fellows and young independent investigators often learn it apprentice style, by experience. The purpose of this essay is to provide the product of that experience and advice that I have found useful to young researchers as they begin their training and careers.
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Grape-associated fungal community patterns persist from berry to wine on a fine geographical scale FEMS Yeast Res. (IF 3.2) Pub Date : 2023-01-03 Jonathan T Martiniuk, Jonah Hamilton, Thomas Dodsworth, Vivien Measday
Wine grape fungal community composition is influenced by abiotic factors including geography and vintage. Compositional differences may correlate with different wine metabolite composition and sensory profiles, suggesting a microbial role in the shaping of a wine's terroir, or regional character. While grape and wine-associated fungal community composition has been studied extensively at a regional